First mtDNA Sequences and Body Measurements for Rattus norvegicus from the Mediterranean Island of Cyprus

Invasive species are the primary driver of island taxa extinctions and, among them, those belonging to the genus Rattus are considered as the most damaging. The presence of black rat (Rattus rattus) on Cyprus has long been established, while that of brown rat (Rattus norvegicus) is dubious. This study is the first to provide molecular and morphological data to document the occurrence of R. norvegicus in the island of Cyprus. A total of 223 black rats and 14 brown rats were collected. Each sample was first taxonomically attributed on the basis of body measurements and cranial observations. Four of the specimens identified as R. norvegicus and one identified as R. rattus were subjected to molecular characterization in order to corroborate species identification. The analyses of the mitochondrial control region were consistent with morphological data, supporting the taxonomic identification of the samples. At least two maternal molecular lineages for R. norvegicus were found in Cyprus. The small number of brown rats collected in the island, as well as the large number of samples of black rats retrieved in the past years might be an indication that the distribution of R. norvegicus is still limited into three out of the six districts of Cyprus.


Introduction
Rattus rattus Linnaeus, 1758 (black rat) and Rattus norvegicus Berkenhaut, 1769 (brown rat) are considered as the most damaging and successful invasive species on the planet. Due to their commensality with humans they have spread in almost all regions of the world, with R. rattus occurring on all continents and R. norvegicus excluded only from Antarctica [1][2][3]. Invasive species, and more precisely species of the genus Rattus, are known to negatively affect island biota [4][5][6] and are the primary drivers of species extinctions on islands [7][8][9]. Due to the overall dramatic impact of rat invasions on autochthonous biota and on public health, the taxonomic identification of Rattus species spreading in specific locations, with particular reference to islands, are of pivotal importance for the conservation of local endemism [10,11]. The genus Rattus includes 66 species [3]; therefore, its taxonomy is complex being further complicated by a plethora of synonyms for different species [12]. Identification of individuals, even by experts, is often difficult [13], and for this reason the analysis of morphological traits combined with the genetic characterization of molecular markers is the most efficient system for a well addressed species taxonomic identification [3]. In such a context, the primary aim of this study is the assessment of the presence of this species on the island, thus providing the first morphological characterization for individuals of R. norvegicus in Cyprus. In order to corroborate the morphological identification, a molecular taxonomic identification was also performed for several specimens, thus providing the first molecular data for this species in the island.

Sample Collection
The number of rats collected and identified for each species is presented in Table 1, and the sampling sites are indicated in Figure 1. Rats were generally collected using snap traps and Herman live traps from January 2019 up to June 2020. Samples were also collected from animals found dead on the road. In addition, 18 skulls of R. rattus were collected from five barn owl nests, located at Xylophagou Larnaca, Machaeras Monastery, Kissonerga Paphos, Polis Paphos, and Vavatsinia Larnaca ( Table 1). Table 1. Sampling plan for the brown rats and black rats collected in the present study. + visual observation, ++ visual observation, dead in the nest of a long-legged Buzzard (Buteo rufinus), * road killed, ** captured in snap traps or live traps, +++ skulls collected in barn owl pellets. Black rats were identified from brown rats based on the characteristics descripted by Kryštufek and Vohralík [24], Nagorsen [32], Qumsiyeh [33], and Yigit et al. [34]. Since in most of the road-killed individuals part of the animal was smashed, one of the characteristics which remained unchanged and allowed instant identification of specimens was the colors of the tail. According to Kryštufek and Vohralík [24], in R. rattus the tail is uniformly black, whereas in R. norvegicus the tail is indistinctly bicolored, greyish brown above, pale below. For the identification of the species from the skulls found in owl pellets, only complete skulls were used, with intact parietal and interparietal ridges and occipital condyles. A mitochondrial DNA sequencing analysis was carried out in four rats collected between September and December 2019 from three different locations of Cyprus which showed a R. norvegicus phenotype (see Table 1 and Figures 1-3 for more details) with the aim to support the species identification performed on morphological bases ( Table 2). The same molecular analysis was also carried out for one of the samples of R. rattus in order to perform a comparative molecular phylogenetic analysis. Pictures of a brown rat and a black rat, respectively, taken in Cyprus during the present study, are shown in Figures 2 and 3.

. Examination of Live Specimens and Carcasses
In April 2019, an adult male was collected dead on the road at the center of the village Ne orio near Kythrea. A hair sample was collected for molecular analysis (RnC1 in Table 2). The tw ecimens from Geri, one specimen from Kelia village and one from Geri livestock farms, wer inned in the standard museum manner. From a fifth specimen from Aradippou, the body wa maged and only the head was skinned and preserved. Examination of the stomachs of tw ecimens from Aradippou and Kelia, indicated that the animals fed on livestock food. Thes dividuals were collected near cow farms. The specimen from Neo Chorio was in an advanced stag decomposition and after taking body measurements and cleaning the skull, it was disposed. Bod easurements (head and body and tail length) were taken using a Tiger 771,588 Vernier Caliper 0 0 mm. For the measurements of the ears and the hind foot, a powerfix digital caliper, versio /2010 was used. For the weight a portable electronic balance was used, measuring to the neare am. For the observation of the color of the tail a Jeweller's loupe (handheld lens) 30 × 21 mm wa ed.
One of the authors of the present study (G.K.) for the last few years has been visiting the M ilia Sewage Treatment ponds for wildlife photography. He observed that in the ponds there wer ts having the characteristics of R. norvegicus, as described by Nagorsen [32]. These rats, whe mpared with R. rattus individuals, showed smaller ears and eyes, blunt nose, thick heavy bod d tail shorter than head and body (see Figure 2). G. Konstantinou and E. Hadjisterkotis (authors o is study) visited the area in order to photograph these rats and to visually compare their extern aracteristics with the rats collected at Neo Chorio and Geri (RnC1 and RnC2 in Table

Examination of Live Specimens and Carcasses
In April 2019, an adult male was collected dead on the road at the center of the village Neo Chorio near Kythrea. A hair sample was collected for molecular analysis (RnC1 in Table 2). The two specimens from Geri, one specimen from Kelia village and one from Geri livestock farms, were skinned in the standard museum manner. From a fifth specimen from Aradippou, the body was damaged and only the head was skinned and preserved. Examination of the stomachs of two specimens from Aradippou and Kelia, indicated that the animals fed on livestock food. These individuals were collected near cow farms. The specimen from Neo Chorio was in an advanced stage of decomposition and after taking body measurements and cleaning the skull, it was disposed. Body measurements (head and body and tail length) were taken using a Tiger 771,588 Vernier Caliper 0-300 mm. For the measurements of the ears and the hind foot, a powerfix digital caliper, version 11/2010 was used. For the weight a portable electronic balance was used, measuring to the nearest gram. For the observation of the color of the tail a Jeweller's loupe (handheld lens) 30 × 21 mm was used.
One of the authors of the present study (G.K.) for the last few years has been visiting the Mia Milia Sewage Treatment ponds for wildlife photography. He observed that in the ponds there were rats having the characteristics of R. norvegicus, as described by Nagorsen [32]. These rats, when compared with R. rattus individuals, showed smaller ears and eyes, blunt nose, thick heavy body, and tail shorter than head and body (see Figure 2). G. Konstantinou and E. Hadjisterkotis (authors of this study) visited the area in order to photograph these rats and to visually compare their external characteristics with the rats collected at Neo Chorio and Geri (RnC1 and RnC2 in Table 2, respectively).
Sample identification was based on the following diagnostic external characteristics that allow to distinguish R. norvegicus from R. rattus [24,32-34]: 1.
The tail is always shorter than head and body length, indistinctly bicolored, greyish brown above, pale below, ears do not reach the eyes when laid forwards: R. norvegicus, Norway rat, brown rat.

2.
The tail length is always greater than head and body length, uniformly black. When the ears are drawn forward, they reach the eyes and usually cover them: R. rattus, black rat.
Species were also recognized based on the diagnostic skull characteristics of R. norvegicus and R. rattus [24,[32][33][34], with particular reference to the shape of the supraorbital ridges. In R. rattus supraorbital ridges diverged posteriorly along parietals and close to occipital region they are slightly bent or curved, while in R. norvegicus they ran mainly parallel along parietals ( Figure 4). According to Pimsai et al. [35] the skull ridges are well-defined in older individuals and characteristic in shape; they are relatively straight and situated close to one another, which gives the impression of a relatively narrow braincase. As it is described by Nagorsen [32], in the Norway rat the braincase is rectangular, temporal ridges straight and nearly parallel, whereas in black rat the braincase is rounded, and the temporal ridges curved.
Life 2020, 10, x 6 of 16 to Pimsai et al. [35] the skull ridges are well-defined in older individuals and characteristic in shape; they are relatively straight and situated close to one another, which gives the impression of a relatively narrow braincase. As it is described by Nagorsen [32], in the Norway rat the braincase is rectangular, temporal ridges straight and nearly parallel, whereas in black rat the braincase is rounded, and the temporal ridges curved. The palate of R. norvegicus is broader behind, bullae are relatively smaller, and the incisive foramen slightly shorter than in R. rattus-(see [24]: p. 135, Figure 107 for R. norvegicus skull and p. 125, Figure 100 for R. rattus). In addition, the skull of R. rattus is also more elongated than that of R. norvegicus [33] and the occipital condyles are the most posteriorly projecting point of the skull [34].

DNA Extraction, Amplification, and Sequencing
Genomic DNA was extracted from the hairs of five samples (see Table 2 for details) by means of the InstaGene™ Matrix (Bio-Rad) according to the manufacturer's protocol. Sample quality and DNA concentration were determined via spectrophotometry using a ND-8000 (NanoDrop Technologies, Thermo Fisher Scientific Inc., Wilmington, DE, USA). The DNA mean concentration obtained was 50 ng/μL.
A pair of primers was designed by the authors by means of the "Web Primer: DNA and Purpose Entry" bioinformatic tool available at http://www.candidagenome.org/cgi-bin/compute/web-primer [36] and used to amplify a region of the R. norvegicus mitogenome encompassing the first 434 base pairs of the D-loop region (HVS-I domain): Rat_DFw (5′-ccatcaacacccaaagctgat-3′), Rat_DRv (5′cgagatgtcttatttaagggg-3′).
A standard 50 μL PCR mixture was used, including 250 ng DNA template, 2.5 mM MgCl2, 0.20 mM each dNTP, 0.20 μM each primer, 1× PCR buffer, and 2 units Taq DNA Polymerase (Sigma- The palate of R. norvegicus is broader behind, bullae are relatively smaller, and the incisive foramen slightly shorter than in R. rattus-(see [24]: p. 135, Figure 107 for R. norvegicus skull and p. 125, Figure 100 for R. rattus). In addition, the skull of R. rattus is also more elongated than that of R. norvegicus [33] and the occipital condyles are the most posteriorly projecting point of the skull [34].

DNA Extraction, Amplification, and Sequencing
Genomic DNA was extracted from the hairs of five samples (see Table 2 for details) by means of the InstaGene™ Matrix (Bio-Rad) according to the manufacturer's protocol. Sample quality and DNA concentration were determined via spectrophotometry using a ND-8000 (NanoDrop Technologies, Thermo Fisher Scientific Inc., Wilmington, DE, USA). The DNA mean concentration obtained was 50 ng/µL. A pair of primers was designed by the authors by means of the "Web Primer: DNA and Purpose Entry" bioinformatic tool available at http://www.candidagenome.org/cgi-bin/compute/webprimer [36] and used to amplify a region of the R. norvegicus mitogenome encompassing the first 434 base pairs of the D-loop region (HVS-I domain): Rat_DFw (5 -ccatcaacacccaaagctgat-3 ), Rat_DRv (5 -cgagatgtcttatttaagggg-3 ).
A standard 50 µL PCR mixture was used, including 250 ng DNA template, 2.5 mM MgCl 2 , 0.20 mM each dNTP, 0.20 µM each primer, 1× PCR buffer, and 2 units Taq DNA Polymerase (Sigma-Aldrich), furthermore, 25 µg of bovine serum albumin (BSA) (5 ng/mL) was also added to the reaction mixture. PCR amplifications were carried out on a GeneAmp PCR System 9700 (Applied Biosystems) under the following conditions: initial denaturation of 95 • C for 5 min, followed by 35 cycles of 95 • C for 50 s, 54 • C (annealing temperature) for 50 s, and 72 • C for 1 min; a final extension of 72 • C for 4 min was also applied. At the end, a post-treatment of 5 min at 72 • C and a final cooling at 4 • C were carried out. Both positive (i.e., a known sample of Rattus sp. DNA used to verify that the primers have attached to the DNA strand) and negative controls were used to test the effectiveness of the PCR protocols, and the absence of possible contamination. Electrophoresis was carried out on 2% agarose gels, prepared using 1× SBA buffer (Sodium Boric Acid, pH 8.2) and stained with Gel Red Nucleic Acid Stain (Biotium). PCR products were purified by ExoSAP-IT (USB Corporation) and sequenced for both forward and reverse strands (by means of the same primers used for PCR), using an external sequencing core service (Macrogen The Netherlands). PCRs and sequencing were repeated twice for each sample in order to verify the reliability of results. The PCR products did not show occurrence of aspecificity, excluding the possibility of multiple nuclear mtDNA-like sequences, furthermore, dual peaks of similar height, which could be interpreted as evidence of possible heteroplasmy, were not observed in any of the electropherograms.

Dataset Creation, Sequence Comparisons, and Species Identification
The recovered sequences were identified through BLAST analysis in the GenBank nucleotide database (NCBI) (http://www.ncbi.nlm.nih.gov/) in order to estimate the statistical significance of matches and corroborate the taxonomic attribution performed for the specimens on morphological bases.
The amount of genetic variation among R. norvegicus sequences, including the number of polymorphic sites (S) and haplotypes (H), the haplotype diversity (Hd), and nucleotide diversity (π), were estimated using DnaSP 6.10.03 [37].
Sequences were aligned using the software Clustal X2 [38] and a dataset including the sequences obtained in the present study for R. norvegicus (4) and R. rattus (1) along with the haplotypes deposited in GenBank for these species which showed the higher levels of similarity at the BLAST analysis (56 for R. norvegicus and 39 for R. rattus, see Figure 5 for GenBank accession numbers) was constructed.
A phylogenetic tree analysis was performed using the Bayesian Inference (BI) method and a Mus musculus sequence (GB # KY018919) was used as outgroup [39]. MEGA 7.0.14 [38] was used to infer the best nucleotide substitution model. The Bayesian tree was inferred using Mr Bayes 3.2.5 [40] via Markov chain Monte Carlo (MCMC). Samples were drawn every 1000 steps over 20,000,000 MCMC steps. The first 10% were discarded as burn-in. Acceptable sampling and convergence to the stationary distribution were checked by inspection of traces using Tracer 1.7 [41] and tree topology was edited in  Table S1.

Morphological Analysis
The preliminary body measurements, the shape of the skulls, the robustness of the body, and the length and color of the tail of each individual allowed to discriminate between R. norvegicus and R. rattus, the latter being slimmer with relatively larger ears and a longer black tail (Table 3).   Table S1.

Morphological Analysis
The preliminary body measurements, the shape of the skulls, the robustness of the body, and the length and color of the tail of each individual allowed to discriminate between R. norvegicus and R. rattus, the latter being slimmer with relatively larger ears and a longer black tail (Table 3). Table 3. This table reports the morphological diagnostic characters that were considered for the analysis. Body measurements in millimetres of nine R. norvegicus from Turkey and 63 R. rattus from Turkey and Cyprus, respectively ( [24] and references therein), were compared with the specimens from Cyprus here analyzed. The measurements of weight in R. norvegicus reported by Kryštufek and Vohralik [24] refer to one specimen only, therefore, no range of values (Min-Max) was provided.

External Characteristics
The number of rats collected and identified for each species is presented in Table 1. In addition, 18 skulls of R. rattus were collected from five barn owl nests, located at Xylophagou village of Larnaca (one skull), Machaeras Monastery, Troodos mountains (four skulls), Kissonerga Paphos (six skulls), Polis tis Chrysochous Paphos (two skulls), and Vavatsinia Larnaca (five skulls).
The brown rats were robust and heavily built, compared to slender black rats. The tail length of the brown rats was less than head and body (Table 3), whereas in black rats was longer than head and body.
The color of the brown rats on the back is a mixture of dull ochraceous brown to dark brown and slate grey; it is interspersed by black tips, and occasional white and black hairs. Black hairs are coarser than the soft buff brown hairs. Head, shoulder, and spine are darker; flanks are more buff grey; belly is greyish white of varying intensity. The outer surface of the ears is covered with short, sparse, blackish brown hairs. The inner surface of the ear is naked, pinkish white, with the edges and inner surfaces covered with short, sparse, brownish or whitish hairs. The base of the ear is covered with longer brownish hairs. The upper sides of both the fore and hind feet are covered with tiny whitish hairs, the nails are not pigmented, and the soles of the fore and hind feet are completely naked. When the ear is bend forward towards the eye, is not reaching the eye, or sometimes just reaching the eye. The hairs on the belly, the chest, and the chin are buff white to grey, the bases are greyish to dirty white.
The color of the black rats resembled what Kryštufek and Vohralík [24] considers frugivorous or alexandrines type. In the frugivorous type has pale (white to light grey, yellowish white or pale buff) and sharply defined belly, and dark back which is blackish brown, dark grey brown, reddish brown, or light brown. In the alexandrinus type the back is dark to light brown or grey and belly is darker than in the previous type, albeit clearly paler than back; flanks are frequently grey and demarcation is faint. All possible intermediate stages connect these color types. The tail of the R. norvegicus is indistinctively bicolored, due to the tiny dark bristles above and whitish bristles below, which gives the bicolored appearance. The tail bristles of 205 specimens of R. rattus examined from the districts Famagusta, Larnaca, Nicosia, Limassol, and Paphos, were all dark, giving the one color appearance.

Cranial Characteristics
The skull of brown rats is considerably robust, the braincase is narrow and elongated. The rostrum is moderately long, and the nasals are rounded off posteriorly. The parietals and interparietal are bordered by well-defined ridges which are straight and almost parallel, which gives the impression of a relatively narrow braincase. The squamosal and maxillary process of the zygomatic arc are laterally widened. The lacrimals project slightly from behind the infraorbital foramen, which is laterally and vertically broadened. The supraoccipital is nearly vertical, and the exoccipital condyles form the most posteriorly projecting point of the skull. In the black rats the skull is strong and heavily built, and supraorbital ridges diverge posteriorly along parietals; close to occipital region they are slightly bent, forming a distinctive rounded braincase. A morphological comparison of the cranial characteristics between black and brown rats is provided in Figure 4.

DNA Analysis
A 483-base pair-long fragment of the hypervariable domain I of the mitochondrial D-loop region from four R. norvegicus and one R. rattus specimens were sequenced and deposited in GenBank (GB# MN115391-94). A BLAST search for homologous sequences corroborated the taxonomic attribution performed for these individuals on morphological bases. A dataset was constructed including all the haplotypes with higher level of similarity at the BLAST analysis so far available for R. norvegicus and R. rattus (see Figure 5 for GenBank accession numbers). The Bayesian tree analysis ( Figure 5) provided a topology where the two Rattus species formed two well-supported monospecific and monophyletic clades, representing R. norvegicus (branches colored in blue in the figure) and R. rattus (branches colored in green), respectively ( Figure 5).
Three different maternal lineages (A, B, and C, see Figure 5) were detected for the R. norvegicus early radiation. Three sequences RnC1, RnC3, and RnC4 grouped within the clade B. RnC1 and RnC4 showed the same haplotype and clustered together with a sequence obtained from a wild brown rat captured in 2007 in French Polynesia (EF186347), while RnC3 showed a haplotype never described before. On the other hand, the Cypriot brown rat RnC2 was phylogenetically related to sequences belonging to the clade A. The Cypriot black rat RrC1 sequence was grouped within the R. rattus clade.
Overall, a total of 13 haplotypes, defined by 20 polymorphic sites, were detected for R. norvegicus ( Table 4). Eight of them were previously reported by Liu et al. 2017 [42] in brown rats from China (D1, D2, D4, D5, D12, D23, D24, D25 in Table 4). The three haplotypes found for the individuals of R. norvegicus from Cyprus resulting in nine polymorphic sites and high levels of genetic divergence (Hd = 0.833 π = 0.011). Estimates of genetic diversity among haplotypes are reported in Table 4.

Discussion
Identification of individuals belonging to species of Rattus, is often difficult [13], and for this reason the analysis of morphological traits combined with the genetic characterization of molecular markers represents the most efficient system for a well addressed species taxonomic identification [3]. Although the morphological criteria used for the first identification of an individual may vary over time, distinguishing among R. rattus and R. norvegicus is not so difficult, since according to Kryštufek and Vohralík [24], Nagorsen [32], Qumsiyeh [33], and Yigit et al. [34], as well as our observations in this study, in R. norvegicus the tail is always shorter than head and body length, ears do not reach the eyes when laid forwards, and the tail is bicolored. Although Kryštufek and Vohralík [24], notes that the tail of R. norvegicus is indistinctly bicolored, grayish brown above, pale below, based on our observations the tail in this species is distinctively bicolored, greyish brown above, whitish below. The distinctive Life 2020, 10, 136 11 of 16 discrepancy with previous descriptions is due to the different color of the tail bristles above and below the tail. On the contrary, in all R. rattus individuals the tail bristles above and below were black. This is the first confirmation based on cranial and body measurements as well as on molecular analysis of the presence of R. norvegicus on the island of Cyprus. We also present the first study on the variation of brown rats in Cyprus, providing the first preliminary molecular and morphological data on this alien invasive species, which can become devastating for agriculture and local wildlife. Cyprus is the only center of endemism for birds in Europe [43], a center of endemism for terrestrial mammals [27,29,30], reptiles [44], insects [45,46], terrestrial gastropods [46], and plants [47][48][49][50][51], and represents a biodiversity 'hotspot' [20,21,[52][53][54]. Biological diversity faces many threats throughout the world, and one of the major threats to native biological diversity is now acknowledged by scientists and governments to be biological invasions caused by alien invasive species such as rats. The impact of invasive species is immense, insidious, and usually irreversible. They may be as damaging to native species and ecosystems on a global scale as the loss and degradation of habitats [53].
The limited number of brown rats so far collected in Cyprus is an indication that the distribution of R. norvegicus is limited in the district of Nicosia and Larnaca, around the cities of Aradippou, Geri, Nicosia, and Larnaca, as well as the village of Neo Chorio Kythrea, and the Sewage Treatment Ponds of Mia Milia (Figure 3). In these restricted areas brown rats were found in the sewage treatment plant canals suggesting the possibility that they might spread into new locations following the canals. A similar situation was observed in Israel, where brown rats are more commensal than their relative the black rat but are less widespread. They penetrated human habitations of port cities and managed to reach some cities of the interior. R. norvegicus shows to prefer more mesic areas than R. rattus. Barrows can be found along stream banks (including sewage streams) in many areas [33].
The ports that are near to the localities where brown rats were collected are the ports of Famagusta and Larnaca, which are about 65 km from Nicosia. Therefore, finding brown rats in the District of Nicosia is an indication that they are spreading toward urban areas where they live around buildings as it was observed in the city of Geri, as well as in the center of Neo Chorio near Kythrea.
The island of Cyprus represents a further case study, where an alien highly invasive species (whose presence on the island was considered as dubious by several researchers), which can be a significant driver of population declines and species extinctions was documented for the first time in the present study. Black rats, according to Hadjisterkotis [29,30], occupy just about every rural habitat in Cyprus. The same was reported by Krystufek and Vohralik [24] who noted that "throughout Turkey and Cyprus, the black rat occupies dwellings (houses, granaries, stables and farm buildings, mills and so forth). Along the Aegean and the Mediterranean coasts of Anatolia and in Cyprus it is abundant and widespread also outside buildings, either in urban and suburban areas (parks, gardens, orchards), in cultivated areas (hedgerows, along fields), and in various types of shrubland, but is most common in humid places and in high maquis". Hadjisterkotis [55] also observed that black rat is common in forested areas with pine Pinus brutia and black pine Pinus nigra, as well as golden oak Quercus alnifolia, and in torrential river beds on the mountains, where it finds cover in bramble (Rubus sanctus), Smilax asperea, and myrtle Myrtus communis. This species is highly dispersed even on pine trees, where the species uses old nests of woodpigeons as platforms to brake pinecones to extract the seeds [55]. They are excellent climbers and occasionally they prefer to build large squirrel-like nests in dense bramble or even pine trees, and occasionally carob trees (Ceratonia ciliqua). Nests, generally at height of 2-3.5 m, are loosely made of leaves and twigs of the available trees or vegetation. The entrance is at one side of the nest, which is flatly domed like a squirrel's drey, and, from casual inspection, appears to be nothing more than a bunch of dead leaves and twigs, which the thickness of the vegetation keeps in place. In Cyprus during the summer, black rats are a major destruction for carob trees since they eat the fruit and peel the branches, consequently killing the trees. For the first time we also observed them in 2019 and 2020 building roughly round bulky nests on olive trees (Olea europaea), by cutting and pilling large numbers of olive tree twigs. In addition, we observed them eating the cambium layer of olive trees, in a similar way that they do with carob trees. Particularly selected for attack are fresh shoots, stripping branches up to 45 mm in diameter. Caches of olives were found under thick vegetation of rock roses (Cistus sp.), under stones and wooden boards, in hollow olive trees, even in tunnels under dense grass. Cashes of almonds were found in the hollow branches of almond trees, in abandoned farm buildings, and underneath large rocks (E.H. personal observation).
Black rats are well adapted to small islands, whereas brown rats are mostly observed on the largest islands where humans also occur [56][57][58]. Considering that Cyprus is the third largest island in the Mediterranean, brown rats are expected to adapt and to compete well with black rats thus exerting a stronger impact on native species.
Besides the above reported destruction of trees and crops, rats are particularly harmful in and around farm facilities where they seek food and refuge indoors, gnaw on structural, mechanical, and electrical components, weaken concrete slabs and walkways with their burrowing activities [58]. If not promptly eradicated, they can reach huge numbers, as in the case of a chicken farm in Chloraka, where we trapped 160 black rats.
Rats are reservoirs and vectors of pathogens [55,59,60] that can infect livestock, wildlife species, and humans [58]. A costly disease that was found to infect farm animals and rats, is paratuberculosis that causes significant economic losses for the farm industry with annual estimates of millions of dollars around the world. It was estimated that paratuberculosis costs the U.S. dairy industry alone 200-250 million dollars annually [60]. The etiologic agent Mycobacterium avium ssp. paratuberculosis (MAP) has been isolated from Cypriot cattle, sheep, and goat populations and dairy food [61][62][63]. Several studies revealed MAP isolations from brown rats [64], foxes, and feral cats [65]. The latter two species are predators of rats on Cyprus, which can become infected by feeding on them. These species may live for several years with home ranges that cover areas large enough to include more than one farm, spreading the disease from farm to farm, and re-infecting farms which eliminated all infected livestock. Therefore, the role of wildlife species (also including both species of rats) in the spreading of MAP to cattle, sheep, and goats must be examined, in order to take the proper management measures for the protection of livestock and humans. Besides the spreading of MAP to cattle, sheep, and goats, epidemiological and clinical data of 193 human cases of murine typhus in Cyprus were recorded and analyzed during a 9-year period (2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008). The data collected enhance the belief that murine typhus is a serious public health problem in Cyprus [66].
In the future, extensive studies focused on the demographic patterns of dispersal and habitat selection of R. norvegicus in Cyprus, will be needed to assess proper management plans for the protection of public health, agriculture, livestock, and biodiversity, and to depict, if any, the competitive interactions with R. rattus.
Furthermore, the expansion of R. norvegicus in the island should be also investigated with molecular markers on a large number of individuals to shed light on the phylogeographic structure of this species in Cyprus.