Experimental Study of Montmorillonite Structure and Transformation of Its Properties under Treatment with Inorganic Acid Solutions

This paper discusses the mechanism of montmorillonite structural alteration and modification of bentonites’ properties (based on samples from clay deposits Taganskoye, Kazakhstan and Mukhortala, Buriatia) under thermochemical treatment (treatment with inorganic acid solutions at different temperatures, concentrations and reaction times). Treatment conditions were chosen according to those accepted in chemical industry for obtaining acid modified clays as catalysts or sorbents. Also, more intense treatment was carried out to simulate possible influence at the liquid radioactive site repositories. A series of methods was used: XRD, FTIR, ICP-AES, TEM, nitrogen adsorption, and particle size analysis. It allowed revealing certain processes: transformation of montmorillonite structure which appears in the leaching of interlayer and octahedral cations and protonation of the interlayer and –OH groups at octahedral sheets. In turn, changes in the structure of the 2:1 layer of montmorillonite and its interlayer result in significant alterations in the properties: reduction of cation exchange capacity and an increase of specific surface area. Acid treatment also leads to a redistribution of particle sizes and changes the pore system. The results of the work showed that bentonite clays retain a significant portion of their adsorption properties even after a prolonged and intense thermochemical treatment (1 M HNO3, 60 ◦C, 108 h).


Introduction
Clay minerals are widely used in various industries including radioactive waste management as a component of barrier systems for waste disposal.These systems are used for radioactive waste repositories of different levels of activity and are intended to provide safe storage for several hundreds or thousands years due to their high adsorption capacity for radionuclides and low water permeability [1][2][3].
Bentonite buffer between containers and the tunnel walls achieves several strategic outcomes at once: it provides access restriction of groundwater to the radioactive waste (RW) creating the conditions under which mass transfer between the waste and underground waters is only possible by diffusion; it suppresses migration of the radionuclides in colloidal form into the groundwater; it ensures effective sorption of radionuclides after possible depressurization of radioactive waste containers; it seals open cracks and large pores in the rocks due to the high swelling capacity, etc. [2].
One of the main components of the engineered barriers is bentonite, which contains 70-95% montmorillonite, dioctahedral specie of the smectite mineral group.Montmorillonite is a 2:1 type hydrous aluminosilicate with the octahedral sheet "sandwiched" between two tetrahedral sheets.Cation substitution in tetrahedral and mostly in octahedral sites provides a negative layer charge of about 0.2-0.5 eV.Layer charge is compensated by the introduction of exchangeable interlayer cations (Na + , Ca 2+ , Mg 2+ , etc.) usually in hydrated form [4][5][6][7][8] which in turn provides adsorption sites on the inner and outer surface of the crystal.These particularities of montmorillonite structure determine specific properties of bentonite clays, especially high adsorption capacity towards heavy metals such as cesium, plutonium etc., which are commonly found in radioactive wastes.
Construction concept of waste disposal in the Russian Federation also includes the use of bentonite clay as a component of an engineered multi-barrier system [9,10].According to current concepts, a bentonite barrier must retain its properties for over thousands of years.Thus, when analyzing the prospects of using bentonite clays, one needs to consider not only their sorption properties in their natural state, but a possible loss of sorption capacity and other parameters needed to preserve the stability of a bentonite barrier in aggressive environments.The most aggressive environment for bentonite clays is acid solutions.A number of processes take place under acid treatment and lead to a significant transformation of the structure and properties of montmorillonite bentonite clays [11][12][13][14].A significant increase in the specific surface of the acid-modified clays promotes wide use of such material for removal of heavy metals, radionuclides and for oil refining [15][16][17][18][19].The transformation of the structure and properties of bentonite under the influence of acids, mostly under sulfuric and hydrochloric acids, have been studied by different authors [20][21][22][23][24][25][26].Using ultrasound and microwaves in addition to an acid treatment intensifies smectite mineral transformation [27].In industry, acid-modified bentonites are most commonly used as catalysts [28][29][30][31][32].
In Russia, studies have been conducted to identify the most thermochemically resistant bentonite in order to determine the most appropriate bentonite clay for engineered barriers for waste disposal.The increase of the temperature in the vicinity of radioactive waste occurs due to radioactive decay.Thermochemical effects were modeled based on inorganic acid solutions (nitric and hydrochloric) at elevated temperatures.Due to its toxicity, nitric acid is not used in the chemical industry for the production of modified materials.Currently in Russia, there are plants that still dump liquid radioactive waste into deep layers of geological structures and in artificial surface repositories [33][34][35][36][37]. Nitric acid is used to prepare liquid radioactive waste for disposal.These concepts are obsolete and are being withdrawn from use.Bentonite is also used to bury surface basins, such as Lake Karachai [38].
However, in the long run, bentonite clays may be affected by aggressive fluids that are most likely to decrease insulating properties of the barrier.The aim of this study was to evaluate the mechanism of montmorillonite transformation under the acid solution treatment as well as its influence on bentonite properties.

Materials and Methods
Fine clay fractions (<1 µm) separated from bentonites from Mukhortala (Buriatia, sample Mt-M) and Taganskoye (Kazakhstan, sample Mt-T1 and Mt-T2 (numbers "1" and "2" correspond to different treatments as will be shown below)) deposits were used for this study.Montmorillonite content in Mt-T and Mt-M sample were 70% and 97%, respectively.Impurities in Mt-M sample were presented by 20-30% opal C/A, which could not be removed with the sedimentation technique, and quartz (about 2-3%) in Mt-T sample.
All experiments on acid treatment were carried out in closed systems under static conditions.For this purpose, bentonite samples were placed in sealed vessels with acid solution in a weight ratio of 1:100.Vessels were in turn placed in an oven and aged for the desired time.The experiments with HCl solutions (0.125, 0.25, 0.5, 1, 3 M) were carried out at room temperature for 7 days (Mt-T1 and Mt-M samples) and with 1 M HNO 3 solutions (Mt-T2 sample) at 60 • C for 12, 36, 50, and 108 h.The bentonite samples from the Taganskoye deposit have quite a different composition and were named Mt-T1 for the experiment with HCl solution and Mt-T2 for the experiment with HNO 3 solution.Hydrochloric acid treatment was conducted under the conditions accepted in chemical industry for modified bentonite production.Experiments with nitric acid treatment were conducted at high temperatures in order to simulate the conditions that may occur during liquid radioactive waste disposal.
Initial and modified samples were analyzed with a series of methods.X-ray diffraction patterns were obtained with X-ray diffractometer Ultima-IV (Rigaku, Tokyo, Japan) acquired with the funding of Moscow State University Development Program (Cu-Kα radiation, semiconductor 1D detector D/Tex-Ultra, scan range 3-65 • (2θ), scan speed 5 • /min and step-0.02• (2θ)).Partially oriented specimens were prepared by pressing powder into a sample holder.
The chemical analyses of the solids were carried out by means of inductively coupled plasma atomic emission spectrometry (ICP-AES) using ICPE-9000 equipment (Shimadzu, Kyoto, Japan).
Excessive pressure and surface leveling leads to partial orientation of the montmorillonite particles in the specimen plane.We did not succeed in preparing samples with a good degree of particle orientation from drops of aqueous suspensions because some samples had been significantly modified after the treatment so they could not form thin films on a glass surface.The results were analyzed according to Drits, Kossovskaya [4], Moore and Reynolds [5].Mineral composition was estimated using the Rietveld method [39] with PROFEX GUI for BGMN [40].
Fourier transform infrared spectroscopy analysis was carried out using FTIR spectrometer Vertex 80v equipped by DTGS detector and KBr beam-splitter (Bruker, Ettlingen, Germany).The adsorption spectra recordings were performed in vacuum in the 4000-400 cm −1 wavelength range with 256 scans for each sample and the resolution of 4 cm −1 .Samples were prepared as pressed KBr-pellets: 0.5 mg of sample was dispersed in 200 mg of KBr; this mixture was placed in a 13 mm pellet die and pressed in vacuum for 1 h.Spectra manipulations were performed using the OPUS 7.1 software (Bruker, Ettlingen, Germany).Baseline correction was made automatically by Concave Rubberband method with 64 baseline points and 10 iterations.
Natural montmorillonites (Mt-M, Mt-T1, Mt-T2) as well as some samples after treatment were selected based on the results of X-ray diffraction studies (Mt-M-3.0M HCl; Mt-T1-0.25 M and 0.5 M HCl; 12 and 108 h) for further investigation by high-resolution transmission electron microscopy (HRTEM) using JEM-2100 with an X-Max attachment for X-ray energy dispersive analysis.
Evaluation of a specific surface area was carried out using the Analyzer Quadrasorb SI/Kr (Quantachrome Instruments, Boynton Beach, FL, USA).Adsorption was performed at the liquid nitrogen temperature (77.35 K).Nitrogen with a 99.999% purity served as an adsorbate.Helium grade 6.0 (99.9999%) was used for the volume calibration of the measuring cells.Calculation was carried out by the BET multiple-point isotherm in the range of P/P0 from 0.05 to 0.30.Samples were pre-dried in vacuum at 100 • C.
Particle size distribution was evaluated with the laser diffraction technique by Fritsch ANALYSETTE 22 NanoTec (Fritsch, Idar-Oberstein, Germany) equipped with 70 W and 36 kHz ultrasonic emitter in the size range 0.01-1000 µm Data processing was carried out by algorithm based on Fredholm integral equations.

Transformation of the Montmorillonite Structure under Acid Treatment
Considerable crystal-chemical transformations-in particular, changes of montmorillonite micromorphology and adsorption characteristics-were detected under treatment with hydrochloric and nitric acid solutions.A variety of processes that modified structure and properties of montmorillonite particles were observed during the treatment with inorganic acid solutions: dissolution of carbonates and feldspars, destruction of the most defective phyllosilicate phases (e.g., nanosized smectites), removal of cations from the interlayer spacing, substitution of interlayer cations with oxonium ion, leaching of the octahedral cations, and finally, the complete destruction of the structure.

Transformation of the Montmorillonite Structure under Acid Treatment
Considerable crystal-chemical transformations-in particular, changes of montmorillonite micromorphology and adsorption characteristics-were detected under treatment with hydrochloric and nitric acid solutions.A variety of processes that modified structure and properties of montmorillonite particles were observed during the treatment with inorganic acid solutions: dissolution of carbonates and feldspars, destruction of the most defective phyllosilicate phases (e.g., nanosized smectites), removal of cations from the interlayer spacing, substitution of interlayer cations with oxonium ion, leaching of the octahedral cations, and finally, the complete destruction of the structure.
Early, we demonstrated that the chemical composition and structural characteristics of Mt depended on the HNO 3 and HCl concentration [26,32].Changes of chemical composition and interlayer space were negligible after modification of montmorillonite under the concentrations up to 0.5 M.These changes were noticeable only after treatment with 3.0 M acid.Here we investigated effect of exposure time of 1M HNO 3 at 60 • C on chemical composition of Mt-T2.The main results are shown in the Table 2. Experimental data point that leaching of interlayer cation is observed after the treatment with 1 M HNO 3 for 12 h.Profile alignment of basal reflections (001) and displacement of maximum 13.7 Å to 14.0 Å was observed after treatment of Mt-T1 sample with hydrochloric acid solution.Irregular profile shape of d 001 reflection indicates the presence of two possible montmorillonite phases with different interlayer cations in the sample.This was also found by other researchers of the Taganskoye montmorillonite deposit [43].Since any other impurities except quartz were not found in this sample, the described change in the XRD patterns can be attributed to the partial destruction (dissolution) of the most defective and probably nanosized particles of Na-montmorillonite.
Treatment of Mt-T sample with 1M HNO 3 solution for 12 h leads to a displacement of the d 001 reflection from 13.9 to 13.2 Å with an increase of its intensity.This fact is explained by the dissolution of the defective part of the montmorillonite and enhancement of the interlayer ordering by partial substitution of Ca 2+ and Mg 2+ cations.Further treatment of the sample with nitric acid solution reduces the layer stacking ordering degree and leads to disintegration of montmorillonite 2:1 layer structure.In XRD patterns, the described process is manifested by a decrease in the peaks intensity and broadening of the basal reflections until the complete disappearance except (001) reflection.Displacement of the (001) reflection back to 13.9 Å after the 36 and 108 h of treatment can be explained by the Mg and Al octahedral cations leaching and their migration into the interlayer space of montmorillonite.
The changes in the sizes of the coherent scattering domains (CSD) along the c-axis were calculated in accordance with the Scherrer equation for the (001) reflection of natural and modified montmorillonites (Table 1).In general, the size of CSD along the c-axis corresponds with the crystallite thickness [46].The average crystallite thicknesses of the samples equals 10.7 nm which corresponds to 7 layers (7 N), 9.6 nm (7 N), 8.2 nm (6 N) for natural Mt-M, MtM-T1 and Mt-T2 samples respectively, and to 5.5-5.6 nm (4 N) for the acid treated montmorillonites.A decrease of CSD sizes is related to a consequent disintegration of montmorillonite particles and increase of the stacking faults quantity.
The increase of the adsorbed water bands on IR spectra in the range of 4000-2500 cm −1 probably indicates a decrease of crystallite size (Figure 2a) after the treatment of Mt-T2 sample with HNO 3 acid for 108 h.Previous studies of Mt-M and Mt-T1 samples treated with HCl acid [26] showed the reduction of band intensity at 841-845 cm −1 (Al-Mg-OH), 882 cm −1 (Al-Fe-OH) and 914-926 cm −1 (Al-Al-OH), which indicates the leaching of Al 3+ , Mg 2+ and Fe 3+ cations from the octahedral sites [44][45][46][47][48][49].Reduction of the band intensities at 925 and 876 cm −1 is shown at  Leaching of the octahedral cations leads to modifications of the interaction between the octahedral and tetrahedral sheets in the 2:1 layer and, as a result, to the partial destruction of octahedral sheets.Alterations in FTIR data revealed as decreasing intensities of the bands related to octahedral Al, Fe and Mg and exchangeable Ca, Na and Mg correlate with changes in chemical composition shown above.Therefore, the structural units comprised of the tetrahedral sheet fragments are released from the 2:1 structure, which corresponds to "Si-Ofree" vibrations-1095 cm −1 [11,15,21,50].Changes in the Si-O-Si band profile within the range of 1100-1050 cm −1 (Figure 2b) indicate transformation of interaction within the tetrahedral sheet.In particular, displacement of the maximum in the natural sample from 1050 to 1095 cm −1 after treating it with 1 M HNO3 for 108 h indicates the increase of amorphous silica content due to partial destruction of the tetrahedral sheet which is confirmed by chemical analysis data as an increase in Si content in powder samples.
Transmission electron microscopy (TEM) allows collection of data at the level of crystal lattice and helps to estimate structural transformation of montmorillonite, properties of surface and interactions between the layers during its treatment with inorganic acid solutions, and changes in montmorillonite particles' chemical composition [51].In natural samples from the Tagansoye deposit (Figure 3a) among the laminar montmorillonite particles with a size of 1-2 μm, there is a significant amount of small and thin nano-sized particles that cover the specimen and produce grey background in micrographs.Particle identification was carried out based on microdiffraction patterns and micro-area chemical analysis.The nanosized phase is characterized by a higher content of Na as an interlayer cation and Fe is located in the octahedral sheet due to the isomorphic substitution of Al.Under hydrochloric acid with lower concentrations, as well as under the treatment with nitric acid for shorter periods, almost full dissolution of nano-sized particles as the less acid resistant phase takes place and the grey background on micrographs is not observed.Besides, these laminar particles have a lot of folded edges which are not usually observed in acid-modified montmorillonites.Bentonites from Mukhortala deposit (Mt-M) contain a lot of opal distributed in pores between thin and relatively thick laminar montmorillonite particles and also on their relatively clean surfaces (Figure 3c).Leaching of the octahedral cations leads to modifications of the interaction between the octahedral and tetrahedral sheets in the 2:1 layer and, as a result, to the partial destruction of octahedral sheets.Alterations in FTIR data revealed as decreasing intensities of the bands related to octahedral Al, Fe and Mg and exchangeable Ca, Na and Mg correlate with changes in chemical composition shown above.Therefore, the structural units comprised of the tetrahedral sheet fragments are released from the 2:1 structure, which corresponds to "Si-O free " vibrations-1095 cm −1 [11,15,21,50].Changes in the Si-O-Si band profile within the range of 1100-1050 cm −1 (Figure 2b) indicate transformation of interaction within the tetrahedral sheet.In particular, displacement of the maximum in the natural sample from 1050 to 1095 cm −1 after treating it with 1 M HNO 3 for 108 h indicates the increase of amorphous silica content due to partial destruction of the tetrahedral sheet which is confirmed by chemical analysis data as an increase in Si content in powder samples.
Transmission electron microscopy (TEM) allows collection of data at the level of crystal lattice and helps to estimate structural transformation of montmorillonite, properties of surface and interactions between the layers during its treatment with inorganic acid solutions, and changes in montmorillonite particles' chemical composition [51].In natural samples from the Tagansoye deposit (Figure 3a) among the laminar montmorillonite particles with a size of 1-2 µm, there is a significant amount of small and thin nano-sized particles that cover the specimen and produce grey background in micrographs.Particle identification was carried out based on microdiffraction patterns and micro-area chemical analysis.The nanosized phase is characterized by a higher content of Na as an interlayer cation and Fe is located in the octahedral sheet due to the isomorphic substitution of Al.Under hydrochloric acid with lower concentrations, as well as under the treatment with nitric acid for shorter periods, almost full dissolution of nano-sized particles as the less acid resistant phase takes place and the grey background on micrographs is not observed.Besides, these laminar particles have a lot of folded edges which are not usually observed in acid-modified montmorillonites.Bentonites from Mukhortala deposit (Mt-M) contain a lot of opal distributed in pores between thin and relatively thick laminar montmorillonite particles and also on their relatively clean surfaces (Figure 3c).Studied samples of the natural montmorillonites are characterized by peculiar particle morphology that is typical for montmorillonites of different genesis and by a wide spread of welted edges, which can be useful for obtaining pictures of the lattices (Figure 4).Images obtained from edges of natural montmorillonite (Mt-T2 sample) show the lattice stripes that correspond to the basal planes (Figure 4a,b).The width of the areas with lattice stripes is about 23-32 nm.The value of the interlayer space for different particles varies within the range from 10.5 to 11.8-12.3Å.  Studied samples of the natural montmorillonites are characterized by peculiar particle morphology that is typical for montmorillonites of different genesis and by a wide spread of welted edges, which can be useful for obtaining pictures of the lattices (Figure 4).Images obtained from edges of natural montmorillonite (Mt-T2 sample) show the lattice stripes that correspond to the basal planes (Figure 4a,b).The width of the areas with lattice stripes is about 23-32 nm.The value of the interlayer space for different particles varies within the range from 10.5 to 11.8-12.3Å. Studied samples of the natural montmorillonites are characterized by peculiar particle morphology that is typical for montmorillonites of different genesis and by a wide spread of welted edges, which can be useful for obtaining pictures of the lattices (Figure 4).Images obtained from edges of natural montmorillonite (Mt-T2 sample) show the lattice stripes that correspond to the basal planes (Figure 4a,b).The width of the areas with lattice stripes is about 23-32 nm.The value of the interlayer space for different particles varies within the range from 10.5 to 11.8-12.3Å.The lattice stripes of different particles can be pulled simultaneously, maintaining a noticeable distance between the adjacent bands or showing some curving of the surface, which indicates only a small change of interlayer space along the layers.Moreover, there are areas of the image with two-dimensional lattice stripes (Figure 4b).Basal lattice stripes are crossed by lattice stripes with an interlayer space of 4.5 Å which corresponds to planes (020) and indicates local manifestations of coherence in stacking of adjacent layers.
Microaggregate surface of Mt-T2 sample became uneven as the result of the treatment with HNO 3 for 108 h.This effect can be seen only at some fragments of the images with folded edges with typical parallel basal lattice stripes from planes (00l).It correlates to the small packs of 2-3 layers (Figure 4b) less than 25 nm long.The interlayer spacing 12-14 Å indicates the presence of initial montmorillonite.The observed pattern of distribution of the basal lattice stripes reflects the nano-level changes in the surface morphology of montmorillonite.It implies an existence of thin packages of layers where the planes (00l) are arranged perpendicularly to the surface of the particles.This effect in particular leads to an increase of its surface and the occurrence of active sites for the selective sorption of heavy metals and radionuclides.

Transformation of Montmorillonite Adsorption Properties under Acid Treatment
Specific surface area (BET) and cation exchange capacity (CEC) can be qualified as characteristics of adsorption properties of bentonite clays.CEC values of montmorillonites depend most significantly on the amount of isomorphic substitutions in octahedral sites.In turn, specific surface area is primarily controlled by degree of fineness, amount of impurities, particle charge, their ability to coagulate, etc. Mt-M sample contains a significant amount of opal which provides a relatively higher specific surface area than that of Taganskoye deposit samples (Mt-T1 and Mt-T2).Commonly, specific surface area (BET) values are in direct relationship with CEC value [52].Increasing of BET value leads to an increase of CEC value and vice versa.In most cases, the above is true for natural soils with different mineral compositions [46].However, the ratio of these indexes for montmorillonites of different composition may be in a more complex relationship.This dependence can be clearly seen in the Table 1 as an increase of specific surface area and a decrease in cation exchange capacity due to the acid treatment.
Coherent scattering domain sizes are commonly used to analyze the size of crystallites [46,[53][54][55].There is a cumulative index of physical-chemical activity of the most clay minerals which have correlations with specific surface area and adsorption characteristics.Thus, reduction of crystallite size should lead to an increase of specific surface area, which is shown in the study (Table 1).
At the same time, an increase of the specific surface area appears mostly in the samples which have the greatest structural changes.Thus, in the montmorillonite sample from Taganskoye deposit, treated with HNO 3 for a long period of time, the increase of the specific surface area was 301 m 2 /g compared to 67 m 2 /g in the natural sample.Also, considerable changes were observed in the sample with montmorillonite from the Mukhortala deposit (from 77 to 101 m 2 /g, respectively).
The average pore size in all samples is approximately 5 nm and it remains constant during the experiments, while the total pore volume has been changed slightly for Mt-M and Mt-T1 samples and considerably so for Mt-T2 sample.This fact cannot be related only to the size decrease.In this way, the observed increase of specific surface area, as well as the total pore volume is related to the formation of porous structure due to the modification of montmorillonites under the acid treatment.
As a result of the treatment, a regular change is observed in the chemical composition of montmorillonite (Table 2).First of all, the reduction of Ca, Mg, Fe, and Al content and the increase of Si content is found in all samples after dissolving the nano-sized phases of montmorillonite from Taganskoye deposit.The most significant changes were found in the Mt-T2 sample treated with HNO 3 acid solution for 108 h.The increase of Si content in montmorillonite samples subjected to the intensive acid treatments connected with subsidence of amorphous silica formed during the destruction of tetrahedral sheets of 2:1 layers on the particle surface and its accumulation in the micropores.
Protonation of Al bond pairs in the octahedron transforms it from 4-coordination to 6-coordination [23] and leads to the modification of octahedral and tetrahedral sheets and to an appearance of micropores simultaneously decreasing the number of octahedral cations.Changes in the layer stacking and particles' micromorphology results in mesopore formation [14,45].Structural transformations are shown simplified in Figure 5. Thus, there is an increase of specific surface area and pore space both capable of large cation sorption, e.g., Cs.
Protonation of Al bond pairs in the octahedron transforms it from 4-coordination to 6-coordination [23] and leads to the modification of octahedral and tetrahedral sheets and to an appearance of micropores simultaneously decreasing the number of octahedral cations.Changes in the layer stacking and particles' micromorphology results in mesopore formation [14,45].Structural transformations are shown simplified in Figure 5. Thus, there is an increase of specific surface area and pore space both capable of large cation sorption, e.g., Cs.Critical values for describing the concepts of "micropores", "mesopores", and "macropores" vary in classifications developed for different science approaches.In engineering geology, for example, mesopore sizes are in the range of 10 to 1000 μm and micropores are from 0.1 to 10 μm [56].V. Osipov and V. Sokolov in their morphometric investigations of soil microstructures with different composition [52] detailed the previously proposed classification of pore sizes.They divided micropores into thin (0.1-10 μm), small (1-10 μm), and large (10-100 μm) ones, suggesting the lower limit for macropores as 100 μm.
There are other classifications to consider.For example, the International Union for Pure and Applied Chemistry (the IUPAC) recommended to distinguish pores size into macropores (50 nm), mesopores (2 to 50 nm), and micropores (up to 2 nm) [57][58][59].The micropores are conventionally divided into thin ultramicropores (less than 0.7 nm) and supermicropores that have an intermediate size between ultramicropore and mesopores.
In fact, all the pores in montmorillonite referred above of the size not larger than 6-9 nm for mesopores and 1-3 nm for micropores are predominantly involved in the adsorption [60].The average pore size measured in natural and modified montmorillonites is about 5 nm which corresponds to interparticle pores according to Osipov and Sokolov [52].At the same time, acid treatment, as shown above, leads to the appearance of pores in the structure of the layer (micropores) by partial leaching of octahedral cations, protonation of OH-groups, and changes of Al 3+ coordination.The above process does not lead to an increase of an average pore size, however, it results in an increase of the total pore volume (Table 1).
Mesopores of a size about 5 nm and a small amount of micro-pores with a diameter of about 3 nm are predominant in the natural montmorillonite.After treatment with hydrochloric acid with concentration up to a 3.0 M at room temperature for 7 days, significant changes in the structure of the pore space were not observed, which also can be seen below in the N2 adsorption-desorption isotherms.Treatment with 1 M HNO3 for 12 h does not lead to significant changes of the pore space either.The most significant changes were observed as a result of treatment for 108 h (Figure 6a): the Critical values for describing the concepts of "micropores", "mesopores", and "macropores" vary in classifications developed for different science approaches.In engineering geology, for example, mesopore sizes are in the range of 10 to 1000 µm and micropores are from 0.1 to 10 µm [56].V. Osipov and V. Sokolov in their morphometric investigations of soil microstructures with different composition [52] detailed the previously proposed classification of pore sizes.They divided micropores into thin (0.1-10 µm), small (1-10 µm), and large (10-100 µm) ones, suggesting the lower limit for macropores as 100 µm.
There are other classifications to consider.For example, the International Union for Pure and Applied Chemistry (the IUPAC) recommended to distinguish pores size into macropores (50 nm), mesopores (2 to 50 nm), and micropores (up to 2 nm) [57][58][59].The micropores are conventionally divided into thin ultramicropores (less than 0.7 nm) and supermicropores that have an intermediate size between ultramicropore and mesopores.
In fact, all the pores in montmorillonite referred above of the size not larger than 6-9 nm for mesopores and 1-3 nm for micropores are predominantly involved in the adsorption [60].The average pore size measured in natural and modified montmorillonites is about 5 nm which corresponds to interparticle pores according to Osipov and Sokolov [52].At the same time, acid treatment, as shown above, leads to the appearance of pores in the structure of the layer (micropores) by partial leaching of octahedral cations, protonation of OH-groups, and changes of Al 3+ coordination.The above process does not lead to an increase of an average pore size, however, it results in an increase of the total pore volume (Table 1).
Mesopores of a size about 5 nm and a small amount of micro-pores with a diameter of about 3 nm are predominant in the natural montmorillonite.After treatment with hydrochloric acid with concentration up to a 3.0 M at room temperature for 7 days, significant changes in the structure of the pore space were not observed, which also can be seen below in the N 2 adsorption-desorption isotherms.Treatment with 1 M HNO 3 for 12 h does not lead to significant changes of the pore space either.The most significant changes were observed as a result of treatment for 108 h (Figure 6a): the amount of 5-5.5 nm mesopores significantly increases and micropores with an average diameter of 3.5 nm appear which is not observed in natural montmorillonite samples.The particle size distribution changes even at low exposure times in the experiment with 1 M HNO 3 (Figure 6b) from monomodal shape with a maximum at 50 µm to a multimodal shape with peaks at ~80, 100, 145, and 200 µm.Thus, with increased micro-and mesoporosity, particle aggregation occurs that leads to the formation of relatively large aggregates of different sizes.
Minerals 2017, 7, 49 10 of 14 amount of 5-5.5 nm mesopores significantly increases and micropores with an average diameter of 3.5 nm appear which is not observed in natural montmorillonite samples.The particle size distribution changes even at low exposure times in the experiment with 1 M HNO3 (Figure 6b) from monomodal shape with a maximum at 50 μm to a multimodal shape with peaks at ~80, 100, 145, and 200 μm.Thus, with increased micro-and mesoporosity, particle aggregation occurs that leads to the formation of relatively large aggregates of different sizes.Adsorption-desorption isotherms of N2 are shown in Figure 6.As shown earlier, exposure to 0.25 and 0.5 M HCl for 7 days at room temperature results only in minor changes in the chemical composition (Table 2).The same tendency is observed in changes of N2 adsorption-desorption isotherms for samples Mt-M and Mt-T1 (Figure 6).Mt-T1 sample was treated only at 0.5 M HCl concentrations and is not shown in Figure 7 since the changes in the adsorption-desorption rates are within the error margin of the method.Hysteresis loop and the shape of the isotherm is close to the type IVa [61,62], which means that the natural and acid-modified montmorillonites studied can be characterized as mesoporous adsorbents where monolayer-multilayer adsorption is followed by capillary condensation.There is a significant amount of the micropores and mesopores so N2 adsorption/desorption isotherms cannot be described by one classical type only [61].
Hysteresis loops may have a different shape and, in the study of natural montmorillonite, can be attributed to the H3-H4 type [61].Loops of this type correspond to the interaction between nonrigid lamellar particles.Smooth slope loops correspond to filling the micro-and mesopores.Increase in steepness after the acid treatment may be explained by an increase of the pore volume and the appearance of macro-porosity.The specific hysteresis loop of the sample Mt-T2 isotherm Adsorption-desorption isotherms of N 2 are shown in Figure 6.As shown earlier, exposure to 0.25 and 0.5 M HCl for 7 days at room temperature results only in minor changes in the chemical composition (Table 2).The same tendency is observed in changes of N 2 adsorption-desorption isotherms for samples Mt-M and Mt-T1 (Figure 6).Mt-T1 sample was treated only at 0.5 M HCl concentrations and is not shown in Figure 7 since the changes in the adsorption-desorption rates are within the error margin of the method.
Minerals 2017, 7, 49 10 of 14 amount of 5-5.5 nm mesopores significantly increases and micropores with an average diameter of 3.5 nm appear which is not observed in natural montmorillonite samples.The particle size distribution changes even at low exposure times in the experiment with 1 M HNO3 (Figure 6b) from monomodal shape with a maximum at 50 μm to a multimodal shape with peaks at ~80, 100, 145, and 200 μm.Thus, with increased micro-and mesoporosity, particle aggregation occurs that leads to the formation of relatively large aggregates of different sizes.Adsorption-desorption isotherms of N2 are shown in Figure 6.As shown earlier, exposure to 0.25 and 0.5 M HCl for 7 days at room temperature results only in minor changes in the chemical composition (Table 2).The same tendency is observed in changes of N2 adsorption-desorption isotherms for samples Mt-M and Mt-T1 (Figure 6).Mt-T1 sample was treated only at 0.5 M HCl concentrations and is not shown in Figure 7 since the changes in the adsorption-desorption rates are within the error margin of the method.Hysteresis loop and the shape of the isotherm is close to the type IVa [61,62], which means that the natural and acid-modified montmorillonites studied can be characterized as mesoporous adsorbents where monolayer-multilayer adsorption is followed by capillary condensation.There is a significant amount of the micropores and mesopores so N2 adsorption/desorption isotherms cannot be described by one classical type only [61].
Hysteresis loops may have a different shape and, in the study of natural montmorillonite, can be attributed to the H3-H4 type [61].Loops of this type correspond to the interaction between nonrigid lamellar particles.Smooth slope loops correspond to filling the micro-and mesopores.Increase in steepness after the acid treatment may be explained by an increase of the pore volume and the appearance of macro-porosity.The specific hysteresis loop of the sample Mt-T2 isotherm Hysteresis loop and the shape of the isotherm is close to the type IVa [61,62], which means that the natural and acid-modified montmorillonites studied can be characterized as mesoporous adsorbents where monolayer-multilayer adsorption is followed by capillary condensation.There is a significant amount of the micropores and mesopores so N 2 adsorption/desorption isotherms cannot be described by one classical type only [61].
Hysteresis loops may have a different shape and, in the study of natural montmorillonite, can be attributed to the H3-H4 type [61].Loops of this type correspond to the interaction between nonrigid lamellar particles.Smooth slope loops correspond to filling the micro-and mesopores.
Increase in steepness after the acid treatment may be explained by an increase of the pore volume and the appearance of macro-porosity.The specific hysteresis loop of the sample Mt-T2 isotherm after prolonged exposure to 1 M HNO 3 (Figure 7b) can be attributed to the type H2(b) [61].This type of a desorption branch of the isotherm can be the result of a complex system of channel and pore formations and a partial blocking of them.
As a result of exposure to acid solutions, the hysteresis loop increases.In particular, it is noticeable after a prolonged influence of nitric acid at a high temperature (Figure 6b) when the hysteresis loop changes in a certain way.
The measured cation exchange capacity value (CEC) decreases from 86 meq/100 g in natural bentonite clay to 40 meq/100 g in bentonite samples with the longest time of the HNO 3 solutions treatment for 108 h (Table 1).Such a decrease of CEC under the treatment with inorganic acid solutions (e.g., HCl and H 2 SO 4 ) was described by other researchers [12].Cation exchange capacity of bentonite clays is connected not only to montmorillonite content but primarily, to such structural particularities as layer charge and its distribution among octahedral sheets.As a result, treatment with inorganic acid solutions leads to layer charge reduction due to leaching of the octahedral cations and the structural degradation; all of which leads to a decrease in the cation exchange capacity.In addition, amorphous silica appeared as a result of the destruction of tetrahedral sheets.The silica can settle on the surface of montmorillonite particles and reduce its exchange capacity.
A lot of attention in the work on acid treatment of natural bentonite clay was dedicated to the issues of activation-i.e., improving the properties of modified clays in comparison with natural clays [63,64].An increase of specific surface area and pore volume is observed in the course of the conducted experiments (Table 1).Leaching of the cations from interlayer space and octahedral positions results in a modification of layer charge and particles in general, this in turn affects the interaction of the individual particles between each other.As a result, the destruction of large aggregates, the restructuring of smaller ones, the appearance of an uneven surface, and the appearance of micropores on the site of octahedral grid take place.All the processes described above result in an increase of the total pore size and surface area.However, opportunities for cation exchange and its capacity are reduced due to layer charge modification and protonation of interlayer space.

Conclusions
The study concludes that an interlayer modification occurs even at early stages of treatment with HCl and HNO 3 solutions.This modification involves partial substitution of interlayer cations, especially Ca and Mg, to oxonium and partial protonation of the interlayer space.As the least stable components, natural nano-sized smectites and the most defective phase are completely dissolved in natural montmorillonite samples after acidic treatment.
Further treatment of montmorillonite structure with inorganic acid solutions (with increasing concentration and exposure period) leads to further modification of its structure accompanied by intense leaching of cations from the octahedral positions and partial penetration of leached octahedral cations into interlayer space.These transformations lead to partial protonation of OH-groups, partial destruction of the octahedral sheets, and modification of the interaction between tetrahedral and octahedral sheets which changes layer charge and the nature of interaction between adjacent layers and partial amorphization of tetrahedral sheets.A decrease in the layer charge leads to a decrease in CEC.
As a result of structural transformation, specific surface area increases.Also, volume and pore diameters increase as well as micropores of a 3.5 nm diameter occur which is not typical for natural montmorillonites.As a consequence, particle size distribution changes.Due to the rise in micro-and mesoporosity, adsorption capacity of the acid-treated bentonite clays still remains high.
Modification of structural and adsorption characteristics with the acid treatment can be used to simulate behavior of the engineered barrier properties for repositories of radioactive and industrial wastes, especially in the case of dealing with liquid radioactive wastes.
Figure 2c.It corresponds to Al-Al-OH and Al-Fe-OH vibrations in the structure of octahedral sheet of montmorillonite (Mt-T2 sample) during the treatment with the nitric acid.It may indicate a leaching of Fe 3+ and Al 3+ from the octahedral sites.[44-49].Reduction of the band intensities at 925 and 876 cm −1 is shown at Figure 2c.It corresponds to Al-Al-OH and Al-Fe-OH vibrations in the structure of octahedral sheet of montmorillonite (Mt-T2 sample) during the treatment with the nitric acid.It may indicate a leaching of Fe 3+ and Al 3+ from the octahedral sites.

Figure 2 .
Figure 2. Areas of interest on MM-T IR spectra before and after treatment with 1 M HNO3 60 °C: (a,b) the characteristic part of spectrum; (c) a magnified part of the spectra.

Figure 2 .
Figure 2. Areas of interest on MM-T IR spectra before and after treatment with 1 M HNO 3 60 • C: (a,b) the characteristic part of spectrum; (c) a magnified part of the spectra.

Figure 3 .
Figure 3. Transmission electron microscopic (TEM) images: (a) natural particles from the sample MM-T; (b) treated with 0.25 M HCl; (c) natural sample MM-M.

Figure 4 .
Figure 4. Transmission electron microscopic (TEM) images of natural montmorillonite particles (a,b), and treated with 1 M HNO3 for 108 h (c,d).Microdiffraction figures are located at enlarged areas.(b,d) high resolution images from the folded edge of the montmorillonite particles (area locations are shown as black rectangles in Figure (a,c), respectively).

Figure 3 .
Figure 3. Transmission electron microscopic (TEM) images: (a) natural particles from the sample MM-T; (b) treated with 0.25 M HCl; (c) natural sample MM-M.

Figure 3 .
Figure 3. Transmission electron microscopic (TEM) images: (a) natural particles from the sample MM-T; (b) treated with 0.25 M HCl; (c) natural sample MM-M.

Figure 4 .
Figure 4. Transmission electron microscopic (TEM) images of natural montmorillonite particles (a,b), and treated with 1 M HNO3 for 108 h (c,d).Microdiffraction figures are located at enlarged areas.(b,d) high resolution images from the folded edge of the montmorillonite particles (area locations are shown as black rectangles in Figure (a,c), respectively).

Figure 4 .
Figure 4. Transmission electron microscopic (TEM) images of natural montmorillonite particles (a,b), and treated with 1 M HNO 3 for 108 h (c,d).Microdiffraction figures are located at enlarged areas.(b,d) high resolution images from the folded edge of the montmorillonite particles (area locations are shown as black rectangles in Figure (a,c), respectively).

Figure 5 .
Figure 5. Schematic picture representing the structural changes in natural montmorillonite under the treatment with inorganic acid solutions: (a) natural Ca-montmorillonite; (b) partial protonation of interlayer space; (c) full protonation of interlayer space (H-smectite) and protonation of the OH-groups of the octahedral sheet and Al coordination change.

Figure 5 .
Figure 5. Schematic picture representing the structural changes in natural montmorillonite under the treatment with inorganic acid solutions: (a) natural Ca-montmorillonite; (b) partial protonation of interlayer space; (c) full protonation of interlayer space (H-smectite) and protonation of the OH-groups of the octahedral sheet and Al coordination change.

Figure 6 .
Figure 6.Changes of the porosity (a) and the particle size distribution (b) of Mt-T2 a sample due to exposure to 1 M HNO3 at 60 °C.

Figure 6 .
Figure 6.Changes of the porosity (a) and the particle size distribution (b) of Mt-T2 a sample due to exposure to 1 M HNO 3 at 60 • C.

Figure 6 .
Figure 6.Changes of the porosity (a) and the particle size distribution (b) of Mt-T2 a sample due to exposure to 1 M HNO3 at 60 °C.

Table 1 .
Changes of the structural parameters, textural and adsorption characteristics of montmorillonites due to acid activation (Specific Surface Area: S BET , Total Pore Value: V Σ , Cation Exchange Capacity: CEC).

Table 2 .
Chemical composition of MM-T2 natural and modified by 1 M HNO 3 at 60 • C.