Regio- and Stereospecific Analysis of Triacylglycerols—A Brief Overview of the Challenges and the Achievements

The efforts to reveal, in detail, the molecular and intramolecular structures of one of the main lipid classes, namely, triacyl-sn-glycerols, which are now known to affect their specific and important role in all living organisms, are briefly overviewed. Some milestones of significance in the gradual but continuous development and improvement of the analytical methodology to identify the triacylglycerol regio- and stereoisomers in complex lipid samples are traced throughout the years: the use of chromatography based on different separation principles; the improvements in the chromatographic technique; the development and use of different detection techniques; the attempts to simplify and automatize the analysis without losing the accuracy of identification. The spectacular recent achievements of two- and multidimensional methods used as tools in lipidomics are presented.


Introduction
Triacylglycerols (TAGs further in the text), or triglycerides (as they were denoted previously), or triacyl-sn-glycerols (as they are denoted more correctly at present), consist of a glycerol moiety in which the three hydroxyl groups (-OH further in the text) are esterified with fatty acids (FAs). Triacylglycerols are the main and most abundant class of simple lipids, denoted as simple because they yield two types of primary products per molecule in hydrolysis: glycerol and fatty acids [1]. The need to know, in detail, the TAG molecular and intramolecular structures is determined by their specific role in the life of plants, animals and humans: (i) TAGs are the main component of natural oils and fats of plant and animal origin, including all edible oils [1]; (ii) TAGs are one of the main energy sources for living organisms [2]; (iii) TAGs are the most important source of FAs that are essential for human health [3]; (iv) the intramolecular structure of TAGs strongly influences both the digestibility and metabolism of dietary FAs [4]. On the other hand, the TAG composition of plants is impacted by cultivar, harvest period and geographical origin [5][6][7][8], while that of animals depends on feeding as well [9,10]. Thus, the detailed examination of the TAG molecular structure has always been one of the main tasks of lipid analysis. The problem that the analysis faces is that since natural TAGs are produced by enzymatic synthesis, a center of asymmetry exists at position 2 [11] (Figure 1), while, in addition, each of the three -OH groups might be esterified by a different FA. Indeed, in most fats and oils, FAs are usually limited to the 20 most abundant in nature (out of the approximately 1000 described as yet [11]), but even these are of different chain lengths, might have different numbers, positions and configurations of double bonds and may esterify any of the -OH groups, leading to the formation of a spectacular number of TAG species in many natural lipids (consider milk fat, fish oil, other marine organisms or bacteria, for example). Thus, depending on the FA structure and position on the glycerol backbone, the simple lipid class of TAGs presents a number of different structures, including regioisomers and/or enantiomers, creating a serious challenge for efforts aimed at determining their molecular and intramolecular structures. in the regio-and stereospecific analysis of TAGs while not forgetti been discussed since the very early stage of lipid analysis in the tury, and that the principles used now in approaches denoted as t dimensional, orthogonal, etc., were developed and suggested in th

Regiospecific Analysis
The sn-TAGs that differ in the FA composition between the or between the sn-1 (3) and sn-2 positions, are denoted as regioiso tion was the first, and probably the easiest, step in the examination structures. The large number of analytical methods developed thus two major groups in general: (i) indirect approaches, i.e., metho degradation step by either enzymatic hydrolysis or with a Grigna nesium bromide), followed by isolation of partial acylglycerols (all acylglycerols) by a normal-phase chromatographic procedure, sil matography (TLC), silica gel column (CC) or solid phase extraction chromatographic determination of the FAs and reconstructing the approaches (conditional), i.e., chromatographic, mass spectrometr netic resonance (NMR) procedures based on certain specific chara ular composition of the regioisomeric TAG. Note, however, that M The interest in lipid analysis, and that of FAs and TAGs in particular, has a very long history, but the most important achievements were made in the middle of the 20th century [12].
Step by step, using the power of any new technique for separation, detection, identification and quantification that appeared resulted in the development of a number of analytical approaches in TAG analysis, allowing for examining, in more detail, their molecular and intramolecular structures [13][14][15][16][17][18][19] in efforts: (i) to obtain information that is as complete as possible on the TAG composition of natural oils and fats of plant/animal origin and better understand the pathways of TAG enzymatic synthesis and their role in biological systems; (ii) to expose and better understand TAGs' role in living organisms, including human health and diet; (iii) to produce special formulations, such as infant milk-like products, food additives and others; (iv) to control the process and the quality of edible oils and fats and unequivocally trace adulteration.
Looking back, it seems that the leading motivation in developing new approaches was to increase the efficiency of the resolution and, mostly, to simplify the rather complicated TAG analysis as much as possible by cutting down as many analytical stages as possible and automatizing the analytical procedure. However, at present, one still has to examine, in detail, the FA composition as the first stage of a reliable determination of TAG molecular/intramolecular structures. Having this in mind, here, we briefly review the achievements of the analytical approaches for regio-and stereospecific analysis of TAGs.
The references cited here are only part of the papers dedicated to the topic, and the review has no pretention to be comprehensive. Efforts were made to show the milestones in the regio-and stereospecific analysis of TAGs while not forgetting that the problem has been discussed since the very early stage of lipid analysis in the middle of the 20th century, and that the principles used now in approaches denoted as two-dimensional, multidimensional, orthogonal, etc., were developed and suggested in those days.

Regiospecific Analysis
The sn-TAGs that differ in the FA composition between the sn-1 and sn-2 positions, or between the sn-1(3) and sn-2 positions, are denoted as regioisomers. Their determination was the first, and probably the easiest, step in the examination of TAG intramolecular structures. The large number of analytical methods developed thus far can be divided into two major groups in general: (i) indirect approaches, i.e., methods using a preliminary degradation step by either enzymatic hydrolysis or with a Grignard reagent (ethylmagnesium bromide), followed by isolation of partial acylglycerols (all possible di-and mono-acylglycerols) by a normal-phase chromatographic procedure, silica gel thin layer chromatography (TLC), silica gel column (CC) or solid phase extraction (SPE), followed by gas chromatographic determination of the FAs and reconstructing the regioisomers; (ii) direct approaches (conditional), i.e., chromatographic, mass spectrometric (MS) or nuclear magnetic resonance (NMR) procedures based on certain specific characteristics of the molecular composition of the regioisomeric TAG. Note, however, that MS and NMR are indirect methods requiring reconstruction of the initial molecule based on a specific instrumental response.

Enzymatic Hydrolysis
Historically, this was the first approach to provide information on regioisomeric TAGs, and the method was published as early as 1965 by Brockerhoff [20,21]. Pig pancreatic lipase was used since it appeared to be specific to hydrolyze the ester bonds at positions sn-1 and -3 in the molecule, producing a 2-monoacylglycerol whose FA composition was then easily determined. This procedure is multi-staged and has certain limitations due to the rate of hydrolyzation of the FAs. It works well with TAGs of common plant or animal origin since all straight-chain saturated FAs are hydrolyzed by the same rate irrespective of the chain length. Short-chain saturated FAs, such as those in milk TAGs (such as butyric acid), however, hydrolyze more rapidly, while long-chain polyunsaturated FAs (such as those in fish oil or marine lipids) hydrolyze more slowly. Many variations of this approach have been developed since the one suggested by Brockerhoff in 1965, but at the core, the procedure is, in general, the same as that shown by Christie [22]. The procedure that is most frequently used is described in detail in [23]. Other lipases have been used for the same purpose such as Rhizopus arrhizus (family Mucoraceae), but they have not shown any substantial advantages thus far [1].

Chemical Methods
Hydrolysis with a Grignard reagent-ethyl magnesium bromide [24] or allyl magnesium bromide [25]-is also a multi-stage procedure. Here, it is essential to stop the reaction before completion, which is advisable at 50% hydrolysis. TLC on silica gel G plates, impregnated with boric acid, is applied to isolate the various partial sn-acylglycerols: 1,2-, 2,3-and 1,3-diacyl-sn-glycerols (DAGs), and 1-, 2-and 3-monoacyl-sn-glycerols (MAGs), followed by isolation, determination of FAs and calculations to determine their position in the glycerol backbone (for example, [26] presented details of one of the published analytical protocols).
Grignard hydrolysis has been used as a first step in many other procedures aiming to determine the regioisomeric TAGs [27,28].

Chromatography
Chromatography is an analytical tool that advanced lipid chemistry and led to an incomparable better understanding of the very complex mixture of natural TAGs. Performed in different forms: as a layer or column of a silica adsorbent pre-treated with different reagents in order to modify or change the functionality and increase the selectivity of the separation power, combined with new effective methods of detection, automatized and computerized, this technique allowed for looking inside the TAG molecular and, later, intramolecular structure. All chromatographic techniques developed since the middle of the 20th century have been applied in TAG analysis, and many allow for the determination of their composition in a large number of natural plant and animal lipids of terrestrial and marine origin. Of these, silver ion and reversed-phase liquid chromatography techniques were, and still are, the most successfully used, and the procedures developed thus far easily resolve and unambiguously identify TAG regioisomers, i.e., determination of these isomers is no longer a problem for lipid chemists of common oils and fats [17,19].

Silver Ion Thin-Layer Chromatography (Ag-TLC)
A significant amount of research has been reported and discussed regarding the chemical processes that lay the fundament of this technique [29][30][31][32][33], which is forgotten or rarely used now, not because it provides less reliable results for common fats and oils, but mostly because it requires more laborious laboratory work in order to compensate for the much lower efficiency of the TAG resolution. Briefly, the separation of TAGs is based on a distinctive property of unsaturated organic compounds-FA moieties in this case-to form weak reversable charge-transferred complexes with silver ion, Ag + [33,34]. Thus, Ag + chromatography allows resolving TAGs in the order of general unsaturation, with those that are most saturated migrating first. In addition, it is possible to easily differentiate between such regioisomeric pairs as SMS/SSM, SMM/MSM, SDS/SSD, MDM/MMD, Symmetry 2022, 14, 247 4 of 16 SDD/DSD and MDD/DMD (S, M and D stand for saturated, monoenoic and dienoic FA moieties; in each pair, the first TAG migrates ahead) [29,30,[34][35][36]. The technique consists of the application of the TAG sample as a band on an impregnated plate, which, in turn, is developed in a closed or open tank with a defined volume of the mobile phase. If required, the plate is dried, and the development is repeated with the same or another mobile phase. It is also possible to apply the sample on two or more different plates, developing each separately with different mobile phases to obtain the desired resolution. Details of the analytical procedures can be found in [30,32,37].
Historically, the first achievement in the separation of regioisomeric TAGs was demonstrated by Blank et al. as early as 1965 [35], i.e., three years after silver ion chromatography, both as the thin layer and column techniques, was introduced for the first time in the practice of lipid analysis, allowing for separation of TAG molecular species. In both cases, silica gel G, used as a carrier, was impregnated with 0.5-10% AgNO 3 in methanol, and mixtures of chloroform-methanol and n-hexane (petroleum ether)-acetone of different proportions (to name only the most frequently used) were employed as mobile phases. Procedures to quantify the TAG composition by different direct and indirect approaches have been developed, with densitometric scanning of the plate providing accurate results concerning the relative content of each TAG class [30]. The qualitative and quantitative results were usually confirmed by gas chromatographic determination of the respective FA composition. It should be stressed that a large number of common fats and oils of different origin, as well as TAGs in plant and animal tissues and organs, have been studied by employing this tedious, time-consuming technique, and the information collected, while not being as detailed as that provided by later techniques, is undeniably correct. By using Ag-TLC to resolve and identify regioisomeric TAGs, it has been possible to detect adulteration in cocoa butter [38] and olive oil [39] (note the publication years). More information about these early techniques, including quantification approaches, can be found in [1,30,31,33].

Silver Ion High-Performance Liquid Chromatography (Ag-HPLC)
Once Christie [40] published the simple effective procedure to load AgNO 3 on a commercial HPLC Nucleosil silica gel column, Ag-TLC was almost completely replaced by Ag-HPLC in studying the TAG composition of lipids. Among the other advantages, using HPLC allowed connecting columns in series [41] and applying gradient elution, thus significantly increasing the efficiency of the TAG resolution and clearly resolving species such as various regioisomers [41][42][43][44][45]. The published procedures used 10% AgNO 3 to load the column, and a complex solvent gradient of either acetonitrile [41,42] or ethylacetate [44,46] in n-hexane [41,42,46] or toluene [43,44] for resolution of regioisomeric TAGs in relatively simple samples such as cocoa butter or palm oil. Later, it was possible to work with commercial silver-loaded ChromSpher Lipids columns. In addition, it was technically possible to connect the column to one or more detectors, working on different principles: ultraviolet (UV), refractive index (RI), flame ionization (FID), evaporative light scattering (ELSD), and especially series of mass detectors: electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), thus gaining more information in a single run [1,15,[32][33][34]37,45,47,48]. The analytical protocols presented in these references describe, in detail, all analytical stages. The pairs SMS/SSM, SDS/SSD, SMM/MSM and SDM/SMD/MSD were resolved (i.e., the same pairs as those in Ag-TLC; notation of FA moieties is the same as above). To the best of the authors' knowledge, thus far, only Adlof [41,42], using two home-made columns connected in series, isocratic elution and UV detection, and Holcapek et al. [45,49], using three ChromSpher Lipids columns connected in series, gradient elution and an APCI-MS detector, were able to resolve and identify regioisomeric TAG pairs containing trienoic FAs. Thus, the resolution of the pairs SOS/SSO, SOO/OSO, SLnS/SSLn, SLnO/SOLn/OSLn, OLnO/OOLn, SLnLn/LnSLn and OLnLn/LnOLn (S, saturated; O, oleic; Ln, linolenic acid moieties) was demonstrated [49].
The FID, ELSD and APCI/ESI detection techniques allow for semiquantitative estimation of the relative TAG content in the sample. For a correct quantification, the detector should be calibrated against an external or internal standard, the latter requiring both a generic relation to and perfect separation from the components in the sample [50].
At this stage, it appears that Ag-HPLC while providing almost the same resolution of regioisomeric TAGs outstages Ag-TLC in achieving the results in shorter elution time with by far higher accuracy and precision. The disadvantage of Ag-HPLC is the tedious work to prepare a home-made silver-loaded column and the relatively short life (several months) of the commercial columns.

Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC)
For many reasons, most of which are out of the scope of this review, another separation technique, which was firstly developed in TLC mode as well, has gained very wide and dominating application in TAG analysis-high-performance liquid chromatography, which uses columns packed with silica gel particles of different sizes with bonded octyl-(C8) or octadecylsilane (ODS or C18 columns) moieties and mixtures of polar solvents as a mobile phase. This technique is denoted as either reversed-phase liquid chromatography, RP-HPLC, or, more correctly in the case of TAG separation, non-aqueous reversed-phase liquid chromatography (NARP-LC). In brief, TAGs are separated according to their equivalent carbon number (ECN) which represents their general polarity-a combination of the number of carbon atoms (NCA) and the number of double bonds (NDB), i.e., ECN = NCA-2NDB, in the acyl chains, with a TAG retained as strongly as higher is the respective ECN (i.e., the higher the number of carbons, the lower the number of double bonds in the acyl chains) [13,19,[51][52][53][54][55]. Moreover, the book chapter [52] and the literature cited therein presented some aspects of the mechanism of retention and separation in RP-HPLC of TAGs. The efficient combination of a complex elution gradient with mobile phases based on (mainly) acetonitrile modified with dichloromethane, 2-propanol or n-hexane (as a stand-alone solvent or in mixtures) with RI, ELSD or a mass detector (positive/negative CI, ESI and APCI [1,19]) allowed for clear differentiation of TAGs according to the chain length of FA moieties and the position and configuration of double bonds in the chains and definitely allowed gaining significantly more information on the TAG composition in natural oils and fats. Evidence was found that the FA moieties at position 2 might affect the resolution of regioisomers [56], and, indeed, a complete simultaneous regioselective separation of ten isomeric triacylglycerols in a single isocratic run on a single C18 column was demonstrated [57]. Separation and determination of isomeric TAGs in natural TAG samples, however, have not been achieved, and it seems that the efforts to further explore this possibility were abandoned. As above, detection with ELSD and MS allows for estimating only the relative quantity of the components.
Later, it was demonstrated that an indirect method based on the specific fragmentation pattern of TAG species in mass spectrometry might be very fruitful in identifying and quantifying TAGs in general and regioisomeric TAGs in particular. It appeared possible to identify regioisomers by using mass detectors and reconstructing their structure by analyzing the fragmentation in their mass spectra. This approach was based on the relative intensities of the [M-RCOO] + ions which were shown to depend on the position of FA moieties in the molecule [58][59][60]. More information on the principles and application is presented in [61][62][63]. APCI is most frequently used for the purpose, and TAG regioisomers in a large number of plant oils [60,[64][65][66][67], algae [68,69], fish oils [70], bacteria [71][72][73], yeasts [74] and insects [75] have been identified. The importance of the chromatographic resolution to correctly identify the TAG composition was understood, and efforts were directed to improving the reconstruction, by improving the chromatographic step either by testing the properties of the stationary phase [57] or by elongating the chromatographic "path" through connecting two columns in series [76,77].
The efficiency of ESI in TAG analysis has been discussed in detail in [1] (part 5), including the efficiency in the quantification of molecular species. ESI was used to examine the retention behavior of TAGs in RP-HPLC [78], to determine regioisomeric TAGs containing branched-chain FAs (tandem positive ESI) [79] and for identification of TAG regioisomers in lard, tallow, yolk, chicken skin, palm oil, palm olein, palm stearin and trans-esterified blends of palm stearin and coconut (tandem negative ESI) [80], in milk [81] and in fish oil (triple-stage ESI) [82].
Quantification of TAG species in RP-HPLC/MS has been thoroughly discussed, and the need for careful calibration has been clearly exposed [64,76,77,83].

Combined Chromatographic Methods
As discussed above, even the simple natural oils and fats, where TAGs are the main lipid component and the FA composition is limited to 4 or 5 straight-chain FAs with 16 to 18 carbon atoms and 0 to 3 double bonds, might form a large number of molecular species, presenting problems for their identification and quantification. The problems increase when it comes to studying TAGs in more complicated natural sources such as milk fat and fish oils in marine organisms and are further multiplied when a detailed TAG analysis is engaged in lipidomics [1]. To answer these challenges, lipid analysis turned back to the well-exploited combination of normal-phase, silver ion and non-aqueous reversed-phase high-performance liquid chromatography, i.e., it went back to the classical approach of successive application of separation techniques based on different separation principles. The successive separation stages simplified the TAG mixture to fractions with preliminary defined molecular characteristics (such as the unsaturation level and polarity) for further high-resolution mass detection, thus allowing for a better reconstruction of their intramolecular structure and quantification. At present, the online or offline connection of two or more chromatographic columns, which should be "orthogonal" (i.e., should use different separation principles such as Ag-and NARP-LC), is known as two-dimensional chromatography or is denoted as multidimensional chromatography in the case where additional preliminary steps are employed [84][85][86][87][88]. Note that the principles and opportunities of two-dimensional chromatography were presented nearly a decade ago [89,90]. Combining chromatography with enzymatic degradation and efficient mass detection allowed determining TAG regioisomers including those in complex and very complex samples, such as rice oil [91] and bacteria [79,92]. In most of the recent works where two-or multidimensional orthogonal chromatographic approaches and powerful high-resolution mass detection were used, the determination of regioisomers was part of the detailed stereospecific analysis of TAGs in lipids; examples are shown below.

Other
Besides the most widely used approaches shown above, occasionally other meth-  [17]. Supercritical fluid chromatography (SFC) has also been explored [93][94][95][96], and it is claimed that when coupled with mass detectors, SFC ensures a good identification of regioisomeric TAGs separated for shorter times than with HPLC under identical conditions. Thus, sn-POO/OPO/OOP were separated by SFC coupled with a triple quadrupole mass spectrometer (SFC/MS/MS) using a CHIRALPAK ® IG-U column with acetonitrile and methanol in the mobile phase (for example, [97]) for only 40 min, while 50 min was required for the resolution of PPLn/PLnP, PPL/PLP, PPO/POP, SPLn/SLnP, SPO/SOP and SSO/SOS pairs in palm and canola oils by SFC-positive ESI [98]. Mass spectrometry has been used in combination with other techniques besides chromatography. In fact, MS was applied as a single method for TAG analysis as early as the 1960 when Ryhage and Stenhagen observed that loss of acyloxymethylene in mass spectra occurred from positions 1 and 3 and not from position 2 of the glycerol moiety, which allowed for recognizing regioisomers [99]. The authors noted that further experimental work was necessary before such assignments could be made with any degree of confidence. Over the years, this observation was confirmed for all MS techniques. Among the most frequently used modes is ammonia-negative ion chemical ionization (NICI) tandem MS developed in the early 1990s by Kallio and Currie [100,101] and gradually optimized for application in analysis of TAG regioisomers in various samples [102][103][104][105][106]. On the other hand, positive electrospray ionization (ESI) tandem MS with cationization by lithium ions has been applied in several laboratories for the same purpose with both pure standards [107,108] and TAG fractions isolated from plant oils [109][110][111][112][113]. Atmospheric pressure chemical ionization (APCI) MS is another common technique for direct TAG analysis [59,114]. Byrdwell contributed to the method development by introducing the critical ratios for providing direct structural information for isomeric TAGs, and by creating a compact library of TAG mass spectra [115][116][117]. Two comprehensive reviews summarized the results of the sole use of mass spectrometry to resolve intramolecular TAG structures [17,118], and, indeed, direct MS was applied successfully in some cases such as in milk [119] and salmon fish [120]. An approach to determine the ratio of regioisomeric TAGs by direct ESI analysis was suggested in [121].

Nuclear Magnetic Resonance Spectrometry (NMR)
The efforts to use 13 C NMR for TAG analysis can be traced back to the 1990s [122,123], and NMR has been applied to identify the intramolecular structure of TAGs in olive oil [124] and tissues [125]. It seems that NMR is especially productive in studying milk TAGs and in controlling the production quality of structured lipids in infant formula through the ability to recognize the presence of certain TAG isomers [126,127]. The utilization and achievement of this technique were recently reviewed by Indelicato et al. [19].
Separate attention should be paid to the use of high-resolution techniques such as mass spectrometry and nuclear magnetic resonance spectrometry to directly determine the intramolecular TAG structure in biological samples, omitting the separation step. This approach currently denoted as "shotgun lipidomics" is very attractive. Undoubtedly, its used for rapid detection, for example, of regioisomers-reporting the adulteration of edible or expensive natural fats and oils, such as olive oil [128]-should be welcome. The use of MS in the shotgun approach was recently reviewed [129,130]. Estimating whether the shotgun procedures are able to provide full and correct results for the TAG intramolecular structure in all varieties of samples, irrespective of the complexity of their composition, is a matter of the future.

Stereospecific Analysis
Stereospecific analysis of TAGs aims to determine the FA moieties in each position (sn-1, -2, -3) in the glycerol backbone, i.e., to present, in full, the real intramolecular structure of each TAG in the lipid sample. As discussed above (Sections 2.1 and 2.2), the classical enzymatic [20] or Grignard procedures to partially hydrolyze TAGs, followed by series of derivatization and separation steps of the produced partial acylglycerols, allow for determining the intramolecular sn-1, -2 and -3 structures of species. As shown, these procedures have certain limitations due to the structure of the FA moieties, and, in addition, they are multi-staged, laborious and time consuming and require well-skilled staff. The further development of HPLC techniques and the introduction of chiral columns in the analytical practice have allowed for alternative approaches: (i) separation on achiral stationary phases of sn-TAGs via diastereomeric diacylglycerol derivatives, and (ii) resolution on chiral stationary phases of diacylglycerol or monoacylglycerol derivatives and, lately, of intact TAGs.
Inevitably, efforts were next focused on simplifying the analysis, and the obvious step was to search for conditions to resolve intact TAGs by chiral HPLC. Thus, various chiral stationary phases were developed along with wide application of two-or multidimensional approaches as described above-stepwise simplification of TAG mixtures by application of silver ion or reversed-phase HPLC as stand-alone or successive separation techniques. The key role in these approaches was played by high-resolution tandem MS for detecting and identifying TAG isomers. Ten years ago, Nagai and co-workers [146] tested several chiral columns (CHIRALPAK IA, IB, IC, AD-RH, AS-RH, AY-H, CHIRALCEL OD-RH, OJ-RH and OZ-H) based on polysaccharide derivatives coated or immobilized on silica gel for normalphase or reversed-phase HPLC. Promising results were achieved with the CHIRALCEL OD-RH column which could resolve racemic mixtures of asymmetric TAGs, containing palmitic, oleic and linoleic acyl residues. This new chiral separation method in combination with APCI-MS was capable of determining the ratio of sn-OOP to sn-POO in palm oil, and this was the first report of the separation of enantiomeric asymmetric natural TAGs. This analytical protocol was also applied for the analysis of TAGs in marine organisms [147] and bovine milk fat [148], and for the investigation of asymmetric TAGs [149]. Using the same system of recycling HPLC and connecting two chiral columns in series, Kalpio and co-workers separated enantiopure reference compounds of eleven racemic TAGs containing C12-C22 FAs with 0-2 double bonds [150], or omega-3 polyunsaturated FAs [151], and determined the isomers in sea buckthorn as an example food matrix [88]. Nagai and co-workers, using a CHIRALPAK IF-3 column, examined the separation of various TAG isomers and recommended this variant of chiral column as especially applicable for the analysis of the TAG composition of semi-solid fats such as palm oil [152,153]. A variation of this chiral phase (CHIRALPAK IG-U column) was applied by Masuda et al. [97] for the first report of simultaneous and rapid analysis of TAG regioisomers and enantiomers using SFC. The method was used to quantify sn-OPO, sn-POO and sn-OOP in extra virgin olive oil, refined olive oil, palm oil, palm olein and interesterified palm olein. Two Lux Cellulose-1 columns connected in series and combined with an APCI-MS detector allowed for the systematic investigation of TAG enantiomers in real samples of hazelnut oil and human plasma [154]. Thus, it was possible to determine all TAG enantiomers with acyl chains of different lengths and one to eight double bonds, except the TAG species with saturated, dienoic and/or trienoic FA moieties at the sn-1 and sn-3 positions. Applying the two-or multidimensional approach, it appeared possible to obtain more accurate information for TAGs with more complicated structures and in more complicated lipids. Chen et al. [155] determined isomeric TAGs in human milk by preliminary fractionation of the TAGs by RP-and Ag-HPLC followed by separation on the same Lux Cellulose-1 column. In their work studying TAG isomers in complex lipids, Rezanka and co-workers employed two Astec CYCLOBOND™ I 2000 3,5-dimethylphenyl carbamate-modified β-cyclodextrin chiral columns combined with high-resolution MS detection. TAGs with FA moieties of various types were first fractionated by the RP-HPLC stand-alone method, or combined with silver ion chromatography. Using this multidimensional approach, the research team was able to identify both regio-and stereoisomeric TAGs in diatom Phaeodactylum tricornutum (family Phaeodactylaceae) [72], in different Stichococcus strains (family Prasiolaceae) [73], in yeasts of seven genera [74], in nine cultivated strains of microalgae from different taxonomic groups [156], in protozoan and mold [157] and in the haptophyte alga Coccolithophora sp. [158], as well as TAGs with very long chain fatty acids [159], with branched fatty acids (iso and/or anteiso) [79], with cyclofatty acids [160] or with allenic and acetylenic fatty acids [161].
The scheme in Figure 2 [162] illustrates the essence of the multidimensional analytical protocol which includes a detailed examination of the FA composition, and steps to reveal, as fully as possible, the TAG molecular and intramolecular structures. Evidently, this approach is the one that, at present, provides the information needed for better understanding the role of the TAG structure (i.e., the exact position of FA moieties in the glycerol backbone) in the metabolism pathways in biological systems [163].
bined with an APCI-MS detector allowed for the systematic investigation of TAG enantiomers in real samples of hazelnut oil and human plasma [154]. Thus, it was possible to determine all TAG enantiomers with acyl chains of different lengths and one to eight double bonds, except the TAG species with saturated, dienoic and/or trienoic FA moieties at the sn-1 and sn-3 positions. Applying the two-or multidimensional approach, it appeared possible to obtain more accurate information for TAGs with more complicated structures and in more complicated lipids. Chen et al. [155] determined isomeric TAGs in human milk by preliminary fractionation of the TAGs by RP-and Ag-HPLC followed by separation on the same Lux Cellulose-1 column. In their work studying TAG isomers in complex lipids, Rezanka and co-workers employed two Astec CYCLOBOND™ I 2000 3,5-dimethylphenyl carbamate-modified β-cyclodextrin chiral columns combined with high-resolution MS detection. TAGs with FA moieties of various types were first fractionated by the RP-HPLC stand-alone method, or combined with silver ion chromatography. Using this multidimensional approach, the research team was able to identify both regio-and stereoisomeric TAGs in diatom Phaeodactylum tricornutum (family Phaeodactylaceae) [72], in different Stichococcus strains (family Prasiolaceae) [73], in yeasts of seven genera [74], in nine cultivated strains of microalgae from different taxonomic groups [156], in protozoan and mold [157] and in the haptophyte alga Coccolithophora sp. [158], as well as TAGs with very long chain fatty acids [159], with branched fatty acids (iso and/or anteiso) [79], with cyclofatty acids [160] or with allenic and acetylenic fatty acids [161].
The scheme in Figure 2 [162] illustrates the essence of the multidimensional analytical protocol which includes a detailed examination of the FA composition, and steps to reveal, as fully as possible, the TAG molecular and intramolecular structures. Evidently, this approach is the one that, at present, provides the information needed for better understanding the role of the TAG structure (i.e., the exact position of FA moieties in the glycerol backbone) in the metabolism pathways in biological systems [163].

Concluding Remarks
Efforts were made in this brief review to present the development in the analysis of TAGs in the last 70 years in pursuing the aim to determine, as fully as possible, their structure. The contributions of all widely used separation and identification techniques such as chromatography, mass spectrometry and nuclear magnetic resonance were presented, followed by their application, and some of their best achievements were explored. This review shows the readiness of the lipid community throughout the years to use all meth-

Concluding Remarks
Efforts were made in this brief review to present the development in the analysis of TAGs in the last 70 years in pursuing the aim to determine, as fully as possible, their structure. The contributions of all widely used separation and identification techniques such as chromatography, mass spectrometry and nuclear magnetic resonance were presented, followed by their application, and some of their best achievements were explored. This review shows the readiness of the lipid community throughout the years to use all methodological novelties and all improvements to reveal, as fully as possible, the TAG molecular and intramolecular structures of plant and animal origin, thus helping to better understand their important role in living organisms.