Plastic Debris in the Stomach of the Invasive Signal Crayfish Pacifastacus leniusculus from the Baltic Coastal River

: The signal crayfish Pacifastacus leniusculus is one of the most widespread non-indigenous crayfish in Europe and is of interest to aquaculture in many countries. Once they escape into the wild, they disperse and become a potential source of food exploited for consumption by local inhabitants. The ingestion of plastics by the invasive signal crayfish in the Wieprza River, a tributary to the Baltic Sea, was determined by the FTIR identification of plastic compounds found in stomachs. The occurrence of plastic debris in the stomachs of P. leniusculus is reported for the first time. Plastic particles with the size range between 70 and 450 µ m were observed only among crayfish inhabiting the lower, urbanized part of the river (7.3% of specimens). The presence of PTFE (polytetrafluoroethylene), cellophane, PP (polypropylene), PE (polyethylene), PMMA (polymethyl methacrylate), and nylon was confirmed with the highest frequency of PTFE in the stomachs of crayfish. Fibres were the predominant type of microplastics in crayfish stomachs. The results indicated the size-selective uptake and ingestion of plastics depending on the traits of the species and environment.


Introduction
Pollution by plastic debris, widely recognized as a growing global problem [1][2][3] is caused by the production of synthetic polymers.Over recent years, the production of plastics has increased, while in Europe, fossil-based plastics production has decreased, and recycled plastics production has increased [4].There are different types of plastics.Some types of plastic ingredients, applied in a wide variety of cosmetics and personal care products (PCCP), occur as particulate microplastics < 5 mm [5].Larger plastics, under environmental conditions, may fragment to microplastics [6].
Plastics are widely used by humans, and, as waste, they become a pollutant in the environment.Plastics may pose risks for aquatic ecosystems [7].Plastics are water-insoluble, have low degradation rates [8], may be ingested by various organisms ranging from zooplankton to fish and mammals, and accumulate in the intestines through the aquatic food web [9].Moreover, plastic can adsorb organic contaminants from the surrounding media, which can be transferred to organisms upon ingestion [10].
Most studies on plastic debris are limited to the marine environment [7,10,11].However, rivers are significant sources of plastic pollution for coastal and offshore areas [12,13].
The signal crayfish is one of the most widespread non-indigenous crayfish in Europe [19].They are large, euryhaline, attractive for consumption and for this reason were introduced for aquaculture into many countries [20,21].In the 1990s, this species was introduced into the Wieprza River drainage area [22,23].Once they escaped into the wild, they became exploited by local inhabitants.
The aim of this study was to determine the level of contamination by plastics in the invasive signal crayfish from the Baltic coastal tributary that is considered as a potential local food source.The specific objectives were: 1.
determination of the plastic contamination in different the river sections, 2.
verification if there are any differences between sexes, sizes and condition of organisms resulted from distinct traits of individuals.

Material Collection and Preparation
Specimens of P. leniusculus were collected from the Wieprza River and its tributary in the summer months between 2014 and 2018 (Figures 1 and 2).Crayfish were caught at two sites: in the upstream section of the River Studnica above Miastko (n = 25) and the downstream section of the Wieprza River which is close to Baltic Sea in Darłowo (Poland) (n = 205).Crustaceans were caught using the "Pirate" crayfish traps at both sites and by the hand collecting method to increase the sample size from Miastko [24].The distance between traps was between 5 and 20 m within the studied areas.After the capture, animals were immediately frozen (−20 • C).In the laboratory, thawed crayfish were sexed and measured for total length (L T ) with digital callipers.The wet mass (M W ) was determined using a Mettler Toledo XS 205 scale (Greifensee, Switzerland).The L T and M W were expressed as a mean with a standard deviation (mean ± SD).
The signal crayfish is one of the most widespread non-indigenous crayfish in Europe [19].It is large, euryhaline, and attractive for consumption and for this reason was introduced for aquaculture into many countries [20,21].In the 1990s, this species was introduced into the Wieprza River drainage area [22,23].Once it escaped into the wild, it dispersed and, in some stretches of the river, became exploited by local inhabitants.
The aim of this study was to determine the level of contamination by plastics in the invasive signal crayfish from the Baltic coastal tributary that is considered as a potential local food source.The specific objectives were: 1. determination of the plastic contamination in different the river sections, 2. assess microplastic amount and type, 3. verification if there are any differences between sexes, sizes and condition of organisms resulted from distinct traits of individuals.

Material Collection and Preparation
Specimens of P. leniusculus were collected from the Wieprza River and its tributary in the summer months between 2014 and 2018 (Figures 1 and 2).Crayfish were caught at two sites: in the upstream section of the River Studnica above Miastko (n = 25) and the downstream section of the Wieprza River which is close to Baltic Sea in Darłowo (Poland) (n = 205).Crustaceans were caught using the "Pirate" crayfish traps at both sites and by the hand collecting method to increase the sample size from Miastko [24].The distance between traps was between 5 and 20 m within the studied areas.After the capture, animals were immediately frozen (−20 °C).In the laboratory, thawed crayfish were sexed and measured for total length (LT) with digital callipers.The wet mass (MW) was determined using a Mettler Toledo XS 205 scale (Greifensee, Switzerland).The LT and MW were expressed as a mean with a standard deviation (mean ± SD).

Characteristics of the Sampling Area
The upper, semi-natural area close to Miastko differs from the urbanized, downstream area in Darłowo.It is located on the stretch of the tributary that flows out from the Lake Studzieniczno, passes through the forest, enters into a fish farm, then flows downstream with meadows on both riverbanks into the urban area of Miastko.The potential source of plastic is limited by a low number of people walking across a small bridge near the crayfish collecting site.The sampling site in Darłowo is situated in the urban area and is under more anthropogenic stress.Anthropogenic pressure increases there during summer months due to tourist the interests of the coastal area.The river passing by the town enters the marine harbour.Traps were placed approximately 3 km from the river mouth and close to the weir on the upper water.

Identification of Plastics Items
The stomachs of crayfish were excised and analysed in the laboratory.The fragments of tissues containing microplastics were isolated using visual inspection on clean glass Petri dishes under an optical stereoscopic microscope NIKON SMZ800 (Tokyo, Japan) at 6.3× magnification to determine the plastic content.The microplastic particles were removed from isolated samples using tweezers and were not further cleaned.The isolated samples were studied in detail using µFTIR reflectance spectroscopy with the use of a

Characteristics of the Sampling Area
The upper, semi-natural area close to Miastko differs from the urbanized, downstream area in Darłowo.It is located on the stretch of the tributary that flows out from the Lake Studzieniczno, passes through the forest, enters into a fish farm, then flows downstream with meadows on both riverbanks into the urban area of Miastko.The potential source of plastic is limited by a low number of people walking across a small bridge near the crayfish collecting site.The sampling site in Darłowo is situated in the urban area and is under more anthropogenic stress.Anthropogenic pressure increases there during summer months due to tourist the interests of the coastal area.The river passing by the town enters the marine harbour.Traps were placed approximately 3 km from the river mouth and close to the weir on the upper water.

Identification of Plastics Items
The stomachs of crayfish were excised and analysed in the laboratory.The fragments of tissues containing microplastics were isolated using visual inspection on clean glass Petri dishes under an optical stereoscopic microscope NIKON SMZ800 (Tokyo, Japan) at 6.3× magnification to determine the plastic content.The microplastic particles were removed from isolated samples using tweezers and were not further cleaned.The isolated samples were studied in detail using µFTIR reflectance spectroscopy with the use of a Thermo Scientific™ Nicolet™ Continuµm™ Infrared Microscope (Madison, WI, USA) equipped with an MCT-A detector and a 15× objective lens.Each isolated sample was deposited on a clean glass slide covered with thin layer of silver and flattened with the use of the roller tool cleaned with alcohol after each sample to avoid contamination.The spectrum of the glass slide as a background was automatically subtracted from the spectrum of each sample.The spectra of organic clothing were not collected as a background, because of the difficulty in establishing its average composition within the same sample.Therefore, the identification of the samples was based on the recognition of the characteristic vibration of chemical bonds present in particular polymer.
FTIR spectra were recorded as changes in absorption as a function of wave number ranging from 600 cm −1 to 4000 cm −1 .The following conditions were used: measurement recording accuracy-4 cm −1 , optical velocity-0.4747cm −1 , aperture-100, minimal scan number-32, data spacing-0.482.The microscope was supplied with the camera which provided the images of the sample's structure.The spectral analysis of obtained spectra was controlled by the OMNIC version 9.8.372 software package equipped with tools allowing the identification of the size, shape, and colour of found microplastics.For samples with irregular shapes, the longest dimension was determined.The microplastics were identified by the presence of characteristic vibrations of characteristic chemical bonds as well as visual comparison with the reference spectra in accordance with the literature [25][26][27].The obtained spectra were additionally compared with the spectra of the commercially available infrared library database implemented in OMNIC: HR Nicolet Sampler Library.

Quality Assurance
To minimize the risk of plastic contamination from researchers' clothes, clean cotton lab coats were used for all laboratory works.
Sample processing was performed in clean facilities.Samples were covered with aluminium foil to prevent any contamination of samples from atmospheric microplastic particles.Equipment, such as scalpels, tweezers, and dissection boards, were inspected for plastics under a stereomicroscope prior to use and were rinsed with distilled water until all contamination was removed.There is no standardized methodology for blanks in the scientific literature, so our approach is described.The clean glass Petri dishes in the work area during sample processing and analysis were used as laboratory blanks, which were analysed to account for any contamination of plastics, and no microplastics were detected.

Statistical Analyses
The statistical analyses were performed using STATISTICA 12.0 Software (Statsoft, Krakow, Poland).The difference between crayfish that were contaminated and uncontaminated by plastic debris in relation to crayfish sex, size, and mass was analysed using regression analysis at p = 0.05.
Moreover, the relationships between the ingested plastic length, type, and the total length of the crayfish were calculated according to the following equation: where: The length-mass relationships are used to establish the physiological condition of the individuals.This condition of the signal crayfish individuals was calculated based on the length-mass relationship according to the equation below: The coefficients of variation R 2 for both above-mentioned relationships were determined at p = 0.05.Microsoft Excel 10.0 was used to calculate the relationships.
Differences between slopes (b) and intercepts (a) of length-mass regressions of specimens contaminated and uncontaminated by plastics were tested using analysis of covariance (ANCOVA) at p = 0.05.Before the analysis, both y and x data were log transformed to linearize the relationship (ln y = b × ln x + ln a) to improve the normality and homoscedasticity of the data.

Crayfish Characteristics
In total, 230 specimens of the signal crayfish in the size range from 45.7 to 133.23 mm and wet mass range from 2.87 to 69.19 g were analysed.No plastic debris was found in stomachs of crayfish caught in Miastko.In the lower river section-Darłowo-plastics were detected in the stomachs of crayfish of both sexes (Figure 3, Table S1   Males had higher plastic content in their stomach than females (67% of contaminated crayfish were identified as males).Crayfish with plastic debris from Darłowo were characterized by a total length (mean ± SD) of 91.42 ± 12.54 mm and a wet mass of 23.49 ± 9.43 g in comparison to all others collected in Darłowo (99 ± 12.51 mm and 31.29 ± 12.97 g, respectively).From the regression analysis, it was found that the presence of plastics in crayfish varies significantly with the length, mass, and sex of crayfish with correlation coefficients of R 2 = 0.15, 0.02, and 0.02.The corresponding obtained F-value is 4.95 with a significance level of p = 0.05.
Most crayfish (12) had a single plastic particle in their stomachs.Two particles in stomachs were found only in three specimens.The presence of plastics was mainly limited to crayfish in the length range from 80 to 100 mm (67% of specimens), which contained mainly PTFE and a higher number of plastic particles-up to two per individual (Figure 4).The relationship between crayfish size and plastic particles size consumed was poor and not significant (R 2 = 0.015) (Figure 4).A stronger relationship was found between microplastic fibres and crayfish sizes (R 2 = 0.1628) but was also no significant.The mass of the specimens was significantly correlated with the length of both crayfish contaminated by plastics (R 2 = 0.9704) and uncontaminated (R 2 = 0.8834), at p = 0.05.Although we expected a lower condition of specimens contaminated by plastics, the relationships between the mass and length of specimens that were contaminated and uncontaminated by plastics showed that the calculated slope "b" was high and similar.The physiological condition of the crayfish collected in Darłowo was almost the same for specimens with (b = 3.1465) (Figure 5) and without (b = 3.1501) plastic debris in the stomachs (Figure 5).Statistical differences between slopes (b) and intercepts (a) of specimens contaminated and uncontaminated by plastics were insignificant (ANCOVA, p > 0.05).

Plastic Debris Characteristics
All plastic debris found in the crayfish stomachs (Figure S1 in Supplementar rials) were different types, sizes, shapes, and colours (Table 1).Detailed polymer indicated that plastics consisted of round particles with mainly irregular fra (opaque), fibres (transparent, blue, and red) or thin pieces (blue or colourless).The sions of microplastics ranged from 70 to 450 µm.The presence of PTFE (polytetr ethylene), cellophane, PP (polypropylene), PE (polyethylene), PMMA (polymethy acrylate), and nylon was identified by FTIR spectroscopy.

Plastic Debris Characteristics
All plastic debris found in the crayfish stomachs (Figure S1 in Supplementary Materials) were different types, sizes, shapes, and colours (Table 1).Detailed polymer analysis indicated that plastics consisted of round particles with mainly irregular fragments (opaque), fibres (transparent, blue, and red) or thin pieces (blue or colourless).The dimensions of microplastics ranged from 70 to 450 µm.The presence of PTFE (polytetrafluoroethylene), cellophane, PP (polypropylene), PE (polyethylene), PMMA (polymethyl methacrylate), and nylon was identified by FTIR spectroscopy.
Polytetrafluoroethylene (PTFE) polymer was isolated as black rugged clods from six crayfish.It was the most frequent polymer in the analysed stomachs.The FTIR spectrum shows the presence of two very intense bands of stretching vibrations of the CF 2 group at about 1220 and 1157 cm −1 .
Cellophane was isolated from five specimens.It was characterized with the following bands: a broad peak at 3324 cm −1 representing OH stretching vibrations, 2913 and 2850 cm −1 representing CH stretching, a peak at about 1363 cm −1 attributed to OH bending, 1310 cm −1 representing CH 2 wagging, and a band at about 1153 cm −1 characteristic of C-O antisymmetric bridge stretching and C-O-C pyranose ring skeletal vibrations.Moreover, in the spectrum of cellophane, a strong band at about 1013 cm −1 is present, which can be assigned as C-O-C stretching, and a small band at 889 cm −1 that corresponds to the glycosidic C-H deformation with ring vibration contribution and O-H bending.Polypropylene (PP) was isolated from three crayfish as fragments of blue foil or thin red filaments and the most characteristic vibrations for PP are 2952, 2915, and 2837 cm −1 , typical of symmetric and asymmetric C-H stretching, as well as 1455 and 1377 cm −1 , which represent CH 2 and CH 3 bending vibrations, respectively.
Polyethylene (PE) was found in two crayfish and had the forms of colourless, blue, and red film pieces.The characteristic absorbance bands for PE are located at 2901 and 2843 cm −1 , typical of asymmetric and symmetric vibrations, as well as 1460 and 713 cm −1 , typical of bending and rocking CH 2 deformations.
Polymethyl methacrylate (PMMA) was found in a single crayfish and identified with vibrations in the 2960-2840 cm −1 range, typical of a stretching region of CH, CH 2 , and CH 3 .Pure PMMA can be described by the 1736 cm −1 band assigned to free C=O stretching, although in the spectrum of the analysed sample, this band is shifted to a lower wavenumber (1707 cm −1 ).C-O stretching frequency is one of the intense bands that appear in the fingerprint region, usually within 1000-1400 cm −1 .The bands present in the 1260-1000 region can be assigned to C-O stretching modes, whereas the vibrations of C=O in the plane and out of plane bending are assigned to a medium-strong band in the IR spectrum at about 720 cm −1 .
Nylon as a blue small filament was identified in a single crayfish with the presence of stretching vibrations of NH groups at 3290 cm −1 and bending NH vibrations at 1535 cm −1 , as well as the stretching carbonyl C=O group at 1638 cm −1 and the CH vibrations at 2914 and 2847 cm −1 .

Discussion
Recently, the problem of the land-based transport of plastics to river systems has been described [28].Microplastics in macroinvertebrates can indicate plastic pollution within a catchment [29], and our study has, for the first time, shown the occurrence of plastic debris in the stomachs of signal crayfish, although previous observations reported the plastics in different aquatic organisms, e.g., described in refs.[11,30] and summarized by de Sá et al. in [31].
In our study, plastic debris was confirmed in 7.3% of analysed specimens in the lower, urbanized part of the Wieprza River, which has been under severe anthropogenic pressure for a long period.Such an impact is not only observed in Darłowo but also in many areas under anthropogenic pressure worldwide [32].Our study found a lack of plastic debris in the upper, semi-natural section of the Wieprza River tributary which flows from the Lake Studzieniczno to Miastko (Figure 2).However, 2 out of 25 specimens collected there should have contained plastics, so the difference in sampling size of 25 vs. 205 is not crucial for our conclusion.The smaller number of crayfish collected upstream in comparison to the downstream river section was related to lower numbers of signal crayfish in this part of the Wieprza River system.This was confirmed in the previous study [24].However, microplastic accumulation in the sediment is modified by fluvial processes, which result in lower levels in the upper river course, as was observed in the Inde River [33].
Plastic debris seems to be rather rare in crayfish stomachs and was identified in only 7.3% among specimens caught in Darłowo.Similarly low plastic content (9%) was observed in Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 in the Vistula Lagoon (Poland) [34].These results differ from another study where plastics were found in approximately 50% of analysed macroinvertebrate samples from UK rivers [30], which might be explained by larger historic plastic production in western European countries compared to Poland (central states) [35,36].The occurrence of plastics in crayfish stomachs, based on our results, does not appear to be a good indicator for measuring the contamination of the river environment.Present results indicating relatively low plastic debris content in crayfish stomachs may be explained by animal behaviour and, to some extent, by the local environmental conditions.Specimens of the signal crayfish are characterized by their activity in the river manifested through burrowing behaviour and movement [37][38][39].They may spend a long time in burrows or may migrate in both upstream and downstream directions, crossing sediments contaminated by plastics.In the case of crayfish that reached the trapping site in Darłowo, most move downstream.Therefore, they might spend more time in the stretches of the river with a relatively lower level of contamination by plastic compared with the urbanized river mouth.The ingestion of plastics by the signal crayfish (determined as the occurrence of plastics in stomachs) differs from the sessile species such as mussels, which experience permanent exposure to plastic pollution and accumulate significant quantities of microplastics, depending on the pollution loading where they live [40].
Visual classification with the use of a microscope allowed us to identify "plastic debris" in 52 individuals (22.6% of all analysed crayfish).But, to avoid misidentification and the underestimation of microplastics it was necessary to use the more accurate method, such as Fourier transform infrared spectroscopy (FTIR).FTIR analysis confirmed plastics in 15 individuals (28.8% of visually identified presence of plastics).This step was critical since often about 70% of particles that visually resemble microplastics are not confirmed as plastics by FTIR spectroscopy [41].Additionally, based on FTIR analysis, the determination of the type of polymer was possible by the identification of six different types of polymers in the stomachs of P. leniusculus from the Wieprza River system, namely PTFE (polytetrafluoroethylene), cellophane, PP (polypropylene), PE (polyethylene), PMMA (polymethyl methacrylate), and nylon.According to Duis and Coors [42], the most frequent polymers in aquatic environments are PE, PP, and PS.However, in our study, PTFE was the most common.This is a high-performance fluoropolymer, commonly used in the kitchen (pans, baking trays, etc.), and has wide applications in chemical, electronic, construction and car industries.As a highly inert and nontoxic polymer, it also finds use in medical applications for cardiovascular grafts, heart patches, etc. [43].It is also the most chemically resistant polymer with very high stability, which is not subject to degradation in the environment [44].
One of the most important factors affecting microplastics distribution in aquatic environments is the density of the materials.The relatively high frequency of PTFE in the crayfish stomachs may be explained to some extent by different accumulation among various types of plastics in the riverine sediment [45].Due to the hydrodynamic properties of the plastic particles, such as density, particle size, shape, surface roughness, and the hydrodynamic transport conditions in the river, PTFE is the most frequent type of plastic deposited between 0 and 30 cm depth of sediment, that is, the habitat zone of the signal crayfish [46].Other polymers, such as PE and PP, transfer to deeper layers of fluvial sediments [45].
Fibres were the predominant type of microplastics in crayfish stomachs, and also the most common form of plastics described in previous studies [47][48][49].The size of plastic debris ingested depends on the animal size [50].However, during the digestion process, the fragmentation of plastics occurs in crustaceans such as Norway lobster Nephrops norvegicus (Linnaeus, 1758) [51] and results in the occurrence of tinier plastic fragments in the environment.Such particles might be available for smaller organisms at lower trophic levels.The size of plastic particles is a crucial factor in determining their uptake, digestion, and toxicity.Smaller particles (<10 µm) may have a higher negative impact with increased potential to interact at the molecular level than larger ones (summarized in [52]).
The ingestion of plastics might be harmful by blocking the crustaceans' stomachs, causing a reduction in their feeding [53].The blockage and injury of the digestive tract and reduction in feeding caused by ingested plastic and the absorption of polychlorinated biphenyls in fish has been recorded over the past decades and considered by ref. [54] after refs.[55,56].The chemical decomposition of some polymers may also lead to the formation of toxic compounds, e.g., the oxidative degradation of PTFE in atmospheric conditions results in the evolution of gaseous carbonyl fluoride COF 2 , which is highly toxic and hydrolyses further to hydrofluoric acid, HF, and carbon dioxide, CO 2 [57].The condition of collected crayfish in this study with plastic debris in stomachs was as good as specimens without plastics.This is not surprising, because microplastics cause no effects on the survival and growth of many invertebrates, but they still may affect the functioning of only sensitive aquatic species [58].Moreover, recent studies [59] showed that the negative impacts of microplastics may not be readily visible at the ecosystem scale.
The size (70-450 µm) and abundance of plastic particles (1-2 per individual) were very low considering the signal crayfish stomach size (including cardiac and pyloric parts of the stomach) is about 20% of total body length [60].Moreover, the presence of plastics, limited mainly to crayfish specimens in the length range between 80 and 100 mm, with predomination in males, may be connected with food preferences and mechanisms related to the crayfish size, sex (considering diet specialization), and moulting.Such crayfish feed mainly on small food [61], so microplastic particles are more frequently swallowed by this group of specimens.The lower percentage of females with plastics in their stomachs than males may result from sexual dimorphism and different behaviour.Females are characterized by smaller carapace size and claws, different mandible shape, and lower feeding [61,62], which may result in a lower probability of plastic consumption.The smallest crayfish shed their carapaces more frequently than larger specimens.During the process of moulting, they may potentially eliminate plastic debris.Crustaceans lose their integument (the foregut and hindgut) during ecdysis, which is moulted along with the exoskeleton [53].This process explains the lack of plastics in the smallest crayfish.Low plastic content in crayfish stomachs is also connected with higher water temperature during summer months when the process of moulting is frequent [63].Moreover, the largest crayfish do not have plastics in their stomachs, because of different feeding preferences-they prefer larger food size [61].Small particles (even detritus) may be also consumed by large crayfish [46]; similarly, plastic might be accidentally eaten by crayfish during normal feeding.It is possible that crayfish mistake microplastics for prey.
The only eaten parts of the crayfish are the abdomen and chelae that consist of muscle tissue, and the remains (cephalothorax) with microplastics in the stomachs and intestines are thrown away.The plastic debris may potentially transfer toxic substances [64], but without data on their concentration in the muscle tissue of the crayfish, their impact on human health remains under speculation.However, potential contamination of plastic particles in muscle tissue by monomers which occur in the circulatory system of these animals should be studied in the future.

Conclusions
Plastic debris was found in the stomach of the invasive signal crayfish from the Wieprza River, the Baltic tributary.Plastic contamination depended on anthropogenic impact on the river and distance from the river mouth.Plastic debris had a size range between 70 and 450 µm.Six types of polymers, namely PTFE (polytetrafluoroethylene), cellophane, PP (polypropylene), PE (polyethylene), PMMA (polymethyl methacrylate), and nylon, were identified using the Fourier transform infrared spectroscopy (FTIR) technique.PTFE was the most common polymer, but according to the literature, it is rarely detected in the environment and organisms.Transparent, blue, and red fibres dominated over other types of plastic debris.Microplastics did not affect crayfish growth rate in our study.The lower percentage of females with plastics may result from sexual dimorphism and different behaviour.Future study should concentrate on signal crayfish contamination by other chemicals, e.g., persistent organic pollutants (POPs), and the potential risk to humans [65].

Data Availability Statement:
The data presented in this study are available within the article.

Figure 1 .
Figure 1.Graphic presentation of the study on the signal crayfish Pacifastacus leniusculus in the Wieprza River system (Poland).Figure 1. Graphic presentation of the study on the signal crayfish Pacifastacus leniusculus in the Wieprza River system (Poland).

Figure 1 .
Figure 1.Graphic presentation of the study on the signal crayfish Pacifastacus leniusculus in the Wieprza River system (Poland).Figure 1. Graphic presentation of the study on the signal crayfish Pacifastacus leniusculus in the Wieprza River system (Poland).

Figure 2 .
Figure 2. Sampling sites of the signal crayfish Pacifastacus leniusculus individuals collected in the Wieprza River system (Poland).

Figure 2 .
Figure 2. Sampling sites of the signal crayfish Pacifastacus leniusculus individuals collected in the Wieprza River system (Poland).
M w = a × L T b Water 2024, 16, 903 5 of 13 where: M W -wet mass in mg, L T -crayfish total length in mm, a-intercept, b-slope.

Figure 3 .Figure 3 .
Figure 3. Plastic debris in the stomachs of the signal crayfish Pacifastacus leniusculus collected in the Wieprza River system.

Figure 3 .Figure 4 .
Figure 3. Plastic debris in the stomachs of the signal crayfish Pacifastacus leniusculus collected in Wieprza River system.

Figure 4 .
Figure 4. Relationships between the size of ingested microplastics confirmed by FTIR and the total length of the signal crayfish Pacifastacus leniusculus collected in the Wieprza River in Darłowo: (a) information on the type of plastics, (b) information on the plastic length.

Figure 4 .
Figure 4. Relationships between the size of ingested microplastics confirmed by FTIR and length of the signal crayfish Pacifastacus leniusculus collected in the Wieprza River in Dar information on the type of plastics, (b) information on the plastic length.

Figure 5 .
Figure 5. Relationships between total length and wet mass of the signal crayfish Pacifastacu culus (a) with plastics and (b) without plastics in stomachs collected in the Wieprza Darłowo.

Figure 5 .
Figure 5. Relationships between total length and wet mass of the signal crayfish Pacifastacus leniusculus (a) with plastics and (b) without plastics in stomachs collected in the Wieprza River in Darłowo.
in Supplementary Materials).

Table 1 .
The sizes, shapes, and colours of polymers found in the stomachs of the signal Pacifastacus leniusculus collected in Darłowo.
specimens without plastics in stomachs

Table 1 .
The sizes, shapes, and colours of polymers found in the stomachs of the signal crayfish Pacifastacus leniusculus collected in Darłowo.