pH-Responsive Eco-Friendly Chitosan–Chlorella Hydrogel Beads for Water Retention and Controlled Release of Humic Acid

: For improving the mechanical strength of controlled release fertilizer (CRF) hydrogels, a novel material of Chlorella was employed as a bio-based ﬁller to prepare chitosan–chlorella hydrogel beads with physical crosslink method. Here, the synthesis mechanism was investigated, and the chitosan–chlorella hydrogel beads exhibited enhanced mechanical stability under centrifugation and sonication than pure chitosan hydrogel beads. Chlorella brought more abundant functional groups to original chitosan hydrogel, hence, chitosan–chlorella hydrogel beads represented greater sensitivity and controllable response to external factors including pH, salt solution, temperature. In distilled water, the hydrogel beads with 40 wt% Chlorella reached the largest water absorption ratio of 42.92 g/g. Moreover, the mechanism and kinetics process of swelling behavior of the chitosan– chlorella hydrogel beads were evaluated, and the loading and releasing of humic acid by the hydrogel beads as a carrier material were pH-dependent and adjustable, which exhibit the potential of chitosan– chlorella hydrogel beads in the ﬁeld of controlled release carrier biomaterials.


Introduction
Fertilizer is an indispensable part of modern agriculture. The proper application of fertilizers can maintain soil fertility and improve crop yields. However, during the volatilization and leaching process, fertilizer losses have greatly reduced the utilization efficiency, increased agricultural costs and threatened environmental pollution, especially in developing countries [1]. Recently, controlled release fertilizers have received considerable attention worldwide with the potential to achieve a partial synchronization of nutrient release and physiological need of plants which can effectively prevent fertilizer losses [2]. Polymer, using as the initial material of hydrogel, has been proven to be an effective prolongedrelease carrier for the advantage of absorbing a high amount of water and release it over a long period of time in combination with a fertilizer [3][4][5]. The combination of hydrogel and fertilizer, like a "mini reservoir", reduces both the environmental impact of fertilizer and evaporation losses. However, a large amount of polymers such as poly(acrylic acid), poly(acrylamide), and copolymer are difficult to biodegrade [3]. Consequently, seeking biodegradable, abundant, and low-cost natural polymers for controlled release fertilizers have become a priority for engineers [6].
Chitosan, the product of deacetylation of the natural polysaccharide chitin, is a commonly used biomaterial composed of β-(1-4)-2-acetamido-D-glucose and β-(1-4)-2-amino-D-glucose units, with a pK a value varying from 6.3 to 6.5 [7,8]. Chitin, mainly derived from crustaceans such as shrimp and crab, is one of the most plentiful biopolymers on Earth [9], and hence crab and shrimp shell waste provides a sufficient source for the industrial production of chitosan. There are three types of reactive functional groups in chitosan, an crops for valuable arable land. To our knowledge, there are few studies using Chlorella as a bio-based filler to enhance the properties of natural polymers.
The awareness about the environment and sustainability has led to the demand for alternative petroleum-based polymer products, such as natural polymers reinforced with bio-based fillers. Inspired by these, Chlorella has been selected as a novel material to employ as a bio-based filler to reinforce polymer performance. In this paper, chitosan-chlorella hydrogel beads have been manufactured through the physical crosslinking method. Considering that the cellular structure and biochemical properties of Chlorella can strengthen the mechanical strength and water absorption properties of chitosan hydrogels, we investigate the relevant properties of the prepared chitosan-chlorella hydrogels. Similarly, the surface structure of the chitosan-chlorella hydrogel beads was investigated with scanning electron microscopy (SEM), and their structure was characterized with Fourier transform infrared spectroscopy (FTIR). Moreover, we discuss the swelling mechanism and kinetics to study the swelling process in water. Finally, in order to evaluate the feasibility of hydrogel beads for controlled release fertilizers, their pH and temperature stimuli-responsive release behavior for the slow release of humic acid was investigated.

Synthesis of Chitosan-Chlorella Hydrogel Beads
A 1 g amount of chitosan was added to 50 mL 2% (v/v) acetic acid solution, stirring constantly to dissolve the chitosan. Then, a certain amount of Chlorella powder was added to the chitosan solution and stirred continuously for 1 h. The above blended solution was dropped into a 2 M NaOH solution using a syringe needle with a diameter of 0.6 mm. This results in the formation of alkaline hydrogel beads. The formed chitosan-chlorella beads were left in NaOH solution for another 3 h for gelation. The beads were separated from the NaOH solution with a metal sieve and washed repeatedly until the solution pH became neutral. Then, the resulting sample was dried at room temperature to obtain the chitosan-chlorella hydrogel beads.

Materials Characterization
The dried chitosan and chitosan-chlorella hydrogel beads were ground into powder. Then, 1 mg of the powder was blended with 100 mg of KBr in an agate mortar and pressed into flakes after grinding evenly. Afterwards, FTIR infrared spectra were measured at 4000-450 cm -1 wavenumber in a Nicolet FTIR spectrometer. Surface morphology, shape, and size of the chitosan-chlorella hydrogel beads were investigated by scanning electron microscope.

Degree of Swelling Measurements
A known weight of dry hydrogel sample was submerged in 250 mL swelling media (distilled water, various salt solutions, and buffer solutions with desired pH) and remained at room temperature for 4 h until the swelling equilibrium was reached. The sample was removed and absorbed the excess water with filter paper and weighed. The degree of swelling was obtained by the following formula: where W e and W i are the weights of the hydrogel beads' swelling equilibrium and initial weights, respectively. In other words, the degree of swelling is calculated according to the grams of water absorbed per gram of sample.

Determination of Mechanical Stability
The mechanical stability of chitosan-chlorella hydrogel beads was judged by the response of the samples to ultrasonic vibration and centrifugation at high speed. The dried samples were left in distilled water for 4 h to reach swelling equilibrium. Then, a certain mass of swelling hydrogel beads was centrifuged at 4000 rpm or ultrasonified at 40 kHz, respectively. The mass of samples retained was weighed at distinct time intervals (t; min). The weight retention rate was obtained by the following formula: where W e and W a are the wet weights of hydrogel beads before and after centrifugation or ultrasonification, respectively.

Loading and Slow-Release Efficiency for Humic Acid
To study the potentiality of chitosan-chlorella hydrogel beads as controllable loading and release carriers, humic acid was selected as a model fertilizer. Humic acid was loaded into the hydrogel beads with 40 wt% Chlorella content, so as to explore its feasibility in the controllable loading and release system. The 0.5 g dried hydrogel beads were placed in 50 mL 30 ug/mL humic acid with pH from 4 to 12 and then shaken in a constant temperature shaker at 120 rpm for 30 min at 30 • C. The humic-acid-loaded hydrogel beads were oven dried at 30 • C after filtration separation. The concentration of humic acid solution in the supernatant was calculated by absorbance at 259.6 nm wavelength with a 752 UV-vis spectrophotometer [25]. The loading efficiency of humic acid was calculated by the following equation: where C 0 and C 1 are the concentrations at the initial and end of the loading of humic acid, respectively. The humic-acid-loaded samples were immersed in distilled water with various pH and shaken in a constant temperature shaker at 120 rpm for 3 h. The concentration of humic acid in the mixed solution was determined at intervals, and the release efficiency of humic acid was calculated by the following equation: where C 2 is the concentration of humic acid after release.

Synthesis and Characterization of Chitosan-Chlorella Hydrogel Beads
The preparation mechanism of the chitosan-chlorella hydrogel beads is shown in Figure 1. Chitosan, with a pK a value of about 6.3, can be dissolved in dilute acid, but not in water or alkaline medium because the amino group (-NH 2 ) in the structural unit can be reacted with acid readily and converted into soluble protonated amino group (-NH 3 + ) [26].
Therefore, an acidic solution was often used as the solvent for dissolving chitosan. In this study, a 2% acetic acid solution was used to dissolve chitosan. After a long time stirring, chitosan powder was completely dissolved in acetic acid solution, during which the hydroxyl, amino, and N-acetylamino groups distributed on the chitosan macromolecule chain interact to form various intra-and intermolecular hydrogen bonds. As a result, the molecular chains of chitosan were intertwined under the action of hydrogen bonding and van der Waals forces, thus forming a three-dimensional network structure [27]. Meanwhile, the -NH 2 group of glucosamine monomer on chitosan molecular chains were protonated into positively charged -NH 3 + groups in acidic environment, and electrostatic repulsion between -NH 3 + groups expanded the three-dimensional network. Chlorella powder was subsequently added into chitosan solution, the expansion caused by electrostatic repulsion made it easier for Chlorella cells to disperse and embed into the chitosan network uniformly. Then, the chitosan-chlorella mixture solution was dripped into sodium hydroxide solution through a needle. At alkaline conditions, -NH 3 + groups were deprotonated, and the solubility of chitosan was reduced and precipitated out of the solution, resulting in the formation of hydrogel beads. During the synthesis process, the changing process from high viscosity droplets to hydrogel beads can be clearly observed, and these changes in its shape and morphology confirmed the formation of hydrogel. Therefore, it can be concluded that the Chlorella cells were successfully encapsulated into the chitosan cross-linked network and the chitosan-chlorella hydrogel beads were synthesized.  The FTIR spectra of chlorella, chitosan beads, and chitosan-chlorella hydrogel beads are shown in Figure 2. For chitosan, the peaks at 1659 and 1326 cm −1 are attributed to the -NH2 deformation vibration and C-N stretching vibration, respectively [28]. The characteristic absorption band at 3362, 1598, and 1095 cm −1 are attributed to O-H hydroxyl group, N-H angular deformation, and C-O-C in glycosidic linkage, respectively [29]. For Chlorella, the broad peak at 3301 cm −1 is due to the stretching vibration of intermolecular hydroxyl, and the peak at 1079 cm −1 is attributed to the C-O stretching vibration, which indicate the presence of abundant hydroxyl groups on Chlorella [30]. Furthermore, the characteristic absorption band at 1658, 1546, 1401, and 1241 cm −1 are from C=O stretching vibration (amide I), N-H bending vibration (amide II), and C-N absorption band (amide III) in the amide, respectively [31]. In the FTIR spectra of chitosan-chlorella hydrogel beads shown in curves c and d, hydrogel beads with 20 wt% and 40 wt% Chlorella retained most of the characteristic peaks of chitosan and Chlorella. However, the absorption peaks of chitosan at 3362, 1095 cm −1 and Chlorella at 3301, 1079 cm −1 shifted in different degrees. Meanwhile, the absorption peaks of chitosan at 1659 and 1326 cm −1 were also shifted. The shift in the characteristic peaks indicated that in the three-dimensional network of hydrogel beads, chitosan and Chlorella cells were connected and entangled together mainly through intermolecular hydrogen bonds between amino and hydroxyl groups, which result in a stable three-dimensional network.   Figure 3 provides the particle size image and SEM micrographs, which deliver th information on the size, shape, and surface topography of the hydrogel beads. It can b seen from Figure 3a that the diameter of swollen chitosan-chlorella hydrogel beads had an average size of 3 mm, while the average diameter of dried beads is about 0.9 mm. Th increase of particle size indicated that the beads have a certain swelling ability. It can b seen that Chlorella cells are clustered with a diameter of 3-4 microns in Figure 3b. Th chitosan-chlorella hydrogel beads shown in Figure 3d had a rougher surface and exhib ited more severe shrinkage resulting from drying compared to the chitosan hydroge beads with more perfect spherical shape in Figure 3c. However, the shrunken beads could still recover to full spherical shape after swelling, which was attributed to the three-di mensional network of hydrogel and stability of Chlorella cells. Figure 3e,f were the surfac microtopography images of chitosan hydrogel beads and chitosan-chlorella hydroge beads, respectively. Chitosan-chlorella hydrogel beads had more pores on the surface  Figure 3 provides the particle size image and SEM micrographs, which deliver the information on the size, shape, and surface topography of the hydrogel beads. It can be seen from Figure 3a that the diameter of swollen chitosan-chlorella hydrogel beads had an average size of 3 mm, while the average diameter of dried beads is about 0.9 mm. The increase of particle size indicated that the beads have a certain swelling ability. It can be seen that Chlorella cells are clustered with a diameter of 3-4 microns in Figure 3b. The chitosan-chlorella hydrogel beads shown in Figure 3d had a rougher surface and exhibited more severe shrinkage resulting from drying compared to the chitosan hydrogel beads with more perfect spherical shape in Figure 3c. However, the shrunken beads could still recover to full spherical shape after swelling, which was attributed to the threedimensional network of hydrogel and stability of Chlorella cells. Figure 3e,f were the surface microtopography images of chitosan hydrogel beads and chitosan-chlorella hydrogel beads, respectively. Chitosan-chlorella hydrogel beads had more pores on the surface, with the average diameter of these pores being about 0.2 µm. This porous structure brought about excellent adsorption properties to the hydrogel beads. In conclusion, landfilling of Chlorella cells enhanced the performance of hydrogel beads and exerted the advantages of composite materials.
with the average diameter of these pores being about 0.2 μm. This porous struc brought about excellent adsorption properties to the hydrogel beads. In conclusion, l filling of Chlorella cells enhanced the performance of hydrogel beads and exerted the vantages of composite materials.

Mechanical Stability of Chitosan-Chlorella Hydrogel Beads
The mechanical stability of the chitosan-chlorella hydrogel beads was measure the wet weight retention under ultrasound and centrifugation. Figure 4a shows the w retention of samples with different Chlorella content after a certain time of centrifuga The van der Waals forces and hydrogen bonding, which are widely present between drogels and water molecules, determined the water retention properties of hydrogels In the first 20 min, the water retention rate decreased sharply, and then gradually slo down. The reason for this phenomenon was that, the hydrogel beads contained m weakly bound water that was easier lost, and with the increase of centrifugal or ultras time, the proportion of bound water that is difficult to separate became higher, resu in a slower dehydration rate. After centrifugation for 60 min, the weight retention of tosan hydrogel beads containing Chlorella was higher than that without Chlorella, and higher the content of Chlorella in the beads, the higher the weight retention of bead other words, the presence of Chlorella enhanced the mechanical stability of hydr beads. The cross-linking of Chlorella with the chitosan network has strong hydrogen b ing interactions, the hydrogen bonds served as a crack bridge to retain an intact struc and stabilize the deformations of centrifugation. For example, Cong et al. have conclu that the intertwined hydrogen-bonding network of hydrogel could effectively relax locally applied stress and dissipate the crack energy to improve the mechanical stre of hydrogel beads [33]. On the other hand, the natural toughness of the cell wall of C rella is resistant to the compressive forces brought about by centrifugation, preventin hydrogel beads from being severely compressed.
Similar results were obtained from the ultrasonic experiments of chitosan-chlo hydrogel beads as shown in Figure 4b. After ultrasonication for 60 min, water reten

Mechanical Stability of Chitosan-Chlorella Hydrogel Beads
The mechanical stability of the chitosan-chlorella hydrogel beads was measured by the wet weight retention under ultrasound and centrifugation. Figure 4a shows the water retention of samples with different Chlorella content after a certain time of centrifugation. The van der Waals forces and hydrogen bonding, which are widely present between hydrogels and water molecules, determined the water retention properties of hydrogels [32]. In the first 20 min, the water retention rate decreased sharply, and then gradually slowed down. The reason for this phenomenon was that, the hydrogel beads contained more weakly bound water that was easier lost, and with the increase of centrifugal or ultrasonic time, the proportion of bound water that is difficult to separate became higher, resulting in a slower dehydration rate. After centrifugation for 60 min, the weight retention of chitosan hydrogel beads containing Chlorella was higher than that without Chlorella, and the higher the content of Chlorella in the beads, the higher the weight retention of beads. In other words, the presence of Chlorella enhanced the mechanical stability of hydrogel beads. The cross-linking of Chlorella with the chitosan network has strong hydrogen bonding interactions, the hydrogen bonds served as a crack bridge to retain an intact structure and stabilize the deformations of centrifugation. For example, Cong et al. have concluded that the intertwined hydrogen-bonding network of hydrogel could effectively relax the locally applied stress and dissipate the crack energy to improve the mechanical strength of hydrogel beads [33]. On the other hand, the natural toughness of the cell wall of Chlorella is resistant to the compressive forces brought about by centrifugation, preventing the hydrogel beads from being severely compressed. of the chitosan hydrogel network. Therefore, the hydrogen bond cross-linked network formed by chitosan with Chlorella could better withstand ultrasonic fragmentation and keep the stability of beads. In summary, filling Chlorella into the hydrogel network with the effect of intermolecular forces can effectively reduce the water loss and improve the mechanical stability.

Equilibrium Swelling Ratio at Various pH Solutions and Pulsatile Behavior
It is well known that the swelling behavior of hydrogels is significantly related to the pH of the swelling medium [34,35]. Therefore, the variety of the swelling ratio of chitosanchlorella hydrogel beads based on pH change was investigated, and the swelling medium was buffer solutions with pH ranging from 2 to 10 at room temperature. In this study, the influence of ionic strength during the swelling process was avoided by using HAc-NaAc buffer solution, and the pH was controlled only by the dropwise addition of HCl or NaOH solution. Figure 5a demonstrates the swelling behavior of hydrogel beads with different contents of Chlorella in the buffer solution of pH from 2 to 10. The chitosan-chlorella hydrogel beads reached maximum swelling at pH 6~8, and the order of the swelling ratios for samples with different Chlorella content was: 40 wt% > 30 wt% > 50 wt% > 20 wt% > 0 wt%. When the Chlorella content was lower than 40 wt%, the swelling degree increased with the increase in the Chlorella content. This is because the Chlorella cell may serve as a site for the storage of water in the cross-linked network, and the water molecules enter the intracellular of Chlorella after entering the cross-linked network by osmosis. This suggests that the presence of Chlorella enhanced the swelling capacity of the hydrogel beads. When the pH is lower than 6, the swelling degree increases with the increase in the pH value. However, when the pH exceeds 8, the swelling degree started to go down. The reason for this result was that when the pH value of the external solution was lower than the pKa Similar results were obtained from the ultrasonic experiments of chitosan-chlorella hydrogel beads as shown in Figure 4b. After ultrasonication for 60 min, water retention of the samples with higher Chlorella content was significantly higher than those with lower Chlorella contents. This indicated that the hydrogel beads formed by Chlorella with chitosan were more stable than the pure chitosan hydrogel beads in mechanical properties. The hydrophilic functional groups on the surface of Chlorella cells were linked together by strong hydrogen bonding interactions with -OH and -NH 2 groups on chitosan; thereby, the crosslinking of Chlorella cells and chitosan network increased the density of the crosslinked network and improved the physical strength of chitosan-chlorella hydrogel beads. In other words, the presence of abundant hydrogen bonds reinforced the skeletal structure of the chitosan hydrogel network. Therefore, the hydrogen bond cross-linked network formed by chitosan with Chlorella could better withstand ultrasonic fragmentation and keep the stability of beads. In summary, filling Chlorella into the hydrogel network with the effect of intermolecular forces can effectively reduce the water loss and improve the mechanical stability.

Equilibrium Swelling Ratio at Various pH Solutions and Pulsatile Behavior
It is well known that the swelling behavior of hydrogels is significantly related to the pH of the swelling medium [34,35]. Therefore, the variety of the swelling ratio of chitosan-chlorella hydrogel beads based on pH change was investigated, and the swelling medium was buffer solutions with pH ranging from 2 to 10 at room temperature. In this study, the influence of ionic strength during the swelling process was avoided by using HAc-NaAc buffer solution, and the pH was controlled only by the dropwise addition of HCl or NaOH solution. Figure 5a demonstrates the swelling behavior of hydrogel beads with different contents of Chlorella in the buffer solution of pH from 2 to 10. The chitosan-chlorella hydrogel beads reached maximum swelling at pH 6~8, and the order of the swelling ratios for samples with different Chlorella content was: 40 wt% > 30 wt% > 50 wt% > 20 wt% > 0 wt%. When the Chlorella content was lower than 40 wt%, the swelling degree increased with the increase in the Chlorella content. This is because the Chlorella cell may serve as a site for the storage of water in the cross-linked network, and the water molecules enter the intracellular of Chlorella after entering the cross-linked network by osmosis. This suggests that the presence of Chlorella enhanced the swelling capacity of the hydrogel beads. When the pH is lower than 6, the swelling degree increases with the increase in the pH value. However, when the pH exceeds 8, the swelling degree started to go down. The reason for this result was that when the pH value of the external solution was lower than the pK a (approximately 6.3) of chitosan, chitosan's amino groups were protonated, thus electrostatic attraction between -NH 3 + dilates the cross-linked network of the chitosan-chlorella hydrogel, which leads to an enhancement in swelling. However, the hydrogen bond formed by -OH on chitosan hindered the expansion of the network. In addition, chitosan can be dissolved in acidic solution. The corrosion of the chitosan-chlorella hydrogel beads by the external solution destroys the crosslinking network structure and leads to the decrease of swelling degree. Conversely, at high pH (above about 8), the -NH 3 + on chitosan was deprotonated to amino group, the influence of electrostatic attraction on crosslinking network is weakened. Besides, the hydrogen bonding induced by -OH and -NH 2 and the "screening effect" of excess Na + in external solution are also the reasons for the decrease of swelling degree [36].
crease of swelling degree. Conversely, at high pH (above about 8), the -NH3 + on chitosan was deprotonated to amino group, the influence of electrostatic attraction on crosslinking network is weakened. Besides, the hydrogen bonding induced by -OH and -NH2 and the "screening effect" of excess Na + in external solution are also the reasons for the decrease of swelling degree [36].
In order to study the swelling reversibility of beads, the chitosan-chlorella hydrogel beads with optimal water absorption were selected to be alternately immersed in solutions at two pH values of 6 and 10 to study pH-dependent swelling reversibility. It can be seen from the Figure 5b that the swelling of the chitosan-chlorella hydrogel beads is pH-dependence and reversible with a relatively quick speed. When the beads were in a buffer solution with pH 6, they swelled because of electrostatic repulsion due to amino protonation and shrinks at pH 10 due to deprotonation leads to the disappearance of electrostatic repulsion. The results showed the swelling-deswelling behavior of chitosan-chlorella hydrogel beads exhibited good pH-dependent reversibility and could be repeated many times.

Effect of Various Salt Solutions on Water Absorbency
The swelling degree of chitosan-chlorella hydrogel beads influenced by different cations can be summarized from Figure 6a. The water absorption of the beads in salt solution was significantly lower than that in distilled water. In the swelling of ionic hydrogels, which is a common phenomenon, it is usually due to anion-anion electrostatic repulsion caused by the charge screening effect of cations and osmotic pressure difference between the inside and outside of the hydrogel network [37]. As can be seen from Figure 6a, the swelling ratio of hydrogel beads in different salt solutions were ranked from highest to lowest as KCl > NaCl > MgCl2 > CaCl2 > AlCl3. This may be due to the complexation of polyvalent metal cations and hydroxyl groups on chitosan and the influence of ionic strength. In order to study the swelling reversibility of beads, the chitosan-chlorella hydrogel beads with optimal water absorption were selected to be alternately immersed in solutions at two pH values of 6 and 10 to study pH-dependent swelling reversibility. It can be seen from the Figure 5b that the swelling of the chitosan-chlorella hydrogel beads is pH-dependence and reversible with a relatively quick speed. When the beads were in a buffer solution with pH 6, they swelled because of electrostatic repulsion due to amino protonation and shrinks at pH 10 due to deprotonation leads to the disappearance of electrostatic repulsion. The results showed the swelling-deswelling behavior of chitosanchlorella hydrogel beads exhibited good pH-dependent reversibility and could be repeated many times.

Effect of Various Salt Solutions on Water Absorbency
The swelling degree of chitosan-chlorella hydrogel beads influenced by different cations can be summarized from Figure 6a. The water absorption of the beads in salt solution was significantly lower than that in distilled water. In the swelling of ionic hydrogels, which is a common phenomenon, it is usually due to anion-anion electrostatic repulsion caused by the charge screening effect of cations and osmotic pressure difference between the inside and outside of the hydrogel network [37]. As can be seen from Figure 6a, the swelling ratio of hydrogel beads in different salt solutions were ranked from highest to lowest as KCl > NaCl > MgCl 2 > CaCl 2 > AlCl 3 . This may be due to the complexation of polyvalent metal cations and hydroxyl groups on chitosan and the influence of ionic strength.  Figure 7 demonstrates the effect of the temperature on swelling of chitosan-chlorella hydrogel beads. It is obvious that the swelling degree decreased significantly with the increasing temperature. When the temperature was low (20~40 °C), the swelling rate of the chitosan-chlorella hydrogel beads with high content of Chlorella was significantly higher than that without Chlorella. When the temperature was high (50, 60 °C), the swelling rate of hydrogel beads decreased significantly due to the breakage of hydrogen bonds. At lower temperature, the presence of Chlorella makes it easier for water molecules to diffuse into the hydrogel. Therefore, the swelling ratio of hydrogel beads with high Chlorella content is stronger. When the temperature increased to 60 °C, Chlorella lost its bioactivity at high temperature, resulting in the swelling ratio of the hydrogel beads with high Chlorella content decreasing to the same level as that with low Chlorella content. The presence of Chlorella cells in the hydrogel network enhanced the water retention property to a certain extent, which indicated that Chlorella was feasible as a filling biomaterial. The swelling of chitosan-chlorella hydrogel beads in different concentrations of NaCl, CaCl 2 , and AlCl 3 salt solutions was studied as shown in Figure 6b. Two conclusions were obtained from Figure 6b: First, the swelling ratio of the hydrogel beads decreased with increasing concentrations of saline solution. This phenomenon was attributed to the fact that when the concentration of the salt solution increased, the concentration difference of the ions between the internal and external saline solution of the hydrogel network became smaller and the osmotic pressure difference decreased. The high salt concentration resulted in high external osmotic pressures, and in order to counteract the increase in external osmotic pressure, water molecules diffused from the inside to the outside of the hydrogel network, ultimately leading to a decrease in water uptake at equilibrium [38]. Second, the water absorption ability of hydrogels in various salt solution at the same concentration from high to low was Na + > Ca 2 + > Al 3 + . The data in Table 1 showed that the ionic strength is ordered as Al 3 + > Ca 2 + > Na + under the same concentration of the salt solution. That is, the swelling degree of the hydrogel beads decreased when the ionic strength of the external salt solution increased. The influence of ionic strength on the swelling of hydrogel can be calculated through Flory's equation [39]:

Effect of Various Temperature on Water Absorbency
where S is the degree of swelling, i/V u is the electric charge density of hydrogel, I is the ionic strength of external solution, (1/2 − X i )/V 1 is affinity of the hydrogel and the swelling medium, and V e /V 0 is the cross-link density.
where I is the ionic strength of the saline solution, C i is each ionic concentration of the saline solution, and Z i is the charge number of the corresponding ion.
In order to contrast the sensitivity difference of chitosan-chlorella hydrogel beads to different swelling media, a dimensionless salt sensitivity factor f for 10 mmol·L −1 salt solution was designed and could be calculated by the following formula [40]: where S g and S d are the water absorption in given fluid and in distilled water, respectively. Figure 6c shows the dimensionless salt sensitive factor of chitosan-chlorella hydrogel beads in various salt solutions with the same concentration of 10 mmol/L. The higher the f value becomes, the greater the salt sensitivity is observed, and the lower the swelling degree in the corresponding fluid. As shown in Figure 6c, the f value is related to the charge number of the metal cation. More specifically, the salt sensitivities of different multivalent saline solutions are in the order of monovalent > divalent > trivalent cations. The AlCl 3 solution exhibit the strongest salt sensitivity, and here, the anti-swelling effect of ionic crosslinking plays a more critical role compared to the charge shielding effect. Figure 7 demonstrates the effect of the temperature on swelling of chitosan-chlorella hydrogel beads. It is obvious that the swelling degree decreased significantly with the increasing temperature. When the temperature was low (20~40 • C), the swelling rate of the chitosan-chlorella hydrogel beads with high content of Chlorella was significantly higher than that without Chlorella. When the temperature was high (50, 60 • C), the swelling rate of hydrogel beads decreased significantly due to the breakage of hydrogen bonds. At lower temperature, the presence of Chlorella makes it easier for water molecules to diffuse into the hydrogel. Therefore, the swelling ratio of hydrogel beads with high Chlorella content is stronger. When the temperature increased to 60 • C, Chlorella lost its bioactivity at high temperature, resulting in the swelling ratio of the hydrogel beads with high Chlorella content decreasing to the same level as that with low Chlorella content. The presence of Chlorella cells in the hydrogel network enhanced the water retention property to a certain extent, which indicated that Chlorella was feasible as a filling biomaterial.

Swelling Kinetics in Distilled Water
The time-dependent swelling process of the chitosan-chlorella hydrogel beads with various Chlorella contents in distilled water was given in Figure 8a. The swelling degree of samples increased rapidly at first, and then the rate of increase gradually reduced. The final state of swelling equilibrium was achieved after approximately 50 min. During the swelling process, water needed to continuously overcome the osmotic pressure inside the hydrogel. The higher the difference of osmotic pressure, the faster the water diffused into the hydrogel beads. As the hydrogel beads swelled by water absorption, the osmotic pressure difference between the inside and outside of the beads continuously decreased, and the swelling rate gradually decreased and finally reached equilibrium.
To investigate the swelling kinetics mechanism of hydrogels in distilled water, the pseudo-first-order and pseudo-second-order models were employed to fit the experimental data. The pseudo-first-order swelling kinetic model was based on the assumption that adsorption is controlled by diffusion processes, and the adsorption ratio was dependent on the number of remaining adsorption sites. This swelling kinetic model could be given by the following equation [41]: While the pseudo-second-order kinetics model could be expressed as the following formula: where Se (g/g) is the equilibrium swelling ratio, St (g/g) is the swelling ratio at contact time t (min), and K1 and K2 are the pseudo-first-order rate constant (min −1 ) and the pseudosecond-order rate constant (g·mg −1 ·min −1 ), respectively. As a result, the fitted curves of the swelling process obtained by the pseudo-firstorder and pseudo-second-order kinetic models were presented in Figure 8b,c. The values of K1, K2, Se, and the correlation coefficients (R 2 ) are given in Table 2. The R 2 value of the pseudo-second-order kinetics model was closer to one. Furthermore, the Se value calculated by the pseudo-second-order kinetic model were closer to experimental data than those obtained by the pseudo-first-order model. It means that the pseudo-second-order model fits the data better than the pseudo-first-order model. Thus, this swelling process better fits the pseudo-second-order kinetic model.

Swelling Kinetics in Distilled Water
The time-dependent swelling process of the chitosan-chlorella hydrogel beads with various Chlorella contents in distilled water was given in Figure 8a. The swelling degree of samples increased rapidly at first, and then the rate of increase gradually reduced. The final state of swelling equilibrium was achieved after approximately 50 min. During the swelling process, water needed to continuously overcome the osmotic pressure inside the hydrogel. The higher the difference of osmotic pressure, the faster the water diffused into the hydrogel beads. As the hydrogel beads swelled by water absorption, the osmotic pressure difference between the inside and outside of the beads continuously decreased, and the swelling rate gradually decreased and finally reached equilibrium.   To investigate the swelling kinetics mechanism of hydrogels in distilled water, the pseudo-first-order and pseudo-second-order models were employed to fit the experimental data. The pseudo-first-order swelling kinetic model was based on the assumption that adsorption is controlled by diffusion processes, and the adsorption ratio was dependent on the number of remaining adsorption sites. This swelling kinetic model could be given by the following equation [41]: While the pseudo-second-order kinetics model could be expressed as the following formula: where S e (g/g) is the equilibrium swelling ratio, S t (g/g) is the swelling ratio at contact time t (min), and K 1 and K 2 are the pseudo-first-order rate constant (min −1 ) and the pseudo-second-order rate constant (g·mg −1 ·min −1 ), respectively. As a result, the fitted curves of the swelling process obtained by the pseudo-first-order and pseudo-second-order kinetic models were presented in Figure 8b,c. The values of K 1 , K 2 , S e , and the correlation coefficients (R 2 ) are given in Table 2. The R 2 value of the pseudo-second-order kinetics model was closer to one. Furthermore, the S e value calculated by the pseudo-second-order kinetic model were closer to experimental data than those obtained by the pseudo-first-order model. It means that the pseudo-second-order model fits the data better than the pseudo-first-order model. Thus, this swelling process better fits the pseudo-second-order kinetic model.

Application of Chitosan-Chlorella Hydrogel Beads for Loading and Controlled Release of Humic Acid
Humic substances are widely found in soils and water bodies in nature [42]. Humic acids have the effect of improving soil physicochemical properties and increasing grain yield [43]. In addition, humic acids can prevent nutrient degradation and loss, thereby reducing the use of inorganic fertilizers and improving fertilization efficiency. Actually, humic acids express as mixture of multiple dibasic acids, and their compositions are relatively complex: They can be divided into humic acids (HA), fluvic acids (FA), and humins based their solubility in acid-base solution. HA is soluble in base but insoluble in acid, FA is soluble in both base and acid, and humins are soluble in dilute base but insoluble in acid and water [44]. A simple and controllable use method has a significant role in agricultural applications of humic acids. Therefore, we chose humic acid as a model fertilizer to study the controlled release ability of hydrogel beads. For this purpose, the pH-and temperature-responsive chitosan-chlorella hydrogel beads with 40 wt% Chlorella content were selected as the matrix material, and the loading and slow-release efficiency for humic acid were investigated. Figure 9a shows the influence of pH on the loading efficiency of humic acid in hydrogel beads with 40 wt% Chlorella content. The loading of humic acid gradually reached equilibrium with the swelling process of the beads. When the solution pH was 4, the loading efficiency of humic acid was higher, the maximum loading rate was 83.6%, and it decreased obviously as the pH increased from 4 to 10. During the loading process, the interaction between the chitosan-chlorella hydrogel beads and humic acid was mainly the complexation of protonated amino groups on chitosan with carboxyl or phenolic groups in humic acid [45]. In lower pH solution, more amino groups on chitosan were protonated, while protonated amino groups can combine with -COOH in humic acid, thus greater adsorption was obtained. Moreover, from the point of view of electrostatic action, the protonated amino groups on chitosan express electrostatic attraction to humic acid at pH < 6. However, when the pH was 2, the chitosan-chlorella hydrogel beads were corroded by acidic solution, which resulted in the destruction of the crosslinked network structure and lower loading efficiency of humic acid.

Conclusions
In summary, we successfully prepared eco-friendly chitosan-chlorella hydrogel beads with various Chlorella contents in a facile and economical method and explored the synthesis mechanism in detail. The introduction of Chlorella into chitosan matrix brought  Figure 9b shows the release efficiency of the beads loaded with humic acid with various pH. The results indicated that the slow-release of humic acid was a pH-responsive process. The slow-release efficiency increased at high pH, and the release reached a balance of 35.8%, 54.2%, and 73.2% at pH 4, 6, and 8, respectively. With high pH of the in vitro solution, the humic acids were released as more protonated amino groups on chitosan were deprotonated. Meanwhile the slow-release is also limited by the hydrogen bonding effect between chitosan and humic acid. Specifically, hydrogen bonds between amino groups on chitosan and carboxyl or phenolic groups in humic acid prevented the humic acid from releasing. There was also a portion of humic acids trapped in the cross-linked network that could not be released, resulting in a slow-release efficiency below 100%.

pH-Responsive Release Behavior of Hydrogel Beads
Considering that the release process was a pH-dependent behavior, the pH-responsive controlled release of the loaded humic acid was investigated by changing the pH of external solution. The beads containing humic acid were first placed into distilled water at pH 4 for 120 min, and then the pH was changed to 8. The results of controlled release efficiency are shown in Figure 9c. The release rate was slower at pH 4, and the release efficiency reached 20% at 120 min. When pH was changed to 8, a dramatic step change in the release efficiency of humic acid release occurred, and more time was required to reach equilibrium, with the release efficiency eventually reaching approximately 80%. In summary, the pH-responsive controlled release of humic acid demonstrated that the chitosan-chlorella hydrogel beads could respond to the pH changes in the external solution, which indicated the potential of chitosan-chlorella hydrogel beads as carrier materials for controlled release.

Temperature-Responsive Release Behavior of Hydrogel Beads
In addition to pH-responsive capacity, the sensitivity of chitosan-chlorella hydrogel beads to temperature allows for temperature-responsive controlled release. Figure 9d shows the cumulative release of humic acid at various temperatures (20 • C, 30 • C, and 40 • C). The cumulative release percentage decreased with increasing temperature, and the fastest release rate was achieved at 20 • C with the final cumulative release percentage of 66.4%, which was higher than those at 20 • C and 30 • C under the same conditions. It may be attributed to the enhanced swelling properties, where hydrogel beads have better water absorption properties at lower temperatures and humic acids are easier to release into the medium from the expanded cross-linked network.

Conclusions
In summary, we successfully prepared eco-friendly chitosan-chlorella hydrogel beads with various Chlorella contents in a facile and economical method and explored the synthesis mechanism in detail. The introduction of Chlorella into chitosan matrix brought enhanced mechanical stability and better swelling properties to the chitosan-chlorella hydrogel beads. The enhanced mechanical stability allowed the hydrogel beads to retain more water when facing ultrasound or centrifugation. Chitosan-chlorella hydrogel beads were obviously responsive to the external factors such as the pH value, saline solution concentration and temperature, specifically in the form of differences in swelling degree. The resultant hydrogel beads with 40 wt% Chlorella content reached the maximum swelling degree of 42.92 g/g in distilled water, and swelling behavior was better fitted to the pseudosecond-order kinetic model. In addition, chitosan-chlorella hydrogel beads are stimulated responsive to pH and temperature during slow release and can better cope with the complex environment of practical agricultural controlled release fertilizer applications.