Bioremediation of Raw Landﬁll Leachate Using Galdieria sulphuraria : An Algal-Based System for Landﬁll Leachate Treatment

: This study aims to evaluate the potential of using a thermophilic acidophilic red alga, Galdieria sulphuraria for effective on-site treatment of municipal landﬁll leachate (LL). This study focused on evaluating the effects of LL dilution, nitrogen loading, and initial algal biomass density on the overall treatment efﬁciency, and evaluated the long-term performance of the system using 5-day growth cycles. This study conﬁrmed that optimal conditions for G. sulphuraria biomass production are 20% strength LL, a lower initial biomass concentration of 0.25 g L − 1 , and the addition of N at twice the level of initial media. Furthermore, the results indicated G. sulphuraria ’s ability to grow in elevated NH 4 -N concentration (>950 mg L − 1 ) and provide nitrogen removal rates of up to 40 mg L − 1 d − 1 . In addition, the long-term running experiment showed that the proposed algal-based system could be applied in semi-continuous mode to achieve bioremediation. Overall, the results obtained from this study can be used to develop the necessary process parameters to implement large-scale algal-based systems for landﬁll leachate treatment.


Introduction
Released over time from municipal landfills, landfill leachate (LL) is an aqueous waste stream containing dissolved organic matter, inorganic macro components, heavy metals, and xenobiotic organic components. LL requires considerable treatment before it can be released into the environment and can pose a significant risk to the groundwater, surface water, and adjacent ecosystems if not treated adequately [1]. In the United States, landfill operations are mandated to collect leachate under the Resource Conservation and Recovery Act of 1986 and treat the collected leachate before releasing it to the environment in accordance with the Effluent Guideline and Standards [2]. The most common method of handling and treating LL is to transfer it to a municipal wastewater treatment plant (MWTP). In MWTPs the LL is co-treated with the municipal wastewater. However, there is a growing demand to explore technologies to treat the leachate on-site, to avoid regulatory and permitting issues arising from co-treatment with municipal wastewaters. Given the renewed paradigm shift on viewing the waste as a resource, there have been attempts to utilize the embedded chemical energy in the leachate to produce useful by-products to improve the sustainability of the treatment technology while achieving the required level of treatment [2,3]. This warrants the need for developing technologies to treat LL on-site and independently of municipal wastewater [4]. Multiple studies have demonstrated the feasibility of using an algal-based system to treat LL [3,5,6]. These algal-based bioremediation systems are often considered as an alternative to traditional LL treatment processes. These algal-based systems provide valuable environmental services by removing ammoniacal nitrogen and phosphate from the LL, and through carbon sequestration. To further improve the economics of the process, the produced algal biomass can be used for the production of biofuels, livestock feeds, and value-added products [7]. Several recent studies evaluated the potential of on-site treatment of LL using multiple algal strains [2,8,9]. Hydraulic retention time and nutrient removal rates play a vital role on the achievement of an algal-based LL in an industrial-scale application. However, most published studies utilized green algal strains such as Chlorella sp. and Chlamydomonas sp. [10][11][12]. These strains often require neutral pH conditions for optimized growth. This leads to a plethora of culture stability issues such as invasion of native species and subsequent competition for resources, thus reducing bioremediation rates. Moreover, past studies used a higher dilution of LL to reduce the inherent toxicity, resulting in lower nutrient removal rates and longer hydraulic retention times [4,13,14].
Galdieria sulphuraria 5587 is a thermophilic mixotrophic alga that thrives between pH 0.5-4.0 and temperatures up to 56 • C [15]. G. sulphuraria's ability to withstand, and grow in, extreme conditions enables the strain to set itself apart from other algal strains. Specifically, the low pH and high-temperature culture environment enable the deactivation of pathogens and competitive algal species that exist in the natural environment [16]. Furthermore, the mixotrophic nature of G. sulphuraria enables higher productivity through potential carbon supplementation, and helps to reduce the hydraulic retention times to achieve required levels of bioremediation [17,18]. Several past studies demonstrated G. sulphuraria's ability to grow in municipal wastewater for bioremediation applications [15,17]. In our previous study [2], we cultivated G. sulphuraria in different strengths of LL and compared the growth rates and nutrient removal with that in a standard growth media. These preliminary studies were conducted for 14 days using 50 mL photobioreactors in batch mode. These results demonstrated that G. sulphuraria could grow in LL with a maximum of 5-fold dilution and achieve comparable nutrient removal rates. Our past study provided proof-of-concept type data for the applicability of using G. sulphuraria for LL treatment [2]. However, the real-world implementation of this algal-based system for the LL treatment warrants more studies on process optimization through long-term growth trials. Therefore, to facilitate informed decision-making on this proposed algal-based LL treatment system, this study evaluates the effects of LL dilution, nitrogen loading, and initial algal biomass density on the overall treatment efficiency. In the first part of the study, we used a 2 3 full factorial experimental design to evaluate the system's performance. Then, in the second part of the study, we evaluated the system's performance in long-term experiments using 5-day cycles. Overall, this study aims to optimize the proposed LL treatment system's performance by optimizing the process parameters.

Culturing of G. sulphuraria and Landfill Leachate Collection
The isolates of red algae, G. sulphuraria CCMEE 5587.1, obtained from the Culture Collection of Microorganisms from Extreme Environments (University of Oregon), were used in this study. G. sulphuraria was grown in an incubator (Percival, IA, USA) at 42 • C with 24 h of continuous illumination (4000 lux) in Cyanidium media (CM) [2,19]. The cultures from the collection were streaked onto agar plates, and single colonies were picked from the culture plates to start axenic cultures on the CM, then the volume was scaled up to 1 L Erlenmeyer flasks. The CO 2 concentration inside the incubator was maintained at 3%. The following macro and micro level constituents were used to prepare the CM: (NH 4 ) 2 SO 4 , Water 2022, 14, 2389 3 of 14 1.32 g L −1 ; KH 2 PO 4 , 0.27 g L −1 ; NaCl, 0.12 g L −1 ; MgSO 4 ·7H 2 O, 0.25 g L −1 ; CaCl 2 ·2H 2 O, 0.07 g L −1 ; Nitch's trace element solution, 0.5 mL L −1 ; and FeCl 3 (solution = 0.29 g L −1 ), 1.0 mL L −1 . The pH of the media was adjusted to 2.5 with 10 N H 2 SO 4 . The LL used for this research was collected from St. John the Baptist Wastewater Treatment System (LaPlace, LA, USA) and stored in a standard refrigerator at 4 • C. The characteristics of the LL used in this study are summarized in Table 1. The parameters listed in Table 1 were measured using the methods listed in Pan et al. (2021) [2]. The goal of Experiment 1 was to evaluate the effect of influent LL characteristics, namely: LL loadings, initial nitrogen concentration, and initial biomass concentration on nutrient removal from the LL and algal biomass growth. A 2 3 full factorial design experiment was used in Experiment 1. For this experiment, two different initial LL media compositions (20% LL and 40% LL), two different initial ammoniacal nitrogen concentrations (1N and 2N), and two different initial algal biomass densities (0.25 g L −1 and 0.75 g L −1 ) were used. These two initial biomass densities were selected to evaluate the effect of initial biomass density on the required hydraulic retention time of the system. The percentage of each LL composition represents the volume ratio of LL to the entire media, and deionized water was used to dilute the LL. For the ammoniacal nitrogen, two different concentrations were used. Set 1N represents the original concentration of ammoniacal nitrogen present in the LL after the corresponding dilution (20% and 40%), whereas 2N represents additional ammoniacal nitrogen supplementation using (NH 4 ) 2 SO 4 for doubling the initial ammoniacal nitrogen present in the LL after the corresponding dilution. Based on our previous study, Pan et al. (2021) [2], an N/P ratio of 25:1 was used in this experiment to calculate the supplemental phosphate concentrations, and subsequently, phosphate was added to the media compositions using KH 2 PO 4 . In addition, all media compositions were supplemented with CM constituents except for (NH 4 ) 2 SO 4 , and KH 2 PO 4 . The final pH of each media composition was adjusted to 2.5 using 10 N H 2 SO 4 . The complete experimental design is shown in Table 2.
The inoculum algae for the experiment were grown in 1 L Erlenmeyer flasks, as described in Section 2.1. At the beginning of the experiment, the inoculum was centrifuged at 2000× g for 10 min at 25 • C (accuSpin 400 centrifuge, Fisher Scientific, Waltham, MA, USA) and the algae pellets were re-suspended in the eight media compositions. Each media composition was prepared in triplicate in 125 mL Erlenmeyer flasks with a working volume of 50 mL. Then the flasks were placed on a platform shaker (Innova 2000-New Brunswick Platform Shaker, Eppendorf, Enfield, CT, USA) inside the incubator with the same culture growth conditions as mentioned in Section 2.1. The algal biomass production was quantified every day. For nutrient analysis, 1 mL samples were drawn from each flask on days 3, 6, 10, and 14. The samples were centrifuged for 10 min at 2000× g followed by the supernatants being transferred and stored in a refrigerator at 4 • C for further analyses. Experiment 2 was designed to evaluate the performance of the proposed system in a long-term experiment. Most of the previous experiments (including Experiment 1 in this paper) were conducted in batch mode. This experiment aimed to evaluate the nutrient removal and biomass growth rate in a semi-continuous mode. Experiment 2 was conducted for 25 days using 5-day cycles (a total of 5 cycles), using 1 L tubular bubbling column bioreactors with a working volume of 500 mL. The experiment was conducted using five reactors (n = 5). Based on the results obtained from Pan et al. (2021) [2] and Experiment 1, 20% LL with an N/P ratio of 25:1 was selected as the initial growth media for this long-term experiment. After drawing samples for biomass, nutrient, and metal analyses at the end of each growth cycle (after 5 days), all the treated LL media from all 5 reactors were mixed together. Then, 500 mL (1/5th) of the treated sample was mixed with 500 mL (1/5th) of fresh LL and 1500 mL (3/5th) of deionized water to prepare a total of 2500 mL growth media for the next cycle. This process mimicked a 5-fold dilution of the biomass density at the end of each cycle, replacing 1/5th of the total volume of the reactors with fresh LL. Then, the total 2500 mL of the newly prepared media was equally divided and added to the 5 reactors for the next cycle.

Sample Analysis
A Hanna pH meter (HI 5522, Hanna, Woonsocket, RI, USA) was used for pH and conductivity measurements. Ammoniacal nitrogen, nitrate nitrogen, nitrite nitrogen, total nitrogen, total phosphorus, and ortho-phosphorus (phosphate) were determined using a HACH DR 3900 (HACH, Loveland, CO, USA) spectrophotometer, with different standard HACH vials or powder for corresponding parameters and measurement range. Metal elements were analyzed using inductively coupled plasma optical emission spectrometry iCAP 7000 (ICP-OES) (Thermo Fisher Scientific, Waltham, MA, USA). Biomass density was quantified by measuring the optical density (OD) at the 750 nm wavelength using a spectrophotometer (HACH, Colorado, USA). The biomass density was evaluated in terms of 'ash-free dry weight' (g L −1 ), which was correlated to OD at 750 nm by the following equation [2]: All the experiments and analytical measurements were carried out in triplicate. The averaged data were presented with error bars equal to one standard deviation. Microsoft Excel software (Version 16.0, Redmond, WA, USA) was used for the standard deviation calculations.

Effects of Experimental Media Compositions on the Growth of G. sulphuraria
Final biomass density and growth rate of G. sulphuraria, cultured in different LL media compositions, are shown in Figure 1, for 14 days. This figure shows that G. sulphuraria grew in all of the eight different LL media and achieved a final biomass density within a range of 1.36-3.15 g L −1 . The highest and second-highest final biomass densities (i.e., 3.15 and 3.09 g L −1 ) were observed in two 20% LL-HB treatments, with three times more initial algal biomass densities than the LB treatments (0.75 vs. 0.25 g L −1 ). In contrast, the lowest final biomass density was observed in 40% LL-LB-1N. G. sulphuraria started to grow rapidly in four 20% LL treatments from the initial experimental days, whereas 40% LL treatment showed a lag phase of two days. It is evident that high concentration LL has higher toxicity to algae. This finding is corroborated by the results observed in the previous study by the same authors [2]. Pan et al. (2021) [2] showed that the growth of G. sulphuraria was inhibited at LL concentrations above 40%. Furthermore, the inhibitory effect of LL has been previously reported for organisms such as microalgae, duckweed, bacteria, and protozoan [20][21][22]. Higher heavy metal concentrations in the LL are considered the main inhibitors of algal growth [23].   Table 1 for LL media composition details.

Effects of Experimental Media Compositions on Nitrogen Removal
The removal of ammoniacal nitrogen by G. sulphuraria cultured in differen compositions over 14 days are presented in   Table 1 for LL media composition details.
The growth rates for all eight experiments ranged from 0.074 to 0.19 g L −1 d −1 (Figure 1). However, the growth rates in all four 20% LL treatments were much higher than the corresponding 40% LL treatment. For instance, the growth rate in 20% LL-LB-2N was 0.19 g L −1 d −1 , which was approximately two times higher than in 40% LL-LB-2N (i.e., 0.098 g L −1 d −1 ). Therefore, our current findings reveal that 40% LL had a significant negative effect on G. sulphuraria's growth rate and, consequently, its biomass production (R 2 = 0.92, 0.934; p < 0.05; Table 3). Lin et al. (2007) [10] conducted algal growth experiments at various LL concentrations of 10%, 30%, 50%, 80% and 100% using Chlorella pyrenoidosa and Chlamydonomas snowiae, and reported that 10% leachate could support algal growth, whereas a higher concentration of leachate could significantly reduce the algal growth rate.

Effects of Experimental Media Compositions on Nitrogen Removal
The removal of ammoniacal nitrogen by G. sulphuraria cultured in different LL media compositions over 14 days are presented in Figure 2. These findings indicate that the high LL loading, and double N concentration caused a significant decrease in removal efficiency (p < 0.05; Table 3). However, high initial biomass increased removal efficiency in most cases (p < 0.05; Table 3). For example, the removal efficiency increased from 52.3% to 83.5% as the biomass concentration increased in the 40% LL-1N group. The N removal rates, presented in Figure 2, were calculated from N concentrations' respective linear decline zones. N removal rates for all media compositions were in the range of 22.05 to 39.76 mg L −1 d −1 . These results were competitive with the studies conducted in the past with other algal strains, even for some strains known to be ammoniacal nitrogen tolerant algal strains [2,10]. The removal rates in past studies ranged from 2.0 to 74.6 mg L −1 d −1 [10][11][12]24]. Initial double N concentration increased N removal rate in all media compositions except 20% LL-HB group. Other studies have similarly reported that a higher initial N concentration increases N removal rate [5,9]. In   [25], the nitrogen removal rate increased from 5.71 to 11.29 mg L −1 d −1 by doubling initial N concentration from 40 to 80 mg L −1 . Additionally, a high initial biomass concentration showed significantly higher N removal rate in all N concentration levels (i.e., 200,~400 and~900 mg L −1 ) (p = 0.044 < 0.05; Table 3). However, this finding was different from   [26], who used the same algae strain to treat municipal wastewater; the study found no change in N removal rate among initial biomass density with 0.1, 0.2 and 0.4 g L −1 . Jia and Yuan (2018) [27] explored the effects of ammonium concentration, algae biomass, and light intensity on ammonium removal using algaebacteria consortia and found no promotion of N removal rate when increasing initial biomass density, represented as optical density, from 1.0 to 1.4. The study inferred the possible reason being the relative low light intensity (~1000 lux) used for the experiment, which could limit the photosynthesis of algae. The light intensity of 4000 lux was used in the current study, which might be one possible explanation for the increased ammoniacal nitrogen removal observed in this study. Furthermore, the mass densities used in this study were comparatively higher than the biomass densities used in the previous two studies (0.4 g L −1 vs. 0.75 g L −1 ). However, further studies are needed to validate this claim. observed in this study. Furthermore, the mass densities used in this study w atively higher than the biomass densities used in the previous two studies 0.75 g L −1 ). However, further studies are needed to validate this claim.  Table 1 for LL media compo

Effects of Experimental Media Compositions on Phosphate Removal
The removal of phosphate by G. sulphuraria cultured in different LL me tions for 14 days is presented in Figure 3. Phosphate concentrations rapid with time in all 20% LL media, while this decline was relatively slow in 40% the first 3 days. Moreover, the PO4-P (hereafter referred to as P) concentratio 20% LL groups at day 14 were close to 0 mg L −1 with more than 96% P remova 40% LL media retained relatively more P at the end of the experiment. Percen results for phosphate were 97.6%, 98%, 96.4%, 97.6%, 68.5%, 82.4%, 93.2% an 20% LL-LB-1N, 20% LL-LB-2N, 20% LL-HB-1N, 20% LL-HB-2N, 40% LL-LL-LB-2N, 40% LL-HB-1N, and 40% LL-HB-2N, respectively. This finding i 40% LL had significantly lower P removal efficiency than 20% LL groups (p 3). For instance, P removal efficiency decreased from 97.6% to 68.5% for L However, double N concentration increased the phosphate removal efficienc ple, P removal efficiency increased from 68.5% to 82.4% for 40% LL-LB grou more, high initial biomass significantly increased P removal efficiency in mo 0.05; Table 3). Similar to ammoniacal nitrogen removal rate calculation, a l zone of P concentration was used to calculate P removal rates. P remova groups were in the range of 1.16 to 2.00 mg L −1 d −1 (Figure 3). These rates were that of the many algae species that have been previously used to treat LL [ removal rate in the 40% LL-HB-2N group was the highest among all group 2 mg L −1 d −1 , when initial ammonia N concentration was as high as ~1000 mg N concentration increased P removal rate in all compared pairs. Additionally biomass concentration showed higher P removal rate in all compared pair 20% LL-2N groups (R 2 = 0.645, p < 0.05; Table 3).  Table 1 for LL media composition details.

Effects of Experimental Media Compositions on Phosphate Removal
The removal of phosphate by G. sulphuraria cultured in different LL media compositions for 14 days is presented in Figure 3. Phosphate concentrations rapidly decreased with time in all 20% LL media, while this decline was relatively slow in 40% LL media in the first 3 days. Moreover, the PO 4 -P (hereafter referred to as P) concentrations in all four 20% LL groups at day 14 were close to 0 mg L −1 with more than 96% P removal. In contrast, 40% LL media retained relatively more P at the end of the experiment. Percentage removal results for phosphate were 97.6%, 98%, 96.4%, 97.6%, 68.5%, 82.4%, 93.2% and 93.2%, for 20% LL-LB-1N, 20% LL-LB-2N, 20% LL-HB-1N, 20% LL-HB-2N, 40% LL-LB-1N, 40% LL-LB-2N, 40% LL-HB-1N, and 40% LL-HB-2N, respectively. This finding indicates that 40% LL had significantly lower P removal efficiency than 20% LL groups (p < 0.05; Table 3). For instance, P removal efficiency decreased from 97.6% to 68.5% for LB-1N group. However, double N concentration increased the phosphate removal efficiency. For example, P removal efficiency increased from 68.5% to 82.4% for 40% LL-LB groups. Furthermore, high initial biomass significantly increased P removal efficiency in most cases (p < 0.05; Table 3). Similar to ammoniacal nitrogen removal rate calculation, a linear decline zone of P concentration was used to calculate P removal rates. P removal rates of all groups were in the range of 1.16 to 2.00 mg L −1 d −1 (Figure 3). These rates were higher than that of the many algae species that have been previously used to treat LL [12,28]. The P removal rate in the 40% LL-HB-2N group was the highest among all groups, which was 2 mg L −1 d −1 , when initial ammonia N concentration was as high as~1000 mg L −1 . Double N concentration increased P removal rate in all compared pairs. Additionally, high initial biomass concentration showed higher P removal rate in all compared pairs, except for 20% LL-2N groups (R 2 = 0.645, p < 0.05; Table 3).   Table 1 for LL media compositio  Figure 4 shows the growth profile, NH4-N concentration and removal effi PO4-P concentration and removal efficiency by G. sulphuraria in 20% LL on a  Table 1 for LL media composition details. Figure 4 shows the growth profile, NH 4 -N concentration and removal efficiency, and PO 4 -P concentration and removal efficiency by G. sulphuraria in 20% LL on a long-term running experiment. This experiment lasted for 25 days and consisted of five growth cycles with each cycle running for 5 days. Biomass concentration, NH 4 -N and PO 4 -P concentration were~0.5 g L −1 ,~208 mg L −1 and~8.4 mg L −1 , respectively, at the beginning of the first cycle. The algal biomass concentration, presented as ash-free dry weight in Figure 4a, increased from the beginning until each cycle's end.

Long-Term Running Performance of Algal-Based LL Treatment System
At the end of each cycle, the highest biomass concentrations were 4.34, 2.88, 2.29, 2.37 and 2.22 g L −1 , respectively. The calculated growth rate for the five cycles were 0.768, 0.414, 0.347, 0.397 and 0.356 g L −1 d −1 , respectively. The highest biomass density was observed at the end of the first growth cycle. However, after the first two cycles, the growth performance tended to be stable in the subsequent cycles. In most semi-continuous algal experiments, the growth systems need time to stabilize biomass productivity. The initial elevated growth rates observed in the first two cycles can be attributed to the potential carryover of macro/micro-nutrients from the seed cultures. The mean final biomass density and growth rate of the last three cycles were 2.29 ± 0.072 g L −1 and 0.37 ± 0.027 g L −1 d −1 . Similar to the growth performance, cycles 1 and 2 showed the highest N removal performance, which further decreased in the next cycles (Figure 4b). The N % removal efficiencies for the five cycles were 99.8%, 99.9%, 91%, 82% and 71.4%, respectively. However, no completely stable performance was observed in subsequent cycles. This instability indicates that a higher cycle number would be required to attain consistent NH 4 -N removal. Nevertheless, nearly complete P removal was observed as presented in Figure 4c. G. sulphuraria achieved almost 100% P removal efficiencies in all five cycles. This finding indicates that the N/P mass ratio of 25:1 is ideal for the long-term running of a G. sulphuraria-based LL treatment system. Additionally, in terms of P removal, this ratio could meet discharge standards after wastewater treatment. Table 4 shows the periodical change of pH, conductivity, and selected metal concentrations in the LL used in this experiment. The results indicate that the pH decreased from 2.5 to below 2.0 during each cycle. Although pH was adjusted to~2.5 at the beginning of each cycle, the growth of G. sulphuraria could acidify the LL media. It has previously been reported that G. sulphuraria can acidify the growth media during the growth period. For example, Oesterhelt et al. (2007) [29] reported the acidification of growth media by G. sulphuraria from a starting pH 8 to pH 3 after 25 days. However, there were no significant changes in metal ion concentration for the presented metals between the cycles or during each cycle. A small amount of removal was observed for metals such as Mg, As, and Ca. About 50% Mn was removed from the LL in cycle 2 to cycle 5. The increase in concentrations of some metals such as Ba, Fe, and Hg were noted. Further targeted research efforts are needed to evaluate the impact of the metals present in the growth system. Nevertheless, nearly complete P removal was observed as presented in Figure 4c. G. sulphuraria achieved almost 100% P removal efficiencies in all five cycles. This finding indicates that the N/P mass ratio of 25:1 is ideal for the long-term running of a G. sulphurariabased LL treatment system. Additionally, in terms of P removal, this ratio could meet discharge standards after wastewater treatment.   Table 4 shows the periodical change of pH, conductivity, and selected metal concentrations in the LL used in this experiment. The results indicate that the pH decreased from

Comparison between the Current Study and the Literature Reported for Long-Term Running
Comparison between this study and other literature studies in terms of nutrient removal, algal biomass production, algae species, and hydraulic retention time (HRT), for long-term LL treatment are shown in Table 5. The majority of existing studies used an algaebacteria consortium for long-term LL treatment, except for Dogaris et al. (2019) [6], and this study. The initial concentration of NH 4 -N in this study was~244 mg L −1 , which was higher than most of the other studies with an average of 132.4 mg L −1 , excluding Martins et al. (2013) [9], in which four different ponds were used to treat the LL in a continuous feeding mode. Meanwhile, highest average N and P removal rates were obtained in this study with 43.7 and 3.99 mg L −1 d −1 , respectively, which were much higher than the previously reported values (0.88-14.36 mg L −1 d −1 for N, and 0.51-0.97 mg L −1 d −1 for P). In addition, a comparable average final biomass density (2.82 g L −1 ) and highest growth rate (0.46 g L −1 d −1 ) in the current study show a promising scale-up potential for biomass production. Moreover, the current study had the shortest HRT (5 days), which indicates a shorter treatment time and thus improves the overall sustainability of the large-scale LL treatment systems.

Limitations and Practical Implications
The results obtained from this study and our previous study [2] demonstrate the potential of using G. sulphuraria for the bioremediation of LL. These studies reiterated the need for a 5-fold dilution of LL to achieve comparable nutrient removal rates. Both studies were conducted in small-scale reactors and in laboratory conditions. However, algal-based systems' bioremediation potential often relies on environmental conditions such as water temperature, dissolved oxygen concentration, and daylight and dark times [13]. In addition, reactor scale plays a vital role in the system's performance. Sniffen et al. (2017) [13] observed a 20% reduction in nitrogen removal rates when comparing the performance of 1000 L ponds to 250 mL flask growth reactors. Therefore, one of the main limitations of the current study is the limited usefulness of the data obtained. The performance obtained in this study can be used only as a proof of concept for the applicability of G. sulphuraria for the bioremediation of LL. Therefore, using large-scale reactors (ponds or reactors in sizes comparable to commercial applications) is likely necessary to produce performance data, which can be used in predictive studies to evaluate the real-life application of this system.

Conclusions
This study confirmed that G. sulphuraria can grow in 20% LL and achieve higher final biomass density and nutrient removal efficiencies than 40% LL composition. This study confirmed that optimal conditions for G. sulphuraria biomass production are 20% LL, with a lower initial biomass concentration of 0.25 g L −1 , and with the addition of N at twice the level of the initial media. It confirmed that the optimal conditions for both nitrogen removal efficiency and removal rate are 20% LL, with a higher initial biomass concentration of 0.75 g L −1 and baseline N concentration. Furthermore, the results indicated G. sulphuraria's ability to grow in elevated NH 4 -N concentration (>950 mg L −1 ) and provide up to 40 mg L −1 d −1 of N removal rates. The results obtained for the long-term running experiment indicated that stable nutrient removal and biomass production can be achieved using a semi-continuous mode operation of the proposed algal-based system. Overall, the results obtained from this study can be used to develop the necessary process parameters to implement a large-scale algal-based system for LL treatment.

Data Availability Statement:
The data presented in this study are available on request from the corresponding authors. The data are not publicly available due to the continuation of a follow-up study by the authors.