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Open AccessArticle

A Morphological and Molecular Analysis of a Bloom of the Filamentous Green Alga Pithophora

1
Department of Biological and Environmental Sciences, Georgia College and State University, Milledgeville, GA 31061, USA
2
US AmeriCorps, Conservation Minnesota and Iowa, 60 Plato Blvd, Saint Paul, MN 55107, USA
3
Oak Ridge Institute for Science and Education, Agricultural Research Services, U.S. Department of Agriculture, 600 E. Greenfield Ave., Milwaukee, WI 53204, USA
4
Department of Natural Sciences, The University of Virginia’s College at Wise, 1 College Ave., Wise, VA 24293, USA
*
Author to whom correspondence should be addressed.
Academic Editor: John R. Beaver
Water 2021, 13(6), 760; https://doi.org/10.3390/w13060760
Received: 25 January 2021 / Revised: 4 March 2021 / Accepted: 6 March 2021 / Published: 11 March 2021
Filamentous representatives of Cladophorales (Chlorophyta) are major contributors to algal biomass of littoral communities. In the present study, community analysis of a reported bloom in the Ogeechee River in Georgia provided an opportunity to combine morphological and genetic analyses with ecological information related to an understudied nuisance alga. A polyphasic approach of incorporating genotypic and phenotypic methods led to the identification of the algal community as Pithophora roettleri (Roth) Wittrock. Morphological analysis showed a monospecific community based on the average length and diameter of the heterosporous intercalary and terminal akinetes, along with the diameter of the principal filaments. Single-gene and concatenated-gene phylogenetic analyses of the LSU (28S rRNA) and SSU (18S rRNA) markers further confirmed this species identification. In this study, we conducted a morphological treatment of P. roettleri, produced 17 novel gene sequences, and produced a new, schematic diagram illustrating the four steps of the asexual reproduction of an intercalary akinete. Morphological characteristics, like the position and shape of akinetes documented here, and the availability of genetic sequences can improve identification and further ecological understanding of filamentous green algae. Flowing mats of P. roettleri, like those observed in this study, can lower light availability for other biota and structurally alter the habitat. View Full-Text
Keywords: Pithophora taxonomy; biology; life cycle; morphological and molecular identification Pithophora taxonomy; biology; life cycle; morphological and molecular identification
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MDPI and ACS Style

Lor, B.; Zohn, M.; Meade, M.J.; Cahoon, A.B.; Manoylov, K.M. A Morphological and Molecular Analysis of a Bloom of the Filamentous Green Alga Pithophora. Water 2021, 13, 760. https://doi.org/10.3390/w13060760

AMA Style

Lor B, Zohn M, Meade MJ, Cahoon AB, Manoylov KM. A Morphological and Molecular Analysis of a Bloom of the Filamentous Green Alga Pithophora. Water. 2021; 13(6):760. https://doi.org/10.3390/w13060760

Chicago/Turabian Style

Lor, Blia; Zohn, Merry; Meade, Marcus J.; Cahoon, A. B.; Manoylov, Kalina M. 2021. "A Morphological and Molecular Analysis of a Bloom of the Filamentous Green Alga Pithophora" Water 13, no. 6: 760. https://doi.org/10.3390/w13060760

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