Hydrogenotrophic Denitrification of Groundwater Using a Simplified Reactor for Drinking Water: A Case Study in the Kathmandu Valley, Nepal

High nitrate-nitrogen (NO3−–N) content is a typical feature of groundwater, which is the primary water source in the Kathmandu Valley, Nepal. Considering the Kathmandu Valley’s current problem of water scarcity, a user-friendly system for removing NO3−–N from groundwater is promptly desired. In this study, a simplified hydrogenotrophic denitrification (HD) reactor was developed for the Kathmandu Valley, and its effectiveness was evaluated by its ability to treat raw groundwater. The reactor operated for 157 days and showed stability and robustness. It had an average nitrogen removal efficiency of 80.9 ± 16.1%, and its nitrogen loading rate and nitrogen removal rate varied from 23.8 to 92.3 g–N/(m3∙d) and from 18.3 to 73.7 g–N/(m3∙d), respectively. Compared to previous HD reactors, this simplified HD reactor is a more user-friendly option for the Kathmandu Valley, as most of the materials used for the reactor were locally available and require less maintenance. The reactor is recommended for groundwater treatment at the household level. It has a current treatment capacity of 40 L/d, which can fulfill the daily requirements for drinking and cooking water in a household with 4–5 people.


Introduction
Drinking water is indispensable to human life, and the United Nations Sustainable Development Goal 6 (SDG) targets the securement of universal and equitable access to safe and affordable drinking water for all people [1]. The Kathmandu Valley includes Kathmandu, the capital and the largest city of Nepal, and it is facing water shortages due to the rapidly expanding population [2] and continued urbanization [3]. There is a considerable discrepancy between the water demand and the water supply in the Kathmandu Valley (the total water demand is 430 million liters per day (MLD), but the water supply averages only 103 MLD) [4]. To address the deficit in water supply, the locals have been compelled to find alternative water sources [5][6][7][8][9][10]. Among them, groundwater has been fill the gap in knowledge between laboratory and practical applications. In this research, groundwater from the Jwagal area located in the Kathmandu Valley, Nepal, where the groundwater is highly contaminated by NH 4 + -N, at levels up to 60 mg-N/L [11], was selected for a case study. The simplified HD reactor was developed using as many locally available materials as possible and installed after a nitrification system, in order to eliminate the NO 3 − -N generated during the nitrification process. This research was designed to support future efforts for deeper insight into the simplified HD reactor, which could potentially have important implications for the application of this technology in developing countries.

Description of the Study Area
The Kathmandu Valley is situated between the latitudes 27 • 32 13 and 27 • 49 10 N, and the longitudes 85 • 11 31 and 85 • 31 38 E. The study area, Jwagal, is located in the middle of the Kathmandu Valley ( Figure 1). In Jwagal, Kathmandu Upatyaka Khanepani Limited (KUKL) operates a water treatment unit to extract deep groundwater for intermittent distribution. The extracted groundwater is treated by aeration, coagulation and flocculation, sedimentation, sand filtration, and chlorination, as shown in Figure 2, before distribution to households.
information is available on the application of HD for raw groundwater treatment and the behavior of HD systems under on-site conditions. Therefore, the objective of this research is to develop a simplified, user-friendly HD reactor for people in the Kathmandu Valley, Nepal, and use it for raw groundwater treatment to evaluate its effectiveness, understand its behavior under on-site conditions, and fill the gap in knowledge between laboratory and practical applications. In this research, groundwater from the Jwagal area located in the Kathmandu Valley, Nepal, where the groundwater is highly contaminated by NH4 + -N, at levels up to 60 mg-N/L [11], was selected for a case study. The simplified HD reactor was developed using as many locally available materials as possible and installed after a nitrification system, in order to eliminate the NO3 − -N generated during the nitrification process. This research was designed to support future efforts for deeper insight into the simplified HD reactor, which could potentially have important implications for the application of this technology in developing countries.

Description of the Study Area
The Kathmandu Valley is situated between the latitudes 27°32'13" and 27°49´10" N, and the longitudes 85°11´31" and 85°31´38" E. The study area, Jwagal, is located in the middle of the Kathmandu Valley ( Figure 1). In Jwagal, Kathmandu Upatyaka Khanepani Limited (KUKL) operates a water treatment unit to extract deep groundwater for intermittent distribution. The extracted groundwater is treated by aeration, coagulation and flocculation, sedimentation, sand filtration, and chlorination, as shown in Figure 2, before distribution to households.   Table 1 summarizes the groundwater quality of Jwagal, with data partially adapted from previous reports [11,37]. As can be seen in Figure 1b, the groundwater is turbid. The groundwater is heavily contaminated by NH4 + -N at concentrations ranging from 41.2 to information is available on the application of HD for raw groundwater treatment and the behavior of HD systems under on-site conditions. Therefore, the objective of this research is to develop a simplified, user-friendly HD reactor for people in the Kathmandu Valley, Nepal, and use it for raw groundwater treatment to evaluate its effectiveness, understand its behavior under on-site conditions, and fill the gap in knowledge between laboratory and practical applications. In this research, groundwater from the Jwagal area located in the Kathmandu Valley, Nepal, where the groundwater is highly contaminated by NH4 + -N, at levels up to 60 mg-N/L [11], was selected for a case study. The simplified HD reactor was developed using as many locally available materials as possible and installed after a nitrification system, in order to eliminate the NO3 − -N generated during the nitrification process. This research was designed to support future efforts for deeper insight into the simplified HD reactor, which could potentially have important implications for the application of this technology in developing countries.

Description of the Study Area
The Kathmandu Valley is situated between the latitudes 27°32'13" and 27°49´10" N, and the longitudes 85°11´31" and 85°31´38" E. The study area, Jwagal, is located in the middle of the Kathmandu Valley ( Figure 1). In Jwagal, Kathmandu Upatyaka Khanepani Limited (KUKL) operates a water treatment unit to extract deep groundwater for intermittent distribution. The extracted groundwater is treated by aeration, coagulation and flocculation, sedimentation, sand filtration, and chlorination, as shown in Figure 2, before distribution to households.   Table 1 summarizes the groundwater quality of Jwagal, with data partially adapted from previous reports [11,37]. As can be seen in Figure 1b, the groundwater is turbid. The groundwater is heavily contaminated by NH4 + -N at concentrations ranging from 41.2 to  Table 1 summarizes the groundwater quality of Jwagal, with data partially adapted from previous reports [11,37]. As can be seen in Figure 1b, the groundwater is turbid. The groundwater is heavily contaminated by NH 4 + -N at concentrations ranging from 41.2 to 57.3 mg-N/L, which exceeds the drinking water standard (1.2 mg-N/L) set by the World Health Organization (WHO) [16], and which cannot be removed by the current treatment process [9]. Thus, a nitrification system described by the Japan International Cooperation Agency (JICA) in 2019 [38] was supplementarily installed after the sedimentation system to remove NH 4 + -N from the groundwater.  Figure 3 shows a schematic of the on-site HD reactors. To ensure the sustainability of the water treatment unit and its compatibility with local conditions, reactors were made using as many locally available materials as possible. A cylindrical plastic water jar that is commonly used by households in Nepal [5,39] was selected for two attached growth reactors with a working volume of approximately 20 L. Polyolefin sponges (1 cm × 1 cm × 1 cm; Sekisui Aqua Systems Co., Ltd., Japan) were selected as the durable carrier material for better bacterial attachment and long-term applicability, and 1000 pieces were introduced into the inside of each reactor. The seed sludge for the on-site HD reactor was obtained from an HD reactor in operation [38,40]. A fiber carrier with 1.5 ± 0.4 g of volatile suspended solid (VSS) was used for acclimatizing the reactor. The two reactors were continuously operated in parallel from May 2017 to November 2017 with H 2 (hereafter D1) and without H 2 (hereafter D2) gas supply. The H 2 flow rate, flow rate of the influent, and hydraulic retention time (HRT) were maintained at 120 mL/min, 28 mL/min, and 12 h, respectively, during the operation. H 2 gas was supplied from a water electrolytic H 2 generator (HG-26; GL Science, Tokyo, Japan) through a commercially available aeration diffuser designed for aquariums (WP-1680, Sobo, China). Two reactors were installed after a nitrification system that treated the pretreated groundwater. The nitrification system comprises a dropping nitrification system, where the groundwater drips down from the top to the bottom by gravity. Nitrifying bacteria on the hanging materials consume oxygen in the air to convert NH 4 + -N to NO 3 − -N [20,40]. The nitrified groundwater was then supplied to the bottom of the HD reactors and passed through the carriers towards the outlet. The reactors were installed inside a room (dark) to prevent the algal growth. D1 and D2 were continuously operated for 157 and 113 d, respectively, without any internal cleaning. Samples of the influent and the effluents of D1 and D2 were taken randomly (at least once in every 10 days) to monitor the nitrogen concentrations, pH, dissolved oxygen (DO), water temperature, and turbidity during the operation period. In total, 21 samples from D1 and 18 samples from D2 were collected.

Batch Test for the Determination of NO3 − −N Removal and Denitrification Rate
Batch tests were conducted immediately before the start of the long-term operation and on Day 112 to determine the NO3 − -N and denitrification (NO3 − -N and NO2 − -N) rates in the same manner as previously described [21]. The rate was obtained from linear regressions based on the results of the test. The H2 flow rate was maintained at 120 mL/min during the batch test. The samples were collected from the reactor at regular intervals.

Analytical Methods
Collected samples were immediately filtered using a 0.45 μm pore-size membrane filter and stored in a freezer (−18 °C) until water quality analysis was performed. NO3 − -N, NO2 − -N, NH4 + -N, and bicarbonate (HCO3 − ) concentrations were analyzed by ultraviolet spectrophotometric screening, colorimetric, colorimetric-phenate, and titration methods, respectively, in accordance with standard methods [41]. The pH, DO, water temperature, and turbidity were measured using a pH meter (Horiba-B712, Kyoto, Japan), a portable DO meter (Multi 3410; WTW, Weilheim, Germany), a digital thermometer (WT-6, China), and a digital turbidity meter (TU-2016, Sato Shouji Inc., Saitama, Japan), respectively. The average temperature data of Kathmandu from May 2017 to November 2017 were obtained from the Historical Weather and Climate Data [42].

Performance of the Simplified HD Reactor
The nitrogen loading rate (NLR), nitrogen removal rate (NRR), and nitrogen removal efficiency were calculated based on Equations (1)-(3).

Batch Test for the Determination of NO 3 − −N Removal and Denitrification Rate
Batch tests were conducted immediately before the start of the long-term operation and on Day 112 to determine the NO 3 − -N and denitrification (NO 3 − -N and NO 2 − -N) rates in the same manner as previously described [21]. The rate was obtained from linear regressions based on the results of the test. The H 2 flow rate was maintained at 120 mL/min during the batch test. The samples were collected from the reactor at regular intervals.

Analytical Methods
Collected samples were immediately filtered using a 0.45 µm pore-size membrane filter and stored in a freezer (−18 • C) until water quality analysis was performed. NO 3 − -N, NO 2 − -N, NH 4 + -N, and bicarbonate (HCO 3 − ) concentrations were analyzed by ultraviolet spectrophotometric screening, colorimetric, colorimetric-phenate, and titration methods, respectively, in accordance with standard methods [41]. The pH, DO, water temperature, and turbidity were measured using a pH meter (Horiba-B712, Kyoto, Japan), a portable DO meter (Multi 3410; WTW, Weilheim, Germany), a digital thermometer (WT-6, China), and a digital turbidity meter (TU-2016, Sato Shouji Inc., Saitama, Japan), respectively. The average temperature data of Kathmandu from May 2017 to November 2017 were obtained from the Historical Weather and Climate Data [42].

Performance of the Simplified HD Reactor
The nitrogen loading rate (NLR), nitrogen removal rate (NRR), and nitrogen removal efficiency were calculated based on Equations (1)-(3).
Nitrogen removal efficiency (%) where In this study, NH 4 + -N concentration was not considered for NLR, NRR, or nitrogen removal efficiency, as HD is not meant to remove NH 4 + -N, and the difference in NH 4 + -N concentrations between the influent and effluent were not significant. Furthermore, the NH 4 + -N observed in the influent is attributed to the degraded performance of the nitrification system (NH 4 + -N concentrations should be less than 1.2 mg-N/L for drinking water).

Statistical Analysis
Data were first tested for normality using the Shapiro-Wilks test, and it was found that the data did not meet the normality assumption. Thus, to compare statistical differences among the three groups (influent, D1 effluent, D2 effluent) the non-parametric Kruskal-Wallis test and Dann-Bonferroni test were used. In all data, a p-value of less than 0.05 was considered statistically significant. The data were processed using the statistical analysis software package SPSS v.22 (IBM Corp., Armonk, NY, USA).  (Figure 4a). This demonstrates the rapid start-up ability of the HD and its stable performance even with the pretreated groundwater. The NO 3 − -N threshold for drinking water, 11.3 mg-N/L [16], was met for the entire test period. D2, however, showed a slight increase in NO 3 − -N concentration and a decrease in NH 4 + -N. The average NO 3 − -N concentration of the D1 effluent was significantly lower (p < 0.001) than that of the influent and D2 effluent, which clearly shows the effectiveness of the HD process for NO 3 − -N removal from raw groundwater. The NO 2 − -N concentration should be less than 0.9 mg-N/L for drinking water [16]. Although NO 2 − -N concentrations in the D1 effluent were found to be close to 0 mg-N/L (Figure 4b), the average was 2.1 ± 2.9 mg-N/L and the NO 2 − -N concentrations exceeded the limit in 11 out of the 21 samples taken. To avoid undesirable NO 2 − -N accumulation in the system and enhance the complete denitrification, the abundant addition of HCO 3 − (more than 3000 mg/L) could be a potential option [25].

Variations in Nitrogen Concentrations
The fluctuation of NH 4 + -N concentrations in the influent is attributed to the performance of the nitrification system (Figure 4c), which should produce concentrations lower than 1.2 mg-N/L for drinking water in practical application. The simplified HD reactor did not significantly affect the changes in NH 4 + -N concentrations, which is consistent with previous research [43,44].
The nitrogen removal efficiencies of D1 gradually increased throughout the experiment and reached 100% on Day 61, with an average of 80.9 ± 16.1% (Figure 4d), whereas nitrogen removal was not found in D2. Additionally, NRR varied from 18.3 to 73.7 g-N/(m 3 ·d), while NLR fluctuated from 23.8 to 92.3 g-N/(m 3 ·d). Furthermore, the batch experiments revealed that the NO 3 − -N removal and denitrification rate drastically increased compared to before the operation and Day 112 ( Table 2). These results confirmed that the simplified HD reactor has rapid start-up ability, stability, and robustness for processing pretreated groundwater. Water 2021, 13, x FOR PEER REVIEW 7 of 18   Figure 5a shows the variation of the average ambient temperature and the water temperature inside the reactors. The ambient temperature varied in the range of 14.4-26.5 °C, while the water temperature ranged from 22.5 to 28.1 °C. It is known that denitrification can occur over a wide range of temperatures, from 2 to 50 °C [45], and that HD occurs at temperatures between 15 and 50 °C [46]. Although a higher nitrate removal rate was obtained at higher temperatures [46], the HD efficiencies were maintained at a certain level (>70%) with a water temperature range of 15-30 °C [31]. In the present study, the water temperature inside the reactor stayed within this range for HD performance without temperature control.   Figure 5a shows the variation of the average ambient temperature and the water temperature inside the reactors. The ambient temperature varied in the range of 14.4-26.5 • C, while the water temperature ranged from 22.5 to 28.1 • C. It is known that denitrification can occur over a wide range of temperatures, from 2 to 50 • C [45], and that HD occurs at temperatures between 15 and 50 • C [46]. Although a higher nitrate removal rate was obtained at higher temperatures [46], the HD efficiencies were maintained at a certain level (>70%) with a water temperature range of 15-30 • C [31]. In the present study, the water temperature inside the reactor stayed within this range for HD performance without temperature control.   The pH during HD usually increases because the process produces OH − ions as a byproduct [25]. The average pH of the influent and the effluents of D1 and D2 were 7.9 ± 0.1, 9.0 ± 0.2, and 7.9 ± 0.2, respectively. The pH of the D1 effluent was significantly higher (p < 0.001) than that of the influent and D2 effluent (Figure 5b). These results indicate the occurrence of microbial activity in the D1 reactor.

Ambient Condition and Changes in Operational Parameters
The DO concentration in D1 was kept low, ranging from 0.05 to 0.87 (Figure 5c), with an average value of 0.3 ± 0.2 mg-O 2 /L, which could be the result of H 2 gas supply and denitrification. Meanwhile, the DO concentrations in the influent and D2 ranged from 3.2 to 8.2 and 0.15 to 5.40 mg-O 2 /L, respectively. The effluent of the nitrification system contains high DO as previously reported [20], which is the same as the influent of the simplified HD reactor. However, the HD system can be inhibited by high DO concentrations [47,48], and Singhopon et al. has suggested that the DO value should be between 0.5 and 0.8 mg-O 2 /L to improve NRR [48]. In the present study, supplying H 2 gas into the reactor was instrumental in maintaining the preferred range of DO concentrations for HD. Figure 5d depicts the variations in HCO 3 − concentration during the operation. A total of 0.171 moles of HCO 3 − was consumed to remove 1 mole of NO 3 − based on the stoichiometry of the HD [49]. The maximum NO 3 − -N concentration found in the influent during the operation was 39.5 mg-N/L; thus, approximately 30 mg/L of HCO 3 − would be required for the complete removal of NO 3 − -N. In previous research, inorganic carbon sources were added to HD systems to improve stability [50], but this increases their operational costs. The average HCO 3 − concentration in the influent was 404.5 ± 56.8 mg/L. This shows that the groundwater in Jwagal naturally contains enough bicarbonate for the HD to proceed without additional inorganic carbon input. Figure 5e profiles turbidity in the simplified HD reactor. Turbidity during the HD process has not been widely reported to date. The turbidity can influence not only the aesthetics or acceptability of drinking water but also disinfection treatment [51]; thus, it is important to know the impact of the simplified HD reactor on turbidity. In the case of household water treatment, the WHO suggests that turbidity should be at least less than 5 NTU, and ideally turbidity should be less than 1 NTU as regards aesthetics and disinfection efficiency [51]. In this study, turbidity was higher than 1 NTU, with averages of 2.0 ± 0.9, 2.2 ± 0.9, and 1.2 ± 0.7 NTU for the influent, D1 effluent, and D2 effluent, respectively. Thus, a post-treatment after the simplified HD reactor is recommended.

Comparison with Various HD Reactors
Most research on HD technologies developed for groundwater treatment has been conducted in laboratories using synthetic groundwater. To compare the reactor performance between synthetic and raw groundwater treatment, various studies on HD reactors are summarized in Table 3. In the table, experiments using different types of reactor, substrates, bacteria inoculums, temperatures, HRTs, H 2 availability, influent NO 3 − -N concentrations, carrier materials, reactor volumes, NLR, NRR, and nitrogen removal efficiencies are compared. The simplified HD reactor developed in this research exhibited nitrogen removal efficiencies as high as those of other reactors in Table 3 (~100%), and showed 73 g-N/(m 3 ·d) of NRR, which is higher than other techniques shown in Table 3, such as bio-ceramite reactors [31], heterotrophic denitrification coupled with electro-autotrophic denitrifying packed bed reactors [32], and HD with electrolytic reactors [36]. Although hollow fiber reactors [26], submerged membrane reactors [28], and membrane biofilm reactors [30] possessed higher NRR (110-770 g-N/(m 3 ·d)) than attached growth reactors, clogging often occurs using these techniques, necessitating frequent cleaning and cost for the replacement of the fiber or membrane [45]. Fluidized bed reactors can also achieve a high NRR (2160 g-N/(m 3 ·d)) [29]; however, the system itself is far more complicated and hence is difficult to control [45]. The unsaturated flow pressurized reactor also exhibited a high NRR (2100 g-N/(m 3 ·d)) and has simplicity, a small size, and is safe, although frequent cleaning of these reactors might be needed [33].         The NRR of the simplified HD reactor (73 g-N/(m 3 ·d)) was similar to that of suspended or attached growth reactors (e.g., 69.1 [23], 77.2 [24], and 78.4 g-N/(m 3 ·d) [34]). Higher NRRs of 167 and 107 g-N/(m 3 ·d) were obtained using attached growth reactors [19] for synthetic and raw groundwater, respectively. However, the supplied H 2 gas for these two reactors was 23 mL/(min·L), whereas 6 mL/(min·L) was supplied for the simplified HD reactor developed in this study.
To ensure sustainability and the compatibility of the water treatment unit with local conditions, the simplified HD reactor was made out of an inexpensive jar that is available all over Nepal, resulting in a low initial cost for the installation of the reactor. It should be noted that the reactor was continuously operated for 157 days without temperature control, cleaning, or complex maintenance. This demonstrates the ease and simplicity of the system, which facilitates local engagement with the technology. Therefore, the simplified HD reactor is a more user-friendly option for NO 3 − -N removal from groundwater in the Kathmandu Valley, Nepal.

Application and Implementation of the Simplified HD Reactor
Groundwater in the Kathmandu Valley is contaminated by NH 4 + -N and NO 3 − -N, as previously reported [11][12][13][14][15]. NH 4 + -N removal can be conducted by combining the previously developed nitrification system [18][19][20] and the simplified HD reactor developed in this study (Figure 6a), whereas NO 3 − -N removal can be performed using the simplified HD reactor alone (Figure 6b). However, the simplified HD reactor sporadically emitted an odor during operation, which might affect the acceptability of the treated water. Therefore, filtration with activated carbon, followed by disinfection, is recommended for installation after the simplified HD reactor. The filtration with activated carbon is to remove the odor, turbidity, and dissolved organic compounds (if any) [40]. The activated carbon is recommended to be replaced on a yearly basis [40]. Although chlorination is more common in Nepal for water disinfection, ultra-violet (UV) disinfection could be a better alternative for household scale.

Application and Implementation of the Simplified HD Reactor
Groundwater in the Kathmandu Valley is contaminated by NH4 + -N and NO3 − -N, as previously reported [11][12][13][14][15]. NH4 + -N removal can be conducted by combining the previously developed nitrification system [18][19][20] and the simplified HD reactor developed in this study (Figure 6a), whereas NO3 − -N removal can be performed using the simplified HD reactor alone (Figure 6b). However, the simplified HD reactor sporadically emitted an odor during operation, which might affect the acceptability of the treated water. Therefore, filtration with activated carbon, followed by disinfection, is recommended for installation after the simplified HD reactor. The filtration with activated carbon is to remove the odor, turbidity, and dissolved organic compounds (if any) [40]. The activated carbon is recommended to be replaced on a yearly basis [40]. Although chlorination is more common in Nepal for water disinfection, ultra-violet (UV) disinfection could be a better alternative for household scale. According to the results obtained in this study, the simplified HD reactor can currently produce 40 L of drinking water per day, operating with a working volume of 20 L and an HRT of 12 h. Basic water requirements comprise four basic human needs: drinking water for survival, water for human hygiene, water for sanitation services, and modest household needs for preparing food [52], and these needs are estimated to require 50 L per capita per day (LPCD) [52]. Although the current simplified HD reactor is not capable of meeting all of these basic water requirements, it could provide the drinking water and water for cooking for a household. The WHO estimates that daily drinking water con- According to the results obtained in this study, the simplified HD reactor can currently produce 40 L of drinking water per day, operating with a working volume of 20 L and an HRT of 12 h. Basic water requirements comprise four basic human needs: drinking water for survival, water for human hygiene, water for sanitation services, and modest household needs for preparing food [52], and these needs are estimated to require 50 L per capita per day (LPCD) [52]. Although the current simplified HD reactor is not capable of meeting all of these basic water requirements, it could provide the drinking water and water for cooking for a household. The WHO estimates that daily drinking water consumption per capita is 2 L for adults, and it is reported that the average size of a family in Nepal is 4.6 persons [53]. Thus, it can be assumed that a household requires approximately 9-10 L of drinking water per day, which is consistent with the value of 9.4 L from previously reported questionnaire survey results [8]. Furthermore, water consumption for cooking was reported to be 3-6.5 LPCD, depending on household income [54], which is equivalent to 12-33 L for a household per day. Therefore, the simplified HD reactor can cover the water requirements for drinking and cooking purposes at the household level.
In the present study, H 2 gas was supplied from a water electrolytic H 2 generator, resulting in high installation costs and frequent maintenance, such as the need to add water and replace the generator's drying agent [40]. Frequent power cuts in Nepal [55], which interrupt the operation of H 2 generators, should also be considered. In the long run, the generator should be substituted with easier and cheaper alternatives, and H 2 should be efficiently used to reduce maintenance and cost. Furthermore, the sponge materials used in this study were not locally available; thus, a simplified HD reactor with locally available materials should be tested to investigate treatment capacities using possible substitutes for the current sponge material.

Conclusions
A case study was conducted to develop a simplified HD reactor using as many locally available materials as possible, and the reactor was installed in the Kathmandu Valley, Nepal to evaluate its effectiveness, understand its behavior under on-site conditions, and fill the gap in knowledge between laboratory and practical applications. NO 3 − -N removal by the reactor was found to be effective, and the reactor showed rapid start-up ability, stability, and robustness against pretreated groundwater. The reactor had an average nitrogen removal efficiency of 80.9 ± 16.1%, while NLR and NRR varied from 23.8 to 92.3 g-N/(m 3 ·d) and from 18.3 to 73.7 g−N/(m 3 ·d), respectively. The simplified HD reactor can be operated under on-site conditions without temperature control, supplementary DO concentration control, an additional inorganic carbon source, or internal cleaning. Compared to the results of previous research, the simplified HD reactor was considered a more user-friendly option for people in the Kathmandu Valley, Nepal. Additionally, the reactor is recommended for groundwater treatment at the household level, as it can currently produce 40 L/d, fulfilling the daily requirements for drinking and cooking water in a household with 4-5 people. The findings of this research would be helpful for practical applications of HD in developing countries.