The Seagrass Holobiont: What We Know and What We Still Need to Disclose for Its Possible Use as an Ecological Indicator

Microbes and seagrass establish symbiotic relationships constituting a functional unit called the holobiont that reacts as a whole to environmental changes. Recent studies have shown that the seagrass microbial associated community varies according to host species, environmental conditions and the host’s health status, suggesting that the microbial communities respond rapidly to environmental disturbances and changes. These changes, dynamics of which are still far from being clear, could represent a sensitive monitoring tool and ecological indicator to detect early stages of seagrass stress. In this review, the state of art on seagrass holobiont is discussed in this perspective, with the aim of disentangling the influence of different factors in shaping it. As an example, we expand on the widely studied Halophila stipulacea’s associated microbial community, highlighting the changing and the constant components of the associated microbes, in different environmental conditions. These studies represent a pivotal contribution to understanding the holobiont’s dynamics and variability pattern, and to the potential development of ecological/ecotoxicological indices. The influences of the host’s physiological and environmental status in changing the seagrass holobiont, alongside the bioinformatic tools for data analysis, are key topics that need to be deepened, in order to use the seagrass-microbial interactions as a source of ecological information.


Introduction
Seagrasses are marine angiosperms that returned from the land to the marine environment 60-90 MYA in multiple events, resulting in a paraphyletic group composed of four families, three of which include only marine species [1]. Seagrasses are widely distributed in coastal waters all over the world (except Antarctica), from very shallow to 90 m depth [2,3]. Considered as ecosystem engineers (sensu Wright and Jones [4]), seagrasses form extensive meadows, which are the foundation of complex systems. These meadows provide key ecosystem services [3], i.e., natural processes or components that benefit human needs [5]. The three-dimensional structure of seagrass meadows provides critical habitats for many organisms and also serves as a nursery ground for juvenile stages of many species, including economically important species of finfish and shellfish [6][7][8]. In addition, seagrasses stabilize soft sediments and stock large quantities of CO 2 as biomass and dead organic matter (blue carbon), contributing to the mitigation of anthropogenic emissions [3,9,10].
Despite the ecological and economic importance of seagrass beds, an increasing number of reports have documented the ongoing loss of seagrass biomass in several countries, with a global decline rates estimated at 2-5% per year [11]. Most of these losses have been associated with human-driven pressures, including increased nutrient and sediment runoff, pollution, hydrological alterations, invasive species, commercial fishing or aquaculture practices. Not only do these pressures continue to threaten seagrass meadows, but they have also already shown to have caused large seagrass losses and regression worldwide, affecting entire ecosystems with a bottom up effect [12][13][14]. Despite the recent slowing down of these trends, mainly of fast-growing species [15], the overall worldwide trend is still the decline or deterioration. These ongoing declines are particularly high for the more slow-growing seagrasses (e.g., Posidonia oceanica; the foundation of one of the most valuable Mediterranean ecosystems), where reductions and a lack of recovery cause severe losses of some of the ecosystem services provided, since their ecological functions and services cannot be replaced by the fast growing seagrass species [5,16].
To mitigate seagrass losses, it is essential to detect the environmental changes and seagrass stressors, prior to the decline (sometimes irreversibly) in local meadows occurs. The current challenge, thus, is the development and the combination of sensitive and measurable descriptors of seagrass stress, able to assess seagrass ecological state and its alterations. This will help to prevent the decline and enhance our understanding of seagrass global processes and threats, further supporting the development of effective monitoring and integrated management programs [17][18][19][20].
To date, seagrass descriptors have focused on different biological organization levels from the population to the individual levels, such as shoot density, alongside biochemical and genetics descriptors [19,20]. The time of response of such indicators to stressors generally increases with the structural complexity, while their specificity decreases [18]. With most the descriptors used these days in seagrass monitoring programs being based on the slow responding population level (e.g., species composition, percent of cover, density, etc.), there is a growing interest in shortening the time of response by identifying early warning indicators. Recent studies [21][22][23][24][25][26][27][28][29][30][31][32] have evaluated the pivotal role of the microbial community associated with seagrass in their physiology and ecology, assessing their symbiotic relationships and the variability of microbes according to the environmental/host conditions, arguing that the host associated microbes could be a sensitive monitoring tool and ecological indicator. In fact, as microbial communities respond rapidly to environmental disturbance, monitoring their composition could represent an early indicator of environmental stress [21,22,24,25,29,31,32]. Despite the encouraging results of the abovementioned studies, the comprehension of the seagrass associated microbial community variation according to the host conditions is still far from being clear.
The seagrass-microbes associations are the result of a selective process involving both the seagrass microenvironment availability-depending on the host ecological/physiological conditions in response to the environment, and the metabolic capabilities of the microbes. The intimate relationship between the host and its associated microorganisms has led to considering them as a complex single super-organism that jointly responds to environmental changes as a functional complex unit, called the holobiont [33]. This perspective significantly changes the way of thinking of a living-organism and may provide important insights into the organism condition and, consequently, into the potential use of associated microbes as a source of ecological information.
While several seagrass-microbe interactions, mainly studied as belowground functional processes, have been identified (see Section 1.2); a unified point of view about the factors implicated in the settling, composition and spatial-temporal variation of the associated microbial communities, is still missing. One of the main questions that need addressing is whether seagrass species-specificity can be hypothesized. In other words, does each seagrass species harbors its own specific taxonomic and/or functional microbiota across different sites, or is the seagrass microbiota shaped by the environmental conditions and follow a common pattern in different seagrass species growing in the same habitat. This is a pivotal point that would give insights into both the seagrass' capability to select their hosts and the ecological meaning of variations in the microbial component of the seagrass holobiont: elucidating the key host-microbe interactions may provide guidance for seagrass managing and/or restoration [34].
Often, different studies found heterogeneous results on the epiphytic microbial taxonomic composition; in some cases species-specific differences were found [35][36][37], while in others comparable microbial communities were found associated with different seagrass species from the same site [27,38,39]. The species-specificity of the microbial profile has been related to specific biochemical properties, while the comparable microbial communities were mainly attributed to the local environmental conditions.
The aim of this work is to review all the information available to date that can support the potential use of associated microbes as early warning indicators of seagrasses and marine environment ecological status. The following paragraphs summarize the stateof-the-art on seagrass holobiont, focusing on the aspects that need to be deepened in order to develop an integrated seagrass microbiome-based index. These include, from our point of view, the associated microbes' variability according to the host species/host physiology/environmental conditions and the bioinformatics aspects, which is crucial to have reliable and homogeneous data.

The Holobiont Concept
Host-microbes interactions play crucial roles in biological and ecological functions, thus, organisms are better considered as a network of interactions between the host and all the associated microorganisms (bacteria, fungi and viruses), with which the host establishes transient or lasting complex relationships [40][41][42]. The host and the entirety of microorganisms living in/on its tissues represent a complex functional unit, the holobiont [33].
Lynn Margulis [43][44][45] was the first to emphasize the role of the symbiosis, and in particular the endosymbiosis, as an evolutionary trajectory. Her studies are considered the starting point of this research field, despite similar ideas having already circulated years before, due to the less known German scientist Meyer-Abich [46]. Working mostly on microbial communities of corals, Zilber-Rosenberg and Rosenberg implemented this concept, considering the holobiont as an additional organismal level on which natural selection may operate [47,48], and defining the hologenome as the integration of the host genome with the gene pool of the associated microorganisms [47,[49][50][51]. In this review, we will focus on the interactions between the host (seagrass) and the bacterial partners but, of course, also other microorganisms may contribute to the holobiont.
The role of the host-microbe interactions, as an evolutionary driving force, is still unclear [48,[50][51][52]; however, the importance of considering such interactions in the host physiology and ecology is indubitable [53,54]. In fact, the holobiont changes according to the environmental changes and maintains the host-microbes homeostasis, and its disruption may lead to pathologic conditions [55][56][57][58]. For instance, the rapid changes in the microbiota, in terms of changing community structure and composition, mutations or horizontal gene transfer, facilitate the holobiont adaptation to the continuous and unpredictable changing environmental conditions [24,52,54].
A remarkable difference among the microbial communities associated with the aboveand belowground seagrass plant compartments has been found [24,25,31,37,39]. This is not surprising, as the two parts are positioned in very different environments in terms of light, oxygen, redox gradient and carbon availability. Furthermore, the seagrass plants themselves establish diverse chemical microenvironments: leaves release organic carbon, producing organic carbon enriched habitats [78]; roots supply their surrounding sediment with oxygen, creating aerobic microzones in the anoxic sediment and redox gradients that lead to phosphorus and iron mobilization into the rhizosphere [26,29,84]. The aboveground compartment is colonized by a large variety of generalist, aerobic organo-heterotrophic taxa, able to degrade common plants' polymers, waste compounds and biofilm [25,78]. The belowground compartment, due to the partial presence of oxygen, harbors both anaerobic and aerobic microorganisms, such as chemolithotrophic, sulfur-oxidizing and nitrogenfixing microorganisms [25][26][27][28]78]. Due to the radial loss of oxygen and to the release of exudates [26,89,90], the belowground compartment stimulates a selective microbial growth and in turn, receives benefits from bacterial metabolism [91,92]. Few studies have analyzed the rhizomes as a separate plant part [37,93] finding that it harbors unique microbial communities, although some microbial groups are shared with roots and sediment. As a final point, Hurtado-McCormick et al. [93] hypothesized that the local physiological activities of the seagrass may produce a further differentiation of the colonized surfaces in microhabitats. They did not find significant differences among leaves' microhabitats (i.e., upper or lower side of the leaf), although they highlighted clear differences among rhizome/roots colonizers, probably depending on local selective environmental conditions (mentioned above).
The genetic/metabolic versatility of microbes is one of the key points of the plant/ microbes association [27,94,95]. Microbe versatility contributes to the plant nutrient supply, acting in the sulfur and nitrogen cycles [85,91,92,96,97]; this represents a benefit for seagrasses and allows us to infer, which are the main metabolic patterns that are taking place around the roots of seagrasses. For instance, the nifH gene (marker of nitrogenase activity) that demonstrates the presence of nitrogen-fixing bacteria, found in the microbial community associated with roots of P. oceanica, was found to belong to sulfur-oxidizing or sulphate-reducing bacteria [92], suggesting that the nitrogen-fixing bacteria may play different roles, other than being an important source of nitrogen for seagrasses [95][96][97][98][99]. Similarly, a metagenomic study on the bacteria associated with Zostera marina's roots highlighted the presence of several genes involved in sulfur oxidation, nitrate reduction and carbon fixation [27,28].
Seagrass sediments contain organic matter due to plant debris, benthic or sink dead organisms that sunk to the bottom, and to root exudates [80]. In sediments, microbial mineralization can be either aerobic, in the thin layers of the upper sediment and around the seagrass' oxygen leaking young root tips, or anaerobic in deepest sediment layers, beyond the effect of the seagrasses' roots [26,99,100]. The anaerobic decomposition involves sulfate-reducing bacteria and leads to the accumulation of phytotoxic H 2 S into the sediment, a mechanism was suggested to be responsible for historical events of wide seagrasses die-off [29,85,101]. Seagrasses tolerate low concentration of H 2 S [85,101,102], and they overcome its toxicity by translocating the photosynthetically produced oxygen, from the leaves to the rhizosphere supporting local spontaneous H 2 S reoxidation [103,104]. This allows the thriving of sulphate-oxidizing bacteria [26,28,89], found to be less abundant in low-light conditions [29].
Diazotrophic bacteria, which enhance seagrass nitrogen availability [30,86,98,[105][106][107], have been found in both the above-and the belowground seagrass tissues and are more abundant in vegetated sediment than in the bare one [95,107]. For instance, cyanobacteria were found to be more abundant on seagrass leaves from oligotrophic environments [35,107], and Tarquinio et al. [30] demonstrated that leaves of the temperate seagrass Posidonia sinuosa with associated microbes, accumulate more nitrogen than those devoid of microorganisms. In this laboratory study, Tarquinio et al. [30] incubated seagrass leaves with amino acids labeled with stable isotope 15 N and found that the marked amino acids were assimilated by the associated microbes and by plants, but not by plants without microorganisms. This demonstrated the active role of seagrass associated microbiota both in supplying nitrogen to plants and in amino acid mineralization.
Nitrogen fixing bacteria play another important role in the seagrass holobiont, being also involved in the phytotoxic sulfate-reduction, a key-role in organic matter mineralization. The seagrass release of photosynthates translocated from roots to the rhizosphere sustains epibionts associated to their tissues or in the close surrounding environment [26,29,89,107], suggesting a mutualistic relationship between the seagrass host and its diazotrophic bacteria, and the role of these microorganisms in maintaining seagrass health [30,32].
Thus, the microenvironments of seagrass surface could be a selective substrate for microbial growth, although this hypothesis deserves further investigation. Research in this field will surely provide new insights into plant ecology and associated microbial community dynamics, an important step towards seagrass monitoring and conservation [32,34,79].
A last critical point for the holobiont comprehension is to understand the process of microbial colonization of the seagrass compartments. It must rely on the environmental microbial pool, as so far no other microbial sources have been detected [120]. This colonization process may follow different pathways: microbial communities associated with leaves generally mirror those present in the surrounding seawater column, while microbial communities associated with the rhizome/root usually strongly differs from the sediment microbial community [120].
A recent study by Kohn et al. [121] focused on the variation of microbial communities on the leaves of the same Posidonia oceanica plants over time: the authors showed that a macroscopically different biofilm is found in young and older leaves, with increased diversity in older leaves, but with very similar taxonomic compositions. The influence of age on root-associated microbial biofilm was investigated but no clear trends were found [22,122]. However, the colonization time is probably an important factor shaping the seagrass holobiont, and deserves further attention.
Looking at the ecosystem level, interactions among seagrasses and microorganisms can directly influence large-scale biogeochemical processes, including coastal carbon sequestration [123][124][125][126][127][128][129][130][131][132][133], the so-called blue carbon [124]. Seagrass ecosystems are significant carbon sinks, as both living plant biomass and recalcitrant dead organic matter, and the degradative activity of the sediment microbial communities control the amount of sequestered carbon [128,131]. Recent studies showed an increased mineralization rate of organic carbon in sediments as a response to eutrophication and warming seawater temperatures. In fact, both eutrophication and warming seawater temperatures may stimulate microbial metabolism and speed-up the mineralization processes, enhancing CO 2 release within the water column [126,129,131,132,134]. Contrasting results were found by investigating whether the nutrient input stimulates the mineralization process in vegetated sediments [129,131]. These results suggest the co-occurrence of other factors that are able to drive the microbial response to increased nutrient availability [131]. On the contrary, rising temperatures seem to have a significant effect on the aerobic mineralization of organic carbon, but a negligible effect on both the anaerobic mineralization and the recalcitrant seagrass dead tissue mineralization, as debris of rhizomes and roots [126,[131][132][133][134]. Thus, the aerobic mineralization of exposed buried carbon (as in the case of sediment resuspension by trawling) may reduce the carbon sink capacity of seagrass dead organic matter, through increased microbial abundance and speed-up of the mineralization process [133,134]. Hence, seagrass-microbes interactions may clearly affect ecosystem processes and these interactions, usually evaluated at small scales, have to be upscaled and evaluated even at a wide seascape. To this end, the study of seagrass holobiont variations in the field and/or under controlled laboratory conditions is pivotal to point out which factors shape and regulate these interactions. Such studies could contribute to seagrass monitoring and conservation efforts.

A Case Study: The Halophila stipulacea Holobiont
Halophila stipulacea is a small fast growing tropical seagrass [3], native to the Red Sea, Persian Gulf and the Indian Ocean ( Figure 1a) [135]. Following the opening of the Suez Canal, it settled in the Mediterranean Sea 150 years ago as a Lessepsian migrant [105,[135][136][137][138], where it remained in insulated, small populations across the eastern part of the basin ( Figure 1b). Surprisingly, in 2002, Halophila stipulacea was reported in the Caribbean Sea, where within less than two decades it spread to most of the Caribbean Island nations and has even reached the South American continent (Figure 1c) [139][140][141][142][143][144]. Unlike its limited invasion of Mediterranean, in the Caribbean H. stipulacea creates large, continuous populations often completely displacing local Caribbean seagrass species [135,[139][140][141][142][143][144]. Realizing that the Caribbean Halophila situation might repeat itself in the Mediterranean Sea in the coming future, scientists have already begun to sound their alarms. Indeed, the potential threat to local biodiversity posed by H. stipulacea was raised by including H. stipulacea in the "100 Worst Invasive Alien Species in the Mediterranean" [145]. With the recent doubling of the Suez Canal (July 2015) and the warming of Mediterranean Sea-one of the basins most rapidly changing under climate change [146]-there is great concern that the so far limited population of H. stipulacea may spread. The species, in fact, is already expanding, competing with the local Cymodocea nodosa [147]. The invasiveness of H. stipulacea is probably due to its capabilities to thrive under different ecological conditions including different salinity, light and water temperature [148][149][150][151], being able to easily adapt its morphophysiological parameters to changing environmental conditions [24,25,105,135,150,[152][153][154][155][156][157][158][159][160]. H. stipulacea is also able to modify its morphology: it shows increasing leaf size along depth gradients [25,[152][153][154]156,157,159,160] and small leaf size (width and length) under high light levels and/or high temperature and hydrodynamics [25,152,155]. The plant can also rapidly adjust its photosynthetic responses [24,25,135,148,159,160] and the synthesis of secondary metabolites, such as total phenols [24,25,153] according to environmental conditions. Even the epiphytic bacterial community associated with H. stipulacea varies across environmental conditions. This suggests a crucial involvement of the seagrass-associated microbiome in the host physiological functions of the host and a potential contribution to the adaptive and invasive capabilities of this seagrass species [24,25].
The microbial community associated with H. stipulacea has been studied, so far, only in its native area (Red Sea) [24,25,37,105,161,162]. Some "historical" studies were carried out with culture-dependent analyses [37,105]; these studies highlighted the differences of the bacterial community associated with different plant compartments, with higher density of heterotrophic microbes found on its leaves. Moreover the leaf's microbial community followed the seawater microbial community seasonal variation [37], and different nitrogen-fixing microbes on the leaves (light dependent diazotrophic bacteria that performed non-oxygenic photosynthesis), on the upper-rhizomes (Cyanobacteria) or on the roots (heterotrophic diazotrophs) [105]. This suggests that H. stipulacea associated microbes can fix nitrogen in different conditions (and plant compartments), thus supplying the plants with the nitrogen needed, a potential evolutionary advantage ascribable to the seagrass-microbial symbiosis.
Weidner et al. [161,162] identified on the leaves of H. stipulacea a diverse assemblage of bacteria mainly belonging to Alpha-and Gamma-Proteobacteria, by using a restriction fragment length analysis termed ARDRA (amplified rDNA restriction analysis).
More recent studies [24,25] applied the analyses of the 16S rDNA amplicons (obtained by 454-pyrosequencing or Illumina sequencing) to characterize the taxonomic composition of the microbial epiphytic communities associated with H. stipulacea alongside measurements of seagrass morphological and biochemical indexes (e.g., leaf morphometrics, pigments and phenols content), in different ecological conditions. In the Gulf of Aqaba, under different anthropogenic pressure (i.e., coastal urbanization, fish farming activities, commercial maritime facilities, etc.), depths, topography and hydrodynamics (adaptation at small spatial scale) H. stipulacea was found to modulate: (i) leaf morphology and photosynthetic pigment content according to light availability, depth and hydrodynamics; (ii) total phenol content according to the anthropogenic pressure (and to irradiance) and (iii) the microbial communities according to local environmental conditions and plant The invasiveness of H. stipulacea is probably due to its capabilities to thrive under different ecological conditions including different salinity, light and water temperature [148][149][150][151], being able to easily adapt its morphophysiological parameters to changing environmental conditions [24,25,105,135,150,[152][153][154][155][156][157][158][159][160]. H. stipulacea is also able to modify its morphology: it shows increasing leaf size along depth gradients [25,[152][153][154]156,157,159,160] and small leaf size (width and length) under high light levels and/or high temperature and hydrodynamics [25,152,155]. The plant can also rapidly adjust its photosynthetic responses [24,25,135,148,159,160] and the synthesis of secondary metabolites, such as total phenols [24,25,153] according to environmental conditions. Even the epiphytic bacterial community associated with H. stipulacea varies across environmental conditions. This suggests a crucial involvement of the seagrass-associated microbiome in the host physiological functions of the host and a potential contribution to the adaptive and invasive capabilities of this seagrass species [24,25].
The microbial community associated with H. stipulacea has been studied, so far, only in its native area (Red Sea) [24,25,37,105,161,162]. Some "historical" studies were carried out with culture-dependent analyses [37,105]; these studies highlighted the differences of the bacterial community associated with different plant compartments, with higher density of heterotrophic microbes found on its leaves. Moreover the leaf's microbial community followed the seawater microbial community seasonal variation [37], and different nitrogen-fixing microbes on the leaves (light dependent diazotrophic bacteria that performed non-oxygenic photosynthesis), on the upper-rhizomes (Cyanobacteria) or on the roots (heterotrophic diazotrophs) [105]. This suggests that H. stipulacea associated microbes can fix nitrogen in different conditions (and plant compartments), thus supplying the plants with the nitrogen needed, a potential evolutionary advantage ascribable to the seagrass-microbial symbiosis.
Weidner et al. [161,162] identified on the leaves of H. stipulacea a diverse assemblage of bacteria mainly belonging to Alpha-and Gamma-Proteobacteria, by using a restriction fragment length analysis termed ARDRA (amplified rDNA restriction analysis).
More recent studies [24,25] applied the analyses of the 16S rDNA amplicons (obtained by 454-pyrosequencing or Illumina sequencing) to characterize the taxonomic composition of the microbial epiphytic communities associated with H. stipulacea alongside measurements of seagrass morphological and biochemical indexes (e.g., leaf morphometrics, pigments and phenols content), in different ecological conditions. In the Gulf of Aqaba, under different anthropogenic pressure (i.e., coastal urbanization, fish farming activities, commercial maritime facilities, etc.), depths, topography and hydrodynamics (adaptation at small spatial scale) H. stipulacea was found to modulate: (i) leaf morphology and photosynthetic pigment content according to light availability, depth and hydrodynamics; (ii) total phenol content according to the anthropogenic pressure (and to irradiance) and (iii) the microbial communities according to local environmental conditions and plant compartments. A high incidence of Gammaproteobacteria and Bacteroidetes was found in light-limiting conditions, while Cyanobacteria and Rhodobacteraceae thrived in conditions of high light availability and hydrodynamics. The Alphaproteobacteria were dominant on leaves while Alfa-, Gamma-and Delta-Proteobacteria were equally distributed on roots. The less represented microbial groups were specific to each site, suggesting that the microbial community associated with H. stipulacea was made up of a conserved main core (i.e., a "core microbiome" shared across all sites) and a highly variable small component that may help plants to cope locally with environmental pressure (i.e., a local environmental fingerprint). The Alphaproteobacteria class, in particular the Rhodobacteraceae family, may be abundant on the leaves due to their abundance in the water column and in oligotrophic coastal waters [163,164] and its capability to cope with variations of salinity, oxygen saturation, pH, nitrate concentration and temperature [165,166].
The studies reviewed above found some common points of the H. stipulacea holobiont in the Red Sea. All of them highlighted a marked difference between the microbial communities associated with above and the belowground plant parts, as assessed in other seagrass microbiota studies. Moreover, other studies [24,25,161,162] found common microbial groups associated with this seagrass. Among these are mentioned the Alphaproteobacteria class and in particular the Rhodobacteraceae family, Gamma-and Delta-Proteobacteria and low abundance of Actinobacteria; these data support the existence of a local microbial core, where microbial groups could play crucial roles in H. stipulacea functions in that environment [25,167]. For example, it has already discussed the potential role of Actinobacteria group in providing antimicrobial compounds and the capability of members of the Pseudomonas genus to produces antifouling compounds and the presence of nitrogen fixing bacteria, like Planctomycetes [105], or denitrifying microbes as Pseudomonadaceae [168].
Studies by Mejia et al. [24] and Rotini et al. [25], relating the microbial analyses to different environmental conditions and to seagrass ecophysiological analyses, provided insights into the holobiont functionality. For instance, the co-occurrence increase of leaf phenol content and leaf microbial abundance, suggests a role of phenols in mediating the seagrass-microbial interaction or the induction of phenols production as a chemical defense against microbes. H. stipulacea, in fact, has high content of flavones [169][170][171][172] and phenols [24,25]. These classes of compounds are secondary metabolites known to be involved in plant defense and in regulating microbial interactions [20,171,173].
Collectively, the discussed studies build up a useful baseline of the symbiotic relationships between microbes and H. stipulacea, offering new tools to understand the plasticity and the adaptive capabilities of this species. On the other hand, these studies suggest that H. stipulacea can be a good model organism to investigate in depth the seagrass holobiont structure and functionality.
Further studies are needed to clarify the seagrass-microbial interaction and, in particular, to clarify the variation of the seagrass holobiont related to the seagrass ecophysiology and environmental conditions. Studies in this direction are in progress on the invasive H. stipulacea, in both the Mediterranean and the Caribbean Seas, in order to evaluate the microbial role in its invasiveness potential. Hopefully, this research will place the seagrass holobiont into the wider frame of the ecosystem processes.

Plant Species
Regarding the role of the host seagrass species, previous studies have resulted in contrasting evidence. On the one hand, studies have shown that within the same geographical site, microbial communities on different seagrass species were similar [27,38,39]. On the other hand, studies have also shown that there were significant differences in terms of microbial community composition between different seagrass species growing side by side [29,[35][36][37]174,175]. In parallel, differences in the composition of microbial communities were also found in the same seagrass host species growing at different sites [24,25,120,[176][177][178]. These contrasting results may depend on plant structure (i.e., plant habit; [36,175]), composition of meadows (i.e., mixed or monospecific stands; [27,35]), sampling time (i.e., season, daytime; [31,36]) or environmental/host conditions (i.e., light, seawater acidification; [23,29]), all major factors influencing the microbiota composition.
The species-specificity usually does not result from the overall community profile but from the less abundant components [35,174,175] that could highlight specific functional associations [39].
The prevalence of bacterial taxa associated with different species in the same site [27,38,175,179] or with the same seagrass species growing in different sites/conditions [24,25,93,120] is often defined as the "microbial core community". Probably, instead of a generally important seagrass-microbial core, a local core can be identified [25,31,167], playing environmentally specific functions [93]. The identification of the core and the changing components of the microbial partners is a key-point in understanding the dynamics of the holobiont.
As a general rule, the aboveground associated microbial communities are similar to those found in the surrounding seawater [120,176], and were found to be more variable compared with the microbial communities growing on the belowground plant compartments [24,25,27,31], highlighting the importance of environmental fluctuations (more probable in seawater than in sediment) in eroding the stability of bacterial community assembly [25]. The high variability of these communities may depend on different factors and results in less marked species-specific differences. However, other studies [27,179] found clear differences between the leaf-associated and the seawater community, suggesting a host-driven selection in the shaping of the associated communities. Probably, both host-driven selection and random microbial recruitment simultaneously operate in determining the structure and composition of the microbiota. Hence, deepening our knowledge on the relative contribution of these components, with wide comparison studies in different seagrass host species and environmental conditions, will allow us to understand and predict whether and how an environmental change might affect the microbial community, the seagrass host or the entire holobiont.
The belowground associated microbial communities were found to be less variable than those associated with the aboveground plant compartment [24,25,27,31]. The colonization of the belowground plant compartment is assumed to depend on both the chemical microenvironments [29,89] and the age or density/structure of the roots [96,99,122]. Cúcio et al. [38] found similarities among seagrass rhizosphere colonizers in three seagrass species (Z. noltei, Z. marina and C. nodosa) from the same location (assumed to contain similar sediment type and conditions), suggesting that seagrass are able to select their epiphytes, as a functionally oriented and not a seagrass species-specific selection. On the contrary, Martin et al. [29] suggested that different seagrasses from the same site have different root microbiota due to seagrass the species-specific root exudates. Similarly, Garcias-Bonet et al. [175] found different bacterial communities in two different seagrass species rooted in the same site, suggesting that both the host-species and the environmental conditions contributed to shape the microbial community.
Species-specific root-associated microbial communities have been found also in the same seagrass species from different sites [24,25,120], leading to the suggestion that future studies might reveal a wide and potentially even a worldwide similarity among speciesspecific belowground microbial communities [120]. This hypothesis was confirmed by the H. stipulacea case study already discussed [24,25,179] (see Section 1.3), where a high incidence of Alphaproteobacteria were found in both native (Northern Gulf of Aqaba) [24,25] and invasive sites (Eastern Mediterranean Sea) [179]. These results might suggest that microbial colonization of seagrass roots could be mainly driven by host metabolism rather than by response to local environmental conditions [26,29,31,89,103].
These contrasting outcomes about the relations between microbes and seagrass species do not exclude each other. The microbial colonization of seagrass may follow different successive patterns, in which "suitable" bacteria are chosen among those available in the local pools (depending on environmental conditions) and act as pioneer colonizers. Some of these species can be promoted/hindered by the host-bacteria selection process, according to the different host traits [183,184]. Moreover, according to Ugarelli et al. [39], specific differences in microbial colonizers may be overlooked as the most abundant families (generalist heterotrophic groups) are shared by different seagrass species, while the speciesspecific associated microbes may represent a minor component of the associated microbial community.
Although they are physically and biochemically/physiologically interconnected, the above-and belowground plant parts are morphologically different and exposed to different conditions, thus it is assumed that they are differently colonized. In terrestrial plants, soil microbes colonize roots and then migrate to the aboveground parts [68]; this has never been assessed in seagrass, even if a bottom-up influence has been speculated. The link between the microbial communities of the two plant parts could be indirect, with microbes flowing with organic matter from the above-to the belowground plant part or into sediment; this could explain the shared microbial community components between the above-and belowground plant parts [100,125,185]. The link could be either direct: for instance, environmental changes acting on leaves may influence the physiological activity of roots, to which the microbial community promptly reacts [29,31,103].

Environmental Conditions
Seagrasses respond to the environmental variation by changing their physiological and biochemical activity followed by changes to their morphology. The associated microbial community seems to be sensitive to either the environmental or the host's physiological changes. Compared to the belowground microbial communities, the aboveground microbial epiphytes seems to be more sensitive to the environmental changes; this might be due to: (i) the possible instantaneous variation of the environmental conditions, to which the bacterial taxonomic assembly rapidly responds, as suggested for seaweeds [76,183,186], and/or (ii) event-driven patterns, which lower the probability that the first colonizers of the emerging leaf will be exactly the same for different leaf onset events [25]. Conversely, the belowground microbial epiphytes seem more conservative and sensitive to the host physiological changes [21].
Reduced light availability, for instance, alters seagrass root exudation that, in turn, changes the root microbiota composition, reducing the abundance of potentially beneficial microorganisms [29]. In roots, low light exposures increase the exudation of total dissolved organic carbon and nitrogen, as a result of fermentation products. This release is due to the metabolic shift under hypoxic condition, as belowground oxygen supply is limited by reduced photosynthetic activity [29,103]. Under natural conditions, light availability depends on seawater turbidity, but may also be affected by epiphyte coverage as well, as demonstrated by Brodersen et al. [103]: the leaf epiphyte coverage reduces the amount and quality of light radiation available for photosynthesis, reducing the plant fitness. Rotini et al. [31] showed a daily variation of the microbial community composition associated with Thalassia hemprichii: plants responded to changing irradiance with changing production of HSP60 and HSP70, and accordingly, the phyllosphere-associated microbial community shifts from Enterobacteriaceae (dominant in the morning) to photosynthetic Alphaproteobacteria (dominant in the afternoon); concurrently, a variation occurs also in the composition of the belowground microbial community.
Simulating ocean acidification, Hassenrück et al. [23] instead, analyzed the influence of the low pH on the microbial communities associated with Enhalus acoroides leaves due to natural CO 2 -vents. They found differences in the leaf associated microbial biofilm composition as a result of to the proximity of the vents, highlighting the sensitivity of leaf biofilm to environmental gradients (e.g., pH gradient) and due to different leaf ages, suggesting a potential influence of the host physiological conditions of the host, but unfortunately this last part is not discussed in the paper.
These studies highlight the coupled responses to the environmental changes of the seagrass physiology and composition of associated microbes, suggesting that this rapid microbial response deserves more attention, as it might be a sensitive and an early warning indicator of the changing plant physiological state.
In addition to being affected by their environments, seagrasses-defined as ecosystem engineers-are also able to modify their surroundings environments, including the microbial communities [84,89,95,[187][188][189][190][191][192]. Indeed, vegetated sediments host high abundance of nitrogen-fixing bacteria [95,189], and the seagrass rhizosphere hosts high abundance of microbial genes related to putative phosphorus and sulfur pathways [26,190]. In this perspective, the biogeochemical cycles are undoubtedly influenced by seagrass meadows, which, in turn, have been found to influence the sediment microbial taxonomic profile [96,97,99,191,192], with a larger extent than environmental factors like the seasonal seawater temperature fluctuation [21]. Ettinger et al. [96] found a significant correlation between sediment microbial community structure and seagrass density, with an enrichment of microbes involved in the sulfur-cycle in the densest area of the meadow. On the contrary, Bourque et al. [188] found that the complexity of the seagrass sediment microbial community seems to be influenced by the ecological condition of the seagrass meadows that was lower in the disturbed sediment, while, similarly Sun et al. [191] found that seagrasses caused a decrease in sediment microbial diversity. Another interesting insight is the observation that the microbial community within the sediment surrounding the native Zostera capricorni is able to hinder the growth of the invasive seaweed Caulerpa taxifolia, contributing to the capacity to resist the settling of this invasive macroalgal species [192].
While the mutual influences between seagrasses and the microbial communities in their surrounding sediments have been demonstrated [26,29,89,95,96,187,188,192], the relationship between seagrasses and their surrounding seawater column is less clear. Lamb et al. [193] found that the presence of seagrass reduced the potential microbial pathogens (e.g., Enterococcus spp.) in the water column and lowered the incidence of corals diseases in the coral reef neighboring these seagrass beds. However, Webb et al. [176] attributed the reduction of potential microbial pathogens within the seawater column to the location, current flow and tides (inside vs. outside the San Diego Bay), rather than to the presence of seagrasses. This discrepancy could reflect geographical differences in terms of contaminant exposure and water circulation.
Taken together, this data highlights the continuous dialogue between the abiotic factors and the living organisms' complex associations, suggesting that the microbial perspective should be considered in a much wider ecosystem scale and perspective.

Taxonomy and Functions
As a consequence of bacterial metabolic versatility, the taxonomic composition of the communities may be quite distinct despite their superimposable functionality. Furthermore, regardless of host species and conditions, the functional redundancy of the communities guarantees the key processes, which may be unaffected by changes in community structure [26,167,194]. Thus, plants may recruit microbial populations belonging to different taxa, among those available in the environment, ensuring the key functions for the holobiont [167].
However contextually, each seagrass host-type may own a specific taxonomic and functional microbial community, due to its physicochemical properties. To identify the influence of the host species on the composition of their associated microbes, Roth-Schulze et al. [194] compared surface-associated communities of different seagrass and seaweed species from different sites; they found that sample-type rather than collection time and location shaped the microbial communities, underlining the host influence in terms of taxonomic composition; however, they also found that despite the taxonomic differences, the microbial communities shared a wide range of functional capabilities. This suggests that seagrass epiphytic microbial community composition may depend on microbial functionality rather than taxonomy following the "lottery model" [195]. According to this model, there are several microbes that are able to play the same functional role, but during the colonization process the first colonizer is the one that eventually settles in the community [184,194,195]. This hypothesis was recently confirmed by Martin et al. [29] that compared the microbial community associated to seagrass roots under different light conditions and found a variation of taxonomic microbial composition, but no variation in the functional pathways of these communities. These results were made possible by using the 16S rRNA gene for the microbial taxonomic identification coupled with PICRUST software [196]. The PICRUST output gives a first insight into the functional profile of a microbial community [197]: it predicts the gene functionality of the community relating to the taxonomic profile produced by the 16S rRNA metagenomics, and the metabolic pathways of the identified taxa found into the KEGG database [198]. However, due to the paucity of available reference genomes in the database, and the huge genomic diversity of isolates belonging to the same species, it does not provide a reliable picture of the community functional profile [32].
In fact, the great majority of the studies on the seagrass associated microbial communities are based on their taxonomic identification based on 16S rDNA amplicon sequencing. Analysis of the 16S rRNA gene clearly highlights the shared and unique microbial components among seagrass species or sites, allowing one to infer host and/or conditions specificity in the frame of comparative studies. However, while this type of sequencing gives insights into the potential microbial functionality, it does not reveal the actual biochemical processes active at a given moment in the community [27]; furthermore, the 16S rRNA gene amplicon sequencing techniques usually do not reach a deep classification level, and therefore are not able to provide a reliable taxonomic identification, at least for some subsets of microbes.
To cope with these issues, multiple omics-techniques have to be applied, such as the use of shotgun meta-transcriptomics or proteomics, which could provide important information necessary to gain a wider and realistic overview of the seagrass-microbes interactions. The importance of available techniques and bioinformatics tools is discussed in the next paragraph (see Section 3).

Key Role of Methods in Characterizing the Seagrass Holobiont (from Classical to "Omics")
Only a small fraction of the bacterial species can be readily cultivated in microbiology laboratories, hence, studies assessing the composition of different microbial communities, including those growing on seagrass, have gradually moved away from the culture related approaches [37,105,199,200], to culture-independent molecular based techniques [35,36,99,161,162,201] and, ultimately, to next-generation sequencing (NGS) approaches [23][24][25][27][28][29]31,32,38,39,96,97,191,202]. This paradigm change has been necessary because bacterial growth is inhibited in vitro, if crucial factors of their natural environment are not properly replicated in the laboratory. While abiotic factors (e.g., pH, temperature, oxygen and nutrients availability, light intensities, etc.) can be quite easily replicated in vitro, biotic factors are much more difficult to mimic. In addition, evidence from culture dependent and microscopic approaches underscore a prominent role of fungi within seagrass physiology and ecology [203][204][205], but studies providing a wide overview of these taxa, such as those employing NGS approaches, are still incipient [27,80,206,207]. A similar paucity of information turns evident also considering the association of seagrass with viruses and archaea, even if, for the latter, the existence of both Euryarchaeota and Crenarchaeota in sediments associated with different Zostera spp. has been reported [203,206].
Therefore, studies aiming to decipher the complex interactions between members of a holobiont, more and more frequently employ a number of molecular based techniques, with NGS-based emerging for their ability to provide detailed snapshots of the complete structure and composition of microbial communities in a defined interval of space and time.
Studies using target amplicon sequencing, especially those focused on the ribosomal DNA (mainly 16S for prokaryotes, 28S or internal transcribed spacers (ITS) for fungi and 18S for other eukaryotes), play a major role in characterizing and deciphering the complex network of interactions occurring in the seagrass holobiont [78][79][80]. Even if this approach theoretically allows the precise identification of individual partners constituting a holobiont, and their functional potentials, there are several hurdles that yet need to be overcome. A major problem is the need of standardized methods during the whole experimental workflow, including: (i) sampling procedures, (ii) sample processing to obtain crude DNA extracts, (iii) amplification and sequencing steps and (iv) processing and analysis of raw data.
The sampling step is obviously strictly related to the aim of the study and to the specific questions the research team wants to answer. Therefore, sampling sites, sample collection methods and number of replicates must be correctly defined/chosen to reach a good level of statistical significance and detect the whole species diversity and abundance. Moreover, collecting the microbial biofilm from the seagrass tissue is not a standardized practice and it may influence the outcome of the study. For instance, in several studies samples were rinsed with sterile seawater [28,35,39,106,178,183,186,192,200] while this step was not applied in others [24,25,31,93,120]. In some cases, the microbial biofilm was collected by washing seagrass tissue with a hand-made solution [24,25,31], with sonication [39], from frozen seagrass samples [39], or directly using the seagrass tissues in the DNA extraction process [89], and even, in several studies, the procedure is not described in details.
The process to obtain DNA preparations for amplification and massive sequencing, by using different methods and commercial kits, is another key step that may introduce biases in the workflow for the characterization of the holobiont. This has been demonstrated for 16S rRNA gene profiling of microbiome samples processed with different DNA extraction protocols [208], or for whole-genome sequencing of complete communities by employing extraction kits from different manufacturers. Major variations in the obtained results were found to be related to the different extraction and sequencing methods applied [209]. In fact, even if the rapid development of alternative chemistries and processing approaches significantly improves data quality, the use of different polymerases (characterized by different performances for high %G + C DNA regions and different error rates) or the presence of differently abundant sequences from specific taxa in the sample, can introduce a certain degree of bias resulting in a poor-quality level of results. In these studies, the use of a cell spike-in control of known concentration during the nucleic acid extraction step has been suggested to alleviate such problems and would also allow a more accurate quantification of organisms based on a known input value [209]. Moreover, the use of PCRfree protocols (such as Kapa Hyper Prep PCR-free or TruSeq DNA PCR-free) is expected to reduce biases due to amplification and occurring in calculations of abundances. Regarding the instruments used for sequencing NGS libraries, with Roche's decision to discontinue their 454-sequencing platform (the first NGS commercial platforms on the market), the Ion Torrent Personal Genome Machine (PGM) and Illumina platforms are now the most used machines for studies characterizing the holobiont, with the prevalence of Illumina over PGM that has recently significantly increased [210]. This is largely due to the ability of the former platform to limit the number of sequencing errors, especially indels [211], even though Quail et al. [212] showed that the PGM platform showed better performances, but at the expense of a higher false positive rate. In fact, while the 16S fragments commonly used by Illumina include the V3-V4 hypervariable regions, determined by a paired-end sequencing approach on amplicons of approximately 460 bp, the PGM instrument allows one to obtain single, long reads covering the entire V1-V9 region, theoretically allowing better discrimination of the taxa present in a given sample.
Several software are available to analyze NGS data derived from microbiome investigations, with MOTHUR [213] and Quantitative Insights Into Microbial Ecology (QIIME) [214] being probably the most popular. Indeed, even if the software selection depends largely on the level of taxonomic depth and complexity to be reached, a study concluded that QIIME and MOTHUR were the most complete pipelines, with several features and thoroughly documented [215]. Additionally, to consider here is that some studies highlight the fact that the best software performances are related also to the specific NGS technology used in the experiment. In fact, although QIIME 1 is no longer supported (since the end of 2017), and was replaced by QIIME 2, a recent study that analyzed a mock specimen (an artificial known bacterial community) suggested that the best results are obtained using QIIME1 when PGM data are used, while when using data from Illumina the VSEARCH strategy, which can be implemented in QIIME, gave superior results [216].
Concerning the analysis step, the choice of an appropriate resource for taxonomic classification to decipher sequence information is fundamental to answer specific questions that are at the basis of a given investigation. The importance of this step was recently highlighted by the observations that not all resources can be mapped to others without conflicts, and that there is a great deal of difference between taxonomies that arise mainly for the differences in their size and structure [217].
Indeed, while three of the databases widely used in microbiome studies, i.e., SILVA [218], RDP [219] and NCBI Taxonomy [220], cover the complete domain spectrum of Bacteria, Archaea and Eukarya, other such as Greengenes [221] are limited to specific domains (Bacteria and Archaea). In addition, the taxonomic granularity that characterizes these resources is also different, with only Greengenes and NCBI Taxonomy reaching the species level, while SILVA and RDP allow the identification at the genus level. When working with these different resources, one should take into consideration that the use of gene subregions to identify taxa, often does not allow the discrimination between closely related taxa, which could require the complete sequencing of the ribosomal gene [222]. Lastly, while updated versions of SILVA and NCBI Taxonomy are available, Greengenes and RDP have not been updated since 2013 and 2016, respectively. These are just some of the differences among these resources, which have to be taken into account to perform the best selection for answering the biological question at the base of a given experiment.
As a final remark regarding the use of massive 16S rRNA sequencing for studying the seagrass holobiont, at the moment, the use of (i) commercial kits able to provide efficient DNA extractions characterized by a low level of contaminants, (ii) a sequencing platforms with low error rates in base calling, such as those of Illumina and (iii) a well-documented and widely used software as QIIME 2, could be proposed as a sort of "best practice" to investigate the microbiota of the seagrasses holobiont. Furthermore, sampling procedures should be described in details and already standardized/available methods should be preferred.
Beside these types of characterization, other omic-related techniques are gradually contributing to provide detailed data of the different constituents of the seagrass holobiont and their interactions. For example, a transcriptomic approach was applied to investigate the short-term responses in gene expression of seagrasses exposed to stress, as changing temperature [223] or light availability [224]. Metabolomic investigations demonstrated changes in nitrogen and sulfur metabolism, not detected by classical techniques [102]. The application of these and other novel approaches will contribute to a better and deeper understanding of the seagrasses holobiont, for instance by elucidating the gene expression pattern and the metabolic activities related to specific associations or environmental conditions.

Mutual Influence Seagrass/Microbes: Implication and Insights for the Development of an Integrated Index
Biological and ecological indicators represent useful tools for environmental monitoring, being crucial for the description and prediction of environmental changes [225]. The application of biological indicators in monitoring is recommended by the European Marine Strategy Framework Directive (2008/56/EC), which is based on an integrated "ecosystem" approach. In addition to the common ecological quality indicators (macrophytes, diatoms, macrobenthos and fish), changes in the microbial communities associated with seagrasses deserve to be taken into consideration as putative indicator for environmental quality assessment in marine ecosystems, due to their sensitivity and high turnover, and many efforts are made to develop environmental microbial indices [226]. Inspired by AZTI's marine biotic index (AMBI index; [227]) for the assessment of the benthic ecological quality, the micro-AMBI [228] is an index that examines the response of soft-bottom benthic microbial communities to natural and man-induced disturbances in coastal and estuarine sandy environments [229]. This index showed a significant correlation between the relative abundance of microbial taxa and the sediment quality index.
The epiphytic microbial communities associated with seagrasses are diverse may establish symbiotic relationships with the host and respond rapidly to environmental changes [24,33]. Thus, the structural and compositional variations of seagrass associatedmicrobiomes may be informative of the seagrass ecological status and a useful marker of specific stress that other seagrass health metrics fail to detect. For this reason, it is highly important to apply an integrated approach in the evaluation of the ecological status of seagrasses, that may be influenced and sequentially influence the associated microbial communities, or use different techniques to highlight the ongoing physiological processes [24,25,31].
Studies on seaweeds, more advanced than those on seagrass and reviewed elsewhere [72][73][74], revealed that in addition to host species and local conditions [183,230,231], the host health condition plays a pivotal role in determining the seaweed holobiont composition [57,76,77]. Marzinelli et al. [76], on a continental scale study, found that healthy organisms have different microbial pattern, geographically determined; on the contrary, stressed plants exhibit similar microbial colonizers across sites. Longford et al. [57] showed that the microbial community associated to a host changes following a disturbance, and may recover to the prestress state after the end of the disturbance. This give evidence that interactions within microbiomes have important implications for host health and disease resilience. Minich et al. [77] found that the kelp and the associated community responded in a stress-specific way to the environmental changes (elevated temperature and pCO 2 ), highlighting the interactions among the host and the associated microbiota.
The relationship between the health condition of seagrass meadows and microbiota composition are still incipient [55], but studies that related seagrass descriptors and the epiphytic microbial community variation proposed microbes as putative markers [22,24,25,31,32], as seagrass associated microbial communities were found to change even at the small spatial scale in different environments [24,25].
Variation of the microbial epiphytic communities associated with H. stipulacea was found along with seagrass morphological and biochemical markers (i.e., pigments and phenols content [24,25]); different microbial groups characterized healthy or stressed seagrass meadows [21,22,32,122] and freshwater plants meadows [232]. Earlier studies that focused on P. oceanica [21,22,122] found the dominance of certain bacterial groups in a declining meadow, such as Pseudoalteromonas [122] and sulfate-reducing bacteria and the increase of sulfide [21]. Martin et al. [32] combined 16S rRNA gene sequencing of the seagrass root microbiome of Halophila ovalis with seagrass health metrics (biomass, productivity and sulfide concentration into seagrass tissue) and found that groups of putative methylotrophic bacteria, iron cycling bacteria and N 2 fixing bacteria were associated with "healthy" seagrasses, while "stressed" seagrasses were dominated by putative sulfur-cycling bacteria, both sulfide-oxidizing and sulphate-reducing.
A detailed overview of the studies that suggest and/or support the use of microbes as ecological indicators is summarized in the following table (Table 1).  The ecological and health condition of seagrass host seems to be an important shaping factor of the associated microbial communities and of the overall seagrass holobiont. Furthermore, the mutual influence between seagrass and environmental microbial communities, although is still little explored, suggesting that the seagrass associated-microbes could be a tool to monitor the environmental changes and the seagrass ecological status and, potentially, their manipulation could be useful to preserve and/or restore seagrass ecosystems. This might allow us to prevent the ongoing decline of seagrass meadows and, potentially, the invasiveness of exotic species, and to control the spread of pathogens both from humans and from marine organisms.
Here we list some of the main aspects that should be considered and further investigated in order to define early warning indicators of seagrass health and to develop, hopefully, in the near future, an integrated microbial community-based index.
(a) Occurrence and relative abundance of specific bacterial groups (taxonomy) and/or genes or metabolic pathways (functionality) can be putative markers, particularly those involved in important biogeochemical transformations within the nitrogen, sulfur and carbon cycles and thus able to affect the ecology/physiology of the seagrass host. (b) Abundance pattern and contributions of rare vs. abundant components of the holobiont microbial partner into the integrity of the seagrass host health status and the related ecological processes. For example, the rare microbial components can have greater sensitivity to both natural-and human-induced disturbances and/or exert significant impacts on seagrass ecosystem health, plasticity or resilience. Similarly, the core microbiome and the changing components of the seagrass associated microbial communities can give a different contribution to the functional structure of the microbial assemblages and thus to the ecological status of the seagrass holobiont. However, instead of a universally important seagrass-microbial core, a local or species-specific microbial core might be useful to identify. (c) Differences and roles of the microbiomes associated with the different anatomic plant compartments, which can reflect and be related to the characteristics of the environmental matrices (sediment vs. seawater). For example, the belowground compartment seems to be an extremely conservative microenvironment for seagrass associated microbial community, showing good sensitivity to the seagrass physiological condition and responding to the environmental conditions; the aboveground microbial community, instead, showed higher variability and seemed more easily influenced by the seawater conditions. Thus, the belowground microbial community may be suitable to monitor the seagrass ecological status while the aboveground microbial community may track the environmental changes, making seagrass leaves a good reference surface to monitor environmental variations or disturbances. (d) Plant surface colonization patterns/pathways and ecological interactions occurring within the seagrass epiphytic microbial community can be complex and have important implications for the holobiont health and disease (e.g., symbiosis, competition, predation, antifouling and antimicrobial activities); thus, they deserve attention and further investigation to give an insight of the host-microbial functional relationships.

Conclusions
The possible methodological approaches based on the use of microbes (or microbiomes) as bioindicators in the marine environment monitoring are several, however standardized methods or at least a proposal of "best practices" should be defined (including the sample collection and processing and the raw data analyses) for seagrass holobiont investigations (see Section 3). Due to genomic and metagenomic advances, the seagrass associated microbial communities are increasingly known and it is now possible to study their role and interactions with the host plant. There is still much to do in this field, but already now the microbiome represents a challenge for ecotoxicology, to exploit the potential development of ecological/ecotoxicological indices. Field and mesocosm experiments by using an integrated approach that combine microbiome analysis and seagrass descriptors are recommended to investigate the response of the holobiont to physical-chemical variations and environmental disturbances.