Review on Emerging Waterborne Pathogens in Africa: The Case of Cryptosporidium

Water-related diseases, particularly waterborne diseases, remain significant sources of morbidity and mortality worldwide but especially in developing countries. Emerging waterborne pathogens represent a major health risk. Cryptosporidium is one such pathogen which is globally recognized as a major cause of diarrhea in children and adults. The objective of this paper is not only to review published studies on the impact of these emerging waterborne pathogens but also to identify the various risk factors that favor their transmission. A number of envisaged and needed actions to tackle the challenge of these pathogens in Africa have also been discussed. We have searched the web of ScienceDirect, PubMed, Scopus. ISI Web of Science, SpringerLink, and Google Scholar. The first database search yielded 3099 articles. As a result, 141 studies were submitted for abstract review. A total of 68 articles were selected for full text analysis. After evaluating a considerable number of articles on this topic, the following results were obtained. A number of pathogens are likely to present public health risks, including Cryptosporidium. The contaminating potential of these pathogens is associated with a multitude of factors, such as the effects of climate change, social and behavioral aspects of local populations, water issues, geographical locations that may cause isolation, and inequalities due to lack of transparency of governments in the distribution of financial resources. We stress the need to maintain and strengthen real-time surveillance and rapid epidemiological responses to outbreaks and the emergence of new waterborne pathogens in all countries. African governments, for their part, should be aware of future risks of waterborne protozoan diseases. They must provide immediate and effective responses by establishing technical and financial mechanisms to ensure sufficient quantities of safe drinking water, sewage disposal, and hygiene for all.


Introduction
Diarrheal diseases have been classified as the fourth leading cause of death in children, after iron deficiency anemia, skin diseases, and protein-energy malnutrition. In Africa, the World Health Organization (WHO) has classified diarrheal diseases of viral, bacterial, and parasitic origin as leading causes of human mortality [1]. The Institute of Medicine declared in 1992 that an emerging infection is any new, re-emerging or drug-resistant infection that is likely to increase in frequency in the near future [2]. A large number of publications and journals on the topic address the issue of emerging and re-emerging pathogens in general, including waterborne pathogens, without addressing issues specifically related to emerging waterborne pathogens. Although responsible for waterborne epidemics worldwide, the disease burden due to Cryptosporidium is highest in Africa [3][4][5]. These pathogenic microorganisms are likely to be transmitted through contaminated drinking water, including both treated and untreated wastewater [6]. Surface waters are frequently polluted by unregulated wastewater discharges, poorly controlled wastewater treatment facilities, and inadequate waste disposal facilities in residential areas near rivers [7,8].
How has the relationship between waterborne diseases and water consumption been analyzed in Africa?
Are there any overlooked water consumption parameters, waterborne syndromes or diseases?
What are the causes of the spread of these diseases in Africa?

Scope and Strategies of the Current Review
A methodical approach was used for this review, including: (i) a systematic review of the literature to identify articles that met the inclusion criteria; (ii) the relevance of the articles to the study themes; (iii) the performance of full-text analyses of the articles; and (iv) the extraction and synthesis of results. The general protocol for the scoping review was inspired by various previous studies. The main research questions guiding this review ere: "what is the prevalence of waterborne diseases in Africa in general?", "what are the most commonly reported sources/pathways of contamination?", and "what detection methods can be used for its identification?".

Inclusion and Exclusion Criteria
The selection of the main search keywords was based on a modified version of the population outcome agent (POA) model, including concrete search strategies used to conduct structured literature searches [16]. Searches conducted using a different category of "Result" search term (e.g., Cryptosporidiosis, contamination, epidemic, etc.) fell considerably short of the number of articles identified. Therefore, the search terms of the categories "agent" and "outcome" were combined to broaden the search field. The databases were searched using Boolean operators ("AND", "OR", "WITH", "SAME", "ADJ") to focus the literature review. To analyze the relevance of the different studies included in the literature review, and to assess their quality, we used the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) checklist [17]. (See Table 1; the list of the variables extracted from the articles during the selection process). Self-reported/diagnosed/laboratory confirmed Cyclosporiasis Self-reported/diagnosed/laboratory confirmed Gastroenteritis Self-reported/diagnosed/laboratory confirmed Cryptosporidium Self-reported/diagnosed/laboratory confirmed Cryptosporidiosis Self-reported/diagnosed/laboratory confirmed

Characteristics of the Publications
The first database search yielded 3099 articles. After removing duplicates and assessing abstracts for eligibility, only 141 full-text articles remained to be reviewed, 68 of which met the inclusion criteria.
Based on our checklist, we identified the strengths and weaknesses of the studies. All articles had a specific objective, a study design, and a study site with a date for data collection. Most of the selected studies clearly indicated the selection criteria ( Table 2). The target population of the studies was households in general, which confirmed cases Water 2021, 13, 2966 4 of 17 or patients in hospitals treated for waterborne diseases, and focused on children under 10 years of age, as well as women, mothers of infants, and HIV-infected persons.  [8,17,23,29,34,77] The results of this synthesis provide an overview of the impact of waterborne diseases in Africa, including cryptosporidiosis caused by emerging pathogens. It highlights currently available information on the epidemiology of Cryptosporidium, as well as its treatment and distribution on the African continent, particularly among vulnerable populations including children. It also highlights risk factors to enable African governments to address current and future challenges.

Cryptosporidium
As a result of the lack of sporocysts in the oocyst (i.e., naked sporozoites), Cryptosporidium has been considered enigmatic since it was described for the first time by Edward Tyzzer in 1907 from the gastric glands of a mouse [18]. He placed it in the coccidian family Asporocystidae because of the oocyst's lack of sporocysts (i.e., sporozoites) and what was presumed to be the possession of similar life cycle characteristics [19]. In the 70 years since Tyzzer's description, this Cryptosporidium has been regarded as a curiosity. More species were described, mainly on the basis of the presence of hosts, but this parasite was always seen as atypical. These biological characteristics, however, were overshadowed by the serious public health implications of the opportunistic Cryptosporidium infections that emerged in the 1980s, mainly by taking advantage of the weakened immune systems of AIDS patients [20]. This health emergence highlighted the use of chemotherapies and the early evidence that Cryptosporidium is completely insensitive to anticoccidial drugs [21,22]. Numerous species of Cryptosporidium exist which infect humans and a large number of animals. Although Cryptosporidium parvum and Cryptosporidium hominis (formerly known as the anthroponotic genotype or genotype 1 of C. parvum) are the main organisms causing disease in humans, infections with C. felis, C. meleagridis, C. canis and C. muris have also been reported. Infections with Cryptosporidium parvum and C. hominis species are mainly through water contamination (e.g., drinking or recreational water). Occasionally, certain foods, such as chicken salad, are likely to promote transmission. Direct faecal/oral spread is likely to be the most common form of transmission, both for zoonotic diseases and for direct transmission from parent to child [23,24]. Waterborne epidemics are a major issue worldwide. They are a financial burden for water companies. Indeed, it has been revealed that the phenomenon is amplified by the fact that biofilms constitute Cryptosporidium reservoirs in which oocysts are not only trapped and then released into the water distribution system, but also highly nutritious environments allowing the parasite to grow and reproduce [25,26] (Figure 1). hominis (formerly known as the anthroponotic genotype or genotype 1 of C. parvum) are the main organisms causing disease in humans, infections with C. felis, C. meleagridis, C. canis and C. muris have also been reported. Infections with Cryptosporidium parvum and C. hominis species are mainly through water contamination (e.g., drinking or recreational water). Occasionally, certain foods, such as chicken salad, are likely to promote transmission. Direct faecal/oral spread is likely to be the most common form of transmission, both for zoonotic diseases and for direct transmission from parent to child [23,24]. Waterborne epidemics are a major issue worldwide. They are a financial burden for water companies. Indeed, it has been revealed that the phenomenon is amplified by the fact that biofilms constitute Cryptosporidium reservoirs in which oocysts are not only trapped and then released into the water distribution system, but also highly nutritious environments allowing the parasite to grow and reproduce [25,26] (Figure 1).
In developing and developed countries, the spread of parasites by waterborne means is therefore likely to be a consequence of contaminated or malfunctioning water facilities. Infections by indirect transmission, i.e., by uncontrolled transmission of oocysts from flies [27] or other animals, such as dogs or cattle, or through contaminated food or local water sources. They are a real danger, especially in developing countries [28,29]. Rural areas are at greater risk of infection than urban areas, probably due to the higher risk of direct and indirect contamination in areas with poor hygiene and higher rates of contact with wildlife and pets [30]. Poor hygiene is a key factor in the transmission of enteric protozoa, such as Cryptosporidium [30].  [26] for methods). Scale bar = 3 µm.
In developing and developed countries, the spread of parasites by waterborne means is therefore likely to be a consequence of contaminated or malfunctioning water facilities. Infections by indirect transmission, i.e., by uncontrolled transmission of oocysts from flies [27] or other animals, such as dogs or cattle, or through contaminated food or local water sources. They are a real danger, especially in developing countries [28,29]. Rural areas are at greater risk of infection than urban areas, probably due to the higher risk of direct and indirect contamination in areas with poor hygiene and higher rates of contact with wildlife and pets [30]. Poor hygiene is a key factor in the transmission of enteric protozoa, such as Cryptosporidium [30].

Emerging Water-Borne Pathogens
In order to better understand the causes of emergence, a distinction must be made between emerging waterborne pathogens and newly recognized pathogens. The majority of emerging waterborne pathogens fall into the former category, implying that, although the etiological agent has been known for a very long time, it has only recently been recognized as the main cause of waterborne diseases. These include parasitic protozoa, such as Cryptosporidium Microsporidia; Campylobacter; several viruses, including caliciviruses and hepatitis E virus; and a multitude of environmental bacteria, namely Mycobacterium, aeromonads, Pneumophila, and aeruginosa. There are several possible reasons for the emergence of newly recognized pathogens. One of the most important reasons, which probably concerns everyone, is the development of efficient detection methods, including molecular, immunological, and immunomagnetic techniques [31]. Another, perhaps more important, reason for the emergence of Cryptosporidium is the increase in the number of immunocompromised people, such as patients receiving cancer treatment or organ transplants, the elderly, and AIDS patients. The emergence of these pathogens is probably related to the relatively greater numbers in immunocompromised among the population. The growth of urbanization, which requires the use of large drinking water systems and its resulting issues, is mainly responsible for the emergence of waterborne diseases. Recently, the increasing migration of humans from one region to another has proven to introduce pathogens as well [32].

Human Health Impacts
Cryptosporidiosis has a very high impact in the majority of lower-GDP countries in the continent. Guinea-Bissau was one of the first states in Africa where the impact of Cryptosporidium was examined. It confirmed that Cryptosporidium was a cause of excess mortality in children under 12 months of age and that this excessive mortality rate continued into the second year of life. Although cryptosporidiosis was considered to have such an impact in some countries, the first comprehensive information confirming this has recently been provided, based on the results of the Global Burden of Disease (GBD) and the Global Enteric Multicentre Study (GEMS), e.g., [4,33]. These various studies have generated the first global estimates of the impact of cryptosporidiosis (among other diseases) ( Table 3).
An important analysis was conducted in 2018, revealing that previous reports were likely to have underestimated the high burden by not taking into account impacts occurring after the acute phase of infection, such as reduced growth, weight gain, and an increased risk of subsequent episodes of infection [34]. Other studies have also shown how Cryptosporidium diarrhea can damage the endothelial cells and microvilli of the gut, thus impairing the ability to absorb macronutrients and micronutrients [35]. Malnutrition caused by Cryptosporidium also causes secondary impairment of cell mediated immunity also linked to increased susceptibility to other infectious diseases. Other longterm consequences include reduced cognitive development, poor school performance, and increased risk of cardiovascular and metabolic diseases later in life [36,37]. Cryptosporidium infection in under-fives can be considered to be associated with 44.8 million diarrheal episodes and 48,300 deaths worldwide [38]. The majority of cases are in Africa, where 75% of diarrheal episodes and 88% of deaths have been recorded [33]. With regard to Cryptosporidium-associated diarrhea, the proportion of Cryptosporidium-associated deaths in children under five years of age is highest in sub-Saharan Africa, particularly in Nigeria and the Democratic Republic of Congo (DRC) [39]. By including the consequences of growth failure associated with cryptosporidiosis, it has been estimated that the parasite burden may be higher than previously observed [39].

Veterinary Health Impacts
Cryptosporidium infection in ruminants in general and infected young animals is highly likely to develop watery diarrhea and dehydration; death is common, especially in neonates. It is very difficult to ensure that exposure to an infectious dose in young animals is controlled [11]. It has also been shown that severe cryptosporidiosis in calves and lambs is likely to have long-term impacts on growth, weight gain, and productivity, in addition to the direct impact of acute infection, as in humans [40]. Since several other etiological agents besides Cryptosporidium can cause diarrhea in calves (e.g., rotavirus; coronavirus; bovine viral diarrhea virus; E. Coli; Clostridium perfringens; Salmonella spp.; and coccidia, such as Eimeria Zuernii and E. Bovis), these data do not indicate the presence of cryptosporidiosis [41].
Some researchers report that Cryptosporidium oocysts are very regularly identified in diarrheic calves in various African countries [42]. It is not clear though whether cryptosporidiosis is the cause of those symptoms. Many of these investigations use the modified Ziehl-Neelsen (mZn) test for determination. This type of test has a relatively low sensitivity but also a high specificity that does not identify the Cryptosporidium species. For example, in the context of a survey in Tanzania, researchers also used antibody immunofluorescence (IFAT) and molecular techniques to test for Cryptosporidium infection in calves. The results of these tests did not show the presence of Cryptosporidium in any of the 943 calf samples, of which more than 6% had diarrhea, while some of the results obtained with mZn were positive [43]. For these researchers, the information gathered with mZn should be treated with great caution. In Egypt, another project was conducted using molecular tools and found a 32% prevalence of Cryptosporidium in cattle [44]. A study in two large dairy farms in central Ethiopia showed that Cryptosporidium infection is common (40% cumulative incidence), with C. Parvum being mainly observed in pre-weaned calves and C. Andersoni in post-weaned calves [44]. All of these are likely to have a disproportionate impact on populations in developing countries [45]. Cryptosporidium are widespread in pets (Table 4), as well as in the environment and food crops, but also in people in Africa, with molecular typing revealing anthroponotic (human-to-human) contamination [13].

Incidence
The prevalence of cryptosporidiosis in children is highly variable in sub-Saharan Africa and in certain sectors of the population. Figure 2 summarizes the prevalence of cryptosporidiosis in children under 5 years of age who visited health facilities with diarrhea [46,47].Although diagnosis is rarely made in this setting, coinfection with other enteric pathogens is common, with common exposure through poor sanitation and hygiene and as a result of immune predisposition due to HIV infection. In most sub-Saharan countries, the prevalence of cryptosporidiosis peaks in children aged 6-12 months and declines thereafter. It can be assumed that breast feeding provides a form of protection, by conferring immunoglobin or preventing water contamination [48]. This may explain why infection is delayed until after the age of 6 months, an age that is usually characterized by the onset of complementary feeding. Children are often infected throughout their childhood and adolescence, while their clinical impact reduces with age [48]. This is probably due to the development of immunity following repeated exposure to oocysts in the contaminated environment [49]. Experimental research shows that repeated exposure to Cryptosporidium parvum results in an IgG response that offers partial protection against subsequent infection and disease [50]. The extent to which this serological response protects against infection by other Cryptosporidium species is still unknown. Epidemiological scenarios that promote and exacerbate Cryptosporidium infections are mainly access to safe water, prevalence of HIV infections, and malnutrition [45][46][47].
the development of immunity following repeated exposure to oocysts in the contaminated environment [49]. Experimental research shows that repeated exposure to Cryptosporidium parvum results in an IgG response that offers partial protection against subsequent infection and disease [50]. The extent to which this serological response protects against infection by other Cryptosporidium species is still unknown. Epidemiological scenarios that promote and exacerbate Cryptosporidium infections are mainly access to safe water, prevalence of HIV infections, and malnutrition [45][46][47]

Recognition and Diagnosis
The recognition and diagnosis of cryptosporidium has been totally revolutionized by the use of sophisticated techniques and methodologies, such as next-generation sequencing, whole-genome sequencing, and digital PCR. With these techniques, it is now easier to better understand microbial environments and their genetic composition, and to carry out counts on scales previously unimaginable. These methods, already widely used for bacteria, have also been applied to the determination and characterization of protozoa

Recognition and Diagnosis
The recognition and diagnosis of Cryptosporidium has been totally revolutionized by the use of sophisticated techniques and methodologies, such as next-generation sequencing, whole-genome sequencing, and digital PCR. With these techniques, it is now easier to better understand microbial environments and their genetic composition, and to carry out counts on scales previously unimaginable. These methods, already widely used for bacteria, have also been applied to the determination and characterization of protozoa such as Cryptosporidium. For example, in patients with dysentery, diagnosis of C. parvum is usually made using acid-fast staining or immunofluorescence on deconcentrated fecal smears. Good concentration methods can enhance detection when oocytes are in small numbers, but some methods, such as formalin acetate concentration, can cause the loss of many oocysts [52]. Although there are many enzymes linked to immunosorbent assay methods which detect fecal Cryptosporidium antigen with 83-95% sensitivity in diarrheal samples, they are less sensitive in constituted samples and require more time. The use of immunofluorescence antibody microscopy is slightly more effective and faster [53]. Polymerase chain reaction (PCR) is used to diagnose Cryptosporidium in water supplies or in asymptomatic carriers. The popularity of PCR has risen sharply in recent years due to its great potential to directly determine microbial species, including Cryptosporidium [52]. In fact, these technologies, which are fueled by recent advances in microfluidics, are involved in determining the absolute quantification of nucleic acid in samples without the need for large sample volumes and calibration [54]. Despite the remarkable success of this technology, it has been shown to be twice as expensive (consumables and labor) as PCR for the determination and quantification of Cryptosporidium [55]. This would therefore represent a significant problem for its application in most African countries, where financial resources are very limited. Although microscopy is the most widely used technique for Cryptosporidium identification, with immunofluorescence remaining the gold standard [56], this technique cannot distinguish between strains of these organisms or fully characterize the specific strains associated with outbreaks. This issue has been addressed by whole genome sequencing (WGS). Several experts have used WGS to elucidate the complete Cryptosporidium genome in samples of various types [55,57]. One of the main challenges of WGS is the ability to isolate sufficient DNA of very high quality [52]. This can be a real challenge when dealing with environmental samples, as these parasites are not widespread in the environment in general. Furthermore, WGS has not been widely applied to microbial species in Africa, probably due to its cost. These instruments are only available in some African countries. In addition, many of the studies that have been conducted using this technique have been carried out in partnership with other countries in Europe and America, where the equipment and technical know-how are available.

Treatment of Cryptosporidiosis in Africa
It should be noted that there is currently no effective vaccine against Cryptosporidium and that only one drug, Nitazoxanide (NTZ, Alinia; Romark Laboratories, Tampa, Florida, USA), is available to control Cryptosporidium. This treatment is not recommended for children under one year of age. Nitazoxanide has also been shown to reduce the resistance capacity of the parasite and may be useful in immunocompetent individuals [58]. However, the drug is not very effective against cryptosporidiosis in immunocompromised and HIVpositive individuals [59]. Nitazoxanide is therefore ineffective against the most critical target population in Africa, which has over 25.7 million HIV-infected people [13]. In HIV-coinfected individuals, antiretroviral therapy (ART) has been effective in controlling chronic diarrhea and wasting caused by cryptosporidiosis [60,61]. Today, supportive care and ART (for HIV/AIDS patients) form the basis of treatment for cryptosporidiosis. The main challenge for new drug development is therefore the underlying economic factor, as both parasitic infections are classified as neglected diseases with low funding priority and limited commercial interest [62]. For this reason, there is a movement to repurpose current treatments for unlicensed applications, as repurposed drugs are approximately 60% cheaper to bring to market than de novo developed drugs [62].

Discussion
We observe in this analysis that the majority of the surveys followed an environmental event riven design, with only four studies being motivated by gastrointestinal disease epidemics. All epidemic-related investigations were conducted in South-Saharan Africa, with most studies based in typical rural settings, followed by urban and mixed settings. The vast majority of studies have focused on the mode of waterborne transmission of emerging pathogenic microorganisms. We have noted that the reasons for the emergence of waterborne pathogens can, at best, only be addressed in terms of possibilities. Unequivocal answers require further investigation. There are several reasons for the emergence of newly recognized pathogens. One of the most important reasons, which is probably of concern to all, is the development of efficient detection methods, including molecular, immunological, and immunomagnetic techniques [31]. Other reasons, more directly related to the emergence of Cryptosporidium, include the ever-increasing number of immunocompromised people, for example patients undergoing cancer treatment or organ transplants, the elderly, people with AIDS, and the malnourished. The influence of sustained climate change on the emergence of waterborne pathogens should be taken into account.
Water is considered to be the main source of contamination, but the results of this study dispute this, as there is no evidence that the water supply is adequate in urban areas. Daily per capita water consumption and mode of access are inversely related to the level of health and safety hazards [63]. Prevention and control methods should aim to minimize and/or stop the transmission of infective oocysts to people and animals [64]. The establishment of wet sites to treat wastewater and runoff from confined animal husbandry has been shown to be not only a very effective means of eliminating pathogenic microorganisms, such as Cryptosporidium spp., but also very economical [65]. People at risk should avoid certain activities that are likely to increase their exposure to Cryptosporidium spp. Hand washing, education and treatment of water at the point of use (especially in Africa), boiling water before drinking, drinking safe water, and using filtration devices can minimize and significantly reduce the rate of transmission of infectious oocysts and the frequency of diarrhea. Exposure to feces during sexual intercourse should also be avoided [66]. Partnership projects to develop practical, effective, and innovative strategies are a key means of addressing these challenges and making progress on innovative strategies to stop the spread of Cryptosporidium in resource limited settings. In this context, scientists from the University of Virginia in Charlottesville, Virginia, USA, and the University of Venda in Limpopo Province, South Africa have teamed up to develop innovative, practical, and cost-effective strategies to control waterborne diseases. This program has led to a fruitful exchange of knowledge and expertise, resulting in innovative approaches to solving the most challenging problems in developing countries, such as the detection and disinfection of C. parvum oocysts in drinking water. Researchers in this collaborative program have revealed the disinfectant properties of silver towards C. parvum oocysts [67]. In addition, the researchers developed new laboratory methods to quantify the viability of oocysts, which, until now, was only determined by qualitative microscopic analysis of fluorogenic and excystation tests [68]. Finally, they developed and tested a new low-cost porous ceramic tablet comprising silver nanopatches for domestic water purification in resource-limited environments [34].
Further research, including studies on human health or animal infectivity models, is of course required to clearly determine the degree of efficacy against cryptosporidiosis, as the WHIL researchers plan to do. However, the preliminary results obtained are very satisfactory. Within the WHIL program, researchers have been able to exchange research and know-how between different specialties, and thus develop new disinfectants and innovative methods to determine the viability of oocysts and stop the spread of Cryptosporidium by developing new water purification technologies. Despite these advances, Cryptosporidium remains a major problem in resource-limited areas, particularly in Africa. To stop the transmission of Cryptosporidium in these areas, it is essential to address the risk factors for waterborne diseases [66,69].

Factors of Water Contamination
A growing body of research has revealed the presence of Cryptosporidium oocysts in all kinds of waterways, including lakes, rivers, wastewater, treated effluent, and drinking water in Africa, indicating the likelihood of endemic transmission of Cryptosporidium through water. The main causes of water pollution include uncontrolled wastewater discharges, poorly managed sewage treatment plants, and contaminated runoff from densely populated areas and unhygienic settlements along rivers [8,46]. The Kathita and Kiina rivers in Kenya, well water from the Kano River in Nigeria, surface water from the Vaal Dam, treated effluent and drinking water in South Africa, and piped water in Zambia have all been found to be contaminated by Cryptosporidium oocysts. High contamination levels of Cryptosporidium Spp. in river water; water for irrigation in Kumasi, Ghana; and vegetables consumed raw highlight the potential risk of cryptosporidiosis [13,33]. Ponds, streams, wells, and tap water in Ghana; tap water in Egypt; springs, wells, tap water, and dam water in Ethiopia; unsecured streams and wells in Malawi; open water sources (e.g., ponds or streams) in Uganda; and running water in Zambia have been reported to be contaminated with this pathogen [13]. Considering that water bodies in Africa are used by both humans and domesticated and wild animals, the risk of contamination of surface waters by Cryptosporidium excretion from these different entities is greatly increased. The fact that human and animal isolates can survive for a long period of time in all environmental waters poses a considerable health threat to local populations associated with the use of these receiving water bodies for drinking, recreation and agriculture when the quality of streams and tap water supplied to homes is unsafe, poor or unacceptable for human consumption [64].

Factors Relating to Hazardous Practices
A number of articles refer to risky behaviors that lead to the contamination of water systems through people's fecal waste, thus causing protozoan threats. Uncontrolled sewage dumping is a major risk that contributes to the prevalence of protozoa in water sources. In Uganda, the discharge of sewage directly into water bodies by hotels and fishing communities, the use of shallow toilets, and the discharge of wild and domestic animal excreta from the land into the water are all factors responsible for the contamination of natural water bodies (rivers, lakes, and canals) and tap water supplies with parasitic Amoeba and Cryptosporidium Spp. in ground defecation, sewage disposal, and the use of pit toilets, which are the main means of human-to-human spread of oocysts [68]. Half of the African population, particularly in sub-Saharan Africa, still use pit latrines as a method of excreta disposal, especially in developing countries [70]. The lack of waste treatment systems puts people at risk of severe gastroenteritis [71]. The consumption of spring water without any prior treatment is also a common practice among the Cameroonian population. In Yaunde Cameroon, the results of studies on water pollution in the Mingoa River (springs and wells) also confirm the influence of widespread urbanization and the practice of traditional pit toilets on the health of the population, as well as its relationship with the 40% prevalence of diarrhea and parasitic infestation [71]. As human activities increase, the water supply will be inadequate and sanitation facilities will be insufficient. Rapid population growth has led to the pollution of municipal lakes with a high accumulation of Cryptosporidium.

Climate Change Factor
While climate change may influence microbiological water quality and water-related disease, studies show considerable uncertainty about the nature of particular impacts, and when and where they will be most apparent. However, in order to assess the likely consequences for human health, it is essential to examine society's resilience to changing water quality. Understanding these adaptive potentials is crucial to determining the likely influence of climate change [72]. Climate change has a number of potential influences on water conditioning, and it is known that higher temperatures favor the implementation of biological water treatment methods [73]. A study on the impacts of climate change on surface water contamination found that climate change is likely to increase the risk associated with drinking water consumption, particularly during extreme weather events [73,74]. It was argued that the pathogenic risk would increase mainly due to higher temperatures and extreme precipitation events. Climate change poses a serious threat to the security of access to drinking water in Africa [75]. Increasingly erratic rainfall patterns are likely to affect freshwater supplies. Some parts of Africa have already been drier for a century (e.g., the Sahel) and, in the coming years, climate change is likely to expand the area affected by drought, doubling the frequency of extreme droughts and increasing their average duration by a factor of six [13]. Climate change will also increase malnutrition problems in Africa, as it will lead to changes in crop yields, higher food prices, and, thus, reduced food availability, reduced calorie availability, and increased child malnutrition in Africa. Cryptosporidium levels in African surface waters may also increase with population growth [13].

Sociological and Economic Factors
Poverty and illiteracy rates prevent people from being aware of the seriousness of untreated water consumption and domestic use, and the dangers of some of the practices that facilitate the transmission of diarrheal diseases [76]. As the phenomenon of large families is widely present in the African community, the demography has increased considerably, reducing the financial means per person and per family [77]. As a result, malnutrition is increasing in families, with children being the target. The lack of health education and personal hygiene programs leads to an increase in protozoan infections and the proliferation of diarrheal diseases [13].
Lack of sanitation and hygiene; reliance on open defecation practices; and the nonexistence of practices, such as handwashing with soap, all put children at increased risk. In Ethiopia, for example, only 19.5 per cent of Ethiopian households have access to sanitation facilities, while 80.5 per cent of households lack good hygiene practices [78]. In conclusion, poor families remain unhealthy, leading to economic instability and social marginalization [28]. Sociological and economic issues are important risk factors for contracting diarrheal diseases caused by Cryptosporidium [38].

Factors Relating to the Political System
The percentage of transmissibility of the parasite in Africa still remain a concern unless control and management measures are put in place, or important political and financial decisions are taken to make the surveillance system more effective and efficient. The political system is a key factor in the management and protection of public health in Africa. However, African leaders are unable to find a compromise to meet the challenges of building appropriate sanitation and hygiene infrastructure. There is a severe shortage of qualified health professionals and laboratories. African populations also lack transport and communication infrastructure [76]. As a result of these shortcomings, many patients have problems seeking treatment and have no choice but to resort to traditional remedies to cure diarrhea. Such practices certainly prevent accurate diagnosis and underestimate the prevalence of infectious diseases. Civil conflict and government corruption are factors that affect the long-term health of populations. These factors result in loss of life, especially among children and women [79]. Violent dictatorial regimes, conflicts, and wars in Africa, as well as unfulfilled political goals, have disrupted the health and education system and increased the prevalence of gastrointestinal diseases. Under conditions of corruption and war, agricultural facilities are lacking, resulting in food and water rationing. As a result, chronic hunger and malnutrition develop, leading to increased morbidity and mortality [80]. These factors are beyond the scope of the scientific community or individuals. Policy makers need to establish more humane rules and be more conscious of their decisions in order to justify and ensure a fair distribution of financial resources.

Conclusions and Current and Future Challenges
This paper presents the results of a review on emerging waterborne pathogens in Africa and specifically on Cryptosporidium. We reviewed 68 papers from indexed journals. The main results of the study are as follows. Several pathogenic microorganisms in Africa are likely to be threats to public health, in particular Cryptosporidium. Risk factors related to the consequences of climate change; social and behavioral factors of local populations; water problems; geographical positions that can cause isolation; and inequalities caused by the lack of transparency of African states in the distribution of financial resources are current and future challenges that African governments should address.
Although cryptosporidiosis is a major source of diarrheal disease worldwide, particularly in Africa, there is no effective treatment for it due to a perceived limited market. The sequencing of the genomes of C. parvum and C. hominis are advances that could identify new molecular targets for drug development. Due to the lack of treatment, especially for Cryptosporidiosis, high-level community awareness, policy formulation, and regular surveillance are essential factors in limiting the waterborne, zoonotic, and anthroponotic spread of Cryptosporidiosis [22,81]. These actions can only be taken if African leaders commit to providing communities with safe drinking water, improving sanitation by connecting people to sewers, and improving wastewater treatment. Community awareness programs on water safety, personal hygiene, and water treatment should be implemented. The achievement of these goals is conditional on the fight against malnutrition and a significant reduction in HIV rates in African populations. Unfortunately, the majority of African governments have not invested sufficient funds and resources to ensure that malnutrition and HIV are addressed and many HIV prevention services still do not reach most of the people who need them, largely due to staff shortages and the poor geographical distri-bution of existing services to those who need them most [82,83]. Despite the Millennium Development Goal of halving hunger by 2030, major failures remain in Africa. African authorities, on the other hand, have an interest in recognizing the future risks of waterborne protozoan diseases and responding quickly and effectively, because water-related crises will occur in Africa when African leaders do not have the skills and infrastructure to deal with them. As a result, African populations will use alternative water sources without adequate treatment or migrate to other countries. Disease and malnutrition will increase and waterborne protozoa will multiply. If the right decisions are not taken, Africa is likely to face more complex situations in the future. Only then can the catastrophic effects of waterborne diseases and their future consequences be mitigated [84]. Establishing a surveillance mechanism would be one of the first steps to control protozoan infections and improve community health in developing countries [48]. It is also recommended to initiate international collaboration against waterborne protozoan pathogens and to define a standardized reporting scheme. Seasonal patterns and distributions of different pathogens in Africa should also be assessed. This is particularly important given that adverse climatic events resulting from climate change are occurring more regularly on a global scale, which may lead to large outbreaks of waterborne infectious diseases [47]. Determining the molecular characteristics of Cryptosporidium should also be one of the priorities as molecular methods allow genetic determination of parasites isolated from water which can help determine the source of contamination. Drug development is essential for children suffering from diarrhea and malnutrition in Africa, as existing drugs against Cryptosporidium are mostly ineffective due to a poorly understood host-parasite interface [81,83].