Floating Wetland Islands Implementation and Biodiversity Assessment in a Port Marina

Floating wetland islands (FWI) are considered nature-based solutions with great potential to promote several ecosystem services, such as biodiversity and water quality enhancement through phytoremediation processes. To our knowledge, the present work is the first to scientifically document the in-situ establishment of an FWI in a seawater port marina. The establishment and performance of a cork floating platform with a polyculture (Sarcocornia perennis, Juncus maritimus, Phragmites australis, Halimione portulacoides, Spartina maritima, Limonium vulgare) was evaluated. The diversity of organisms present in the FWI was undertaken based on the macrofauna assessment, taking into consideration marine water characterization, with a focus on hydrocarbons. Microbial communities were assessed based on metabarcoding approach to study 16S rRNA gene from environmental DNA retrieved from biofilm (from the planting media), marine biofouling (from the submerged platform) and surface marina water. S. perennis was the species with the highest survival rate and growth. The structure of the microbial community showed clear differences between those established in the FWI and those in the surrounding water, showing the presence of some bacterial groups that can be relevant for bioremediation processes (e.g., Saprospiraceae family). Concerning the macrofauna analysis, Mytilus sp. was the predominant taxa. To be of relevance, total petroleum hydrocarbons were detected at the marina up to ca. 6 mg/L. This study gives new insights into broadening FWI application to the saline environments of port marinas and to supporting a management strategy to promote several ecosystem services such biodiversity, species habitat, water quality enhancement and added aesthetic value to the marina landscape.


Introduction
Coastal zones-such as those in Europe, Asia, Australia, and USA-are suffering urban expansion to an extent that more than 50% of the shoreline is transformed by engineering. Effects of the marine urbanization in relation to regional ecological consequences, connectivity changes, and water quality still lack comprehensive understanding. Marinas and harbors or ports are among the man-made structures that support maritime activities [1]. These sites may be characterized based on their location, structure, capacity, hydrology, and associated activities. Consequently, different anthropogenic pressures occur such as diesel spills during vessel refueling, hydrocarbon pollution from boat traffic, Floating wetland island location.

FWI Implementation and Maintenance
The pilot FWI was implemented on February 2018 and was under study for 16 months. The FWI system had three modules which were interconnected (Figure 2A). Each module (Cork Floating Island ® , Supplier: Bluemater, S.A., Porto, Portugal) had the following technical characteristics: cork agglomerate (density of 0.2), frustoconical holes of two different sizes (8 cm and 16 cm diameter for the small and the larger types, respectively), size of 100 × 50 × 10 cm (l × w × h), capacity to support until 16 kg/m 2 of plants and up to 24 plants/m 2 , according to the manufacturer [15].
The anchoring system was composed of two cables, each with a weight attached at the end, passed through a hole located in a corner of each side of the floating platform and tied to a metal structure on the top of the wall, allowing the FWI to accompany the tidal level fluctuations. Adjustable plastic clamps were used to reinforce the joints between the modules and between the holes and the vases ( Figure 2B,C). Plant species selected (see Section 2.3) were placed in coconut fiber vase, filled with rockwool ( Figure 2D). Vases were afterwards inserted into the holes of the platform in a random distribution of the plant species. During the implementation, each hole held the same planting density (eight seedlings of the same size for each species tested). All the holes were enumerated for further monitoring. Floating wetland island location.

FWI Implementation and Maintenance
The pilot FWI was implemented on February 2018 and was under study for 16 months. The FWI system had three modules which were interconnected (Figure 2A). Each module (Cork Floating Island ® , Supplier: Bluemater, S.A., Porto, Portugal) had the following technical characteristics: cork agglomerate (density of 0.2), frustoconical holes of two different sizes (8 cm and 16 cm diameter for the small and the larger types, respectively), size of 100 × 50 × 10 cm (l × w × h), capacity to support until 16 kg/m 2 of plants and up to 24 plants/m 2 , according to the manufacturer [15].
other side of the pier where the FWI was implemented. During the experiment, 91 cruise ships parked at the Porto Cruise Terminal (according to APDL: http://www.apdl.pt/cruzeiros/previsaochegada.jsp?lang.pt). The area of the Marina is also influenced by tides from the Atlantic Ocean and by the river mouth (Leça river) that flows through the Port. Floating wetland island location.

FWI Implementation and Maintenance
The pilot FWI was implemented on February 2018 and was under study for 16 months. The FWI system had three modules which were interconnected ( Figure 2A). Each module (Cork Floating Island ® , Supplier: Bluemater, S.A., Porto, Portugal) had the following technical characteristics: cork agglomerate (density of 0.2), frustoconical holes of two different sizes (8 cm and 16 cm diameter for the small and the larger types, respectively), size of 100 × 50 × 10 cm (l × w × h), capacity to support until 16 kg/m 2 of plants and up to 24 plants/m 2 , according to the manufacturer [15].
The anchoring system was composed of two cables, each with a weight attached at the end, passed through a hole located in a corner of each side of the floating platform and tied to a metal structure on the top of the wall, allowing the FWI to accompany the tidal level fluctuations. Adjustable plastic clamps were used to reinforce the joints between the modules and between the holes and the vases ( Figure 2B,C). Plant species selected (see Section 2.3) were placed in coconut fiber vase, filled with rockwool ( Figure 2D). Vases were afterwards inserted into the holes of the platform in a random distribution of the plant species. During the implementation, each hole held the same planting density (eight seedlings of the same size for each species tested). All the holes were enumerated for further monitoring. The anchoring system was composed of two cables, each with a weight attached at the end, passed through a hole located in a corner of each side of the floating platform and tied to a metal structure on the top of the wall, allowing the FWI to accompany the tidal level fluctuations. Adjustable plastic clamps were used to reinforce the joints between the modules and between the holes and the vases ( Figure 2B,C). Plant species selected (see Section 2.3) were placed in coconut fiber vase, filled with rockwool ( Figure 2D). Vases were afterwards inserted into the holes of the platform in a random distribution of the plant species.
During the implementation, each hole held the same planting density (eight seedlings of the same size for each species tested). All the holes were enumerated for further monitoring.
Maintenance aimed to minimize human intervention, with minimum interference. Therefore, only a surface cleaning of detritus, mainly plastics, accumulated in the platform was periodically made.

Plant Selection, Planting, and Monitoring
For the process of plant selection, the following criteria, were used: (1) native to Portugal; (2) potential to survive in hydroponic mode with tolerance to variable salinity levels; (3) presence in the region of the experimental site; (4) perennial species and (5) possibility to form a polyculture.
Overall, six halophyte plants were selected: Juncus maritimus, Halimione portulacoides, Phragmites australis, Sarcocornia perennis, Limonium vulgare, and Spartina maritima. All plants were collected in the region nearby the marina, being watered with seawater during 24 h prior to their implementation in the FWI.
To monitor the health status of plants, an in-situ and non-destructive method was used to measure the chlorophyll fluorescence, based on pulse-amplitude modulated (PAM) measurements. Measurements were performed in four different plants of S. perennis, (three records for the same predefined plant) during July and September of 2018 and February and April of 2019 survey. Briefly, plants were placed in dark conditions for 25 min (covered by an opaque black cloth), after which a PAM chlorophyll fluorometry (Junior PAM-Heinz Walz GmbH, Germany), fitted with a 1.5 mm light guide plastic fiber and a blue led (445 nm) light source, was applied to the plant green tissues. The measurements were performed following the manufacturer's instructions, using the standard default settings for rapid light curve (RLC) determination. The basic fluorescence yield (Fo) value was recorded after applying a modulated light that was sufficiently low (<0.1 µmol/m 2 ·s) and the maximal fluorescence yield (Fm) was measured by applying a strong light pulse of 10,000 µmol/m 2 ·s for 0.8 s. The parameters attained were automatically calculated by the WinControl-3 software (version 3.29; Walz) to quantify the characteristic parameters: maximum electron transport rate (ETRm) that reflects the photosynthetic capacity at light saturation conditions [16]; quantum efficiency of photosynthesis (α) that estimates the efficiency of light-harvesting complexes [17] and the Fv/Fm ratio that is defined as the maximum photochemical quantum yield of photosystem II if all the available reaction centers were open (lower values are associated with fewer open reaction centers available) [18].
During the experimental time, evaluation of the apparent health status of all the plants species was also visually performed, registering plant survival. The survival rate associated to each plant species, by the end of the experiment, was calculated as follows (Equation (1) It was considered that a plant died when the whole aerial parts turned brown or the whole plant started to slowly decompose after two months of persistent status.
Visual observations of the macrofauna present in the FWI system were performed and registered along the experimental time.

Water Sampling Procedure and Physicochemical Analysis
Although the setup of the FWI was in February 2018, an acclimation period of two months was considered, and sampling started in April 2018, ending in May 2019. Sampling survey occurred monthly, in the last days of each month. In October 2018, a new plantation was carried out to reinforce the existing plants. Therefore, no sampling was undertaken in that month nor on the following two (November and December 2018), considering this time period as an acclimation period for the plants.
Physicochemical parameters of the surface marina water (temperature, salinity, pH, and conductivity) were measured near the FWI, using a portable multi-parameter (MU 6100 L pHenomenal ® , VWR). Air temperature and relative humidity were recorded with an OH503 logger (Greutor), and weather conditions were registered in every campaign.
Surface marina water samples (considered the first 20 cm below the water surface) were collected for further analysis. For nutrient determination (ammonia, nitrite, and phosphate ions) samples were filtrated through a Whatman TM glass microfiber filter with a 1.2 µm pore size (GE Healthcare Life Sciences, UK) and analyzed following published optimized methodologies [19]. Nitrate ion was quantified by an adaptation of the spongy cadmium reduction technique [20]. Chemical oxygen demand (COD), used to estimate the amount of organic matter, was measured using Kits HI93754A-25 and HI93754B-25, LR: 0-150 mg/L and MR: 0-1500 mg/L, respectively, from Hanna Instruments (Limena, Italy). Total petroleum hydrocarbons (TPHs) and polycyclic aromatic hydrocarbons (PAHs) were determined according to previously optimized methods [21,22]. Selected PAHs were those considered by the United States Environmental Protection Agency as priority pollutants [23]: naphthalene (Nap), acenaphthylene (Acy), acenaphthene (Ace), fluorene (  Biofilm from the planting media (mainly rockwool), around plant roots, was collected with sterilized forceps from each plant species. Marine biofouling developed in the floating platform (non-planted holes and under surface) was collected by scratching the surface with a sterilized bistoury blade. For each matrix (biofouling and biofilm) four subsamples (from the planting media and floating platform) were pooled together to form a composite sample. Surface marina seawater sample was additionally collected in a 5 L plastic bottle for microbial diversity assessment. Once in the laboratory, samples of biofilm and biofouling were preserved at −80 • C for DNA extraction. The 5 L of collected seawater were filtered through two Sterivex TM (SVGV010RS, Merck Millipore, Portugal) filter with a 0.22 µm pore size with the assistance of a peristaltic pump; the two replicate filters per sample were sealed and stored at −80 • C for later environmental DNA extraction.

DNA Extraction, Quantification, and Sequencing
Environmental DNA (eDNA) was extracted from the FWI samples (biofilm from the planting media and biofouling from the floating platform) using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) and from water samples (Sterivex filters) using the DNeasy PowerWater Sterivex Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. Concentration and quality of eDNA were measured by fluorometry using the Qubit fluorometric quantitation kit (Qubit dsDNA High Sensibility Assay Kit, Thermo Fisher Scientific Inc., Waltham, MA, USA). The extracted eDNA was then used for 16S rRNA gene sequencing and metabarcoding analysis targeting prokaryotic communities. The sequencing method includes the amplification of the 16S rRNA gene fragment (hypervariable V4-V5 region; ≈412 bp) using the forward primer 515YF (5 -GTGYCAGCMGCCGCGGTAA-3 ) and reverse primer Y926R (5 -CCGYCAATTYMTTTRAGTTT-3 ) [24] and the paired-end sequencing was carried out by an Illumina MiSeq ® platform with the V3 chemistry, according to manufacturer's instructions (Illumina, Inc., San Diego, CA, United States) at Genoinseq facilities (Cantanhede, Portugal). Raw reads were extracted from Illumina MiSeq ® System in fastq format and quality-filtered with PRINSEQ version 0.20.4 [25] to remove sequencing adapters, reads with less than 100 bases and trim bases with an average quality lower than Q25 in a window of five bases. The forward and reverse reads were merged by overlapping paired-end reads with Adapter Removal version 2.1.5 [26] using default parameters.

Bioinformatics Analysis
Merged reads were further uploaded and processed by the automatic pipeline SILVAngs (Silva Next Generation Sequencing, https://ngs.arb-silva.de/silvangs/) of the SILVA rRNA gene database project (SILVAngs 1.3) for the taxonomic classification [27] using default settings. Specifically, each read was aligned using the SILVA Incremental Aligner (SINA v1.2.10 for ARB SVN (revision 21008)) against the SILVA SSU rRNA SEED and quality checked using pipeline s default setting. After quality control steps, reads were dereplicated and clustered at 98% of similarity to generate operational taxonomic units (OTUs); the classification was done against SILVA SSU Ref dataset release 132 using blastn (2.2.30+; http://blast.ncbi.nlm.nih.gov/Blast.cgi) with standard settings [28]. Reads without any BLAST hits or reads with weak BLAST hits, remained unclassified and were labeled as 'no relative'. Protocol detailed description is given in Bragança et al. [29].
Abundance tables at different taxonomic levels: higher (phylum) and lower level (genus) have been produced to show the relative abundance of each taxonomic group within each sample (the total number of sequences assigned to each taxonomic path). Undesirable lineages, 'chloroplast', 'mitochondria', and 'eukaryota' were excluded from the dataset.

Statistical Analyses
The statistical analysis was performed using PAST software (version 3.24; https://folk.uio.no/ ohammer/past/) to analyze data at a 95% confidence level and significance between treatments was considered as statistically significant if the p-value <0.05. All the data subjected to statistical analyses were examined with Shapiro-Wilk test to confirm the existence of normal distribution and an evaluation of the homogeneity of variance assumption was performed by applying Levene's test. When data presented normal distribution, a one-way analysis of variance (ANOVA) was performed and when statistically significant differences for ANOVA were found, a Tukey post-hoc test was executed. All data were expressed as mean ± standard deviation (SD). The alphabet letters labeled over the values represent the statistically significant/non-significant differences between or within treatments.

FWI Establishment and Macrofauna Monitoring
The FWI platform chosen for this study was made of cork, a natural material, with a negative carbon footprint considering that the cork sector is a net carbon sink, since along the entire life cycle more carbon is sequestered than emitted, having thus the potential to mitigate climate change [30]. This FWI preserved its structural integrity, along the studied period, when subject to the dynamics of the seawater port marina, facing various storms, tides, waves, currents, and other abiotic deteriorating factors such as salinity and direct sunlight ( Figure 3).
Over time, successive colonization was observed on the floating platform by marine macrofauna (Figure 4). Initially, the first organisms colonizing the FWI (under surface) were members from the Polychaeta class and Chthamalus genus. At the end of this study, the floating platform was covered mainly by Mytilus sp. (under surface and holes) and by a variety of macroalgaes (on the boundaries and holes). The high proliferation of Mytilus sp. under FWI surface increased the weight of the platform and prevented the roots to develop through the water column. However, plant development did not seem to be affected since the planting media remained wet and they continued to develop. In the future, this may constitute a problem if the accumulated weight compromises the island s buoyancy.
In a similar application at Baltimore Inner Harbor, the same issue related to biofouling in an FWI was reported by Mccarty et al. [13].
FWI platform chosen for this study was made of cork, a natural material, with a n ootprint considering that the cork sector is a net carbon sink, since along the entire li rbon is sequestered than emitted, having thus the potential to mitigate climate chan I preserved its structural integrity, along the studied period, when subject to the dy eawater port marina, facing various storms, tides, waves, currents, and other ting factors such as salinity and direct sunlight ( Figure 3).  Over time, successive colonization was observed on the floating platform by marine macrofauna (Figure 4). Initially, the first organisms colonizing the FWI (under surface) were members from the Polychaeta class and Chthamalus genus. At the end of this study, the floating platform was covered mainly by Mytilus sp. (under surface and holes) and by a variety of macroalgaes (on the boundaries and holes). The high proliferation of Mytilus sp. under FWI surface increased the weight of the platform and prevented the roots to develop through the water column. However, plant development did not seem to be affected since the planting media remained wet and they continued to develop. In the future, this may constitute a problem if the accumulated weight compromises the island′s buoyancy. In a similar application at Baltimore Inner Harbor, the same issue related to biofouling in an FWI was reported by Mccarty et al. [13]. The upper surface of the platform was, in general, dry (with exception of periodic flooding due to waves from the tides), receiving total light exposure, which may explain the lower colonization. However, over time, with the FWI sinking partially (due to the overall weight of the platform), this part received more water and organisms such as Ectocarpus sp. and Ulva sp. installed in a major proportion in that area. This was registered after 12 months of FWI implementation. Considering mobile organisms, Palaemon sp. was observed actively interacting with the detritus present in the non-planted holes and Mugil sp. was observed in the water body pecking below the FWI platform and in the surrounding water, possibly for feeding interest. In the same port where the FWI were set, Azevedo et al. [31] carried out a study focusing on the biofouling diversity development on stainless steel plates using a combined morphological and metabarcoding approach. They have identified several common colonizers similar to the ones found in the FWI, such as mussels, barnacles, and macroalgae. Other authors also mentioned the presence of several organisms in FWI implemented in freshwater ecosystems [32,33]. The biodiversity increment observed in the present study might be advantageous because some of these organisms, such as Ulva genus members, have the capacity to remove several pollutants [34], and mussels are ecosystem engineers, with capacity to form The upper surface of the platform was, in general, dry (with exception of periodic flooding due to waves from the tides), receiving total light exposure, which may explain the lower colonization. However, over time, with the FWI sinking partially (due to the overall weight of the platform), this part received more water and organisms such as Ectocarpus sp. and Ulva sp. installed in a major proportion in that area. This was registered after 12 months of FWI implementation. Considering mobile organisms, Palaemon sp. was observed actively interacting with the detritus present in the non-planted holes and Mugil sp. was observed in the water body pecking below the FWI platform and in the surrounding water, possibly for feeding interest. In the same port where the FWI were set, Azevedo et al. [31] carried out a study focusing on the biofouling diversity development on stainless steel plates using a combined morphological and metabarcoding approach. They have identified several common colonizers similar to the ones found in the FWI, such as mussels, barnacles, and macroalgae. Other authors also mentioned the presence of several organisms in FWI implemented in freshwater ecosystems [32,33]. The biodiversity increment observed in the present study might be advantageous because some of these organisms, such as Ulva genus members, have the capacity to remove several pollutants [34], and mussels are ecosystem engineers, with capacity to form biodiversity enhancing reef-like structures [35].

Evaluation of Plant Establishment and Development
One of the preliminary objectives was to evaluate which plant species would survive and establish under the tested conditions. For that, the plants were collected from an estuarine region nearby the port, having in consideration similar salinity levels [36]. From the six FWI plants initially planted, only two species survived: S. maritima and S. perennis. S. maritima exhibited resilience (67% of survival), although, the optimal growth for this species seems to occur at 20% of seawater [37]. Overall, S. perennis demonstrated the greatest potential among the other six plant species tested, presenting survival rates up to 88%. This marsh plant evidenced visual growth in such a way that it began to spread to the other holes of the FWI, particularly after 16 months of the implementation. This propagation tendency is characteristic of this perennial succulent species, with the subshrubs tending to merge, forming agglomerates and creating mats of 1 m or more in diameter [38]. The other plant species decayed rapidly, no specimens alive remaining after November 2018. Nevertheless, further studies with J. maritimus, H. portulacoides, P. australis, and L. vulgare would be of interest, to understand their low success of establishment, for instance, if it is related with the conditions of the marina, the sampling season or plant physiologic characteristics. Thus, the two more resistant and more promising plant species to cope with this environment, S. maritima and S. perennis, seem to be the most suitable species for future FWI applications in highly saline water bodies. Sanicola et al. [14] reported that a plant from the same genus (Sarcocornia quinqueflora) was the one with the better root biomass growth in saltwater (rather than in freshwater) among the other four wetland plant species tested in their FWI mesocosm studies. Moreover, Calheiros et al. [39] used Sarcocornia fruticosa for highly saline wastewater treatment, in a constructed wetland, where this species also demonstrated a high resilience.
PAM measurements were performed in S. perennis, which early showed the most promising survival rate ( Figure 5). Results showed the highest and significantly different ETRm value in April of 2019, with high values for the other two parameters registered as well. On the other hand, in February of 2019 the lowest values of all the three parameters tested were registered, including significantly different Fv/Fm ratio and quantum efficiency of photosynthesis.
These results suggested a better photosynthetic performance in the months of higher temperature and higher number of daily light hours (Summer and Spring), considering that in the first PAM measurement (five months after the FWI implementation, July 2018), plants had already adapted to the environmental conditions. Thus, these results indicated the absence of major concerns, considering the photosynthetic performance. Nevertheless, no measurements were found, in the literature, for similar environmental conditions and plants species, to establish a direct comparison. a high resilience.
PAM measurements were performed in S. perennis, which early showed the most promising survival rate ( Figure 5). Results showed the highest and significantly different ETRm value in April of 2019, with high values for the other two parameters registered as well. On the other hand, in February of 2019 the lowest values of all the three parameters tested were registered, including significantly different Fv/Fm ratio and quantum efficiency of photosynthesis. These results suggested a better photosynthetic performance in the months of higher temperature and higher number of daily light hours (Summer and Spring), considering that in the first PAM measurement (five months after the FWI implementation, July 2018), plants had already adapted to the environmental conditions. Thus, these results indicated the absence of major concerns, considering the photosynthetic performance. Nevertheless, no measurements were found, in the literature, for similar environmental conditions and plants species, to establish a direct comparison.

Seawater Port Marina Characterization
To have a characterization of the water body where the FWI were implemented, several physicochemical parameters were measured over time (Table 1). At each monthly sampling campaign, the air temperature and relative humidity ranged from 13.1 • C to 20.7 • C and from 26% to 71%, respectively.
Considering salinity and conductivity values, the water of the marina can be classified as highly saline [40]. COD values showed high variability over time, ranging between 135 and 630 mg O 2 /L in the marina water. Nutrient concentrations of the four inorganic ions measured were higher than the ones found in the coastal areas along the northwest Portuguese coast [41] and lower than those reported in a study monitoring coastal areas of the same region and relatively close to the present study area [42]. In the case of the marina, the waters are more confined, with lower hydrodynamic than coastal waters, and with the direct influence of the Leça river, as the marina is located on the river mouth. These facts may explain the nutrient concentrations and high COD values observed in the present study. Regarding hydrocarbon levels, TPHs concentrations were often below the detection limit (<2 mg/L) but were also detected at a level of ca. 6 mg/L. This concentration was higher than the ones found in coastal areas influenced by shipping activities or near oil explorations of South Africa [43], Iran [44], Thailand [45], and China [46], where levels detected were up to 0.51 mg/L. The TPH methodology used gives mostly an estimation of total aliphatic hydrocarbons concentration which are generally the hydrocarbons more easily degraded. Therefore, their presence might indicate a continuous source of hydrocarbons. A more extensive survey of the marina water should be considered to evaluate in detail the presence of these hydrocarbons and possible relations with the traffic of boats, although this marina has been working at a low traffic intensity. Concerning PAHs, concentrations were below the detection limit in all water samples collected (detection limit: Nap < 1 ng/L; Acy < 8.6 ng/L; Ace  [42] detected PAHs in the water near by the present study area (between 48.5 and 60.3 ng/L), indicating that PAHs sources were both pyrogenic and petrogenic. A possible explanation for the detection of TPHs and no PAHs in the present study, can be related to the tendency of the latest for deposition and accumulation on sediment bed [47,48]. Since marinas are relatively confined areas, they can accumulate more fine sediments with associated organic matter which can adsorb these organic pollutants [47]. The water characteristics may have influenced the plant survival and growth to some extent, with the high salinity and availability of nutrients in the marina water body in consideration, because halophytes species differ greatly in terms of their salt tolerance [49]. The nutrients availability can be a restrictive factor for plant growth in saline habitats [50].

Assessment of Microbial Diversity in the FWI and Water
FWI samples of biofouling and biofilm were analyzed. The accumulation of organisms on submerged structures (e.g., pier), in marine environments context, often receives the designation of biofouling. However, this multi-stage process starts with biofilm formation [51]. Biofilm designation, in the context of FWI, is often attributed to the microbial development associated to the plant roots and its role on the water depurative process [7,52]. Therefore, both types of samples were considered. Water microbial diversity was also assessed.
A total of 764,759 of 16S rRNA gene sequences were generated by Illumina MiSeq sequencing for all the samples processed by SILVAngs analysis pipeline, which decreased to a total of 606,549 (79.31%) after quality filtering. From those, 593,753 (77.64%) were classified and 12,796 (1.67%) remained classified as 'no relative' reads (without any close relatives).
The analysis of alpha-diversity indices across all the samples ( Figure 6A) showed a pattern within each type of samples in relation with the observed number of genera (ONG), with an increase from the Summer of 2018 to the Winter of 2019, followed by a decrease in the Spring of 2019 but with values still higher than the Summer of 2018, showing a possible seasonality. The lower value of ONG in Summer 2018 can be related to more intense maritime traffic that occurred in the study area. A previous study by Nogales and Bosch [2] mentioned shifts in the bacterial composition due to the impact of the activities of recreational coastal areas. However, other factors must be considered such as the natural colonization process, and the possible seasonality referred. Antunes et al. [53] also suggested that pioneer bacterial biofilm communities may face significant seasonal variations in the same location, when carrying out a study in the same port of the present study. They have stated that samples of biofilm retrieved from two different seasons had shown significant distinct taxonomic profiles and diversity patterns of the bacterial communities.  ). This top 20 also includes one taxon from the Flavobacteriales order and one taxon of Gammaproteobacteria class (unculture representative). Antunes et al. [53] when studying the biofilm growing on stainless steel surfaces in the same port, reported that the most abundant family found was Rhodobacteraceae, followed by Flavobacteriaceae, Halomonodaceae, and Alteromonodaceae families. The Shannon diversity index remained stable over time for each type of sample. However, the highest values were detected for the biofilm from the planting media of S. perennis, presenting slight differences in comparison to the other plant species. Oliveira et al. [54] also reported a higher Shannon index in bacterial communities of S. perennis (subsp. perennis) rhizosphere comparing to H. portucaloides rhizosphere in natural sediments affected by hydrocarbons. When the same type of samples (biofouling from the floating platform, biofilm from the planting media, and water) was grouped ( Figure 6B) and compared, no significant ONG differences were detected between groups. Shannon diversity varied significantly for the samples of biofilm from the planting media compared to the water samples and, although not significantly different, this index was also higher compared to the samples of biofouling from the floating platform. Figure 7 shows the top 20 taxa across the dataset, which includes the genera from seven different families and the 'no relative' group: Flavobacteriaceae (Aurantivirga sp., uncultured representative, Maribacter sp., Winogradskyella sp., Psychroserpens sp., Maritimimonas sp.), Rhodobacteraceae (uncultured representative, Loktanella sp., Sulfitobacter sp., Amylibacter sp., Lentibacter sp.), Saprospiraceae (uncultured representative, Lewinella sp.) Alteromonadaceae (Glaciecola sp.), Halieaceae (Halioglobus sp.), Cryomorphaceae (uncultured representative), Gottschalkiaceae (Gottschalkia sp.). This top 20 also includes one taxon from the Flavobacteriales order and one taxon of Gammaproteobacteria class (unculture representative). Antunes et al. [53] when studying the biofilm growing on stainless steel surfaces in the same port, reported that the most abundant family found was Rhodobacteraceae, followed by Flavobacteriaceae, Halomonodaceae, and Alteromonodaceae families. The average relative abundance of the main phyla for each type of samples (biofouling from the floating platform, biofilm from the planting media and water), is displayed in Figure 8, being the most abundant phyla Proteobacteria and Bacteroidetes (Appendix A Figure A1 shows the top 10 phyla found per sample analyzed). Cyanobacteria and Planctomycetes were also relevant phyla on biofouling and biofilm samples. Studies on biofouling growth on stainless steel plates in the same port marina as the present study, showed that the most abundant phyla were Cyanobacteria, Proteobacteria, Bacteroidetes, and Fusobacteria [31] as well as Proteobacteria, Bacteroidetes, Cyanobacteria, and Actinobacteria [53]. The beta-diversity analyzed through the principal coordinates analysis (PCoA) (Figure 9) showed a clear distinct communities' composition developed in the biofouling and biofilm compared with the water. These differences were expected as the distinctness between water and marine biofilm The average relative abundance of the main phyla for each type of samples (biofouling from the floating platform, biofilm from the planting media and water), is displayed in Figure 8, being the most abundant phyla Proteobacteria and Bacteroidetes (Appendix A Figure A1 shows the top 10 phyla found per sample analyzed). Cyanobacteria and Planctomycetes were also relevant phyla on biofouling and biofilm samples. Studies on biofouling growth on stainless steel plates in the same port marina as the present study, showed that the most abundant phyla were Cyanobacteria, Proteobacteria, Bacteroidetes, and Fusobacteria [31] as well as Proteobacteria, Bacteroidetes, Cyanobacteria, and Actinobacteria [53]. The average relative abundance of the main phyla for each type of samples (biofouling from the floating platform, biofilm from the planting media and water), is displayed in Figure 8, being the most abundant phyla Proteobacteria and Bacteroidetes (Appendix A Figure A1 shows the top 10 phyla found per sample analyzed). Cyanobacteria and Planctomycetes were also relevant phyla on biofouling and biofilm samples. Studies on biofouling growth on stainless steel plates in the same port marina as the present study, showed that the most abundant phyla were Cyanobacteria, Proteobacteria, Bacteroidetes, and Fusobacteria [31] as well as Proteobacteria, Bacteroidetes, Cyanobacteria, and Actinobacteria [53]. The beta-diversity analyzed through the principal coordinates analysis (PCoA) (Figure 9) showed a clear distinct communities' composition developed in the biofouling and biofilm compared with the water. These differences were expected as the distinctness between water and marine biofilm communities have been already reported by other authors [55,56]. Based on these results, ANOSIM The beta-diversity analyzed through the principal coordinates analysis (PCoA) (Figure 9) showed a clear distinct communities' composition developed in the biofouling and biofilm compared with the water. These differences were expected as the distinctness between water and marine biofilm communities have been already reported by other authors [55,56]. Based on these results, ANOSIM confirmed, statistically, a significant difference (R 2 = 1, p = 0.012) between the water samples and the biofilm samples from the planting media as well between the water samples and the biofouling samples from the floating platform (R 2 = 1, p = 0.027). On the other hand, ANOSIM between the biofouling samples from the floating platform and the biofilm samples from the planting media showed no significant difference (R 2 = 0.28, p = 0.073).  Additionally, SIMPER analysis was performed to identify the taxa (at genus level) that most contributed to the differences between the two types of samples in comparison to the water samples (Appendix A Tables A1 and A2). This analysis showed a higher relative abundance of Maritimimonas sp., Gottschalkia sp., Maribacter sp., and Lewinella sp. Results showed also a higher relative abundance of uncultured Saprospiraceae and Gammaproteobacteria classes in the case of the biofilm samples from the planting media. Previous studies reported the presence of the Maritimimonas genus, in high percentage, in biofilms developed on biocidal coatings [57] and plastic surfaces at harbor environments [58], suggesting a great resistance capacity. In the present study, this specie was found, mainly, in the under surface of the floating platform (Figure 7). The Gottschalkia genus is characterized by the ability to degrade purines. Interestingly, Gottschalkia genus members are classified as obligatory anaerobic [59], although, this genus was found in the floating platform (presenting aerobic conditions). Members affiliated to the Maribacter genus are, usually, found in association with macroalgae Ulva sp. [60,61]. Concerning Lewinella genus, this genus has been found in samples retrieved from marine environment [62]. With relation to the Saprospiraceae family, members are mostly isolated from marine environments (but also freshwater) and activated sludge, having shown capacity to breakdown complex organic compounds [63]. Therefore, this group of organisms can be important for bioremediation processes, being mainly present in the biofilm from the planting media in the present study. Finally, the Gammaproteobacteria class that contributed to the dissimilarity between water and the biofilm from the planting media, was previously found in other studies, in a high relative abundance associated with the rhizosphere of salt marsh plants as well [64]. Additionally, SIMPER analysis was performed to identify the taxa (at genus level) that most contributed to the differences between the two types of samples in comparison to the water samples (Appendix A Tables A1 and A2). This analysis showed a higher relative abundance of Maritimimonas sp., Gottschalkia sp., Maribacter sp., and Lewinella sp. Results showed also a higher relative abundance of uncultured Saprospiraceae and Gammaproteobacteria classes in the case of the biofilm samples from the planting media. Previous studies reported the presence of the Maritimimonas genus, in high percentage, in biofilms developed on biocidal coatings [57] and plastic surfaces at harbor environments [58], suggesting a great resistance capacity. In the present study, this specie was found, mainly, in the under surface of the floating platform (Figure 7). The Gottschalkia genus is characterized by the ability to degrade purines. Interestingly, Gottschalkia genus members are classified as obligatory anaerobic [59], although, this genus was found in the floating platform (presenting aerobic conditions). Members affiliated to the Maribacter genus are, usually, found in association with macroalgae Ulva sp. [60,61]. Concerning Lewinella genus, this genus has been found in samples retrieved from marine environment [62]. With relation to the Saprospiraceae family, members are mostly isolated from marine environments (but also freshwater) and activated sludge, having shown capacity to breakdown complex organic compounds [63]. Therefore, this group of organisms can be important for bioremediation processes, being mainly present in the biofilm from the planting media in the present study. Finally, the Gammaproteobacteria class that contributed to the dissimilarity between water and the biofilm from the planting media, was previously found in other studies, in a high relative abundance associated with the rhizosphere of salt marsh plants as well [64].

Conclusions
The establishment of FWI in a seawater port marina demonstrated promising results for the possible application in full scale of this NBS, promoting several ecosystem services such biodiversity, species habitat, aesthetic improvement of the landscape, and potential for water quality enhancement.
The characterization of the marina water constitutes an important dataset for future comparison with other harbors/marinas in Europe and in other locations, important due to the lack of data. The experimental floating platform, made of cork agglomerate, showed resistance to the environmental harsh conditions of the port marina, along the tested period. However, some adjustments for robustness improvement and buoyancy could improve the system and should be taken into consideration in future work. Also, a longer monitoring period of the FWI would be important to acquire more knowledge concerning the life span and resilience of the platform. This study showed that the halophyte plants Sarcocornia perennis and Spartina maritima can be used in future FWI seawater applications.
The characterization of the microbial community revealed the presence of some bacterial groups that can be relevant for bioremediation processes. Overall, the knowledge gained about the microorganisms and macrofauna diversity developed on the FWI will be important for future applications of FWI for water quality improvement and biodiversity promotion.
This study gives new insights to broaden the FWI application to the saline environment of port marinas and to support a management strategy that benefit from the ecosystem services that this NBS provides.