Effect of a Submerged Zone and Carbon Source on Nutrient and Metal Removal for Stormwater by Bioretention Cells

A bioretention system is a low-impact and sustainable treatment facility for treating urban stormwater runoff. To meet or maintain a consistently satisfactory performance, especially in terms of increasing nitrogen removal efficiency, the introduction of a submerged (anoxic) zone (SZ) combined with a module-based carbon source (C) has been recommended. This study investigated the removal of nitrogen (N), phosphorus (P) and heavy metals with a retrofitted bioretention system. A significant (p < 0.05) removal enhancement of N as well as total phosphorus (TP) was observed, in the mesocosms with additions of exogenous carbon as opposed to those without such condition. However, even in the mesocosm with SZ alone (without exogenous C), TP removal showed significant enhancement. With regard to the effects of SZ depth on nutrient removal, the results showed that the removal of both N and P in module with a shallow SZ (200 mm) showed significant enhancement compared to that in module with a deep SZ (300 mm). Removal efficiencies greater than 93% were observed for all three heavy metals tested (Cu, Pb, and Zn) in all mesocosms, even in the bioretention module without an SZ or plants, and it indicated that adsorption by the filtration media itself is probably the most important removal mechanism. Only Cu (but not Pb or Zn) showed significantly enhanced removal in module with an SZ as compared to those without an SZ. Carbon source played a minor role in metal removal as no significant (p > 0.05) improvement was observed in module with C as compared to that without C. Based on these results, the incorporation of SZ with C in stormwater biofilters is recommended.


Introduction
Land use change caused by urbanization can cause detrimental impacts on natural waters as a consequence of infiltration decreasing and runoff volumes increasing dramatically by high-density and impervious surfaces [1,2].Increased pollutant loads accumulated in surface runoff from impervious catchments without rational management discharged to the received water-bodies can make the quality of water irreversibly degraded [3,4].Bioretention cells (BCs) integrated runoff retention areas, is a critical component of Low Impact Development (LID) practices receiving high attention as "green storm infrastructure" to manage storm runoff in a decentralized and source management approach [5,6].BCs have gained popularity because of its design flexibility in terms of size and location, appealing landscape aesthetics, and hydrological performance for reduction of pollutants [7,8].Previous studies have demonstrated that BCs implemented in relatively small catchments effectively improve both water quantity and quality in response to frequent storm events [9][10][11].
In the vegetated filter media, pollutants from storm runoff can be removed through a variety of mechanisms, including physical, chemical and biological processes [12][13][14], and its quality is further enhanced by plant uptake and biological activities in the rhizosphere [15,16].In recent years, research has shown that BCs can effectively improve water quantity and remove suspended solids [17,18], nutrients [19][20][21], and heavy metals [22,23].However, it is difficult to meet or maintain a consistently satisfactory performance for reducing nitrogen due to a lack of effective denitrification [24,25].
Since it is difficult to achieve consistent high nitrogen removal in standard stormwater bioretention systems, a submerged (anoxic) zone (SZ) combined with a module of BCs added carbon source (C) (e.g., wood chips, shredded newspaper, sawdust etc.) has been used to improve the capacity for nitrogen removal by enhancing microbial denitrification [26][27][28].Zinger et al. [29] investigated the nitrogen transformation in biofilters through the design optimization by adding SZ and C, and reported that nitrate removal of up to 99% was achieved.Similarly, Kim et al. [27] evaluated different electron-donor substrate (e.g., alfalfa, leaf mulch compost, newspaper, sawdust, wood chips and wheat straw) for denitrification, and demonstrated that newspaper-supported biological denitrification was the most efficient method under conditions of intermittent loading, and the pilot-scale modified bioretention system showed the mass removal for nitrate and nitrite up to 80%.Zinger et al. [30] investigated the impact of drying/wetting cycling on biofilter performance, and revealed that the SZ was able to enhance biofilter nitration removal recovery and make it less dependent on drying/wetting.
However, controversial findings regarding to the role of a retrofitted SZ in the BCs were also observed in the previous studies [31].Dietz and Clausen [32] reported that in their field experiment that there was no sufficient evidence of improvement on a reduction of nitrate when integrated with an SZ in BCs.Similarly, the results from field experiments of both BCs conducted at a campus of Maryland University, one of which was designed to consist of an anoxic zone, showed no obvious difference in removal provided by both modules [33].Zinger et al. [28] revealed that TP removal was less efficient after retrofitting the SZ due to the presence of organic matter in the filter media with the SZ and the removal of metals was not affected in practical term, although NO x removal was significantly increased which enhanced overall N removal.Furthermore, the impact of SZ with carbon addition on metal removal has not been fully studied.It is of particular interest to ascertain whether the introduction of SZ and C will diminish the high metal removal achieved in standard bioretention design.Hence, it is still not clear whether the addition of SZ and C will significantly influence metal removal, since the trapping of the metals in the top layer of the soil plays a significant role in metal removal [34].In addition, to date, little information on the impact of the depth of SZ on contaminant removal in BCs is available.Also, there is relatively limited data available on water quality improvement (e.g., as a function of hydraulic residence time (HRT) and detailed pollutant fate in retrofited BCs with the saturated zone) [35,36].
Actually, BCs have been adopted by many urban catchments worldwide to reduce storm flows and improve runoff quality [37,38].In recent years, integrating the LID practices with the urban catchment for the sustainability of urban hydrology under the Sponge City Programme has been strongly implemented in China [39].Therefore, assessing the performance of LID practices including BCs is necessary for helping policymakers to determine the optimal plan for stormwater management, especially for the areas in China with rapid urbanization.In the present study, with great flexibility, the new modules designed can be implemented to fit easily into any site (e.g., car park, roadside planting verge, parks, small gardens or alongside a canal, etc.) as mesocosms (module systems).
In the event of drainage layer clogging, the new modules can be lifted out for necessary maintenance.In addition, the modular system enables vegetation to be planted before installation on site and therefore speeds up the establishment process and reduces the chances of soil erosion that could occur in the initial planting phase.Although research on the bioretention system with a retrofitted SZ and carbon source has been carried out in Australia [40,41] and USA [42,43], to date, few studies has been reported regarding the performance of modified BCs for matching the interests of Sponge City construction in China, and it is essential that further advancement of knowledge on the performance of BCs for improving runoff quality should be carried out.
This paper presents the results of a mesocosm scale study on the removal of nutrients (nitrogen and phosphorus) and heavy metals (copper, zinc, and lead) using BCs for three different designs storms.The main objectives of this study were to (1) evaluate the effects of introduction of SZ combined with C on the pollutant removal; (2) assess the influences of different SZ depths on the reduction of contaminants; and (3) investigate the relationship between HRT and contaminant removal.

Materials and Methods
Four mesocosms including (a) module without plants; (b) module with plants, as well as an SZ (depth of 300 mm) and C; (c) module with plants, as well as an SZ (depth of 200 mm) and C; and (d) module with plants as well as an SZ (depth of 200 mm), but without C, were set up at the campus of experimental plants, Shanghai, China.Figure 1 shows the layout of four bioretention systems.The dimensions of mesocosms were 1.20 m × 1.20 m × 1.20 m (length × width × depth).The mesocosms were housed under a transparent roof with open mesh on the sides, to ensure that only inflow water (rather than rainfall) was received during wet weather.
Water 2018, 10, x FOR PEER REVIEW 3 of 13 systems).In the event of drainage layer clogging, the new modules can be lifted out for necessary maintenance.In addition, the modular system enables vegetation to be planted before installation on site and therefore speeds up the establishment process and reduces the chances of soil erosion that could occur in the initial planting phase.Although research on the bioretention system with a retrofitted SZ and carbon source has been carried out in Australia [40,41] and USA [42,43], to date, few studies has been reported regarding the performance of modified BCs for matching the interests of Sponge City construction in China, and it is essential that further advancement of knowledge on the performance of BCs for improving runoff quality should be carried out.This paper presents the results of a mesocosm scale study on the removal of nutrients (nitrogen and phosphorus) and heavy metals (copper, zinc, and lead) using BCs for three different designs storms.The main objectives of this study were to (1) evaluate the effects of introduction of SZ combined with C on the pollutant removal; (2) assess the influences of different SZ depths on the reduction of contaminants; and (3) investigate the relationship between HRT and contaminant removal.

Materials and Methods
Four mesocosms including (a) module without plants; (b) module with plants, as well as an SZ (depth of 300 mm) and C; (c) module with plants, as well as an SZ (depth of 200 mm) and C; and (d) module with plants as well as an SZ (depth of 200 mm), but without C, were set up at the campus of experimental plants, Shanghai, China.Figure 1 shows the layout of four bioretention systems.The dimensions of mesocosms were 1.20 m × 1.20 m × 1.20 m (length × width × depth).The mesocosms were housed under a transparent roof with open mesh on the sides, to ensure that only inflow water (rather than rainfall) was received during wet weather.A prototype test run and seven column test runs were carried out to measure the permeability coefficient of modular retention media.The permeability of different soil depths was tested and the mean saturated hydraulic conductivity was 100 mm/h.The mesocosm in our study consisted of three layers.A description of the size, dimension, and property of the media in the mesocosm is as follows: (a) Filter layer (600-700 mm, top to down): (i) sandy loam (50%) (compost/washed sand ratio = 0.2:1) [31,32]; and (ii) medium to coarse sand (1-2 mm; D 60 (Particle size of the filter with a cumulative weight of 60%)= 2 mm) (50%); (b) Transition layer (50-100 mm): coarse sand (0.5-2 mm; D 60 = 1 mm); (c) Saturated zone (200-300 mm) with or without carbon source: (i) fine gravel (95%; 6-10 mm; D 60 = 8 mm); and (ii) with and without wood chips (5%).
Each mesocosm was planted with Hymenocallis speciosa.Hymenocallis speciosa was chosen because this species of plant can tolerate both flooding and dry weather conditions.There were 12 plants within a seedling stage in each tank.Irrigated with tap water, the plants were grown for 8 weeks in the mesocosms filled with filter media.Before the experiment began, the BCs were flushed four times for 2 weeks with artificial storm runoff to promote the development of natural biofilm.
For ensuring a stable inflow quality to BCs during the experiments, synthetic storm runoff was prepared by adding predetermined levels of chemicals into tap water, and was stored in plastic containers with the capacity of 150 L until use.The target chemical makeup of stormwater applied as synthetic runoff to bioretention system is presented in Table 1, largely in accordance with previous reported stormwater characteristics [45][46][47][48].Suspended solids (SS) were not included in the synthetic stormwater because of the potential for adsorption of the positively charged ammonium and metal ions at an unknown rate onto these particles during storage, and the absence of research standards relating to particulate size and ion-exchange properties.For this reason, the study referred to a number of published studies in omitting SS from their versions of synthetic stormwater [20,49].The synthetic storm runoff was continuously pumped to the top of the cells using a peristaltic pump at three different flow rate 11.2, 5.6 and 2.8 mL L −1 to reach HRT 1, 2 and 3 day, respectively.Effluent was also collected from the bottom of the cell every two days.The experiment cells in each HRT were continuously run two months.The experiment was operated from April to October 2016.
Effluent samples were collected from the underdrain pipe of each BC every 1, 2 and 3 day for different HRT in a 1-L amber glass bottle transported refrigerated to the laboratory and stored in a refrigerator at 4 • C until they were analyzed.The samples were immediately analyzed to investigate the concentration of the physicochemical parameters regarding temperature, dissolved oxygen (DO), pH value and conductivity measured using Multi-Parameter Digital Meter (HACH-HQ40d, Hach Company, Loveland, CO, USA) directly.In addition, ammonia-N (NH 4 + -N), nitrate (NO 3 − -N), and total phosphorus (TP), were analyzed using spectrophotometer (HACH-DR3800, Hach Company, Loveland, CO, USA) in accordance with the standard methods [50].Heavy metals were analyzed using nitric acid digestion followed by mass spectrometric analysis using an Elan DRC-e ICP-MS (Perkin Elmer, Waltham, MA, USA).Instrument detection limits were 0.5 µg L −1 for all the heavy metal tested.The monitoring results were tested for normal distribution.Investigating statistical differences between treatments were carried out by comparing the critical value through one-way analysis of variance.Comparisons were considered significantly different for p < 0.05 [51].

Physicochemical Parameters
Table 2 shows the results of the physicochemical parameters in the effluent of BCs.Only dissolved oxygen (DO) showed significant differences among the mesocosms, with the effluent level of DO in module (c) (with a 200 mm SZ and C) which was significantly lower (p < 0.05) than any of the other modules.Several studies have shown that DO (and redox potential) decrease in the SZ of BCs [52].Similarly in the present study, applying an SZ combined with C decreased the DO concentration in the effluent to the level of 4.55-6.08mg L −1 at three HRTs in module (c).In comparison, the DO concentration in the module (a) without SZ and C was in the rage of 7.07-8.55mg L −1 .Moreover, the effluent DO level in the module (b) (7.02-8.24mg L −1 ) was slightly higher than those in the module (d) (7.21-8.28mg L −1 ), indicating the impact of additional exogenous carbon.This finding is in agreement with the results reported by Blecken et al. [52] indicating both SZ and addition of C could decrease the DO concentration from around 8.5 to 6.0 mg L −1 .

Nitrogen Removal
The mean effluent concentrations of nitrogen (nitrate-N and ammonia-N) and phosphorus (dissolved and total P) for each BC are shown in Table 3. Poor NO 3 − removal in BCs has been reported by many researchers [24,53].High infiltration rates in BCs are likely to limit NO 3 − removal by denitrification [26].There are limited times for its biogeochemical processes and transformations.The effects of the additional exogenous carbon to drive denitrification could be clearly seen in BCs with the removal of nitrate (NO 3 − ) in module (c) with C showing significant (p < 0.05) enhancement (85%, 87% and 94%) at all three HRTs, respectively, compared to the removal efficiency by module (d) without C (74%, 76% and 82% at three HRTs, respectively) (Figure 2A).The fact that the addition of carbon source as electro donor can facilitate denitrification and improve NO 3 − removal in bioretention system has also been reported by other studies [38,54].The results also indicated that the removal of NO 3 − in module (c) with shallow SZ (200 mm) showed significant (p < 0.05) enhancement (85%, 87% and 94% at three HRTs, respectively) compared to those in the module (b) with deep SZ (300 mm) (71%, 73% and 86% at three HRTs, respectively).This might be due to the low DO concentrations in module (c) which facilitated the denitrification process.In general, the SZ can be characterized as a partly anoxic zone.However, given the relatively high DO concentrations in the tested BCs, in addition to denitrification, there must have been other removal mechanisms for NO 3 − removal, such as plant uptake.Lucas and Greenway [55] reported that nitrogen uptake seemed to exceed the amount of nitrogen retained in the treatments, suggesting that plants obtained additional nitrogen from the media and denitrification was not a major pathway involved in nitrogen proceedings.Moreover, compared to other modules, the poor NO 3 − removal performance in module (a) might be attributed to leaching of nitrate in unplanted systems when nitrification occurred during dry inter-event periods [56].
In addition, SZ resulted in improving the stability with less leaching for removal performance, and the addition C is also relevant with higher levels of nutrients and water for the plants [49].This concept of controlling redox conditions and organic content could therefore prove beneficial in areas where nitrate pollution is a major concern.The question remains, however, exactly why the mesocosms with the shallower submerged zone (200 mm as opposed to 300 mm) showed such significantly enhanced treatment performance.The leaching of nutrient from the compost-rich media and the immature state of the plants with weeks growing time resulting in the reduced root mass across the filter media profile, might be the main influential factors contributing to enhanced performance from the shallower system.HRT has been reported to be crucial for nitrogen removal in BCs in many studies [57][58][59][60].Figure 3 shows that the removal rate of nitrate was linearly related to HRT at the 1 to 3 day retention times to test, most likely reflecting first-order kinetics of denitrification in these reactors.The removal efficiencies of nitrate in each module increased with the increase in HRT.This finding is in good agreement with previous bioretention studies.Kim et al. [27] systematically evaluated a retrofitted bioretention system for nitrate removal via microbial denitrification, which incorporated a continuously SZ with an overdrain.The results from the nitrate loading and hydraulic loading studies in the second phase indicated that the mass of nitrate only, nitrate plus nitrite, and total nitrogen removed per day as a function of the volumetric nitrate loading.Lucas and Greenway [55] investigated P retention in bioretention mesocosms using media amended with different materials, and indicated that P retention by barren media eventually becomes exhausted under accelerated loading (24.5-29.3m year) used to simulate long-term exposure, even when the media is amended with P-sorbing materials.
The removal of ammonia (NH 4 + ) in module (c) with shallow SZ (200 mm) showed significant (p < 0.05) enhancement (78%, 87% and 91% at three HRTs, respectively) compared to those in module (b) with deep SZ (300 mm) (54%, 69% and 71% at three HRTs, respectively) (Figure 2B).Moreover, NH 4 + removal at all three HRTs in module (c) also showed significant (p < 0.05) enhancement compared to those in module (d), indicating that the depth of the SZ is an important design parameter which cannot be overlooked.Additionally, modules (b), (c) and (d) showed significantly (p < 0.05) enhanced NH 4 + removal at all three HRTs compared to those in module (a) with no SZ or plants (Figure 3).
Many researchers have reported that the removal of ammonia was mostly related to the adsorption on soil media during runoff dosing and nitrification during or between events [41].In our study, the better ammonium removal in module (c) (compared to module (b)) may be attributed to the thicker upper layer (with a corresponding greater contact surface and retention time for ammonium adsorption) and relatively shallow SZ (with a more oxygenated condition to stimulate nitrification).Additionally, the significant (p < 0.05) enhanced NH 4 + removal in module (c) (compared to module (d)) can be directly attributed to the addition of C, although the reason why exogenous carbon stimulates ammonia removal remains unclear.
to test, most likely reflecting first-order kinetics of denitrification in these reactors.The removal efficiencies of nitrate in each module increased with the increase in HRT.This finding is in good agreement with previous bioretention studies.Kim et al. [27] systematically evaluated a retrofitted bioretention system for nitrate removal via microbial denitrification, which incorporated a continuously SZ with an overdrain.The results from the nitrate loading and hydraulic loading studies in the second phase indicated that the mass of nitrate only, nitrate plus nitrite, and total nitrogen removed per day as a function of the volumetric nitrate loading.Lucas and Greenway [55] investigated P retention in bioretention mesocosms using media amended with different materials, and indicated that P retention by barren media eventually becomes exhausted under accelerated loading (24.5-29.3m year) used to simulate long-term exposure, even when the media is amended with P-sorbing materials.Table 3.The mean effluent concentrations of nitrogen and phosphorus (mg L −1 ) for bioretention mesocosms.

Phosphorous Removal
Many studies have found that the soil compartment is the major long-term phosphorus (P) storage pool and adsorption is the most important retention mechanism [60][61][62].Plants only contain a small amount of TP, thus TP uptake capacity of macrophytes is limited [56].Due to the minor role plant uptake plays in TP removal, module (a) was used as a control in the present study to examine the effect of a submerged zone on P removal.The removal efficiency in the mesocosm with the SZ alone showed significant (p < 0.05) enhancement in TP removal: TP removal efficiency in module (d) with SZ (42%, 45% and 49% at the three HRTs, respectively) was significantly enhanced, compared to module (a) without SZ (20%, 28% and 26% at three HRTs, respectively).This finding is consistent with a previous study conducted by Zhang et al. [54] who also reported that the removal for P significantly increased in the cell with SZ.Although the previous study by Lucas and Greenway [63] clearly showed that the presence of vegetation improved P removal, due to the immature plant development in the new cells given the short experimental period in the present study, we were unable to reconfirm the role of the plant in P removal.The poor TP removal performance in module (a) (sand filter alone) might best be attributed to a high level of leaching.Henderson et al. [51] reported that TP leaching was observed from a non-vegetated system when flushed with tap water under synthetic runoff applications, whereas leaching occurred in vegetated systems.In spite of this, in module (b) which has both plants and an SZ, TP removal efficiencies remained rather low (24%, 19% and 24% at three HRTs, respectively) (Figure 2C).These results point to the minimal role that vegetation plays in P removal in these BCs.As for the effects of SZ depth on TP removal, our results indicated that the removal of TP in module (c) with shallow SZ (200 mm) showed significant (p < 0.05) enhancement (72%, 71% and 74% at three HRTs, respectively) compared to those in the module (b) with deep SZ (300 mm) (24%, 19% and 24% at three HRTs, respectively).A previous study has demonstrated that the addition of carbon sources (e.g., methanol) has no effect on effluent P concentration in bioretention systems [41].Surprisingly, in the present study, given the same SZ depth, module (c) with C showed significant (p < 0.05) enhancement (72%, 71% and 74% at three HRTs, respectively) compared to the module (d) without C (42%, 45% and 49% at three HRTs, respectively).

Metal Removal
Copper (Cu), zinc (Zn) and lead (Pb) were selected for testing in the present study on heavy metal removal because there is robust literature on these metals in previous BCs research including both laboratory and field studies [19,64,65].At low concentrations, through their bioavailability and bioaccumulation in the aquatic environment, these pollutants may result in reduction of biodiversity and ultimately the contamination of human water and food supplies [66,67].Some of the heavy metals can have acute or chronic impacts on aquatic habitats, drinking water resources and recreational uses [68].In terms of potential toxic effects, the partitioning between the total and dissolved heavy metals is essential because the dissolved fractions are the most important ecotoxicants in stormwater and are directly biologically available [69].In particular, Zn and Cu have been shown to exhibit the highest intrinsic ecotoxicity in biomonitoring experiments using representative fish, algae, and macroinvertebrates, based on a number of runoff stages during rainfall events [70].Therefore, Zn deserves a high profile in research relating to urban stormwater contamination, whereas Cu might be a preferred indicator for atmospheric-mediated stormwater pollution, particularly where smelting of associated heavy metals occurs [20].
With influent levels of Cu, Zn, and Pb at 500, 1000 and 10 µg L −1 , removal efficiencies greater than 93% were observed in our BCs for each of the metals tested (Figure 4).Table 4 presents the mean effluent concentrations of heavy metals and removal efficiencies.This find is in good agreement with those of several other researches regarding that the effective performance of heavy metal removal from storm runoff (e.g., Cu, Zn, and Pb removal in excess of 90%).The filter media is the most important component affecting heavy metal removal [71].Sun and Davis [47] studied the capture of heavy metals in a bioretention system using three plant species with high biomass productivity, and reported that 88-97% of all inflowing metals were captured within the soil media, while the accumulation in plant tissue only accounted for 0.5-3.3% of the metals.Heavy metal removal has been shown to be high for a variety of different soil media, even in unvegetated soil filters, suggesting that the soil, rather than vegetation, plays the dominant role in metal removal [47,70].Since plants in biofilters have been shown to have a minor role [72], in our present study, module (a) without plants, served as an unplanted control to compare the removal efficiencies with other planted modules.In general, physical filtration also plays a role in contaminant removal, since heavy metals and some portion of P and N may attach to the sediment.For instance, Pb salts on road are mainly particle-bound.Thus, metal removal by bioretention may be an important strategy in avoiding accumulation of these substances in receiving water sediment.However, as the addition of suspended particulate matter to the simulated stormwater was not considered in this study the role of physical filtration seemed less important in the present study.Nevertheless, future field research on the metal removal by biofiltration and sedimentation is however needed.
served as an unplanted control to compare the removal efficiencies with other planted modules.In general, physical filtration also plays a role in contaminant removal, since heavy metals and some portion of P and N may attach to the sediment.For instance, Pb salts on road are mainly particlebound.Thus, metal removal by bioretention may be an important strategy in avoiding accumulation of these substances in receiving water sediment.However, as the addition of suspended particulate matter to the simulated stormwater was not considered in this study the role of physical filtration seemed less important in the present study.Nevertheless, future field research on the metal removal by biofiltration and sedimentation is however needed.The investigation of the effects of an SZ on metal removal performance showed that Cu removal ranged from 94 to 97% at all three HRTs in module (d) with an SZ, and was significantly (p < 0.05) enhanced compared to the removal efficiencies (92-94%) in module (a) without a SZ.For Pb, significant enhancement in module (c) compared to module (a) was also observed.In contrast, no significant (p > 0.05) enhancement was observed for Zn removal in module (c) with SZ.Higher heavy metal concentrations are observed after drought periods, and drying of soil is also associated with   The investigation of the effects of an SZ on metal removal performance showed that Cu removal ranged from 94 to 97% at all three HRTs in module (d) with an SZ, and was significantly (p < 0.05) enhanced compared to the removal efficiencies (92-94%) in module (a) without a SZ.For Pb, significant enhancement in module (c) compared to module (a) was also observed.In contrast, no significant (p > 0.05) enhancement was observed for Zn removal in module (c) with SZ.Higher heavy metal concentrations are observed after drought periods, and drying of soil is also associated with inactivity of biofilms and death of microorganisms and plant cells, which may result in flushing of a metal organic complex [73].However, SZ can provide a buffer to both soil and the plants against prolonged drying.Moreover, the creation of semi-anoxic zone through SZ is favorable for metal sorption to sediments [29], since heavy metals could be mobilized into the environment due to oxidation of sediments [74].
Additionally, the enhancement of heavy metal removal by the presence of SZ combined with C in BCs has been reported by numerous studies [28].The addition of C might improve complexation between copper and organic matter, due to solid organic matter absorbing Cu as the main component [75], while the other two metals possess lower affinity to organic matter [57].In order to investigate the role of carbon source in metal removal, metal removal by module (c) was compared with that of module (d).For Cu and Pb, no improvement was observed in module (c), compared with module (d), although a slight enhancement of Zn removal was observed in module (c) as compared to module (d).Our results are similar to other recent research by Blecken et al. [52] which demonstrated that the introduction of an SZ helps to enhance removal of Cu, but that no significantly better performance was observed for Pb or Zn.Even in the case of Cu, Blecken et al. [52] found that

Figure 1 .
Figure 1.Layout of four bioretention mesocosms (unit: mm).(a) module without plants or SZ; (b) module with plants, and a SZ (depth of 300 mm) with C; (c) module with plants, and a SZ (depth of 200 mm) with C; and (d) module with plants and a SZ (depth of 200 mm).These figures are referenced to FAWB (Facility for Advancing Water Biofiltration) Adoption Guidelines for Stormwater Biofiltration Systems [44].

Figure 1 .
Figure 1.Layout of four bioretention mesocosms (unit: mm).(a) module without plants or SZ; (b) module with plants, and a SZ (depth of 300 mm) with C; (c) module with plants, and a SZ (depth of 200 mm) with C; and (d) module with plants and a SZ (depth of 200 mm).These figures are referenced to FAWB (Facility for Advancing Water Biofiltration) Adoption Guidelines for Stormwater Biofiltration Systems [44].

Table 1 .
The characteristics of the synthetic storm runoff in this study.

Table 2 .
The physicochemical parameters in the effluent of bioretention mesocosms.
Note: n is number.

Table 3 .
The mean effluent concentrations of nitrogen and phosphorus (mg L −1 ) for bioretention mesocosms.