Modeling the Role of Wnt Signaling in Human and Drosophila Stem Cells

The discovery of induced pluripotent stem (iPS) cells, barely more than a decade ago, dramatically transformed the study of stem cells and introduced a completely new way to approach many human health concerns. Although advances have pushed the field forward, human application remains some years away, in part due to the need for an in-depth mechanistic understanding. The role of Wnts in stem cells predates the discovery of iPS cells with Wnts established as major pluripotency promoting factors. Most work to date has been done using mouse and tissue culture models and few attempts have been made in other model organisms, but the recent combination of clustered regularly interspaced short palindromic repeats (CRISPR) gene editing with iPS cell technology provides a perfect avenue for exploring iPS cells in model organisms. Drosophila is an ideal organism for such studies, but fly iPS cells have not yet been made. In this opinion article, we draw parallels between Wnt signaling in human and Drosophila stem cell systems, propose ways to obtain Drosophila iPS cells, and suggest ways to exploit the versatility of the Drosophila system for future stem cell studies.


Stem Cells in Human and Drosophila
Stem cells have the ability to self-renew and to produce specialized cells during development, normal organ function, and in response to tissue damage. Recent studies have shown the importance of the highly conserved Wnt signaling pathway in controlling the cell renewal process in various organisms ranging from humans to mice and flies [1,2]. The groundbreaking discovery that the expression of POU domain, class 5, transcription factor 1 (OCT4), sex determining region Y (SRY), transcription factor SOX-2 (SOX2), Kruppel-like factor 4 (KLF4), and myelocytomatosis oncogene (C-MYC) (also known as the Yamanaka factors) was able to induce pluripotency in differentiated cells meant that the seemingly unidirectional differentiation process could be reversed [3]. These factors have proven to be effective in human cells and mouse systems, but their application to Drosophila melanogaster, the ideal organism for rapid genetic studies, has not been explored adequately [4].

Wnt Signaling in Stem Cells
Wnt signaling refers to a collection of intracellular signal transduction pathways that result in a variety of cellular and developmental outcomes. First discovered as insertion sites for mouse mammary tumor virus [5] and patterning genes in Drosophila [6,7], the cloning of both fly and mouse Wnt1 showed just how well conserved signaling is and the close relationship between development, stem cells, and cancer [8][9][10]. The best studied of the Wnt pathways, or the so-called canonical signaling pathway, transduces the signal received from extracellular Wnt ligand binding by stabilizing the cytoplasmic With the destruction complex off, free β-Catenin translocates to the nucleus to transcribe target genes. When ESC were made with APC mutations that activated Wnt signaling by increasing β-Catenin levels, these cells were unable to differentiate into embryonic layers [45]. A similar result was observed in ES cells lacking both GSK3α and β isoforms [46].  LGR5, a Wnt target gene as well as Wnt signal enhancer, serves as a marker for stem cells. [17,27] Liver Mouse hepatocytes -Damage-induced Lgr5 + stem cells generate hepatocytes and bile ducts in vivo. [53] ESC: embryonic stem cell; EpiSC: epiblast-derived stem cell; LIF: leukemia inhibitory factor; DKK: dickkopf Wnt signaling pathway inhibitor; LGR5: leucine-rich repeat-containing G-protein coupled receptor 5; Wnt3a: wingless-type MMTV integration site family, member 3A.

Wnt Signaling and Stem Cells in Drosophila
In Drosophila, the major systems for addressing stem cell behavior have been germline stem cells and intestinal stem cells [54,55]. In the germline, the Wnt homologous gene wingless (wg) is required for stem cell maintenance. Disruption of canonical Wnt signaling in the inner germarial sheath cells (or escort cells) that surround the germline stem cells ( Figure 2) results in increased decapentaplegic (dpp) mRNA expression, expansion of germline stem-like cells, and increased bone morphogenetic protein (BMP) responsiveness in the germline ( Figure 2) [56,57]. Wnt signaling in the stem cell niche is increased with age in flies while BMP signaling is reduced, signifying the importance of Wnt signaling-mediated cell-cell communication to modulate niche stem cell signaling [57]. Another intriguing finding in the germline stem cell niche was that asymmetric cell division was regulated by Wnt components, suggesting a possible non-canonical signaling role in stem cells [58]. These non-canonical signaling pathways which influence apico-basal polarity have not been studied in great detail, especially as they relate to stem cells, but Drosophila stem cells and development show some possible avenues to explore [59][60][61]. The Drosophila model has also proven to be crucial in establishing how intestinal stem cells are maintained in their self-renewing stem cell state. Takashima et al. showed that the intestinal stem cells of the posterior intestine are localized to an anterior narrow region, and these are under the control of local Wnt1 and hedgehog (hh) signals [62]. Wnt proteins, like Hh proteins, are lipidmodified, which may constrain them to act as short-range signals [63]. This restricted expression of Wnt in the anterior region serves as a niche signal that maintains cells in a self-renewing mode. Moving away from the Wnt source, cells divide and move posteriorly as well as proliferate rapidly, during which hedgehog signaling takes over to allow cell cycle exit and differentiation. Finally, notch signaling results in differentiation into enterocyte cells (Figure 3) [62]. Additionally, recent work showed that adenoma-like structures form in response to Wnt activation in the fly intestine, recapitulating mammalian experiments [64,65], thus the Drosophila model provides insights into the spatial control and regulation of stem cell self-renewal. The Drosophila model has also proven to be crucial in establishing how intestinal stem cells are maintained in their self-renewing stem cell state. Takashima et al. showed that the intestinal stem cells of the posterior intestine are localized to an anterior narrow region, and these are under the control of local Wnt1 and hedgehog (hh) signals [62]. Wnt proteins, like Hh proteins, are lipid-modified, which may constrain them to act as short-range signals [63]. This restricted expression of Wnt in the anterior region serves as a niche signal that maintains cells in a self-renewing mode. Moving away from the Wnt source, cells divide and move posteriorly as well as proliferate rapidly, during which hedgehog signaling takes over to allow cell cycle exit and differentiation. Finally, notch signaling results in differentiation into enterocyte cells (Figure 3) [62]. Additionally, recent work showed that adenoma-like structures form in response to Wnt activation in the fly intestine, recapitulating mammalian experiments [64,65], thus the Drosophila model provides insights into the spatial control and regulation of stem cell self-renewal.

Benefits of Using Drosophila as a Model System
Drosophila melanogaster is a strong model organism for research into stem cell biology, possibly including pluripotency. Drosophila presents opportunities for powerful genetic manipulation; the ability to make straightforward, high-fidelity knock-outs, knock-ins, and knock-downs, allowing for great versatility in manipulating genes [66,67]. Additionally, modern imaging methods such as lightsheet microscopy and confocal microscopy allow for precise in vivo observation. These, when combined with optogenetic approaches perturbing signal transduction pathways, could translate into an in vivo 2i system, where pathways could be turned on or off and the effects could be imaged [68][69][70][71][72]. In mice, pluripotency factors have been demonstrated to be functionally redundant; therefore, given the relative difficulty of manipulating mice genetically, Drosophila may be an effective system to dissect the individual influences of each gene more carefully as related to Wnt signaling [38,73]. Transcriptional profiling approaches have shown some similarities between Wnt in human stem cells and fly cells, such as the gene Sp5 transcription factor (Sp5) [74][75][76].
Pluripotency genes have not yet been extensively characterized in Drosophila, although various populations of cells including the adult posterior midgut, embryonic nervous system, and germline have been found to develop from stem cell-like progenitors [77][78][79]. Recently, Rosello et al. demonstrated that mouse pluripotency factors could induce colony formation in Drosophila S2 cells as well as express a handful of endogenous Drosophila adult stem cell markers, indicating the ability to generate an iPS-like phenotype [4]. However, the ability of these iPS-like cells to form teratomas and chimeric embryos, rigorous tests of pluripotency in vivo, were not carried out. While the S2 cells did not become iPS cells, they attained many of the characteristics of iPS cells, indicating that these factors may have significant utility in the Drosophila system for investigating stem-cell and stem-celllike behavior. S2 cells are commonly used, but these experiments are fundamentally limited as S2 cells are secondary cell lines. Instead, primary cell lines and in vivo models should be more effective in producing results that are more relevant to organisms. Ideally, fly homologs of the Yamanaka factors should be used. Currently, the fly genes are hypothesized to be myc, luna, nubbin, and sox21a [80]. Once effective homologs are found which induce pluripotency, the fly system can be utilized as

Benefits of Using Drosophila as a Model System
Drosophila melanogaster is a strong model organism for research into stem cell biology, possibly including pluripotency. Drosophila presents opportunities for powerful genetic manipulation; the ability to make straightforward, high-fidelity knock-outs, knock-ins, and knock-downs, allowing for great versatility in manipulating genes [66,67]. Additionally, modern imaging methods such as light-sheet microscopy and confocal microscopy allow for precise in vivo observation. These, when combined with optogenetic approaches perturbing signal transduction pathways, could translate into an in vivo 2i system, where pathways could be turned on or off and the effects could be imaged [68][69][70][71][72]. In mice, pluripotency factors have been demonstrated to be functionally redundant; therefore, given the relative difficulty of manipulating mice genetically, Drosophila may be an effective system to dissect the individual influences of each gene more carefully as related to Wnt signaling [38,73]. Transcriptional profiling approaches have shown some similarities between Wnt in human stem cells and fly cells, such as the gene Sp5 transcription factor (Sp5) [74][75][76].
Pluripotency genes have not yet been extensively characterized in Drosophila, although various populations of cells including the adult posterior midgut, embryonic nervous system, and germline have been found to develop from stem cell-like progenitors [77][78][79]. Recently, Rosello et al. demonstrated that mouse pluripotency factors could induce colony formation in Drosophila S2 cells as well as express a handful of endogenous Drosophila adult stem cell markers, indicating the ability to generate an iPS-like phenotype [4]. However, the ability of these iPS-like cells to form teratomas and chimeric embryos, rigorous tests of pluripotency in vivo, were not carried out. While the S2 cells did not become iPS cells, they attained many of the characteristics of iPS cells, indicating that these factors may have significant utility in the Drosophila system for investigating stem-cell and stem-cell-like behavior. S2 cells are commonly used, but these experiments are fundamentally limited as S2 cells are secondary cell lines. Instead, primary cell lines and in vivo models should be more effective in producing results that are more relevant to organisms. Ideally, fly homologs of the Yamanaka factors should be used. Currently, the fly genes are hypothesized to be myc, luna, nubbin, and sox21a [80]. Once effective homologs are found which induce pluripotency, the fly system can be utilized as a cheap, fast, and accurate way to probe interactions between signaling and pluripotency. Additionally, undertaking in vivo work with Drosophila allows for the ability to utilize the broad range of molecular techniques in use in the organism. For example, one could utilize CRISPR/Cas9 approaches to selectively activate or inactivate homologs of the Yamanaka factors in specific tissue regions during specific stages of development.
Genes 2018, 9, 101 6 of 10 a cheap, fast, and accurate way to probe interactions between signaling and pluripotency. Additionally, undertaking in vivo work with Drosophila allows for the ability to utilize the broad range of molecular techniques in use in the organism. For example, one could utilize CRISPR/Cas9 approaches to selectively activate or inactivate homologs of the Yamanaka factors in specific tissue regions during specific stages of development. A major reason to undertake in vivo studies with Drosophila iPS cells is that, recently, Ocampo et al. showed that the systemic expression of pluripotency factors OCT4, SOX2, KLF4, and c-MYC (OSKM) could induce partial reprogramming in vivo in mice [81][82][83]. These authors observed an amelioration of cellular markers of aging and improved treatment of metabolic disease and muscle injury. These studies then provide a precedent that OSKM pluripotency factors can effectively induce a pluripotent system in primary culture and in vivo. Therefore, it should be possible to utilize this approach to create reprogrammed Drosophila organoids which could be utilized for cancer or aging studies (Figure 4), or to use adult flies to study the induction of stem cells in adults. Insect cell organoids could be made from a variety of mutant or engineered fly strains and would take advantage of the smaller genome containing fewer redundant or paralogous genes with overlapping functions. This system could then be used to test various Wnt signaling agonists and antagonists for their ability to stimulate stem cells, effectively creating an in vivo system for screens and possibly combining Wnt's role in stem cells with its role in aging [84]. A major reason to undertake in vivo studies with Drosophila iPS cells is that, recently, Ocampo et al. showed that the systemic expression of pluripotency factors OCT4, SOX2, KLF4, and c-MYC (OSKM) could induce partial reprogramming in vivo in mice [81][82][83]. These authors observed an amelioration of cellular markers of aging and improved treatment of metabolic disease and muscle injury. These studies then provide a precedent that OSKM pluripotency factors can effectively induce a pluripotent system in primary culture and in vivo. Therefore, it should be possible to utilize this approach to create reprogrammed Drosophila organoids which could be utilized for cancer or aging studies (Figure 4), or to use adult flies to study the induction of stem cells in adults. Insect cell organoids could be made from a variety of mutant or engineered fly strains and would take advantage of the smaller genome containing fewer redundant or paralogous genes with overlapping functions. This system could then be used to test various Wnt signaling agonists and antagonists for their ability to stimulate stem cells, effectively creating an in vivo system for screens and possibly combining Wnt's role in stem cells with its role in aging [84].