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Volume 3
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10.3390/genes3030361
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Communication

Genome-Wide Sequencing Reveals Two Major Sub-Lineages in the Genetically Monomorphic Pathogen Xanthomonas Campestris Pathovar Musacearum

by
Arthur Wasukira
1,2,
Johnbosco Tayebwa
1,2,
Richard Thwaites
3,
Konrad Paszkiewicz
1,
Valente Aritua
2,3,†,
Jerome Kubiriba
2,
Julian Smith
3,
Murray Grant
1 and
David J. Studholme
1,*
1
Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
2
National Crops Resources Research Institute (NaCRRI), Kampala 7084, Uganda
3
The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK
*
Author to whom correspondence should be addressed.
†
Present address: International Centre for Tropical Agriculture (CIAT), Kawanda Agricultural Research Institute, Kampala 6247, Uganda.
Genes 2012, 3(3), 361-377; https://doi.org/10.3390/genes3030361
Received: 10 June 2012 / Revised: 24 June 2012 / Accepted: 26 June 2012 / Published: 4 July 2012
(This article belongs to the Special Issue Feature Paper 2012)
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Abstract

The bacterium Xanthomonas campestris pathovar musacearum (Xcm) is the causal agent of banana Xanthomonas wilt (BXW). This disease has devastated economies based on banana and plantain crops (Musa species) in East Africa. Here we use genome-wide sequencing to discover a set of single-nucleotide polymorphisms (SNPs) among East African isolates of Xcm. These SNPs have potential as molecular markers for phylogeographic studies of the epidemiology and spread of the pathogen. Our analysis reveals two major sub-lineages of the pathogen, suggesting that the current outbreaks of BXW on Musa species in the region may have more than one introductory event, perhaps from Ethiopia. Also, based on comparisons of genome-wide sequence data from multiple isolates of Xcm and multiple strains of X. vasicola pathovar vasculorum, we identify genes specific to Xcm that could be used to specifically detect Xcm by PCR-based methods. View Full-Text
Keywords: banana Xanthomonas wilt; whole-genome sequencing; SNP; molecular markers; Ensete ventricosum; Xanthomonas vasicola banana Xanthomonas wilt; whole-genome sequencing; SNP; molecular markers; Ensete ventricosum; Xanthomonas vasicola
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MDPI and ACS Style

Wasukira, A.; Tayebwa, J.; Thwaites, R.; Paszkiewicz, K.; Aritua, V.; Kubiriba, J.; Smith, J.; Grant, M.; Studholme, D.J. Genome-Wide Sequencing Reveals Two Major Sub-Lineages in the Genetically Monomorphic Pathogen Xanthomonas Campestris Pathovar Musacearum. Genes 2012, 3, 361-377. https://doi.org/10.3390/genes3030361

AMA Style

Wasukira A, Tayebwa J, Thwaites R, Paszkiewicz K, Aritua V, Kubiriba J, Smith J, Grant M, Studholme DJ. Genome-Wide Sequencing Reveals Two Major Sub-Lineages in the Genetically Monomorphic Pathogen Xanthomonas Campestris Pathovar Musacearum. Genes. 2012; 3(3):361-377. https://doi.org/10.3390/genes3030361

Chicago/Turabian Style

Wasukira, Arthur, Johnbosco Tayebwa, Richard Thwaites, Konrad Paszkiewicz, Valente Aritua, Jerome Kubiriba, Julian Smith, Murray Grant, and David J. Studholme. 2012. "Genome-Wide Sequencing Reveals Two Major Sub-Lineages in the Genetically Monomorphic Pathogen Xanthomonas Campestris Pathovar Musacearum" Genes 3, no. 3: 361-377. https://doi.org/10.3390/genes3030361

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