Determinants of mer Promoter Activity from Pseudomonas aeruginosa

Since the MerR family is known for its special regulatory mechanism, we aimed to explore which factors determine the expression activity of the mer promoter. The Tn501/Tn21 mer promoter contains an abnormally long spacer (19 bp) between the −35 and −10 elements, which is essential for the unique DNA distortion mechanism. To further understand the role of base sequences in the mer promoter spacer, this study systematically engineered a series of mutant derivatives and used luminescent and fluorescent reporter genes to investigate the expression activity of these derivatives. The results reveal that the expression activity of the mer promoter is synergistically modulated by the spacer length (17 bp is optimal) and the region upstream of −10 (especially −13G). The spacing is regulated by MerR transcription factors through symmetrical sequences, and −13G presumably functions through interaction with the RNA polymerase sigma-70 subunit.


Introduction
Human activities constantly accelerate the emission of heavy metals into the environment [1][2][3], increasingly threatening the well-being of organisms and the ecosystem [4][5][6].According to the World Health Organization, mercury generally has the lowest maximum allowable value (MAV) among heavy metals due to its significant toxicity and bioaccumulation [7,8].To cope with the toxicity of mercury from natural sources [9][10][11], prokaryotes have evolved various mechanisms, including the well-known system of mercury resistance (mer) genes regulated by the transcription factor (TF) MerR [12][13][14][15].
Transcription is activated by inducing promoter DNA kinking and undertwisting, resulting in phasing and spacing parameters close to a 17 bp promoter [45][46][47].In addition, some studies support an optimal spacer length of 17 bp [48][49][50], while others suggest an optimal spacer length of 16-18 bp [51-53].Typically, even if the spacer differs from the optimum by only 1 bp, the promoter activity is significantly reduced [54][55][56][57][58].However, a P mer derivative with a 17 bp spacer exhibited constitutive activity similar to that of derivatives with 18 bp spacers in previous in vivo studies [27].Therefore, we became interested in what causes this phenomenon and whether the function of the base sequences in the spacer is simply for DNA distortion.
In this work, we systematically engineered mutations in four regions (−30 to −28, −25 to −23, −20 to −18, and −15 to −13) in the spacer between the −35 and −10 elements of P mer (from −31 to −13) to investigate the expression activity of the derivatives.Expression levels were analyzed semi-quantitatively by using the sensitive bioluminescent reporter genes luxAB or the visible fluorescent reporter gene sfGFP instead of the mer genes [59,60].The results indicate that the spacing (regulated through symmetrical sequence) and the region upstream of −10 (−15 to −13, especially −13G) synergistically regulate P mer activity, contributing to the further understanding of the function of the spacer sequence between the −35 and −10 elements of P mer .
Base pair deletions were designed more comprehensively in this work, ranging from 1 to 3 bp and located in the four regions (−30 to −28, −25 to −23, −20 to −18, and −15 to −13) (Figures 1B,C and S1).These positions are regularly spaced at 2 bp intervals.The −30 to −28 and −20 to −18 regions are in the symmetrical sequence ( −32 TCCGTACXXXXGTACGGA −15 ), the −25 to −23 region is between the symmetrical sequences, and the −15 to −13 region is upstream of the −10 element.The spacer length becomes 18-16 bp by deleting 1-3 bp in the spacer (Figure S2).
The merR-merOP genes and luxAB (or sfGFP) genes were amplified by PCR using Phanta ® Max Super-Fidelity DNA Polymerase.PCR amplification procedure: (1) predenaturation: 95  5) Phanta Max Super-Fidelity DNA polymerase: 1 U/50 µL.The final volume of the PCR is 50 µL.The merR-merOP genes and luxAB (or sfGFP) genes were spliced by overlapping using Phanta ® Max Super-Fidelity DNA Polymerase.The spliced fragments were integrated into plasmid pET28a by restriction endonuclease sites XbaI and EcoRI using restriction endonucleases and T4 DNA ligase to form vector pMPmerL (or pMPmerS) (Figures 1D and S3).The vectors pPmerL and pPmerS were constructed from pMPmerL and pMPmerS with the merR gene removed (Figure S3).The merOP-luxAB genes or merOP-sfGFP genes were amplified by PCR and integrated into plasmid pET28a by restriction endonuclease sites XbaI and EcoRI.The luxAB genes, merOP region, and sfGFP gene were integrated into plasmid pET28a by XbaI and EcoRI to form vector pLPmerS (Figure S3).The promoter P cpc560 and merR gene were integrated into plasmid pTf16 by SacI and XhoI to form vector pPcpc560M (Figure S3).The genes P cpcBA -sfGFP, P cpc560 -sfGFP, P psbAII -sfGFP, P rnpB -sfGFP, P tic -sfGFP, or P cmv -sfGFP were integrated into plasmid pET28a by restriction endonuclease sites XbaI and EcoRI to form pPcpcBAS, pPcpc560S, pPpsbAIIS, pPrnpBS, pPticS, and pPcmvS, respectively.Next, 2 µL of ligation product was transformed into 100 µL DH5α competent cells.The cells were grown for 16 h at 37 • C in Luria Broth (LB) agar plates (20 mg/L chloramphenicol for pTf16 or 30 mg/L kanamycin for pET28a).Single clones from LB agar plates were identified by PCR using Taq Master Mix.Positive samples were sent to Sangon Biotech for sequencing.
The derivatives P mer-M1 to P mer-M17 were generated by site-directed mutagenesis using PrimeSTAR HS DNA Polymerase.PCR amplification procedure: (1)
The vectors pMPmerL (WT and M1-M17) and pPcpc560M were cotransformed into E. coli DH5α (30 mg/L kanamycin and mg/L chloramphenicol).The subsequent steps were the same as described above except for the addition of the antibiotic chloramphenicol.
Luminescence measurements were performed in white 96-well plates with Varioskan ® Flash (Thermo Fisher Scientific Inc., Waltham, MA, USA).The wells were measured for 1 s, and the dynamic range was selected as "Auto Range".The mean and standard deviation (SD) of the three colonies tested were shown and compared by Tukey HSD (Honestly Significant Differences) test.After increasing the concentration of bacteria by centrifugation (OD600 ~1.0), luminescence images were captured for 1.1 s using an Apple iPhone 12 Pro.

Fluorescence Assays
Superfolder GFP expressed by the sfGFP gene is stable and visible, optimized for analyzing higher expression levels.The vector pMPmerS and pPmerS (WT and M1-M17) containing P mer or their derivatives were transformed into E. coli DH5α, respectively (Figure 1E).Single colonies of the WT strain and its derivatives were separately transferred from LB agar plates into liquid LB medium (30 mg/L kanamycin, mercury-free) and incubated at 37 • C until the OD600 was approximately 1.0.
Fluorescence measurements were performed using Varioskan ® Flash in 96-well UVtransparent plates with an excitation peak at 488 nm and an emission peak at 517 nm.The wells were measured for 100 ms and the bandwidth was selected to be 12 nm.After increasing the concentration of bacteria by centrifugation (OD600 ~3.0), fluorescence images were captured for 30 ms using an Apple iPhone 12 Pro.

Quantitative Reverse Transcriptase PCR (qRT-PCR) Analysis
Single colonies of the WT strain and its derivatives (P mer-M10 and P mer-M13 ) were picked from LB agar plates (30 mg/L kanamycin), inoculated separately into liquid LB medium with 30 mg/L kanamycin, and incubated at 37 • C for about 16 h.Subsequently, 100 µL of cell suspension was inoculated into 10 mL of fresh LB medium and incubated at 37 • C until the mid-exponential phase (OD600 of 0.5-0.6).The bacteria were collected by centrifugation at 5000 rpm for 5 min and total RNA was extracted using the Bacterial Total RNA Isolation Kit (Sangon).Reverse transcription of RNA was performed using the iScript™ cDNA synthesis kit.Real-time qPCR analysis was performed using SsoFast™ EvaGreen ® Supermix (Bio-Rad) on a CFX96™ Real-Time System C1000™ Thermal Cycler (Bio-Rad).The housekeeping gene 16S rRNA was used as an internal control.

Analyzing the Expression Activity
Bacteria containing different mer promoter derivatives or other constitutive promoters to express the downstream gene sfGFP were collected after overnight incubation in LB medium at 37 • C and 250 rpm.Subsequently, the gel electrophoresis of proteins was performed as described previously.The gels were imaged with ChemiDoc™ XRS+ (Bio-Rad) and the band intensity was analyzed Image Lab™ (Bio-Rad).

Construction of mer Promoter Derivatives
Derivatives M1-M17 (pPmerLM1-M17, pMPmerLM1-M17, pPmerSM1-M17, and pMPmerSM1-M17) were constructed using plasmids pPmerL, pMPmerL, pPmerS, and pMPmerS as templates (Table 1 and Figure S1).All the constructed plasmids and mutations were confirmed by DNA sequencing at Sangon Biotech.The pPmerL plasmid represents the mer promoter DopMerR expressing the luxAB genes, the pMPmerL plasmid represents the native mer promoter expressing the luxAB genes, the pPmerS plasmid represents the mer promoter DopMerR expressing the sfGFP gene, and the pMPmerS plasmid represents the native mer promoter expressing the sfGFP gene.0 0 0 15 1 The data were averaged from at least three individual experiments.Expression levels were normalized to the percentage of fully induced (50 nM HgCl 2 ) expression levels of the wild type (P mer ).

Effect of the Spacing between the −35 and −10 Elements on P mer Activity
The regulation of mer promoters (WT and derivatives M1-M17) under different MerR levels was investigated using pPmerL, pMPmerL, and pMPmerL + pPcpc560M systems (Figure 2A).Luciferase expressed by the luxAB genes reacts with decanal to generate luminescence, which is remarkably sensitive and suitable for detecting expression levels.The results showed that MerR levels had a significant effect on the 19 bp spacer mer promoter (WT and derivatives M15-M17).In the absence of MerR, the promoter was not activated; at normal MerR levels, the promoter was activated by mercury ion stimulation; at ultra-high MerR levels, the promoter was not activated by the same mercury ion stimulation.Above optimal levels, the capture of mercury ions by MerR unbound to promoter DNA may interfere with the activation of transcription by promoter-bound MerR.In luminescence assays, the level of expression of the WT construct (19 bp spacer) is very low under non-induced conditions.The addition of mercury ions significantly increased expression levels only in the presence of the MerR TF.Apparently, the MerR TF and the unusual spacer length are indispensable for this specific regulation.2).The derivatives with a 16 bp spacer merely exhibited weak constitutive expression activity, suggesting that this spacing does not allow for efficient recognition by RNA polymerase.The difference in luminescence intensity could be recognized by the naked eye in the dark (Figure S5A).The average luminescence intensity from the derivatives with a 17 bp spacer (pMPmerLM2, M5, M8, M12, and M13) was 1.86-fold higher than that from the derivatives with an 18 bp spacer (pMPmerLM1, M4, M7, M10, and M11) and 16.8-fold higher than that from the derivatives with a 16 bp spacer (pMPmerLM3, M6, M9, and M14) (Figure 3A).Among these promoters, those with a 17 bp spacer were more efficiently transcribed than those with an 18 bp spacer.S5).The average fluorescence intensity from derivatives with a 17 bp spacer (pMPmerSM2, M5, M8, M12, and M13) was 1.89-fold higher than that from derivatives with an 18 bp spacer (pMPmerSM1, M4, M7, M10, and M11) and 11.3-fold higher than that from derivatives with a 16 bp spacer (pMPmerSM3, M6, M9, and M14) (Figure S6B).Although there were slight fluctuations between the results of the luminescence and fluorescence assays, the relative intensities and trends were consistent.In addition, SDS-PAGE assays were further performed to eliminate possible errors between sfGFP expression levels and fluorescence intensity, such as those caused by protein misfolding (Figure S7).The results were in agreement with each other and demonstrated that spacer length was an important factor affecting the activity of P mer .The optimal spacer length for the expression of the target gene by P mer was 17 bp, followed by 18 bp and 16 bp.The 17-18 bp spacer mer promoter derivative had strong constitutive expression activity (Figure S8).MerR activated P mer by generating an equivalent contraction of the spacing between the −10 and −35 elements to the deletion of base pairs, once again supporting the DNA distortion mechanism regulated by MerR.
However, the model of spacer length regulating the expression level of target genes could not independently explain the similarity in expression activity of the 18 bp spacer derivative M10 (deleted −15/−14A) and the 17 bp spacer derivative M13 (deleted −14 AG −13 ) (Figure S6C).Moreover, the similarity between P mer derivative M10 and derivative M11 is not only in expression activity but also in transcriptional activity (Figure S9 and Table S6).This suggests that the abnormally long spacer region of P mer might have an additional function in the regulatory process besides the conformational distortion regulated by MerR.
For derivatives with base pair deletions in the MerR-binding symmetrical sequence region ( −31 CCGTACATGAGTACGG −16 ), there was no significant difference in expression activity between them if the spacer length was equal.At the same spacer length, the expression activity of derivatives with base pairs deleted in the symmetrical sequence was several times higher than that of derivatives with base pairs deleted in the region upstream of −10 (Figures 3B and S6D).Since no crystals of the Tn501 MerR-DNA complex have been reported and the N-terminal sequences of the MerR family TFs bound to promoter DNA are similar (Figures 4A and S10), crystals of other MerR family TFs in complex with promoter DNA (19 bp spacer) were analyzed as substitutes to characterize the structure of the spacer region.The promoter DNA was distorted around the center of the symmetrical sequence, forming a region with significant conformational differences from the B-form (Figures 4B and S11).On the other hand, the region upstream of −10 (−15 to −13) maintains a localized conformation similar to B-form DNA, although it has changed position due to the distortion [45].This means that sequence length plays a major role in regulating expression activity in the symmetrical sequence region, exactly where the promoter DNA is distorted, and in the region upstream of −10, there are additional determinants beyond sequence length involved in the regulation of expression activity.
Significantly, the expression activity of derivative M10 (deletion of −15/−14A) was 3.51-fold/3.86-fold(luminescence/fluorescence assay) higher than that of M11 (deleted −13G) at the same spacer lengths; and the expression activity of derivative M12 (deleted −15 AA −14 ) was 1.91-fold/1.89-foldhigher than that of M13 (deleted −14 AG −13 ) (Figure 3A,B).This highlighted the importance of −13G in the region upstream of −10 for the high-level expression of the target gene compared to the other positions.This finding explained the enigma of the similarity in expression activity between derivatives M13 (deleted −14 AG −13 ) and M10 (deleted −15/−14A): the 17 bp spacer in derivative M13 favored higher expression activity compared to the 18 bp spacer in derivative M10, whereas the deletion of −13G in derivative M13, in turn, reduced expression activity.The sigma factor is yellow, the promoter DNA is green, the σ2 is orange, and the RNA polymerase is grey.The base pairs at positions −12 and −13 in the promoter and the glutamine at position 437 (Q437) in σ2 are marked in purple.The yellow dotted lines show their interactions.

Role and Effects of the Regulatory Factor MerR and merR Gene
Luminescence of the derivative was detected in the pLPmerS system to study the effect of mutations in the spacer region on merR gene expression levels.The results showed that the expression level of the merR gene in the derivatives basically maintained that of the wild type (Figure S12), suggesting that the differences in expression activity of the derivatives were not caused by merR gene expression levels.
The EMSA assay was performed to determine the affinity of MerR for DNA with different mer promoters (WT and mutants M1, M4, M7, M10, M11, M12, and M14).The results showed that deletion (M1, M10, M11, M12, and M14) of the sites not within the " −29 GTACXXXXGTAC −18 " sequence had little effect on the binding of MerR to promoter DNA (Figures 3C and S13).The deletion (M4 and M7) of sites within the " −29 GTACXXXXGT AC −18 " sequence prevented MerR from binding to promoter DNA.Derivatives with similar expression activity had different affinities of their promoter DNA for MerR.It suggested that the difference in activity of the derivatives was not caused by the affinity of promoter DNA for MerR.MerR levels had no significant effect on the 16-18 bp spacer mer promoters (derivatives M1-M14), although it still bound to some promoters (Figure 2A, Figures S7  and S13) whereas changes in activity caused by the −13G site were present in all systems (Figures 3D and S6E), suggesting that −13G upstream of −10 acted as a determinant by interacting with the RNA polymerase holoenzyme and not MerR.

Conservation and Function of Guanine at Position −13 (−13G) in P mer
Our findings suggested that the abnormally long 19 bp spacer (−31 to −13) in P mer had two functions, one for MerR recognition and binding (−31 to −16) and the other for increasing downstream gene expression levels (−15 to −13) (Figure 3E).The role of guanine at position −13 (−13G) in the −15 to −13 region was critical.To explore the specificity of −13G, the derivatives pMPmerLM15 (G−13A), M16 (G−13T), and M17 (G−13C) were constructed.The fully induced expression level from the wild type (−13G) was 8.3-fold, 3.7-fold, and 6.7-fold higher than that from derivatives M15, M16, and M17 (Figure 3F).This result established that guanine at position −13 of P mer was specific to high expression levels of target genes.
The alignment of the promoter sequence regulated by the MerR family TFs with the 19 bp spacer between the −10 and −35 elements revealed that the bases at the −13 position were predominantly thymine and less often guanine (Figures 4C and S14).However, −13G was not conserved in all promoters, since these promoters are recognized by different MerR family transcription factors responding to different environmental stimuli that are not equally toxic to cells.Correspondingly, the expression level from the derivative M16 (G−13T) was 2.3-fold higher than that from M15 (G−13A) and 1.8-fold higher than that from M17 (G−13C).This indicated that the −13 base (T or G) in the promoter controlled by MerR family TFs contributed to the high expression level of the target genes.MerR family TFs regulate promoters with unusually long spacers.Notably, the symmetric sequence to which the TF binds is not centered between the −35 and −10 elements but is biased toward the −35 element.
To provide the possibility that the base at position −13 interacts with the RNA polymerase holoenzyme, we performed a crystal structure analysis.The most conserved region 2 (σ 2 ) of sigma factor 70 (σ 70 ) was divided into four subregions; subregion 2.3 (σ 2.3 ) is implicated in DNA melting, and subregion 2.4 (σ 2.4 ) is associated with recognizing the −10 element [54,75].An α-helix containing a sequence 430 YATWWIRQAITRSIAD 445 in σ 2.3 to σ 2.4 interacts with the −10 element of the promoter.Q437, which is known to bind the base at position −12, formed hydrogen bonds between the base pairs at positions −12 and −13 in partial crystals (Figure S15).Since MerR proteins lack corresponding complex crystals, we substituted CueR for MerR.Similar interactions were present in a complex crystal of the MerR homologue CueR (Figure 4D,E).Hydrogen bonding occurred between the base pairs at positions −12 (−12A') and −13 (−13T) in the promoter and the glutamine at position 437 (Q437).Thus, it was hypothesized that −13G enhances the expression activity of P mer precisely by interacting with the base pair −12T/A and the Q437 residue in σ 2.4 .In addition, both guanine and thymine contain secondary amide to form hydrogen bonds for base complementary pairing, the amide of guanine being more protruding to facilitate the formation of "mismatches" with the adenine in −12T/A (Figure S16).

Conclusions
In this paper, we analyzed the effect of the mutant spacer sequence between the −35 and −10 elements on the expression activity of the mer promoter by double-calibrated semi-quantitative analysis of luminescence and fluorescence assays.The results showed that (1) derivatives with a 3 bp deletion (16 bp spacer) had weak constitutive activity; (2) derivatives with a 1-2 bp deletion (18-17 bp spacer) were generally strong constitutive promoters; (3) 17 bp derivatives were generally stronger than 18 bp ones as well as the MerRactivated wild-type; (4) the 19 bp promoter was regulated by MerR and the constitutive expression activity of the 16-18 bp promoter was almost unaffected by MerR; (5) the derivatives retaining the sequence " −15 AAG −13 " in the upstream region of −10 showed higher expression activity at the same spacer length; (6) the activated wild-type promoter (−13G) was higher than that of the activated derivatives (G−13A, G−13T and G−13C).
The spacer deletion mutants M10 (deleted −15/−14A) and M13 (deleted −14 AG −13 ) in previous studies had similar expression activity, as the deletion of −13G partially counteracted the increase in activity caused by spacer shortening.The symmetrical sequence in the spacer region of the mer promoter, as well as other promoters regulated by MerR family TFs, was not centered between the −35 and −10 elements but was biased toward the −35 element.The base sequence upstream of the −10 element in the ultra-long spacer was not used for transcription factor binding but had additional functions.Compared to the common TG ( −15 TG −14 ) extension promoters, −13G had a different position and sequence, but both interacted with the sigma factor.Crystal structure analysis provided spatial opportunities for interactions.
Our findings support that spacer length and −13G in the region upstream of −10 work synergistically in regulating downstream gene expression levels.This study advances the understanding of the process by which the mer promoter modulates the expression levels of mer genes.

Figure 1 .
Figure 1.Schematic diagram of the expression activity assay for derivatives of the mer promoter.(A) Structure of the mer operon.(B) Sequence of the merOP region and position of mutations in the mer promoter spacer region.Hollow triangles represent reported mutants and solid triangles represent mutants in this paper.(C) Wild-type and derivative M1-M17 promoter sequences and spacer lengths.Mutation sites are labeled in pink.The −35 and −10 elements are marked with an underscore.Mutant regions are marked in pink.(D) Plasmid maps of the vectors constructed in this study.pMPmerL, pPmerL, pMPmerS, pPmerS, pLPmerS, and pPcpc560M.(E) Visible and quantitative detection of promoter expression activity in E. coli by luminescence or fluorescence assays.

Figure 3 .
Figure 3.Comparison of the properties of different promoters.(A) Comparison of luminescence intensity from derivatives with different length spacers.Data are expressed as mean ± SD, n ≥ 3 independent experiments.p-values are determined by one-way ANOVA with Tukey test, *** p < 0.001.(B) Comparison of luminescence intensity from derivatives grouped by position or number of deleted base pairs.Data are expressed as mean ± SD, n ≥ 3 independent experiments.p-values are determined by one-way ANOVA with Tukey test, * p < 0.05, ** p < 0.01, *** p < 0.001.(C) EMSA assays for WT and M11 mer promoter: 30 nM for DNA and 0, 100, 200, 300, 400, 500 and 600 nM for MerR were used in the EMSAs.(D) Comparison of luminescence intensity of P mer derivatives M10 to M14 at different MerR levels.(E) Function of the region between the −35 and −10 elements.(F) Luminescence from derivatives substituting guanine at position −13 in the mer promoter.Data are expressed as mean ± SD, n ≥ 3 independent experiments.p-values are determined by one-way ANOVA with Tukey test, * p < 0.05, ** p < 0.01, *** p < 0.001.Similar phenomena were observed from derivatives M1 to M14 (pPmerS, pMPmerS, and pMPmerS + pPcpc560M systems) in the fluorescence assays (Figures S5B, S6A, S7, and TableS5).The average fluorescence intensity from derivatives with a 17 bp spacer (pMPmerSM2, M5, M8, M12, and M13) was 1.89-fold higher than that from derivatives with an 18 bp spacer (pMPmerSM1, M4, M7, M10, and M11) and 11.3-fold higher than that from derivatives with a 16 bp spacer (pMPmerSM3, M6, M9, and M14) (FigureS6B).Although there were slight fluctuations between the results of the luminescence and fluorescence assays, the relative intensities and trends were consistent.In addition, SDS-PAGE assays were further performed to eliminate possible errors between sfGFP expression levels and fluorescence intensity, such as those caused by protein misfolding (FigureS7).The results were in agreement with each other and demonstrated that spacer length was an important factor affecting the activity of P mer .The optimal spacer length for the expression of the target

Figure 4 .
Figure 4. Alignment of the promoter −13 base.(A) Similarity in the N-terminal structure of MerR family transcription factors.BmrR (PDB: 7CKQ) is blue, CadR (PDB: 6JGX) is yellow, CueR (PDB: 1Q05) is purple, EcmrR (PDB: 6XL6) is green, MerR (PDB: 5CRL) is red, MtaN (PDB: 1R8D) is cyan, and PbrR (PDB: 5GPE) is orange.(B) Structure of promoter DNA distortion regulated by MerR family transcription factors.(C) Sequence alignment between the −10 and −35 elements of promoters regulated by MerR family TFs.(D) Complex crystals of activated forms of CueR (a homologue of MerR), promoter DNA, and RNA polymerase holoenzyme (PDB: 6XH7).CueR is indicated by the line, the sigma factor is yellow, the promoter DNA is green, and the σ2 is orange.The RNA polymerase is grey.(E) Interactions of base pair at position −13 in the promoter regulated by CueR.The sigma factor is yellow, the promoter DNA is green, the σ2 is orange, and the RNA polymerase is grey.The base pairs at positions −12 and −13 in the promoter and the glutamine at position 437 (Q437) in σ2 are marked in purple.The yellow dotted lines show their interactions.
Sequence and spacer length of mer promoter derivatives.The −35 element is purple and the −10 element is pink.Mutation sites are marked with dotted boxes on a yellow background.Figure S2 Scheme of the DNA conformation of the region between the −35 and −10 elements of mer promoter derivatives.The −35 element is purple, the −10 element is pink, and the intervening spacer is green.Figure S3 Plasmid maps of the vectors constructed in this study.(A) pPmerL, (B) pMPmerL, (C) pPmerS, (D) pMPmerS, (E) pLPmerS, (F) pPcpc560M.Figure S4 Mercury ion concentration affects bacterial growth and luminescence intensity.(A) Toxicity of mercury ions (0-100 µM) to Escherichia coli.(B) Toxicity of mercury ions (0-100 nM) to E. coli containing the pMPmerL plasmid at different times.(C) Mercury ions (0-100 nM) induced luminescence in E. coli containing the pMPmerL plasmid (linear coordinates).(D) Mercury ions (0-100 nM) induced luminescence in E. coli containing the pMPmerL plasmid (logarithmic coordinates).Figure S5 Visualization of derivative expression activity.(A) Visualization of the difference in luminescence levels between the derivatives M1-M14 of the pMPmerL plasmid.(B) Visualization of the difference in fluorescence levels between the derivatives M1-M14 of the pMPmerS plasmid.Figure S6 Expression activity of mer promoter derivatives.(A) Fluorescent gene expression levels from derivatives M1 to M14 of the pMPmerL plasmid.(B) Comparison of fluorescence intensity from derivatives with different length spacers.(C) Similarity of promoter activity between derivative M10 and derivative M13.(D) Comparison of fluorescence intensity of derivatives grouped by position or number of deleted base pairs.(E) Comparison of fluorescence intensity of P mer derivatives M10 to M14 in the presence and absence of MerR.
Figure S13 MerR binding to promoter DNA of wild type and derivatives.Non-activated B-form is red, activated distortion-form is green.The promoter DNA fragments contain −35 to −10 elements and their spacer length ranges from 16-19 bp.30 nM for DNA and 0, 100, 200, 300, 400, 500 and 600 nM for MerR were used in the final EMSA experiment.Figure S14 Sequence alignment of promoters with 19 bp spacer between −10 and −35 elements regulated by MerR family TFs.The −35 and −10 elements are marked with a double underline.Symmetrical sequences are shown in bold.

Table 1 .
Relative expression levels from P mer and P mer derivatives.