MALAT1: A Long Non-Coding RNA with Multiple Functions and Its Role in Processes Associated with Fat Deposition

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) belongs to the lncRNA molecules, which are involved in transcriptional and epigenetic regulation and the control of gene expression, including the mechanism of chromatin remodeling. MALAT1 was first discovered during carcinogenesis in lung adenocarcinoma, hence its name. In humans, 66 of its isoforms have been identified, and in pigs, only 2 are predicted, for which information is available in Ensembl databases (Ensembl Release 111). MALAT1 is expressed in numerous tissues, including adipose, adrenal gland, heart, kidney, liver, ovary, pancreas, sigmoid colon, small intestine, spleen, and testis. MALAT1, as an lncRNA, shows a wide range of functions. It is involved in the regulation of the cell cycle, where it has pro-proliferative effects and high cellular levels during the G1/S and mitotic (M) phases. Moreover, it is involved in invasion, metastasis, and angiogenesis, and it has a crucial function in alternative splicing during carcinogenesis. In addition, MALAT1 plays a significant role in the processes of fat deposition and adipogenesis. The human adipose tissue stem cells, during differentiation into adipocytes, secrete MALAT1 as one the most abundant lncRNAs in the exosomes. MALAT1 expression in fat tissue is positively correlated with adipogenic FABP4 and LPL. This lncRNA is involved in the regulation of PPARγ at the transcription stage, fatty acid metabolism, and insulin signaling. The wide range of MALAT1 functions makes it an interesting target in studies searching for drugs to prevent obesity development in humans. In turn, in farm animals, it can be a source of selection markers to control the fat tissue content.


Long Non-Coding RNAs
Long non-coding RNAs are molecules longer than 200 nucleotides and are divided into intronic and intergenic ncRNAs, sense lncRNAs, anti-sense lncRNAs, enhancer-associated lncRNAs, and circular lncRNAs [1].Previously, they were considered not to encode proteins, but it has recently been reported that most contain open reading frames and hat they are translated [2].Early literature positions held that lncRNAs could be converted into small proteins or micro-peptides, but these peptides are often highly unstable structures and mostly lack biological functions [3].However, recently, lncRNA-derived peptides have become hot topics owing to their functionality in carcinogenesis, cancer progression, and the immune response [4,5].Meanwhile, regarding tissue specificity, numerous studies have suggested that lncRNAs are the most abundant in the testis [6] and neural tissue.
In 2017, the FANTOM5 project identified 28,000 lncRNAs when using different human sources [7].Initial studies suggested that lncRNAs are highly conservative in their sequences in different species.They are poor in rare variants [8] and mutations of insertion/deletion types [9].However, it was recently reported that lncRNAs are strongly conserved only at the genome stage and not at the transcript level, which means that they Genes 2024, 15, 479 2 of 16 are not transcribed in the orthologous genomic region, which may be associated with rapid species-specific adaptive selection [10].Studies of lncRNA function are still insufficient, although evidence shows that most lncRNAs in mammals are likely to be functional [6].Nevertheless, their biological relevance has been presented for only a few species.
In humans, approximately 2600 lncRNAs have been annotated as functional, a number that is much lower in other vertebrate species [11].In the literature, it is described that lncRNAs are involved in transcriptional and epigenetic regulation.Overall, they control gene expression and the cell cycle, including the mechanisms of chromatin remodeling [12] and miRNA sponging to relieve or inhibit the binding action of miRNA with target transcripts [13].Finally, some lncRNAs expressed from enhancer or silencer regions can bind to target transcripts and enhance [14] or inhibit [15] their subsequent translation.Therefore, it can be said that these molecules are essential regulators of gene expression because they can change gene expression conditions at various molecular levels.Moreover, large-scale investigations considering numerous molecules provide evidence that lncRNAs are, in fact, peptide-coding [16].Consequently, providing information about newly described lncRNAs and confirming their biological function using in vitro methods seem necessary, especially in animals, which are studied less frequently than humans.
In a recent study, Piórkowska et al. [23] identified an lncRNA (ENSSSCG00000048856) within the subcutaneous fat transcriptome of pigs, which was differentially expressed between individuals with low and high subcutaneous fat deposition highly conserved with the human MALAT1 gene.Porcine MALAT1 was previously described by Yang et al. in 2017 [24], who found that the identified CUFF.253988.1 lncRNA shared homology with the human MALAT1.However, in Ensembl databases, this lncRNA still appears without names but as a novel gene.This gene in pigs is located on chromosome 2: at 6,751,519-6,757,180 encoded at the reverse strand (according to Sscrofa11.1,Ensembl Release 111, January 2024), and it is predicted that it has two isoforms, the longest being 3503 bp, but studies on MALAT1 in pigs are few in number, so these observations need to be extended.The porcine genomic sequence of MALAT1 reveals homology with the human MALAT1 at 83% and the mouse MALAT1 at 78% (Blast NCBI).Moreover, Piórkowska et al. [23], when testing the subcutaneous fat tissue transcriptome, concluded that porcine MALAT1 has additional isoforms, which, for example, correspond to human MALAT1-201, the longest isoform.Therefore, the state of porcine MALAT1 isoforms still seems to be unsolved.
Although MALAT1 has a genomically encoded poly(A) tract, during post-transcriptional processing, the poly(A) tail is missing [25].The authors described RNase P as cleaving the primary MALAT1 transcript downstream of the genomically encoded polyA-rich tract to, in parallel, generate 3 ′ of mature MALAT1 transcript and 5 ′ of small tRNA-like molecules.The resultant 3 ′ end of the nuclear MALAT1 transcript post-processing is not polyadenylated, but it contains a genomically encoded poly(A)-rich stretch.The long MALAT1 transcript is localized to nuclear speckles [26] and the small t-RNA-like MALAT1 in the cytoplasm [25].The small MALAT1 molecule has a triple-helix structure [27], which is highly stable in cancer cells and less stable in other cell cultures [28].This triple-helical structure confers stability and nuclear localization in the absence of a true polyA tail.Moreover, MALAT1 is known to be misregulated in many human cancers [29].
MALAT1 is expressed in numerous tissues according to the ENCODE project [30], with high abundance in the adipose tissue, adrenal gland, heart, kidney, liver, ovary, pancreas, sigmoid colon, small intestine, and spleen; less in the testis and lung; and low expression in the brain.The Roslin Institute investigated the transcriptome of male and female pigs and identified enriched MALAT1 expression in numerous tissues (https:// www.ensembl.org/Sus_scrofa/Gene/ExpressionAtlas?db=core, accessed on 15 November 2023; g = ENSSSCG00000048856; r = 2:6751519-6757180).Moreover, Piórkowska et al. [23] showed that porcine MALAT1 expression in subcutaneous fat tissue was positively correlated with the thickness of backfat, making MALAT1 highly interesting in the context of adipogenesis and fat-deposition-related processes.

Upstream Regulation of MALAT1 Gene Expression
MALAT1 lncRNA was thoroughly investigated in the context of cancer prognosis/entities and metastasis, so examples of the upstream regulation of MALAT1 transcription are tightly associated with cancer-related processes.In hepatocellular carcinoma (HCC), Huang et al. [31] observed that the expression of transcription factors Sp1 and Sp3 correlated with MALAT1 expression, and the co-silencing of both TFs repressed transcription of this lncRNA, which highlights the positive regulation of MALAT1 expression by these transcription factors.In a study analyzing PCDH10 function in the context of tumor suppression, Zhao et al. [32] observed that overexpression of PCDH10 in AN3CA and HEC-1-B cell lines significantly downregulated MALAT1 expression, which was correlated with cell proliferation.The authors proved that this suppression is mediated by the canonical Wnt/β-catenin signaling pathway.Moreover, a study of mice reported that MALAT1 expression was induced by hypoxia [33], and further analysis identified that this condition and its regulation are involved in the CaMKK/AMPK/HIF-1α axis [34], which is strongly associated with Ca 2+ inputs for the augmentation of the MALAT1 promoter during hypoxia.Moreover, it was observed that during the malignant transformation of human hepatic epithelial cells induced by arsenite, MALAT1 and hypoxia-inducible factor (HIF)-2α created a feedback loop, because MALAT1 causes dissociation of von Hippel-Lindau (VHL) protein from HIF-2α, which leads to the accumulation of this protein, and then HIF-2α regulates the transcription of MALAT1 [35].In turn, during oxidative stress conditions in endothelial cells under H2O2 exposure, it was found that MALAT1 transcription could be induced by the p53 protein [36], the regulation of which in mice was previously suggested [37].
In other cancer research aiming to develop a therapeutic target for the treatment of Ewing sarcoma (EWS), it was found that MALAT1 transcription was dependent on spleen tyrosine kinase (SYK)-mediated signaling, and c-MYC TF promoting SYK's binding to the MALAT1 promoter, which enhanced the proliferation of EWS [38].In addition, in colorectal and gastric (GC) cancer cultures, it was observed that silencing of Yes-associated protein 1 (YAP1), which plays a significant role in the development of numerous carcinomas, led to the downregulation of MALAT1 expression [39].The role of MALAT1 in therapy for multiple myeloma (MM) was investigated by Amodio et al. [40], who determined that MALAT1 is entangled in a positive feedback loop with NRF1 and NRF2 TFs modulated by KEAP1, which suggests that targeting MALAT1 will offer a novel powerful option for the treatment of MM.NRF1 is a key regulator of the proteasome bounce-back response, and its inhibition sensitizes cancer cells to proteasome inhibitors [41].In turn, proteasome inhibitors are believed to be promising drugs for the treatment of proteasome-activated cancers such as MM [42].Meanwhile, NRF2 has been shown to be associated with the malignant phenotype among all myeloma cells [43].In a study investigating the influence of the SOX17 protein in esophageal squamous cell carcinoma (ESCC), it was observed that human MALAT1 contains an SRY element in its promoter, which is associated with SOX17 via TF binding [44], and then the authors suggested that SOX17 significantly limits MALAT1 expression.Moreover, in the same cancer entity, it was observed that, post-transcriptionally, MALAT1 molecules can be regulated by miR-101 and miR-217 [45], leading to MALAT1 silencing and suppressing the proliferation of ESCC cells by arresting the G2/M cell cycle.In turn, Koshimizu et al. [46] reported that during neuroblastoma development, MALAT1 expression is sensitive to the activation of oxytocin cell surface receptors, and this induction of gene expression probably occurs through the cyclic AMP-responsive element binding (CREB) transcription factor, the binding site for which was identified in the MALAT1 promoter.Furthermore, during bladder cancer, MALAT1 was upregulated by TGF-β, which promotes tumor invasion and metastasis [47], and targeted inhibition of MALAT1 suppressed the migration and invasion properties of TGF-β.
MALAT1 expression and upregulation during liver regeneration were also investigated [48], and it was concluded that MALAT1 plays a significant role in accelerating cell cycle progression in hepatocytes and promoting proliferation in vitro.It was observed that the hepatocyte growth factor increased MALAT1 expression and that the p53 TF was involved in the negative regulation of MALAT1 during liver regeneration.In other studies, MALAT1 expression was stimulated in the kidneys of diabetic mice by a high increase in glucose, which is positively related to serum creatinine and urinary albumin levels [49,50].Returning to post-transcriptional regulation, Leucci et al. [51] used the L428 and U87MG cell lines to prove that miR-9 regulates MALAT1 expression mediated by AGO2.
The downstream regulations of MALAT1 are described further in this paper, considering its significant role in numerous crucial processes.

MALAT1 in Cell Cycle Regulation
The correct course of the cell cycle leading to the duplication of genetic information is the basis for maintaining cellular homeostasis, and its regulation, mainly through checkpoint pathways, includes cell quiescence, proliferation, and apoptosis [52].Many studies have focused on examining the impacts of the expression levels of specific genes, including MALAT1, on the regulation of the cell cycle, mainly in the process of carcinogenesis, which involves uncontrolled proliferation and inhibition of the apoptosis of cancer cells.Numerous studies have shown that MALAT1 has pro-proliferative effects, and high cellular levels of MALAT1 have been observed during the G1/S and mitotic (M) phases [53,54].Silencing MALAT1 activity via two microRNAs, miR-101 and miR-217, as previously mentioned, leads to cell cycle arrest in the G2/M phase, probably through changes in the expression of p21, p27, and B-MYB [45].Previously, BrdU-PI flow cytometry analysis showed that MALAT1 depletion resulted in reduced replication and increased expression of the p53, p21, p27, and cyclin-2 genes, which are key to cell cycle inhibition.Subsequently, gene expression analysis of human diploid fibroblasts (HDFs) with MALAT1 knockout showed reduced expression of genes involved in the transition from the G1 to the S phase (CCNA2, CDC25C, Cdk1, E2F2, and MCM6), replicative progression (Cdc45, Cdt1, GINS2, GMNN, MCM3, and MCM10), and mitotic progression (such as AURKA, AURKB, BIRC5, and BUB1) [53].Similarly, knockout of the MALAT1 gene resulted in a delay in the transition from the G1 to the S phase in LNCaP cells and reduced expression of the cyclin D1 (CCDN1) and CDK6 proteins, which are important at the G1/S restriction point [55].Furthermore, the depletion of MALAT1 resulted in decreased levels of B-MYB, which localizes to the promoters of genes that are expressed during the M phase, resulting in the aberrant expression of these genes [56].Moreover, the interaction of MALAT1 with the nuclear protein hnRNP C has been shown to support the translocation of MALAT1 from the nucleus to the cytoplasm, promoting the transition from the G2 to the M phase [57].On the other hand, many studies have indicated the role of MALAT1 in the resistance of cancer cells to chemotherapy in chronic myeloid leukemia, head and neck squamous cell carcinoma, and hepatocellular carcinoma [58], increasing the ability to repair DNA, evade cell cycle checkpoints, and regulate apoptosis, autophagy, and stemness of cancer cells.Moreover, MALAT1 stimulates the cell cycle of liver cells.In this tissue, knockout of MALAT1 causes prolongation of the G0/G1 phase, and its overexpression resulted in an increased number of cells in the replication phase and a decreased number in the G0/G1 phase [48].
However, Du et al. [59] reported that MALAT1 overexpression inhibits the cell cycle in the G0/G1 phase and promotes apoptosis in endothelial cells.Moreover, flow cytometry of breast cancer (BC) cells with MALAT1 knockout showed an increased number of cells in the G0/G1 phase, with a simultaneous decrease in the number of cells in the S phase [60].The knockout of the MALAT1 gene in esophageal cancer cells (ESCCs) resulted in an increased number of cells in the G2/M phase and activation of the ATM-CHK2 pathway, the role of which is to prevent too-rapid tumor growth by inhibiting the G2/M phase [61] (Figure 1).
in the aberrant expression of these genes [56].Moreover, the interaction of MALAT1 with the nuclear protein hnRNP C has been shown to support the translocation of MALAT1 from the nucleus to the cytoplasm, promoting the transition from the G2 to the M phase [57].On the other hand, many studies have indicated the role of MALAT1 in the resistance of cancer cells to chemotherapy in chronic myeloid leukemia, head and neck squamous cell carcinoma, and hepatocellular carcinoma [58], increasing the ability to repair DNA, evade cell cycle checkpoints, and regulate apoptosis, autophagy, and stemness of cancer cells.Moreover, MALAT1 stimulates the cell cycle of liver cells.In this tissue, knockout of MALAT1 causes prolongation of the G0/G1 phase, and its overexpression resulted in an increased number of cells in the replication phase and a decreased number in the G0/G1 phase [48].
However, Du et al. [59] reported that MALAT1 overexpression inhibits the cell cycle in the G0/G1 phase and promotes apoptosis in endothelial cells.Moreover, flow cytometry of breast cancer (BC) cells with MALAT1 knockout showed an increased number of cells in the G0/G1 phase, with a simultaneous decrease in the number of cells in the S phase [60].The knockout of the MALAT1 gene in esophageal cancer cells (ESCCs) resulted in an increased number of cells in the G2/M phase and activation of the ATM-CHK2 pathway, the role of which is to prevent too-rapid tumor growth by inhibiting the G2/M phase [61] (Figure 1).
Changes in the course of the cell cycle due to different expression levels of MALAT1 depend on the type of cell.Cancer cells are characterized by an uncontrolled cell cycle as a result of mutations in tumor suppressor genes' or oncogenes' activity, as opposed to normal cells.Moreover, the type of cancer or the unique combination of genetic changes may provide different explanations of the impact of MALAT1 expression on cell cycle changes.Changes in the course of the cell cycle due to different expression levels of MALAT1 depend on the type of cell.Cancer cells are characterized by an uncontrolled cell cycle as a result of mutations in tumor suppressor genes' or oncogenes' activity, as opposed to normal cells.Moreover, the type of cancer or the unique combination of genetic changes may provide different explanations of the impact of MALAT1 expression on cell cycle changes.

Diseases Related to the MALAT1 Gene
Though most of the disease entities related to MALAT1 are associated with different kinds of cancers, it is also discussed in other disorders, such as liver fibrosis or diabetes.
Many studies have focused on cancers that occur in women, such as vulvar squamous cell carcinoma (VSCC), endometrioid endometrial carcinoma (EEC), epithelial ovarian cancer (EOC), and breast cancer.Increased expression of MALAT1 has been confirmed in lobular breast cancer [62].In EOC, increased MALAT1 expression levels have been associated with distant metastases, and many studies indicate that MALAT1 is involved in the process of metastasis [63].It was shown that HPV-positive cells expressed MALAT1, but tissues affected by VSCC, a disease correlated with HPV, showed reduced levels of MALAT1 expression [62,63].In the course of EEC, the PCDH10-Wnt/β-catenin-MALAT1 regulatory axis is a crucial component, and protocadherin 10 (PCDH10), a tumor suppressor gene, decreases the level of MALAT1, which is correlated with the inhibition of tumorigenesis [32].MALAT1 interacting with miR-218 was found to promote the proliferation of human choriocarcinoma cells by regulating the function of oncogenic F-box/WD repeat-containing protein 8 (Fbxw8) in phosphorylation-dependent ubiquitination [64].
Furthermore, numerous studies have focused on cancers of the digestive system, such as gastric cancer (GC) and colorectal cancer (CRC).The stimulatory effect of MALAT1 on cancer cell proliferation in GC has been demonstrated [65]; moreover, Qi et al. [66] reported that MALAT1 inhibits the anti-oncogene PCDH10, stimulating the proliferation of stomach cancer cells and metastasis.In the course of CRC, MALAT1 stimulates the proliferation, invasion, and migration of cancer cells through PRKA kinase anchor protein 9 (AKAP-9), which is associated with cancer progression and metastasis [67].The knockout of MALAT1 was found to inhibit the proliferation of human tongue cancer cells and the metastasis process and increase the level of miR-124, which regulates the expression of jagged1 (JAG1), which, in turn, stimulates the proliferation of cancer cells [68].
Increased expression of MALAT1 in the course of hilar cholangiocarcinoma (HCCA) was correlated with the pathological T stage of the tumor (according to the TNM classification), nerve invasion, and increased tumor area.Moreover, it was established that MALAT1 is involved in oncogenesis by regulating CXCR4, which depends on mir-204 [69].Upregulation of MALAT1 has also been observed in other types of cancer, such as bladder epithelial cancer, with high malignant potential [70].Increased MALAT1 expression was demonstrated in infantile hemangioma (IH).Moreover, knockdown of MALAT1 induced the process of apoptosis and cell cycle arrest in the replication phase in human umbilical vein endothelial cells (HUVECs) [71].Increased MALAT1 expression was demonstrated in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML), and knockout of MALAT1 in AML was found to inhibit proliferation by arresting the cell cycle in the G0/G1 phase and promote apoptosis of cancer cells by increasing the expression of caspase-3, -8, and -9 proteins [58,72].
However, changes in MALAT1 expression have also been observed in other types of diseases, such as hyperglycemia, diabetic retinopathy, proliferative vitreoretinopathy, liver fibrosis, and hypertension.MALAT1 regulates the expression of the cytokines interleukin 6 (IL-6) and tumor necrosis factor-alfa (TNF-α) by activating serum amplitude antigen 3 (SAA3) in arterial hyperglycemia [73].Furthermore, increased expression of MALAT1 has been demonstrated in RF/6A hyperglycemic model cells and the aqueous humor and fibrovascular membranes of patients suffering from diabetic retinopathy, a common complication of diabetes [74].Increased MALAT1 expression has also been observed in blood and fibrovascular membranes in another eye disease, proliferative vitreoretinopathy [75].MALAT1 overexpression promotes lipid accumulation, hepatic steatosis, and insulin resistance by increasing the expression of SREBP-1c, a sterol regulatory binding protein that is activated mainly by insulin, and the target genes ACC1, ACLY, SCD1, and FAS [76].Increased MALAT1 expression in liver fibrosis and regulation of botulinum C3 substrate 1 (Rac1) expression by miR-101b have been demonstrated to affect the cell cycle and activation of primary hepatic stellate cells.
On the other hand, it was found that the single nucleotide polymorphism rs619586A>G in MALAT1 is associated with the risk of pulmonary arterial hypertension, increasing the expression of X-box binding protein 1 (XBP1), which inhibits the proliferation of vascular endothelial cells [77].

MALAT1 Is Involved in Molecular Process Related to Carcinogenesis and Cancer Progression
MALAT1's role in cancer biology is performed via the regulation of important signaling pathways that are involved in rudimentary processes like cell division or maintaining cell identity.As the full name of MALAT1 suggests, this lncRNA influences cancer metastasis.One of the related pathway modes of action of MALAT1 in metastasis is the Wnt/β-catenin pathway, which is activated by MALAT1 overexpression.In detail, the 3 ′ end of MALAT1 directly interacts with the N-terminal of EZH2 [78], thereby increasing its expression [78,79].The upregulated EZH2 promotes GSK-3β downregulation by lysine 27 tri-methylation of histone 3. Through GSK-3β downregulation, β-catenin ubiquitination is inhibited, and the Wnt/β-catenin pathway is thereby activated [79].However, in bladder cancer, researchers indicate that MALAT1 interacts with suz12, another PRC2 component, instead of EZH2 [47].In NK and T cell lymphomas, in turn, MALAT1 was shown to interact with both PRC2 components [80].Bioinformatic analysis of publicly available breast cancer datasets of protein-DNA and ncRNA-DNA binding interactions has identified 1293 genomics regions shared by PRC2 and MALAT1.The importance of the interaction was highlighted by GO enrichment analysis results, as the identified regions proved to be related to cancer malignancy [81].Liang et al. [82] have shown that blocking Wnt/βcatenin, while simultaneously overexpressing MALAT1, abolishes the effects seen in a transwell migration assay and changes the expression profile of EMT markers typical for the mesenchymal phenotype.Apart from EMT markers' regulation, the activation of the Wnt/β-catenin path by MALAT1 leads to the upregulation of genes like c-Myc, cyclinD1, MMP-7, and CD44 [83], which are also linked to metastasis [83][84][85][86].Another pathway related to MALAT1 in the metastasis context is PI3K-AKT.Jin et al. [87] have found that by activating the PI3K-AKT pathway, MALAT1 regulates EMT markers' expression, namely E-cadherin, N-cadherin, vimentin, snail42, MMP2, and MMP9.The mechanism by which MALAT1 influences the PI3K-AKT pathway has been described by Peng et al. [88], who demonstrated that MALAT1 lowers the expression of miR-146a by sponging, which, in turn, downregulates the pathway by targeting the 3 ′ UTR of PI3K mRNA.
Wang et al. [95] imply that the cisplatin chemoresistance of oral squamous cell carcinoma cells is promoted by MALAT1-induced activation of PI3K-AKT-mTOR, which leads to growth factors' upregulation, like VEGF, c-myc, and survivin [21].In agreement with the above, the levels of VEGF, as well as another pro-angiogenic factor, FGF2, were found to be increased by the MALAT-1/mTOR/HIF-1α pathway, thereby promoting angiogenesis and ipso facto tumor growth [96].
Alternative splicing events that take place in cancer cells differ from those in healthy cells.Apart from splicing factors (SFs) like, for example, the serine-/arginine-rich family of nuclear phosphoproteins (SRs) or heterogeneous nuclear ribonucleoparticle proteins (HnRNPs), alternative splicing (AS) is also influenced by lncRNAs, for instance, DGCR5 or LINC01232.An lncRNA can interact with an SF to protect them from degradation, direct their transport, or maintain interactions between different types of SFs.The MALAT1 abundance in nuclear speckles, that is, the locations within the nucleus where splicing factors are assembled, suggests that this lncRNA is somehow significant in splicing events [97].Indeed, MALAT1, like other lncRNAs, has been shown to be involved in AS by interacting with SFs to favor splicing variants that promote cancer development due to anti-apoptotic (BIM, BIN1) and pro-proliferative properties (TEAD1) [98].The role of MALAT1 was shown to be necessary for two splicing factors to bind-PTBP1 with PSF.The interaction between the three above-mentioned elements was found to be interdependent for the generation of a malignant phenotype (cell growth, invasion, migration) in hepatocellular carcinoma cells [99].
MALAT1 overexpression is linked with a poor cancer prognosis, including in breast cancer [100], non-small-cell lung cancer [101], and glioma [102].Accordingly, the MALAT1 polymorphisms have been examined in regard to cancer risk contribution.Among the SNPs, there are cancer-risk-susceptibility as well as protective variants, as was demonstrated, for instance, in gastric cancer [103], papillary thyroid cancer [104], and hepatocellular carcinoma [105], as well as for cancer generally [106].The correlation between specific polymorphisms and cancer risk might be explained by the alternations in MALAT1 expression.In the rs664589 variant, the G allele impacts miR-194-5p binding and, in the aftermath, MALAT1 degradation by Ago2 is inhibited, leading to its upregulation [107].
Overexpression of MALAT1 often contributes to a wide array of changes within the cell.The mode of MALAT1 action in cancer is multifold since it interacts with an array of microRNAs, as well as with mRNA and proteins, leading to significant changes within signaling pathways that, in turn, more or less contribute to the cancer phenotype.That being so, MALAT1 should be highlighted in further research, as it emerges as a promising target for cancer therapies, including gene therapies.

Role of MALAT1 in Processes Associated with Fat Deposition and Adipogenesis
Adipose tissue is a special type of connective tissue that is dominated by fat cells (adipocytes).Adipocytes can occur either singly or in groups in the connective tissue, but most often the cells come together in large clusters to form adipose tissue distributed throughout the body.An excessive accumulation of adipose tissue can lead to various metabolic diseases [108].
Obesity is a very strong factor that can predispose an individual to cardiovascular disease [109], diabetes [110], hypertension [111], and cancer [112].The obesity epidemic is occurring not only in developed countries but also in developing countries.Obesity results from an imbalance between energy intake and consumption.Recently, research and scientific interest has been increasingly focused on the possible role of lncRNAs in obesity [113].
These studies attempt to explain the role of lncRNAs in obesity-related and fat deposition (FD) processes in humans and animals.Sun et al. [114] identified 1932 lncRNAs in adipose tissue (AT) and suggested that lnc_000414 is related to fat synthesis by inhibiting the proliferation of intracellular adipocytes.Another research team compared the backfat of Duroc and Chinese Luchuan pigs and found lncRNAs associated with 13 AT-related quantitative trait loci [115].A recent study indicated that LncIMF4 controls adipogenesis in intramuscular preadipocytes by relieving autophagy to inhibit lipolysis [116].In turn, our previous study showed that one lncRNA, MALAT1, selected based on differentially expressed gene analysis, may be a potential regulator of processes related to fat deposition, which we surmised because it showed an increased expression in porcine AT, which was dependent on backfat accumulation [23].In this study, we identified eight DE lncRNAs.However, orthologs for only two (MALAT1 and GLIS1) were found in other species (BLAST analysis), while the rest were pig-specific.
The data in the literature are diverse and controversial regarding the role of MALAT1 in obesity and related disorders [117] (Figure 2).MALAT1 is one of the most abundant lncRNAs identified in the exosomes of human adipose-tissue-derived stem cells (hADSCs).When hADSCs begin to differentiate into adipocytes, most MALAT1 lncRNA is retained by preadipocytes and adipocytes [118].Researchers have observed a significant reduction in MALAT1 in visceral white adipose tissue (vWAT) in aged mice [117].However, the significance of this lncRNA in adipose tissue remains uncertain.
in obesity and related disorders [117] (Figure 2).MALAT1 is one of the most abundant lncRNAs identified in the exosomes of human adipose-tissue-derived stem cells (hADSCs).When hADSCs begin to differentiate into adipocytes, most MALAT1 lncRNA is retained by preadipocytes and adipocytes [118].Researchers have observed a significant reduction in MALAT1 in visceral white adipose tissue (vWAT) in aged mice [117].However, the significance of this lncRNA in adipose tissue remains uncertain.MALAT1 is a highly expressed lncRNA that affects the regulation of various physiological and pathological processes in many tissues.Various studies, and especially [119], have found that MALAT1 is mainly localized in the nucleus, and in patients with cancerrelated cachexia, MALAT1 is downregulated in white adipose tissue (WAT) nuclei, which is associated with a low fat mass and a poor prognosis for cancer [119].Other experiments have shown that MALAT1 expression in fat tissue is positively correlated with the expression of the FABP4 and LPL regulatory genes.These data indicate that MALAT1 causes and enhances fat tissue formation.In addition, MALAT1 regulates PPARγ gene expression and participates in adipogenesis at the transcriptional level through the PPAR signaling pathway, and it participates in fatty acid metabolism and insulin signaling [119].
Previous studies showed that MALAT1 expression was lower in subcutaneous adipose tissue in obese mice [117], while higher levels of MALAT1 were observed in adiposetissue-derived stem cells from obese animals [120].In contrast, more recent studies indicate that the level of MALAT1 is reduced in the white adipose tissue of obese mice, while its deletion has neither a stimulatory nor inhibitory effect on diet-induced fat gain and lipid homeostasis in obese mice [121].A positive correlation between MALAT1 gene expression and insulin resistance was observed in the subcutaneous adipose tissue (SAT) of patients, suggesting the involvement of lncRNAs in the pathogenesis of obesity [121].However, the transcription levels of MALAT1 and TUG1 showed a positive correlation with major lipogenic and adipogenic genes [121], and thus the authors suggested possible MALAT1 is a highly expressed lncRNA that affects the regulation of various physiological and pathological processes in many tissues.Various studies, and especially [119], have found that MALAT1 is mainly localized in the nucleus, and in patients with cancer-related cachexia, MALAT1 is downregulated in white adipose tissue (WAT) nuclei, which is associated with a low fat mass and a poor prognosis for cancer [119].Other experiments have shown that MALAT1 expression in fat tissue is positively correlated with the expression of the FABP4 and LPL regulatory genes.These data indicate that MALAT1 causes and enhances fat tissue formation.In addition, MALAT1 regulates PPARγ gene expression and participates in adipogenesis at the transcriptional level through the PPAR signaling pathway, and it participates in fatty acid metabolism and insulin signaling [119].
Previous studies showed that MALAT1 expression was lower in subcutaneous adipose tissue in obese mice [117], while higher levels of MALAT1 were observed in adipose-tissuederived stem cells from obese animals [120].In contrast, more recent studies indicate that the level of MALAT1 is reduced in the white adipose tissue of obese mice, while its deletion has neither a stimulatory nor inhibitory effect on diet-induced fat gain and lipid homeostasis in obese mice [121].A positive correlation between MALAT1 gene expression and insulin resistance was observed in the subcutaneous adipose tissue (SAT) of patients, suggesting the involvement of lncRNAs in the pathogenesis of obesity [121].However, the transcription levels of MALAT1 and TUG1 showed a positive correlation with major lipogenic and adipogenic genes [121], and thus the authors suggested possible roles of MALAT1 and TUG1 in obesity.Moreover, Rasaei et al. [122] suggested that there may be a positive interaction effect between MALAT1 transcription levels and the cholesterol/saturated fat index, which impacts the visceral adiposity index and body adiposity index among overweight and obese women.However, it was found that MALAT1 expression was significantly reduced in atherosclerotic plaques.Furthermore, MALAT1 inhibition significantly reduced the mRNA of APOE and ABCA1 and increased ox-LDL uptake, lipid accumulation, and total cholesterol in macrophages.The authors also suggested that MALAT1 may promote cholesterol accumulation by regulating the miR-17-5p/ABCA1 axis in ox-LDL-induced THP-1 macrophages [123].
Aging leads to dysregulation and partitioning of fat stores as well as insulin resistance, but the exact mechanisms involved in these phenomena remain unknown.MALAT1 has been shown to be a gene that is strongly downregulated with aging, which may be due to a lower transcription rate and/or increased RNA instability during aging.Carter et al. [117] studied MALAT1 RNA as a potent gene that is downregulated in vWAT during normal aging in male mice.In female mice, reduced levels of MALAT1 in subcutaneous WAT (scWAT) were attributed to aging.In contrast, in males, a significant reduction in MALAT1 expression levels in vWAT, but not scWAT, was observed with age.The effects of MALAT1 on the size and number of adipocytes in WAT depots were investigated because MALAT1 has been linked to the transition of the proliferation/differentiation state.The researchers detected that in male mice, similar levels of MALAT1 were expressed in adipocytes and the stromal vascular fraction (SVF) in vWAT and scWAT.In vWAT of male mice, an age-related decrease in MALAT1 levels was observed in adipocytes, but not in SVF.Moreover, reduced MALAT1 expression in scWAT was also observed in genes (ob and db), as well as dietinduced obesity models.Based on these findings regarding MALAT1+/+ and MALAT1−/−, Carter et al. [117] studied mice from a single litter to determine whether loss of MALAT1 would affect age-or diet-induced fat mass gain and the development of glucose intolerance.The study found that Malat1-deficient males and females gained the same amount of weight and developed insulin resistance to a similar degree as MALAT1+/+ mice.Mice from one litter were divided into two groups: one that received regular food and one that had a high-fat, sucrose-rich diet.The researchers observed no clear differences in oxygen consumption, food intake, or lipid profiles between MALAT+/+ and MALAT1−/− mice, which indicates that the lack of MALAT1 does not impair or accelerate age-induced fat gain and insulin resistance.
Moreover, MALAT1 is responsible for fat accumulation in various organs.Its transcript has been shown to regulate lipid accumulation in the liver by increasing the stability of the sterol regulatory element-binding protein (SREBP)-1c [124]; this protein preferentially enhances the transcription of genes necessary for fatty acid synthesis.This thesis was supported by a study reported by Yan et al. [7], in which MALAT1 levels were found to be increased in hepatic HepG2 cells and primary mouse hepatocytes treated with palmitate.Under palmitate treatment, the increase in MALAT1 expression coincided with an increase in SREBP-1c in liver cells.The study showed that MALAT1 knockout in mice significantly reduced liver lipid levels in vivo, while MALAT1 overexpression in palmitatetreated HepG2 cells increased lipid accumulation.The study showed that excess palmitate increased MALAT1 lncRNA expression, activated SREBP-1c, and induced intracellular lipid accumulation in hepatocytes.MALAT1 expression was increased in hepatocytes exposed to palmitate and livers of ob/ob mice.The increased expression of SREBP-1c effectively abolishes the increase in intracellular triglyceride and cholesterol levels induced by MALAT1.This finding indicates that the effect of MALAT1 on intracellular lipid accumulation depends on SREBP-1c.This supports the thesis that MALAT1 plays a role in hepatic steatosis and insulin resistance.In conclusion, MALAT1 induces hepatic lipid accumulation and insulin resistance by increasing the expression of SREBP-1c and target genes.This study suggests that MALAT1 inhibition may have potential in the treatment of obesity and type 2 diabetes.
To build on our previous study [23], in which MALAT1 was positively correlated with fat accumulation in the subcutaneous fat tissue of a native fatty Polish pig breed, further work is needed to establish the role that this lncRNA plays during this process in pigs, whether it is in adipogenesis or the proliferation process of primary adipose cells.However, the evidence presented in this review highlights a wide range of MALAT1 functions in the generation of fat tissue, and we see a gap in the studies of MALAT1 using other lab animals.

Future Perspective
MALAT1 is a lncRNA that is expressed in multiple tissues and plays a significant role in critical molecular processes associated with the cell cycle, cancer, or hypoxia.Moreover, the evidence suggests that it is involved in numerous pathophysiological processes associated with many disorders or diseases in vascular or neurological systems and in cancer biology.Though MALAT1 executes its function during gene expression in the alternative splicing and transcriptional and post-transcriptional regulation of many genes, its simple silencing or overexpression seem to be promising as a target for disease contradiction.However, MALAT1 is expressed in almost all human tissues and has physiological functions in cells that are non-associated with pathological states.Therefore, it seems more effective to aim to limit RNA-protein interactions, which play a significant role during, for example, cancer progression.On the other hand, MALAT1 is expressed as multiple isoforms dependent on the tissue, and these isoforms often have specific functions.Therefore, the deepening of research in the context of MALAT1 isoforms may reveal a therapeutic target against cancer.
On the other hand, obesity is treated as a civilization disease, and we are still searching for gene targets for drugs that can be used to prevent this disorder.Currently, investigating individual genotypes makes it possible to estimate the predisposition to obesity occurrence and, based on this information and nutrigenetics, adjust the appropriate diet to individuals.Therefore, research should also focus on this aspect of MALAT1, searching of genetic markers association with regulation of adipogenesis and proliferation of primary fat cells, a mechanism related to fat deposition.Additionally, extending our knowledge of the new functions of lncRNAs delivers new possibilities for testing these interesting molecules.
As a final suggestion, farm animals are successfully used as study models in investigations aiming to find molecular mechanisms associated with fat deposition; therefore, the role of MALAT1 in this context may be examined using a different model than humans or small lab animals.Based on our experience, we recommend pigs as an animal model.

Figure 2 .
Figure 2. Role of MALAT1 lncRNAs in fat tissue generation and deposition.Illustration was prepared in Biorender.

Figure 2 .
Figure 2. Role of MALAT1 lncRNAs in fat tissue generation and deposition.Illustration was prepared in Biorender.