Network Pharmacology Prediction and Molecular Docking-Based Strategy to Explore the Potential Mechanism of Gualou Xiebai Banxia Decoction against Myocardial Infarction

The aim of this study was to investigate targets through which Gualou Xiebai Banxia decoction aids in treating myocardial infarction (MI) using network pharmacology in combination with molecular docking. The principal active ingredients of Gualou Xiebai Banxia decoction were identified from the TCMSP database using the criteria of drug-likeness ≥30% and oral bioavailability ≥0.18. Interactions and pathway enrichment were investigated using protein–protein interaction (PPI) networks and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, respectively. Active component structures were docked with those of potential protein targets using AutoDock molecular docking relative softwares. HIF1A was of particular interest as it was identified by the PPI network, GO and KEGG pathway enrichment analyses. In conclusion, the use of network pharmacology prediction and molecular docking assessments provides further information on the active components and mechanisms of action Gualou Xiebai Banxia decoction.


Introduction
Myocardial infarction (MI) results from arterial plaque accumulation leading to reductions in blood flow, reduced oxygen and damage to the heart muscles [1][2][3].MI symptoms include chest pain, heartbeat abnormalities, shortness of breath and nausea, amongst others [2,4].If these symptoms are not treated rapidly, MI can lead to death [5,6].It is thus important to prevent and alleviate the symptoms of MI to save patients' lives.
Traditional Chinese medicine (TCM) has been used for many thousands of years in China [7].The Gualou Xiebai Banxia decoction is an herbal formula that was first described in the "Synopsis of the Golden Chamber" by Zhang Zhongjing in the Eastern Han Dynasty [8].It is composed mostly of Gualou, Xiebai and Banxia [9].For centuries, Gualou and Xiebai have been used for the clinical treatment of cardiovascular diseases (CVDs), including MI, heart failure and arrhythmia [10,11].Extracts of Gualou (Trichosanthes kirilowii Maxim) and Xiebai (Allium macrostemon Bge) have been found to improve cardiac function and hemodynamics and have been shown to be effective in treating ischemia/reperfusion injuries and MI [12][13][14].Banxia (Pinellia pinellia), likewise, has significant therapeutic actions in CVD [14].The Gualou Xiebai Banxia decoction is a commonly prescribed TCM for the treatment of CVD, including MI [15].Furthermore, Gualou Xiebai Banxia decoction has been found to protect against type II diabetes (T2DM) treatment with acute myocardial ischemia (AMI) (T2DM-AMI) by attenuating oxidative stress (OS) and apoptosis via phosphatidyl inositol 3-kinase (PI3K)/serine/threonine protein kinase B (Akt)/endothelial nitric oxide synthase (e-NOS) signaling [15].Specifically, Gualou Xiebai Banxia decoction is reported to upregulate PI3K, Akt and e-NOS mRNA and protein in T2DM-AMI rats, leading to protection against T2DM-AMI [15].Although the Gualou Xiebai Banxia decoction Genes 2024, 15, 392 2 of 18 has been demonstrated to have a specific protective effect against MI, its mechanism of action and the pathways involved still require further study.
Network pharmacology was introduced by Hopkins in 2007 [16,17] and predicts drugtarget interactions based on the structures of the interacting molecules and the effectiveness of the drug's action.This approach takes into account the multiple interactions occurring within the body and constructs a network between the drug, the molecular target and the disease, together with an analysis of the genes associated with the disease [18].An understanding of the signaling and regulatory pathways involved in drug action can both improve the efficacy of the drug and reduce its side effects, thus ultimately improving the success of clinical trials and saving costs [19].Network pharmacology uses multiple levels of analysis, including omics datasets and in silico simulations, to analyze the mechanisms underlying drug effects.It is, thus, an ideal approach for analyzing the complexities of TCM [20].It is worth mentioning the relevance of molecular docking to drug research as it simulates the recognition process between molecules and predicts both the binding mode and the strength of the interaction between molecules and receptors through a scoring function.In recent years, molecular docking has contributed significantly to the field of computer-aided medical research.
Here, we used network pharmacology to explore the main components and molecular biological mechanisms of Gualou Xiebai Banxia decoction for the treatment of MI.Molecular docking was then used to verify the predicted interactions between the identified active components of Gualou Xiebai Banxia decoction and their molecular targets.The combination of molecular docking and network pharmacology provides a means of effectiveness for studying the action of Gualou Xiebai Banxia decoction, as well as a reference for follow-up research.Figure 1 shows a flow chart of the study.T2DM-AMI rats, leading to protection against T2DM-AMI [15].Although the Gualou Xiebai Banxia decoction has been demonstrated to have a specific protective effect against MI, its mechanism of action and the pathways involved still require further study.Network pharmacology was introduced by Hopkins in 2007 [16,17] and predicts drug-target interactions based on the structures of the interacting molecules and the effectiveness of the drug's action.This approach takes into account the multiple interactions occurring within the body and constructs a network between the drug, the molecular target and the disease, together with an analysis of the genes associated with the disease [18].An understanding of the signaling and regulatory pathways involved in drug action can both improve the efficacy of the drug and reduce its side effects, thus ultimately improving the success of clinical trials and saving costs [19].Network pharmacology uses multiple levels of analysis, including omics datasets and in silico simulations, to analyze the mechanisms underlying drug effects.It is, thus, an ideal approach for analyzing the complexities of TCM [20].It is worth mentioning the relevance of molecular docking to drug research as it simulates the recognition process between molecules and predicts both the binding mode and the strength of the interaction between molecules and receptors through a scoring function.In recent years, molecular docking has contributed significantly to the field of computer-aided medical research.
Here, we used network pharmacology to explore the main components and molecular biological mechanisms of Gualou Xiebai Banxia decoction for the treatment of MI.Molecular docking was then used to verify the predicted interactions between the identified active components of Gualou Xiebai Banxia decoction and their molecular targets.The combination of molecular docking and network pharmacology provides a means of effectiveness for studying the action of Gualou Xiebai Banxia decoction, as well as a reference for follow-up research.Figure 1 shows a flow chart of the study.

Identification of the Active Components of Gualou Xiebai Banxia Decoction and Their Target Genes
The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) (TCMSP) (https://tcmsp-e.com/tcmsp.php,accessed on 1 January 2023) is a unique tool that can be used to investigate the active components and target genes of TCM [21].Because the Gualou Xiebai Banxia decoction is composed mostly of "gualou", "xiebai" and "banxia", the TCMSP database was searched using the search terms "gualou", "xiebai" or "banxia" followed by the screening criteria of oral bioavailability (OB) ≥30% and drug-likeness (DL) ≥0.18 [22,23].The filtered results led to the compilation of the "gualou_ingredients.txt", "xiebai_ingredients.txt" and "banxia_ingredients.txt" files.Similarly, using the "Related targets" column, we obtained three tables of TCM components and targets, leading to the complilation of the "gualou_targets.txt","xiebai_targets.txt"and "banxia_targets.txt"files.The files were then sorted and merged using Perl language to form an "allTargets.txt"file that included information on both ingredients and targets.
To incorporate the TCM ingredients and gene symbols in one table, the potential protein targets and gene abbreviations (gene symbols) were investigated using the UniProt database (https://www.uniprot.org/,accessed on 1 January 2023).Here, we chose "human" as the "popular organism" and "reviewed" under "filter by".The filtered table was downloaded and copied into a single ann.txt file.This file was then integrated with the "allTargets.txt"file using Perl, resulting in the "allTargets symbol.txt"file.

Identification of Potential MI-Related Targets
The search term "myocardial infarction" was used in the OMIM (https://www.OMIM.org/, accessed on 1 January 2023), GeneCards (https://www.genecards.org/,accessed on 1 January 2023), DrugBank (https://www.DrugBank.ca,accessed on 1 January 2023) and PharmGkb (https://www.PharmGkb.org/,accessed on 1 January 2023) databases.In the GeneCards database, we selected potential targets with "Relevance Scores" greater than or equal to 1 for further investigation.The results from the four databases were compiled into a new.txtfile using R, resulting in a disease-related Venn diagram and a "disease.txt"file.In this process, potential targets were identified aft access er merging the search results and deleting duplicates.

Identification of Intersections between Drug Targets and Disease-Related Genes
To evaluate the intersections between the drug targets and disease-related genes, the Venny 2.1 (http://bioinfogp.cnb.csic.es/tools/venny/index.html,accessed on 1 January 2023) online tool was used for Venn diagram construction to determine the overlap between the active components and disease targets and thus to identify common genes for further analysis.

Construction of the Regulatory Network
For the construction of the Gualou Xiebai Banxia decoction regulatory network, the "allTargets symbol.txt"and "disease.txt"files were placed in one folder.The "net.geneLists.txt","net.molLists.txt","net.network.txt"and "net.type.txt"files were then obtained using Perl and "net.network.txt"and "net.type.txt"were used for molecular docking.Cytoscape 3.8.0 was then used to integrate the identified active components of the Gualou Xiebai Banxia decoction and its MI targets into a unified network of predicted interactions [24].In the network, components and genes are linked by lines [25].Specifically, the "net.network.txt"and "net.type.txt"files were imported into Cytoscape 3.8.0; the gene nodes were selected through "net.genelists.txt" and sorted by "degree".The layout of the nodes was then transformed to a grid and the size of the nodes was defined.After the selection of the drug component through "net.mollists.txt",it was defined as a ring.The different ingredients of Gualou Xiebai Banxia decoction were colored differently, with purple representing "gualou", "xiebai" colored green and "banxia" colored red.

Protein-Protein Interaction (PPI) Network Construction and Core Gene Screening
PPI networks are useful for understanding the functions of specific components and their links to disease and drug effects [26].Networks were constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database (https://STRING-db.org/,accessed on 1 January 2023) [27].A confidence level of 0.9 was set to hide individual nodes in the network and the results were saved in TSV format and imported to Cytoscape 3.8.0 for topological analysis and the identification of the hub genes in the network.Specifically, the protein interaction network was first imported into Cytoscape 3.8.0 and the score of each node was calculated by CytoNCA.Nodes were filtered according to the score and nodes with higher scores were selected to rebuild the network and obtain the network core.In the case of multiple network nodes, the network was re-scored to obtain more core hub genes.

Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Enrichment Analyses
GO enrichment analysis was performed to examine the functions of the genes in the regulatory network and in which functions they were enriched.To explore the pathways in which the identified genes were involved, KEGG enrichment analysis was performed.The names of the target genes were transformed into Entrez IDs using R [28].GO and KEGG analyses were conducted in R using cutoff values of False Discovery Rate (FDR) q < 0.05, followed by the selection and graphing of the top 30 items [29].They used the same packages, which are "colorspace", "stringi", "ggplot2", "BiocManager", "DOSE", "clusterProfiler" and "enrichplot".KEGG Mapper (https://www.kegg.jp/kegg/mapper/,accessed on 1 January 2023) was used for identifying pathways in which the identified genes were enriched [30].

Molecular Docking
Molecular docking is widely used in structure-based drug discovery [31,32].The small-molecule ligand and protein receptor files were first prepared.The 2D structures of the TCM components were obtained from PubChem (https://pubchem.ncbi.nlm.nih.gov/,accessed on 1 January 2023) and were converted to 3D structures using ChemOffice.These 3D structures were termed "lig.mol2".Structures of the hub proteins were downloaded from the Protein Data Bank (http://www.rcsb.org/,accessed on 1 January 2023) and PyMol was used to remove small ligands and water molecules.The structures were then exported and named "rep.pdb".The "lig.mol2" and "rep.pdb"files were then converted into ".pdbqt" format and the active pockets were identified.Specifically, AutoDockTools was used to hydrogenate receptors and convert ligands to .pbdqtformat.The x, y and z dimensions of the active pockets were defined as "40, 40, 40", respectively, and these parameters were saved.The grid was then exported and saved under the name "grid.gpf".Finally, docking was performed with AutoDock Vina, using receptor-ligand combinations with the lowest free energy.

Identification of Active Components of Gualou Xiebai Banxia Decoction
A total of 283 active components were initially identified in the Gualou Xiebai Banxia decoction using the TCMSP database.Of these, 248 components that were unrelated to MI were removed, resulting in 35 components for further analysis; these components are shown in Table 1.

Construction of an Active Component Library and MI Targets
We used the UniProt database to integrate the information on TCM ingredients and gene symbols in one table and the GeneCards, OMIM, DrugBank and PharmGkb databases to obtain the MI-related targets.This resulted in the identification of 2889 MI-related gene targets.Of these, 1641 were mapped with 207 compound targets, resulting in 97 common target genes (Table 2 and Figures 2 and 3).

Interactive Network of Targets
PPI networks assist in the exploration of interactions between proteins.In all networks, 97 genes were imported to STRING for PPI network construction and Cytoscape 3.8.0 was used for the identification of the hub target genes and an analysis of the PPI network results (Figure 4).CytoNCA was used to calculate the topology parameters of the nodes using betweenness centrality (BC), closeness centrality (CC), degree centrality (DC), eigenvector centrality (EC), network centrality (NC) and local average connectivity (LAC).The threshold values applied during the first screening were BC > 4.472, CC > 0.072, DC > 3.000, EC > 0.032, NC > 2 and LAC > 1, resulting in 17 nodes and 70 edges.Obviously, this result contained too many nodes and edges and further screening was necessary.The second screening threshold was BC > 6.472, CC > 0.667, DC > 8.000, EC > 0.227, NC > 6.464 and LAC > 4.545, resulting in seven nodes and 21 edges.These seven nodes included HIF1A, MYC, TP53, MAK1, MAK3, ESR1 and RELA (Figure 5).Table 3 shows that these targets have the same "degree".

Interactive Network of Targets
PPI networks assist in the exploration of interactions between proteins.In all networks, 97 genes were imported to STRING for PPI network construction and Cytoscape 3.8.0 was used for the identification of the hub target genes and an analysis of the PPI  Intersection of drug targets and disease-related genes.The active ingredients refer to "Guolou", "Xiebai" and "Banxia".

Interactive Network of Targets
PPI networks assist in the exploration of interactions between proteins.In all networks, 97 genes were imported to STRING for PPI network construction and Cytoscape 3.8.0 was used for the identification of the hub target genes and an analysis of the PPI 3.000, EC > 0.032, NC > 2 and LAC > 1, resulting in 17 nodes and 70 edges.Obviously, this result contained too many nodes and edges and further screening was necessary.The second screening threshold was BC > 6.472, CC > 0.667, DC > 8.000, EC > 0.227, NC > 6.464 and LAC > 4.545, resulting in seven nodes and 21 edges.These seven nodes included HIF1A, MYC, TP53, MAK1, MAK3, ESR1 and RELA (Figure 5).Table 3 shows that these targets have the same "degree".Here, the purple color stands for "Gualou", the green color for "Xiebai" and the red color for "Banxia", respectively.Here, the purple color stands for "Gualou", the green color for "Xiebai" and the red color for "Banxia", respectively.

Regulatory Network of Active Components
The regulatory network of the TCM compounds was constructed to determine the relationships between the active components and the target genes.The predicted targets of Gualou Xiebai Banxia decoction and the corresponding active components were used to establish a network that was imported into Cytoscape 3.8.0.The network had 123 nodes (26 active components and 97 target genes) (Figure 5).In the figure, the circles on the left represent the active components and the rectangles on the right are the gene targets.Node sizes were determined by the number of associated active components.It can be clearly seen that the Gualou Xiebai Banxia decoction components may target multiple proteins and many TCM ingredients may contain the same monomer compound.

GO and KEGG Pathway Enrichment Analyses
GO enrichment analysis was conducted to further examine the functions of the intersecting genes.This identified 1767 biological processes (BPs), including "hypoxia reaction", "drug reaction" and "cell response to lipopolysaccharide".In the cell components (CC) category, it was found that the proteins were mainly located in the cell membrane.At the same time, the molecular function (MF) category mainly included "binding protein", "intrinsic protein binding", "enzyme" and "protein homogenization activity", amongst others (Figure 6).As shown in Figure 6, gene functions were classified into the GO categories of biological process, cell component and molecular function, which is related to the response to metal ion, response to hypoxia and other biological process functions.This means that Gualou Xiebai Banxia decoction may treat MI by influencing these biological processes, such as response to oxygen levels.
KEGG enrichment analyses of the Gualou Xiebai Banxia decoction target genes were performed using R language.Figure 6 shows the first 30 signal pathways, which involve the PI3K-AKT signaling pathway, IL-17 signaling pathway and TNF signaling pathway and so on.This means that Gualou Xiebai Banxia decoction may treat MI by influencing these pathways, such as the HIF-1 signaling pathway.
It is worth noting that the results of the GO and KEGG analyses were obtained by running the R language and code in conjunction with the Drug_Disease.txtfile (Drug_Disease.txt).Interestingly, an analysis of the GO and KEGG results showed that the HIF-1 signaling pathway ranked at the top of the histogram, suggesting that this pathway plays a significant role in MI treatment with the Gualou Xiebai Banxia decoction.

Target Pathway Analysis
KEGG Mapper was used for the identification of pathway maps, as seen in Figure 7, where the hub targets are colored blue.The identified pathways included the "PI3K signaling pathway", "Jak signaling pathway", "HIF-1 signaling pathway" and "mTOR signaling pathway".As seen in the figure, the hub target genes are mainly involved in cell differentiation and apoptosis, suggesting that the Gualou Xiebai Banxia decoction may regulate several processes and these processes may include specific hub target proteins.

Target Pathway Analysis
KEGG Mapper was used for the identification of pathway maps, as seen in Figure 7, where the hub targets are colored blue.The identified pathways included the "PI3K signaling pathway", "Jak signaling pathway", "HIF-1 signaling pathway" and "mTOR signaling pathway".As seen in the figure, the hub target genes are mainly involved in cell differentiation and apoptosis, suggesting that the Gualou Xiebai Banxia decoction may regulate several processes and these processes may include specific hub target proteins.

Molecular Docking
Based on the network pharmacology results file (net.network.txt)and the first seven identified protein targets, we identified four compounds, baicalein, quercetin, naringenin and aconitine, which were docked to the first seven identified protein targets.Ligandreceptor docking indicated that the active compounds in the Gualou Xiebai Banxia decoction were likely to interact with the hub proteins and may influence MI treatment through the regulation of HIF1A, MYC, TP53, MAK1, MAPK3, ESR1 and RELA.Details of the four top compounds are shown in Table 4. Table 5 indicates that all the key compounds in the Gualou Xiebai Banxia decoction had a strong affinity for these proteins.The molecular docking results are shown in Figure 8, demonstrating that the active components of Gualou Xiebai Banxia decoction bind to the amino acid residues of hub proteins through hydrogen bonds; for example, quercetin interacts with HIF1A through Arg17, Val264 and Thr39.The lengths of the hydrogen bonds are also shown in Figure 8.Thus, the molecular docking results verified the network pharmacology findings.

Molecular Docking
Based on the network pharmacology results file (net.network.txt)and the first seven identified protein targets, we identified four compounds, baicalein, quercetin, naringenin and aconitine, which were docked to the first seven identified protein targets.Ligandreceptor docking indicated that the active compounds in the Gualou Xiebai Banxia decoction were likely to interact with the hub proteins and may influence MI treatment through the regulation of HIF1A, MYC, TP53, MAK1, MAPK3, ESR1 and RELA.Details of the four top compounds are shown in Table 4. Table 5 indicates that all the key compounds in the Gualou Xiebai Banxia decoction had a strong affinity for these proteins.The molecular docking results are shown in Figure 8, demonstrating that the active components of Gualou Xiebai Banxia decoction bind to the amino acid residues of hub proteins through hydrogen bonds; for example, quercetin interacts with HIF1A through Arg17, Val264 and Thr39.The lengths of the hydrogen bonds are also shown in Figure 8.Thus, the molecular docking results verified the network pharmacology findings.

Molecular Docking
Based on the network pharmacology results file (net.network.txt)and the first seven identified protein targets, we identified four compounds, baicalein, quercetin, naringenin and aconitine, which were docked to the first seven identified protein targets.Ligandreceptor docking indicated that the active compounds in the Gualou Xiebai Banxia decoction were likely to interact with the hub proteins and may influence MI treatment through the regulation of HIF1A, MYC, TP53, MAK1, MAPK3, ESR1 and RELA.Details of the four top compounds are shown in Table 4. Table 5 indicates that all the key compounds in the Gualou Xiebai Banxia decoction had a strong affinity for these proteins.The molecular docking results are shown in Figure 8, demonstrating that the active components of Gualou Xiebai Banxia decoction bind to the amino acid residues of hub proteins through hydrogen bonds; for example, quercetin interacts with HIF1A through Arg17, Val264 and Thr39.The lengths of the hydrogen bonds are also shown in Figure 8.Thus, the molecular docking results verified the network pharmacology findings.

Target Pathway Analysis
KEGG Mapper was used for the identification of pathway maps, as seen in Figure 7, where the hub targets are colored blue.The identified pathways included the "PI3K signaling pathway", "Jak signaling pathway", "HIF-1 signaling pathway" and "mTOR signaling pathway".As seen in the figure, the hub target genes are mainly involved in cell differentiation and apoptosis, suggesting that the Gualou Xiebai Banxia decoction may regulate several processes and these processes may include specific hub target proteins.

Discussion
Network pharmacology utilizes resemblances between the structures and the documented effects of known drugs and ligands to construct a network of interactions between ligands and targets [33].This method takes crosstalk between signaling pathways into account, allowing the prediction of multiple interactions that may be used in rational drug design [34][35][36].
Here, we investigated the mechanism of Gualou Xiebai Banxia decoction in treating MI using various techniques, including network pharmacology, pathways analysis and molecular docking between the identified active components and predicted targets.The analysis identified 97 potential target proteins, with PPI network analysis narrowing these down to seven final targets, specifically, HIF1A, MYC, MAPK3, MAPK1, TP53, ESR1 and RELA.Molecular docking (Figure 8) showed that four active components of the decoction, namely, baicalein, quercetin, naringenin and coniferin, can interact directly with these target proteins.Many studies have demonstrated interactions between these active compounds and their targets [37][38][39].For example, Chao et al. demonstrated that baicalein protects against retinal ischemia through anti-apoptotic effects, as well as through the downregulation of HIF-1α, vascular endothelial growth factor (VEGF) and MMP-9 and upregulation of HO-1 [36].Our study illustrates that quercetin also interacts with the targets identified here.
GO analysis calculates the hypergeometric distribution relationship to a particular branch of the GO classification, based on the common genes.This analysis is helpful in identifying protein functions and in pharmacological studies [39].GO annotates genes according to function, while KEGG analysis considers the physiological pathways [40].The integration of these two procedures allows the identification of specific genes and pathways [41].KEGG enrichment analysis indicated that the HIF-related pathway, inflammation-related pathways and PI3K/AKT-related pathways may be involved in the treatment of MI by Gualou Xiebai Banxia decoction.Interestingly, RELA, which forms part of the NF-κB pathway, also regulates the inflammatory pathway.In addition, from Figure 8, we can see that the PI3K/AKT pathway indirectly interacts with TP53 and affects the expression of TP53.The MAPK pathway is related to MYC and ESR proteins, while the VEGF signaling identified by KEGG may affect the MAPK pathway [42].Through the analysis of GO and KEGG, we found that HIF1A, MYC, MAPK, TP53, ESR and RELA were likely targets of the Gualou Xiebai Banxia decoction in treating MI.Importantly, the GO, KEGG and PPI analyses all showed that HIF1A was the most significant of the identified target proteins, suggesting its importance in the action of the Gualou Xiebai Banxia decoction in the prevention or treatment of MI.However, to date, there have been no studies investigating the effects of Gualou Xiebai Banxia decoction on HIF1A, suggesting the usefulness of further investigation of this protein as a novel target of Gualou Xiebai Banxia decoction in cell and animal experiments.In addition, ESR, as one of the main targets, provides a new direction for the study of the mechanism of Gualou Xiebai Banxia decoction.It is worth noting that we have not focused on any sub-datasets for KEGG enrichment analysis, as our previous results have been screened for seven important targets, which we focus on most in our current analysis.However, KEGG results can be used as a reference for further study to demonstrate that these targets or other targets are critical and involved in MI process.
The main purpose of this research was to use network pharmacology prediction and a molecular docking-based strategy to explore the potential mechanism of Gualou Xiebai Banxia decoction in the treatment and prevention of MI.The combination of molecular docking and network pharmacology provides an effective means of studying the effects of Gualou Xiebai Banxia decoction to provide a reference for follow-up research.However, there are some limitations to the present study.It would be worthwhile to conduct additional animal or clinical experiments to verify the results.Issues such as the TCM dosage, safety and its effects after administration also require further investigation.
In this paper, we used AutoDock Vina for molecular docking, which has a relatively high average accuracy of binding mode prediction and easy operation.It successfully simulates the binding of large-molecule proteins and small-molecule ligands.We focused on network pharmacology combined with molecular docking.Network pharmacology emphasizes holistic systems and biological networks when analyzing the laws of molecular association between drugs and therapeutic targets.By focusing on drug research, it allows for an analysis of the complexity of TCM formulations, together with an explanation of the overall mechanisms of action and the provision of new scientific and technological support for drug development.With the increasing influence and applications of the big data era, network pharmacology faces both significant development opportunities and challenges [43].There are still many problems in current network pharmacology research, such as uneven study quality, a lack of standardized data and insufficient scientific validation.Moreover, some of the predicted targets and pathways may have undesirable effects on the drug safety, such as dizziness, headache and nitrate-related tolerance [44].Therefore, the optimal dose and duration of the compound require further investigation before clinical application.Thus, the establishment of rigorous, scientific and uniform criteria for the evaluation of network pharmacology is urgently needed for the further development of network pharmacology research [43].
To summarize, Gualou Xiebai Banxia decoction contains many active components that interact with specific targets to influence downstream pathways, leading to the treatment of MI.The results showed that baicalein, quercetin, naringenin and coniferin were the key active compounds in Gualou Xiebai Banxia decoction and suggested that they may play significant roles in MI treatment.In addition, HIF1A, MYC, MAPK3, MAPK1, TP53, ESR1 and RELA were identified as key target proteins and, of these, HIF1A was the most significant.This article is the first to systematically explore the possible targets and mechanisms of Gualou Xiebai Banxia decoction in the treatment of MI.It provides a new theoretical basis for exploring therapeutic drugs and their mechanisms for the treatment of MI.However, the precise roles and mechanisms of action of the active components of Gualou Xiebai Banxia decoction in the treatment of MI have not been fully explained and verified, suggesting directions for future research.

Figure 1 .
Figure 1.A flow chart depicting the whole framework based on network pharmacology and molecular docking.

Figure 1 .
Figure 1.A flow chart depicting the whole framework based on network pharmacology and molecular docking.

Figure 3 .
Figure 3. Venn diagram of active ingredients and disease targets.Intersection of drug targets and disease-related genes.The active ingredients refer to "Guolou", "Xiebai" and "Banxia".

Figure 3 .
Figure 3. Venn diagram of active ingredients and disease targets.Intersection of drug targets and disease-related genes.The active ingredients refer to "Guolou", "Xiebai" and "Banxia".

Figure 3 .
Figure 3. Venn diagram of active ingredients and disease targets.Intersection of drug targets and disease-related genes.The active ingredients refer to "Guolou", "Xiebai" and "Banxia".

Figure 5 .
Figure 5. Identification of the PPI network core using Cytoscape 3.8.0.The score of each node is calculated using the cytoscape plug-in (CytoNCA) after selecting a node with a higher score and rebuilding the network to obtain the network core.

Figure 5 .
Figure 5. Identification of the PPI network core using Cytoscape 3.8.0.The score of each node is calculated using the cytoscape plug-in (CytoNCA) after selecting a node with a higher score and rebuilding the network to obtain the network core.

Figure 5 .
Figure 5. Identification of the PPI network core using Cytoscape 3.8.0.The score of each node is calculated using the cytoscape plug-in (CytoNCA) after selecting a node with a higher score and rebuilding the network to obtain the network core.

Figure 6 .
Figure 6.Enriched GO terms and KEGG pathways for 97 target genes.

Figure 6 .
Figure 6.Enriched GO terms and KEGG pathways for 97 target genes.

Figure 7 .
Figure 7. Pathway map of Gualou Xiebai Banxia decoction for MI treatment.Solid arrows indicate activation effects, T arrows indicate inhibition effects and segments indicate activation or inhibition effects.The protein in blue solid box is the pivotal gene of Gualou Xiebai Banxia decoction for MI treatment.

Figure 7 .
Figure 7. Pathway map of Gualou Xiebai Banxia decoction for MI treatment.Solid arrows indicate activation effects, T arrows indicate inhibition effects and segments indicate activation or inhibition effects.The protein in blue solid box is the pivotal gene of Gualou Xiebai Banxia decoction for MI treatment.

Figure 7 .
Figure 7. Pathway map of Gualou Xiebai Banxia decoction for MI treatment.Solid arrows indicate activation effects, T arrows indicate inhibition effects and segments indicate activation or inhibition effects.The protein in blue solid box is the pivotal gene of Gualou Xiebai Banxia decoction for MI treatment.

Figure 8 .
Figure 8.Molecular docking of Gualou Xiebai Banxia decoction active ingredients and hub targets.In this figure, the black box is the active molecule of the drug and the enlarged figure is the combination of the molecule and the protein.These diagrams are predicted based on AutoDock Vina.

Table 2 .
The common targets of Gualou Xiebai Banxia decoction and MI.

Table 3 .
The centrality measures core targets of Gualou Xiebai Banxia decoction and MI.

Table 4 .
The basic information of the top four compounds.

Table 4 .
The basic information of the top four compounds.

Table 4 .
The basic information of the top four compounds.

Table 5 .
The binding energy of top four compounds.

Table 5 .
The binding energy of top four compounds.

Figure 6 .
Enriched GO terms and KEGG pathways for 97 target genes.

Table 5 .
The binding energy of top four compounds.