Genome-Wide Identification and Analysis of the WRKY Gene Family and Cold Stress Response in Acer truncatum

WRKY transcription factors constitute one of the largest gene families in plants and are involved in many biological processes, including growth and development, physiological metabolism, and the stress response. In earlier studies, the WRKY gene family of proteins has been extensively studied and analyzed in many plant species. However, information on WRKY transcription factors in Acer truncatum has not been reported. In this study, we conducted genome-wide identification and analysis of the WRKY gene family in A. truncatum, 54 WRKY genes were unevenly located on all 13 chromosomes of A. truncatum, the highest number was found in chromosomes 5. Phylogenetic relationships, gene structure, and conserved motif identification were constructed, and the results affirmed 54 AtruWRKY genes were divided into nine subgroup groups. Tissue species analysis of AtruWRKY genes revealed which were differently exhibited upregulation in flower, leaf, root, seed and stem, and the upregulation number were 23, 14, 34, 18, and 8, respectively. In addition, the WRKY genes expression in leaf under cold stress showed that more genes were significantly expressed under 0, 6 and 12 h cold stress. The results of this study provide a new insight the regulatory function of WRKY genes under abiotic and biotic stresses.


Introduction
Transcription factors are the most abundant gene regulators in multicellular genomes. They activate or inhibit the expression of target genes by binding to specific DNA sequences, thus regulating the gene expression of all organisms [1][2][3]. WRKY transcription factors are one of the largest and most important families of transcription factors with a highly conserved protein structure domain [4,5]. Structurally, approximately 60 amino acid residues of the N-terminus contain the conserved sequence associated with DNA binding activity (WRKYGQK), and the C-end have a zinc-finger motif (Cx 4-5 Cx 22-23 HxH or Cx 7 Cx 23 HxC) to participate in zinc finger protein interactions [6][7][8]. WRKY proteins are divided into three groups based on the number of conserved domain and type of zinc finger, named group I, group II and group III [9,10]. Group I comprises two Cx 4-5 Cx 22-23 HxH zinc figure motifs; group II contains one Cx 4-5 Cx 22-23 HxH zinc finger motif; and group III includes one Cx 7 Cx 23 HxC zinc finger motif [6]. On the basis of their phylogenetic clades and different assembling, the three groups can be further divided into subgroups, such as group II have five subgroups (II a-e) [11,12]. WRKY has many special biological functions due to its unique domain.
Numerous studies have indicated the crucial regulatory roles of WRKY transcription factors in plant growth and development, physiological and biochemistry processes, biotic and abiotic stress response [13][14][15][16]. For instance, overexpression of the transcription factor TaWRKY2 enhances drought stress tolerance and increase grain yield in transgenic wheat (Triticum aestivum L.) [17]; while overexpression of OsWRKY29 represses seed dormancy by directly downregulating the expression of OsABF1 and OsVP1 in rice (Oryza sativa L.) [18]. What is noteworthy is that the regulatory functions of WRKY genes are closely associated with multiple plant hormone-mediated signal pathways. In Arabidopsis thaliana, AtWRKY46 modulated the development of lateral roots in osmotic/salt stress conditions via regulation of ABA signaling and auxin homeostasis [4]. Cold tolerance is a response of plants to abiotic stress conditions, the gene expression levels of plants will change differently under cold stress. Many studies have proved that members of the WRKY family play a crucial regulatory role in cold stress, for instance, the expression of some PmWRKY genes was induced by cold stress in Prunus mume, which show 6 PmWRKY genes were downregulated, and three genes were upregulated expressed sustainably with prolonging of the treatment time in stems [19]. In Camellia sinensis, CsWRKY33, CsWRKY34, CsWRKY37, CsWRKY38 and CsWRKY39 genes were upregulated under low (4 • C) temperature treatments [20].
Acer truncatum, a deciduous tree belonging to the Aceraceae, is a crucial landscaping tree species with high ornamental and economic value [21,22]. The natural distribution range of A. truncatum is mainly concentrated in northern China, Korea and Japan, but there are a few sporadically distributed species in Europe and North America [23,24]. Due to its elite hardwood, it is widely used for timber production. Seeds and leaves are rich in oils, nervonic acid and tannins, which can also be used as raw materials for food processing and pharmaceutical development [25][26][27]. Therefore, increasing attention has been given to the various uses of A. truncatum, such as ornamental greening, medicinal value and ecological benefit, and research on A. truncatum has also increased. In recent years, research on the molecular biology aspects of A. truncatum has mainly focused on molecular markers, genetic diversity, and drug synthesis, but studies on its gene family are still lacking. Publication of the whole genome sequence of A. truncatum makes up for the gap in molecular biology research [28], which will be of great significance for further studies on A. truncatum flowering, seed production, and biological and abiotic stress.
In this study, we comprehensively analyzed the WRKY gene family with multiple bioinformatics methods and further determined the function of WRKY in growth and development. Furthermore, some AtruWRKYs were preliminarily verified in regulating A. truncatum tolerance to cold stress, and the transcript level of these responsive WRKY genes influence A. truncatum response to cold stresses. The results lay a theoretical foundation for deeper research on WRKY genes.

Plant Materials
The 3-year-old A. truncatum used in this study from the Northeast Forestry University greenhouse (126 • 38 8.92 E, 45 • 43 20.64 N), Harbin, Heilongjiang province, China. The A. truncatum seedlings was positioned in a low-temperature refrigerator at 4 • C to experimentally validate the computationally predicted AtruWRKY genes, and leaves of samples were collected at 6, 12, 24, 36 and 48 h after treatment with 0 h as a control. The collected leaves were instantly frozen in liquid nitrogen for 5 min and stored at −80 • C until they were used for extracting total RNA.

Sequence Retrieval of the WRKY Gene Family in A. truncatum
The A. truncatum genome files v1.1 (such as A._truncatum.gff, A._truncatum.pep and A._truncatum_genome.fa) was retrieved in https://doi.org/10.6084/m9.figshare.12986237 .v2, accessed on 8 July 2021 and used to identify AtruWRKY [28]. Next, 74 known WRKY transcription factor family genes from A. thaliana were selected as the query objects, and we obtained the Arabidopsis protein sequence by TAIR 9.0 release (https://www.arabidopsis. org/browse/genefamily/index.jsp, accessed on 10 July 2021) [29]. The protein sequences of A. truncatum and A. thaliana were subjected to BLAST alignment by TBtools (Toolbox for Biologists) v1.086 (evalue <1 × 10 −5 ) [30], each A. thaliana gene was successfully matched with multiple AtruWRKY genes (Table S1). Further, a total of 572 alignment sequence IDs were obtained after eliminating the repetition values and blanks. Then, candidate protein sequences were extracted by TBtools. In addition, the candidate AtruWRKY and A. truncatum were further manually analyzed using Batch CD-Search in the National Centre for Biotechnology database (NCBI; https://www.ncbi.nlm.nih.gov/Structure/bwrpsb/ bwrpsb.cgi, accessed on 11 July 2021) to confirm the presence of the WRKY domain. We estimated 7 conserved domains in A. truncatum: WRKY, UPF0242 superfamily, Plant zinc cluster domain (Plant_zn_clust, PF10533), PLN0321 superfamily, PKc-like superfamily, and PAH − and Sin3 superfamily. Finally, only the genes containing conserved WRKY domains were selected for subsequent analysis, and a total of 54 AtruWRKYs were obtained and termed AtruWRKY1 to AtruWRKY54 ( Table 1).
The proteomics and sequence analysis tools on the ExPASy (http://expasy.org/, accessed on 13 July 2021) proteomics server was used to predict the protein molecular weights (MW) and isoelectric points (PI) of AtruWRKY genes. To ensure the accuracy and completeness of the data, the CDS length and amino acid sequence were predicted by TBtools [31]. In addition, subcellular localization (https://wolfpsort.hgc.jp/, accessed on 13 July 2021) were calculated through the online website.

The Gene Structure and Chromosomal Location
To predict the exon-intron structure, the gene structure of all candidate A. truncatum WRKY genes was identified by TBtools (used A._truncatum.gff and genes ID), and TBtools software was used for visualization (from GTF/GFF3 File) [32,33]. At the same time, TBtools was used to determine the chromosomal location of the AtruWRKY genes, and their gene ID and sequences were used as the basic data for the searches. According to the gene location and number, each AtruWRKY gene was mapped to A. truncatum chromosomes, where tandemly duplicated gene pairs were linked with a red line [34,35]. It is well known that A. truncatum has 13 pairs of chromosomes [28], so they were named chr1, chr2, chr3, chr4, chr5, chr6, chr7, chr8, chr9, chr10, chr11, chr12 and chr13 in this study.

Protein Motif Composition Analysis and Functional Annotation
To identify the conserved motifs in A. truncatum proteins, the motifs within the 54 A. truncatum WRKY protein sequences were detected using the Multiple EM for Motif Elicitation (MEME 5.3.2: https://meme-suite.org/meme/doc/meme.html?man_type= web, accessed on 20 July 2021) [36]. The maximum number of motifs was set to 10, and members of the same subfamily have similar conserved domain characteristics. In addition, GO annotation was performed by Eggnog (http://eggnog5.embl.de/, accessed on 20 July 2021) and visualized using WEGO 2.0 (https://wego.genomics.cn/, accessed on 21 July 2021).

Sequence Alignment and Phylogenetic Tree Construction
According to the conserved domain of the A. truncatum WRKY protein, the family can be divided into different groups. The Clustalw algorithm analysis was performed, and the multiple sequence alignment analysis using the default parameters in MEGA6.0: 1000 replicates for bootstrap analysis and pairwise deletion [37,38]. Then, the phylogenetic tree was constructed from the alignment results using neighbor-joining (NJ) method. The phylogenetic tree was annotated and beautified by using iTOL online software (https://itol.embl.de/, accessed on 24 July 2021) [39,40]. Additionally, we downloaded the genomic information of A. thaliana (TAIR10.1; https://www.ncbi.nlm.nih.gov/genome/ ?term=+Arabidopsis+thaliana, accessed on 8 July 2021) and a related species called Acer yangbiense (AYv1.1; https://www.ncbi.nlm.nih.gov/genome/?term=Acer+yangbiense+, accessed on 29 July 2021) from the NCBI website and analyzed the collinearity with the information for the three species using TBtools [41].

Analysis of AtruWRKY Gene Expression in Different Tissues/Organs
To analyze the expression profile of AtruWRKY genes in different tissues/organs, RNAseq data of AtruWRKY genes were downloaded from the NCBI databases (https://www. ncbi.nlm.nih.gov/sra, accessed on 3 September 2021), which contains the expression levels in root (SRR10097461), leaf (SRR10097462), flower (SRR10097463), stem (SRR10097460) and seed (SRR10097465). The expression abundance of AtruWRKY genes were calculated using the fragments per kilobase of transcript per million fragments mapped (FPKM) values. R studio (pheatmap; https://cran.r-project.org/web/packages/pheatmap/index.html, accessed on 4 September 2021) was used for standardized analysis and visualization of gene expression data.

Real-Time qRT-PCR Experimental Validation
The OMEGA Biotek (Guangzhou Feiyang Biological Engineering Co., Ltd., Guangzhou, China) was used to extract total RNA from leaves (≤100 mg), and the experimental operations were carried out strictly according to the instructions (Version: Plant RNA Kit R6827; http://omegabiotek.com.cn/template/productShow.aspx?m=129002&i=100000 111413546, accessed on 10 September 2021). In addition, the RNA integrity and RNA purity (OD260/230 and OD260/280) of the samples were determined by agarose gel electrophoresis and ultrasonic spectrophotometry. To obtain the the amplification products of cDNA for qRT-PCR analysis, approximately 1 ug of total RNA was reverse-transcribed using the PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Kyoto, Japan). The primers were designed using the online website INTEGRATED DNA TECHNOLOGIES (https://sg.idtdna.com/pages, accessed on 22 September 2021), a total of 15 WRKY genes were selected randomly and used for RT-qPCR analysis with specific primers, and 18S rRNA was used as a reference gene (Table S2). The amplicon size ranging from 150 bp to 200 bp and an optimal Tm of 62 ± 1 • C. Quantitative real-time qRT-PCR was performed on an ABI 7500 Real-Time system (Applied Biosystems) using the TaKaRa SYBR Green Mix kit (TaKaRa, Beijing, China). The PCR protocol was conducted with a 20 µL volume, which contained 0.4 uL of ROX Reference Dye II, 0.8 uL of upstream and downstream primers (10 umol/L), 2 uL of cDNA template, 6 uL of double-distilled water (ddH 2 O) and 10 uL of 2 × SYBR (TB Green Premix Ex Taq II). The PCR reaction program consisting of 95 • C for 30 s, followed by 40 cycles of 95 • C for 5 s and 62 • C for 35 s, 95 • C for 15 s, and 60 • C for 1 min, finishing with 95 • C for 15 s. Three technical repetitions were performed for the whole experiment, the relative expression level was calculated using the 2 −∆∆CT method.

Identification of AtruWRKY in A. truncatum
Based on the amino acid sequences of the A. thaliana WRKY gene family, a total of 54 AtruWRKY genes were successfully identified from the A. truncatum genome by multiple sequence alignment after removing duplicates, incomplete sequences, and sequences without corresponding domains, and they were named AtruWRKY1 to AtruWRKY54 according to their Gene ID and structure. Detailed information on the 54 WRKY genes is provided in Table 1. The number of amino acids (aa) in the predicted protein varied from 71 to 768. The average predicted isoelectric points and molecular weight points of the encoded proteins were 7.01 and 42,388.03, respectively. The subcellular localization results showed that most of the WRKY genes (94%) were localized in the nucleus, and only the AtruWRKY1, AtruWRKY5 and AtruWRKY24 genes were localized in the cytosol.

Phylogenetic Tree Construction and Conserved Motifs
To further explore the phylogenetic relationship of the WRKY transcription factor family in A. truncatum, a phylogenetic tree of A. truncatum was constructed by using MEGA software as shown in Figure 1, which intuitively reflects the evolutionary status and grouping attribution of 54 members of the WRKY family. According to 54 WRKY protein sequences and clustering analysis, the 54 identified members of the WRKY family were sorted into three large groups. Specifically, the largest number of WRKY members in the third largest group was 29. The first and second groups had 24 and 11 members, respectively. Furthermore, a total of 10 motifs were identified by analyzing the conserved motifs of WRKY family members through online MEME. Based on the number and amino acid sequences of the conserved WRKY domains, we divided the three large groups into nine subgroups (I a, I b, II a, II b, III a, III b, III c, III d and III e). As shown in Figure 2, the same subgroups of WRKY members usually had similar motifs. For instance, almost all gene had more than 4 motifs, and all genes except AtruWRKY5 and AtruWRKY31 contained motif 2, which might play a crucial role in A. truncatum proteins. Interestingly, motif 5 was observed only in group I b, whereas motif 9 was only found in groups III d and III e. The group I b had the highest average number of motifs, approximately 8.

Chromosomal Location and Gene Structure
All 54 AtruWRKY genes were mapped to 13 chromosomes (2n = 2X = 26) in the A. truncatum genome (Figure 3). Notably, although WRKY genes were not evenly distributed on chromosomes, they were present on every chromosome. Chr5 contained the most AtruWRKY genes (9), the number of genes located on other chromosomes ranged from 1 to 6, and the lowest number of genes appeared on chr13 (1). There were six AtruWRKYs on chr6 and chr8 and only two AtruWRKYs on chr1 and chr3. Gene tandem duplication is considered to be an important reason for the formation of gene clusters [42,43]. In this study, we discovered that some AtruWRKYs were adjacent to each other. For example, AtruWRKY14 and AtruWRKY17 on chr7 were connected in series, suggesting that there may be a tandem duplication relationship between these AtruWRKYs. Additionally, we further investigated the WRKY exon-intron coding sequence structure to obtain in-depth insight into the protein expression sequence. The results showed that each group had a highly conserved structure (Figure 4). The distribution of CDS, UTR and introns of WRKY genes had a certain regularity, the intron number in the A. truncatum WRKY family ranged from 1 (AtruWRKY46) to 5 (AtruWRKY21), with an average number of 2.74, and group I genes contained more introns. The number of CDSs was evenly distributed in AtruWRKY genes, ranging from 3 to 4, but there was no UTR in many AtruWRKY (5,6,8,11,14,17,18,24,27,28,30,36,39,40,43,45,49,52) genes.

Synteny Analysis of AtruWRKY Genes
To reveal the origin and evolution of the A. truncatum WRKY family members, a synteny analysis was performed between WRKY genes in A. truncatum and two other plants, including A. thaliana and A. yangbiense ( Figure 5). Red lines in the background highlight syntenic WRKY gene pairs within A. truncatum and other plant genomes, while gray lines indicate collinear blocks. Fifty and 65 collinear gene pair showed syntenic relationships with those in the other two species: A. thaliana and A. yangbiense, respectively. A. truncatum and A. yangbiense are both members of Aceraceae, and WRKY genes show stronger homology than A. thaliana.

AtruWRKY Expression Profiles in Five Tissues
The expression levels of all 54 AtruWRKYs were investigated thoroughly using a rigorous transcriptome analysis procedure based on public transcriptomic data of different tissues of A. truncatum, including flowers, leaves, roots, seeds and stems. Among the 54 AtruWRKY genes, 52 AtruWRKYs (except AtruWRKY49 and AtruWRKY53) were identified in all tissue types ( Figure 6). The AtruWRKY genes showed different expression across tissues tested; one gene in the flower (AtruWRKY11) exhibited the highest transcript levels, and the expression of AtruWRKY34, AtruWRKY46 and AtruWRKY5 occurred preferentially in seeds. Six AtruWRKY genes (AtruWRKY24, AtruWRKY9, AtruWRKY8, AtruWRKY2, AtruWRKY18 and AtruWRKY30) showed higher expression levels in roots than in seeds and stems. The expression analysis of the different leaf developmental stages showed that several genes (AtruWRKY52, AtruWRKY15 and AtruWRKY17) had higher expression in the stem.

Functional Annotation
Of all the AtruWRKY genes identified, most (47 genes) were successfully annotated for their functions (Figure 7). By analyzing the cellular components of the functionally annotated genes, we found that only a few genes played a part in membrane structure formation, such as organelle parts (one gene), membrane-enclosed lumen (one gene), extracellular region (one gene) and membrane (one gene). However, there are many genes that are widely present and have positive effects on molecular function (46 genes), accounting for more than 95% of all genes. We further analyzed the biological processes and found that there were significant differences in the expression of the number of genes, the number of genes annotated for each GO term can be found on the website (https://wego.genomics.cn/view/WEGOID77554007142815, accessed on 21 July 2021).

Expression Analysis of AtruWRKY Genes under Cold Stress
To verify the functions WRKYs play in cold hardiness, the expression patterns of 15 randomly selected AtruWRKY genes in different stages at low temperature (4 • C) (0, 6, 12, 24, 36 and 48 h) were determined by qRT-PCR. The results showed that 15 AtruWRKY genes exhibited significant differences, and most of the genes were highly expressed during 0~12 h under cold treatment ( Figure 8). AtruWRKYs, including AtruWRKY12, AtruWRKY13, AtruWRKY15, AtruWRKY17, AtruWRKY28, AtruWRKY31, AtruWRKY39, AtruWRKY44 and AtruWRKY47, showed the highest expression levels when exposed to low temperature for 12 h. AtruWRKY29, AtruWRKY33, and AtruWRKY51 showed the highest levels at 6 h, however AtruWRKY20 (48 h), AtruWRKY25 (24 h) and AtruWRKY51 (36 h) also showed relatively high expressed level after 12 h of cold treatment. Notably, the AtruWRKY33 gene was only distinct expressed at 6 h, while AtruWRKY15, AtruWRKY17 and AtruWRKY33 were almost non-expressed at 12~48 h.

Discussion
A. truncatum is a well-known and valuable tree species for its graceful maple, elite hardwood and rich medicinal ingredients [44,45]. However, only a few studies have been conducted on its growth development and stress response at molecular level because of incomplete RNA-seq and genome sequence data. Previous studies have found that TFs play a crucial role in plant growth and response to abiotic stress through self-regulation and regulation of downstream target gene expression [46][47][48]. WRKY transcription factors have been reported to be one of the largest gene families and play a pivotal role in flowering, growth development, and abiotic and biotic stress responses in plants [16]. So far, some WRKY family members have been identified and analyzed in plant species such as A. thalliana [49], G. max L. [50], T. aestivum [17] and O. sativa [51]. However, none of the reports are published for the identification and functional role of the WRKY gene family in A. truncatum. This study is the first publicly published analysis and identification of WRKY transcription factors via genomic data, which provided a better understanding of the function of WRKY genes family under cold stress in A. truncatum.
The number of TFs in a gene family is related not only to the genetic background of the species but also to the influence of the long-term evolutionary succession of plants. In present investigation, 54 genes were ultimately identified as encoded by the A. truncatum WRKY family.  [52][53][54][55][56][57], suggesting high conservation of WRKY gene family in A. truncatum, which may be related to gene duplication during species formation and evolution. Furthermore, the subcellular localization results in this study showed that all but AtruWRKY1, AtruWRKY4 and AtruWRKY24 were detected in the nucleus, suggesting that most WRKY gene functions may are closely related to the expression regulation of target genes, similar to Xanthoceras sorbifolium [58] and S. album [52]. The 54 WRKY transcription factors identified, all of which were unevenly distributed on 13 chromosomes in A. truncatum, may play important roles in the evolution of the WRKY gene family. Interestingly, the AtruWRKY14 and AtruWRKY17 have a tandem duplication relationship between them, which were also close phylogenetically. At the same time, transcription factor genes with similar functions were clustered together in evolutionary trees. In particular, group III had significantly more gene members than the other two, accounting for 54% of all AtruWRKYs. This was consistent with the results of P. miliaceum L. [55] and Chinese jujube (Ziziphus jujuba Mill.) [8], indicating the WRKY gene family diversification and conservation among the land plants. In addition, some studies have clarified that the variation of group III WRKY genes may be key to the variation of WRKY transcription factors [59].
The number and species of conserved motifs of each WRKY family member were different to some extent, but the conserved motifs and species of the protein members in the same subgroup were roughly the same, indicating that the members of the WRKY family had similar structure and biological function. All of WRKY genes family members in this paper contained motif 2, indicating that motif 2 with high frequency may be closely related to the molecular function and structural properties involved in WRKY gene. In-depth analysis of the structural characteristics of introns and exons is a key step in the process of gene family evolution [60,61]. A varied number of introns was possessed by AtruWRKY genes, group I had more introns in this study, implying that the molecular structure in the group I of WRKY genes may be quite conserved in the process of evolution, which conductive to the protein diversity caused evolution [62,63]. It seems that the crucial function of the WRKY gene family in A. truncatum is very much related to group I, and similar results were found in eggplant (Solanum torvum L.) [60]. These results confirm the characteristics of the AtruWRKY gene family and facilitate further study on the function of AtruWRKY genes.
RNA-seq is usually used to study gene function and structure at the overall level and reveal the molecular mechanisms of specific biological processes and disease occurrence. This approach has been widely used in basic research, clinical diagnosis, drug development and other fields [64][65][66][67]. As shown in Figure 6, we used transcriptome data from different tissues/organs (root, flower, leaf, seed and stem) of A. truncatum to explore the expression of the WRKY gene family, the expression pattern of each AtruWRKY gene was altered in these tissues/organs. We found that among all 54 AtruWRKY genes, 52 genes (except AtruWRKY49 and AtruWRKY53) were expressed specifically in plant tissues. Among them, most AtruWRKY genes were highly expressed in root (56%), whereas a few AtruWRKY genes were expressed in seed (6%) and stem (8%). This is consistent with studies made in other plants, such as S. indicum L. [54], cabbage (Brassica rapa ssp. pekinensis) [68], grape (Vitis vinifera) [69] and cucumber (Cucumis sativus) [70]. The results showed that the WRKY genes are expressed tissue specifically, and which may function reflect in responses that first affect plants below ground. AtruWRKY2, AtruWRKY8, AtruWRKY9, AtruWRKY22 and AtruWRKY24 showed the highest expression levels in the roots, and these genes may play a key role in the root formation and development of A. truncatum. For instance, previous studies have suggested that TaWRKY51 genes took part in T. aestivum L. lateral roots formation by modulating ethylene biosynthesis [71]. Overexpression AtWRKY75 and OsWRKY31 gave rise to reduced significantly the number of relevant lateral roots in contrast [72,73]. AtruWRKY11 is highly expressed only in flowers, indicating that some AtruWRKY might only be expressed in response to particular biotic and abiotic. These results suggest that WRKY transcription factors exhibit varied expression profiling in various organs or tissues to regulate various biological and physiological metabolism processes in A. truncatum [74,75].
Cold stress is a crucial factor affecting the growth and development of plants. Previous studies on the mechanism by which WRKY regulates cold stress have mainly focused on model plants, for instance, the OsMADS57 and OsTB1 conversely affect O. sativa L. chilling tolerance via targeting OsWRKY94 [76]. In A. thaliana, the AtWRKY34 negatively mediated cold sensitivity of mature pollen, speculated that it might be involved in the CBF signal cascade in mature pollen [77]. In this study, 15 differentially expressed AtruWRKY genes were verified in A. truncatum from the 54 genes ( Figure 8). The results showed that 13 AtruWRKY genes were highly expressed at 0~12 h after low-temperature treatment, which implied that these genes may be crucial in the early stage of cold stress treatment. In contrast, the expression levels of AtruWRKY25 and AtruWRKY51 were slightly increased after 12 h, suggesting that these genes participated in the late reaction to cold treatment. This difference in expression before and after indicates that WRKY transcription factors were time-efficient in response to cold stress, which was also verified in Brassica napus [78] and P. mumu [19] in the past research, suggesting that different WRKY transcription factors may play roles in different periods. In addition, the expression of a few AtruWRKY genes decreased or faded away under cold treatment, such as AtruWRKY17, AtruWRKY31, AtruWRKY33, AtruWRKY39 and AtruWRKY47. We conjecture that these genes may play a role in other biotic and abiotic stresses in A. truncatum.

Conclusions
This study is the first genome-level description of the WRKY gene family of A. truncatum. We identified 54 WRKY genes in A. truncatum, and all of them were located on 13 chromosomes. In addition, we identified ten conserved domains of AtruWRKY proteins, and these WRKY genes were classified into 3 groups (9 subgroups) based on phylogenetic relationships. The collinearity of A. truncatum and A. yangbiense was better than that of A. thaliana, and the functional annotation results showed that the majority of WRKY genes were involved in the regulation of molecular functions and biological processes, such as transcription regulator activity, biological regulation, regulation of biological process, metabolic process, and cellular process, etc. Furthermore, the expression patterns in five different tissues suggested that these WRKY genes might play a crucial role in flowers, seeds, leaves, roots, and stems. Finally, more AtruWRKY genes were significantly highly expressed under 0, 6 and 12 h of cold stress, which provides a meaningful direction for future research under cold stress. The analysis of WRKY genes identifies their molecular mechanisms and potential functions involved in plant biotic and abiotic stress responses in A. truncatum and lays a foundation for the study of WRKY TFs in A. truncatum and other plants.