AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China
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Genes 2020, 11(4), 453; https://doi.org/10.3390/genes11040453
Received: 19 March 2020
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Revised: 10 April 2020
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Accepted: 13 April 2020
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Published: 22 April 2020
(This article belongs to the Special Issue Pig Genomics and Genetics)
The meat quality of animal products is closely related to the intramuscular fat content. Aquaglyceroporin (AQP) defines a class of water/glycerol channels that primarily facilitate the passive transport of glycerol and water across biological membranes. In this study, the AQP3 protein of the AQP family was mainly studied in the adipogenic function of intramuscular adipocytes in pigs. Here, we found that AQP3 was increased at both mRNA and protein levels upon adipogenic stimuli in porcine intramuscular adipocytes in vitro. Western blot results showed knockdown of AQP3 by siRNA significantly suppressed the expression of adipogenic genes (PPARγ, aP2, etc.), repressed Akt phosphorylation, as well as reducing lipid accumulation. Furthermore, deletion of AQP3 by siRNA significantly downregulated expression of cell cycle genes (cyclin D, E), and decreased the number of EdU-positive cells as well as cell viability. Collectively, our data indicate that AQP3 is of great importance in both adipogenic differentiation and proliferation in intramuscular adipocytes, providing a potential target for modulating fat infiltration in skeletal muscles.
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Keywords:
AQP3; pig; intramuscular fat; adipogenesis; proliferation
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MDPI and ACS Style
Wang, X.; Yang, J.; Yao, Y.; Shi, X.; Yang, G.; Li, X. AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes. Genes 2020, 11, 453. https://doi.org/10.3390/genes11040453
AMA Style
Wang X, Yang J, Yao Y, Shi X, Yang G, Li X. AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes. Genes. 2020; 11(4):453. https://doi.org/10.3390/genes11040453
Chicago/Turabian StyleWang, Xiaoyu, Jing Yang, Ying Yao, Xin’E Shi, Gongshe Yang, and Xiao Li. 2020. "AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes" Genes 11, no. 4: 453. https://doi.org/10.3390/genes11040453
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