Next Article in Journal
Cross-Talk between Fibroblast Growth Factor Receptors and Other Cell Surface Proteins
Previous Article in Journal
Involvement of TREK-1 Channel in Cell Viability of H9c2 Rat Cardiomyoblasts Affected by Bupivacaine and Lipid Emulsion
Article Menu

Export Article

Open AccessArticle

Zebrafish Carrying pycr1 Gene Deficiency Display Aging and Multiple Behavioral Abnormalities

1
Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
2
Department of Chemistry, Chung Yuan Christian University, Chung-Li 32023, Taiwan
3
Department of Biological Science & Technology, College of Medicine, I-Shou University, Kaohsiung 84001, Taiwan
4
Department of Chemistry, Chinese Culture University, Taipei 11114, Taiwan
5
Guangxi Scientific Experimental Center of Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning 530200, China
6
Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning 530200, China
7
Department of Pediatrics, Mackay Memorial Hospital, Taipei 252, Taiwan
8
Department of Medical Research, Mackay Memorial Hospital, Taipei 252, Taiwan
9
Department of Medicine, Mackay Medical College, New Taipei 252, Taiwan
10
Center for Biomedical Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
11
Center for Nanotechnology, Chung Yuan Christian University, Chung-Li 32023, Taiwan
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(5), 453; https://doi.org/10.3390/cells8050453
Received: 28 February 2019 / Revised: 28 April 2019 / Accepted: 9 May 2019 / Published: 14 May 2019
  |  
PDF [6916 KB, uploaded 14 May 2019]
  |  

Abstract

Aging is a natural process that internal gene control and external stimuli mediate. Clinical data pointed out that homozygotic or heterozygotic mutation in the pyrroline-5-carboxylate reductase 1 (PYCR1) gene in humans caused cutis laxa (ARCL) disease, with progeroid appearance, lax and wrinkled skin, joint laxity, osteopenia, and mental retardation phenotypes. In this study, we aimed to generate pycr1 knockout (KO) zebrafish and carried out biochemical characterizations and behavior analyses. Marked apoptosis and senescence were detected in pycr1 KO zebrafish, which started from embryos/larvae stage. Biochemical assays showed that adult pycr1 KO fish have significantly reduced proline and extracellular matrix contents, lowered energy, and diminished superoxide dismutase (SOD) and telomerase activity when compared to the wild type fish, which suggested the pycr1 KO fish may have dysfunction in mitochondria. The pycr1 KO fish were viable; however, displayed progeria-like phenotype from the 4 months old and reach 50% mortality around six months old. In adult stage, we found that pycr1 KO fish showed reduced locomotion activity, aggression, predator avoidance, social interaction interest, as well as dysregulated color preference and circadian rhythm. In summary, we have identified multiple behavioral alterations in a novel fish model for aging with pycr1 gene loss-of-function by behavioral tests. This animal model may not only provide a unique vertebrate model to screen potential anti-aging drugs in the future, but also be an excellent in vivo model towards a better understanding of the corresponding behavioral alterations that accompany aging. View Full-Text
Keywords: zebrafish; TALEN; PYCR1; aging; disease model; behavioral alteration zebrafish; TALEN; PYCR1; aging; disease model; behavioral alteration
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Liang, S.-T.; Audira, G.; Juniardi, S.; Chen, J.-R.; Lai, Y.-H.; Du, Z.-C.; Lin, D.-S.; Hsiao, C.-D. Zebrafish Carrying pycr1 Gene Deficiency Display Aging and Multiple Behavioral Abnormalities. Cells 2019, 8, 453.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top