Correction: Dastghaib et al. Simvastatin Induces Unfolded Protein Response and Enhances Temozolomide-Induced Cell Death in Glioblastoma Cells. Cells 2020, 9, 2339

In the original publication [...].

In the original publication [1], there was a mistake in Figures 4A, 6A, 7A and 9A as published.The protein loading controls (GAPDH) were the same.The corrected Figures 4A, 6A, 7A and 9A appear below.The authors state that the scientific conclusions are unaffected.This correction was approved by the Academic Editor.The original publication has also been updated.(A) U87 and U251 were pretreated with PERKi (5 µM, 30 min) and then co-treated with Simva-TMZ for 72 h.The protein levels of eIF2α and p-eIF2α were determined using immunoblotting; GAPDH was used as a loading control.(B,C) Densitometric analysis of the immunoblots showed that Simva-TMZ by itself significantly reduced the p-eIF2α/eIF2α ratio, which was not further decreased by the PERKi in either cell line.Of note, control levels of p-eIF2α were significantly decreased by the PERKi as well.The data are expressed as the means ± SD of three independent experiments ** p < 0.01; **** p < 0.0001).Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s).MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Figure 6 .
Figure 6.PERK inhibition does not change the p-eIF2α/eIF2α ratio in Simva-TMZ-treated cells.(A)U87 and U251 were pretreated with PERKi (5 µM, 30 min) and then co-treated with Simva-TMZ for 72 h.The protein levels of eIF2α and p-eIF2α were determined using immunoblotting; GAPDH was used as a loading control.(B,C) Densitometric analysis of the immunoblots showed that Simva-TMZ by itself significantly reduced the p-eIF2α/eIF2α ratio, which was not further decreased by the PERKi in either cell line.Of note, control levels of p-eIF2α were significantly decreased by the PERKi as well.The data are expressed as the means ± SD of three independent experiments ** p < 0.01; **** p < 0.0001).

Figure 6 .
Figure 6.PERK inhibition does not change the p-eIF2α/eIF2α ratio in Simva-TMZ-treated cells.(A)U87 and U251 were pretreated with PERKi (5 µM, 30 min) and then co-treated with Simva-TMZ for 72 h.The protein levels of eIF2α and p-eIF2α were determined using immunoblotting; GAPDH was used as a loading control.(B,C) Densitometric analysis of the immunoblots showed that Simva-TMZ by itself significantly reduced the p-eIF2α/eIF2α ratio, which was not further decreased by the PERKi in either cell line.Of note, control levels of p-eIF2α were significantly decreased by the PERKi as well.The data are expressed as the means ± SD of three independent experiments ** p < 0.01; **** p < 0.0001).

Figure 7 .
Figure 7. PERK inhibition differentially affects autophagy flux in U87 and U251 cells treated with Simva-TMZ.(A) U87 and U251 cells were pretreated GSK PERK inhibitor (5 µM, 30 min) and then co-treated with Simva-TMZ as described for 72 h.The protein levels of p62, LC3β-II, and LCβ-I were determined by immunoblotting.Simva-TMZ induced an inhibition of autophagy flux (accumulation of p62 and LC3β-II) in GBM cells.The PERKi decreased p62 degradation (autophagosome degradation) in both U87 and U251 cells, while it increased the LC3β-II/LC3β-I ratio in U251 cells and decreased it in U87 cells.GAPDH was used as a loading control.(B-E) Densitometric analysis of the Western blot bands to quantify p62 and LC3β-II/LC3β-I protein amount.Data are expressed as the mean ± SD of three independent experiments (** p < 0.01; **** p < 0.0001).

Figure 7 .
Figure 7. PERK inhibition differentially affects autophagy flux in U87 and U251 cells treated with Simva-TMZ.(A) U87 and U251 cells were pretreated GSK PERK inhibitor (5 µM, 30 min) and then co-treated with Simva-TMZ as described for 72 h.The protein levels of p62, LC3β-II, and LCβ-I were determined by immunoblotting.Simva-TMZ induced an inhibition of autophagy flux (accumulation of p62 and LC3β-II) in GBM cells.The PERKi decreased p62 degradation (autophagosome degradation) in both U87 and U251 cells, while it increased the LC3β-II/LC3β-I ratio in U251 cells and decreased it in U87 cells.GAPDH was used as a loading control.(B-E) Densitometric analysis of the Western blot bands to quantify p62 and LC3β-II/LC3β-I protein amount.Data are expressed as the mean ± SD of three independent experiments (** p < 0.01; **** p < 0.0001).