Runx3 Restoration Regresses K-Ras-Activated Mouse Lung Cancers and Inhibits Recurrence

Oncogenic K-RAS mutations occur in approximately 25% of human lung cancers and are most frequently found in codon 12 (G12C, G12V, and G12D). Mutated K-RAS inhibitors have shown beneficial results in many patients; however, the inhibitors specifically target K-RASG12C and acquired resistance is a common occurrence. Therefore, new treatments targeting all kinds of oncogenic K-RAS mutations with a durable response are needed. RUNX3 acts as a pioneer factor of the restriction (R)-point, which is critical for the life and death of cells. RUNX3 is inactivated in most K-RAS-activated mouse and human lung cancers. Deletion of mouse lung Runx3 induces adenomas (ADs) and facilitates the development of K-Ras-activated adenocarcinomas (ADCs). In this study, conditional restoration of Runx3 in an established K-Ras-activated mouse lung cancer model regressed both ADs and ADCs and suppressed cancer recurrence, markedly increasing mouse survival. Runx3 restoration suppressed K-Ras-activated lung cancer mainly through Arf-p53 pathway-mediated apoptosis and partly through p53-independent inhibition of proliferation. This study provides in vivo evidence supporting RUNX3 as a therapeutic tool for the treatment of K-RAS-activated lung cancers with a durable response.


Introduction
Lung adenocarcinoma (ADC) is the most frequent subtype of lung cancer.Most lung ADCs develop through stepwise progression from adenoma (AD) to ADCs [1,2].Approximately 25% of human lung ADC cases harbor activating mutations in the K-RAS gene [3].The most frequent mutations occur in codon 12, and the most common subtypes are G12C, G12V, and G12D.Recently, new drugs targeting a specific type of K-RAS mutation (K-RAS G12C ) were conditionally approved by the USA Food and Drug Administration [4].The inhibitors effectively regress K-RAS G12C -mutated lung cancers.However, the cancers commonly recur within 1 year.The acquired drug resistance is mainly due to secondary oncogenic mutations occurring at oncogenic K-RAS itself or at both upstream (EGFR, HER2, FGFR) and downstream (MAPK/MEK pathway) sites [5][6][7].This rapid recurrence after inhibition of oncogenic K-RAS was observed previously in a mouse lung cancer model in which tumors initially responded to knockdown of oncogenic K-Ras but recurred after 2 weeks with secondary oncogene activation [8].These results indicate that existing strategies for inhibiting oncogenic K-RAS have a limited ability to achieve a durable response in the context of cancer treatment.
To establish new treatments with a durable response, it is important to determine whether cells have evolved effective defense mechanisms against oncogene activation.Normal cells have a defense mechanism against oncogenic K-Ras involving the Arf-p53 pathway [9][10][11].Simultaneous activation of K-Ras and inactivation of p53 in the mouse lung accelerates malignant progression to ADC [12].Considering the pro-apoptotic function of p53, restoration of p53 is considered an attractive therapeutic intervention.However, p53 restoration in K-Ras-activated mouse lung cancer suppresses ADCs but does not affect ADs, which are likely to develop into ADCs [13][14][15].Consistent with this, heterozygous oncogenic K-Ras mutations induce lung AD/ADC in the absence of p53 mutation [16], and loss of p53 does not have a significant impact on early K-Ras-induced lung tumorigenesis [17].Whether cells have evolved effective defense mechanisms against heterozygous oncogenic K-Ras mutations remains unclear.
A tumor is defined as an abnormal mass of tissue that forms when cells divide at a higher rate than normal or do not die when they should.The cellular decision regarding whether to undergo division or death is made at the restriction (R)-point, which is disrupted in nearly all tumors [18].RUNX3 functions as a pioneer factor of the R-point and leads to a death decision in response to aberrant persistence of RAS signals [19][20][21][22][23]. When a death decision is made at the R-point, the cellular defense against tumorigenesis involves activating the ARF-p53 pathway [20].RUNX3 is deactivated by epigenetic alterations in most cases of K-RAS-activated mouse and human lung ADCs [24,25].Runx3 inactivation not only abrogates R-point-associated Arf-p53 pathway activity but also promotes the formation of lung ADs and accelerates the development of oncogenic K-Ras-dependent ADCs [25].
Conceivably, lung AD cells that develop as a result of Runx3 inactivation are unable to defend against oncogenic K-Ras, resulting in the transition from AD to ADC upon oncogenic K-Ras mutation.However, it remains unclear whether Runx3 can effectively regress alreadyestablished K-Ras-activated lung AD and ADC in vivo.To address this question, we developed a mouse model in which Runx3 is conditionally restored by inducible Flippase.This mouse model was used to demonstrate that Runx3 restoration effectively regresses established lung cancers and inhibits recurrence.The results indicate that cells have evolved an effective defense mechanism against heterozygous oncogenic K-Ras mutation.The mechanism is abrogated by Runx3 inactivation and can be re-established with Runx3 restoration.These results support the potential of RUNX3 as a therapeutic tool for the treatment of K-RAS-activated lung cancers with a durable response.

Mice
Runx3 flox (Jax 008773), p53 flox (Jax 008462), K-Ras LSL-G12D (Jax 008179), K-Ras LA1 (Johnson et al., 2001), Rosa26R-Tomato (Jax 007914), and Flp ERT2 (R26 FlpoER , Jax 019016) mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA).Runx3 Frt-Stop-Frt/+ (Runx3 FSF/+ ) mice originated from Macrogen (Seoul, Republic of Korea).All mice analyzed had mixed genetic backgrounds and were age matched (6-8 weeks old) unless mentioned specifically.Sample size was determined based on our experience and previous experiments.No data were excluded from analysis.Animal experiments described were repeated with at least three independent replicates with significant results in the same direction as those represented in the figures.All animal studies were randomized in 'control' or 'treated' groups.However, all animals housed within the same cage were generally placed within the same treatment group.For analysis of tumor samples, identities were blinded from histopathological assessment.All animals were housed in SPF (Specific Pathogen Free) facilities.The animal studies were approved by the Institutional Animal Care Committee of Chungbuk National University.

Adenovirus and Tamoxifen Delivery in Mice
Adenovirus carrying Cre recombinase (Ad-Cre) was purchased from Vector Biolabs (Philadelphia, PA, USA).Each mouse was treated with a titer of 2.5 × 10 7 Ad-Cre viral genome copies diluted in 50 µL warm sterile MEM.After the treatment, mice were placed on a warm pad until they woke up.Tamoxifen-containing food was administered every day at 400 mg/kg food (#TD.130860,Teklad diet; ENVIGO, Somerset, NJ, USA).Animal studies were approved by the Institutional Animal Care Committee of Chungbuk National University.Animals were maintained under specific pathogen-free conditions and monitored daily.

KPR Primary Tumor Cell Line Extract
We prepared 6-to 8-week-old K-Ras LSL-G12D/+ , p53 flox/flox , Runx3 flox/FSF , Tomato*, and Flp ERT2 mice (KPR L/F mice) and nasally infected them with Ad-Cre to induce oncogenic K-Ras-dependent lung cancer.Four weeks after the virus infection, we sacrificed the mice and extracted their lungs to obtain the KPR primary tumor cell line.The tumor burden from the lung was sliced into tiny pieces and treated with trypsin-EDTA so that the KPR primary tumor cells were isolated.The cells were stabilized in 20% FBS-containing DMEM for days and cultured in 10% FBS-containing DMEM.

Hematoxylin and Eosin (HE) Staining
H&E staining experiments were performed following standard protocols.Briefly, slides were rehydrated with ethanol, xylene, and water to remove the paraffin.The nuclei were stained with hematoxylin (DAKO, CA, USA, #S3309) for 3 min and the cytoplasm was stained with eosin (Sigma, HT110280) for 30 seconds.Slides were mounted with Permount (Fisher Scientific, SP15-500) after the dehydration and clearing steps.

Histology and Immunohistochemistry
For histological analyses, lungs were inflated with 4% paraformaldehyde or formalin (3.7% formaldehyde) and fixed for 36 h.Fixed paraffin sections were rehydrated, subjected to antigen retrieval, blocked in TBS (0.1% Triton X-100 containing 1% BSA) or DAKO protein-free blocking solution, and sequentially incubated with specific primary antibodies, biotinylated secondary antibodies (DAKO), and the Alexa Fluor system (Invitrogen, Waltham, MA, USA).Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using the in situ Cell Death Detection kit (Roche).Images were produced using a conventional microscope mounted with a DP71 digital camera (Olympus), an LSM 710 T-PMT confocal microscope (Carl Zeiss), and an AXIO Zoom.V16 and ApoTome.2 (Carl Zeiss).Images were processed with equivalent parameters using the ZEN Light Edition software (Carl Zeiss) https://www.zeiss.com.cn/microscopy/products/microscope-software/zen-lite/zen-lite-download.htmlaccessed on 6 October 2023.

DNA Exon-Seq Analysis
For the generation of standard exome capture libraries, the Agilent SureSelect Target Enrichment protocol for the Illumina paired-end sequencing library (ver.B.3, June 2015) was used with 1 µg input gDNA.In all cases, the SureSelect Human All Exon V6 or SureSelect Mouse All Exon probe set was used.DNA quantification and DNA quality were analyzed using PicoGreen and agarose gel electrophoresis.One microgram of genomic DNA from each cell line was diluted in EB buffer and sheared to a target peak size of 150-200 bp using the Covaris LE220 focused ultrasonicator (Covaris, Woburn, MA, USA) according to the manufacturer's recommendations.The 8 microTUBE Strip was loaded into the tube holder of the ultrasonicator, and the DNA was sheared using the following settings: mode, frequency sweeping; duty cycle, 10%; intensity, 5; cycles per burst, 200; duration, 60 sec × 6 cycles; and temperature, 4-7 • C. The fragmented DNA was repaired, an 'A' was ligated to the 3 end, and Agilent adapters were then ligated to the fragments.Once ligation was assessed, the adapter-ligated product was PCR amplified.The final purified product was quantified using the TapeStation DNA screentape D1000 (Agilent, Santa Clara, CA, USA).For exome capture, 250 ng of the DNA library was mixed with hybridization buffers, blocking mixes, RNase block, and 5 µL of SureSelect all-exon capture library, according to the standard Agilent SureSelect Target Enrichment protocol.Hybridization to the capture baits was performed at 65 • C using the heated thermal cycler lid option at 105 • C for 24 h on a PCR machine.The captured DNA was then washed and amplified.The final purified product was quantified using qPCR according to the qPCR Quantification Protocol Guide (KAPA Library Quantification kits for Illumina Sequencing platforms), analyzed using the TapeStation DNA screentape D1000 (Agilent), and sequenced using the HiSeq™ 2500 platform (Illumina, San Diego, CA, USA).

DNA Transfection, IP, and IB
Transient transfections in all cell lines were performed using Lipofectamine Plus reagent and Lipofectamine (Invitrogen).Cell lysates were incubated with the appropriate mono-or polyclonal antibodies (2 µg antibody/500 µg lysate sample) for 3 h at 4 • C, and then with protein G-Sepharose beads (Amersham Pharmacia Biotech, Piscataway, NJ, USA) for 1 h at 4 • C. For detection of endogenous proteins, lysates were incubated with the appropriate mono-or polyclonal antibodies (dilution range 1:1000-1:3000) for 6-12 h at 4 • C, and then with protein G-Sepharose beads (Amersham Pharmacia Biotech) for 3 h at 4 • C. Immunoprecipitates were resolved using SDS-polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA).The membrane was immunoblotted with the appropriate antibodies after blocking and visualized on an Amersham™ Imager 600 (GE Healthcare, Chicago, IL, USA) after treatment with ECL solution (Amersham Pharmacia Biotech).

RNA-Seq Analysis
Total RNA was isolated using Trizol reagent (Invitrogen).RNA quality was assessed using an Agilent 2100 bioanalyzer (Agilent Technologies, Amstelveen, The Netherlands) and RNA quantification was performed using an ND-2000 Spectrophotometer (Thermo Inc., DE, USA).Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (NEW ENGLAND BioLabs, Inc., UK). Isolation of mRNA was performed using the Poly(A) RNA Selection Kit (LEXOGEN, Inc., Austria).The isolated mRNAs were used for cDNA synthesis and shearing in accordance with the manufacturer's instructions.Indexing was performed using the Illumina indexes 1-12.The enrichment step was carried out using PCR.Subsequently, libraries were checked using the TapeStation HS D1000 Screen Tape (Agilent Technologies, Amstelveen, The Netherlands) to evaluate the mean fragment size.Quantification was performed using the library quantification kit and a StepOne Real-Time PCR System (Life Technologies, Inc., Carlsbad, CA, USA).High-throughput sequencing was performed as paired-end 100 sequencing using NovaSeq 6000 (Illumina, Inc., San Diego, CA, USA).

Quantification and Statistical Analysis
Quality control of raw sequencing data was performed using FastQC [26].Adapters and low-quality reads (<Q20) were removed using FASTX_Trimmer [27] and BBMap [28].Then, the trimmed reads were mapped to the reference genome using TopHat [29].The Read Count data were processed based on an FPKM+ Geometric normalization method Cells 2023, 12, 2438 5 of 18 using EdgeR within R [30].FPKM (fragments per kb per million reads) values were estimated using Cufflinks [31].Data mining and graphic visualization were performed using ExDEGA (Ebiogen Inc., Seoul, Republic of Korea).Gene clustering was analyzed using DAVID Bioinformatics Resources 2021 [32].

Results
3.1.Generation of K-Ras LoxP-Stop-LoxP-G12D/+ , Runx3 flox/FSF , Tomato*, and Flp ERT2 (KR L/F ) Mice To determine the roles of Runx3 in K-Ras-activated lung ADCs, we developed a mouse model, Runx3 Frt-Stop-Frt (hereafter Runx3 FSF ), in which Runx3 is deactivated by a Frt-Stop-Frt cassette, but can be conditionally restored via deletion of the cassette through the activation of Flippase recombinase (Flp) (Figures 1A and S1).Runx3 FSF/+ mice were indistinguishable from Runx3 +/− mice, and Runx3 FSF/FSF mice, similar to conventional Runx3 −/− mice [33], died within 24 h after birth.Six weeks after Ad-Cre infection, the mice were fed normal food (KR L/F -TAM(-) group, n = 5) or tamoxifen-containing food (KR L/F -TAM(+) group, n = 5) for two weeks, followed by normal food in all mice.The median survival of the KR L/F -TAM(-) group and the KR L/F -TAM(+) group were 13.2 weeks and 28.2 weeks, respectively (p = 0.000001).(E) Gross images of Tomato fluorescence emitted under UV light from the lungs of KR L/F -TAM(-)-4w mice (control mice, Figure 2B) and microscopic images of the lungs stained with HE (left) or anti-Tomato antibody (right).(F,G) Gross images of Tomato fluorescence emitted under UV light from the lungs of KR L/F -TAM(+)-4w mice and KR L/F -TAM(+)-10w mice (fed tamoxifen-containing food, Figure 2B), and microscopic images of the lungs stained with HE (left) or anti-Tomato antibody (right).

Runx3 Restoration Effectively Eliminates Established K-Ras-activated Lung Cancer Cells
To determine whether Runx3 restoration could regress already-established K-Rasactivated lung ADs and/or ADCs, the KR L/F mice were infected with Ad-Cre through nasal inhalation (2.5 × 10 7 pfu/mouse) [12,25] for K-Ras activation and Runx3 inactivation.Six weeks after Ad-Cre infection, the lungs of the mice exhibited Tomato fluorescence under UV light, indicating tumor development (Figure 1C).Microscopic analysis confirmed that the mice developed many lung ADs/ADCs (Figure 1C).The remaining mice were fed normal food (KR L/F -TAM(-), n = 5) or tamoxifen-containing food (KR L/F -TAM(+), n = 5) to promote Runx3 restoration.KR L/F -TAM(-) mice survived for an average of 13.2 weeks after infection, and all died by 14 weeks after infection (Figure 1D).In contrast, all KR L/F -TAM(+) mice survived for an average of 28.2 weeks after infection, and all died by 30 weeks after infection (Figure 1D).These results demonstrate that Runx3 restoration extends the survival of lung-cancer-induced KR L/F mice by 15 weeks.
In a parallel experiment, KR L/F -TAM(-) and KR L/F -TAM(+) mice were sacrificed at 4 or 10 weeks after tamoxifen treatment (Figure 1D).KR L/F -TAM(-)-4w mouse lungs exhibited high levels of Tomato fluorescence under UV light and developed large lung ADs/ADCs (Figure 1E).However, the lungs of KR L/F -TAM(+)-4w mice exhibited very low levels of Tomato fluorescence under UV light (Figure 1F).The number of Tomato-positive cells did not increase until 10 weeks after Runx3-restoration (Figure 1G).Enlarged microscopic images of the figure are shown in Supplementary Figure S2.Once the Rosa26R-Tomato allele is targeted, Tomato fluorescence is maintained throughout the lifespan of the targeted cells.Therefore, the rare Tomato-positive cells detected in the lungs of KR L/F -TAM(+)-4w and KR L/F -TAM(+)-10w mice suggest that nearly all the K-Ras-activated lung AD cells and ADC cells were eliminated through Runx3 restoration.Genotyping of the lung tumors confirmed K-Ras activation, Runx3 inactivation by Ad-Cre infection, and Runx3 restoration via tamoxifen treatment (Supplementary Figure S3).

Runx3 Restoration Effectively Eliminates Established K-Ras-activated Lung Cancer Cells
To determine whether Runx3 restoration could regress already-established K-Ras-activated lung ADs and/or ADCs, the KR L/F mice were infected with Ad-Cre through nasal inhalation (2.5 × 10 7 pfu/mouse) [12,25] for K-Ras activation and Runx3 inactivation.Six

Runx3 Restoration Eliminates K-Ras-activated Lung Cancers by Inducing Apoptosis
To elucidate the mechanism by which Runx3 restoration eliminated K-Ras-activated lung ADs and ADCs, we obtained lungs from Ad-Cre-infected KR L/F mice 1 week after tamoxifen treatment.Tomato and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining of the mouse lungs showed that the Tomato-positive lung cancer cells of KR L/F -TAM(-)-1w mice were TUNEL-negative.However, most of the Tomatopositive lung cancer cells of KR L/F -TAM(+)-1w mice were TUNEL-positive (Figure 2A), indicating that the Runx3-restored cells underwent apoptosis.Enlarged microscopic images of the figure are shown in Supplementary Figure S4.We previously reported that Arf is a major target of Runx3 [20]; consistently, Runx3 restoration induced Arf and p53 expression in the lung cancers (Figure 2A).Enlarged microscopic images of the figure are shown in Supplementary Figure S5.Consistent with this, the lungs of KR L/F -TAM(+)-4w mice contained a few lesions that were undergoing regression, and the cells in these lesions were TUNEL-positive (Figure 2B,C).These results demonstrate that Runx3 restoration activates the Arf-p53 pathway and eliminates K-Ras-activated lung cancer cells by inducing apoptosis.

K-Ras-Activated Lung Cancer Began to Recur at 14 Weeks after Runx3 Restoration
All of the Runx3-restored mice began to die at 20 weeks after tamoxifen treatment (26 weeks after Ad-Cre infection) (Figure 1D).In a parallel experiment, KR L/F -TAM(+) mice were sacrificed at 14 weeks after tamoxifen treatment (Figure 1D).Analysis of the KR L/F -TAM(+)-14w mouse lungs showed that small ADCs had developed in all four mice (Figure 3A).The ADCs were Tomato-positive, suggesting that the cancers recurred from remnant K-Ras-activated cells (Figure 3A).Whole-exon sequencing indicated that K-Ras was mutated (K-Ras G12D ) in KR L/F -TAM(-)-0w mouse lung ADCs and recurrent lung ADCs, as targeted in the K-Ras LSL-G12D allele.There were no additional mutations in K-Ras, and none of the other known major oncogenes (Egfr, B-Raf, Alk, Mek, Stk11, Smarca4, and Pi3ka) or tumor suppressors (Rb1, Apc, and p53) involved in lung cancer were mutated in any of the ADCs (Figure 3B and Supplementary Data S1).This suggests that secondary oncogene activation was not involved in the cancer recurrence.
Genotyping of the recurrent lung ADCs confirmed that the Stop cassette was removed from the Runx3 FSF allele by tamoxifen-activated Flp ERT2 (Figure 3C).However, immunostaining showed that Runx3 expression was considerably lower in the majority of the recurrent ADCs than in the adjacent normal region (Figure 3D).Methylation-specific PCR (MS-PCR) showed that the CpG island of Runx3 was hyper-methylated in six of eight recurred ADCs (Figure 3E).These results suggest that the restored Runx3 allele was spontaneously inactivated, mainly through DNA hyper-methylation in the recurred lung ADCs.Therefore, it is likely that the quiescent K-Ras-activated cells that remained after Runx3 restoration re-established lung tumors due to spontaneous silencing of the restored Runx3 allele.The nucleotide sequence of the Runx3 CpG island subjected to MS-PCR and the PCR primers used are shown in Supplementary Figure S6.

Runx3
Inactivation Is Essential for the Maintenance of K-Ras-Activated Lung Cancer K-Ras LA1/+ mice, a mouse strain carrying oncogenic alleles of K-Ras that can be activated by a spontaneous recombination event, develop a range of tumor types, predominantly lung cancer [16].To understand the role of Runx3 in lung tumorigenesis activated by K-Ras alone, we crossed K-Ras LA1/+ mice with Runx3 FSF/+ mice and Flp ERT2 mice, yielding K-Ras LA1/+ ; Runx3 +/+ (K LA1 R +/+ ) and K-Ras LA1/+ ; Runx3 FSF/+ ; and Flp ERT2 (K LA1 R FSF/+ ) mice (Figure 4A).In the K LA1 R FSF/+ mice, one allele of Runx3 is wild type and the other allele (Runx3 FSF ) is deactivated by the Frt-Stop-Frt cassette.The Runx3 FSF allele can be restored by tamoxifen, which activates Flp ERT2 .
We measured the lifespan of K LA1 R +/+ mice and K LA1 R FSF/+ mice in the absence of tamoxifen.The K LA1 R +/+ mice (n = 15) developed lung cancer and began to die at 26 weeks after birth, and all the mice died within 72 weeks after birth (average lifespan, 51.7 weeks) (Figure 4B,C).The K LA1 R FSF/+ mice (n = 18) began to die at 10 weeks after birth, and all the mice died within 70 weeks after birth (the average lifespan was 39.5 weeks, which is 12.2 weeks shorter than that of K LA1 R +/+ mice) (Figure 4B,C).These results demonstrate that inactivation of one allele of Runx3 significantly shortened the survival of K-Ras LA1/+ mice (p = 0.04).
In a parallel experiment, K LA1 R FSF/+ mice were fed tamoxifen-containing food for Runx3 restoration for 2 weeks starting at 10 weeks after birth (K LA1 R FSF/+ -TAM(+), n = 15).Runx3 restoration significantly extended the survival of the K LA1 R FSF/+ mice: K LA1 R FSF/+ -TAM(+) mice survived for an average of 52.9 weeks after birth, which is 13.4 weeks longer than the K LA1 R FSF/+ -TAM(-) mice (p = 0.02) (Figure 4B,C).The survival curve and the average lifespan of K LA1 R FSF/+ -TAM(+) mice were similar to that of K LA1 R +/+ mice (Figure 4B,C).MS-PCR analysis of the lung ADCs of the K LA1 R FSF/+ -TAM(-) mice and K LA1 R FSF/+ -TAM(+) mice revealed that Runx3 was silenced by CpG island hyper-methylation in all the analyzed lung ADCs (Figure 4D,E).Immunostaining analysis confirmed that the level of Runx3 was considerably lower in ADC cells than in adjacent normal cells (Figure 4F).These results are consistent with the previous observation that Runx3 is silenced by CpG island hyper-methylation in nearly all the lung ADCs activated by K-Ras alone [25].Schematic representation of the experimental strategy used for examining the role of Runx3 in the maintenance of mouse lung cancer induced through K-Ras-activation alone.The K LA1 R FSF/+ mice bear the K-Ras LA1/+ , Runx3 FSF/+ , and Flp ERT2 alleles.In the K LA1 R FSF/+ mice, K-Ras was activated through spontaneous recombination.Treatment with tamoxifen (TAM) restored one allele of Runx3 by activating Flp ERT2 , which deleted the Frt-Stop-Frt cassette from the Runx3 FSF allele.(B) Survival curves of K LA1 R +/+ mice and K LA1 R FSF/+ mice infected with Ad-Cre.Ten weeks after birth, the K LA1 R FSF/+ mice were fed normal food (K LA1 R FSF/+ -TAM(-), n = 15) or tamoxifen-containing food (K LA1 R FSF/+ -TAM(+), n = 15) for two weeks, followed by normal food in all the mice.(C) Statistical analysis of the lifespan of K LA1 R +/+ mice and K LA1 R FSF/+ mice treated with or without tamoxifen.(D,E) DNA methylation of the Runx3 CpG island detected through MS-PCR in lung ADCs developed in 50-week-old K LA1 R FSF/+ -TAM(-) mice and K LA1 R FSF/+ -TAM(+) mice.M, methylated Runx3 CpG island; U, unmethylated Runx3 CpG island.(F) Runx3 expression detected with anti-Runx3 antibody (1E10) in lung ADCs (A) Schematic representation of the experimental strategy used for examining the role of Runx3 in the maintenance of mouse lung cancer induced through K-Ras-activation alone.The K LA1 R FSF/+ mice bear the K-Ras LA1/+ , Runx3 FSF/+ , and Flp ERT2 alleles.In the K LA1 R FSF/+ mice, K-Ras was activated through spontaneous recombination.Treatment with tamoxifen (TAM) restored one allele of Runx3 by activating Flp ERT2 , which deleted the Frt-Stop-Frt cassette from the Runx3 FSF allele.(B) Survival curves of K LA1 R +/+ mice and K LA1 R FSF/+ mice infected with Ad-Cre.Ten weeks after birth, the K LA1 R FSF/+ mice were fed normal food (K LA1 R FSF/+ -TAM(-), n = 15) or tamoxifen-containing food (K LA1 R FSF/+ -TAM(+), n = 15) for two weeks, followed by normal food in all the mice.(C) Statistical analysis of the lifespan of K LA1 R +/+ mice and K LA1 R FSF/+ mice treated with or without tamoxifen.(D,E) DNA methylation of the Runx3 CpG island detected through MS-PCR in lung ADCs developed in 50-week-old K LA1 R FSF/+ -TAM(-) mice and K LA1 R FSF/+ -TAM(+) mice.M, methylated Runx3 CpG island; U, unmethylated Runx3 CpG island.(F) Runx3 expression detected with anti-Runx3 antibody (1E10) in lung ADCs developed in 50-week-old K LA1 R FSF/+ -TAM(+) mice.Magnified images of the boxed regions are shown.(G) Spontaneous activation of the K-Ras LA1/+ allele in lung ADCs developed in K LA1 R +/+ , K LA1 R FSF/+ -TAM(-), and K LA1 R FSF/+ -TAM(+) mice was confirmed via immunoblotting with anti-K-Ras G12D antibody.(H) The restoration of the Runx3 FSF allele in ADCs developed in K LA1 R FSF/+ -TAM(+) mice was verified through genomic PCR.Band 2 indicates restoration of Runx3.The sizes of PCR products and applied primers are shown.Schematic depictions of the alleles before or after targeting with Ad-Cre or tamoxifen, along with the predicted sizes of the PCR products, are shown in Supplementary Figure S7.N = normal tissue (tail) before tamoxifen treatment, T = lung ADC.
If Runx3 inactivation is essential for the maintenance of K-Ras-activated lung cancer, the survival of the model mice for activation via K-Ras alone should be directly related to the number of functional Runx3 alleles.K LA1 R +/+ mice have two functional Runx3 alleles.K LA1 R FSF/+ -TAM(-) mice have only one functional Runx3 allele because the other allele is inactivated.K LA1 R FSF/+ -TAM(+) mice have two functional Runx3 alleles because the inactivated Runx3 allele is restored.The survival of the mouse models was indeed directly related to the number of functional Runx3 alleles (Figure 4B,C).These results confirm that Runx3 inactivation is essential for the maintenance of lung cancer activated by K-Ras alone.

The Tumor-Suppressive Activity of Runx3 Is Largely Dependent on p53
Next, we investigated whether activation of the Arf-p53 pathway is essential for the regression of K-Ras-activated lung cancers induced via Runx3 restoration.For this purpose, we crossed p53 flox/flox mice with KR L/F mice and obtained K-Ras LSL-G12D/+ , p53 flox/flox , Runx3 flox/FSF , Tomato*, and Flp ERT2 mice (KPR L/F mice).In KPR L/F mice, Ad-Cre infection activated K-Ras LSL-G12D , deactivated p53 flox and Runx3 flox , and labeled the targeted cells with Tomato fluorescence (Figure 5A).Treatment with tamoxifen restored Runx3 by deleting the Frt-Stop-Frt cassette from the Runx3 FSF allele via activation of Flp ERT2 (Figure 5A).
Two weeks after Ad-Cre infection, KPR L/F mice developed lung cancer (Figure 5B).Microscopy revealed that the cancers that developed in the KPR L/F mice showed nuclear pleomorphism with prominent nucleoli and scattered cancer giant cells, as well as more advanced histopathology than that of cancers developed in KR L/F mice (Figure 5C).The Ad-Cre-infected KPR L/F mice began to die at 8 weeks after Ad-Cre infection, and all the mice died within 11 weeks (median survival, 9.8 weeks) (Figure 5D).The median survival of KR L/F mice was 13.2 weeks (Figures 1D and 5D).These results indicate that the lifespan of KPR L/F mice was approximately 3.4 weeks shorter than that of KR L/F mice (p = 0.0018).
After confirming tumor development in KPR L/F mice (2 weeks after Ad-Cre infection, Figure 5B), the mice were fed normal (KPR L/F -TAM(-)) or tamoxifen-containing food (KPR L/F -TAM(+)) for 2 weeks (Figure 5E).The median survival was 9.8 weeks in KPR L/F -TAM(-) mice and 14.2 weeks in KPR L/F -TAM(+) mice (Figure 5E).These results demonstrate that Runx3 restoration extended the survival of KPR L/F mice by 4.4 weeks (p = 0.0004).Although Runx3 restoration significantly extended the survival of KPR L/F mice (≈4.4 weeks), a comparison of the effect of Runx3 restoration with that on KR L/F mice (>18 weeks) (Figure 1D) indicates that the tumor-suppressive activity of Runx3 is largely dependent on p53.
Genotyping of the KPR L/F -TAM(-) and KPR L/F -TAM(+) lung cancers confirmed targeting of K-Ras LSL-G12D , p53 flox , and Runx3 flox alleles by Ad-Cre infection (Supplementary Figure S7A,B).We also confirmed that the Frt-Stop-Frt cassette was deleted from the Runx3 FSF allele in the KPR L/F -TAM(+) cancers, indicating that Runx3 was restored in these cancers (Supplementary Figure S7B)./F mice infected with Ad-Cre.Two weeks after Ad-Cre infection, the mice were fed normal food (KPR L/F -TAM(-), n = 5) or tamoxifen-containing food (KPR L/F -TAM(+), n = 5) for two weeks, followed by normal food in all mice.The median survival of the mice is shown on the right.(F) Schematic representation of the experimental strategy used for establishing KPR -and KPR restored cell lines from lung ADCs developed in KPR L/F -TAM(-) mice.(G) KPR -cells were cultured in the presence or absence of 4-OHT and harvested at the indicated time points.Expression of Runx3 and formation of the R-pointassociated activator (Rpa-RX3-AC) complex was measured using immunoprecipitation (IP) followed by immunoblotting (IB).Induction of Arf expression was measured through IB. (H) The KPR -and KPR restored cell lines were transfected with empty vector (Vec) or p53-expressing plasmids.The expression levels of Runx3, Arf, p53, and cleaved Caspase-3 were detected using IB.

Runx3 Restoration Recovers the R-Point and Induces Arf Expression
We previously reported that RUNX3 plays a key role in R-point regulation by forming the R-point-associated RUNX3-containing activator (Rpa-RX3-AC) complex, which induces ARF expression in response to oncogenic K-RAS activity [20].The Rpa-RX3-AC complex includes RUNX3, p300, BRD2, MLLs, the chromatin remodeling complex (SWI/SNF), and the basal transcription machinery (TFIID).In normal cells, the Rpa-RX3-AC complex is formed only for short intervals (1-2 h) when RAS is activated by mitogenic signals and quickly dissociates as the signal attenuates [20].Then, the cells undergo cell cycle progression.However, in oncogenic RAS-expressing cells, the Rpa-RX3-AC complex is maintained for a long time, as the oncogenic RAS signal is not attenuated.Thus, the Rpa-RX3-AC complex selectively activates the ARF-p53 pathway in response to the aberrant persistence of the RAS signal.However, whether the ARF-p53 pathway is sensitive enough to respond to a persistent low level of oncogenic K-RAS activity originating from heterozygous oncogenic K-RAS mutation remains unclear [13][14][15].To determine whether the pathway responds to heterozygous oncogenic K-Ras mutation, we measured the expression of Arf in KPR L/F -TAM(-) and KPR L/F -TAM(+) lung cancers, which bear a heterozygous oncogenic K-Ras mutation.The results of immunostaining showed that Arf was not expressed in KPR L/F -TAM(-) lung cancers, whereas it was expressed in KPR L/F -TAM(+) lung cancers in which Runx3 was restored (Supplementary Figure S8A).These results suggest that the Arf-p53 pathway is inactivated in the absence of Runx3, whereas it is activated through the restoration of Runx3 in response to heterozygous oncogenic K-Ras mutation.
To determine whether the Rpa-RX3-AC complex formation is also sensitive enough to respond to the heterozygous oncogenic K-Ras mutation, we obtained immortalized cell lines from KPR L/F -TAM(-) lung cancers (KPR -) (Figure 5F).Treatment of KPR -cell lines with 4-hydroxytamoxifen (4-OHT) restored Runx3, generating KPR restored cell lines (Figures 5F and S4C).We confirmed that Runx3 was restored at 8 h after 4-OHT treatment (Figure 5G).Immunoprecipitation (IP) followed by IB showed that the restored Runx3 associated with p300, Brd2, Brg-1 (a component of SWI/SNF), and Tbp (a component of TFIID), indicating the formation of the Rpa-RX3-AC complex (Figure 5G).The Rpa-RX3-AC complex was maintained until 24 h after 4-OHT treatment.Arf was not expressed in KPR -cells, whereas it was expressed in Runx3-restored cells (KPR restored ) (Figure 5G).These results demonstrate that the Rpa-RX3-AC complex is sensitive enough to activate the Arf-p53 pathway in response to a persistent low level of oncogenic K-Ras activity.
The KPR -and KPR restored cell lines were transfected with empty vector or p53-expressing plasmid.Ectopic expression of p53 in the KPR -cells, in which Arf was not expressed, activated Caspase-3 weakly (Figure 5H).Caspase-3 was also weakly activated in the KPR restored cells, in which p53 was deleted and Arf was induced through Runx3 restoration (Figure 5H).However, expression of p53 in KPR restored cells strongly activated Caspase-3 (Figure 5H).Densitometric analysis of the band intensities revealed that the combination of Runx3 restoration and p53 expression resulted in an approximately eight-fold stronger activation of Caspase-3 than either Runx3 restoration or p53 expression alone (Supplementary Figure S8B).These results are consistent with our in vivo observations that the tumor-suppressive activity of Runx3 is largely dependent on p53 activity (Figures 2B and 5D).The results suggest that the R-pointassociated Arf-p53 pathway is abrogated with Runx3 inactivation and recovered with Runx3 restoration in lung cancer cells bearing a heterozygous oncogenic K-Ras mutation.

Runx3 Restoration Inhibits Proliferation of K-Ras-Activated Lung Tumor Cells in a p53-Independent Manner
Although the tumor-suppressive activity of Runx3 was largely dependent on p53, Runx3 restoration extended the survival of KPR L/F mice (Figure 5E).Microscopy revealed that the lung cancers developed in KPR L/F -TAM(-) mice and KPR L/F -TAM(+) mice were pathologically indistinguishable (Supplementary Figure S9).However, the proliferation rate of KPR restored cells was lower than that of KPR -cells (p = 0.003) (Figure 6A).Consistently, the number of PCNA-positive cells in KPR L/F lung tumors was significantly reduced with Runx3 restoration (KPR L/F -TAM(-) = 797.2/mm 2 ; KPR L/F -TAM(+) = 423.5/mm 2 , p = 0.023) (Figure 6B,C).Enlarged microscopic images of Figure 6B are shown in Supplementary Figure S10.These results suggest that Runx3 restoration inhibits the proliferation of K-Rasactivated lung cancer cells.To identify genes regulated by Runx3 in the K-Ras-activated lung cancer cells, we restored To identify genes regulated by Runx3 in the K-Ras-activated lung cancer cells, we performed mRNA sequencing (RNA-seq) in KPR -cells and KPR restored cells.Analysis of the Z-scores revealed that 3,194 and 3,002 genes were induced and suppressed, respectively, in response to Runx3 restoration (Figure 6D).Major signaling pathways upregulated with Runx3 restoration involved apoptosis and negative regulation of proliferation (Figure 6E).On the other hand, genes involved in the positive regulation of cell proliferation and DNA replication were suppressed with RUNX3 expression (Figure 6F).RUNX3-dependent up and downregulated genes involved in apoptosis and negative regulation of proliferation are listed in Supplementary Figure S11.Although Runx3 restoration upregulated the expression of many genes involved in apoptosis, the KPR restored cells did not undergo apoptosis (Figure 5H), suggesting that the Arf-p53 pathway is essential for inducing apoptosis in K-Ras-activated lung cancer cells.Taken together, these results suggest that Runx3 restoration suppresses K-Ras-activated lung cancer mainly through the activation of Arf-p53 pathway-mediated apoptosis and partly through p53-independent inhibition of proliferation.Detailed RNA-seq results are provided in the Excel file (Supplementary Data S2).Further research is required to identify statistically meaningful target genes of the Runx3-induced p53-independent inhibition of proliferation.

Discussion
Rapid recurrence of cancer after treatment with oncogenic K-RAS-specific inhibitors suggests that early tumor lesions are resistant to oncoprotein inhibitors, and that secondary oncogene activation and resistance to the effect of the inhibitors leads to the resumption of cell proliferation [34].ADs that develop without oncogene activation should be resistant to oncoprotein inhibitors.Therefore, to develop new treatment strategies against K-RASactivated lung cancer with a durable response, it is necessary to understand how ADs develop.The ARF-p53 pathway is an effective defense mechanism against oncogenic K-RAS mutations [9,35].Therefore, the development of K-RAS-activated lung ADs must be accompanied by the inactivation of the ARF-p53 pathway.However, oncogenic K-Rasmutated cells develop into lung AD in the absence of p53 mutation [16,36,37].In addition, in a K-Ras-activated mouse lung cancer model, p53 restoration eliminates only ADCs, leaving ADs intact [13,14].These results led to the speculation that the Arf-p53 pathway has inherent limits in its capacity to respond to heterozygous oncogenic K-Ras mutation, and, therefore, oncogenic K-Ras alone is sufficient to induce lung ADs [13][14][15].
However, another possibility was suggested.The initial step of colorectal AD development is the inactivation of adenomatous polyposis coli (APC), and activation of K-Ras occurs after AD development [18,38].In addition, Apc restoration in established Apc-inactivated and K-Ras-activated mouse colorectal ADCs drives rapid and widespread cancer cell differentiation and sustained regression without recurrence [39].These results indicate that mammals have evolved an effective defense mechanism against oncogenic K-Ras mutations, and the mechanism is abrogated in colorectal cancer through Apc inactivation, which induces the formation of colorectal ADs.Lung cancers develop through a similar multistep tumorigenesis pathway (ADs progress into ADCs).We previously reported that inactivation of Runx3 in the mouse lung induces the development of ADs [24,25].In addition, Runx3 inactivation is an earlier event than K-Ras activation in a carcinogen-induced mouse lung cancer model that recapitulates the features of K-RAS-driven human lung cancers [40].Runx3 plays a key role in the R-point decision-making machinery, which senses aberrant oncogenic signals and activates the Arf-p53 pathway [20].Therefore, a single molecular event, the inactivation of Runx3, results in both AD development and the disruption of the Arf-p53 pathway.Indeed, K-Ras activation, with or without p53 inactivation, in an extremely small number of cells failed to induce pathologic lesions for up to 1 year [40].In contrast, Runx3 inactivation and K-Ras activation with the same targeting method led to the rapid induction of lung ADs and ADCs, and it caused lethality in all the targeted mice within 3 months [40].Therefore, Runx3 restoration may regress K-Rasmutated lung tumors and result in sustained regression without recurrence, similar to the effect of Apc restoration.In this study, Runx3 restoration in a mouse lung cancer model regressed K-Ras-activated ADs as well as ADCs, suppressed secondary oncogene activation, and markedly extended the survival of mice (by approximately 15 weeks).Knockdown of oncogenic K-Ras regresses mouse lung cancer; however, the lung cancer recurs after 2 weeks with secondary oncogene activation [8].In this study, Runx3-restored mouse lung cancer did not recur until 10 weeks after regression (14 weeks after Runx3 restoration).The recurred tumor demonstrated spontaneous inactivation of the restored Runx3 allele without secondary oncogene activation.These results show that expression of Runx3 could be helpful for the treatment of lung cancer and for achieving sustained regression.
Arf expression in K-Ras-activated lung cancer was stopped upon Runx3 inactivation and recovered with Runx3 restoration (Figure 5G and Figure S8A).The tumor-suppressive activity of Runx3 was largely dependent on p53 activity, although not completely (Figure 5D).Taken together, these results suggest that Runx3 is an essential upstream regulator of Arf-p53 pathway activation.These observations might explain the rapid recurrence of K-Ras-induced lung cancers with secondary oncogene activation after initial regression due to K-Ras suppression: inhibition of oncogenic K-Ras causes the cancer to regress; however, K-Ras mutation-free AD cells are resistant to the inhibition and are cancerprone because their Arf-p53 pathway (oncogene surveillance mechanism) is suppressed through Runx3 inactivation.In contrast, Runx3 restoration recovers the oncogene surveillance mechanism and could, therefore, lead to inhibition of secondary oncogene activation as well as cancer regression.It has long remained unclear why the Arf-p53 pathway fails to eliminate K-Ras-activated lung ADs [13,14].The present results suggest that p53 restoration failed to regress lung ADs not because the Arf-p53 pathway had an inherent limitation in responding to oncogenic K-Ras activity, but because the pathway was disrupted by Runx3 silencing in lung ADs.This does not explain why the p53 mutation still occurs after K-RAS activation in lung tumorigenesis.The ARF-p53 pathway protects cells from oncogene activation.In contrast, the ATM/ATR-p53 pathway protects cells from genome instability [41].Therefore, p53 mutations at relatively late stages of lung tumorigenesis may be associated with disruption of the ATM/ATR-p53 pathway-mediated defense against genome instability.

Conclusions
Not only in K-RAS-activated lung cancers, but in almost all other malignancies, clinical responses have been yielded through the application of targeted therapies that inhibit activated oncogenes, but despite the application of these therapies, tumor recurrence has eventually resulted [42,43].Therefore, it seems to be of therapeutic value to identify tumor suppressor pathways capable of regressing established cancers and inhibiting cancer recurrence.This study identified Runx3 as such a tumor suppressor that could effectively regress established K-Ras-activated mouse lung cancer and inhibit cancer recurrence.

Figure 2 .
Figure 2. Runx3 restoration regresses K-Ras-activated lung cancers by inducing apoptosis.(A) Ad-Cre-infected KR L/F mice were fed normal food or tamoxifen-containing food for 1 week (KR L/F -TAM(-)-1w and KR L/F -TAM(+)-1w, respectively).Microscopic images of mouse lungs subjected to Tomato and TUNEL staining are shown.Microscopic images of the adjacent sections stained with anti-Runx3, anti-Arf, and anti-p53 are shown on the right.(B,C) Microscopic images of KR L/F -TAM(-)-4w and KR L/F -TAM(+)-4w mouse lungs subjected to HE and TUNEL staining.

Figure 2 .
Figure 2. Runx3 restoration regresses K-Ras-activated lung cancers by inducing apoptosis.(A) Ad-Creinfected KR L/F mice were fed normal food or tamoxifen-containing food for 1 week (KR L/F -TAM(-)-1w and KR L/F -TAM(+)-1w, respectively).Microscopic images of mouse lungs subjected to Tomato and TUNEL staining are shown.Microscopic images of the adjacent sections stained with anti-Runx3, anti-Arf, and anti-p53 are shown on the right.(B,C) Microscopic images of KR L/F -TAM(-)-4w and KR L/F -TAM(+)-4w mouse lungs subjected to HE and TUNEL staining.

Figure 3 .
Figure 3. Lung cancers regressed by Runx3 restoration recur with silencing of the restored Ru allele.(A) Microscopic images of KR L/F -TAM(+)-14w mouse lungs (Figure 2B) subjected to HE a Tomato staining.Magnified images of the boxed regions are shown below.(B) Two control lu ADCs of KR L/F -TAM(-)-0w and four recurred lung ADCs of KR L/F -TAM(+)-14w mice were analy via whole-exon sequencing.Among the known major oncogenes involved in lung cancer, only Ras G12D mutation was detected.Exon mut, number of mutations detected within exons; Total m number of mutations detected within the genome.(C) Targeting of the K-Ras LSL-G12D , Runx3 flox , a Runx3 FSF alleles by Ad-Cre infection followed by tamoxifen treatment in cancers was verif through genomic PCR.(D) Runx3 expression detected with anti-Runx3 antibody (1E10) in lu ADCs developed in KR L/F -TAM(+)-14w mice.Magnified images of the boxed regions are shown.DNA methylation of the Runx3 CpG island detected using MS-PCR in lung ADCs developed KR L/F -TAM(-)-0w mice and four recurred lung ADCs of KR L/F -TAM(+)-14w mice.Runx3-inactiva ADCs produced via DNA methylation are indicated with red letters.M, methylated Runx3 C island; U, unmethylated Runx3 CpG island.

Figure 3 .
Figure 3. Lung cancers regressed by Runx3 restoration recur with silencing of the restored Runx3 allele.(A) Microscopic images of KR L/F -TAM(+)-14w mouse lungs (Figure 2B) subjected to HE and Tomato staining.Magnified images of the boxed regions are shown below.(B) Two control lung ADCs of KR L/F -TAM(-)-0w and four recurred lung ADCs of KR L/F -TAM(+)-14w mice were analyzed via whole-exon sequencing.Among the known major oncogenes involved in lung cancer, only K-Ras G12D mutation was detected.Exon mut, number of mutations detected within exons; Total mut, number of mutations detected within the genome.(C) Targeting of the K-Ras LSL-G12D , Runx3 flox , and Runx3 FSF alleles by Ad-Cre infection followed by tamoxifen treatment in cancers was verified through genomic PCR.(D) Runx3 expression detected with anti-Runx3 antibody (1E10) in lung ADCs developed in KR L/F -TAM(+)-14w mice.Magnified images of the boxed regions are shown.(E) DNA methylation of the Runx3 CpG island detected using MS-PCR in lung ADCs developed in KR L/F -TAM(-)-0w mice and four recurred lung ADCs of KR L/F -TAM(+)-14w mice.Runx3-inactivated ADCs produced via DNA methylation are indicated with red letters.M, methylated Runx3 CpG island; U, unmethylated Runx3 CpG island.

Figure 4 .
Figure 4. Runx3 inactivation is essential for the maintenance of K-Ras-activated lung cancer.(A)Schematic representation of the experimental strategy used for examining the role of Runx3 in the maintenance of mouse lung cancer induced through K-Ras-activation alone.The K LA1 R FSF/+ mice bear the K-Ras LA1/+ , Runx3 FSF/+ , and Flp ERT2 alleles.In the K LA1 R FSF/+ mice, K-Ras was activated through spontaneous recombination.Treatment with tamoxifen (TAM) restored one allele of Runx3 by activating Flp ERT2 , which deleted the Frt-Stop-Frt cassette from the Runx3 FSF allele.(B) Survival curves of K LA1 R +/+ mice and K LA1 R FSF/+ mice infected with Ad-Cre.Ten weeks after birth, the K LA1 R FSF/+ mice were fed normal food (K LA1 R FSF/+ -TAM(-), n = 15) or tamoxifen-containing food (K LA1 R FSF/+ -TAM(+), n = 15) for two weeks, followed by normal food in all the mice.(C) Statistical analysis of the lifespan of K LA1 R +/+ mice and K LA1 R FSF/+ mice treated with or without tamoxifen.(D,E) DNA methylation of the Runx3 CpG island detected through MS-PCR in lung ADCs developed in 50-week-old K LA1 R FSF/+ -TAM(-) mice and K LA1 R FSF/+ -TAM(+) mice.M, methylated Runx3 CpG island; U, unmethylated Runx3 CpG island.(F) Runx3 expression detected with anti-Runx3 antibody (1E10) in lung ADCs

Figure 4 .
Figure 4. Runx3 inactivation is essential for the maintenance of K-Ras-activated lung cancer.(A)Schematic representation of the experimental strategy used for examining the role of Runx3 in the maintenance of mouse lung cancer induced through K-Ras-activation alone.The K LA1 R FSF/+ mice bear the K-Ras LA1/+ , Runx3 FSF/+ , and Flp ERT2 alleles.In the K LA1 R FSF/+ mice, K-Ras was activated through spontaneous recombination.Treatment with tamoxifen (TAM) restored one allele of Runx3 by activating Flp ERT2 , which deleted the Frt-Stop-Frt cassette from the Runx3 FSF allele.(B) Survival curves of K LA1 R +/+ mice and K LA1 R FSF/+ mice infected with Ad-Cre.Ten weeks after birth, the K LA1 R FSF/+ mice were fed normal food (K LA1 R FSF/+ -TAM(-), n = 15) or tamoxifen-containing food (K LA1 R FSF/+ -TAM(+), n = 15) for two weeks, followed by normal food in all the mice.(C) Statistical analysis of the lifespan of K LA1 R +/+ mice and K LA1 R FSF/+ mice treated with or without tamoxifen.(D,E) DNA methylation of the Runx3 CpG island detected through MS-PCR in lung ADCs developed in 50-week-old K LA1 R FSF/+ -TAM(-) mice and K LA1 R FSF/+ -TAM(+) mice.M, methylated Runx3 CpG

Figure 5 .
Figure 5. Runx3 restoration eliminates lung cancers through the Arf-p53 pathway.(A) Schematic representation of the experimental strategy used for examining the effect of Runx3 restoration in KPR L/F mice.The KPR L/F mice bear the Rosa26R-Tomato (Tomato*), K-Ras LoxP-Stop-LoxP-G12D/+ (K-Ras LSL- G12D/+ ), p53 flox/flox , Runx3 flox/FSF , and Flp ERT2 alleles.In the KPR L/F mice, Ad-Cre infection activated K-Ras, deactivated p53 and Runx3, and induced lung ADs/ADCs.Treatment with tamoxifen (TAM) restored Runx3 by activating Flp ERT2 , which deleted the Frt-Stop-Frt cassette from the Runx3 FSF allele.(B) Gross images of Tomato fluorescence emitted under UV light from the lungs of KPR L/F mice 2 weeks after Ad-Cre infection.(C) Microscopic images of lung tumors developed in KR L/F mice and KPR L/F mice 2 weeks after Ad-Cre infection.Lung tumors subjected to HE staining are shown.(D) Survival curves of KR L/F mice and KPR L/F mice infected with Ad-Cre.The median survival of the mice is shown on the right.p = p-value.(E) Survival curve of KPR L/F mice infected with Ad-Cre.Two weeks after Ad-Cre infection, the mice were fed normal food (KPR L/F -TAM(-), n = 5) or tamoxifen-containing food (KPR L/F -TAM(+), n = 5) for two weeks, followed by normal food in all mice.The median survival of the mice is shown on the right.(F) Schematic representation of the experimental strategy used for establishing KPR -and KPR restored cell lines from lung ADCs developed in KPR L/F -TAM(-) mice.(G) KPR -cells were cultured in the presence or absence of 4-OHT and harvested at the

Figure 5 .
Figure 5. Runx3 restoration eliminates lung cancers through the Arf-p53 pathway.(A) Schematic representation of the experimental strategy used for examining the effect of Runx3 restoration in KPR L/F mice.The KPR L/F mice bear the Rosa26R-Tomato (Tomato*), K-Ras LoxP-Stop-LoxP-G12D/+ (K-Ras LSL-G12D/+ ), p53 flox/flox , Runx3 flox/FSF , and Flp ERT2 alleles.In the KPR L/F mice, Ad-Cre infection activated K-Ras, deactivated p53 and Runx3, and induced lung ADs/ADCs.Treatment with tamoxifen (TAM) restored Runx3 by activating Flp ERT2 , which deleted the Frt-Stop-Frt cassette from the Runx3 FSF allele.(B) Gross images of Tomato fluorescence emitted under UV light from the lungs of KPR L/F mice 2 weeks after Ad-Cre infection.(C) Microscopic images of lung tumors developed in KR L/F mice and KPR L/F mice 2 weeks after Ad-Cre infection.Lung tumors subjected to HE staining are shown.(D) Survival curves of KR L/F mice and KPR L/F mice infected with Ad-Cre.The median survival of the mice is shown on the right.p = p-value.(E) Survival curve of KPR L/F mice infected with Ad-Cre.Two weeks after Ad-Cre infection, the mice were fed normal food (KPR L/F -TAM(-), n = 5) or tamoxifen-containing food (KPR L/F -TAM(+), n = 5) for two weeks, followed by normal food in all mice.The median survival of the mice is shown on the right.(F) Schematic representation of the experimental strategy used for establishing KPR -and KPR restored cell lines from lung ADCs developed in KPR L/F -TAM(-) mice.(G) KPR -cells were cultured in the presence or absence of 4-OHT

Cells 2023 , 20 Figure 6 .
Figure 6.Runx3 restoration inhibits the proliferation of lung ADC cells.(A) KPR -and KPR restored cells were cultured and the cell numbers were analyzed.The average numbers of KPR -and KPR restored cells 3 days after culture are shown on the right (p = p-value).(B) Microscopic images of KPR L/F -TAM(-)-4w and KPR L/F -TAM(+)-4w mouse lungs subjected to HE, PCNA, and Tomato staining.(C) The average numbers of PCNA-positive cells per mm² of cancer regions.(D) Heatmap showing genes up or downregulated by Runx3 restoration.The fold change of each gene was converted to the log2 value to generate the heatmap.(E,F) The major signaling categories of upregulated and downregulated genes in Runx3 restoration are shown.The numbers in the brackets indicate the number of genes.

Figure 6 .
Figure 6.Runx3 restoration inhibits the proliferation of lung ADC cells.(A) KPR -and KPR restored cells were cultured and the cell numbers were analyzed.The average numbers of KPR -and KPR restored cells 3 days after culture are shown on the right (p = p-value).(B) Microscopic images of KPR L/F -TAM(-)-4w and KPR L/F -TAM(+)-4w mouse lungs subjected to HE, PCNA, and Tomato staining.(C) The average numbers of PCNA-positive cells per mm 2 of cancer regions.(D) Heatmap showing genes up or downregulated by Runx3 restoration.The fold change of each gene was converted to the log 2 value to generate the heatmap.(E,F) The major signaling categories of upregulated and downregulated genes in Runx3 restoration are shown.The numbers in the brackets indicate the number of genes.

:
Generation of Runx3 FSF/+ mice; Figure S2: Enlarged microscopic images of Figure 1C-G; Figure S3: Targeting of the K-Ras LSL-G12D , Runx3 flox , and Runx3 FSF alleles by Ad-Cre or tamoxifen in lung tumors developed in KR L/F mice; Figure S4: Enlarged microscopic images of Figure 2A; Figure S5: Enlarged microscopic images of Figure 2A; Figure S6: Nucleotide sequence of the Runx3 CpG island subjected to MS-PCR; Figure S7: Targeting of the Kras LSL-G12D , p53 flox , Runx3 flox , and Runx3 FSF alleles by Ad-Cre or tamoxifen in lung cancers developed in KPR L/F mice; Figure S8: Induction of Arf by Runx3 restoration in KR L/F -TAM(+) mouse lung cancers; Figure S9: The tumors developed in KPR L/F -TAM(-) mice and KPR L/F -TAM(+) mice were pathologically indistinguishable; Figure S10: Enlarged microscopic images of Figure 6B; Figure S11: Genes up or downregulated by Runx3 restoration in KPR cells; Data S1: Exon sequencing; Data S2: mRNA sequencing.