Microscopic Description of Platelet Aggregates Induced by Escherichia coli Strains
Abstract
1. Introduction
2. Materials and Methods
2.1. Preparation of the Washed Platelets
2.2. Preparation of Bacteria
2.3. Scanning Electron Microscopy (SEM) of Whole Platelet-Bacteria Aggregates
2.4. SEM of Ultra-Thin Sections of Platelet-Bacteria Aggregates
2.5. Transmission Electron Microscopy (TEM) of Negatively Stained Bacteria
2.6. SEM of Whole Bacteria
2.7. Statistical Analysis
3. Results
3.1. SEM of Whole Platelet-Bacteria Mixtures
3.2. SEM of Ultra-Thin Sections of Platelet-Bacteria Mixtures
3.3. Electron Microscopy of Bacteria
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strain | Origin | Platelet Activation | Platelet Bactericidal Effect | O-Antigen Serotyping | Reference |
---|---|---|---|---|---|
K12 | Laboratory strain | + | - | - | [9] |
LH30 | Clinical isolate | - | - | O8 | [9] |
J53 | Laboratory strain | + | + | O16 | [9] |
BSE-SEM of Whole Platelets-Bacteria Aggregates (Figure 1) | BSE-SEM of Ultrathin Sections of Platelets-Bacteria Aggregates (Figure 2) | ||||||
---|---|---|---|---|---|---|---|
Criteria | Visible Platelets | Visible Bacteria | Platelet Activation | Platelet Integrity | Platelet Activation | Platelet Granules | Bacteria’s Location Regarding Platelets |
K12 strain | Yes | Yes | Moderate | Yes | Moderate | Inside platelets | Mixed |
LH30 strain | Yes | Yes | No | Yes | No | Inside platelets | Side by side |
J53 strain | Moderate | No | NC | Amorphous matrix | High | Among the amorphous matrix | Inside the amorphous matrix |
E. coli Strain | BSE-SEM Ultrathin Sections (Figure 3) | TEM Negative Staining (Figure 4) | SE-SEM (Figure 4) | |
---|---|---|---|---|
Technique | ||||
K12 | Regular cell wall, attached to periplasm, electron-dense bodies | Elongated shape | Thin surface reliefs | |
LH30 | Irregular shaped cell wall, detached from periplasm, electron-dense periplasm | Elongated shape | Thick surface reliefs | |
J53 | Sinuous cell wall, detached from periplasm, electron-dense periplasm | Elongated shape ± flagella | Thin surface reliefs |
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Ezzeroug Ezzraimi, A.; Baudoin, J.-P.; Mariotti, A.; Camoin-Jau, L. Microscopic Description of Platelet Aggregates Induced by Escherichia coli Strains. Cells 2022, 11, 3495. https://doi.org/10.3390/cells11213495
Ezzeroug Ezzraimi A, Baudoin J-P, Mariotti A, Camoin-Jau L. Microscopic Description of Platelet Aggregates Induced by Escherichia coli Strains. Cells. 2022; 11(21):3495. https://doi.org/10.3390/cells11213495
Chicago/Turabian StyleEzzeroug Ezzraimi, Amina, Jean-Pierre Baudoin, Antoine Mariotti, and Laurence Camoin-Jau. 2022. "Microscopic Description of Platelet Aggregates Induced by Escherichia coli Strains" Cells 11, no. 21: 3495. https://doi.org/10.3390/cells11213495
APA StyleEzzeroug Ezzraimi, A., Baudoin, J.-P., Mariotti, A., & Camoin-Jau, L. (2022). Microscopic Description of Platelet Aggregates Induced by Escherichia coli Strains. Cells, 11(21), 3495. https://doi.org/10.3390/cells11213495