Production , Leaf Quality and Antioxidants of Perennial Wall Rocket as A ff ected by Crop Cycle and Mulching Type

The plastic mulch has raised a disposal issue, which has been diverting the research focus on biodegradable film as an alternative. Research was carried out in southern Italy in 2016–2017 and 2017–2018 in order to assess the effects of three crop cycles (autumn-winter, winter, spring) in factorial combination with three soil mulching types (a MaterBi biodegradable black film; a brown photoselective low density polyethylene (LDPE) film; a black-standard LDPE film) and a non-mulched control, on leaves yield, quality and antioxidants of greenhouse grown Diplotaxis tenuifolia (L.) D.C. The spring cycle was the shortest and best enhanced plant growth and yield. The non-mulched control caused an 11% yield reduction compared to the mulching treatments average (12.4 t ha−1). The soil temperature was highest under photoselective and standard LDPE films. The Soil Plant Analysis Development (SPAD) index was 17.4% higher in the leaves grown in mulched soil. Winter season and biodegradable mulch led to higher leaf dry residue and organic acids. Leaf nitrate content was highest in winter and under mulching. The spring cycle, the biodegradable and photoselective LDPE film resulted in the highest antioxidant compound content and activity. The biodegradable polymer improved leaf quality, showing suitable features for sustainable production.


Introduction
Perennial wall rocket (Diplotaxis tenuifolia (L.) D.C.) belongs to the Brassicaceae family, is currently cultivated in several agricultural areas worldwide and, in particular, on about 4800 ha in Italy based on the latest estimation in 2018 [1].The increasing diffusion of perennial wall rocket in the last two decades, both as a fresh-market and ready-to-use salad oriented crop, is due to its smooth and succulent leaves which meet the expectations of consumers.The leaves of this species are also rich in mineral elements and antioxidants [2], the latter protecting against ultraviolet radiations [3], though their excessive content may be averse to lipid, protein and nucleic acid stability [4].
When the produce is addressed to fresh market, the crop cycles of perennial wall rocket are carried out in sequence from autumn to spring or from spring to summer, the cultivation season showing significant effects on yield and quality performances of D. tenuifolia [5] as well as of other vegetable species [6][7][8].The transplant is usually practiced on mulched ridges, as the soil cover allows for a reduction of crop duration, a better weed management and an improved produce quality.
Up to date, plastic material (usually polyethylene) is mostly used for soil mulching worldwide, though biodegradable films have slowly been increasing and currently account for almost 5% out of the total mulched surface area [9].However, the use of plastic represents a spotlighted issue due to the adverse environmental impact connected to its disposal.In this respect, the agricultural plastic waste on the land is being estimated and mapped by using satellite images, with the aim to monitor and optimize its collection from the farms and their transport to the recycling companies [10,11]; notably, in Barletta-Andria-Trani Province of Apulia Region the agricultural plastic waste amounts to 1.2 tons per hectare [12].Indeed, plastic degradation is a complex process which is remarkably affected by the plastic composition as well as the biotic and abiotic conditions they are exposed to [13].In this respect, the biodegradable polymers offer the chance to make a sustainable choice from the environmental and crop management point of view [14,15].Indeed, biodegradable films are incorporated directly into the soil and decomposed by microorganisms, eventually into CO 2 and H 2 O [16].However, the rate of biodegradation depends on different factors such as polymer characteristics (even thickness), soil and environmental conditions [13].In this respect, the films can break down too slowly, resulting in problems with persistent small fragments of residual mulch, or too quickly, leaving the crop unprotected [17].The economic benefit connected to the use of biodegradable mulch may be slightly lower than the plastic one due to the higher current cost of biodegradable films [18], which however do not need to be removed from the soil.Mater-Bi is a starch-based biopolymer which is similar to traditional plastic in terms of agronomic characteristics and to cellulose referring to degradation rate [19].Mater-Bi black mulch also showed good performances in organic system under the Mediterranean continental climate [20].
A scientific investigation aimed to assess the effects of biodegradable mulch on yield, quality and antioxidant properties of perennial wall rocket leaves was carried out in southern Italy, in order to provide useful details for the cost evaluation of these materials at farm scale, taking into account they are incorporated into soil after their use, and therefore are costless for removal and disposal.In this respect, a biodegradable mulch was compared with a standard and a photoselective LDPE, in interaction with three crop cycles.

General Analytical Methods
Research on rocket (Diplotaxis tenuifolia (L.) D.C.) cultivar Nature was carried out in Portici (Naples, southern Italy) in 2016-2017 and 2017-2018 on a clay-sandy soil; in a three-span polytunnel with each span being 5.0 m wide, 2.0 and 3.5 m tall at wall and roof respectively.The trend of temperature is shown in Figure 1 as mean values of the two research years, because no variable regarding the plant determinations was affected by the year of investigation.The experimental protocol was based on the comparison between three crop cycles in factorial combination with three soil mulching types and a non-mulched control.The three crop cycles corresponded to the following seasons: autumn-winter; winter; spring.The three mulching types were: a MaterBi biodegradable black film (Biodegradable), 15 µm thick EF04P made of corn starch by Novamont S.p.A., Novara, Italy; a brown photoselective light low density polyethylene film (Photoselective LDPE), 25 µm thick Al-Or C-889 by PolyEur S.p.A., which has an 80% transmissivity in the near infrared range (from 780 nm to 2500 nm) and absorbs 96% of the photosynthetically active radiations (PAR); a standard black low light density polyethylene film (Standard LDPE), 45 µm thick.The thicknesses of the three mulch films are different because they are referred to diverse materials, and each of them entails a specific goal: the 45 µm standard LDPE is the commonly used type in Italian vegetable systems, because smaller thickness may result in the material break down; the biodegradable film cannot be as thick as the standard LDPE, because it would show difficulties to be degraded in that case; the photoselective LDPE is aimed to transmit near IR radiations and, therefore, it is thinner than the standard LDPE.A randomized complete block design was used with three replicates and the elementary plot had a 3.2 m 2 surface area.
Agronomy 2019, 9, x FOR PEER REVIEW 3 of 18 randomized complete block design was used with three replicates and the elementary plot had a 3.2 m 2 surface area.The crops were managed by performing environmentally sustainable farming practices.Prior to transplant, the soil was ploughed and hoed, organically fertilized with 38 kg•ha −1 N, 10 P2O5, 30 K2O, arranged in 100 cm wide raised beds which were mulched according to the experimental protocol.The transplant was performed on 16 and 20 November in 2016 and 2017, respectively with plants spaced 20 cm along and between the rows within each bed, with 80 cm between the outer rows of adjacent beds (14.3 alveoli per m 2 ).The following practices were done during the growing season: 112 kg•ha −1 N 30 P2O5, and 90 K2O supply through fertigation; hoeing; drip irrigation when the soil available water capacity in the first 20 cm of soil profile decreased to 80%; six foliar spraying applications (two in the first crop cycle, three in the second and one in the third) for plant protection against fungal diseases and insects using copper (0.7 kg•ha -1 copper oxichloride) and azadirachtin (25 mL•ha −1 active ingredient).
Harvests of commercially ripe leaves (12 to 15 cm length) were performed, practicing the cut at 3 to 5 cm above the cotyledons in order to allow for efficient vegetative apex regrowth [21], in the following dates: 28 and 31 January in 2017 and 2018, respectively, for the first crop cycle; on 14 and 16 March in 2017 and 2018, respectively, for the second crop cycle; on 19 April both in 2017 and 2018, for the third crop cycle.
The soil temperature (Figure 2) at 10 cm depth, which is the average depth of perennial wall rocket plant roots in this research crop system, was measured every hour in each plot during the three crop cycles, using PT100 sensors connected to the Console Wireless Vantage Pro2, equipped with a data logger (Davis Instruments, Illinois, USA).
At the end of each crop cycle, random plant samples were collected in each plot in order to assess: The total leaf area, using a bench top electronic leaf area meter (Li-Cor3000, Li-Cor, Lincoln, NE, USA); the aboveground dry biomass in an oven at 70 °C until constant weight.
At each harvest, the weight and number of marketable leaves, as well as of leaf mean weight on 100 unit samples were determined in each plot.At the end of both the winter and spring crop cycle, leaf samples per each experimental treatment were taken and transferred to laboratory in order to perform the following determinations.The crops were managed by performing environmentally sustainable farming practices.Prior to transplant, the soil was ploughed and hoed, organically fertilized with 38 kg•ha −1 N, 10 P 2 O 5 , 30 K 2 O, arranged in 100 cm wide raised beds which were mulched according to the experimental protocol.The transplant was performed on 16 and 20 November in 2016 and 2017, respectively with plants spaced 20 cm along and between the rows within each bed, with 80 cm between the outer rows of adjacent beds (14.3 alveoli per m 2 ).The following practices were done during the growing season: 112 kg•ha −1 N 30 P 2 O 5 , and 90 K 2 O supply through fertigation; hoeing; drip irrigation when the soil available water capacity in the first 20 cm of soil profile decreased to 80%; six foliar spraying applications (two in the first crop cycle, three in the second and one in the third) for plant protection against fungal diseases and insects using copper (0.7 kg•ha −1 copper oxichloride) and azadirachtin (25 mL•ha −1 active ingredient).
Harvests of commercially ripe leaves (12 to 15 cm length) were performed, practicing the cut at 3 to 5 cm above the cotyledons in order to allow for efficient vegetative apex regrowth [21], in the following dates: 28 and 31 January in 2017 and 2018, respectively, for the first crop cycle; on 14 and 16 March in 2017 and 2018, respectively, for the second crop cycle; on 19 April both in 2017 and 2018, for the third crop cycle.
The soil temperature (Figure 2) at 10 cm depth, which is the average depth of perennial wall rocket plant roots in this research crop system, was measured every hour in each plot during the three crop cycles, using PT100 sensors connected to the Console Wireless Vantage Pro2, equipped with a data logger (Davis Instruments, Illinois, USA).
At the end of each crop cycle, random plant samples were collected in each plot in order to assess: The total leaf area, using a bench top electronic leaf area meter (Li-Cor3000, Li-Cor, Lincoln, NE, USA); the aboveground dry biomass in an oven at 70 • C until constant weight.
At each harvest, the weight and number of marketable leaves, as well as of leaf mean weight on 100 unit samples were determined in each plot.At the end of both the winter and spring crop cycle, leaf samples per each experimental treatment were taken and transferred to laboratory in order to perform the following determinations.

SPAD and Leaf Colour Parameters
Just before harvesting, the following measurements were performed as previously described [22]: The Soil Plant Analysis Development (SPAD) index, on twenty undamaged rocket leaves per experimental treatment, by means of a portable chlorophyll meter SPAD-502 (Konica Minolta, Tokyo, Japan); the color parameters L* (lightness, from 0 to 100, i.e., black to white), a* and b* (chroma components from -60 to +60, i.e., from green to red and from blue to yellow for 'a' and 'b' respectively).

Dry Matter
The leaf dry matter was assessed after dehydration of the fresh samples in a forced-air oven at 70 °C until constant weight.

SPAD and Leaf Colour Parameters
Just before harvesting, the following measurements were performed as previously described [22]: The Soil Plant Analysis Development (SPAD) index, on twenty undamaged rocket leaves per experimental treatment, by means of a portable chlorophyll meter SPAD-502 (Konica Minolta, Tokyo, Japan); the color parameters L* (lightness, from 0 to 100, i.e., black to white), a* and b* (chroma components from −60 to +60, i.e., from green to red and from blue to yellow for 'a' and 'b' respectively).

Dry Matter
The leaf dry matter was assessed after dehydration of the fresh samples in a forced-air oven at 70 • C until constant weight.

Mineral Elements
The content of NO 3 -N, P, K, Ca, Mg, Na, S and Cl was assessed in leaf dry tissues ground in a Wiley Mill and then sieved through an 841-microns riddle.For this purpose, 250 mg of leaf tissue powder suspended in 50 mL of ultrapure water (Milli-Q, Merck Millipore, Darmstadt, Germany) underwent three freeze-thaw cycles in liquid nitrogen followed by shaking water bath (ShakeTemp SW22, Julabo, Seelbach, Germany) at 80 • C for 10 min.The mixture obtained was managed according to the procedure by Rouphael et al. [23] and the determinations of the mentioned mineral elements were performed as described by Rouphael et al. [22].

Phenols
The total phenols content in methanolic extracts was assessed using the Folin-Ciocalteu method [24] with gallic acid as a standard.For this purpose, 100 mL of the supernatant were combined with 500 mL of Folin-Ciocalteau's reagent (Sigma-Aldrich Inc., Milano, Italy) and 400 mL of 7.5% sodium carbonate/water (w/v).The solution absorbance was measured after 30 min at 765 nm by an ultraviolet-visible spectrophotometer, expressing the results as mg gallic acid (Sigma-Aldrich Inc.) per 100 g of dry weight.

Ascorbic Acid
The total ascorbic acid was assessed by spectrophotometric detection as described by Kampfenkel et al. [25], by reducing the dehydroascorbate to ascorbic acid upon the sample pre-incubation with dithiothreitol.The solution absorbance was measured at 525 nm, expressing the results as mg ascorbic acid per 100 g fresh weight.

Antioxidant Activity
The total antioxidant activity was determined by the 1,1-diphenyl-2-picryl-hydrazil (DPPH) test, following the method of Brand-Williams et al. [26], measuring the absorbance decrease at 515 nm wavelength of a 63.4 µM DPPH solution, using 10 µl of extract.The values obtained were interpolated with those from a calibration line built up using Trolox as a reference antioxidant and the results were expressed as mmol Trolox equivalents (TE) per g of fresh weight.

Statistical Processing
The two-way analysis of variance and the Duncan multiple range test were used for processing the data and performing the mean separations at the 0.05 probability level respectively, by using SPSS software version 21.The angular transformation was applied to percentage data before processing.The variables regarding the plant determinations examined in our research were not significantly affected by the research year and, therefore, only mean data of the two years are reported.Moreover, no significant interactions arose between the two experimental factors "crop cycle" and "mulching type" and for this reason only the data relevant to their main effects are showed.

Precocity, Plant Growth and Yield
Rocket earliness, growth and yield parameters resulting from the comparison between the three crop cycles and the four mulching treatments tested are reported in Table 1.The autumn-winter crop cycle was the longest (73 days), showing more than double duration compared to the shortest spring one (35 days).The spring cycle was associated to the most enhanced plant growth, in terms of leaf expansion and dry matter, as well as yield; the latter was the result of the highest mean weight, though the number of leaves was lowest.Conversely, the winter crop cycle produced the highest number of leaves with the lowest mean weight and the yield was not significant different from that corresponding to the autumn-winter crops.The highest yield performance of the spring crops is associated to the most favorable trend of air temperature inside the greenhouse: The temperature in April was always over 20 • C, whereas it dropped just four times below 5 • C in the second half of March.Consistently with other results, Bonasia et al. [27] recorded higher leaf yield of perennial wall-rocket in winter-spring cycle than in autumn-winter (2.25 vs. 1.50 kg m −2 ).Schiattone et al. [21] reported a marketable yield range between 5 and 18 t ha −1 per growing cycle.
As for the comparison between the four mulching treatments (Table 1), no significant differences of average harvest precocity were recorded between the biodegradable, photoselective and LDPE films (49 days on average), whereas the non-mulched control caused the longest cycle (55 days).Consistently with our results, Ibarra et al. [28] found a positive correlation between the crop earliness and the soil heat accumulation under mulching treatments and no significant differences between the plastic and biodegradable mulch.
The biodegradable and photoselective films did not significantly differ from LPDE in terms of growth and yield (12.4 t ha −1 as an average); indeed, the traditional plastic mulch resulted in lower dry biomass than that produced by the other two materials.The non-mulched control showed smaller plants and an 11% yield reduction due to the lower number of leaves, whereas their mean weight was not significantly affected by mulching treatment.
In our research, the effects of the biodegradable and photoselective films on yield were not significantly different from LDPE, though the thickness of the latter material is more than triple (45 vs. 15 µm).This mainly suggests that the biodegradable mulch is a good environmentally friendly alternative to the plastic one for managing the perennial wall rocket crop in greenhouse; however, all the mulching treatments encouraged the production compared to bare soil, which highlights the importance of both increasing soil temperature and controlling weeds with no costly manual intervention [29].Indeed, in our investigation both the maximum and minimum soil temperatures recorded over the whole crop cycles (Figure 2) were lowest in the non-mulched control and highest under photoselective LDPE and standard LDPE film, maybe also due to the higher thickness compared to the biodegradable mulch, in agreement with previous studies [30].In contrast with our findings relevant to rocket whose leaves do not cover all the soil surface until the end of crop cycle, in previous investigations carried out on open-air grown lettuce [31] and potato [32] the effect of mulch on soil temperature decreased with the full establishment of the plant canopy in the later growth stage.Ngouajio et al. [33] found that the biodegradable mulch led to higher soil temperatures than the polyethylene film in early crop development in open-air conditions.By contrast, Rangarajan and Ingall [34] recorded slightly lower soil temperatures under biodegradable green or black mulch compared to the same colors of polyethylene material.
In all treatments of our research the mean values of soil root-zone temperature fell in the 2 • C to 25 • C range, fitting rocket requirements [2] and thus enhancing physiological processes, such as uptake of water and mineral nutrients, and accordingly growth and yield.However, the maximum soil temperature under standard and photoselective LDPE exceeded 25 • C for an average of seven days in April and this overheating may have damaged the crop.
The differences in the soil temperature among the different mulch types could mainly be attributable to their composition [35].Notably, the highest soil temperatures reached under LDPE may result from the optical properties of this material, which reflects or transmits less than 10% of solar radiation and absorbs the remaining over 90% fraction [36].Indeed, if there is a good contact between soil and mulch, significant heat conduction can occur, thus increasing soil temperature during the daytime [37].The increase of mulch thickness may result in soil temperature rise [38], but it may even not affect the soil heating [33].The biodegradable mulch resulted on average in lower soil temperatures than LDPE and photoselective film: 0.7 • C compared to both plastic mulches referring to the minimum temperatures; 0.6 and 1.0 • C lower than photoselective LDPE and standard LDPE film respectively, regarding maximum temperatures.This is due to the higher permeability of the biodegradable film tested, which encourages gas exchange with the open air [35,39].The latter feature, in combination with the soil overheating caused by LDPE mulch for seven days of the spring crop cycle, referring to perennial wall rocket optimal requirements, may explain the not different yield between the two plastic mulches and the biodegradable one.The lowest bare soil temperature is the result of the highest heat loss upon reflectance and evaporation [37].The highest minimum soil temperatures recorded under LDPE may be explained by the more effective heat accumulation of this material during the day, though the energy loss overnight was higher than the biodegradable mulch as witnessed by the more amplified day-night temperature difference [36,37].
All mulching types reduced the soil temperature fluctuation as compared to bare soil, due to minimum soil temperature increase, as also described by Moreno et al. [30].Consistently with our plant growth and yield results, other authors [18] reported the positive effect of increasing soil temperature under mulching on peanut leaf area index and pod production compared with the bare soil.The 15% starch-based biodegradable film warmed the soil up with similar effectiveness as PE film in the first two months of peanut cultivation, thereafter the soil temperature under the biodegradable mulch was 0.8-2.8• C lower compared to PE at 10 cm soil depth.However, no significant difference in terms of leaf area expansion, SPAD index and yield was recorded between the two mulching types, though the number of pods per plant was higher under the plastic mulch.In research carried out on winter oilseed rape [40], the starch-based biodegradable much did not degrade in winter but only in the late spring crop stages.Accordingly, soil temperature differences between plastic and biodegradable mulch were recorded only in the advanced plant development, when the more moderate temperature under the biodegradable mulch resulted in higher tap root length and lateral root mass density in the 20-30 cm layer.Nevertheless, the seed production was not significantly affected by mulching type.In a study carried out on lettuce [41], biodegradable mulching based on hydrolyzed proteins resulted in lower soil temperatures but higher electrical conductivity than those determined by LDPE film, the difference magnitude depending on biodegradable material tested.However, mulching type showed no significant effect on SPAD index, leaf expansion and dry matter.As reported by Waterer [42], no significant differences in soil temperature were recorded between standard and biodegradable mulch and, accordingly, yields of pepper, zucchini, sweet corn, eggplant and melon were not affected by the type of film.Within each column, n.s.no statistically significant difference; means followed by different letters are significantly different according to the Duncan test at p ≤ 0.05.

Leaf Colour, SPAD Index, Quality and Chemical Composition
The SPAD index was not affected by the crop cycle, whereas the color components a* and b* attained higher values in winter and in spring, respectively.The SPAD index was higher in the leaves grown under mulching (by 17.4% as an average) compared to bare soil (Table 2).All the leaf color components were highest under the biodegradable mulch.
The higher SPAD index observed in rocket plants grown in mulched soil can be associated to the effective enhancement of N uptake efficiency achieved with this farming practice.In fact, the SPAD index, used as a non-invasive and non-destructive estimate of chlorophyll content, is considered a key indicator of the efficiency of chlorophyll biosynthesis and photosynthetic apparatus, which is connected with the crop performance [43].
In previous research [18], the SPAD index under mulching was higher than that recorded in bare soil grown plants; no significant differences were recorded between the 15% starch biodegradable film and the PE film from the pod-setting to pod maturity stage.The SPAD index is generally correlated to chlorophyll content and leaf nitrogen status [44]; in this respect, nitrogen uptake is stimulated by the increase of daylight soil temperature, contrary to the trend of phosphorus and calcium in leaf tissues [45].However, Sartore et al. [41] reported that at harvest leaves grown in soil mulched with protein hydrolysate combined with polyethylene glycol diglycidyl ether (PH-PEG-ESO) showed a tendency to higher SPAD index compared to epoxidized soybean oil (PH-ESO) and LDPE, though the latter mulch generally showed a higher soil temperature than PH-PEG-ESO.
As reported in Table 3, the leaves harvested at mid-March showed higher values of dry residue, oxalate and isocitrate, whereas malate and citrate concentration did not significantly differ between the winter and spring cycle.As for the comparison between the mulch types, the biodegradable mulch resulted in the highest values of dry residue, though not different from the photoselective LDPE film, and of the organic acids concentration which did not differ from the non-mulched control.
As for the mineral composition (Table 4), potassium and chlorine attained higher concentration in the leaves produced in spring, whereas the nitrate content was higher in winter; no significant differences in the other elements analyzed were recorded between the two crop cycles.Consistently with our results, Caruso et al. [5] reported a higher nitrate concentration in perennial wall rocket leaves grown in autumn-winter cycle compared to winter-spring season.
With regard to the comparison between the mulching treatments, Mg, Na and P were most concentrated in the leaves produced with the biodegradable film, whereas the nitrate accumulation was enhanced by mulching independently on the material type.The bare soil caused the lowest leaf content of the aforementioned elements, not differing from the photoselective LDPE film in Na concentration and even from standard LDPE mulch in Mg and P.

Leaf Antioxidants
As for antioxidants (Table 5), the spring harvested leaves of perennial wall rocket showed higher concentration of phenols and ascorbic acid as well as of lipophilic and hydrophilic antioxidant activities compared to the winter grown leaves.
With regard to the comparison between the mulches, the biodegradable and photoselective LDPE films showed the highest values of the antioxidant compounds and activities in D. tenuifolia leaves; however, the effect of the photoselective LDPE material was not significantly different from the standard LDPE referring to phenols content and lipophilic antioxidant activity.
In the present research the ascorbic acid content in perennial wall rocket leaves was 36.7 mg 100 g −1 on average, compared to the average content of 9 mg 100 g −1 reported in literature [2].In this respect, the recommended daily intake for vitamin C is 30-60 mg [46] and, therefore, 123 g per day of wall rocket leaves produced in the present investigation are needed to fulfill the daily requirement of this antioxidant.
Consistently with the findings of the present research, Morra et al. [47] found that polyphenols and other antioxidant compounds increased in strawberry fruits grown in soil mulched with MaterBi 20 µm thick biodegradable film in comparison to LDPE.Unlike the results of our investigation, Sartore et al. [41] reported that the total polyphenols concentration in lettuce leaves did not differ between the two protein hydrolyzate-based mulches tested (with the addition of ESO or PEG-ESO), but under both materials the content was 17% lower compared to LDPE; ascorbic acid showed the same trend and was 11% lower for the two spray treatments than for the LDPE mulch.
In previous research [48] the antioxidants showed a positive correlation with the antioxidant activity, whereas their correlation with the temperature was positive in Solanum lycopersicum [49] but negative in Lactuca sativa and Citrullus lanatus [49,50].Indeed, stressing temperature levels enhance the activity of phenylalanine ammonium-lyase (PAL) which is the main enzyme involved in phenolic biosynthesis, catalyzing the production of trans-cinamic acid starting from L-phenylalanine [51], thus promoting cell acclimation [52].On the other hand, the oxidation of o-diphenols to o-diquinons caused by polyphenols oxidases (PPO) or peroxidases (POD), as well as the monophenols hydroxylation are the consequences of physiological disorders due to thermal stress [53].In this respect, the accumulation of soluble phenolics is a mechanism of adaptation to extreme temperatures [49] and the content of these antioxidants is affected by both farming practices and environment [54].In addition, phenolics counteract the degrading enzyme activity of ascorbic acid [55] which exerts essential functions such as antioxidant, photosynthetic enzyme regulating, phytohormonal [56].

Conclusions
The effect of starch-based biodegradable mulch was assessed in research carried out in southern Italy on three crop cycles of Diplotaxis tenuifolia.The biodegradable film did not differ from both standard (LDPE) and photoselective plastic mulch in terms of yield but resulted in the best overall performances with regard to quality and chemical composition.The eco-compatible material also led to higher antioxidants content and activity, but only in comparison to LDPE.Indeed, the biodegradable mulch led to soil temperature increase, but never over the optimal threshold of perennial wall rocket requirement, which occurred in some days under both the standard and photoselective plastic mulch.Further, the biodegradable mulch is an environmentally friendly and cost-effective tool available for sustainable management of crop systems.

Figure 1 .
Figure 1.Trend of air temperature inside the greenhouse in Portici (Naples, southern Italy) as an average of 2016-2017 and 2017-2018.

Figure 1 .
Figure 1.Trend of air temperature inside the greenhouse in Portici (Naples, southern Italy) as an average of 2016-2017 and 2017-2018.

Figure 2 .
Figure 2. Trend of soil temperature of greenhouse perennial wall rocket grown under different mulching types, as an average of 2016-2017 and 2017-2018.

Table 1 .
Mean values of perennial wall rocket precocity, growth indices and yield components as affected by crop cycle and mulch treatment.

Table 2 .
Mean values of SPAD index and color components of perennial wall rocket as affected by crop cycle and mulch treatment.Within each column, n.s.no statistically significant difference, * significant difference at p ≤ 0.05; means followed by different letters are significantly different according to the Duncan test at p ≤ 0.05.

Table 3 .
Mean values of leaf quality indicators of perennial wall rocket as affected by crop cycle and mulch treatment., dry residue.Within each column, n.s.no statistically significant difference, * significant difference at p ≤ 0.05; means followed by different letters are significantly different according to the Duncan test at p ≤ 0.05. DR

Table 4 .
Mean values of mineral composition of perennial wall rocket leaves as affected by crop cycle and mulch treatment.
Within each column, n.s.no statistically significant difference, * significant difference at p ≤ 0.05; means followed by different letters are significantly different according to the Duncan test at p ≤ 0.05.

Table 5 .
Mean values of antioxidant content and activity of perennial wall rocket leaves as affected by crop cycle and mulch treatment.Within each column, * significant difference at p ≤ 0.05; means followed by different letters are significantly different according to the Duncan test at p ≤ 0.05.