Halotolerant Bacterial Diversity Associated with Suaeda fruticosa ( L . ) Forssk . Improved Growth of Maize under Salinity Stress

Halotolerant bacterial strains associated with the rhizosphere and phytoplane of Suaeda fruticosa (L.) Forssk. growing in saline habitats were isolated to mitigate the salinity stress of Zea mays L. 16S rRNA gene sequencing confirmed the presence of strains that belong to Gracilibacillus, Staphylococcus, Virgibacillus, Salinicoccus, Bacillus, Zhihengliuella, Brevibacterium, Oceanobacillus, Exiguobacterium, Pseudomonas, Arthrobacter, and Halomonas genera. Strains were screened for auxin production, 1-aminocyclopropane-1-carboxylate (ACC)-deaminase, and biofilm formation. Bacterial auxin production ranged from 14 to 215 μg mL−1. Moreover, several bacterial isolates were also recorded as positive for ACC-deaminase activity, phosphate solubilization, and biofilm formation. In pot trials, bacterial strains significantly mitigated the salinity stress of Z. mays seedlings. For instance, at 200 and 400 mM NaCl, a significant increase of shoot and root length (up to onefold) was recorded for Staphylococcus jettensis F-11. At 200 mM, Zhihengliuella flava F-9 (45%) and Bacillus megaterium F-58 (42%) exhibited significant improvements for fresh weight. For dry weight, S. jettensis F-11 and S. arlettae F-71 recorded up to a threefold increase at 200 mM over the respective control. The results of this study suggest that natural plant settings of saline habitats are a good source for the isolation of beneficial salt-tolerant bacteria to grow crops under saline conditions.


Introduction
Soil salinity is one of the most severe environmental factors limiting agricultural productivity in arid or semiarid regions around the world.Salinity adversely affects the physical and chemical parameters of the soil as well as crop production to a significant extent [1].Halophytes are the vegetation of saline habitats.Halophytes possess special morphological, anatomical, and physiological characteristics that are well suited to cope with saline habitats.Suaeda fruticosa (L.) Forssk. is a plant species that belongs to the family Amaranthaceae.It is a halophyte that occurs in arid and semiarid saline habitats or salt marches.S. fruticosa has a strong ability to accumulate and sequester NaCl [2].It can be used for soil reclamation to reduce salinity and contamination of heavy metals.Halophytes have a number of adaptations to mitigate salinity stress, including salt discharge from roots, accumulation of organic acids, reduced stomatal conductance, lower water potential, and uptake of inorganic ions [3].Moreover, specific types of amino acids, carbohydrates, and glycine betaine are accumulated as compatible solutes under abiotic stress [4].
High salt content in soil and irrigation water is a major threat to the sustainability of agriculture around the world.The presence of excess salts in the rhizosphere can cause severe injury to the root system followed by their gradual accumulation in other plant tissues.It can cause heavy damage to plant metabolism, which can lead to stunted plant growth and reduced yield [5]. Salinity can disrupt the water uptake and ion equilibrium of plants.It can lead to oxidative damage to plants due to the production of reactive oxygen species (ROS).This includes the production of superoxide anion (O 2 − ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical (OH) etc. Halophytes have the ability to keep the level of these ROS at minimal levels due to the presence of an antioxidant system that consists of enzymes like catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) [6,7].Rhizobacteria associated with the root system of halophytes can enhance the growth, development, and stress tolerance of plants growing in saline soils [8].It has been demonstrated that beneficial microorganisms play a significant role in alleviating salt stress in plants, which results in increased crop yield.Plant-growth-promoting rhizobacteria (PGPR) are a group of microorganisms that colonize the root system of plants and trigger plant growth and development under stress conditions.PGPR can employ several direct or indirect mechanisms to enhance plant growth and productivity.Auxin production, 1-aminocyclopropane-1-carboxylate (ACC)-deaminase, siderophores production, and phosphate solubilization are well documented in the literature [9,10].Indole-3-acetic acid (IAA) represents one of the most extensively studied auxins in plants.It mediates multidimensional developmental processes including apical dominance, tropic responses, cellular differentiation, and pattern formation [11].IAA is also quantitatively the most abundant phytohormones secreted by rhizobacteria as secondary metabolites.Auxin production has been reported for several halotolerant bacterial genera including Bacillus, Enterobacter, Arthrocnemum, Agrobacterium, Ochrobactrum, Pseudomonas, and Pantoea in NaCl-amended medium [8,9].Moreover, soil microorganisms possess the enzyme ACC-deaminase that converts ACC (precursor of ethylene) to α-ketobutyrate and ammonia.When plants are exposed to stress conditions, ethylene is synthesized, which results in retardation of root growth and senescence [12,13].Although ethylene mediates growth responses in plants, it can inhibit plant growth at elevated levels (under stress), leading to physiological changes in plant tissues.Tolerance to biotic or abiotic stresses can be increased by treating plant roots with ACC-deaminase-producing rhizobacteria [14].Halotolerant PGPR have been reported to confer upon plants the ability to mitigate environmental or abiotic stresses [7].For instance, Bacillus aquimaris, Bacillus thuringiensis, Enterobacter cloacae, Enterobacter asburiae, Ochrobactrum anthropi, and Pseudomonas stutzeri significantly increased vegetative and yield parameters under saline conditions [9,10,15].Maize (Zea mays L.) is the third most important cereal crop that is grown under a wide range of climatic conditions.Salt-tolerant PGPR isolated from halophytes have been reported to enhance the vegetative growth parameters of maize under induced salinity [16].
Soil salinity affects approximately 6.3 million hectors of irrigated land in Pakistan and has caused considerable losses to the agriculture sector [17].There is a need to devise environmentally friendly strategies that can ameliorate soil salinity by the application of resident halotolerant bacteria.In the present study, salt-tolerant bacteria associated with halophytic plants have been evaluated to mitigate the salinity stress of maize.In Pakistan, S. fruticosa is distributed in coastal and inland saline habitats [6].
Little is known about the association of halotolerant bacterial communities with this plant.Therefore, the purpose of this study was to evaluate the bacterial diversity of the rhizosphere and phytoplane of S. fruticosa growing in saline habitats of the Photohar Plateau, a region of northeastern Pakistan.Bacterial communities associated with the surfaces of S. fruticosa may have naturally adapted to tolerate high salt concentrations.Therefore, screening of resident bacterial flora may indicate potential candidates for agricultural applications under saline habitats.

Isolation of Bacterial Strains
Suaeda fruticosa (L.) Forssk. is a halophytic plant that grows in the saline habitats of the Photohar Plateau, a region of northeastern Pakistan.Halotolerant bacterial strains were isolated from the rhizosphere and phytoplane of S. fruticosa.For rhizospheric samples, 1 g of soil was dissolved in 99 mL of sterilized distilled water.Then, the serial dilutions of the samples were prepared successively as described in Cappuccino and Sherman [18].For endophytes, leaves were sterilized by soaking in 70% ethanol for 2-3 min and then washed with autoclaved distilled water.One gram of leaves was grounded to paste and transferred to a test tube containing 9 mL of autoclaved distilled water.About 50 µL from soil and leaf suspensions was plated on L-agar supplemented with 0.5 M and 1 M NaCl.Plates were incubated at 30 • C for 24 h.Discrete bacterial colonies were selected and purified by many rounds of streaking.

16S rRNA Gene Sequencing
Bacterial strains were identified by 16S rRNA gene sequencing.
Total genomic DNA from overnight grown bacterial cultures was extracted using the FavorPrep TM Tissue Genomic DNA Extraction Mini Kit (Favorgen Biotech Corporation, Ping-Tung, Taiwan).PCR amplification of 16S rRNA gene sequencing was accomplished by using forward primer 27f (5 -AGAGTTTGATCCTGGCTCAG-3 ) and reverse primer 1522r (5 -AAGGAGGTGATCCA (AG)CCGCA-3 ) [19].PCR amplification was performed in 50 µL of Dream Taq TM Green PCR Master Mix (Fermentas, Waltham, MA, USA) with 0.5 µg of chromosomal DNA template and 0.5 µM of each primer.The reaction mixtures were incubated in a thermocycler Primus 96 (PeQLab, Erlangen, Germany) at 94 • C for 5 min and passed through 30 cycles: Denaturation for 20 s at 94 • C, primer annealing for 20 s at 50 • C, and extension at 72 • C for 2 min.The amplified products were purified by using the FavorPrep TM Gel Purification Mini Kit (Favorgen Biotech Corporation, Ping-Tung, Taiwan).Purified PCR products were sent to First Base Laboratories, Singapore for sequencing.Sequences were aligned with a multiple sequence alignment program (Clustal W) by using Molecular Evolutionary Genetics Analysis (MEGA) 6. software) [20].The phylogenetic relationships among halotolerant bacterial strains were studied by constructing phylogenetic trees by the neighbor-joining method [21].

Halophility Assay
L-broth medium supplemented with 0, 0.5, 1, and 1.5 M NaCl was used to evaluate the salt tolerance of bacterial strains.After inoculation, strains were incubated overnight at 37 • C for 24 h at 130 rpm/min.For all treatments, three replicates were placed for comparison.The optical density of the cultures was recorded at 600 nm by a spectrophotometer (CECIL CE 7200, Cecil Instruments Limited, Cambridge, UK).Proline analysis of bacterial strains grown under salt stress was also accomplished by following the method of Cha-Um and Kirdmanee [22].

Plant-Growth-Promoting Traits
For auxin quantification, strains were inoculated in triplicate in L-broth supplemented with 1 M NaCl and 500 µg mL −1 filter sterilized solution of L-tryptophan.After incubation at 37 • C for 72 h, cells were removed from stationary phase cultures by centrifugation.One milliliter of culture supernatant was mixed with 2 mL of Salkowski reagent [23].Afterwards, samples were incubated in the dark for the development of pink color.Optical density was recorded at 535 nm with a spectrophotometer (CECIL CE 7200).The standard curve was constructed by using different concentrations of authentic auxins to calculate the auxin production by different bacterial strains.The phosphate solubilization potential of bacterial isolates was evaluated by streaking on Pikovskaya's agar plates [24].
The 1-aminocyclopropane-1-carboxylate (ACC)-utilization ability of the strains was evaluated by following the method described in [25].Bacterial strains were inoculated in 5 mL of L-broth in triplicate and incubated overnight at 28 • C on a shaker at 200 rpm/min.Two milliliters of each culture were harvested in a 2-mL Eppendorf tube by centrifugation at 8000× g for 5 min.The cell pellet was washed twice with 1 mL of liquid Dworkin-Foster(DF) medium.Afterwards, the pellet was suspended in 2 mL of DF-ACC medium in a 12-mL culture tube.The tubes were incubated at 28 • C on a shaker at 200 rpm/min for 24 h.A 2-mL sample of DF-ACC medium without inoculation was incubated in parallel.One milliliter of each culture was centrifuged in a 1.5 mL Eppendorf tube as mentioned above.One hundred microliters of each supernatant were diluted to 1 mL with liquid DF medium in a 1.5-mL Eppendorf tube.Sixty microliters of each tenfold-diluted supernatant was used for the 96-well PCR-plate ninhydrin-ACC assay and mixed with 120 µL of ninhydrin reagent.The PCR plate was then heated on a boiling water bath for 30 min.The DF medium was used as a blank.Each diluted supernatant was run in triplicate.After transfer of 100 µL of the remaining reaction solution, the absorbance of the samples was measured at 570 nm with a microplate spectrophotometer (Epoch, BioTek, Winooski, VT, USA).

Biofilm Formation
The biofilm-forming ability of bacterial strains was evaluated by following the method of [26].Bacterial strains were inoculated in triplicate in Tryptic Soy Broth (TSB) supplemented with 0, 200, and 400 mM NaCl and incubated at 37 • C for 24 h.Following incubation, 20 µL of the cultures was transferred to the wells of a 96-well flat bottom microtiter plate that contained 180 µL of TSB supplemented with the abovementioned NaCl concentrations.Negative controls contained only 200 µL of the TSB medium and the assay was performed in triplicate.To promote biofilm formation, the plates were incubated on a shaker at 37 • C for 72 h.After incubation, the well contents were discarded and washed thrice with 250 µL of sterile distilled water to remove any nonadherent and weakly adherent cells.Plates were air dried for 30 min.The biofilm formed in the wells was fixed with 250 µL/well 98% methanol for 15 min.After air drying, the fixed bacterial cells were stained with 200 µL of 0.1% v/v crystal violet solution for 5 min.Excessive stain was removed by placing the plate under low-running tap water and the plate was air dried.Resolubilization of crystal violet with the adherent cells was done by adding 200 µL/well of 33% v/v glacial acetic acid.The optical density was measured at 570 nm using a microplate spectrophotometer (Epoch, BioTek, Winooski, VT, USA).

In Vitro Plant Bioassay
Seeds of maize (Zea mays L. Var.Sahiwal-2002) were obtained from Punjab Seed Corporation (Lahore, Pakistan).Seeds were surface sterilized by treatment with 0.1% HgCl 2 for 2-3 min followed by repeated washing with sterilized distilled water.Rooting assay was performed with single or mixed bacterial cultures.Single bacterial cultures included F-9, F-11, F-12, F-35, F-37, F-58, F-71, F-72, F-81, F-83, F-84, and F-87.Mixed culture combinations C-1 (F-9, F-11, F-12, F-87), C-2 (F-35, F-71, F-81, F-83), and C-3 (F-37, F-58, F-72, F-84) were also included for seed inoculation.For inoculum preparation, strains were cultivated in L-broth for 24 h.Then, cultures were harvested by centrifugation and the cell pellet was washed with autoclaved distilled water.The optical densities of the cultures were adjusted to a final concentration of 10 7 colony forming units (CFU)/mL in sterilized distilled water.Sterilized seeds were soaked in a bacterial suspension for 30 min and placed in Petri plates containing two sterilized filter papers.Water-treated seeds were used for comparison.For each strain and combination, five seeds were inoculated in duplicate.Petri plates were incubated in an Environmental Test Chamber (MRL-350H; Sanyo, Osaka, Japan) at 25 • C.After 7 days, shoot length, root length, number of roots, and fresh and dry weights of seedlings were recorded.

Pot Trials with Salt Stress
Pot experiments were conducted to evaluate the growth-promoting effect of bacterial strains on maize under different salt-stress conditions.Seeds were surface sterilized as mentioned earlier and placed on moistened autoclaved double-filter paper in Petri plates.Healthy germinated seeds were selected for bacterization with single and mixed cultures of bacterial strains as mentioned above.Germinated seeds were sown in pots containing 100 g of autoclaved soil.Five seeds were sown in each pot and experiments were carried out in triplicate.Three salt-stress conditions, i.e., 0, 200, and 400 mM, were applied up to the field capacity of soil.Salt stress was applied twice-at the time of sowing and to 7-day-old seedlings.Water-treated seeds were used as a control.Pots were kept under conditions of 12 h of photoperiod and a temperature of 25 • C in the Environmental Test Chamber (MRL-350H; Sanyo, Osaka, Japan).After two weeks, plant-growth parameters such as shoot length, root length, number of roots, and fresh weight of seedlings were recorded.For dry weight, plants were incubated in an oven at 80 • C for 24 h.

Biochemical Analysis of Plants
The proline content of plants grown under 0, 200, and 400 mM NaCl was measured as described by Cha-Um and Kirdmanee [22].Similarly, the method of Racusen and Foote [27] was used for the quantitative estimation of peroxidases from fresh leaves.Acid phosphatase was extracted from plant leaves following the method of Iqbal and Rafique [28].

Statistical Analysis
Data for bacterial auxin production and plant-growth parameters were subjected to statistical analysis by using SPSS 20 software (IBM Corporation, New York, NY, USA).The data were also subjected to analysis of variance (ANOVA).Finally, mean values were separated by using Duncan's multiple range test (p ≤ 0.05).Correlation analysis between bacterial growth and NaCl concentrations was also performed (p = 0.05).

Strains Identification
One hundred salt-tolerant bacterial strains were isolated from the rhizosphere and phytoplane of Suaeda fruticosa (L.) Forssk.fruticosa growing in saline habitats.16S rRNA gene sequences were compared with already-deposited sequences in GenBank.The comparison indicated around 99% similarity with respective identified species.Analysis showed that the strains belong to the genera Gracilibacillus, Staphylococcus, Virgibacillus, Salinicoccus, Bacillus, Zhihengliuella, Brevibacterium, Oceanobacillus, Exiguobacterium, Pseudomonas, Arthrobacter, and Halomonas.Sequences were submitted to GenBank under accession numbers KT027652-KT027742.Rhizospheric soil samples recorded the presence of 45 salt-tolerant rhizobacteria and were represented by 9 bacterial genera (Table 1).Similarly, the phytoplane recorded the presence of 46 endophytic bacterial strains represented by 8 bacterial genera (Table 2).Figure 1 shows the phylogenetic relationships among different salt-tolerant bacteria isolated from the rhizosphere and phytoplane of S. fruticosa.Analysis showed that the majority of the Gram-positive strains (firmicutes) clustered in one major group.This included strains from the genera Bacillus and Staphylococcus.A few Gram-positive strains were also represented by the genera Oceanobacillus, Salinococcus, Gracilibacillus, Brevibacterium, and Exiguobacterium.In addition to that, Gram-positive Arthrobacter and Zhihengliuella clustered in a separate group.Gram-negative strains were represented by the genera Pseudomonas and Halomonas and occupied a separate cluster in the phylogenetic tree.

Halophility Assay
Bacterial isolates showed variable growth responses at different NaCl concentrations (Figure 2).The majority of the isolates that included S. jettensis F-11, S. arlettae F-12, S. caprae F-37, S. arlettae F-71, S. jettensis F-72, and B. safensis F-83 showed good growth up to 1.5 M NaCl.A few strains recorded sensitivity against increasing levels of NaCl.For example, Z. flava F-9, B. megaterium F-58, and B. pumilus F-84 recorded poor growth as compared to other halotolerant bacterial strains.Proline is produced as an osmoprotectant under osmotic stress induced at high salinity.It accumulates as a compatible solute in living cells to counter the effects of NaCl toxicity.Bacterial proline content did not show a significant difference with the increasing salt content from 0 to 1.5 M NaCl.The highest concentration of proline was observed with S. jettensis F-11 (228 µg g −1 ) and S. jettensis F-72 (216 µg g −1 ) at 1.5 M NaCl (Figure 3).

Plant-Growth-Promoting Traits
One hundred bacterial strains were screened for in vitro auxin production by colorimetric analysis.Screening indicated significant auxin production by 30 bacterial strains (Table 3).In the absence of L-tryptophan, bacterial auxin production ranged from 5.70 to 87.90 µg mL −1 .Bacterial strains produced significant levels of auxin in culture supernatant in the presence of 500 µg mL −1 L-tryptophan as compared to the nonamended control.For instance, S. petrasii F-2, V. salarius F-3, Z. flava F-9, S. jettensis F-11, S. arlettae F-12, and S. arlettae F-71 recorded 10-, 2-, 5-, 3-, 3-, and 2-fold increases in auxin production over the control.Overall, auxin production ranged from 14 to 215 µg mL −1 in L-tryptophan-amended medium.On the other hand, 14 isolates were recorded as positive for phosphate solubilization.S. jettensis F-11, S. arlettae F-12, and S. jettensis F-72 were the most efficient as compared to other strains.
Bacterial isolates showed variable potential for ACC-deaminase activity.Highly significant activity was observed for S. caprae F-37, S. arlettae F-71, B. subtilis F-14, E. mexicanum F-35, and O. kapialis F-44.For biofilm formation, S. caprae F-37 and B. pumilus F-84 recorded good production at 200 mM NaCl.However, at higher NaCl levels, poor biofilm formation was recorded for the majority of the strains (Figure 4).

Plant-Growth-Promoting Traits
One hundred bacterial strains were screened for in vitro auxin production by colorimetric analysis.Screening indicated significant auxin production by 30 bacterial strains (Table 3).In the absence of L-tryptophan, bacterial auxin production ranged from 5.70 to 87.90 µg mL −1 .Bacterial strains produced significant levels of auxin in culture supernatant in the presence of 500 µg mL −1 L-tryptophan as compared to the nonamended control.For instance, S. petrasii F-2, V. salarius F-3, Z. flava F-9, S. jettensis F-11, S. arlettae F-12, and S. arlettae F-71 recorded 10-, 2-, 5-, 3-, 3-, and 2-fold increases in auxin production over the control.Overall, auxin production ranged from 14 to 215 µg mL −1 in L-tryptophan-amended medium.On the other hand, 14 isolates were recorded as positive for phosphate solubilization.S. jettensis F-11, S. arlettae F-12, and S. jettensis F-72 were the most efficient as compared to other strains.
Bacterial isolates showed variable potential for ACC-deaminase activity.Highly significant activity was observed for S. caprae F-37, S. arlettae F-71, B. subtilis F-14, E. mexicanum F-35, and O. kapialis F-44.For biofilm formation, S. caprae F-37 and B. pumilus F-84 recorded good production at 200 mM NaCl.However, at higher NaCl levels, poor biofilm formation was recorded for the majority of the strains (Figure 4).

Plant-Growth-Promoting Traits
One hundred bacterial strains were screened for in vitro auxin production by colorimetric analysis.Screening indicated significant auxin production by 30 bacterial strains (Table 3).In the absence of L-tryptophan, bacterial auxin production ranged from 5.70 to 87.90 µg mL −1 .Bacterial strains produced significant levels of auxin in culture supernatant in the presence of 500 µg mL −1 L-tryptophan as compared to the nonamended control.For instance, S. petrasii F-2, V. salarius F-3, Z. flava F-9, S. jettensis F-11, S. arlettae F-12, and S. arlettae F-71 recorded 10-, 2-, 5-, 3-, 3-, and 2-fold increases in auxin production over the control.Overall, auxin production ranged from 14 to 215 µg mL −1 in L-tryptophan-amended medium.On the other hand, 14 isolates were recorded as positive for phosphate solubilization.S. jettensis F-11, S. arlettae F-12, and S. jettensis F-72 were the most efficient as compared to other strains.
Bacterial isolates showed variable potential for ACC-deaminase activity.Highly significant activity was observed for S. caprae F-37, S. arlettae F-71, B. subtilis F-14, E. mexicanum F-35, and O. kapialis F-44.For biofilm formation, S. caprae F-37 and B. pumilus F-84 recorded good production at 200 mM NaCl.However, at higher NaCl levels, poor biofilm formation was recorded for the majority of the strains (Figure 4).F-11 and S. arlettae F-71 recorded two-to threefold increases over the 200 mM control.At 400 mM salt stress, the mixed culture C-1 gave good results for dry weight.

Antioxidant Analysis
Bacterial inoculations recorded high proline accumulation in plants.At 200 mM NaCl, S. arlettae F-12, B. safensis F-83, and C-1 (mixed culture) recorded the highest accumulation of proline.Similarly, at 400 mM salt stress, S. arlettae F-12 and S. arlettae F-71 resulted in considerable increases in proline concentrations over water-treated control plants (Figure 5a).Regarding peroxidases, plants produced a very low content in water-treated seeds.However, with increasing salinity (200-400 mM), considerable enhancements in peroxidase activity was observed (Figure 5b).Acid phosphatase production significantly increased at 200 mM NaCl with Z. flava F-9, S. arlettae F-12, and S. jettensis F-72.At 400 mM salt stress, Z. flava F-9, S. jettensis F-72, and B. safensis F-83 recorded up to twofold increases for acid phosphatase content over the control (Figure 5c).

Antioxidant Analysis
Bacterial inoculations recorded high proline accumulation in plants.At 200 mM NaCl, S. arlettae F-12, B. safensis F-83, and C-1 (mixed culture) recorded the highest accumulation of proline.Similarly, at 400 mM salt stress, S. arlettae F-12 and S. arlettae F-71 resulted in considerable increases in proline concentrations over water-treated control plants (Figure 5a).Regarding peroxidases, plants produced a very low content in water-treated seeds.However, with increasing salinity (200-400 mM), considerable enhancements in peroxidase activity was observed (Figure 5b).Acid phosphatase production significantly increased at 200 mM NaCl with Z. flava F-9, S. arlettae F-12, and S. jettensis F-72.At 400 mM salt stress, Z. flava F-9, S. jettensis F-72, and B. safensis F-83 recorded up to twofold increases for acid phosphatase content over the control (Figure 5c).

Discussion
Soil salinity severely affects crop productivity and resident soil microbial communities.In Pakistan, soil salinity results in approximately 26% losses for the agroeconomy because it affects the germination, growth, and respiration of seeds [17].The present study was carried out to examine the bacterial diversity of S. fruticosa growing in saline habitats of northeastern Pakistan.Extreme habitats have been reported to be colonized by a variety of microorganisms that are well adapted to these environments [29][30][31].Natural plant settings growing in these localities may harbor beneficial microbes for agricultural applications under saline conditions [9]. S. fruticosa is a halophytic plant that mainly colonizes saline habitats.Therefore, we targeted the isolation of plant-growth-promoting rhizobacteria (PGPR) associated with the surfaces and rhizosphere of this plant.Analysis of 16S rRNA gene sequences confirmed the presence of several strains of halotolerant bacteria.Since little is known about the bacterial diversity associated with S. fruticosa, we therefore reported in detail the diversity and agricultural significance of bacterial communities colonizing the surfaces of this halophytic plant.
Bacterial strains showed good potential for salt tolerance and production of proline up to 1.5 M NaCl stress.Especially, E. mexicanum F-35 recorded a significant positive correlation (r = 0.967; p = 0.05) with increasing NaCl concentration in culture media.Inoculation of Z. mays by halotolerant rhizobacteria also influenced the production of antioxidant enzymes.For instance, plants grown under salinity stress exhibited the accumulation of proline as a compatible solute to retain water that otherwise might be lost due the surrounding hypertonic environment.Similarly, bacterial inoculations also influenced the peroxidase and acid phosphatase contents of plants.Peroxidases and acid phosphatases are considered very important enzymes to mitigate salinity-induced stresses.Exposure of plants to environmental stresses can lead to the production of reactive oxygen species (ROS) that can damage plant macromolecules.In addition to plants, microbes can augment the supply of enzymatic and nonenzymatic metabolites to detoxify the impact of ROS [32,33].Previously, bioassays for the antioxidant system have also been reported with different plants and microorganisms [34,35].
In the present study, halotolerant rhizobacteria also showed good potential to produce a variety of plant-growth-promoting attributes.For example, strains recorded up to a 10-fold increase in auxin content in the presence of L-tryptophan over the unamended control.Under in vitro conditions, strains of genus Staphylococcus (F-12, F-71, F-72) were the most promising to enhance the rooting or shooting of inoculated maize plants.In pot trials, strains belonging to Bacillus, Staphylococcus, Zhihengliuella, and Halomonas genera were very effective at enhancing plant growth in salt-amended soils.However, strains of S. arlettae (F-12, F-71) recorded consistent results under both sets of experiments.Auxin-producing PGPR have been reported to improve plant growth under different sets of conditions.Charcoal-based formulations of rhizobacteria with auxin-production ability have been shown to enhance the vegetative and yield parameters of wheat [36].Previously, we also reported the growth and yield improvement of plants grown under different salinity and water stresses by auxin-producing rhizobacteria [9,33].Defez et al. [37] demonstrated the role of bacterial auxin in the upregulation of nitrogenase activity of inoculated rice plants.Inoculation of maize seeds with auxin-producing B. megaterium and Azotobacter chroococcum significantly enhanced the rooting and shooting compared to control seedlings [38].Bacterial auxin has also been shown to alleviate the salt stress of plants grown under saline conditions.For instance, halotolerant bacteria from saline habitats stimulated plant growth by mitigating osmotic stress [9,39].Similarly, salt-tolerant rhizobacteria exhibiting multiple plant-growth-promoting traits stimulated the growth of wheat in salt-affected soils [40].In another study, strains of Bacillus facilitated the growth of maize under water-stress conditions [41].
High salinity levels in soil or in irrigation water are a major threat to the sustainability of agricultural production.The accumulation of salts in the vicinity of a plant's rhizosphere can cause severe injury to the root system.High concentrations of salts can result in the production of a gaseous hormone ethylene after seed germination.Elevated levels of ethylene can suppress the root system, which can result in compromised plant growth and development.Salt-tolerant rhizobacteria have the ability to produce the enzyme ACC-deaminase, which can degrade the substrate (ACC) of ethylene into α-ketobutyrate and ammonia [13,32].Hence, the ACC-deaminase activity of rhizobacteria mitigates deleterious levels of stress-induced ethylene.In the present study, highly significant ACC-deaminase activity was exhibited by S. caprae F-37, S. arlettae F-71, and B. subtilis F-14.PGPR with ACC-deaminase activity can protect inoculated plants from the deleterious effects of abiotic stressors.Orhan et al. [42] reported the salt-stress alleviation of wheat by halotolerant and halophilic PGPR that exhibited ACC-deaminase activity.Halotolerant Klebsiella with ACC-deaminase activity has also been shown to promote the vegetative growth parameters and chlorophyll content of plants under salt stress [43].Similarly, Tank and Saraf [44] reported the positive effects of ACC-deaminase-containing bacteria on plant growth against salinity stress.
Biofilm formation is another important trait of PGPR that can help plants tolerate abiotic stress.It is a complex association of bacterial cells attached to the plant's root system and plays a very critical role in retaining moisture and protecting against different biotic or abiotic stresses.In this study, S. caprae F-37 and B. pumilus F-84 demonstrated good production of biofilm at 200 mM NaCl.The biofilm-forming ability of halotolerant rhizobacteria has been shown to enhance plant biomass under saline conditions [45].Inoculation of barley plants with biofilm-forming B. amyloliquefaciens ameliorated salt stress and also stimulated different plant-growth parameters [46].

Conclusions
Finally, it can be concluded that S. fruticosa growing in saline areas harbor a range of beneficial plant-growth-promoting rhizobacteria.Bacterial strains showed a good ability to grow in up to 1.5 M NaCl.Halotolerant bacterial strains showed good auxin-production potential, ACC-deaminase activity, and biofilm formation.In pot trials, strains recorded good results for the amelioration of salt stress of maize plants.Especially, S. jettensis F-11, S. arlettae F-12, B. marisflavi F-87, Z. flava F-9, and H. nanhaiensis F-81 were shown to exhibit multiple plant-growth-promoting traits.In pot trials, strains were also very effective at mitigating the salinity stress of maize plants.For instance, S. jettensis F-11, F-12, S. arlettae F-71, B. marisflavi F-87, H. nanhaiensis F-81, Z. flava F-9, and E. mexicanum F-35 enhanced plant-growth parameters under salt stress.The data presented in this work are very encouraging for the use halotolerant rhizobacteria to enhance plant growth under salinity stress.The results of this study also indicated that the natural plant settings of a saline habitat are a good source for isolating beneficial PGPR to grow crops under saline conditions.

Figure 1 . 17 Figure 2 .
Figure 1.Phylogenetic tree showing the relationships among different halotolerant bacterial strains isolated from rhizosphere and phytoplane of S. fruticosa.(L.) Forssk.Scale bar represents mutations per nucleotide position.

Figure 3 .
Figure 3.Effect of NaCl concentrations on proline content of halotolerant bacteria.Bars represent mean ± SE of three replicates.Treatments followed by * indicate significant difference over control using Duncan's multiple range test (p ≤ 0.05).

Figure 2 . 17 Figure 2 .
Figure 2. Halophility assay of salt-tolerant rhizobacteria in the presence of different concentrations of NaCl.Bar represents mean ± SE of three replicates.

Figure 3 .
Figure 3.Effect of NaCl concentrations on proline content of halotolerant bacteria.Bars represent mean ± SE of three replicates.Treatments followed by * indicate significant difference over control using Duncan's multiple range test (p ≤ 0.05).

Figure 3 .
Figure 3.Effect of NaCl concentrations on proline content of halotolerant bacteria.Bars represent mean ± SE of three replicates.Treatments followed by * indicate significant difference over control using Duncan's multiple range test (p ≤ 0.05).

Figure 4 .
Figure 4. Effect of different NaCl concentrations on biofilm-forming ability of bacterial strains.Bars represent mean ± SE of three replicates.Treatments followed by * indicate significant difference over control using Duncan's multiple range test (p ≤ 0.05).

Figure 4 .
Figure 4. Effect of different NaCl concentrations on biofilm-forming ability of bacterial strains.Bars represent mean ± SE of three replicates.Treatments followed by * indicate significant difference over control using Duncan's multiple range test (p ≤ 0.05).
plants.Different letters within same column indicates significant difference between treatments using Duncan's multiple range rest (p ≤ 0.05); * Mixed culture combinations.

Figure 5 .
Figure 5.Effect of single and mixed bacterial inoculations and different salinity levels on the activities of antioxidant metabolites or enzymes: (a) Proline; (b) Peroxidase; (c) Acid phosphatase.Bars represent mean ± SE of three replicates.Treatments followed by * indicate significant difference over respective control using Duncan's multiple range test (p ≤ 0.05).

9 F- 11 F 9 F- 11 FFigure 5 .
Figure 5.Effect of single and mixed bacterial inoculations and different salinity levels on the activities of antioxidant metabolites or enzymes: (a) Proline; (b) Peroxidase; (c) Acid phosphatase.Bars represent mean ± SE of three replicates.Treatments followed by * indicate significant difference over respective control using Duncan's multiple range test (p ≤ 0.05).

Table 2 .
16S rRNA gene sequencing of halotolerant bacterial strains isolated from the phytoplane of S. fruticosa.

Table 3 .
L-tryptophan-dependent bacterial auxin production in the presence of 1 M NaCl.
Mean of three replicates.Different letters followed by numerical values within same column indicate significant difference between treatments using Duncan's multiple range test (p ≤ 0.05).

Table 3 .
L-tryptophan-dependent bacterial auxin production in the presence of 1 M NaCl.

Table 5 .
Effect of halotolerant bacterial strains on the growth of Z. mays in salt amended soils.