Jerusalem Artichoke as a Strategic Crop for Solving Food Problems

: Jerusalem artichoke is a staple for the raw material base of healthy food, beverages, feed, medicine, and fuel, in addition to being the source of its herbage and tubers. The choice of conditions for cultivating a variety depends on the purpose or direction for the crop and the products obtained from it. This research involved laboratory and on-farm studies of 16 Jerusalem artichoke varieties of different maturity groups cultivated on sod-podzolic sandy loam soils to determine their suitability for further use in feed production. The list of varieties in question is presented in this work. The gross yield of tubers obtained from varieties with early leaf wilting was 33.1–51.1 t/ha, whereas that from varieties with late leaf wilting was 14.4–43.9 t/ha. The total yield of Jerusalem artichoke raw biomass was 36.8–98.1 t/ha. Moreover, early varieties had a dominant mass of tubers in the total feed value structure (68%), whereas herbage mass (59%) prevailed in the total feed value structure of varieties with late leaf wilting. The highest total solids in tubers (25.0–26.4%) were found in varieties Korenevskiy, Novost VIRa, and Blank Brekos; whereas high inulin content was found in varieties Dieticheskiy, Kaluzhskiy, Korenevskiy, Nakhodka, Novost VIRa, and Blank Brekos. The aggregate feed value amounted to 37.0–103.4 thousand MJ/ha of metabolized energy; this exceeded energy production costs on properly selected varieties by two- to three-fold. The data obtained can be useful for growers in the introduction and evaluation of Jerusalem artichoke varieties to be cultivated for further usage and processing, including fodder purposes.


Introduction
Jerusalem artichoke has recently received increased attention due to its remarkable properties as a strategic crop for multiple uses in solutions to food and feed problems. The large amounts of inulin, fructose, and pectin enhance the value chain of Jerusalem artichoke (Helianthus tuberosus L.). The content of inulin, a polysaccharide found in Jerusalem artichoke tubers, is 10-25% of the tuber weight. Moreover, Jerusalem artichoke herbage is characterized by a rich carbohydrate complex (fructose, glucose, sucrose, fructosides, etc.), enabling 83.2 L of alcohol per ton to be obtained. The crop solids contain up to 17% protein with a balanced amino acid composition. Several studies have shown that the introduction of Jerusalem artichoke inulin and bioethanol waste products into feed composition can increase the economic efficiency of animal husbandry and ensure the environmental safety of animal products [1][2][3]. In this context, Jerusalem artichoke has made its way into European and American diets, and production systems for drugs, food, and alcohol. At present, the global growing area under Jerusalem artichoke is about 2.5 million hectares; in France, for example, it is almost equal to the area under potatoes, i.e., over 250,000 hectares, with a total yield of 7.5 million tons. Canada produces 13 million tons of Jerusalem artichoke. In the USA, it is known as topinambour (earth apple) and We believe that crop producers will find our results effective in the introduction and evaluation of Jerusalem artichoke varieties to be cultivated for further usage and processing, including for fodder purposes. Producers may benefit from our practices and discoveries as they grow and experiment with Jerusalem artichoke on their own farm units.

Soils of Test Plots
The Moscow Region is a constituent entity of the Russian Federation. It belongs to the Central Federal District. Despite a wide range of soil compositions and fertility levels, and precipitation amounts and degrees of uniformity, the middle part of the territory of Russia (including the Central, Central Black Earth, and Middle Volga regions, Western Siberia, and the Urals mountainous region) is commonly characterized by a relatively moderate background of infectious load, and can be considered to be a suitable region for establishing private production of high-quality Jerusalem artichoke for seeds and forage [19,20].
The research results were obtained based on laboratory-field experiments established on soils typical of the Non-Chernozem zone of the Russian Federation in terms of their agriphysical and agrichemical properties.

Agrometeorological Conditions for Research Conducting
We carried out the research in the period of 2014-2016 at the test plot of the "Korenevo" Experimental Center; on-the-farm tests were conducted in 2016 at LLC "Kaluga Agro" of the Dzerzheyunskiy district of the Kaluga region.
The meteorological conditions during the years of the research are presented in Figure 1. The hydrothermal coefficient according to Selyaninov (HTC), the value of which is closely related to the yield level, reflects the broadest characteristic of weather conditions. The coefficient is determined by dividing the precipitation amount of the calculated period by the number of effective (10 • C) temperatures, reduced by 10 times over the same period. A decrease in yield was observed at HTC ≤ 0.8 during the period of tuberization. When HTC was more than 3.0, yield decreases resulted from moisture excess [32].
The following operations were performed: (a) 2-track disk harrowing of green m nure crops (vetch + oats) in the first ten-day period of August of the previous year; ( under winter plowing (the first ten-day period of October) with a spinner-type plo without a plough-point to a depth of 18-20 cm; (c) spring continuous cultivation wi harrowing (second and third ten-day periods of April) using a solid cultivator with crust buster attached; and (d) ridge-forming tillage using a row cultivator with fertiliz dispensers. Seed preparation involved calibration and selection of healthy tubers. See tubers having a transverse diameter of 25-40 mm were planted to a depth of 8-10 cm o the test plot using a potato planter with manual tuber feeding.
Field experiments were carried out during the 2014, 2015, and 2016 growing seaso at the "Korenevo" Experimental Center, and LLC "Kaluga Agro" of the Dzerzheyu d trict of the Kaluga region. Plots of the different varieties were arranged in a randomize block design with four replications. The plant density was 25,000 units/ha (75 × 53 cm The plot area was 20 m 2 . Jerusalem artichoke planting was undertaken on 27-30 Ap each research year. Two fertilization bands of azofoska (N60P60K60) were applied locally in May usin a row-crop cultivator with fertilizer dispensers. The bands were about 4 cm wide. Th were placed 4-5 cm on each side from the ridge center and 3-4 cm below the tuber row Crop tending included two inter-row cultivations with hilling using a row cultivator  The following operations were performed: (a) 2-track disk harrowing of green manure crops (vetch + oats) in the first ten-day period of August of the previous year; (b) under winter plowing (the first ten-day period of October) with a spinner-type plow without a plough-point to a depth of 18-20 cm; (c) spring continuous cultivation with harrowing (second and third ten-day periods of April) using a solid cultivator with a crust buster attached; and (d) ridge-forming tillage using a row cultivator with fertilizer dispensers. Seed preparation involved calibration and selection of healthy tubers. Seed tubers having a transverse diameter of 25-40 mm were planted to a depth of 8-10 cm on the test plot using a potato planter with manual tuber feeding.
Field experiments were carried out during the 2014, 2015, and 2016 growing seasons at the "Korenevo" Experimental Center, and LLC "Kaluga Agro" of the Dzerzheyu district of the Kaluga region. Plots of the different varieties were arranged in a randomized block design with four replications. The plant density was 25,000 units/ha (75 × 53 cm). The plot area was 20 m 2 . Jerusalem artichoke planting was undertaken on 27-30 April each research year. Two fertilization bands of azofoska (N60P60K60) were applied locally in May using a row-crop cultivator with fertilizer dispensers. The bands were about 4 cm wide. They were placed 4-5 cm on each side from the ridge center and 3-4 cm below the tuber row. Crop tending included two inter-row cultivations with hilling using a row cultivator in May-June; the first was performed at the pre-emergence stage and the second was conducted at the post-emergence stage. Weed control was performed using a spray hose with herbicides (the first treatment with Zenkor at a rate of 600 g/ha + Titus 30 g/ha + Trend 200 mL/ha), in May.

Methodology of Field Experiments, Observations, Analyzes and Reports
Trial establishment, records and observations were carried out in accordance with the requirements of the field experiment methodology [33] and the program and methodology for evaluating Jerusalem artichoke varieties in test nurseries [34].
Agrochemical soil parameters were analyzed during field reconnaissance in accordance with the methodology for soil sample selection in elementary field areas to apply fertilizers differentially. Agrochemical characteristics of the soil before fertilization were determined as follows: humus-according to Tyurin ( The yield report was compiled manually by weighing herbage (tubers) from 4-8 plants: the first was at the flowering phase of varieties with early leaf wilting; the second was at the end of October.
The following factors were determined in the cropping samples at the flowering phase of early varieties according to all variants of the experiment:

1.
Indicators of herbage forage qualities: maximum plant height, cm; leaf area, m 2 /plant; herbage amount, t, in terms of 1 hectare; nutritional value of the feed in megajoules of metabolizable energy.

2.
Productivity of tubers, t/ha and indicators of tuber quality [35]: crop marketability, tuber shape, skin color, skin surface, dry matter content using the thermostat-weight method; nitrate content using the ionometric method (with a nitratomar-tester); the content of soluble and insoluble substances using the hot water diffusion method; and carbohydrate content, %, using the method of liquid chromatography.

4.
Bioenergetic efficiency of technological method combinations according to the JSC Information and publishing center PATENT methodology [36] and the bioenergy assessment method [37].

5.
Analysis of variance of the data obtained according to Dospekhov B.A. [33].
The research reliability was confirmed by the accepted methodology for conducting a 3-year field study and statistical processing results, obtained by methods of variance, correlation, and regression analyses.

Record of Jerusalem Artichoke Tubers' Yield and Quality
For each variety, tuber yield was registered in two periods (Table 2): the flowering phase of varieties with an early period of top wilting (early September) and the phase of budding-flowering of varieties with a late period of top wilting (the third ten-day period of October). Being harvested at the end of August or at the beginning of September, early varieties produced, on average, 69% of marketable tubers, whereas late varieties produced, on average, only 48% of marketable tubers. However, harvesting of early varieties in the third ten-day period of October resulted in 82% of marketable tubers, and late varieties produced 65% of marketable tubers ( Figure 2).

The Value of Jerusalem Artichoke as a Forage Crop
Expressed in megajoules of metabolizable energy per 1 ha, the total productivity of various Jerusalem artichoke varieties grown on sod-podzolic sandy loam soils under the conditions of the "Korenevo" Experimental Center, Lyuberetskiy District, Moscow Region, is shown Table 3 and Figure 3.

The Value of Jerusalem Artichoke as a Forage Crop
Expressed in megajoules of metabolizable energy per 1 ha, the total productivity of various Jerusalem artichoke varieties grown on sod-podzolic sandy loam soils under the conditions of the "Korenevo" Experimental Center, Lyuberetskiy District, Moscow Region, is shown Table 3 and Figure 3.

Analyses of Tuber Properties of Jerusalem Artichoke Varietal Resources
Jerusalem artichoke tubers were assessed according to morphological characteristics. Tubers' shape, skin color, and surface were analyzed, and the average length ( Figure  4) and outermost transverse diameter of large tubers were estimated ( Figure 5).

Analyses of Tuber Properties of Jerusalem Artichoke Varietal Resources
Jerusalem artichoke tubers were assessed according to morphological characteristics. Tubers' shape, skin color, and surface were analyzed, and the average length ( Figure 4) and outermost transverse diameter of large tubers were estimated ( Figure 5).   The content of dry matter, total sugars, and inulin in tubers of the studied Jerusalem   The content of dry matter, total sugars, and inulin in tubers of the studied Jerusalem artichoke varieties was analyzed ( Figure 6). The content of dry matter, total sugars, and inulin in tubers of the studied Jerusalem artichoke varieties was analyzed ( Figure 6).
x FOR PEER REVIEW 10 of 18

Economic and Energy Efficiency of Jerusalem Artichoke Cultivation
The energy costs and feed values were calculated in megajoules of metabolized energy (Figure 8.

Economic and Energy Efficiency of Jerusalem Artichoke Cultivation
The energy costs and feed values were calculated in megajoules of metabolized energy (Figure 8.

Economic and Energy Efficiency of Jerusalem Artichoke Cultivation
The energy costs and feed values were calculated in megajoules of metabolized energy (Figure 8).

Tubers' Yield Report of Jerusalem Artichoke Varieties
Being harvested in early September (Table 2, Figure 2), the following varieties produced tubers with a fraction of more than 30 mm in the transverse diameter, thus meeting the requirement of more than 25.0 t/ha: Dieticheskiy (40.1 t/ha), Korenevskiy (31, 7 t/ha), and Blank Brekos (27.1 t/ha). The varieties Nakhodka (21.2 t/ha), Podmoskovniy (24.4 t/ha), and Skorospelka (16.7 t/ha) produced a yield that was slightly lower than 25 t/ha. Jerusalem artichoke is a unique crop, all parts of which can be used for benefit (large and small tubers, stems, leaves, flowers, stolons, and roots) [38,39]. Therefore, it is advisable to not only take into account the yield of tubers having a coarse fraction of more than 30 mm in the transverse diameter, but also that of small tubers (less than 30 mm). In addition, Jerusalem artichoke total (gross) yield in accordance with the requirement (more than 25.0 t/ha) was obtained from the following varieties: Dieticheskiy (43.5 t/ha), Nadezhda (40.8 t/ha), Korenevskiy (35.9), Blank Brekos (30.8 t/ha), and Podmoskovniy (26.5 t/ha).
To process tubers in August-September, it is preferable to use early Jerusalem artichoke varieties that, during this period, do not have time to grow and ratoon too much,

Tubers' Yield Report of Jerusalem Artichoke Varieties
Being harvested in early September (Table 2, Figure 2), the following varieties produced tubers with a fraction of more than 30 mm in the transverse diameter, thus meeting the requirement of more than 25.0 t/ha: Dieticheskiy (40.1 t/ha), Korenevskiy (31.7 t/ha), and Blank Brekos (27.1 t/ha). The varieties Nakhodka (21.2 t/ha), Podmoskovniy (24.4 t/ha), and Skorospelka (16.7 t/ha) produced a yield that was slightly lower than 25 t/ha. Jerusalem artichoke is a unique crop, all parts of which can be used for benefit (large and small tubers, stems, leaves, flowers, stolons, and roots) [38,39]. Therefore, it is advisable to not only take into account the yield of tubers having a coarse fraction of more than 30 mm in the transverse diameter, but also that of small tubers (less than 30 mm). In addition, Jerusalem artichoke total (gross) yield in accordance with the requirement (more than 25.0 t/ha) was obtained from the following varieties: Dieticheskiy (43.5 t/ha), Nadezhda (40.8 t/ha), Korenevskiy (35.9), Blank Brekos (30.8 t/ha), and Podmoskovniy (26.5 t/ha).
When the crop was harvested in the third ten-day period of October, the yield of tubers with a fraction of more than 30 mm in the transverse diameter, in accordance with the requirement (more than 25.0 t/ha), was obtained from the following varieties: Nakhodka Furthermore, all varieties of early leaf wilting met the requirement of producing a total (gross) yield of more than 25.0 t/ha: Nakhodka (51.1 t/ha), Skorospelka (50.7 t/ha), Sireniki (46.9 t/ha), Dieticheskiy (46.1 t/ha), Nadezhda (46.0 t/ha), Podmoskovniy (44.4 t/ha), Vylgortskiy (37.5 t/ha), Blank Brekos (33.1 t/ha), as well as five varieties of late leaf wilting: Spindle (43.9 t/ha), Korenevskiy (42.9 t/ha), Interes 21 (35.5 t/ha), Interes (27.1 t/ha), and Novost VIRa (25.2 t/ha). These data confirm that it is possible to achieve yields of Jerusalem artichoke tubers up to 54.2-58.5 t/ha [15].
To process tubers in August-September, it is preferable to use early Jerusalem artichoke varieties that, during this period, do not have time to grow and ratoon too much, and, hence, have a relatively even shape. However, at the end of October, for processing, it is preferable to use tubers of late varieties because they have already gained weight and have a mostly even shape, whereas the tubers of early varieties have already overgrown and have yeast covers that complicate their washing and peeling.

Value of Jerusalem Artichoke as a Forage Crop
One of the main objectives of agriculture development is the increase in worldwide forage production. Fodder production is certain to be a fundamental branch of agriculture. [40][41][42][43][44][45]. In feeding Jerusalem artichoke tubers and herbage to animals, it was found that these types of feed were highly attractive for all kinds of animals and preferred over other types of forage [1,[46][47][48][49][50].
Tubers are considered to be excellent succulent feed in early spring and autumn, so they are widely used in the cattle diet. Being rich in easily hydrolysable carbohydrates, mainly sugars, they provide animals with energy. The introduction of Jerusalem artichoke tubers in cows' diet increases milk yield by 4-5 kg/day after 5-6 days of feeding, and use of tubers as succulent feed for young animals significantly increases weight gain [43]. Jerusalem artichoke herbage and raw washed tubers are used as feed for domestic rabbits [45].
Analyzing the obtained data, it can be noted that, at the end of October, the herbage yield of all early Jerusalem artichoke varieties decreased by 0.5-20.7 t/ha due to the complete or almost complete drying of the aboveground part of the plant. Dry or almost dry crop tops can be used for animal feeding.
According to the nutritional value of the herbage feed in megajoules of metabolizable energy (Table 3)  It should be noted that varieties of early leaf wilting have predominance of the tuber mass in the structure of the total feed value, whereas herbage mass prevails in varieties of late leaf wilting (Figure 3). The results obtained confirmed K.P. Danilov's recommendations for Jerusalem artichoke cultivation under conditions of the Chuvash Republic and the Upper Volga region. To achieve both a high yield of tubers and vegetation conservation, harvesting of herbage mass should be carried out in mid-September, and harvesting of tubers should be undertaken in early October [12,51].

Tuber Property Analysis of Jerusalem Artichoke Varietal Resources
There are several types of Jerusalem artichoke tuber shape; for example, tubers of the varieties Vylgortskiy, Kaluzhskiy, and Sireniki are elongated; pear-shaped tubers can be found in the varieties Novost VIRa and Spindle; round-pear-shaped tubers are natural for Nakhodka, Podmoskovniy, and Blank Brekos; round-oval tubers are often found in the varieties Interest, Interest-21, Korenevskiy, Nadezhda, Tadzhikskiy, and Violet de Rense; and the tubers of Skorospelka variety are often rounded.
According to the surface indicator, the following varieties with smooth skin have been noted to be the most suitable for cultivation: Vylgortskiy, Dieticheskiy, Korenevskiy, Nadezhda, Nakhodka, Podmoskovniy, Tadzhikskiy, and Blank Brekos. Varieties with a smooth-bumpy skin surface partially meet the requirements; these are Kaluzhskiy, Novost VIRa, Sireniki, Skorospelka, and Spindle. The varieties with a bumpy-smooth skin surface, such as Interes, Interes 21, and Violet de Rense, may be more energy intensive with mechanized peeling.
Skin colors ranging from beige to brown were found in all varieties, except Tadzhikskiy (which has a reddish-light brown skin) and Violet de Rense (the tuber skin of this variety usually has a red-crimson color).
Tubers of the varieties Interes and Spindle were found to be most elongated (leggy), and about 98-107 mm long (Figure 4).
Regarding the indicator of the outermost transverse diameter, it should be mentioned that, in the first ten-day period of September, not all varieties had tubers of at least 30 mm. Tubers of Skorospelka were found to be the largest, having a diameter of more than 45 mm.
According to morphological characteristics, the most suitable varieties for fresh tuber consumption, culinary purposes, and processing were Korenevskiy, Nakhodka, Skorospelka, and Blank Brekos.
When Jerusalem artichoke tubers were harvested early, the morphological characteristics of the following varieties fully met the requirements for fresh tuber consumption, culinary purposes, and processing: Korenevskiy, Nakhodka, and Blank Brekos. Among the varieties meeting the requirements almost completely were Skorospelka, Podmoskovniy, Tadzhikskiy, Nadezhda, and Dieticheskiy.
Recyclability depends on a number of parameters, including the dry matter content. Jerusalem artichoke tubers contain up to 19-30% of dry matter, 15-20% of which is watersoluble sugars. Jerusalem artichoke tubers are also equal to potatoes in terms of nutritional value [18]. When planted on saline soils in Uzbekistan within the optimal spring period, the dry matter content in the Jerusalem artichoke tubers was 21.7-22.1%, depending on the variety studied [52].
The requirements for dry matter content during early harvesting ( Figure 6 Being rich in DM, amino acids, and carbohydrates, and low in fiber content, Jerusalem artichoke herbage can be considered to be a source of high-energy feed exceeding the nutritional value of legumes and corn [40]. A nitrate content of below 250 mg/kg was found in the following varieties: Vylgortskiy (158), Dieticheskiy (230), Interes (200), Interes 21 (121), Kaluzhskiy (208), Nakhodka (208), Sireniki (238), and Spindle (244). It was also noticed that a significantly lower nitrate content was accumulated in the part of the tuber closest to the base (stolon).
When examining tubers for disease incidence after harvesting, no diseases were found. Analyzing the data on the quality of the flesh of raw Jerusalem artichoke tubers, it can be noted that, in terms of flesh hardiness, all varieties met the requirements for raw tuber consumption and culinary purposes. Flesh browning (after 3 and 24 h) was not observed in any variety.
In terms of flesh sweetness, the most suitable varieties (with the highest point) were: Vylgortskiy, Dieticheskiy, Nadezhda, Nakhodka, and Blank Brekos.
Pleasant odor characteristics of Jerusalem artichoke, without any foreign smells, appeared in the following varieties: Interes 21, Korenevskiy, and Violet de Rense.
The raw flesh of the varieties Dieticheskiy, Nadezhda, and Nakhodka was found to have high taste qualities. Good taste was noted in the following varieties: Vylgortskiy, Interes, Interes 21, Korenevskiy, Podmoskovniy, Sireniki, Blank Brekos, and Violet de Rense (Figure 7).

Economic and Energy Efficiency of Jerusalem Artichoke Production for Foodstaff and Processing
Long-term benefits of any technology are determined by its economic efficiency [53]. It should be emphasized that Jerusalem artichoke cultivation and production efficiency in the contemporary context is of particular importance because it is a new and promising crop. According to the technological map for 1-ton production of Jerusalem artichoke for food and processing, with a 75 cm row spacing cultivation scheme, the fixed asset costs were RUB 1366.3, diesel fuel consumption was 7.4 L, gasoline consumption was 0.25 L, and electricity consumption was 6.0 kW. The labor cost of machine operators was 2.7 man-hours, that of drivers was 0.6 man-hours, and that of agriculturists was 3.4 man-hours.
In the calculation of the energy efficiency of Jerusalem artichoke varieties, it is necessary to take into account the yield obtained both of tubers and herbage. The latter is a source of high-quality animal feed and a complete green manure.
The analysis of the main energy assessment indicators of Jerusalem artichoke tubers demonstrated that, when growing varieties of early leaf wilting (compared with growing varieties of late leaf wilting), an average increase in energy consumption of 5.9 MJ/ha resulted in average growth in energy received of 18.5 MJ/ha. The energy efficiency ratio (the ratio of energy received to energy expended) improved by an average of 0.4.
The analysis of the main energy assessment indicators of Jerusalem artichoke herbage showed that, when growing early varieties (compared with growing late varieties), the amount of energy obtained was lower (on average by 20.4 MJ/ha).
Despite its low tuber yield, the highest total energy efficiency coefficient of 3.09 was obtained in the Novost VIRa variety because this variety had the highest herbage yield. The varieties of Tadzhikskiy (2.78), Spindle (2.68), and Korenevskiy (2.43) had slightly lower total energy efficiency coefficients.
This research found that the total energy costs for carrying out operations during Jerusalem artichoke cultivation were 27.4-41.3 K MJ/ha, although it is possible to achieve a total of 37.0-103.4 K MJ/ha ( Figure 8).

1.
The gross tuber yield of varieties with early leaf wilting was 33.1-51.1 t/ha, whereas that of varieties of late leaf wilting was 14.4-43.9 t/ha, including the five late varieties Spindle, Korenevskiy, Interes 21, Interes, and Novost VIRa, which had yields of 25.2-43.9 t/ha.

2.
The study showed that, for the consumption of fresh tubers at the end of August and the beginning of September, it is preferable to grow varieties with early leaf wilting. On the contrary, for application of Jerusalem artichoke tubers at the end of October, late varieties are more appropriate because tubers have already gained weight and their shapes are more even. Being harvested in late August-early September, early varieties produced, on average, 69% of marketable tubers (with a diameter more than 30 mm), whereas the marketable tuber yield of the late varieties was only 48%. For harvesting in the third ten-day period of October, the proportion of marketable tubers in the "early" varieties was 82%, and in the varieties with late leaf wilting it was 65%.

3.
The data obtained showed that it is possible to achieve a total yield of raw Jerusalem artichoke biomass of 36.8-98.1 t/ha. Moreover, in varieties of early leaf wilting, the mass of tubers prevailed in the structure of the total nutritional value of the feed (68%), and in varieties with late leaf wilting, the herbage mass was predominant (59%). 4.
Jerusalem artichoke seems to be a flexible crop that can grow on various soils and under different climatic conditions. In addition, it appears likely to be one of the most promising crops, the cultivation of which is energetically feasible. The research highlights the most future-proof varieties for cultivation in the Central Federal District of the Russian Federation, and the following varieties Nakhodka, Skorospelka, and Novost VIRa can be recommended. Funding: This research received no external funding.

Data Availability Statement:
The data is contained within the article.