Crop Establishment Methods and Integrated Nutrient Management Improve: Part II. Nutrient Uptake and Use Efﬁciency and Soil Health in Rice ( Oryza sativa L.) Field in the Lower Indo-Gangetic Plain, India

: Rice, the predominant food crop in India, is being grown traditionally with improper plant nutrient management mostly under the ﬂooded situation. Recent advancement in research on crop science focuses on water-saving rice technologies for maximization in crop and water productivity under the backdrop of a shrinking water resource base for ensuring environmental and agricultural sustainability. Under this situation, an experiment was conducted in two consecutive years in a split-plot design keeping rice cultivation methodologies, viz., aerobic culture, System of Rice Intensiﬁcation (SRI), and conventional ﬂooded culture in main plots and integrated plant nutrient management (INM) treatments in sub-plots. The experiment was aimed at understanding the effects of different rice production systems and INM on nutrient content, uptake, and use efﬁciency. The change in soil quality parameters was also studied to understand the impact of crop establishment methods (CEM) and INM options. Signiﬁcant reduction ( p ≤ 0.05) in nutrient uptake and use efﬁciency was observed under aerobic culture compared to SRI and ﬂooded method, although aerobic culture showed the highest physiological nitrogen use efﬁciency. Post-harvest available Fe status was signiﬁcantly lower in aerobic rice (mean 10.39 ppm) compared to other crop establishment technologies; however, Zn status was higher in aerobic rice over the ﬂooded situation. Although available potassium was not affected due to rice cultivation methods, available nitrogen and phosphorus status were inﬂuenced remarkably. Soil microbial quality was improved in aerobic rice in comparison to ﬂooded rice. SRI proved to be the most efﬁcient rice establishment method for enhancement in nutrient uptake, use efﬁciency, and enrichment of soil chemical and microbiological quality. Irrespective of crop culture, integrated plant nutrition in rice improved the nutrient uptake, use efﬁciency, and soil quality parameters. The study revealed that, under the alluvial soils of the Indo-Gangetic Plains of Eastern India, SRI can be considered as a water-saving rice production method. The method can also improve nutrient uptake, efﬁciency, and soil quality parameters if proper INM is adopted.


Experimental Layout
The experiment was laid out in a split-plot design with CEM in main plots and nutrient management treatments as a sub-factor, with three replicates, and individual split-plot sizes were 12 m 2 . The main plots treatments comprised C 1 : aerobic culture, C 2 : the system of rice intensification, C 3 : conventional flooded rice and sub-plot treatments consisting of absolute control (F 1 ) and the rest were F 2 : 100% recommended dose of fertilizers (RDF) through chemical fertilizers (N:P 2 O 5 :K 2 O (80:40:40)), F 3 : 75% recommended dose of nitrogen (RDN) through chemical fertilizer + 25% RDN through farm yard manure (FYM), F 4 : 75% RDN through chemical fertilizer + 25% RDN through vermicompost F 5 : 50% RDN through chemical fertilizer + 25% RDN through FYM + brown manuring (coculture of Sesbania aculeata at 30 kg/ha and knocking down with the application of 2-4 D ethyl ester at 0.75 kg a.i./ha), F 6 : 50% RDN through chemical fertilizer + 25% RDN through vermicompost + brown manuring, F 7 : F 5 + soil application of biofertilizer Azospirillium brassilense at 2 kg/ha, F 8 : F 6 + soil application of biofertilizer Azospirillium brassilense at 2 kg/ha. Details of the treatments are available in the recently published first part of this article [15].

Crop Management
Pregerminated seeds of rice were directly sown at a row spacing of 20 cm × 15 cm in case of aerobic culture, whereas 12 day-aged seedlings raised from the garden-like nursery were transplanted singly at a row spacing of 25 cm × 25 cm in case of SRI and 24 day-aged seedlings were transplanted with three seedlings per hill at a spacing of 20 cm × 15 cm in case of conventional flooded culture. The entire dose of phosphorus (40 kg/ha P 2 O 5 as single super phosphate) and potassium (40 kg/ha K 2 O as muriate of potash) were applied as basal and incorporated into soil uniformly in all the treatments at the time of final land preparation.
Fertilizer nitrogen at 80 kg ha −1 in the form of urea was applied in three splits, 1 /4 as basal, 1 /2 at maximum tillering stage, and the remaining 1 /4 at panicle initiation stage in case of the treatment where plant nutrition was supplied solely from chemical fertilizer. Under INM treatments, a proportionate amount of nitrogenous fertilizer was applied, and substituted quantity of organic sources of nutrients applied 20 days before application of chemical fertilizer. The specific strain of biofertilizer was applied along with organic sources of nutrients as per treatments. Brown manuring was practiced as per standard procedure, as detailed earlier.
Two hand weedings were performed at 25 and 40 days after transplanting. All other recommended package of practices for integrated crop management for achieving maximum productivity of the crop was followed. Ten amounts of measured quantities of Agronomy 2021, 11, 1894 4 of 17 irrigation water at 5 cm each were applied through a water meter in case of flooded culture, whereas eight amounts of irrigation water at 2.5 cm each were applied for SRI and only four amounts of irrigation water at 2.0 cm depth were applied for aerobic culture during both the years of the experiment. Sufficient drainage channels were created in aerobic plots to minimize the influence of rainfall along with suitable buffer drainage channels to be utilized to prevent seepage of water from the flooded plots to aerobic plots, and all the main plots were bounded properly to avoid movement of water and fertilizer. Details of the experimental procedures are available in the recently published first part of this article [15].

Data and their Collection Procedures
Measurements on growth and physiological attributes and yield parameters were taken as per standard procedures. The soil and plant samples after harvest of rice under different crop cultures were analyzed chemically as per the standard procedure for estimation of nutrient content, available soil nutrient status, and microbial quality, as detailed below.
Details of initial soil fertility status were mentioned in the first part of this paper. Microbial quality of soil was measured through the activity of respiratory chain enzymes soil dehydrogenase using the Triphenylformazan method as advocated by [16]. Plant analysis for nutrient content in the crops as affected by different crop culture and integrated plant nutrition was done by digestion with conc. H 2 SO 4 and distillation for total nitrogen, and for total P and K, Tri-acid digestion method was followed as described by [17].
Nutrient use efficiency was calculated for fertilizer use efficiency, i.e., agronomic efficiency and physiological nitrogen use efficiency and apparent nitrogen recovery (ANR).
Agronomic efficiency = (grain yield in the treated plot − grain yield in control plot)/amount of N fertilizer applied.
Physiological nitrogen use efficiency = grain yield/total nitrogen uptake. Apparent N recovery (ANR) = ((N uptake in N treated plot) − (N uptake in control plot)/N fertilizer applied).

Statistical Analysis
Data were analyzed for the analysis of variance (ANOVA) for split-plot design replicated experiments. The treatment effects on different parameters were tested by two-way analysis of variance. The least significant difference (LSD) test, with the level of significance set at 5%, was used to test for significant differences among treatment means [18]. Statistical procedures were carried out with the software program Statistix (Statistix Inc., Tallahassee, FL, USA) [19].

Nutrient Content and Uptake
There were no significant interactions between CEM and nutrient management treatments for nitrogen, phosphorus, and potassium content in the crops, but significant interaction effects were observed in the case of nutrient uptake by the crops. There were significant effects of crop culture and INM on total nitrogen content and uptake by the crops during both the years of the experiment (Table 1). Total nitrogen content and uptake under aerobic culture was significantly lower as compared to conventional flooded culture and System of Rice Intensification. Differences between total nitrogen content under CTR and SRI were relatively small and seldom significant; however, nitrogen uptake by the crop under SRI (131.4 kg/ha) was significantly higher over conventional culture (122.7 kg/ha). Nutrient management treatments generally caused significantly higher nitrogen content and uptake over absolute control. Total nitrogen content was higher in integrated plant nutrition compared to sole application of chemical fertilizer, although the differences were only significant under F 7 and F 8 only, where the highest values were 1.97 and 1.98, respectively. Averaged overcrop establishment methodologies and differences in nitrogen uptake among nutrient management treatments were statistically significant, though nitrogen The total N uptake by rice was significantly influenced by the combined effect of CEM and INM. However, the maximum N uptake was recorded in the system of rice intensification system for all nutrient management systems, followed by the conventional flooded transplanted rice system in both seasons. The lowest uptake was recorded in the aerobic culture system (Figure 1).
Phosphorus content in grains, straw, and total phosphorus content, as well as phosphorus uptake, was significantly higher under conventional flooded culture, compared to aerobic culture and SRI during both the years of the experiment ( Table 2).
In both years, phosphorus content and uptake under aerobic culture was lowest among the crop production technologies and perhaps significantly differed when compared to other crop production techniques. Total phosphorus content, as averaged for two years, in the crop grown under traditional flooded conditions was approximately 47.1% higher compared to that raised under aerobic culture. Phosphorus uptake by the crop in SRI was about 37.88% higher than the uptake noted under aerobic culture, whereas the magnitude of increment in uptake under flooded rice was about 44.77%. Although total phosphorus content in grain was significantly higher under nutrient management treatments over absolute control, phosphorus content in straw under the INM treatment F 5 did not differ significantly. Total phosphorus content (0.324) and uptake (47.5) under the treatment where 100% RDF was applied through chemical sources was highest, and significant differences were observed with the integrated plant nutrition treatments, viz., F 1 , F 3 , F 5 , F 6 and F 7 . The differences in phosphorus uptake and content in the integrated use of chemical fertilizer, vermicompost, brown manuring and biofertilizer with that of sole application of chemical fertilizer were nonsignificant.  Table 1; Within each treatment combination, bars followed by the same lower-case letter(s) indicate significant differences at 5% level of probability. SE± in each treatment combination was calculated from three replications.
Phosphorus content in grains, straw, and total phosphorus content, as well as phosphorus uptake, was significantly higher under conventional flooded culture, compared to aerobic culture and SRI during both the years of the experiment ( Table 2). Treatments details are in Table 1.  Table 1; Within each treatment combination, bars followed by the same lower-case letter(s) indicate significant differences at 5% level of probability. SE± in each treatment combination was calculated from three replications. Similar to N, the total uptake of P by rice was also significantly influenced by the combined effect of CEM and INM. However, with little exception, significantly similar, and the maximum, P uptake was recorded in both conventional flooded transplanted rice system and SRI system for all nutrient management systems in both years. However, the lowest uptake was recorded in the aerobic culture system for all nutrient management treatments ( Figure 2). differences were observed with the integrated plant nutrition treatments, viz., F1, F3, F5, F6 and F7. The differences in phosphorus uptake and content in the integrated use of chemical fertilizer, vermicompost, brown manuring and biofertilizer with that of sole application of chemical fertilizer were nonsignificant. Similar to N, the total uptake of P by rice was also significantly influenced by the combined effect of CEM and INM. However, with little exception, significantly similar, and the maximum, P uptake was recorded in both conventional flooded transplanted rice system and SRI system for all nutrient management systems in both years. However, the lowest uptake was recorded in the aerobic culture system for all nutrient management treatments ( Figure 2).

Figure 2.
The combined effect of crop establishment methods and integrated nutrient management influence the total P uptake by rice in both years. Treatment details are in Table 1; Within each treatment combination, bars followed by the same lower-case letter(s) indicate significant differences at 5% level of probability. SE± in each treatment combination was calculated from three replications.
As far as potassium content and uptake is concerned, aerobic culture had significantly lower nutrient content and uptake, compared to two other crop production techniques (Table 3). Potassium content and uptake under the SRI and traditional flooded culture was nonsignificant and inconsistent. Potassium uptake under 100% RDF through chemical fertilizer and integrated plant nutrition package, as followed in F4 and F8, did not differ significantly, whereas other nutrient management treatments had a significant impact on potassium content and uptake. There were no statistical differences in potassium content and uptake among F3, F5, and F6.  Table 1; Within each treatment combination, bars followed by the same lower-case letter(s) indicate significant differences at 5% level of probability. SE± in each treatment combination was calculated from three replications.
As far as potassium content and uptake is concerned, aerobic culture had significantly lower nutrient content and uptake, compared to two other crop production techniques (Table 3). Potassium content and uptake under the SRI and traditional flooded culture was nonsignificant and inconsistent. Potassium uptake under 100% RDF through chemical fertilizer and integrated plant nutrition package, as followed in F 4 and F 8 , did not differ significantly, whereas other nutrient management treatments had a significant impact on potassium content and uptake. There were no statistical differences in potassium content and uptake among F 3 , F 5 , and F 6 .
Similar to N and P, the total uptake of P by rice was also significantly influenced by the combined effect of CEM and INM. Although nutrient management was varied in different CES systems, significantly similar, and the maximum, K uptake was recorded in SRI and conventional flooded transplanted rice systems in both years. Aerobic rice cultural system always showed the minimum uptake of K compared to the other two systems (Figure 3).

Nutrient Use Efficiency
Significant interaction effect between crop culture and INM was observed for fertilizer use efficiency during both the years of the experiment (Table 4). Fertilizer use efficiency or agronomic efficiency of rice recorded under this experiment was significantly higher in SRI (5.93) compared to aerobic culture (3.81) and conventional flooded culture (5.27). Fertilizer use efficiencies of the crop under all INM treatments were numerically higher, as compared to 100% RDF, and significant differences were noted for all integrated plant nutrition treatments except F 5 and F 6 . Treatments details are in Table 1.
Similar to N and P, the total uptake of P by rice was also significantly influenced by the combined effect of CEM and INM. Although nutrient management was varied in different CES systems, significantly similar, and the maximum, K uptake was recorded in SRI and conventional flooded transplanted rice systems in both years. Aerobic rice cultural system always showed the minimum uptake of K compared to the other two systems (Figure 3).  Table 1; Within each Figure 3. The combined effect of crop establishment methods and integrated nutrient management influence the total K uptake by rice in both years. Treatments details are in Table 1; Within each treatment combination, bars followed by the same lower-case letter(s) indicate significant differences at 5% level of probability. SE± in each treatment combination was calculated from three replications. Table 4. Crop establishment methods and integrated nutrient management influence the nutrient use efficiency, partial factor productivity of nutrients, value cost ratio, and apparent nitrogen recovery in rice. Physiological nitrogen use efficiency of rice ranged from 47.33 kg grain kg −1 of nitrogen uptake in the case of SRI to 54.26 kg grain kg −1 of nitrogen uptake in the case of aerobic rice. There was no significant interaction between crop establishment methodologies and INM for physiological nitrogen use efficiencies of the crop during 2014-2015 and 2015-2016. Physiological nitrogen use efficiency of rice was highest under 100% RDF and tended to decrease in the case of INM treatments, although the differences were marginal and not statistically significant, except the nutrient management treatment F 8 where 50% RDN was substituted and vermicompost, brown manuring, and a specific strain of biofertilizer were applied. Significant interaction effects between crop culture and nutrient management treatments were observed in the case of apparent nitrogen recovery (ANR) values of the crop. Apparent nitrogen recovery of rice under aerobic rice culture was significantly lower (0.82) compared to flooded rice, and SRI registered the highest numerical apparent nitrogen recovery value (1.41), whereas the difference in ANR values between SRI and conventional flooded rice was marginally small and nonsignificant. Apparent nitrogen recovery values in rice under integrated plant nutrition, irrespective of crop culture, was significantly higher when compared to absolute control and 100% RDF. The highest ANR value (1.41: pooled for two years) was achieved under integrated use of chemical fertilizer, brown manuring vermicompost, and application of Azospirillium brassilense, i.e., F 8, which also significantly differed from other integrated plant nutrition treatments. The ANR values ranged from 0.57, in the case of absolute control, to 1.41, in the case of INM treatment (F 8 ).

Effect on N, P, and K Status
The interaction effect between CEM and INM was found to be nonsignificant in relation to available N, P, and K status of soil after harvest of the crop (Table 5). However, available nitrogen status of 326 kg/ha and 287 kg/ha, respectively, during the consecutive two years of the experiment was highest under SRI, which significantly differed from available nitrogen status recorded under aerobic culture and conventional transplanted rice. The lowest numerical values of available nitrogen status of 293.8 kg/ha 260.3 kg/ha, as registered under aerobic culture, were attributable to loss of higher magnitude of nitrogen due to more losses through the nitrification-denitrification process. All the nutrient management treatments significantly improved the available nitrogen status in soil over control, but the highest available nitrogen status was observed under INM treatment F 7 with mean available nitrogen status of 297.7 kg/ha, which was significantly different from F 1 , F 2 , F 3 , F 4 , F 5 , and F 6 , but on par with F 8 .
Available phosphorus status in soil was significantly affected by CEM and INM. The highest available phosphorus statuses of 80.9 kg/ha and 67.7 kg/ha, respectively, were observed under conventional transplanted rice, which significantly differed from phosphorus status recorded under aerobic culture but were on par with the values registered under SRI. Naturally, the available phosphorus status in soil was significantly lower under control during both the years of the experiment when compared with the nutrient management practices. Although the highest available phosphorus status was noted under INM treatment F 7 , the treatment was on par with other nutrient management practices.
Similar to the phosphorus status of soil, the highest available potassium status was observed under conventional transplanted rice, which was significantly higher than the nutrient status as observed under aerobic culture and System of Rice Intensification. The highest available potassium status of 276.3 kg/ha and 267.6 kg/ha, respectively, were recorded under F 2 treatment (100% RDF) during the consecutive two years of the experiment, which significantly differed from F 1 (absolute control) only, while other INM treatments remained on par.

Effect on Soil pH, Organic Carbon, and Micronutrients
The pH values recorded under the experiment did not show any significant variation in CEM and INM during both the years of the experiment ( Table 6). The lowest pH values of 6.81 and 6.90, respectively, under aerobic culture, and highest corresponding pH values of 7.07 and 7.19, respectively, during 2014-2015 and 2015-2016 were noticed under conventional transplanted rice as soil pH approached neutral equilibrium due to submergence, whereas soil pH under aerobic situation could not approach neutrality due to positive soil redox potential. Significant and considerable changes in pH also were not observed among the nutrient management treatment and absolute control. The organic carbon status of soil was significantly influenced both by CEM and INM. SRI exhibited significantly higher organic carbon status over aerobic culture, but organic carbon status recorded under conventional transplanted rice was on par with SRI. Significantly higher organic carbon status was registered in all nutrient management treatments over control. The highest organic carbon contents of 0.74% and 0.78%, respectively, during 2014-2015 and 2015-2016 were observed under INM treatment F 8 , differing significantly from F 1 , F 2 , F 3 , F 4 , F 5 , F 6, and F 7 . Interestingly, the treatments that received 50% RDN through chemical fertilizer, 25% RDN through either FYM or vermicompost, brown manuring, and biofertilizer, i.e., F 7 and F 8, registered higher organic carbon status over the rest of the treatments owing to the synergistic effect of inorganic fertilizer, organic sources, and biofertilizer in a synchronized manner. A relatively lower magnitude of organic carbon was noticed under F 2 , where 100% RDN was applied through chemical fertilizer. The highest available iron status of 13.44 parts per million and 14.46 parts per million, respectively, was recorded in the soil after harvest of rice under the conventional transplanted condition that significantly differed from aerobic culture and system of rice intensification. Available iron status after harvest of rice under aerobic culture was only 10.70 parts per million and 10.09 parts per million, respectively, during 2014-2015 and 2015-2016. Among INM treatment, F 6 registered the highest available iron status in soil, with the numerical mean value of 13.52 parts per million, which significantly differed from F 1 , F 2 , F 3 , and F 4 , but was on par with F 5 , F 7 , and F 8 . Astonishingly, higher available Fe status was observed in the INM treatments where brown manuring was practiced.
Available Zn status in soil was significantly higher under aerobic culture, where 1.46 parts per million and 1.49 parts per million (ppm) Zn was recorded during 2014-2015 and 2015-2016, respectively, which was significantly higher than available Zn status as recorded under SRI and conventional flooded rice. INM treatment F 3 recorded the highest available Zn status of 1.39 ppm and 1.43 ppm, respectively, which significantly differed from F 1 and F 2 ; however, the rest of the treatments were on par. Higher available Zn status, as observed under treatments receiving integrated plant nutrition, may be due to the effect of organic matter on chelation. However, the interaction effect of CEM and INM was not significant in respect to pH, organic carbon, and available micronutrients (Fe and Zn) status of the soil.

Effect on Soil Dehydrogenase Activity
Soil dehydrogenases are respiratory chain enzymes generally present in every upper layer of soils, and activities of the enzymes are generally used as an indicator of the biological redox system as a measure of microbial activity in the soil, and thus are used to assess microbial soil quality. Although the interaction effect of CEM at different INM was found to be nonsignificant during both the years of the experiment, soil dehydrogenase activities at different growth stages of crop was significantly affected by CEM and INM (Table 7). Significantly higher soil dehydrogenase activity was observed under SRI over other CEM at tillering stage. Conventional transplanted rice culture registered the lowest values of soil dehydrogenase activities among the CEM but was on par with aerobic culture. Significantly higher dehydrogenase activities were noticed under all nutrient management treatments, compared to absolute control where no nutrient was supplied to rice crop. The highest values of soil dehydrogenase activities of 179.0 and 173.2 microgram Triphernylformazan (TPF)/g soil/h during 2014-2015 and 2015-2016, respectively, were found in INM treatment F 4 , which significantly differed from F 1 , F 2 , F 5 , F 6 , F 7 , and F 8 at this stage of the crop.
Irrespective of crop culture and INM practices, the soil dehydrogenase activities reached at its peak at the flowering stage. During the flowering stage of the crop, soil dehydrogenase activity, as observed under aerobic culture, was 194.8 and 186.2 microgram TPF/g soil/hour, respectively, in 2014-2015 and 2015-2016; in SRI, the corresponding values were 205.3 micrograms TPF/g soil/hour and 191.4 micrograms TPF/g soil/hour, respectively, whereas conventional transplanted rice recorded the lowest enzymatic activities of 187.3 micrograms TPF/g soil/hour and 179.3 micrograms TPF/g soil/hour, respectively. All INM treatments significantly enhanced soil dehydrogenase activities in soil, as compared to absolute control and application of 100% recommended dose of chemical fertilizer. F 8 treatment, which received integrated use of chemical fertilizer, vermicompost, brown manuring, and biofertilizer, recorded the highest soil dehydrogenase activities of 217.4 micrograms TPF/g soil/hour and 209.3 micrograms TPF/g soil/hour during 2014-2015 and 2015-2016, respectively, and this treatment was immediately followed by F 7 . As the crop progressed to maturity and harvest, the soil dehydrogenase activities declined progressively under different CEM and INM. At harvest, the INM treatments also registered significantly higher soil dehydrogenase activities than absolute control and the treatment where 100% RDF was applied.

Discussion
Nutrient content, uptake, and use efficiency were lower [20] under aerobic culture compared to other crop establishment methodologies due to loss of nutrients within the agro-ecosystem and environment. SRI, having potential for better resource use, proved to be the most efficient waterwise rice production technology for better nutrient content, uptake, and use efficiency, and conventional flooded culture was intermediate between SRI and aerobic culture in terms of nutrient content, uptake, and use efficiency. The growth of aerobic rice and nitrogen uptake can be improved through soil acidification. There should be site-specific technology for aerobic culture regarding nutrient management for nutrient use efficiency and yield enhancement. When urea is applied at seeding, urea-induced ammonia toxicity causes poor growth of aerobic rice [21,22], and monocropped aerobic rice also experiences a poor growth pattern due to loss of ammonia after urea application in high pH conditions due to ammonia volatilization. Selection of the right nitrogen source, such as ammonium sulphate, where volatilization occurs at a less constant rate compared to urea, significantly increases growth parameters of aerobic rice [23], reduces soil pH up to 6.0, and increases plant micronutrient content (Fe, Mn, Zn) and plant growth. Application methodologies such as the deep placement of urea, at a depth of 5.0 cm in soil, significantly reduce nitrogen loss by ammonia volatilization and slow-releasing nitrogen. Reduced soil pH induced by the change in soil redox under aerobic situations was observed from the experiment. Reduction in soil pH under aerobic soil due to acidification is reported by Kreye et al. [24], and the situation is relevant in the context of continuous cropping obstacles of aerobic culture, also documented by different researchers. The lowest magnitude of organic carbon as observed under aerobic culture may be due to the loss of organic matter induced by rapid decomposition.
Nutrient content, uptake, and use efficiency under integrated plant nutrition, irrespective of crop culture, was higher compared to sole application of chemical fertilizer, owing to better availability of nitrogen due to application of organic sources and biofertilizer that ultimately triggered better nitrogen content both in grains and straw [25]; however, phosphorus content and uptake that was relatively lower under INM treatments, compared to sole application of chemical fertilizer, may be due to the reason that phosphorus is released slowly under organic sources of nutrients, and amount of nutrients contained in it may not be available during single crop season, and residual beneficial effect may be reflected in the cropping system.
Available nitrogen status was observed as higher under INM treatments F 7 and F 8 due to conjunctive use of chemical fertilizer, FYM/vermicompost, brown manuring, and biofertilizer, which maintained higher magnitude of available nitrogen status in soil even after crop uptake from soil. This combination of INM treatments perhaps ideally fitted for a better harvest of the crop and maintaining soil fertility, whereas the relatively lower magnitude of available nitrogen status was recorded under F 2 , i.e., treatment receiving sole application of chemical fertilizer owing to maximum removal by the crop within the current season without sufficient replenishment within the soil from other sources of nutrients or soil nutrient pool. Higher phosphorus status under flood-irrigated rice may be due to increased availability of Fe-phosphates and occluded phosphorus under submerged conditions, and as a consequence of flooding, reduction of Fe 3+ and Mn 4+ ions take place, their concentration in soil solution is increased, and these ions exchange with K on exchange complex of clay minerals leading to increased availability of native potassium. This explanation of the increased availability of K under flooded rice culture is also supported by Nad and Goswami [26]. Higher available K status recorded under F 2 over INM treatments may be due to better availability and release of potassium under chemical fertilizer in current cropping season as release pattern of nutrients from organic sources are relatively slow, for which the impact may be realized on long-term perspective during succeeding cropping season.
Aerobic soil is diverse in nature. Soil pH under conventional puddle-transplanted rice approaches neutral equilibrium, which offers great opportunities for rice cultivation under varied agro-ecosystem. Moreover, conventional puddle-transplanted rice has a beneficial effect on soil chemical fertility, favoring better availability of nutrients, organic matter accumulation, and biological N fixation to supplement the crop's additional N [27], but aerobic rice soil cannot approach neutrality for positive redox potential, deterring the availability of Fe and Mn. Rice plant in aerobic soil suffers from inadequacy in mineral nutrition as nutrient transport to soil via mass flow and diffusion slow down due to reduced soil moisture regime and higher heat capacity of soil moisture.
As envisaged from the experimental result, the shift in rice culture to the aerobic situation recorded the lowest available Fe status in soil. Lower availability of iron, and iron deficiency, as the common nutritional problem is also documented by Rong-li et al. [28], where it was established that amounts of phytosiderophores released from aerobic rice do not increase under Fe deficiency condition, and ferric ion reducing capacity does not increase. The experimental findings are very much relevant in the pretext of promoting water-saving aerobic rice technologies in rice-based triple cropping systems under the scenario of shrinking water resource base, where special attention of Fe nutrition in rice is to be assured to avoid Fe malnutrition in crop, as well as in the human food chain. Foliar application of Fe is one of the current crop management strategies in aerobic rice [29], besides soil application, cropping system, and appropriate breeding strategies for iron-enriched rice crops and varieties for improving nutritional qualities and biofortification for benefit of human civilization to alleviate human micronutrient malnutrition [30]. Integrated crop management strategies overcome the problem of Fe deficiency in rice. The integrated plant nutrition treatment where brown manuring was included as a component exhibited relatively higher available Fe status in soil. This finding has close proximity with that of Yadav et al. [6], where aerobic rice culture with Sesbania mulch and iron fertilization increased the production of aerobic rice with adequate iron nutrition and available Fe status. However, the role of Sesbania mulch in aerobic rice for enhancing yield or available Fe status in soil after harvest of crop needs to be studied for final confirmation of conclusion. With respect to available Zn status in soil, significantly higher available Zn status was recorded under aerobic culture over the other two CEM. The findings partly corroborate those of Saha et al. [31], where the significant change of micronutrient status was observed up to 90 DAS stage, but after harvest, although numerical values were higher, the difference was nonsignificant.
Soil dehydrogenase activity assesses microbial soil quality and depends on the intensity of biological conversion of organic compounds. Relatively fewer dehydrogenase activities, as recorded under conventional flooded rice during tillering and flowering stage over other CEM, may be due to slower enzymatic activities under anaerobic situation which prevailed under submergence. Higher dehydrogenase activities, as observed under INM treatment, were due to enhanced microbial activities due to brown manuring and application of organic matter [32] and biofertilizer, as activity of dehydrogenase depends on the metabolic activity of soil biota and significantly correlates with soil biomass carbon in organically amended soil [33].
There is no doubt that SRI is one of the best options in terms of soil health, nutrient efficiency, and nutrient uptake where a controlled irrigation facility is available, but under the submerged and partially flooded conditions of lower IGP, the conventional flooded method seems to be the most feasible method for farmers. However, for the limited and controlled water conditions, the aerobic method can be suitable if specially bred cultivars are adopted. The study clearly revealed that for sustainable rice cultivation under an intensive cropping system of the lower IGP, the practice of INM should be adopted, targeting soil quality and nutrient use efficiency. Furthermore, for INM, based on local availability, the sources of organic nutrients can be chosen preferably with biofertilizer.
The crop performance of rice under different establishment methods may vary with variety and micro-situation. Hence, there is enough scope for testing different varieties under specific situations considering the vastness of the lower IGP. The quality parameters of an organic source of nutrients and biofertilizer are not accurately maintained, which may bring inconsistency in results. Furthermore, different biofertilizer application methods (namely, seed inoculation, seedling dip, and soil application) can be studied to find the efficiency under different rice establishment methods. In this regard, other biofertilizers can also be tested alone or as consortia.

Conclusions
For optimization of crop and water productivity, water-saving rice technologies are becoming inevitable in the tropics as a paradigm shift under the backdrop of shrinking water and other resource bases. However, the shift in production technologies have some adverse impacts on nutrient uptake, use efficiency, crop quality, and some micronutrient deficiencies, especially Fe, under the aerobic situation that may pose a serious threat to Fe malnutrition in the cereal-based food chain if aerobic rice is continuously cropped in rice-based multiple cropping systems. However, under the alluvial soils of the Indo-Gangetic Plains of Eastern India, SRI is still proved to be an environmentally benign water-saving production method in terms of nutrient uptake and efficiency, as well as soil quality parameters, if proper INM is followed. Interestingly, brown manuring, biofertilizer, and other locally available bio-resources are gaining importance in plant nutrition under alternative rice production technologies. As a consequence of such a situation, suitable crop management strategies to be chalked out, encompassing crop improvement, nutrient biofortification, rational and integrated soil and nutrient management, and long-term cropping system management, which are going to be indispensable before promoting waterwise and water-saving rice production methodologies.