Mitigation of High-Temperature Damage by Application of Kaolin and Pinolene on Young Olive Trees (Olea europaea L.): A Preliminary Experiment to Assess Biometric, Eco-Physiological and Nutraceutical Parameters

Various products are used to mitigate the negative effects of abiotic stress in olive trees. The aim of the research was to examine an anti-transpirant product (Vapor Gard®, V) and a kaolinbased product (Manisol, K) effect on the growth of two-year-old olive tree seedlings under high temperature. The study was conducted in a greenhouse on trees of a native cultivar of Campania (cv. Salella) grown in pot during the growing season from May to September 2020. The experimental design included two products: di-1-p-menthene (product V) and kaolin (product K), applied five times at 20 day intervals compared with a control. The following biometric, physiological, and nutraceutical parameters were evaluated: stomatal conductance, chlorophyll a fluorescence, Soil Plant Analysis Development (SPAD) index, relative water content (RWC), shoots growth, total leaf area per plant, trunk cross-sectional area, dry matter partitioning, total polyphenols, and antioxidant activity. The results obtained showed that the application of di-1-p-menthene (V) was able to induce a significant improvement of shoots growth (+37.22%) and trunk cross-sectional area (+46.60%) and a reduction of the stomatal conductance and an increase of leaf RWC values. Application with kaolin had positive effects on the total polyphenol content, with an increase over the control of 240.33% and higher antioxidant activity values. Further studies are necessary to determine the effect of these products on the biometric, physiological and nutraceutical parameters of mature olive trees cultivated in open field conditions.


Introduction
The olive tree (Olea europaea L.) is one of the most important crops in the Mediterranean basin, and its growth and development are mainly controlled by atmospheric conditions [1,2]. In recent years, with the scenario of climate change, this cultivar is strongly exposed to thermal and hydric stresses during the growing season, mostly during summer and in the innermost areas of Europe [3]. These changes in temperature and precipitation along with a greater frequency of extreme weather have reduced agricultural yield [4,5]. Temperature affects most plant physiological processes, including photosynthesis and transpiration, which are both regulated by stomatal conductance and which mutually affect each other [6]. The atmospheric CO 2 concentration is increasing, and in addition to its direct effects on plant growth, this change is expected to raise the global mean surface temperature and result in an increase in the severity of summer drought [7]. The olive tree (2) Kaolin (K), the product used was Manisol (Kaolin + Copper 5%) from Manica S.p.a (Rovereto, Italy), which was applied by foliar application with 80 g in 2 L of water; (3) Di-1-p-menthene (C 20 H 34 ) (V), a terpenic polymer also known as pinolene, in the form of a water emulsifiable organic concentrate. The product used was Vapor Gard ® from Biogard ® (Bergamo, Italy), which was applied by foliar application with 20 ml in 2 L of water.
The two treatments were sprayed using a portable pump. Plants were irrigated with water equal to 100% of the evapotranspiration (ET), calculated by weight. A drip irrigation was considered by placing two drippers (2 L/h) per plant. The system was fed by electric pumps, and irrigation was automatized due to a timer. For each treatment, ten technical replicates were utilized, each consisting of a single tree.

Biometric and Physiological Analysis
During the growing season, the following measurements were performed: (i) shoots growth with a digital caliber (Borletti CDJB20 accuracy 0.03 mm, Antegnate (BG), Italy), (ii) the circumference of the trunk at 3 cm above the soil, in order to determine the increase in the trunk cross-sectional area (TCSA) calculated by standard formula (girth 2 /4π), and (iii) leaf number per plant to determine leaf area per plant using imageJ software version 1.50 (Wayne Rasband, National Institute of Health, Bethesda, MD, USA).
On fully developed leaves, the stomatal conductance was measured using a Porometer (Li-1600 Steady State Porometer, Lincoln, NE, USA) taking 6 measurements per treatments; leaf soil plant development (SPAD) index was assessed by taking 20 measurements per treatments using a chlorophyll meter SPAD-502 (Konica-Minolta, Osaka, Japan). Plant water status was assessed by calculating the leaf relative water content (RWC) as follows. Immediately after sampling, leaves were placed in plastic bags inside a cooler box and transferred to the laboratory where the fresh weight (fw) was recorded. Due to the thick cuticle/epidermal layers, the sampled leaves were then rehydrated for 48 h in distilled water at 4 • C in the dark prior to measuring the rehydrated weight (rw). Subsequently, the same leaves were dehydrated for at least 48 hours at 80 • C until constant weight was reached, before their dry weight (dw) was measured. RWC (%) was calculated as: These eco-physiological measurements were performed three times during the trial: on 29 June, 21 July, and 25 August.
Before the end of the experiment, chlorophyll a fluorescence measurements were recorded in the field on 30 min dark-adapted leaves using a PAR-FluorPen FP 110/D portable fluorimeter (Photon Systems Instruments, Drásov, Czech Republic) equipped with detachable leaf-clip. Measurements occurred at 9 a.m. and 1:30 p.m., for a one hour lap each, according to the procedure reported by Mola et al. [29]. Fluorescence data were analyzed by the FluorPen software ver. 1.1 (Photon Systems Instruments, Drásov, Czech Republic), and the F v /F m parameter was calculated as follows: where F 0 is the basal fluorescence recorded at 40 µs and F m is the peak of the fast fluorescence rise following illumination of the dark-adapted leaves with a saturating flash of light. At the end of the growing season, the plants were harvested to determine the dry matter partitioning of the various components of the trees (roots, leaves, wood and total). Then, the root/canopy ratio of the individual plants was calculated. At the end of the season, six samples of leaves per treatment were frozen and freeze-dried to determine the polyphenol content and antioxidant activity.

Ultrasound-Assisted Extraction of Polyphenolic Compounds
Lyophilized samples were extracted using the method reported in the literature [31] with few modifications. In particular, 0.3 g of dried sample was extracted with 15 mL of methanol/water (80:20 v/v, 0.1% formic acid) by sonication at room temperature for 15 min. Samples were centrifuged to 4000 rpm at 4 • C for 10 min, and the pellets were extracted again in the same way. The supernatants were collected, filtered through 0.45 mm nylon syringe membranes, and then used for high-resolution mass spectrometry analysis and antioxidant activity assay.

UHPLC-HRMS Analysis of Polyphenolic Compounds
An Ultra-High-Pressure Liquid Chromatograph (UHPLC, Dionex UltiMate 3000, Thermo Fisher Scientific, Waltham, MA, USA) coupled with a Q-Exactive Orbitrap mass spectrometer (UHPLC, Thermo Fischer Scientific, MA, USA) was used to investigate the quali-quantitative profile of polyphenolic compounds, by applying the conditions reported in our previous work [32]. All results are expressed as µg/g dw of olive leaves.

Antioxidant Activity: ABTS Assay
Determination of the ABTS free radical scavenging activity was conducted following the method described by Re et al. [33].

Antioxidant Activity: DPPH Assay
The DPPH assay was conducted according to the procedure reported by Brand-Williams et al. (1995) [34] with minor modifications. Briefly, methanolic DPPH radical working solution was prepared diluting methanolic DPPH (4 mg in 10 mL) with methanol until an absorbance value of 0.900 ± 0.020 at 517 nm. For the assay, 200 µL of sample were added to 1 mL of radical working solution, and the absorbance value was monitored after 10 min. The results were expressed as Trolox equivalents antioxidant capacity (TEAC, mmol Trolox equivalent kg −1 of dry weight of sample). All determinations were performed in triplicate.

Statistical Analysis
Analysis of variance (two-way ANOVA) was applied to analyze the group means. Duncan's multiple range test was (DMRT) performed for mean separation of each of the significant (p < 0.05) measured variables. A principal component analysis (PCA) was executed on biometric, physiological and nutraceutical parameters at the end of growing season, to detect the interrelationship. The statistical package XLSTAT Version 2013 (New York, NY, USA) was implemented for all the analyses.

Implications of Kaolin and Pinolene for Biometric and Physiological Parameters
Mean, maximum and minimum air temperature inside the greenhouse during the cultivation cycle are presented in Figure 1. The maximum temperatures of 51.74 • C in July and 49.90 • C in August were recorded, while the minimum temperature was 23.42 • C in June ( Figure 1). Figure 2 shows the effect of Kaolin (K) and di-1-p-menthene (V) products on shoots growth of olive plants compared with control (C) during the growing season. No significant difference was shown between the various treatments; on the contrary, a significant difference was highlighted during the days of growing season for the shoots growth. The interaction between treatments and days of measurements (T × D) was found to be signifi-Agronomy 2021, 11, 1884 5 of 14 cant with p < 0.001. At the end of the growing season, V applications improved the growth of the shoots compared to C by about +46.60%. On the contrary, K application did not significantly improve the performance of this parameter with respect to the C (+10.70%). Mean, maximum and minimum air temperature inside the greenhouse during the cultivation cycle are presented in Figure 1. The maximum temperatures of 51.74 °C in July and 49.90 °C in August were recorded, while the minimum temperature was 23.42 °C in June ( Figure 1).

Figure 1.
Monthly temperature trend: maximum, minimum, and mean temperature (°C) recorded during the growing season under the greenhouse. Figure 2 shows the effect of Kaolin (K) and di-1-p-menthene (V) products on shoots growth of olive plants compared with control (C) during the growing season. No significant difference was shown between the various treatments; on the contrary, a significant difference was highlighted during the days of growing season for the shoots growth. The interaction between treatments and days of measurements (T×D) was found to be significant with p < 0.001. At the end of the growing season, V applications improved the growth of the shoots compared to C by about + 46.60%. On the contrary, K application did not significantly improve the performance of this parameter with respect to the C (+ 10.70%).    Figure 2 shows the effect of Kaolin (K) and di-1-p-menthene (V) products on shoots growth of olive plants compared with control (C) during the growing season. No significant difference was shown between the various treatments; on the contrary, a significant difference was highlighted during the days of growing season for the shoots growth. The interaction between treatments and days of measurements (T×D) was found to be significant with p < 0.001. At the end of the growing season, V applications improved the growth of the shoots compared to C by about + 46.60%. On the contrary, K application did not significantly improve the performance of this parameter with respect to the C (+ 10.70%). At the end of the growing season, V treatment resulted in significantly higher values of TCSA, in comparison with C (+34.7%) and K treatment (+41.7) (Table 1), in addition to shoots growth being only significantly higher under V treatment, 37.2% compared to C. Meanwhile, leaf area per plant showed significant differences when plants were treated with K and V with respect to C, registering an increase of about +10.2% and +9.4% respectively. Our results are consistent with those reported previously by Roussos et al. (2010) [35], where plant height in olive trees with kaolin applications did not report significant differences with respect to control. Similar results to ours were also reported by MI et al. [36], where in a test on Ficus carica L., the use of two anti-transpirant types, namely "Folicote" and Vapor-gard, increased growth parameters, yield and fruit quality in the first season. The anti-transpirants products are expected to reduce photosynthesis and hence the growth of the plants. However, since the anti-transpirants close the stomata, the plant water potential increases, and it is possible to induce an increase in growth, despite reduced photosynthesis [37]. Table 1. Effect of kaolin (K) and di-1-p-menthene (V) on shoots growth, trunk cross sectional area (TCSA) and leaf area per plant at the end of growing season. C: control.

Treatments
Shoots The effect of kaolin (K) and 1-p-menthene (V) applications on eco-physiological parameters are reported in Table 3, after one (29-Jun), two (21-Jul) and three (25 Aug) months of the first application (25 May). Figure 3a shows the stomatal conductance values, where already after one month from the first application, the effects of the treatments were evident.  Another parameter used for evaluating the state of plants was the relative water content index (RWC) (Figure 3b); in this case, no significant difference was shown between the various treatments, but a significant difference was highlighted during the days of growing season. The interaction between treatments and days of measurements (T × D) was found to be significant with p < 0.01. In particular, in June, no statistically No significant difference was shown between the various treatments; on the contrary, a significant difference was highlighted during the days of growing season for the stomatal conductance. The interaction between treatments and days of measurements (T×D) was found to be significant with p < 0.001; conductance values with C applications were equal to 0.53 mol −2 s −1 , significantly higher than the treated plants. During the vegetative season in July, K applications showed an increase in stomatal conductance (0.56 mol −2 s −1 ), but at the end of the vegetative season, all three treatments showed low conductance values, in particular K, which was statistically different from C with a reduction of 3.70%. Similar results have also been observed by Di Vaio et al. [38,39] on "Aglianico" and "Falanghina" (Vitis Vinifera L.), where a product based on 1-p-menthene significantly reduced the stomatal conductance and assimilation rate. Stomatal conductance usually decreases at high temperatures; however, this result is probably due to leaf water deficit or large leaf-to-air water vapor concentration differences generated by high temperature [40,41].
Another parameter used for evaluating the state of plants was the relative water content index (RWC) (Figure 3b); in this case, no significant difference was shown between the various treatments, but a significant difference was highlighted during the days of growing season. The interaction between treatments and days of measurements (T × D) was found to be significant with p < 0.01. In particular, in June, no statistically significant differences were recorded, as the plants had not yet undergone prolonged thermal stress; in July, only treatment with V reported significant different values, about +26.39% higher than C. In August, the effect of stomatal closure of the two spray products was clearly highlighted, as they showed significantly higher values of RWC, about +15.8% in K and +27.87% in V compared to C. Anti-transpirants are chemical compounds used to limit the transpiration process and to maintain the advantageous parameters of the water balance of plants [42], and they are commonly used to reduce leaf water loss. For instance, anti-transpirant products increased the relative water content of Morus alba leaves at different irrigation levels [43]. They may allow a reduction in water transpiration without greatly affecting photosynthetic activity [44]. In addition to the beneficial effect of elevated CO 2 concentration on drought stress, anti-transpirants can significantly improve drought tolerance [45]. Our results are in agreement with Mikiciuk et al. [46], where the antitranspirant-used product increased the RWC in leaves of the tested strawberry cultivar by 4.4%. According to Abdel-Fattah [47], anti-transpirants, which form a film on the surface of the plants, increase the RWC in leaves.
Plants treated with both products had an F v /F m of 0.81 at 09:00 a.m., corresponding to the optimum value for healthy, non-stressed plants [48], while plants treated with K showed an F v /F m of 0.79 always at 09:00 a.m. (Figure 3c).
Significant differences were shown between the various treatments and during the time of measurements. The interaction between temperature and time of measurements (T × H) was found to be significant with p < 0.001. The F v /F m decreased gradually and at the mid-day measurement; it was significantly lower than that registered at 09:00 a.m. thus evidencing the daily fluctuation in the maximum quantum yield of PSII photochemistry. At 02:00 p.m., significant differences emerged between C (F v /F m = 0.76), K (F v /F m = 0.73) and V (F v /F m = 0.79) plants. The V treatment reduced the mid-day depression in photosynthetic efficiency by narrowing the daily fluctuation in F v /F m , while the K treatment increased this depression. Overall, this positive effect of V application on the plant photosynthetic metabolism may explain the higher growth parameters recorded for plants treated with this anti-transpirant. Our F v /F m results with K applications are in contrast with Segura-Monroy et al. [49] findings. The authors noted significant differences in F v /F m ratio due to kaolin treatments in Physalis peruviana L., with untreated plants having reduced quantum efficiency (with kaolin (0.83) vs. without kaolin (0.79)). Our results are consistent with those reported previously by Latocha et al. [50], where anti-transpirant product application increased the chlorophyll content in leaves at the beginning of the experiments, and it enhanced the efficiency of the photosynthetic apparatus during almost the whole experimental period on Actinidia arguta.
Data on the degradation of the leaf chlorophyll molecule after thermal stress conditions were recorded using a Minolta chlorophyll meter SPAD during the vegetative season, showing a significant difference between the various treatments. The interaction between treatments and days of measurements (T × D) was found to be significant with p < 0.05. Our results showed, at the end of the test, positive effects of K and V products in maintaining higher levels of leaf SPAD than C (Figure 3d). K induced an increase in SPAD index equal to +6.8% and V equal to +6% compared to C. Higher F v /F m chlorophyll fluorescence emissions and SPAD index values on anti-transpirant trees compared with C, indicating less damage of the photosynthetic apparatus and higher leaf chlorophyll content.
As chlorophyll is the major component of photosynthetic activity [51], it may explain yield increase at harvest in polymer-treated trees due to the improvements in these two measurements. Nevertheless, not all studies are consistent with these findings, since Percival et al. [52] on Aesculus hippocasta-num L. and Quercus robur L showed no significant effects of film-forming polymers on leaf F v /F m values and SPAD index values compared with C.
However, our findings are confirmed by other studies where the SPAD index reading was greater in leaves treated with kaolin [51]; this positive effect on SPAD index was also shown by Lombardini et al. [25] after kaolin treatment.
A possible explanation for the increase of this parameter may be due to the fact that leaves not treated with kaolin can show a lower light reflectance, suggesting an increase of degradation of the photosynthetic pigments [53].
In September, at the end of the test, the plants were sampled to determine the dry matter ( Figure 4). An increase in both roots and total dry matter with V application compared to C was highlighted, equal to +17.43% and +12.07%, respectively, while K applications caused a reduction in total dry matter compared to C, equal to −23.63%. Our findings concerning K application are consistent with those reported previously by Cantore et al. [24], where the use of kaolin did not affect fruit dry matter. Similar results have also been observed in other previous studies on anti-transpirant products that influenced plant height and total dry matter [42], where the results obtained in greenhouse and field trials on unirrigated sweet corn indicated that significant increases in growth and of dry matter/yield occur in response to Vapor Gard (6%) treatment. Positive effect of 1-p-menthene-based product on vegetative growth and development may be due to improved plant water status related to lower transpiration [54].   Table 2 reports the phenolic composition obtained by UHPLC-HRMS analysis and Table 3 shows the quali-quantitative polyphenolic profile of olive leaves in control and treated plants. Single phenolic compounds were identified and quantified using calibration curves built with appropriate reference compounds. As certain standards were not available (the case of secoiridoids), quantification was calculated employing calibration curves of oleuropein, while for the identification, MS / MS experiments had to be used.

Analysis of Polyphenols by UHPLC-Q-Orbitrap HRMS
The Q Exactive Orbitrap LC-MS/MS analysis allowed the separation and identification of six phenolic compounds, already reported in olive leaves. They were identified by  Table 2 reports the phenolic composition obtained by UHPLC-HRMS analysis and Table 3 shows the quali-quantitative polyphenolic profile of olive leaves in control and treated plants. Single phenolic compounds were identified and quantified using calibration curves built with appropriate reference compounds. As certain standards were not available (the case of secoiridoids), quantification was calculated employing calibration curves of oleuropein, while for the identification, MS/MS experiments had to be used.  Table 3. Effect of kaolin (K) and 1-p-menthene (V) on phenolic compounds content in leaves of olive trees at the end of growing season. C: control. OH: hydroxy. The Q Exactive Orbitrap LC-MS/MS analysis allowed the separation and identification of six phenolic compounds, already reported in olive leaves. They were identified by comparison of their molecular formula, the fragments obtained, and the order of elution in the literature [32]. The chromatographic separation and qualitative analysis highlighted a nonsubstantial difference in the phenolic composition between the different samples investigated. The two main phenolic compounds detected were oleuropein and tyrosol glucoside in all three treatments. K treatments showed significantly higher values of hydroxytyrosol glucoside with an increase of +276.00% compared to C. On the contrary, V application caused significantly lower values than that of C (−55.11%). This trend is also confirmed for oleuropein, where the highest concentration is shown with K application, which showed an increase compared to C of +196.94%; on the contrary, V application did not show significant differences compared to C. K application also positively influenced the total polyphenols content; an increase of +240.33% (11,180.05 µg g −1 ) was recorded with respect to C (3285.04 µg g −1 ), while V treatment induced the lowest accumulation of polyphenols content (1893.07 µg g −1 . Treatment with pinolene caused an inhibitory effect on PAL activity, thus reducing the concentration of polyphenolic compounds. In literature, a similar effect was associated with the foliar application of the antioxidant 5-hydroxybenzi midazole on "Koroneiki" olive trees [55].

Antioxidant Activity of Polyphenolics Extracts
The results of the antioxidant activity assays, conducted on the polyphenolics extracts of the olive leaves, were reported in Figure 5 and expressed as TEAC (mmol Trolox kg −1 dw). According to ABTS test, the values obtained showed an improvement of antioxidant activity with K treatment (+49.09%) and a reduction with V treatment (36.16%) with respect to C. As for the DPPH test, the obtained values showed an improvement of antioxidant activity only with K treatments, with an increase of +39.47% with respect to C. Our results are in agreement with Denaxa et al. [55], who reported that kaolin clay particles resulted in the highest total phenol concentration in young olive plants. In this same study, it was highlighted that Kaolin application resulted in high activities of antioxidant enzymes such as superoxide dismutase, peroxidase and glutathione reductase under water deficit compared to other alleviating products in investigation. Brillante et al. [56] reported that pinolene treatment on grapes caused a decrease in sugar content and anthocyanin level compared to control. In addition, recent studies on Vitis Vinifera showed that treatments with kaolin have highlighted positive effects on reducing canopy temperatures, enhancing the accumulation of anthocyanins [57].
with respect to C. As for the DPPH test, the obtained values showed an improvement of antioxidant activity only with K treatments, with an increase of +39.47 % with respect to C. Our results are in agreement with Denaxa et al. [55], who reported that kaolin clay particles resulted in the highest total phenol concentration in young olive plants. In this same study, it was highlighted that Kaolin application resulted in high activities of antioxidant enzymes such as superoxide dismutase, peroxidase and glutathione reductase under water deficit compared to other alleviating products in investigation. Brillante et al. [56] reported that pinolene treatment on grapes caused a decrease in sugar content and anthocyanin level compared to control. In addition, recent studies on Vitis Vinifera showed that treatments with kaolin have highlighted positive effects on reducing canopy temperatures, enhancing the accumulation of anthocyanins [57].

Principal Component Analysis (PCA)
A principal component analysis (PCA) was conducted to highlight the effects of the treatments on all the parameters analyzed above. The first two principal components (PCs) explained 100% of the cumulative variance ( Figure 6), with PC1 accounting for 76.71% and PC2 for 23.29%. The PC1 was positively correlated with the nutraceutical compounds: OH-tyrosol glucoside, verbascoside, oleuropein, ligstroside, luteolin, oleuropein aglycone, total polyphenols and with antioxidant activity assays (ABTS and DPPH), and negatively correlated with the stomatal conductance, fluorescence (F v /F m ), TSCA and dry matter of roots, leaves and total. Moreover, PC2 was positively correlated with shoots growth, leaf area, RWC and SPAD index.
PCA is effective in plotting the physiological, biometric, and nutraceutical parameters of the young olive trees in relation to the different treatments and their usefulness. In particular, the Kaolin (K) treatment was positioned in the upper right quadrant of the PCA score plot, as it delivered the highest value of total polyphenols and all single polyphenols analyzed except for luteolin and verbascoside, and it showed the highest value of DPPH and SPAD. The pinolene (V) treatment was positioned in the upper left quadrant of PCA score, as it delivered the highest value of RWC, F v /F m , shoots growth, TCSA and dry matter of roots, wood and total dry matter, whereas control was located in the lower left quadrant.
PCA score plot, as it delivered the highest value of total polyphenols and all single polyphenols analyzed except for luteolin and verbascoside, and it showed the highest value of DPPH and SPAD. The pinolene (V) treatment was positioned in the upper left quadrant of PCA score, as it delivered the highest value of RWC, Fv/Fm, shoots growth, TCSA and dry matter of roots, wood and total dry matter, whereas control was located in the lower left quadrant. Figure 6. Principal component analysis (PCA) of biometric parameters (shoots growth, TCSA (trunk cross-sectional area), leaf area and dry matter of roots, wood, leaves and total dry matter), physiological parameters (stomatal conductance, RWC, SPAD and Fv/Fm), nutraceutical parameters (OH-tyrosol glucoside, verbascoside, oleuropein, ligstroside, luteolin, oleuropein aglycone, total polyphenols, ABTS and DPPH), under the application of two anti-transpirants K: kaoline and V: di-1-p-menthene in comparison to C: control. For the PCA, the parameters of stomatal conductance, RWC and SPAD analyzed were those of August, and for Fv/Fm those of 2:00 pm (the values obtained after prolonged thermal stress were considered).

Conclusions
This preliminary study emphasizes the importance of using products based on kaolin and pinolene to improve the biometric, physiological and nutraceutical characteristics of young olive trees subjected to high thermal stress. As tentative results, foliar application of the anti-transpirant pinolene-based product improved the biometric parameters of the plants, in particular the shoots growth, TCSA, and total dry matter. Plants sprayed with the tested preparation were characterized by a higher relative water content (RWC) Figure 6. Principal component analysis (PCA) of biometric parameters (shoots growth, TCSA (trunk cross-sectional area), leaf area and dry matter of roots, wood, leaves and total dry matter), physiological parameters (stomatal conductance, RWC, SPAD and F v /F m ), nutraceutical parameters (OH-tyrosol glucoside, verbascoside, oleuropein, ligstroside, luteolin, oleuropein aglycone, total polyphenols, ABTS and DPPH), under the application of two anti-transpirants K: kaoline and V: di-1p-menthene in comparison to C: control. For the PCA, the parameters of stomatal conductance, RWC and SPAD analyzed were those of August, and for F v /F m those of 2:00 p.m. (the values obtained after prolonged thermal stress were considered).

Conclusions
This preliminary study emphasizes the importance of using products based on kaolin and pinolene to improve the biometric, physiological and nutraceutical characteristics of young olive trees subjected to high thermal stress. As tentative results, foliar application of the anti-transpirant pinolene-based product improved the biometric parameters of the plants, in particular the shoots growth, TCSA, and total dry matter. Plants sprayed with the tested preparation were characterized by a higher relative water content (RWC) in leaves and a higher value of efficiency of photosystem II (PSII); this positive effect of 1-p-menthene on vegetative growth and development may be due to improved plant water status related to lower transpiration. Kaolin application also reduced stomatal conductance and similar to the V application, recorded higher values of leaf chlorophyll concentrations (SPAD) at the end of the vegetative season. In particular, interesting effects were recorded with the use of kaolin on the nutraceutical compounds of olive leaves; high contents of total polyphenols were recorded compared to control plants and this product showed a higher value of the antioxidant activity.
It is important to underline that further studies are necessary to determine the effect of these products on the biometric, physiological and nutraceutical parameters of mature olive trees cultivated in open field conditions in consecutive years and on the quality of the produce.