Lime and Organic Manure Amendment Enhances Crop Productivity of Wheat–Mungbean–T. Aman Cropping Pattern in Acidic Piedmont Soils

: Soil acidity is a major problem when it comes to improving crop productivity and nutrient uptake. This experiment was therefore conducted at a farmer’s ﬁeld—Nalitabari Upazila under AEZ 22 (northern and eastern Piedmont plains) to evaluate the effects of lime and organic manure (OM) amendment on crop productivity and nutrient uptake of the wheat–mungbean–T. Aman cropping pattern in acidic soils of northern and eastern Piedmont plains. The experiment was laid out in a randomized complete block design with three replications. There were nine treatments applied, varying doses of lime (dololime at the rate of 1 and 2 t ha − 1 ), OM (cow dung at the rate of 5 t ha − 1 , poultry manure at the rate of 3 t ha − 1 ) and a lime–OM combination to the ﬁrst crop; T. Aman and its residual effects were evaluated in the succeeding second crop, wheat, and the third crop, mungbean. Results demonstrate that application of lime and organic manure to soil had signiﬁcant effects on the ﬁrst crop. However, the effects of lime and organic manure were more pronounced in the second and third crops. The increase in grain yield over control ranged from 0.24 to 13.44% in BINA dhan7. However, it varied from 10.14 to 54.38% in BARI Gom30 and 40 to 161.67% in BARI Mung6. The straw yields of the crops also followed a similar trend. The N, P, K, and S uptake by grain and straw of T. Aman, wheat, and mungbean were inﬂuenced signiﬁcantly by the combined application of lime and organic manure. Sole or combined application of lime and manure amendment signiﬁcantly improved nutrient availability and soil quality. Therefore, application of lime in combination with manure can be practiced for uplifting the crop productivity and improving soil quality in acidic Piedmont soils of northern and eastern Piedmont plains.


Introduction
Acidic soils have toxic concentrations of A1 3+ , Fe 3+ , and Mn 2+ , lower concentrations of P, and low availability of bases, which cause reduction in crop yield [1][2][3]. Legumes are highly affected due to soil acidity. Acidity limits the survival and persistence of nodule bacteria in soil, and the process of nodulation is hampered. Acidic soils (pH < 5.5) affect plant growth directly or indirectly by influencing the availability of plant nutrients, particularly phosphorus, secondary nutrients (Ca, Mg), and micronutrients (Mo, B, and Zn), reducing microbial activity, and creating toxicity of Fe and Mn (Al in some cases) [4]. Soil acidification may intensify and affect crop production if effective management strategies for amelioration are not implemented [5].
of Nalitabari Upazila in the northern and eastern Piedmont plains. This research will aid farmers in increasing crop yields in acid-prone locations.

Experimental Site and Soil Properties
The experiment was carried out at the farmer's field of Ramchandrakura Union, Nalitabari Upazila, Sherpur (25 • 11 N, 90 • 15 E) from July 2017 to May 2018. The experimental site belongs to the agroecological zone, northern and eastern Piedmont plains . According to general soil type classification, the site falls under grey terrace soil [21,22]. Topographically, the experimental site was medium-high to high. Before starting the experiment, 20 initial composite soil samples (0-15 cm depth) were collected from the experimental plots and analyzed using standard methods. The soil was sandy loam in texture and strongly acidic in nature, with a pH of 4.66, organic C 0.89%, total N 0.12%, available P 6.99 ppm, exchangeable K 22.94 ppm, and available S 1.67 ppm.

Preparation of Experimental Plots and Growing Crops
With a power tiller, the land was prepped by ploughing and cross ploughing. The soil was then laddered using traditional methods. Before final land preparation and laying out, all weeds and stubbles were removed from the field. To limit the heterogenic effects of soil, the experiment was set up in a randomized complete block design (RCBD), with the experimental area separated into three blocks representing the replications. Each block was subdivided into nine plots and the treatments were randomly distributed to the unit plots in each block. Thus, the total number of unit plots was 27. Each plot was 4 × 2.5 m in size and was separated from the others by ails (30 cm). There was a 1 m drain between the blocks (i.e., it separated the blocks from each other). The fertilizers were applied as per treatments following the Bangladesh Agricultural Research Council (BARC) [23] Fertilizer Recommendation Guide (2012). The recommended doses (RDs) of chemical fertilizers were 90 kg N, 10 kg P, 35 kg K, 8 kg S and 1 kg B per ha for T. Aman rice; 120 kg N, 30 kg P, 60 kg K, 15 kg S, and 1.3 kg Zn for wheat; and 18 kg N, 18 kg P, 24 kg K, 12 kg S, 2 kg Zn, and 1.2 kg B for mungbean. The sources of N, P, K, S, and Zn were urea, triple super phosphate, muriate of potash, gypsum, and zinc sulfate, respectively. The full doses of chemical fertilizers were applied in all the treatments, including the control. All of the chemical fertilizers, except urea, were applied during final land preparation. The urea was applied in three equal splits for T. Aman rice and two equal splits for wheat and mungbean, respectively. Dololime, cow dung, and poultry manure were applied before 2 weeks of planting crops and mixed with soil. Lime, cow dung, and poultry manure were applied to the first crop and their residual effects were evaluated in the succeeding second and third crops. Seedlings were grown in a nursery bed and 30-35-day old seedlings were carefully uprooted and transplanted in the plots, maintaining a spacing of 20 × 20 cm in the case of T. Aman rice. Three seedlings were transplanted at each hill. Wheat and mungbean seeds were sown after final land preparation. Intercultural operations, such as irrigation, weeding, and pest control were done when necessary, to ensure and maintain a favorable environment for normal growth and development of the crops.

Harvesting and Data Recording
The crops were harvested at maturity. An area of 1 m 2 was harvested from each plot and the harvested crop was bundled separately. Then the bundles were brought to the threshing floor and threshed. The grain yield was obtained on a 14% moisture basis while the straw yield was recorded on a sun-dry basis. Grain and straw samples were analyzed for total nitrogen concentration following the semi-micro Kjeldahl method [24], phosphorus was determined by the Olsen method [25], potassium was determined by the flame photometer method, and sulfur was determined by the spectrophotometer method.
The N, P, K, S uptake by grain and straw was determined from grain and straw yield data. The nutrient uptake was determined by formula [26]: Nutrient uptake = Nutrient content (%) × Dry mass production (kg/ha) 100

Analysis of Soil Samples before and after the Experiment
The initial and post-harvest soil samples were used to determine the properties of the soil, including soil organic matter (SOM) content, soil total nitrogen (STN), available P, exchangeable Ca and Mg, pH, electrical conductivity (EC), and cation exchange capacity (CEC). SOM content was calculated by multiplying organic carbon (OC) by 1.73, as suggested by Ghosh et al. [27] and OC was determined titrimetrically following the Walkley and Black method [28]. STN was determined by the semi-micro Kjeldahl method [24]; available P was extracted from the soil by shaking with 0.03 M NH4F-0.025 M HCl solution at pH < 7.0 following the Bray and Kurtz method [29]. The exchangeable calcium (Ca) and magnesium (Mg) contents were extracted by the ammonium acetate extraction method and determined by ethylene-di-amine tetra acetic [30]. The pH of the samples was assessed in a soil: water ratio of 1:2.5 with a glass electrode pH meter [30]. The EC of collected soil samples was determined electrometrically (1:5 = soil:water ratio) by a conductivity meter using 0.01 M KCl solution to calibrate the meter following the procedure described by Ghosh et al. [27]. CEC was determined by the NH 4 OAc extraction method, as suggested by Chapman [31].

Statistical Analysis
The data were analyzed statistically by the F-test and the mean differences were adjudged by Duncan's new multiple range test (DMRT), as outlined by Gomez and Gomez [32]. The grain yield of first crop (Binadhan 7) responded significantly to the application of dololime, cow dung, and poultry manure, although there was a little difference in grain yield among the treatments (p < 0.05) ( Table 2). The grain yield ranged from 4.24 to 4.81 t ha −1 . The highest grain yield (4.81 t ha −1 ) was observed in T 7 (Lime-1 OM-2, dololime 1 t ha −1 , poultry manure 3 t ha −1 ) and the lowest value (4.24 t ha −1 ) was recorded in T 1 (control). Based on grain yield, the treatments may be ranked in order of T 7 > T 8 > T 5 > T 9 > T 4 > T 3 > T 2 > T 6 = T 1 . The increase in grain yield over the control ranged from 0.24 to 13.44% where the highest increase was obtained in T 7 (13.44%) and the lowest one was obtained in T 6 (0.24%). Table 2. Effect of lime and manure amendment on grain and straw yield (mean ± SE; n = 3) of wheat-mungbean-T. Aman cropping pattern. Straw yield of Binadhan 7 was also significantly influenced by different treatments under study. The yields of straw ranged from 4.51 to 4.98 t ha −1 (p < 0.05) ( Table 2). The highest straw yield of 4.98 t ha −1 was obtained in T 8 (Lime-2 OM-1, dololime 2 t ha −1 , cow dung 5 t ha −1 ) and the lowest value of 4.51 t ha −1 was noted in T 1 (control). The treatment may be ranked in the order of T 8 > T 7 >T 5 > T 4 > T 9 > T 2 > T 3 > T 6 > T 1 in terms of straw yield. Regarding the % increase of straw yield, maximum increase (10.42%) was noted in T 8 and the minimum one (1.55%) was found in T 6 .

Grain and Straw Yield of Wheat
The grain yield of BARI Gom30 responded significantly to the residual dololime, cow dung and poultry manure ( Table 2). The grain yield ranged from 2.17 to 3.35 t ha −1 . The highest grain yield (3.35 t ha −1 ) was observed in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ), which was statistically similar to T 8 (Lime-2 OM-1, dololime 2 t ha −1 , cow dung 5 t ha −1 ), and T 7 (Lime-1 OM-2, dololime 1 t ha −1 , poultry manure 3 t ha −1 ) whereas the lowest value (2.17 t ha −1 ) was recorded in T 1 (control). The increase in grain yield over control ranged from 10.14 to 54.38% where the highest increase was obtained in T 9 and the lowest one was obtained with T 4 . Based on grain yield, the treatments may be ranked in order of T 9 > T 8 > T 7 > T 6 > T 3 > T 2 > T 5 > T 4 > T 1 .
Straw yields of BARI Gom30 also responded significantly to the different treatments under study. The yield of straw ranged from 3.11 to 4.79 t ha −1 ( Table 2). The highest straw yield of 4.79 t ha −1 was obtained in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and the lowest value of 3.11 t ha −1 was noted in T 1 (control). The treatments may be ranked in the order of T 9 > T 8 > T 7 > T 6 > T 3 > T 2 > T 5 > T 4 > T 1 in terms of straw yield. Regarding the percent increase of straw yield, maximum increase (54.02%) was noted in T 9 and the minimum one (9.65%) was found in T 4 .

Grain and Straw Yield of Mungbean
Residual effect of lime (dololime) and organic manure (cow dung and poultry manure) showed a significant influence on seed yield of BARI Mung6 (Table 2) (p < 0.05). The seed yield ranged from 0.60 t ha −1 to 1.57 t ha −1 . The highest seed yield (1.57 t ha −1 ) was found in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ), which was statistically similar with T 7 (Lime-1 OM-2, dololime 1 t ha −1 , poultry manure 3 t ha −1 ) and T 8 (Lime-2 OM-1, dololime 2 t ha −1 , cow dung 5 t ha −1 ). The lowest value (0.60 t ha −1 ) was observed in T 1 (control). The increase in seed yield over control ranged from 40 to 161.67%, where the highest increase was obtained in T 9 and the lowest one was obtained with T 4 ( Table 2). The results demonstrated that the application of dololime alone or combination with cow dung or poultry manure increased the seed yield of mungbean to a significant extent. The seed yields obtained from different treatments may be ranked in order of T 9 > T 8 > T 7 > T 6 > T 3 > T 2 > T 5 > T 4 > T 1 .
Straw yield of BARI Mung6 was also significantly influenced by different treatments under study. The yields of straw ranged from 1.15 to 3.33 t ha −1 (p < 0.05) ( Table 2). The highest straw yield of 3.33 t ha −1 was obtained in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and the lowest value of 1.15 t ha −1 was noted in T 1 (control). The treatment may be ranked in the order of T 9 > T 8 = T 7 > T 6 > T 3 = T 2 > T 5 = T 4 > T 1 in terms of straw yield. Regarding the % increase of straw yield, maximum increase (189.57%) was noted in T 9 and the minimum one (40.87%) was found in T 4 .

Effect of Lime and Organic Manure Amendment on Nutrient Uptake of Wheat-Mungbean-T. Aman Cropping Pattern
Nutrient contents of grain and straw of all three crops were determined as depicted in Supplementary Table S1, and nutrient uptake by crops was calculated. Nutrient uptake by the wheat-mungbean-T. Aman cropping pattern is described as follows.

Nutrient Uptake by T. Aman
The overall uptake of the macronutrients N, P, K, and S by T. Aman was significantly impacted by the use of lime and organic amendments (p < 0.05). The N uptake by grain and straw of T. Aman rice due to application of lime and organic amendment ranged from 42.78 to 58.21 kg ha −1 and 13.97 to 19.74 kg ha −1 (Table 3). Experimental plots that had T 8 (Lime-2 OM-1, dololime 2 t ha −1 , cow dung 5 t ha −1 ) treatment took up the highest total N, which was statistically similar to T 7 and T 9 in case of grain and T 7 in case of straw, whereas the lowest value was observed in T 1 (control) in both cases ( Table 3). The total P uptake ranged from 8.87 to 11.94 kg ha −1 and 5.39 to 10.06 kg ha −1 in grain and straw, respectively. The highest total P uptake was observed in T 7 (Lime-1 OM-2, dololime 1 t ha −1 , poultry manure 3 t ha −1 ) and the lowest value in T 1 (control) ( Table 3). The value of K uptake by T. Aman ranged from 11.01 to 16.92 kg ha −1 and 50.43, and 61.97 kg ha −1 in grain and straw, respectively. The highest K uptake was recorded in T 8 (Lime-2 OM-1, dololime 2 t ha −1 , cow dung 5 t ha −1 ), which was similar to T 7 and T 9 , and the lowest value was observed in T 1 (Table 3). Likewise, S uptake by grain and straw ranged from 11.44 to 16.53 kg ha −1 and 8.11 to 13.08 kg ha −1 , respectively (Table 3). Table 3. Nutrient uptake (mean ± SE; n = 3) by crops in wheat-mungbean-T. Aman cropping pattern as influenced by lime and manure amendment.

Nutrient Uptake by Wheat
In the second crop of the cropping pattern, the residual effect of lime and organic manure application on the nutrient uptake was more prominent. The residual effect of lime and organic manure influenced the uptake of N, P, K, and S by grain and straw of wheat significantly (p < 0.05). Total N uptake by wheat ranged from 36.85 to 63.10 kg ha −1 and 10.25 to 21.05 kg ha −1 in grain and straw, respectively. The T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) had the highest N uptake by the grain and straw and T 1 (control) had the lowest (Table 3). Similarly, T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) had the highest P, K, and S uptake while T 1 (control) had the lowest ( Table 3). The uptake of P, K, and S ranged from 4.55 to 12.00 kg ha −1 , 8.68 to 18.37 kg ha −1 and 4.29 to 9.36 kg ha −1 in grain, and 2.08 to 3.93 kg ha −1 , 44.17 to 73.71 kg ha −1 and 4.62 to 10.07 kg ha −1 in straw, respectively (Table 3).

Nutrient Uptake by Mungbean
N, P, K, and S uptake by grain and straw of mungbean was significantly influenced by the residual effect of lime and organic manure application (p < 0.05). The N uptake by mungbean grain and straw ranged from 21.77 to 61.67 kg ha −1 and 5.54 to 6.84 kg ha −1 , respectively. The highest N uptake by grain and straw was observed in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and the lowest value was observed in T 1 (control) ( Table 3). Likewise, the highest P, K, and S uptake by grain and straw were recorded in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and the lowest value was observed in T 1 (control) ( Table 3). The uptake of P, K, and S ranged from 2.40 to 9.60, 8.52 to 25.02 and 2.40 to 8.81 kg ha −1 in grain, and 0.48 to 0.96, 3.56 to 4.04 and 0.32 to 0.51 kg ha −1 in straw, respectively (Table 3).

Effect of Lime and Manure Amendment on Soil Properties under Wheat-Mungbean-T. Aman Cropping Pattern
Application of lime and manure significantly improved soil fertility and soil properties ( Table 4). SOM content ranged from 1.54 to 1.63% and 1.31 to 1.56% before and after the experiment, respectively, exhibiting the highest value in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ), which was statistically similar to T 7 (Lime-1 OM-2, dololime 1 t ha −1 , poultry manure 3 t ha −1 ) and the lowest value in T 1 (control) ( Table 4). Similarly, STN varied from 0.11 to 0.16% and 0.09 to 0.19% before and after the experiment, respectively (Table 4). STN decreased about 5-20% after the experiment where there was sole application of chemical fertilizer and lime along with chemical fertilizer, whereas STN increased 1 to 15% due to sole application of manure amendment or combined application of lime and manure amendment, along with chemical fertilizers (Table 4). Available P content in soil was 7.05 to 7.96 ppm before the experiment and 6.77 to 11.54 ppm after the experiment. Available P content after the experiment decreased about 4% in the control, whereas sole or combined application of lime and manure amendment increased available P content about 15 to 45% compared to the initial state. The highest increase was observed in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and the lowest one in T 4 (OM-1, cow dung 5 t ha −1 ) ( Table 4). Exchangeable Ca content in soil ranged from 5.20 to 5.55 cmol c /kg and 4.68 to 6.88 cmol c /kg, respectively, before and after the experiment ( Table 4). The increase in exchangeable Ca content in soil ranged from −10 to 24% after the experiment compared to their pre-experiment condition exhibiting the highest increase in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and a decrease in T 1 (control) ( Table 4).
Exchangeable Mg content ranged from 1.35 to 1.72 cmol c /kg and 1.22 to 2.11 cmol c /kg before and after the experiment, respectively, exhibiting the highest value in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and the lowest value in T 1 (control) ( Table 4). The change in exchangeable Mg content in soil ranged from −10 to 23% after the experiment (Table 4). Likewise, pH of the soil varied from 4.66 to 5.34 and 4.10 to 6.69 before and after the experiment, respectively (Table 4). Soil pH increased about −12 to 26% after the experiment due to application of lime and manure amendment along with chemical fertilizers (Table 4). Soil pH decreased when no lime or manure amendment was applied whereas the highest increase was observed in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ). EC of the soil was 0.22 to 0.38 dS/m before the experiment and 0.22 to 0.47 dS/m after the experiment. EC of the soil after the experiment increased 2 to 25% compared to the initial state due to application of lime and manure amendment ( Table 4). The highest increase was observed in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and the lowest in T 1 (control) ( Table 4). CEC of the soil ranged from 34.97 to 38.22 cmol c /kg and 33.14 to 45.86 cmol c /kg, respectively, before and after the experiment ( Table 4). The increase in exchangeable Ca content in soil ranged from −6 to 20% after the experiment compared to their pre-experiment condition exhibiting the highest increase in T 9 (Lime-2 OM-2, dololime 2 t ha −1 , poultry manure 3 t ha −1 ) and a decrease in T 1 (control) ( Table 4).

Correlation among Soil Properties, and between pH and Crop Yield in Wheat-Mungbean-T. Aman Cropping Pattern
Pearson's correlation matrix among the soil properties is shown in Table 5. The results revealed strong significant positive correlation among the soil properties, i.e., soil fertility indices. With an increase in SOM content (%), soil macronutrients and soil reaction increased and improved other chemical properties (Table 5). Soil reaction had significant positive effects on nutrient availability and improvement of soil quality. With the increase of soil pH, availability of primary and secondary macronutrients increased, and consequently the EC and CEC of the soil improved (Table 5). A synergistic effect was observed among the plant nutrients (i.e., N, P, Ca, and Mg) ( Table 5). Availability of exchangeable Ca and Mg was boosted up due to increase in soil EC and CEC.  Soil pH had significant but variable effects on grain and straw yield of all three crops (Figure 1). In regard to T. Aman rice, the response of straw yield (R 2 = 0.49) was higher than that of grain yield (R 2 = 0.43) (Figure 1). The effect of soil pH on the grain and straw yield was more pronounced in wheat than that in T. Aman rice (Figure 1). The response of straw yield (R 2 = 0.77) was greater than grain yield (R 2 = 0.62) (Figure 1). The grain and straw yield of mungbean showed the highest responses to the soil pH (Figure 1). Straw yield (R 2 = 0.80) showed higher response than grain yield (R 2 = 0.78) (Figure 1).

Discussion
Soil acidity is a restriction of crop productivity in 30% of the earth surface an of agricultural land all over the world, including Bangladesh [33][34][35]. Soil acidificati be caused by a variety of sources, including natural processes, industrial pollutan agricultural output [36]. Acidified soil diminishes the availability of many essentia ents, and worsens the toxicity of others (e.g., Al 3+ , Fe 2+ ) via modifying a variety of ch and biological processes in the soil [35].
Liming is one of the most essential and successful management strategies for ing soil acidity [37]. The influence of liming on soil pH is highly dependent on the q of the liming materials, e.g., particle size and material kinds [38], as well as the p original chemical and physical qualities, e.g., pH buffering capacity and soil organ ter [39]. Moreover, high SOM frequently results in high soil CEC and pH buffer ca [40]; hence, the impacts of lime tended to be stronger when the original soil SO high. This is due to the intricate interactions between soil, plants, and the environm well as the variability of the physical and chemical features of soil [37]. Our results s that the application of lime and organic amendment increased the pH about 26% af experiment, whereas chemical fertilizer increased soil acidity by 4% (Table 4)

Discussion
Soil acidity is a restriction of crop productivity in 30% of the earth surface and 57% of agricultural land all over the world, including Bangladesh [33][34][35]. Soil acidification can be caused by a variety of sources, including natural processes, industrial pollutants, and agricultural output [36]. Acidified soil diminishes the availability of many essential nutrients, and worsens the toxicity of others (e.g., Al 3+ , Fe 2+ ) via modifying a variety of chemical and biological processes in the soil [35].
Liming is one of the most essential and successful management strategies for reducing soil acidity [37]. The influence of liming on soil pH is highly dependent on the quality of the liming materials, e.g., particle size and material kinds [38], as well as the primary original chemical and physical qualities, e.g., pH buffering capacity and soil organic matter [39]. Moreover, high SOM frequently results in high soil CEC and pH buffer capacity [40]; hence, the impacts of lime tended to be stronger when the original soil SOM was high. This is due to the intricate interactions between soil, plants, and the environment, as well as the variability of the physical and chemical features of soil [37]. Our results showed that the application of lime and organic amendment increased the pH about 26% after the experiment, whereas chemical fertilizer increased soil acidity by 4% (Table 4), which is in line with Ozlu and Kumar [41]. Any of the following processes or combinations might have caused the rise in soil pH with the addition of lime and manure: consumption of proton by functional groups linked to organic materials [42], decarboxylation of organic acid anions during decomposition, specific adsorption of organic molecules by ligand exchange with the release of OH [43], and the release of OH ions during reduction reactions associated with localized anaerobic microsites (these are all examples of proton consumption) [44]. STN and available P content in soil increased due to increase in soil pH as a result of lime and manure application, which was reported earlier by Van Chuong [45]. The availability of exchangeable Ca 2+ and Mg 2+ increased as soil pH increased due to the application of lime and organic amendment, which was also observed by Mosharrof et al. [46] and Kunhikrishnan et al. [7]. Increased soil pH as a result of lime and manure amendment also increased soil EC and CEC, which was consistent with Yagi et al. [47] and Kisić et al. [48].
Liming can also influence both transformation and uptake of nutrients by plants [49,50] and, additionally, nutrient use efficiency [34]. It is widely accepted that liming can neutralize excessive acidic ions in the soil including proton ions and other acidic mineral cations (e.g., Al 3+ ), while simultaneously supplying basic cations to the root zones e.g., Ca 2+ and Mg 2+ [51]. Liming can improve soil chemical properties, such as CEC and pH, and enhance the availability and uptake of macronutrients (e.g., P and K) [52]. Liming increases crop production primarily through direct effects on improving soil physical, chemical, and biological characteristics, which lead to increased availability and mobility of many essential nutrients of plants [39,53]. Liming has an active and passive positive influence on soil pH, plant nutrient mobilization, soil aggregates and structure, and soil biological activity [39,54]. Lime treatment in acid soils improves nutrient (NPK) availability and creates a healthier environment in the rhizosphere zone of the plants when the soil pH is low or the acidity is high [55][56][57]. Kemmitt et al. [58] found that changes in soil pH had a substantial impact on the pace of soil C and N cycling, and that liming treatments improved soil microbial activity by elevating the pH. Soil pH and P availability have a considerable relationship [59], which determines how P nutrition for arable crops is optimized. According to Barrow [9], the optimal soil pH for P absorption has to be re-evaluated. However, pH is not the only factor to consider; organic matter content also influences yield response to P [60]. In Ethiopia, Alemu et al. [61] and in Germany, von Tucher et al. [62], the impact of P status on yield response to pH was recently revealed for barley. Our result demonstrated that both grain and straw yield of T. Aman, wheat, and mungbean were strongly correlated with soil pH (Figure 1). Grain and straw yields also had strong positive correlation with other physicochemical properties of soil, as all the soil properties were positively correlated with soil pH (Table 5).
Lime and organic manure amendment have variable effects, i.e., increase in yield and nutrient uptake over control on three different crops of this experiment. In case of the first crop, T. Aman (Binadhan 7) rice, the effect of lime and organic manure was less prominent. However, a significant effect of lime or organic manure alone or in combination was observed in wheat (BARI Gom30) and mungbean (BRRI Mung6). It is particularly noticeable in yields of wheat and mungbean. In the instance of Binadhan 7, the increase in grain production over control varied from 0.24 to 13.44%. Rice yields have also been reported to rise as a result of liming [63,64]. The straw yields of the crops also followed the similar trend. Liming raises the pH of the soil and lowers the acidity, resulting in higher straw yields [65][66][67].
Our results showed that residual effect of lime and organic manure significantly increased yield (Table 2), nutrient content (Table S1), and nutrient uptake (Table 3) of wheat, which was reported earlier by Sultana et al. [68]. The increase in grain yield over control ranged from 10.14 to 54.38%. Caires et al. [63] observed that surface liming caused increases up to 140% in the grain yield of wheat. In another experiment, Arsat et al. [69] reported that combined application of 5 t manure and 2.2 t ha −1 lime increased grain and straw yield by 279% and 187%, respectively, over the control. Kisić et al. [47] discovered that, in addition to mineral and organic fertilization, liming resulted in considerably greater yields than the control and considerably higher yields than mineral fertilizer treatments. According to Jovanovic et al. [70], liming had a significant impact on field crop yields, and using high rates in a single application was preferable to using low rates repeatedly. Similar results were also reported by Samia [71] and Basak [72]. According to Von Tucher et al. [62], Liming in low pH soils with barley (and wheat) boosts fertilizer usage efficiency, The residual effect of lime and organic manure application was more pronounced in the third crop, mungbean. Our results demonstrated that residual effect of combined application of lime and organic manure significantly increased seed yield (Table 2), and nutrient content and uptake (Table 3) of mungbean, which is in line with previous experimental results [8,[73][74][75]. Kasa et al. [76] found that application of different level of lime and phosphorus significantly increased the yield and yield contributing characters of Haricot bean in Ethiopia. Lime in an acid soil not only replaces hydrogen ions, elevates soil pH [8], and increases NPK availability, but it also promotes plant growth and development in legume crops [77]. In an acidic soil, an experiment was carried out on Sesamum, mungbean, and cowpea with lime applications of 0.5, 1.0, 1.5, and 2.0 tons per hectare, respectively. The greatest rate of lime treatment favored lowering soil acidity, increasing NPK availability, neutralizing soil pH, and increasing yield [57].
The combined application of lime and organic amendment greatly boosted the total production and nutrient uptake of each crop in the T. Aman-mustard-boro cropping pattern, according to our findings. Sultana et al. [20] found that applying 1 t ha −1 dololime coupled with 3 t ha −1 poultry manure or 5 t ha −1 FYM boosted crop output and nutrient uptake in the old Himalayan Piedmont plain's potato-mungbean-rice cropping pattern.

Conclusions
The overall results of the study demonstrate that addition of lime and/or organic manure to acid soils significantly increased yield and nutrient uptake of the crops of the wheat-mungbean-T. Aman cropping pattern. Lime or organic manure alone improved crop yield to a significant extent. However, combined application of lime and organic manure remarkably increased the yield and nutrient uptake of the crops as well as improved the nutrient availability and other soil properties. Based on the findings, it can be concluded that combined application of dololime and manure amendment (poultry manure or cow dung) can be practiced for better crop productivity of wheat-mungbean-T. Aman cropping pattern and improvement of soil quality in acidic Piedmont soils. However, similar research in other acid-prone areas of Bangladesh, on the other hand, would be worth considering for broader recommendations.

Data Availability Statement:
The data that support this study will be shared upon reasonable request to the corresponding author.