Exploring Substrate Water Capture in Common Greenhouse Substrates through Preconditioning and Irrigation Pulsing Techniques

: Particles in a substrate create a network of pore pathways for water to move through, with size and shape determining the efﬁcacy of these channels. Reduced particle size diversity can lead to increased leachate, poor substrate hydration, and an inefﬁcient irrigation practice. This research examined the hydration characteristics of three greenhouse substrate components at three preconditioned initial moisture contents using subirrigation under ﬁve different irrigation durations and three water depths (2 mm, 20 mm, and 35 mm). Sphagnum peatmoss, coconut coir, and aged pine bark were tested at 67%, 50%, and 33% initial moisture (by weight). The objectives were to determine the impact of varying irrigation event durations (5, 10, 20, 30, 60 min) over a 60-min period, and the further inﬂuence of water depth and initial moisture, on the water capture abilities of peat, coir, and pine bark. The number of irrigation events depended on the irrigation event time of that experimental unit divided by the total time of 60 min, varying from 12, 6, 3, 2, and 1 event. Hydration efﬁciency was inﬂuenced by initial moisture content (IMC), water depth, pulsing duration, and inherent substrate characteristics (hydrophobicity/hydrophilicity). Initial MC had the largest impact on peat, regardless of water level or irrigation duration. Lower IMCs increased the hydrophobic response of peat, further reducing the amount of water the substrate was able to absorb. Pine bark had a 5–10% decrease in initial hydration between 67%, 50%, and 33% IMC, while coir’s hydrophilic nature reduced any IMC affects. At 50% IMC or less, coir had the highest volumetric water content (VWC) across all substrates, pulsing durations, and water depths. Water depth was found to increase initial hydration and ﬁnal hydration 6–8% across all substrates. These three materials had altered and varied water capture responses depending on the combination of treatments employed. This work demonstrated the effects of intensity and exposure on substrates and the need for more integrated research for improving water use efﬁciency on container crops. ﬂood


Introduction
As the global population continues to increase, demand on the limited water supply is expected to follow suit. With the future of water restrictions still unknown, the move towards more efficient containerized plant production systems is essential. To create an efficient system in container production, the substrate, irrigation system, and the container must be considered together as a whole. Soilless substrates were first introduced for container production to increase drainage by maintaining a high air-filled porosity as compared to mineral soils [1]. The higher air space in container substrates compared to mineral soil was used to decrease the hypoxic potential of the container, regardless of over-irrigating or increased precipitation. The side-effect of increased airspace is the practice of excess water application to limit the risk of under watering [2]. z Particle size distribution calculated on a dry weight scale using means of three oven-dried samples; y Coarse= particles that are greater than 2.0 mm in diameter; x Medium = particles that are less than 2.0 mm but greater than 0.5 mm in diameter; w Fines = particles that measure less than 0.5 mm in diameter; v Values are means of percentages of the total sample; u Statistics are determined down columns (denoted by an uppercase letter) and across rows (denoted by a lowercase letter) using Tukey's honestly significant difference to determine similarities and differences across all components.  Each of the three substrate components were tested at three IMCs. The most common and recommended MC for potting soils has a mass wetness of 1.0 g/g (50% MC). To test effects of MC, each component was also brought to half (0.5 g/g) and double (2.0 g/g) this normal level, which resulted in percent MCs of 33%, 50%, and 67% by weight. All substrates were then air-dried down to IMCs of 67%, 50%, and 33%. Initial MC and total weight of each sample were used to calculate how much water needed to be lost to reach IMCs of 67%, 50% and 33% IMC. Using a 160 cm × 49 cm × 69 cm four-tier PVC-enclosed dehumidifying chamber, substrates air dried until weight (g) loss equals number of mL's calculated to be removed from the substrate. Once the target IMC was reached, samples were transferred to a plastic bag and sealed to prevent further water loss, while allowing the substrate to reach moisture equilibrium after 24-48 h.

Particle Size
Particle size distribution ( Table 1) was performed on three, 50-g oven dried samples of each substrate using a total of 11 sieves. These sieves ranged in size from greater than 6.3 mm to less than 0.063 mm plus a bottom pan to collect fine particulates. The sieves were placed into the RX-29 Ro-Tap sieve shaker (278 oscillations/min, 150 taps/min; W.S, Tyler, Mentor, OH, USA). The sieves and pan were shaken for five minutes, and the particle fractions retained on each sieve and the amount collected in the bottom pan (representing the smallest particle fractions) were weighed.

Subirrigation System
This experiment was conducted using materials and modified procedures described by [9]. The required equipment consisted of a transparent cylinder, 5 cm i.d. × 15 cm·h −1 , with a mesh screen (mesh size 18 × 16; New York Wire, York, PA, USA), attached to one end, using rubber pressure plate rings (Soil moisture Equipment Corp., Santa Barbara, CA, USA) ( Figure 1A). The subirrigation method used to simulate capillary rise uses an Ebb and Flood irrigation unit (Hawthorn Hydroponics, Vancouver, WA, USA) 60.96 cm wide by 121.92 cm in length [26] (Figure 1). Water was introduced into this system via a faucet and controlled through a series of gate valves connected to the system ( Figure 1C). Water level was maintained at a continuous height with a flow rate of~21 L/min. To be able to control the height of the water while also having a steady flow into the unit, a standing copper pipe was cut to allow water to be held at heights of 2 mm, 20 mm and 35 mm above the surface of the unit at a steady state. Water levels were chosen to simulate three subirrigation conditions: 2 mm for trough methods, 20 and 35 mm for ebb and flood systems, where water levels are usually between 20 and 35% of the container height. The water level of 2 mm is also analogous to the RHP WOK method [27] where water level is held at a few millimeters in height.
The transparent cylinders were packed with each substrate component to have a Db within 5% of other samples of the same component. To achieve this, cylinders were filled with substrate (by weight) and then raised 12 cm off a flat surface, then tapped four times to bring the top of all 4 replications to a pre-measured line 10 cm from the base of the cylinder and 5 cm from the brim, representing 200 mL of substrate. Cylinders were then placed on an elevated mesh screen to optimize surface area exposure to water at about 1.7 cm off of the base of the flood unit. The transparent cylinders were packed with each substrate component to have within 5% of other samples of the same component. To achieve this, cylinders were with substrate (by weight) and then raised 12 cm off a flat surface, then tapped four t to bring the top of all 4 replications to a pre-measured line 10 cm from the base o cylinder and 5 cm from the brim, representing 200 mL of substrate. Cylinders were placed on an elevated mesh screen to optimize surface area exposure to water at abou cm off of the base of the flood unit.

Hydration Events
Each water application is referred to as a hydration event. Pulsing in this context means time of exposure to water based on a total time of 60 min. The hydration events tested were 5 min exposure over 12 events, 10min exposure over 6 events, 20 min exposure over 3 events, 30 min exposure over 2 events, and 60 min exposure in 1 event. All hydration events were tested at water heights of a 2 mm, 20 mm, and 35 mm above the sample base. Once placed on the mesh screen, the unit was filled with water. Water was allowed to fill until water poured into the copper piping fitted to the desired water level. At that time, water flow input equaled output, allowing constant flow of water without a change in water level. The substrates were held at a constant water level for the allotted event time (between 5 min and 60 min) to absorb water through flooding and capillary action. Once the event was finished, water was drained from the unit for one timed minute before each cylinder was weighed. The difference between final and initial weights was the amount of water captured by the substrate during that hydration event. This procedure was repeated based on specified time-allotted events (12, 6, 3, 2, 1 irrigation event(s)).

Container Capacity
After the final hydration event was complete and final weights were taken, CC was determined as a measure of the substrate's maximum hydration. To determine CC, the cylinders were returned to the ebb and flood unit for measurements following a modified version of the method described in the North Carolina State University Porometer Manual [25]. After samples were placed in the subirrigation unit, a 2 kg aluminum weight was placed on the top of each cylinder to prevent tipping because of buoyancy ( Figure 1A). The samples were then saturated from below by allowing water to flow in until it reached 1/3 of the height of the sample (3 cm from the base of the sample, 4.7 cm from the base of the unit). After waiting two minutes, water was allowed to flow again until reaching 2/3 of the height of the cylinder, or 6 cm from the base of the cylinder. After an additional two minutes, the water was applied until reaching the top of the sample within the cylinder ( Figure 1A), 10 cm from the base of the cylinder [6]. After saturating in the system for an additional 30 min, the water was drained, and samples were reweighed to record changes in weight (water captured and retained). Samples were then placed into a forced-air drying oven at 105 • C for 48 h to dry. Then, each sample was weighed, and dry weight was used to determine total water retained and moisture content.

Capture Rate
Water capture rate (CR) was calculated for subirrigated substrates using a modified version of the flow rate formula to account for variables in this experiment, the equation was written as (1): where CR is the amount of water captured by the substrate after one irrigation per unit time (mL/min), C i (initial capture) is the weight (g) of the substrate after the present irrigation event (minus the weight of the cylinder), C p (previous capture) is the weight (g) of the substrate (minus the cylinder) taken after the previous irrigation (for the first irrigation event, C p is equal to the pack weight of the cylinder (minus the weight of the cylinder), t is the amount of time per irrigation (minutes).

Capture Values
In order to provide both statistical and numeric comparisons, hydration efficiency of the substrates were examined using irrigation simulations and categorized in four ways: (1) initial moisture content, (2) water depth, (3) hydration event time and (4) pulsing effects. This allowed for the direct comparison of initial uptake and maximum uptake across single, and multiple events as affected by depth and initial moisture content. Data is presented for coir, peat and pine bark in Tables 2-4, respectively. Initial hydration was the amount of water absorbed by the substrate after one irrigation event and compared across all pulsing technique durations. Final hydration was the amount of water absorbed by the substrate after the last or longest irrigation event.
Statistics were determined using SAS v. 9.4 (SAS Institute; Cary, NC, USA). A Tukey's HSD test with alpha = 0.05 was used to identify differences and similarities between substrates at individual MCs and pulsing regimens. This test also determined the similarities and differences of CC, initial hydration, and final hydration across substrates, MCs, and pulsing techniques.

Particle Size
Substrate particle size analysis was performed on all three substrates, with the results displayed in Table 1. Coir represented the substrate with the highest percentage of particles smaller than 2.0 mm, showing 69.2% of all particles tested with pine bark representing the highest percentage of coarse particles with 57.0% Peat occupied the middle of the three substrates with 8.8% more coarse particles than coir, but 41.4% less than that of pine bark. Bulk density values were also attained by taking the substrate initial dry weight and dividing by the total volume of the cylinder, with peat, coir, and pine bark recording values of 0.13 g/cm 3 , 0.10 g/cm 3 , and 0.16 g/cm 3 , respectively.

Hydration Curves
Previously described by [26], using VWC and time as our variables, these curves were designed to illustrate the cumulative water absorbed by the substrate. Peat, coir, and pine bark are all represented on different figures with each cell representing the three IMCs under that treatment condition. The IMCs for this experiment of 33%, 50%, and 67% were all determined by weight. Furthermore, the hydration efficiency curves were determined by VWC to describe the amount of water contained in the substrate. These curves show a VMC reading at event zero and represent the percent VWC at the IMC. Therefore, the IMC of 50% by weight is 12% to 15% by vol for peat. For coir, the IMC of 50% by weight ranges from 9% to 11% by vol, and for pine bark (at 50% initial moisture) they are 16% to 18% by vol. The differences represent the density differences between the three substrate materials.

Coir
The hydration curves related to the treatments of coir identify a pattern related to IMC, water level, and time ( Figure 2). Regardless of IMC, coir was able to capture water, but the intensity at which it imbibed water was dependent on water level and IMC. At a depth of 2 mm, the difference between a single 5 min pulse and a 60 min pulse was as <4% VWC (Table 2). In fact, as IMC increased, the difference between 5 and 60 min lessened while water capture was nearly the same across all IMCs. Timed pulses of water impacted the amount of water captured by coir, increasing its total water captured by as much as 12% VWC at 2 mm and 30 min when pulsed six times. Representing the most water captured by coir at a 2 mm water depth. Raising the water level to 20 mm increased water capture bỹ 10% VWC, with the gap in water captured between single and multiple pulses lessening to just 7% VWC. As IMC intensity increased, capture increased for the single 5 min pulse, but for single and multiple pulses between 10 and 60 min, there was very little significant change. At a water depth of 35 mm, pulsing regimens do not return any greater water capture than a longer single pulse. Imbibed water does increase compared to 20 mm, with 50% IMC returning the greatest water captured. Capture rate (CR) was calculated (1) for each pulsing time, water level, and IMC of coir. CR was directly affected by water level and pulsing duration. The highest CR for coir was under a single 5 min pulse, with the maximum rate recorded at 35 mm and 5 min representing 14.27 mL/min. x-no data recorded as these pulses are derived from divisors of the total minutes (ex. A single 20-min pulse, two-10 min pulses, and four-5 min pulses).

Peat
Intensity of moisture preconditioning impacted the hydration curves of peat under lower moisture conditions (Figure 3). Time, IMC, and water depth all impacted the hydration of peat, with IMC effectively reducing water captured by 4×. At a depth of 2 mm, it was not until 67% IMC that peat began to capture water (Table 3), effectively capturing only 42% of its container capacity at its greatest capture. Regardless of water level, 33% IMC proved to be too low of a moisture level for peat to capture any water, with the low moisture also impacting the CC, keeping it below 30% VWC (Figure 3). Water capture increased with time, however pulsing showed little impact, with the greatest capture recorded after a single 60 min pulse. Increasing the water level to 35 mm resulted in an increase in water captured by~5% VWC (Table 3), with the greatest capture at 35 mm and 67% IMC. The intensity of hydrophobicity was an important factor in the amount of water captured by peat, limiting the amount of water being imbibed by the substrate at both 33% and 50% IMC. As water depth, time, and IMC increased, capture also increased. However, the CC of peat was largely affected by IMC and water level, with a CC as low as 15.2% volumetrically at 5 min and 2 mm. Container capacity increases as IMC increases, rebounding to~68% at 50% IMC and further to~80% at 67% IMC. Pulsing time did not have an effect on peat CC. CR was also calculated using the same Formula (1). It is clear ( Table 3) that peat, mainly at 50% IMC and 67% IMC, captured the majority of water within the first 10 min. Beyond that threshold, even at 67% IMC, peat does not gain much water. Based on Table 4, the maximum CR for peat was at 35 mm and 5 min at 9.49 mL/min after the first irrigation.

Pine Bark
Larger particle size and pore structure equated to a linear increased in water capture in pine bark, displayed in the hydration curves ( Figure 4). With event time and pulsing showing no significant impact, the water level and IMC were the two factors that affected the water captured by pine bark. At 2 mm, bark displayed errant capture characteristics, with more water captured at both 33% and 50% IMC than 67%, and the highest capture recorded at 33% IMC and 6 pulses of 5 min each at 25% VWC (Table 4). With water capture reducing as moisture level increases up to 67% IMC. Raising the water level to 20 mm increased water capture by~5% VWC at 50% IMC, with very little statistical differences across all treatments. Lastly, raising to 35 mm represented the greatest water captured at 39% VWC, and the closest any substrate came to its treatment CC, capturing 55% of its known CC. The CC of pine bark is consistent, regardless of treatment with only~10% difference between the lowest and highest recorded CCs. What is important to note is that pine bark did not show any response to the time or pulsing treatment parameters, slowly increasing water captured as water level was increased from 2 mm to 35 mm. Capture rate for each treatment showed the consistency of pine bark's ability to imbibe water. Compared to both peat and coir, pine bark captured the majority of water within the first 5min. the CR for pine bark did not increase with IMC, in most cases it actually decreased. With 33% IMC representing the highest CR for most pine bark treatments. However, the maximum CR of pine bark was at 35mm and 10min representing 11.26 mL/min during the first irrigation event.

Pine Bark
Larger particle size and pore structure equated to a linear increased in water capture in pine bark, displayed in the hydration curves ( Figure 4). With event time and pulsing showing no significant impact, the water level and IMC were the two factors that affected the water captured by pine bark. At 2 mm, bark displayed errant capture characteristics, with more water captured at both 33% and 50% IMC than 67%, and the highest capture recorded at 33% IMC and 6 pulses of 5 min each at 25% VWC (Table 4). With water capture

Discussion
From the hydration curves in Figures 2-4, it appeared that the intensity of substrate moisture preconditioning prior to the first irrigation and water level had the overall greatest effect on water capture of peat, coir, and pine bark. Peat was the only substrate that demonstrated fluctuating CC values based on IMC from as low as 15.2% at 2 mm, 5 min, and 33% IMC to 84.1% at 20 mm, 20 min, and 50% IMC. While pine bark and coir were less affected by treatments, representing ~10% between the lowest and highest CC of both

Discussion
From the hydration curves in Figures 2-4, it appeared that the intensity of substrate moisture preconditioning prior to the first irrigation and water level had the overall greatest effect on water capture of peat, coir, and pine bark. Peat was the only substrate that demonstrated fluctuating CC values based on IMC from as low as 15.2% at 2 mm, 5 min, and 33% IMC to 84.1% at 20 mm, 20 min, and 50% IMC. While pine bark and coir were less affected by treatments, representing~10% between the lowest and highest CC of both substrates. While all substrates increased water capture as both IMC and water level increased, peat had the largest increase in capture. With the 2 mm water level representing the substrates capillary water uptake, coir demonstrated the highest capture (Table 2) at 33% IMC and 50% IMC, with peat narrowing the margin of difference at 67% IMC.
Across all IMCs, coir was able to capture and retain water. However, IMC did play a role in the rate at which it was able to do so. At 50% IMC, there was very little difference in the water capture between 5 min and 60min of irrigation exposure. Again, water capture increased~10% VWC as water level increased, and the greatest capture happened at 35 mm. Coir is known to be normally hydrophilic; Abad et al. [7] characterizes coir as having a sponge-like ability to soak up water and be able to retain it within the pores of the substrates. This is, in part, because of the increased level of fines in coir compared to both peat and pine bark, between 0.5 mm and 2.0 mm coir has~20% more particles in that size class than both peat and coir (Table 1). Subirrigation uses capillary rise (along with flooding level) to facilitate water uptake into the substrate. Couple this with the hydrophilic nature and retention of its sponge-like ability at lower moisture conditions, coir seemed generally unaffected by pulsing time differences [13]. That process was also evident in the hydration curves in Figure 2, regardless of pulsing time coir captured the most water within the first five minutes of irrigating.
From the data in Table 3, there is evidence that IMC had the greatest impact on peat's ability to capture water at all water levels and pulsing times. As IMCs increased in peat, the substrate's ability to capture water increased, in a nearly linear fashion. Peat is known to express hydrophobic intensity at lower moisture levels [5] or from the material drying processes in the production of these substrates [28]. However, these hydrophobic expressions can hinder the ability of peat to rewet, taking more water and effort for the substrate to hydrate [28,29]. At 33% IMC, capturing any water whether this was 5 min of exposure over 12 events or 60min and 1 event proved difficult under the drier conditions. For perspective, the initial hydration for peat at 35 mm and 5 min events was 9.9%VWC, whereas coir under the same treatment conditions was 40.6%VWC, with pine bark reaching 33.2%VWC (Tables 2-4). The hydration curves showed the same trends for peat; that increase in IMC increases the effectiveness of irrigation. The main idea is that the capture potential of peat is nearly 50% less under lower moisture conditions, while having the most variable CC across all substrates tested.
Compared to both peat and coir, pine bark has the highest percentage of coarse particles, with~30% more particles above 2.0 mm than peat (Table 1). While a higher percentage of particles >0.5 mm has been shown to greatly influence air space and CC [30], the larger pore sizes created tend to have difficulty holding water after saturation [31]. Pine bark was comparably unaffected by water exposure variables pulsing and time and observed an increase in water captured as depth increased. Similar to both peat and coir, the higher the IMC and water depth, the greater the capture. Most notably for pine bark, is the ability of the substrate to capture~81% of the water throughout all irrigation events in the initial hydration under 5 min pulses and 33% IMC. No other substrate tested observed that level of efficiency under low moisture conditions.

Conclusions
Using preconditioning, and exposure (pulsing, depth, and time) as the metrics to describe the response of these substrates, the amount of water capture varied with different combinations of intensity and exposure. Coir exhibited the least difficulty capturing and retaining water, with the greatest water capture occurring at 35 mm. Peat was affected by IMC, regardless of water level or pulse duration with 33% IMC capturing the least amount of water. Most notably, the difference between water capture at 5 min and 60 min was negligible, with all substrates demonstrating~70% of the water taken up using the 60 min pulse within the first 5 min. Overall, these three substrates exhibited fundamentally varied abilities of capturing water in response to initial moisture conditions, water level, and irrigation pulsing time.