Morphophysiological, Enzymatic, and Elemental Activity in Greenhouse Tomato Saladette Seedlings from the Effect of Plant Growth-Promoting Rhizobacteria

: The tomato is a widely cultivated and consumed vegetable globally. Comarca Lagunera is an important tomato-exporting region of Mexico. Salinity is an abiotic factor that reduces productivity and increases production costs. To advance growing period, there is high demand for the sustainable production of seedlings. Plant-growth-promoting rhizobacteria (PGPR) are characterized by improving plant growth through different mechanisms and can be an option for reducing the misuse of chemical fertilizers. The importance of the application of strains, evaluating various inoculation methods (in seed, soil, foliar spraying, and root immersion), should be evaluated to propose biofertilization packages in a speciﬁc crop. Thus, the study aimed to determine the effect of PGPR ( Bacillus paralicheniformis , Acinetobacter guillouiae , Aeromonas caviae , and Pseudomonas lini ) vs. nutrient solution and distilled water in the seedlings stage of saladette-type tomato on morphophysiological variables, nitrate reductase (NR) enzyme activity, and plant minerals via tissue analysis under greenhouse conditions. The four PGPR were inoculated by different methods (inoculation in seed, sprinkling, and both) in saladette-type tomato seedlings under greenhouse conditions and evaluated in vivo 40 days after sowing for morphophysiological variables, such as seedling height; stem diameter; root displaced volume; fresh and dry weight matter of the leaves, stems, and roots; leaf area; and nitrate reductase enzyme activity. The effect of the inoculation of PGPR showed signiﬁcant results for Pseudomonas lini vs. the control, with 40% higher values, on average, for plant height, stem diameter, displaced root volume, and fresh weight of root, leaf, and stem. The response of enzymatic and mineral content in seedlings was variable with nutrient solution and signiﬁcant with distilled water. Studies related to the promotion of plants in the subsequent phenological stages of a tomato, considering the selected PGPR, should be considered in future research.


Introduction
Tomato (Solanum lycopersicum) is a widely cultivated and consumed vegetable, with global production of 243,635,433 t. In 2019, the main producer was China, with 125,739,004 t produced, and Mexico ranked tenth, with a production of 3,441,639 t [1]. At the state level, in 2019, Coahuila, Mexico, produced 121,579 t, with a value of MXN 816 million. In the Comarca Lagunera region, there are 1090 ha of greenhouses and shade houses for tomato production, generating a harvest of 145,769 t, with a yield of 135 t ha −1 and a production value of MXN 911,369,000 [2]. Nurserymen, who produce seedlings under special conditions, are an important sector in the total tomato production system, advancing sowing dates, and reducing production costs and environmental impacts [3]. Thus, they make the marketing windows effective. However, these nurserymen need to produce the seedlings in sustainable conditions, since the farmers who will acquire these plants must adhere to a production system in the field or in a greenhouse that uses ecological alternatives. This need to demand seedlings developed with sustainable tools is not only to introduce them to an organic production system; the soils of the Comarca Lagunera region present high salinities that, with the application of chemical fertilizers, exacerbate the problem of salinity. In this sense, the provision of seedlings through organic alternatives allows the use of biofertilization as an ecological and sustainable tool [4]. One alternative for biofertilization is based on plant-growth-promoting rhizobacteria (PGPR). Some rhizobacteria, as part of their metabolism, increase fertility and benefit plants; crop growth can be affected by mechanisms such as phosphate solubilization, phytohormone production, antibiotics, and other vegetative biostimulant compounds [5]. There are already some commercial rhizobacteria that the tomato seedling producer has turned to. However, because these rhizobacteria are isolated from different environments (not saline and good temperature), these microorganisms have not been effective in extreme environments, such as the Comarca Lagunera region. Therefore, there is a need to enlarge the knowledge of other microorganisms that adapt to edaphoclimatic conditions and can be used as future biofertilizers in extreme environments [6].
Research carried out by Lara et al. [7] indicates the importance of the application of native strains, showing that plant material inoculated with a native strain presents higher average heights, lengths of leaves, and notable vegetative development in the angleton grass plant (Dichantium aristatum Benth). Similarly, in addition to expanding the range of PGPR that can be obtained from a particular area, several inoculation methods can be used, particularly inoculation of the seed, soaking of the soil, foliar spray, and immersion of the root. The inoculation method for these new PGPR should be evaluated, not only to propose indigenous PGPR, but also to promote them with their respective inoculation method in a particular crop [8].
Based on the above discussion, the objective of this study aimed to determine the effect of PGPR (Bacillus paralicheniformis, Acinetobacter guillouiae, Aeromonas caviae, and Pseudomonas lini) in the seedlings stage of saladette-type tomato on morphophysiological variables, nitrate reductase (NR) enzyme activity, and plant minerals via tissue analysis under greenhouse conditions.

Inoculation in Seed and Production of Seedlings
The inoculation study of tomato seedlings was carried out under greenhouse conditions at the Technological Institute of Torreon, located between coordinates 24 • 30 and 27 • N, and 102 • 00 and 104 • 40 W, at an altitude of 1120 masl (meters above sea level). The vegetative material of tomato corresponds to the hybrid saladette type cv. Sahel, which is an indeterminate hybrid with a robust plant that produces high yields. The hybrid produces uniform fruits of good quality, with soft shoulders, shine, and firmness throughout the season, even in adverse conditions. Extra-large to large fruit are of intermediate maturity, and are ideal for national and export markets [9].
The treatments under study were designed from the combination of inoculation with four bacterial strains (LB1 = Bacillus paralicheniformis; NFB2 = Acinetobacter guillouiae; KB3 = Aeromonas caviae; KB4 = Pseudomonas lini) considering three methods of application (seedling spray (AP), seed inoculation (IS), and both (AP + IS) and two controls (distilled water and a nutritive solution) applied under irrigation mode, resulting in a total of 14 treatments, as described in Table 1. The distribution design of the resulting treatments was still completely random, with eight replications obtaining 120 experimental units. The four bacterial strains were donated by the microbial ecology laboratory from Universidad Juarez del Estado de Durango (UJED). Table 1. Halo-PGPR, and mode of application used in the production of tomato seedlings. After the reactivation of each bacterial strain, it was adjusted to a concentration of 1 × 10 8 CFU in 0.5% PBS. For seed inoculation, 200 tomato seeds were added to each of the bacterial cultures in a 50 mL Kitazato flask, and they were vacuum subjected to 600 mm Hg for 5 m [10]. Then, the seeds inoculated by vacuum infiltration of the bacteria were deposited in germinating plates of 1 m 2 containing 7 cm of peat moss (brand PRO-MIX GTX; base of fine granulation sphagnum peat of Canadian origin) as the medium of germination, and covered with black plastic until germination. The germinating plates were placed in a greenhouse at an ambient temperature of 27 • C and a relative humidity of 35%. After emergence, the trays were conditioned at 12 h light/dark. Irrigation with distilled water was carried out daily until 40 days after sowing (DAS).

Treatments
Regarding the spray application [11], each bacterial strain was adjusted to a concentration of 1 × 10 8 CFU in 0.5% PBS. Every eight days, aspersions were carried out until 40 DAS, covering 300 mL per plant with the bacterial strain under study. Treatment of the union of both types of inoculation (AP + IS) was the same as that indicated previously.

Irrigation and Preparation of Nutrient Solution
Irrigation with distilled water was applied to each tray to attribute the results to the evaluated treatments; half of the volume was applied in the morning and the remainder in the afternoon to maintain the seedlings in optimal humidity conditions. The water from Lagunera Comarca is rich in minerals; according to the classification of water for agricultural irrigation in Manual 60 of the US Department of Agriculture [12], the water is classified C2S1 of medium salinity [13]. In this sense, farmers usually apply a nutrient solution to avoid stress in the seedling stage. So, the chemical fertilization in this study was considered as a control, using a Steiner nutrient solution ( Table 2) and applying in a volume of 1 L per day for all seedlings throughout the experiment. Distilled water was considered the other treatment control. Seedling height was measured at the conclusion of the experiment at 40 DAS, based on eight repetitions per treatment. A STANLEY (USA) 8 m/26 tape measure was used, and the data for this variable are expressed in centimeters (cm). The stem diameter was measured using a metric and imperial scale Starrett 1223 (USA) Special Master Dial Indicator analog vernier, and the data for this variable are expressed in millimeters (mm). For the root displaced volume variable, a graduated cylinder was used as a level, after which the root was submerged and the volume of the root inside the cylinder was quantified; the data for this variable are expressed in milliliters (mL). Seedling fresh and dry weight (dry matter) of leaves, stem, and root, and leaf area were quantified once at the end of the experimental work; a VELAB VE-500 (USA) digital analytical balance was used, and the results obtained are expressed in grams (g). Dry matter was quantified at the end of the experimental work. The vegetative material was previously dried for 72 h in a drying oven at a temperature of 80 • C, and then weighed on a VELAB VE-500 (USA) digital analytical balance; the results for this variable are expressed in grams (g). Leaf area was obtained once at the end of the experiment. A foliar area integrator of brand LI-COR and model LI-3100 (USA) was used, and the results reported for this variable are expressed in cm 2 .

Enzymatic Activity
Nitrate reductase enzyme activity in vivo was assessed at the end of 40 DAS. Tomato seedling samples were collected and taken in containers with ice to avoid early wilting to the Laboratory of Plant Physiology and Nutrition of the Center for Research in Food and Development (CIAD) Delicias Unit, Chihuahua, for determination. The procedure used was an adaptation of the methods proposed by Joworski [14] and Mauriño [15]. The test was carried out both on the leaf blade and the root of the plant. Three enzymatic activities of nitrate reductase were tested: (1) endogenous reaction; (2) induced reaction with NO 3 − substrate; and (3) reaction with Mo as a cofactor. For the quantification of NR activity in vivo, 0.15 g of leaves and stem were weighed. Leaf discs were generated with a punch and introduced into 10 mL of infiltration medium, which was different depending on the determined NR activity: (a) in the case of endogenous NR activity, 10 mL of 100 mM potassium phosphate buffer pH 7.5 was used plus 1% propanol; (b) for NR activity induced with NO 3 − , 10 mL of potassium phosphate buffer 100 mM pH 7.5, containing 50 mM potassium nitrate plus 1% propanol was considered; (c) for NR activity induced with molybdate, 10 mL of potassium phosphate buffer 100 mM pH 7.5 was applied, containing 50 mM sodium molybdate dihydrate plus 1% propanol. Then, for each of the analyses separately, the samples were subjected to a vacuum process (8 bar) for 10 min in the dark; the samples were subsequently incubated at 30 • C in the dark for 60 min. The tubes were placed in a hot water bath at 100 • C for 15 min. For the determination of NR activity in vivo, the following were used: 1 mL of the aliquot (Metra); 2 mL of 1% sulfanilamide dissolved in 1.5 normal HCl; 1 g of sulfanilamide plus 20 mL of 35% HCl, dissolved in 100 mL of water; 2 mL of N-1-naphthyl-ethylenediamide (NNEDA) at 0.02%, dissolved in 0.2 normal HCl; and 20 mg of NNEDA dissolved in 100 mL of HCl, in this case water. After 20 min, the absorbance was read using a Jenway EW-83058-18 (UK) UV/Vis spectrophotometer at a wavelength of 540 nm, against a standard curve of NO 2 − between 0.25 and 2 µg ml −1 , following the method proposed by Hageman and Hucklesby [16]. The nitrate reductase activity in vivo was expressed in µM of NO 2 − formed by g −1 p. F. h −1 (micromoles of nitrites formed by grams of fresh weight in one hour).

Plant Tissue Elemental Analysis
Samples of fresh tomato seedlings were collected and taken to the Laboratory of Plant Physiology and Nutrition of the Food and Development Research Center (CIAD) Delicias Unit, Chihuahua, where they were subsequently washed and dried to determine the following minerals: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sodium (Na), manganese (Mn), nickel (Ni), iron (Fe), zinc (Zn), and copper (Cu).
For determination of the concentration of total nitrogen (Nt), 3 mg of sample (dry and ground) of each treatment was weighed in triplicate; 11 mg of pentoxide was added in an aluminum container and it was analyzed in a CHNS/O elemental analysis, performed using Thermo Scientific FLASH 2000 (USA) Organic Elemental Analysis (OEA) [17]. The quantification of phosphorus (P) was undertaken using colorimetry with a mixture of triacid and metavanadate molybdate ammonium. Determination of the concentration of potassium (K), calcium (Ca), and magnesium (Mg) was undertaken using atomic absorption with a digester mixture [18]. Quantification of the concentration of sodium (Na), manganese (Mn), nickel (Ni), iron (Fe), zinc (Zn), and copper (Cu) was undertaken using atomic absorption spectrophotometry with a digester mixture [19].

Microbiological Analysis
At the end of the experiments (40 days after sowing (DAS)), a microbiological analysis was carried out at the root and leaf level; 1 g of root and leaf (separately) were washed with distilled water and dilutions were made with tri-distilled water, before sowing in a Petri dish with Luria-Bertani medium lacking a nitrogen source free of nitrogen (LB) [20]. Bacterial treatments were sowed with 10 −5 dilutions. After sowing, Petri dishes were incubated. Colony forming units (CFU) were monitored and quantified at 48 h. Once the bacterial colonies were isolated, they were registered based on morphology and color, and the Rennie test.

Experimental Design
The data on the response variables were analyzed in a random experimental design by means of a variance analysis using the Statistical Analysis System statistical software version 9.2 (SAS Institute, Cary, NC, USA) [21]. Tukey's test was used (p < 0.05) to compare means.

Morphophysiological Responses, Enzymatic Activity, and Mineral-Elemental Response in
Seedlings, Due to the Inoculation of PGPR Associated with Distichlis Spicata 3.1.1. Seedling Height (PTH) The statistical analysis of the variable height of tomato seedlings produced in the greenhouse at 40 days after sowing (DAS) showed a significant difference among treatments (p ≤ 0.05). It was observed that the best treatment was the nutrient solution (14), and second was inoculation by P. lini in a combined way (seed + foliar spray) (12). The treatments with the lowest values turned out to be distilled water (13). Likewise, between the treatments (14 vs. 12), there was a difference of 12.9%, the latter exceeding treatment 13 by 67.2% (Table 3).

Stem Diameter (SDM)
A similar behavior to that observed in seedling height was observed in the stem diameter variable. It was observed that the best treatments were the nutrient solution (14) and the inoculation by P. lini in a combined way (seed + foliar spray) (12), without significant differences; between both of these treatments, there is a difference of 5.64%. However, a significant difference (p ≤ 0.05) was shown with the remaining treatments. The treatments with the lowest values turned out to be distilled water (13) and treatment number 1 (B. paralicheniformis in seed inoculation). Likewise, between the treatments (12 vs. 1), there was a difference of 79.91% (Table 3).

Root Displaced Volume (RDV)
In this variable, the inoculation was particularly evident when applied in combination with Pseudomonas lini (seed + foliar spray); compared with treatments 1 to 11, there existed differences with p ≤ 0.05. We found that treatment number 12 exceeded treatment 1 by 61.22%, and, although the Pseudomonas lini treatment (seed + foliar spray) performed better than treatment 14, there were no significant differences (Table 3).

Leaf Area (LA)
The statistical analysis of the leaf area at 40 DAS showed a highly significant difference between treatments. Nutritive solution (NS) showed better development, with leaf area 31.8% higher than seedlings treated with B. paralicheniformis in seed inoculation; however, among treatments inoculated, there were no significant differences, except with A. caviae and P. lini in inoculated seed. Moreover, it was observed that, among treatments, the best inoculation was using both forms (in seed and spraying seedling) ( Table 3).

Plant Fresh Weight and Plant Dry Matter
Analysis results of the root, leaf, and stem fresh weight at 40 DAS in tomato seedlings inoculated with PGPR showed significant differences among treatments. It could be seen that, in each fresh weight organ evaluated (root = R; leaf = L; and stem = S), treatment 11 (P. lini inoculated as a spray form) stood out, while the most affected treatments were 7 and 13 in R, L, and S (Table 3). On the other hand, in dry matter, the best treatment was inoculation with Bacillus paralicheniformis applied in the combination way (seed + foliar spray). Regarding nitrate reductase, the endogenous reaction quantifies the contribution of nitrate naturally assimilated by the plant to later reduce it to nitrite. A highly significant difference was obtained between treatments; the greatest effects were in 1 to 5, and 7 and 8 treatments, while, numerically, treatment 14 was the lowest. Bacillus paralicheniformis in seed inoculation showed 15.59% greater nitrate reduction than that of the treatment based on nutritive solution (Table 4). − + Mo). 1 = LB1 * seed inoculation; 2 = LB1 * seedling spray; 3 = LB1 * both; 4 = NFB2 * seed inoculation; 5 = NFB2 * seedling spray; 6 = NFB2 * both; 7 = KB3 * seed inoculation; 8 = KB3 * seedling spray; 9 = KB3 * both; 10 = KB4 * seed inoculation; 11 = KB4 * seedling spray; 12 = KB4 * both; 13 = DW * irrigation; 14 = NS * irrigation. Different letters within indicate significant statistical differences (Tukey; p ≤ 0.05).
According to the nitrate reductase enzyme, the reaction induced with NO 3 − as a substrate (NSB) quantifies the contribution of nitrate assimilated by the plant and subsequently reduced to nitrite. No significant differences were obtained in most of the treatments, except with Acinetobacter guillouiae and B. paralicheniformis using the two forms of inoculation (seed and spray form).
In the determination of the enzyme nitrate reductase in reaction with molybdenum as a cofactor (CMO), Mo serves as a bridge to bring the substrate and the enzyme together. In this case, it helps to make the reaction more stable for the NR enzyme. This quantifies the contribution of nitrate that the plant assimilates to later reduce it to nitrite. Analysis results showed variation and significant differences in treatments. Aeromonas caviae (9) was superior to other treatments, indicating that this strain in inoculation in seed and spray form works better with a cofactor that allows it to stabilize the reaction because it showed poorer endogenous results, or with the use of a substrate, in contrast to the other strains. The induced reaction of nitrate reductase with Mo as a cofactor and NO 3 − as a substrate for the NR enzyme quantifies the contribution of nitrate that the plant assimilates to later reduce it to nitrite. Notably, however, this reaction shows that, for P. lini inoculated in seed (treatment 10) to perform better, it requires more available nitrate and Mo as a reaction stabilizer.
P is an essential macro-element for plant growth. Phosphorus participates in metabolic processes, such as photosynthesis, energy and gene transfer, and the synthesis and degradation of carbohydrates [22]. The treatments with A. caviae (9), Bacillus paralicheniformis (3), and P. lini (12) were significantly superior to the nutritive solution and distilled water.
Potassium content showed a highly significant difference between the strains. Although the best treatment was with NS, it could be seen that the second-best treatment (8) was lower by 50.71%, while, with DW (13), it was 84.21% (Table 5).
Ca is a vital constituent of the cell wall and, consequently, is essential for meristematic activity. A highly significant difference in calcium content was found between treatments. The NS treatment also behaved as the best for the Ca element with p ≤ 0.05. Likewise, a similar behavior in the values was observed for treatments 1, 2, 4, 5, and 12 when the different strains were inoculated in seed and spray form ( Table 5).
The nutritional analysis of magnesium in the present study indicates a significant difference in both factors and their interaction. The strain KB4 (Pseudomonas lini) was superior in a combined way (seed + foliar spray) (12) by 73.52% compared with DW ( Table 5).
The results for sodium concentration showed that there was a highly significant difference between strains, and the bacterial application mode results were statistically equal. Again, the strain KB4 (Pseudomonas lini) was superior in a combined way (seed + foliar spray) (12) ( Table 5).
Manganese content showed a highly significant difference between treatments. The treatments with the highest values were the treatments NS, 7, 5, 6, 12, and 2 ( Table 5). Among the treated strains, the best was Aeromonas caviae with seed inoculation, surpassing DW by 55.81% (Table 5).
Nickel content did not show a significant difference between treatments; however, numerically, the best was NS. Strains KB3 (Aeromonas caviae) and KB4 (Pseudomonas lini) showed the highest nickel content among bacteria. The nutrient solution showed 4.92% higher nickel than the strain KB4 (Pseudomonas lini), which was the strain that showed the highest value among bacteria (Table 5). In the form of application, the combined treatment in P. lini showed the best values.
A highly significant difference in Fe content was obtained among treatments. The highest amount of iron was found in the NFB2 strain in the combined application form, with 456% greater iron than DW, and 219.48% greater iron than that of the nutrient solution (Table 5).
In terms of zinc mineral content, a significant difference was obtained between bacteria, and in the interaction of both factors. Higher values were found for the LB1 strain (Bacillus paralicheniformis) inoculated using the combined treatment. Zinc content was found to be 62.19% higher compared to the treatment of distilled water, and 25.56% higher compared to the nutrient solution (Table 5). Values for the form of application indicated that the mineral content of zinc was not affected by the form of application of the PGPR. Zinc is an important activating component of several enzymes involved in metabolic and biochemical activities.
A highly significant difference in copper content was obtained in the interaction of both factors. The strain KB4 (Pseudomonas lini) (11) in spray form showed better results when inoculated, with 82.86% more Cu content than that of the distilled water treatment, and 82.16% more than that of the nutritive solution (Table 5).

Microbiological Analysis (CFU)
In the microbiological count, a significant difference was found between the strains, the best treatments being the ones in which the strains NFB2 (Acinetobacter guillouiae), KB4 (Pseudomonas lini), and LB1 (Bacillus paralicheniformis) were applied. The opposite occurred for strain KB3 (Aeromonas caviae). Likewise, differences were also observed in comparison with the individual treatments (form of inoculation), which suggests that the combination of inoculations influences a greater number of growth-promoting microorganisms that favor biochemical and physiological variables, such as those evaluated in the present study (Figure 1).

Discussion
According to the inoculation of the four bacteria, considering the types of inoculation in the seedling stage, the results obtained agree with [23][24][25] and, although the trials have been carried out with other plants and other beneficial microorganisms, some inhibit the effects on germination [26]. However, other studies show positive effects with this type of microorganism [27,28] that also coincide with the results of the present study. The positive effects of these bacteria observed in the present study are apparently due to the production of substances like growth promoters, as reported in other studies [27][28][29][30][31][32]. Similar results were obtained by [33], in which significant differences, of up to 36%, were obtained in the height of tomato seedlings inoculated with PGPR compared to the non-inoculated control. Differences were previously found in the diameter of the watermelon and melon stem with the inoculation of Pseudomonas fluorenses, obtaining values 20.6% higher than the diameter of the control without inoculation [34]. This result is consistent with the present investigation, in which the diameter of the KB4 strain was greater than that of distilled water. The results obtained in our study exceed those obtained by Zulueta-Rodriguez et al. [32]. Noh et al. [33] found a significant difference with the inoculation of rhizobacteria in tomato seedlings, which had a 42.2% greater root volume than that of the control without inoculation, which indicates that inoculation with KB4 exceeds the results obtained by the aforementioned author. Aguado-Santacruz et al. [34] note that some species of the genus Pseudomonas produce an enzyme called 1-aminocyclopropane-1-carboxylate deaminase; increasing the activity of this enzyme immediately decreases the plant's production of ethylene and, therefore, significantly increases the growth of the root. Zulueta-Rodríguez et al. [32] obtained fresh weight values that were 49.56% lower for tomato seedlings inoculated with Pseudomonas putida. In combination with the values obtained in the present study, these results are not enough to indicate that Pseudomona strains increase the fresh weight of the seedling.
In addition, considering types of inoculation, Cisneros-Rojas et al. [35] identified similar values when inoculating coffee seedlings with a Bacillus strain, i.e., 59.70% higher dry weight than that of the control without inoculating. When analyzing bacterial root exudate and zoglea, high levels of phosphorus were detected. These were attributed to the ability of the Bacillus bacteria to solubilize phosphates and create synergies with other bacteria, like availability of nutrients, reduction of toxicity due to toxic metals, changes in pH, and production and / or stimulation of phytohormones, among others [35,36].
Espinosa et al. [37] mention that the application of Bacillus sp., combined with the use of a compost-based substrate, obtains large foliar areas. It is a good fertilization alternative for greenhouse tomato production, with an acceptable commercial quality and foliar areas. Rojas-Solís et al. [38] obtained similar and favorable results with Bacillus sp., compared with the strain Pseudomonas sp., in the growth promotion of tomato plants in a greenhouse. Although it is true that the present study was not conducted under salinity conditions, there are studies that show that plant growth can be promoted under these conditions, due to abilities such as reducing atmospheric nitrogen to ammonia (NF) through the synthesis of an enzymatic complex called nitrogenase [39].
The enzymatic activity of the plant is responsible for the formation of stable organic molecules that contribute to the stability of the physiology and biochemistry of the plant, and intervene in various cycles within it. Méndez-Gómez et al. [40] indicated that the positive effect of Bacillus paralicheniformis under a sprayed treatment is due to the relationship between nitric oxide and auxins at the beginning of the root development of seedlings; in metabolic pathways, nitric oxide and auxins are important for seedlings' growth. In various growth-promoting bacteria, nitric oxide can be produced under aerobic conditions and catalyzed in two ways, namely, via the enzyme nitrate reductase and the enzyme nitric oxide synthetase. Similarly, this positive effect on the plant caused by the LB1 strain (Bacillus paralicheniformis) via spraying occurs because LB1 is an aerobic bacterium and naturally induces the growth of seedlings. Coopens et al. [41] obtained a value of 0.14 µM of NO 2 − g p f h −1 from foliar application of microalgae to tomato seedlings in a greenhouse. However, this result was surpassed by that from the application of a Bacillus paralicheniformis strain via spraying, for which a value of 6.86 µM of NO 2 − g p f h −1 was obtained. The phytochemical analysis, or analysis of plant tissue, usually refers to the chemical analysis of leaves (foliar), root, and stem, but it is an analysis applicable to any plant tissue (twigs, flowers, fruits, others); this diagnostic technique is widely recognized to determine nutrient concentrations and thereby assess the nutritional status of the plant species. According to our results in N content, Sánchez-López et al. [42] showed in their work that, using strains of the genus Pseudomonas, the plant growth of tomato plants improved, registering greater absorption of nutrients. These results agree with those of the present study, because the strain KB4 belongs to the genus Pseudomonas. Studies related to other biological sources, such as that of Coppens et al. [41], used microalgae via a sprinkled application to tomato seedlings in a greenhouse, obtaining highest values (4.7% higher than the control) of nitrogen at 90 DAS. We consider that this difference in days could be overcome by Pseudomonas lini if the experiment had been extended for another 54 days. In addition to the results obtained with this nutrient in the present study, a significant difference was obtained in the application treatments. On the other hand, one of the most obvious effects of rhizobacteria in our study was observed in the increase in root mass, mainly in the number of secondary roots, which provides plants with a larger area for the absorption of water and nutrients. This is because PGPR can enhance plant growth by a wide variety of mechanisms, like phosphate solubilization, siderophore production, biological nitrogen fixation, rhizosphere engineering, production of 1-aminocyclopropane-1-carboxylate deaminase (ACC), phytohormone production, exhibiting antifungal activity, production of volatile organic compounds (VOCs), induction of systemic resistance, etc. [43]. Although this test was not carried out in this study, recent studies have shown that PGPRs containing ACC deaminase induce the production of longer roots, increasing the absorption of water [44,45]. Ribaudo et al. [46] mentions that, from the study of the relationship between the presence of ACC deaminase activity in PGPR and the inhibitory effect of ethylene on it, a possible role of the enzyme in the PGPR/PGPR interaction mechanism has been proposed in plants. At the same time, [11] report an activity of the ACC deaminase enzyme of 91 µmol α-KB / h / mg Pr of a PGPR isolated from the rhizosphere of D. spicata.
In P, K, and Ca contents, our findings agree with [22,47]; in addition, same authors cited that potassium is an essential nutrient that is absorbed by plants in greater amounts than any other nutrient, except nitrogen. Unlike most other nutrients, potassium is not incorporated into structures of organic compounds. Instead, potassium remains in an ionic form (K + ) in solution and acts as an activator of many enzymatic reactions in the cell [47]. K is involved in functions in nutrition and plant growth that influence quality. These include the regulation of metabolic processes, such as photosynthesis, and activation of enzymes that metabolize carbohydrates for the synthesis of amino acids and proteins, and facilitate cell division and growth by helping to move starches and sugars between parts of the plant [22]. With this in mind, our results agree with those of Azfal et al. [8] and Fukami et al. [48], when evaluating inoculation forms in nightshade plants.
In Mg, Na, and Mn content, our findings are similar to Azfal et al. [8], which indicates that a combination of inoculation, either to seed, substrate, or foliar spraying, was found to be favorable because the magnesium content in plants is favored by PGPR. The bestknown role of magnesium (Mg) in plants is its presence in the center of the chlorophyll molecule as a structural component of ribosomes and, for this reason, it is essential for photosynthesis. It is also involved in protein metabolism and is required for the maximum activity of almost all phosphorylating enzymes in carbohydrate metabolism [22], while in Na, the same authors [22] indicate that, when solanaceous glycophytes are influenced by PGPR, they show a certain tolerance to salinity that may exist in the soil solution or substrate, minimizing the osmotic or ionic effect on phenological variables, such as height, displaced root volume, leaf area, biomass, and dry matter, among others. Furthermore, sodium stimulates cell elongation growth and can replace potassium as an osmotically active solute, although, in excess, it seriously affects plant development [49,50]. According to Mn content, our findings agree with Shao et al. [50]. Moreover, they indicated that manganese acts as an activator of essential enzymes in growth processes, supports iron in the formation of chlorophyll, accelerates germination and maturation, and increases the use of calcium, magnesium, and phosphorus. The participation of Mn is mainly in the young parts of the plant, which are often the richest in Mn [50].
In Ni, Fe, Zn, and Cu analysis, our results showed that nickel (Ni) is actively absorbed through the plasmalemma in ionic form as Ni ++ . This element is essential for the function of the enzyme urease, which is important in the transformation of N [51,52]; Fe is a component of the enzymatic system that is present in oxidation-reduction reactions in the plant, and regulates respiration, photosynthesis, and reduction of nitrates and sulfates [22]. These activities are favored when the participation of PGPR occurs in plants, benefiting Zn as a functional, structural, or regulatory cofactor of a large number of enzymes, and playing an essential role in DNA transcription [53]. In participation with beneficial bacteria, zinc also performs the function of catalyzing the oxidation process in plant cells. In addition, it is vital for the transformation of carbohydrates and influences the formation of chlorophyll and auxins as growth-promoting compounds [54].
At the end of the experiments (40 days after sowing (DAS)) and a microbiological analysis considering the results, Peña et al. [55] indicates that this reduced CFU content may not be due to the incompatibility of the microorganism with the plant, but may be because of its property of slower growth. Currently, some methods are used to inoculate bacteria; the simplest is applying the bacteria in liquid suspension, either directly to the soil or to the seeds, or in a sprinkled way. However, there are others that should be evaluated and in different conditions (greenhouse and field) [56].
The results related to increases in the variables evaluated, under the given conditions and due to the beneficial effect of the bacteria studied in the tomato seedlings, suggest the possibility of using them at this stage. However, further research is needed on the fate of the inoculations in tomato so, in this sense, it is desirable to conduct complementary studies on this plant resource and its interaction with beneficial rhizobacteria.

Conclusions
Currently, sustainable sources are being sought in agriculture to displace the use of chemicals and improve yields. In the present study, the inoculation of Bacillus paralicheniformis, Acinetobacter guillouiae, Aeromona caviae, and Pseudomonas lini, compared with distilled water and nutritive solution in tomato seed, combined with a sprinkling in the seedling stage, in comparison with the control treatments (distilled water and nutrient solution), showed superior results in the enzymatic, morphophysiological, and nitrate reductase variables. In the elemental analysis of plant tissue, the results were variable, highlighting the combined form of inoculation for the bacteria. In particular, the results of the study allowed the highlighting of Pseudomonas lini and Bacillus paralicheniformis, especially when they were inoculated in a combined way (inoculation in seed and sprayed forms). However, studies related to the promotion of plants of the subsequent phenological stages of tomato should be considered in further studies.