Nitrogen Gap Amelioration Is a Core for Sustainable Intensification of Agriculture—A Concept

The main reason for the development of the yield gap in crop production is the inefficient management of nitrogen (N). The nitrogen gap (NG) cannot be ameliorated without an indication and quantification of soil characteristics that limit N uptake by a crop plant. The insufficient supply of N to a plant during its cardinal stages of yield formation is a result of two major-variabilities. The first is spatial variability in the soil characteristics responsible for water supply to a plant, also serving as a nutrient carrier. The second is a vertical variability in soil factors, decisive for pools of available nutrients, and their in-season accessibility to the grown crop. The long-term strategy for NG cover should focus first on soil characteristics (humus stock, pH, nutrient content) responsible for water storage and its availability to the currently grown plant. Diagnostics of plant nutrient availability should deliver data on their contents both in the topsoil and subsoil. The combined use of both classical diagnostic tools and spectral imagery is a way to divide a single field into units, differing in productivity. Remote-sensing techniques offer a broad number of tools to define the in-season crop canopy requirement for fertilizer N in homogenous field units.


Food Gap and Sustainable Intensification of Agriculture
The global human population, depending on the scenario, will reach 9-10 billion in 2050. Some prognoses of food requirements in 2050, based on the level of food production in 2005, fluctuate from 50% to 110% [1,2]. The analysis made by Hunter et al. [3] showed a much lower level of food demand in 2050 compared to 2010, ranging from 25% to 70%. The effective management of global food demand in 2050 should be a result of the simultaneous implementation of three complementary strategies, termed "mega-wedges" [4]. They are as follows: (i) filling the food production gap, (ii) decreasing food losses in the entire food chain, (iii) reduction in the worldwide food demand. The relative contribution of each particular mega-wedge in food security control has been assessed as 46%, 33.6%, and 20.4%, respectively. The second strategy does not include only direct losses of energy and proteins in the food chain, but also reduced productivity of soil and water (10.4%). This means that above 55% of the future food demand directly depends on efforts oriented on covering the food production gap. With respect to food losses, it is necessary to stress that the net excess of the global protein supply is almost equal to its intake by humans (36% vs. 44%) [5]. It can be, therefore, concluded that the management of the food production chain requires significant changes in agriculture. The conceptual (diagnostic, management, techniques, technology) preparation of the agriculture sector for a considerable increase in food production in the coming 30 years is the key challenge for the present generation.
The current level of food production is a function of two main factors, i.e., (i) actual crop yield, (ii) crop yield improvement. The actual yield of a particular crop in a strictly defined locality (field) is a result of the efficiency of production inputs under the course of on the improvement in N management. The required rate of food production increase to cover the food gap by 2050 can be achieved by an adequate supply of fertilizers, both nitrogenous, and those balancing N, i.e., containing P, K, Mg, S, and micronutrients. Scenarios of nitrogen fertilizers (N f ) consumption in agriculture to cover the food production gap vary 3-fold, i.e., from 85 Mt N y −1 to 260 Mt N y −1 [22]. In the model of N flows by Conijn et al. [23], the total consumption of N f will increase to 181 Mt y −1 , i.e., by 76% in 2050 as compared to 2010 (103 Mt N y −1 ). The increased N f consumption will lead, however, to a simultaneous increase in losses of its active compounds into the environment. The N loss in 2050 is projected to lie in the range of 102-156% with respect to 2010 [24].
Any increase in the production efficiency of both key agronomic factors, i.e., water and nitrogen, depends on the soil status of all the other production factors decisive for their efficiency. The production of phosphorus fertilizers to cover crop plant requirements for this nutrient is set to increase in the period 2010-2050 by 32% (from 17.9 to 23.7 Mt P y −1 ) [23]. An analysis made by Pradhan et al. [25] showed that to fulfill food production goals in 2050 the consumption of N, P, and K fertilizers will increase in the range of 45-73% for N, 22-46% for P 2 O 5 , and 2-3-fold for K 2 O, respectively, compared to 2010. The sustainable intensification of agriculture cannot be realized without taking into account other nutritional factors that limit the productivity of water and nitrogen, such as magnesium, sulfur, and micronutrients [26,27].
The principal challenge to the concept of sustainable intensification in agriculture is to develop effective diagnostic and management tools oriented to the increased efficiency of the applied fertilizer nitrogen. Its realization is a prerequisite for decreasing the pressure of losses of its active forms to the local and, as a consequence to the global environment. These twin objectives can be successfully realized, provided there is a recognition and a simultaneous amelioration of factors constraining the productivity of nitrogen both in the critical stages of yield formation by the currently grown crop, and resulting from the spatial variability of its supply to plants.

Water Limited Yield-WLY
The yielding potential of a particular crop plant expresses its genetic potential for the exploitation of solar radiation and CO 2 fixation [28]. Yield potential (Y p ), as proposed by Evans and Fisher [29], defines the maximum attainable yield of a crop cultivar grown under conditions of the non-limiting supply of nutrients, and effective control of pests and diseases. These growth conditions can be achieved provided the implementation of irrigation to the currently grown crop [30]. Water and nitrogen are classified as factors limiting yield [28].
The importance of water supply to crop plants during the growing season is wellknown to farmers. A temporary shortage of water is a natural feature of natural, i.e., non-irrigated agriculture [31]. Therefore, in practice a much more adequate term is water limited-yield (WLY, Y w ), i.e., Y p defined under natural water supply to crop plants. The really attained yield, in fact, depends on the unit productivity of water (water-useefficiency-WUE). This index expresses the amount of yield per total volume of evaporated and transpired water during a life-cycle of a currently grown crop [32]: WUE = Y a /ET a (1) where: WUE-water-use-efficiency, kg yield mm −1 or m −3 of water; Y a -actual yield, kg, t ha −1 ; ET a -water use (transpired and evaporated water), mm, m 3 . Two methods can be used as simple and practical tools to calculate Y w . The first is the FAS procedure (French and Schulz approach-FAS). This method is based on the assumption that the yield increase under the same environmental conditions is directly related to the increase in WUE [33]. The FAS method of water productivity calculation is composed of three components: (i) the maximum water productivity (TE), (ii) the quantities of water from current precipitation, (iii) the size of water resources in the soil volume rooted by the currently grown crop. The water-limited yield, (WLY) is calculated based on the equation: WLY = TE (R-ΣE s ) + WR (2) where: TE refers to the transpiration efficiency TE (TE = k/VPD; k-biomass/transformation ratio; VPD-vapor pressure deficit; R-the sum of rainfall during the growth period; E s -the seasonal soil evaporation, equal to 110 mm, WR -water reserves in the rooted soil volume. The yield gap can be defined as the difference between the yield resulting from the effect of water available to plants during the growing season, i.e., the water-limited yield (WLY) and the actual yield (Y a ): The key component in Equation (2) is the TE index. Originally, it was set for wheat grown in Australia at the level of 20 kg grain mm −1 of available water. Under favorable growth conditions, as stated by Passioura [34], this index can reach up to 30 kg grain mm −1 . As reported by Grzebisz et al. [26], TE is sensitive to the amount of supplied water and nutrients, ranging during the critical stages of spring triticale growth, from 14 to 39 kg grain mm −1 . The practical advantage of the Y w calculation, based on the FAS approach, is to quantify the yield loss due to inefficient water management. The fraction of the yield gap (YG f ) is a relative measure (a value, extending from 0 to 1) of the yield gap (YG) due to unfavorable growth conditions with respect to those created by the potential water supply (WLY) to the currently grown plant. As shown in Figure 1, the highest WLY was recorded in the wet year (1997). Irrigation applied to winter wheat during the critical stages of yield component formation, i.e., stem elongation and heading did not result in a yield increase. The highest values of the YG f , measured on the irrigated plots, indicate that water was not exploited by wheat due to the presence of other growth constraints. In contrast, the artificial reduction in the water supply (the treatment with imposed drought-D) to wheat during the critical period of yield component formation, resulted in a slightly lower grain yield. However, a much higher level of water exploitation was recorded, as indicated by much lower YG f indices, especially on the K fertilized plot (0.11 vs. 0.29 on the irrigated plot, I). The other two years were characterized by a natural water shortage during the stem elongation period (1998), or during the grain filling period (1999). As a result, the YG f , in general, approached zero, indicating that actual yields were close to the potential productivity of water. The negative values of YG f stress the effect of other growth factors, which increased water productivity. This phenomenon was observed on treatments with the imposed drought, revealed most frequently on the K-treated plots. The presented data corroborates the main assumption of the FAS procedure of WLY calculation that WUE depends on the amount of water available to the currently grown crop. The YG f index clearly reflects both the status of water management and soil fertility status.

Climatic Potential Yield-CPY
The maximum attainable yield of a particular crop, typical for the climatic region, is determined by the prevailing meteorological conditions. Based on this assumption Licker et al. [35], proposed to use the term, the climatic potential yield (CPY), instead of the less precisely defined, Yield Potential. The CPY can be defined as the maximum yield of a crop plant cultivar grown under a natural water supply, provided the optimization of other growth conditions. The yield gap (YG) is calculated based on the algorithm:

Climatic Potential Yield-CPY
The maximum attainable yield of a particular crop, typical for the climatic region, is determined by the prevailing meteorological conditions. Based on this assumption Licker et al. [35], proposed to use the term, the climatic potential yield (CPY), instead of the less precisely defined, Yield Potential. The CPY can be defined as the maximum yield of a crop plant cultivar grown under a natural water supply, provided the optimization of other growth conditions. The yield gap (YG) is calculated based on the algorithm: The fraction of the yield gap (YGf) is a relative measure (a value, extending from 0 to 1) of the Ya distance to the yield defined by the dominant weather conditions within a given climatic region (see Equation (4)). YGf values approaching zero, indicate that meteorological conditions during the growing season were favorable, allowing the attainment of the climatic yielding potential by the grown crop. The best source of CPY data are experiments conducted by accredited Experimental Stations. An example of CPYs and their respective indices are shown in Table 1. The differences between CPY and Ya for winter wheat were extremely pronounced. The calculated YGf accounted on average for 58% for winter wheat as compared to 26% for sugar beets. The low year-to-year variability of this index for both crops indicates (i) stability of the CPY for these two crops, irrespective of weather variability during the growing season, (ii) the presence of other growth factors affecting the actual yield. The main reasons for the recorded difference between winter wheat and sugar beets are soil conditions. In Poland, winter wheat, despite high requirements for soil fertility, is cultivated on a broad range of soil agronomic classes [36]. In contrast, sugar beets are cultivated on very fertile soils [37]. The second growth factor, significantly impacting the CPY of wheat, is the level of crop protection and the level of applied N. The higher input of agronomic measures resulted in a CPY increase of 13%. The fraction of the yield gap (YG f ) is a relative measure (a value, extending from 0 to 1) of the Y a distance to the yield defined by the dominant weather conditions within a given climatic region (see Equation (4)). YG f values approaching zero, indicate that meteorological conditions during the growing season were favorable, allowing the attainment of the climatic yielding potential by the grown crop. The best source of CPY data are experiments conducted by accredited Experimental Stations. An example of CPYs and their respective indices are shown in Table 1. The differences between CPY and Y a for winter wheat were extremely pronounced. The calculated YG f accounted on average for 58% for winter wheat as compared to 26% for sugar beets. The low year-to-year variability of this index for both crops indicates (i) stability of the CPY for these two crops, irrespective of weather variability during the growing season, (ii) the presence of other growth factors affecting the actual yield. The main reasons for the recorded difference between winter wheat and sugar beets are soil conditions. In Poland, winter wheat, despite high requirements for soil fertility, is cultivated on a broad range of soil agronomic classes [36]. In contrast, sugar beets are cultivated on very fertile soils [37]. The second growth factor, significantly impacting the CPY of wheat, is the level of crop protection and the level of applied N. The higher input of agronomic measures resulted in a CPY increase of 13%. CPY-the climatic yield potential; Y a -actual yield; YG-yield gap; YG f -the yield gap fraction; a1, a2-medium and high input of production measures (crop protection + higher N rate); SD-standard deviation; CV-coefficient of variation.

Partial Factor of Productivity of Nitrogen-PFP N
Nitrogen is, assuming the same meteorological conditions (precipitation) for a given locality (region), the key growth factor limiting yield [28,38]. Hence, the amount of N f or the whole N input at the beginning of the growing season becomes the principal independent variable, affecting both the plant growth rate during the growing season and its yield [39,40]. The efficiency of N f can be determined, using the concept of the partial factor productivity of fertilizer nitrogen, (PFP N ) [41]. This approach is frequently applied for making a country-to-country comparison [42]. The procedure to calculate the maximum attainable yield (Y attmax ), and in consequence, the nitrogen gap (NG) based on PFP N , requires data on the unit productivity of the applied N f under optimum growth conditions, i.e., the ample availability of nitrogen. The following set of equations can be used to calculate both indices: Nitrogen Gap NG = YG/cPFP Nf (9) To delineate the role of PFP Nf on yield, the critical value of PFP Nf has to be defined in the set of data obtained. The critical PFP Nf (cPFP Nf ) is calculated as the average of the third quartile (Q3) of PFP Nf values measured for each crop in a particular growing season. To determine the cPFP Nf , the calculated PFP Nf values are ranked in ascending order. The third quartile comprises values above the 75 th percentile. The cPFP Nf is the average of the PFP Nf values of the Q3. In the last step of the procedure, the nitrogen gap (NG) is calculated by transforming YG into the amount of the available N, but not used by the crop during the growing season [39]. The NG data can be used to prepare a graphical model of the efficiency of available N, i.e., mineral nitrogen present in the soil/crop plant system during the growing season of an actually grown crop. As shown in Figure 2, Y attmax for 32 tested fields in 2020, amounted to 8.663 t ha −1 . Theoretically, at this yield level, the soil N min content at wheat harvest is "zero". The excess of N min , as indicated by negative values of NG, leads to a yield decline and vice versa. The key question is to indicate a reason for the appearance of the NG and its size. In most studied cases, it was the excess of applied N f , concomitant with the low fertility of a soil agronomic class.

Growth Factors Efficiency and Yield
A crop yield depends on its potential to take in water and nutrients in well-defined stages of its growth [43]. Three groups of factors are responsible for the exploitation of the yielding potential of a particular crop cultivar: (i) weather conditions during the growing

Growth Factors Efficiency and Yield
A crop yield depends on its potential to take in water and nutrients in well-defined stages of its growth [43]. Three groups of factors are responsible for the exploitation of the yielding potential of a particular crop cultivar: (i) weather conditions during the growing season, (ii) soil fertility level, (iii) soil and crop management systems [35,43]. Weather conditions during the growing season in non-irrigated agriculture are the strongest environmental factor, significantly affecting year-to-year variability in yields [44]. Soil productivity has been indicated as one of the most important objectives listed in the sustainable development goals (SDGs) by the United Nations in the 2030 Agenda for Sustainable Development [45]. Mueller et al. [46] classified natural factors constraining plant productivity into three main groups, comprising: (i) soil moisture and its thermal regime-directly related to weather variability during the growing season and to in-season changes in water availability in the soil profile, (ii) root growth pattern and nutrient uptake patterns, (iii) field topography. Soil fertility can be defined as the inherent soil potential for supply of air, water, and nutrients to the currently grown crop plant in the required amounts and chemical forms, necessary for exploiting its yielding potential [47]. The key question with respect to the optimum ranges of growth conditions for a currently cultivated plant on a given field cannot be simply answered. The main reason is the huge number of factors that affect plant growth during the growing season, and its subsequent yield. Wallace and Wallace [43] suggest the existence of more than 60 factors, grouped into seven categories, including both biophysical soil properties (five groups), weather and soil, and finally crop management methods. Based on these evaluations, the production outcome, i.e., actual yield (Y a ) can be considered as a function of the climatic potential yield (CPY) and the efficiency of factors (E), affecting yield: The strength of each factor's impact on yield declines in accordance with its partial fraction, approaching 1.0. The value of 1.0 for a given factor indicates its optimum status with respect to the rate of plant growth, the formation of yield components, and the eventual yield.

Soil Fertility Constraints-Humus Content and Water Resources
During the first step in nitrogen gap (NG) amelioration, it is necessary to develop a set of effective diagnostic tools for recognizing the main soil characteristics-constraints that negatively affect plant growth. The second step should be oriented to working out a set of agronomic methods, allowing for the NG cover. Four groups of growth factors require a farmer's serious attention when evaluating the strength of soil factors that limit plant growth during the growing season. They are (i) total soil moisture amount and its availability to plants, (ii) the size of the in-season mineral nitrogen pool, (iii) pool of available nutrients responsible for N efficiency to plants during the growing season, (iv) plant accessibility to all sets of nutrients within the growing season.
The most important of these factors is water management before and during the growing season. World crop production systems to 60-80% depending on the amounts of water accumulated in the soil profile, strictly in the layer occupied by roots of the currently grown plant [48]. The quantity and availability of water stored in the soil profile to plants depend both on soil texture and its structure [49]). The capacity of the root zone (RZC) for water (the volumetric water capacity, VWC, at field capacity within a range of 10 and 1500 kPa suction) is measured for crop plants down to 100 cm [50]. In Europe, the RZC 100 for sandy soils is in the range of 50 to 100 mm, and for loamy soils from 100 to 200 mm.
The amount of storage and available water depends to a great extent on the content of soil organic matter (SOM, humus). In regions, or even in fields with a high contribution of sandy soil, the best option to increase VWC is to raise the humus stock (HS) in the soil profile. This operation can be only successful if the depth of the organic layer is increased [51]. It has been well-documented that the amount and resistance of soil humus to degradation processes is a function of soil texture [52]. Loveland and Webb [53] in an extensive review showed that the minimum content of humus required to maintain soil productivity is 1.7% for sandy soil (4% of clay particles) and almost 6% for clay loam (38% clay particles). The humus content in a particular soil is, in fact, a constant value, defined by the content of total silt and total clay. Based on this assumption Piéri [54] developed a soil degradation index, termed as the humus stability index (S), whose formula is as follows: where: S-index of soil humus stability, H-humus content (%), Si and C denote silt and clay content (%), respectively. The S index is a simple index to determine the current status of soil degradation. Four classes of soil sensitivity to degradation, based on the S index can be applied: (1) S < 5-structurally degraded soil; (2) 5 < S < 7-a great risk of soil structure degradation; (3) 7 < S < 9-a small risk of soil structure degradation; (4) S > 9-no risk of soil structure degradation. The S value above nine indicates that the humus content is at an optimum to protect the stability of the soil structure. In the next step in the procedure, evaluating the status of soil humus, the humus stock gap (HSG) can be calculated [55]: where: SHS SD -Standardized Humus Stock, t ha −1 ; HS A -Actual Humus Stock, t ha −1 ; HSG-Humus Stock Gap, t ha −1 ; OC-mean value of organic carbon content, kg m −2 ; 1.7-constant used to recalculate the OC content into humus; 10-constant, recalculating kg m −2 into t ha −1 . The yield gap/gain (YG/G), i.e., yield loss or gain, based on the humus content, can be calculated based on the formula: YG/YG = HSG · 15.6 (14) where: 15.6-constant, recalculating HSG into grain yield [56]. The OC data for the SHS SD calculation with respect to European soils were based on data reported by Batjes [57]. The simple calculation of HSG shows that a net increase in the humus stock of 1 g m −2 , which is equal to 17 t ha −1 of humus, results in a yield increase of 265.2 kg ha −1 . The potential yield increase can to a great extent be explained by the higher water-holding capacity of humus. As reported by Libohova [58] 1.0 g of humus holds up to 1.5 of water.
The main reason for the degradation of humus stock (HS) in arable soils is the rapid mineralization rate of the labile organic carbon pool, irrespective of soil management [59]. This process is accelerated by intensive NPK fertilization and soil plowing [60]. The regeneration of the HS in arable soil is a long-term process. The most effective amelioration strategies oriented towards an HS increase in arable soils, tested in long-term trials, rely mostly on intensive manure application. In European soils, as reported by Powlson et al. [61], expectations regarding the effect of manure are rather low. A yearly application of 10 t manure over 90 years can raise the humus content in a soil layer of 0.3 m only by 4.8%. As reported, however, by Szajdak et al. [62], a yearly application of 30 t ha −1 of manure to light soil over 38 years doubled the humus content. Despite the HS increase, no differences in rye yields were recorded compared to the effect of NPK application alone. The main practical disadvantage of this approach to HS increase, even in mixed farming, is its theoretical quality. This solution is not realized in farming practice due to a lack of manure. The option, applied in intensive agriculture, oriented only towards crop plant production, is the management of straw directly in the field. As frequently reported, this method of straw management can both increase the HS and yields of succeeding crops [63].

Nutrient Availability and Crop Plant Accessibility to the Nutrient Pools
The agronomic term nutrient availability refers to the amount of a particular nutrient taken up by the currently grown crop within a single growing season. Chemical tests of extractable nutrients are only an approximation of the amount of a given nutrient which potentially can be taken up by the crop plant [64]. In addition, the content of available nutrients is, as a rule, determined in the upper soil, limited usually to a layer of 0.2 m [65]. The term accessibility refers to the crop plant's access to soil pools of attainable nutrients within the growing season [64]. Plant access to a respective nutrient pool depends on the rate of root system growth, which is driven by the hormonal status of a plant, which to a great extent depends on plant access to nitrate nitrogen. A decreased supply of N-NO 3 to the aboveground plant parts affects the pattern of dry matter partitioning, leading subsequently to an increase of its investment into roots [66,67]. This crop plant strategy is oriented towards the capture of water, nitrogen, and nutrients supporting their use efficiency. It prevails in regions and soils sensitive to temporary water shortages [68,69].
The observed trends of crop plant response to irrigation are fully corroborated by the fact that ample water supply is a decisive yield factor, providing an optimum supply of nutrients, mainly N [46]. As shown in Figure 3, the yield of spring triticale decreased in accordance with the decreased amount of available water. However, the absolute and relative yield decrease, i.e., YG and YG f were much lower under K fertilized treatment. The main reason for the observed trend variability was the impact of K application on WUE. As a rule, the WUE-Eta trend reflected the trend of Y a , very well, but its steepness was lower on the K fertilized plots. The WUE-WLY indices showed different trends of dependence on K treatment. The index value, under conditions of K fertilizer application, increased in the opposite direction to the quantity of supplied water, i.e., from 23 kg grain mm −1 on the irrigated plot to almost 39 kg grain mm −1 on the plot with the artificial drought imposed during the stem elongation stage of triticale. It can be concluded that under natural precipitation, the yield gap can be ameliorated, at least partly, through the supply of nutrients like K, which exert a significant impact on water and nitrogen management by crop plants [26,70].
Fertilizer recommendations, except for mineral N (N min ), do not consider the content of available nutrients in the sub-soil. The total content of the majority of nutrients, taking K as a classic example, depends on the content of clay minerals [71,72]. The content of available nutrients is also sensitive to other soil characteristics, for example, soil pH (phosphorus, micronutrients); manure application, and the content of organic matter (nitrogen, phosphorus, micronutrients); fertilization, and cropping sequence (nitrogen, phosphorus) [65,[71][72][73][74][75]. The sub-soil has numerous functions; as a source of water, nitrogen, available pools of other nutrients, and as a natural milieu for the plant root system [76,77]. As shown in Figure 4, crop plants can penetrate the soil for K down to a depth of 0.90 m of the soil profile. In the presented case, the strongest soil depletion with the CaCl 2 -extractable K was recorded for winter oilseed rape. This phenomenon is corroborated by the fact that this crop has an extremely high requirement for K during the spring vegetation [78]. The same phenomenon was observed for phosphorus. As reported by Łukowiak et al. [79], crop plants can exploit the CaCl 2 -extractable P down to 0.9 m of the soil profile. The authors of this study documented that the recovery of 60% of the available P pool was concomitant with the highest yield of winter oilseed rape (WOSR). This figure may seem shocking, but it refers to the P content in the soil solution. An open question remains as to the contribution of the sub-soil P pool in the total P taken up by the currently grown plant. In the case of seed crops, for example, WOSR, an important part of P is taken up by plants following the onset of flowering [80]. A permanent application of P fertilizers, as documented for long-term experiments, leads to the enrichment of the sub-soil with P. As a consequence of this operation, this pool becomes a considerable source of P for high-yielding crops [81]. It can be, therefore, concluded that chemical tests for the soil pools of nutrients in the subsoil cannot be limited only to the N min content [82,83]. It has been recently documented that the simultaneous determination of N min and some other key nutrients in the CaCl 2 -solution is a source of knowledge of both their pools and the occurring relationships between them [82,83]. drought imposed during the stem elongation stage of triticale. It can be concluded that under natural precipitation, the yield gap can be ameliorated, at least partly, through the supply of nutrients like K, which exert a significant impact on water and nitrogen management by crop plants [26,70]. Fertilizer recommendations, except for mineral N (Nmin), do not consider the content of available nutrients in the sub-soil. The total content of the majority of nutrients, taking K as a classic example, depends on the content of clay minerals [71,72]. The content of available nutrients is also sensitive to other soil characteristics, for example, soil pH (phosphorus, micronutrients); manure application, and the content of organic matter (nitrogen, phosphorus, micronutrients); fertilization, and cropping sequence (nitrogen, phosphorus) [65,[71][72][73][74][75]. The sub-soil has numerous functions; as a source of water, nitrogen, available pools of other nutrients, and as a natural milieu for the plant root system [76,77]. As shown in Figure 4, crop plants can penetrate the soil for K down to a depth of 0.90 m of the soil profile. In the presented case, the strongest soil depletion with the CaCl2-extractable K was recorded for winter oilseed rape. This phenomenon is corroborated by the fact that this crop has an extremely high requirement for K during the spring vegetation [78]. The same phenomenon was observed for phosphorus. As reported by Łukowiak et al. [79], crop plants can exploit the CaCl2-extractable P down to 0.9 m of the soil profile. The authors of this study documented that the recovery of 60% of the available P pool was concomitant with the highest yield of winter oilseed rape (WOSR). This figure may seem shocking, but it refers to the P content in the soil solution. An open question remains as to the contribution of the sub-soil P pool in the total P taken up by the currently grown plant. In the case of seed crops, for example, WOSR, an important part of P is taken up by plants following the onset of flowering [80]. A permanent application of P fertilizers, as documented for long-term experiments, leads to the enrichment of the sub-soil with P. As a consequence of this operation, this pool becomes a considerable source of P for high-yielding crops [81]. It can be, therefore, concluded that chemical tests for the soil pools of nutrients in the subsoil cannot be limited only to the Nmin content [82,83]. It has been recently documented that the simultaneous determination of Nmin and some other key nutrients in the CaCl2-  [26]. Legend: IR, RF-irrigated and rainfed water treatments; FK+M, ST+H-water shortage imposed at * stem elongation + heading; ** flowering + early milk. WUE-water Use Efficiency; Eta-calculated based on evapotranspiration; WLY-calculated based on FAS approach [33]. solution is a source of knowledge of both their pools and the occurring relationships between them [82,83]. The vertical trends in nutrient content variability need to be considered when working out fertilization recommendations. It has been well-documented in literature that the sub-soil significantly impacts both the profile of crop rooting and in consequence the uptake of water and nutrients [76]. Rooting depth is not a constant pattern, even for the same crop. It changes due to the impact of numerous biophysical, environmental, and also soil and crop management factors [84]. The rate and habit of the root system, similarly to the shoot, undergo temporal changes during plant development and in response to its nutritional status [85]. The depletion of N-NO3 at the onset of WOSR flowering in the soil layer of 0.9 m depth is a prerequisite of high yield [80]. The efficient uptake of NO3 − ions depends on the respective concentration of K + in the soil solution [86]. For the low-yielding WOSR plantation P resources in the vegetative organs at the onset of flowering are sufficiently high to cover the requirements of the growing seeds. A high-yield of seeds can be achieved, but only provided there is efficient remobilization of P from vegetative tissues, and its simultaneous uptake from the subsoil [77,79,87]. It is necessary to take into account two other important facts. The first refers to the growth status of the root system. The onset of flowering results in the progressive root system dying, i.e., the rate of root mortality is higher than the appearance of new roots [64,88]. It is necessary to stress that the uptake of both, K and P depends to a much greater degree on the root elongation rate than on the concentration of both ions in the soil solution [89]. So far, the routine fertilizer recommendations have neglected the vertical variability of factors responsive to nutrient uptake by crop plants. This knowledge gap is one of the key reasons for the differences in the prognosis of crop production intensification in a sustainable way.

The In-Season Management of Nitrogen-Cardinal Growth Stages
The first task in the reorientation of the crop production system is to calculate the total requirement of a currently grown plant for nitrogen. This can be calculated for an average yield harvested on a particular field or based on the potential yield of a given The vertical trends in nutrient content variability need to be considered when working out fertilization recommendations. It has been well-documented in literature that the subsoil significantly impacts both the profile of crop rooting and in consequence the uptake of water and nutrients [76]. Rooting depth is not a constant pattern, even for the same crop. It changes due to the impact of numerous biophysical, environmental, and also soil and crop management factors [84]. The rate and habit of the root system, similarly to the shoot, undergo temporal changes during plant development and in response to its nutritional status [85]. The depletion of N-NO 3 at the onset of WOSR flowering in the soil layer of 0.9 m depth is a prerequisite of high yield [80]. The efficient uptake of NO 3 − ions depends on the respective concentration of K + in the soil solution [86]. For the lowyielding WOSR plantation P resources in the vegetative organs at the onset of flowering are sufficiently high to cover the requirements of the growing seeds. A high-yield of seeds can be achieved, but only provided there is efficient remobilization of P from vegetative tissues, and its simultaneous uptake from the subsoil [77,79,87]. It is necessary to take into account two other important facts. The first refers to the growth status of the root system. The onset of flowering results in the progressive root system dying, i.e., the rate of root mortality is higher than the appearance of new roots [64,88]. It is necessary to stress that the uptake of both, K and P depends to a much greater degree on the root elongation rate than on the concentration of both ions in the soil solution [89]. So far, the routine fertilizer recommendations have neglected the vertical variability of factors responsive to nutrient uptake by crop plants. This knowledge gap is one of the key reasons for the differences in the prognosis of crop production intensification in a sustainable way.

The In-Season Management of Nitrogen-Cardinal Growth Stages
The first task in the reorientation of the crop production system is to calculate the total requirement of a currently grown plant for nitrogen. This can be calculated for an average yield harvested on a particular field or based on the potential yield of a given cultivar in the same climatic region. Two additional components have to be taken into account to make a reliable estimation of the total N requirement by the currently grown cultivar. The first is nitrogen concentration in the main product, for example, seeds or grain. There is still ongoing scientific discussion with respect to the extent to which N concentration in seeds or grain is a conservative, i.e., genetically, or environmentally governed trait [90][91][92]. The second component refers to the partitioning of N taken up by the crop between the main yield, and its by-product, for example, between grain and straw. At harvest, this process refers to the value of the nitrogen harvest index (NHI), which is a conservative trait of seed crops [11,93]. Total N input (N i ) in the soil/crop system for an assumed yield of grain/seeds is calculated based on the algorithm: (15) where: N i -nitrogen input, kg ha −1 ; Y CPY -climatic potential yield of the grown cultivar, kg ha −1 ; G Nc -grain/seed nitrogen concentration, kg t −1 ; NHI-nitrogen harvest index, a value in the range of 0.6-0.8.
The key objective of nitrogen fertilizer (N f ) application is to synchronize its application time with the crop plant requirement. The dominant factor is the stage of plant growth and the required content of N, which progressively increases with the crop growth. The right determination of the N f dose in the critical stage of yield formation is, therefore, the decisive factor in the exploitation of the yielding potential of the currently cultivated crop. The crop demand for N in a particular growth stage depends on the rate of plant biomass growth, primarily driven by temperature and water supply [94]. The sum of physiologically active temperatures (GDD), and water and nitrogen supply are major factors for the quantity of biomass produced by the crop during the respective phase [95]. The rate of seed crop growth throughout the vegetative season is not constant. Based on this criterion, three mega-phases can be distinguished, named as canopy foundation (CF), yield component construction (YCC), and yield realization (YR) [96]. The first two periods describe the vegetative part of the plant life cycle and the third one its reproductive phases ( Figure 5). The shape of the dry matter accumulation curve can be described mathematically using very sophisticated models [97]. In cereals, the CF period, extending from sowing up to the end of tillering, is responsible for the number of tillers per plant. The course of dry matter accumulation is best described by the exponential regression model. The YCC period, extending from the beginning of stem elongation up to flowering, is responsible for the set of flowers. The rate of dry matter accumulation during this period is best described by the linear regression model. The dry matter yield of wheat at the end of the CK2 can be used to make a prognosis of grain yield [98].  The yield realization (YR) period commences from the onset of flowering and persists up to the physiological maturity of the plant. It can, however, be divided into two parts. The first part extends from flowering up to the watery stage of the seed/grain plant growth, i.e., to BBCH 71 (15% of seed/grain DW). It has well been documented that nitro- The borderline stages of plant growth, which occur between the mega-phases of CF and CYC, and between the CYC and YR periods, can be named Cardinal Knots (CKs), i.e., CK1 and CK2, for the first and second borderline of consecutive mega-periods, respectively. These two CKs are decisive stages for yield component formation. For example, as shown in Figure 6, the content of soil nitrate-nitrogen (NN) during the YCC period of oilseed rape undergoes a strong depletion (=N uptake by WOSR during the YCC period). The recorded NN depletion significantly affected the seed density, which was the key yield component, determining seed yield (R 2 = 0.89, p ≤ 0.001). What is most important, however, is the fact, that the dependence obtained clearly defines the rosette stage (BBCH 30) as the decisive stage for the yield prognosis (NN op = 163.3 kg ha −1 for Y amax = 77.6 · 1000 seeds m −2 ). The result obtained indicates that in farming practice, the time of N f application has to precede the BBCH 30. In maize, the second rate of N f is applied, based on N min determination, at the 5th leaf stage [99,100].
The yield realization (YR) period commences from the onset of flowering and persists up to the physiological maturity of the plant. It can, however, be divided into two parts. The first part extends from flowering up to the watery stage of the seed/grain plant growth, i.e., to BBCH 71 (15% of seed/grain DW). It has well been documented that nitrogen supply to a seed crop, like cereals and oilseed rape, significantly affects the degree of yield component expression. The N pool accumulated by the seed plant during its vegetative growth, i.e., before flowering, considerably impacts the number of seed/grain per field unit area (physiological sink capacity) [101][102][103]. The second part of the YR period, which begins from the early milk-stage (BBCH 72) and finishes at physiological plant maturity (BBCH 89/90), is termed as the grain/seed filling period (GFP, SFP). The course of dry matter accumulation can be described by different mathematical models, but the linear or quadratic dominate. During this particular period, grain/seed reaches its final individual weight [104].  The yield realization (YR) period commences from the onset of flowering and persists up to the physiological maturity of the plant. It can, however, be divided into two parts. The first part extends from flowering up to the watery stage of the seed/grain plant growth, i.e., to BBCH 71 (15% of seed/grain DW). It has well been documented that nitrogen supply to a seed crop, like cereals and oilseed rape, significantly affects the degree of yield component expression. The N pool accumulated by the seed plant during its vegetative growth, i.e., before flowering, considerably impacts the number of seed/grain per field unit area (physiological sink capacity) [101][102][103]. The second part of the YR period, which begins from the early milk-stage (BBCH 72) and finishes at physiological plant maturity (BBCH 89/90), is termed as the grain/seed filling period (GFP, SFP). The course of Post-flowering N uptake: PNUP = N t − N fl , kg ha −1 (18) where: N hv -N amount in vegetative organs of crop canopy at harvest, kg ha −1 ; N fl -N amount in vegetative organs of crop canopy at the onset of flowering, kg ha −1 ; N t -total N amount in crop canopy at harvest, kg ha −1 .
The patterns of N accumulation by the plant between particular CKs during the growth of a crop are the basis for a build-up of an efficient strategy of the in-season N status correction. Any free choice of N f timing and its dose, as frequently observed in classic or even modern fertilization programs, does not fit the crop requirements for N, being the main reason for its inefficiency. A sound strategy of N management in seed crops, despite an almost similar accumulation pattern throughout the life cycle, is crop-specific. For example, in bread wheat, the key period of yield component formation extends from CK1 to CK2. During this period, the requirement for N results from both (i) the number of grain per unit area, and (ii) the required protein content in grain [106]. Consequently, a nitrogen fertilization strategy based on the correction of the plant N status in both CK1 to CK2 should also take into account protein concentration in grain. As a rule, any increase in the number of grains per unit field area results in a protein concentration decrease [107]. Therefore, any fertilization strategy, oriented to the increase in the crude protein concentration, requires an extra N f dose, which should be applied at the end of the CK2. In maize, its nutritional status at the 5th leaf stage, which slightly precedes CK1, is decisive for the degree of yield component expression [108,109]. Nitrogen fertilizer should be applied just at such a time preceding this cardinal stage of maize growth because it affects the potential number of leaves and cobs. Nitrogen status in maize at the CK2, before the beginning of flowering, is important for the yield development, but it has only a predictive value [110]. In farming practice, it makes no sense to apply N f at this particular stage because the yield was already fixed in much earlier stages of maize growth.
Nitrogen efficiency depends on the supply of other nutrients needed for its uptake and utilization [70,[111][112][113]. It is necessary to pay attention to the fact that the accumulation of K by major crop plants, like wheat, oilseed rape, sugar beets, or potato, is as a rule higher as compared to N [114,115]. The maximum K uptake by high-yielding WOSR reaches its maximum during the phase of the main stem elongation [77,78]. It can, therefore, be concluded that an efficient uptake of K from the subsoil by some crops, in comparison to N, is a necessary condition for the effective uptake of nitrogen. As recently reported by Grzebisz et al. [80], the efficient uptake of NO 3 ions by WOSR depends on the adequate concentration of K and other nutrients, such as Ca and Mg in the sub-soil. All these nutrients are responsible for the development of yield components by crop plants.
One of the most important priorities in the breeding of crop plants is to increase the uptake efficiency of nutrients, especially of N and P from soil. Efficient acquisition of water and nutrients is required for the realization of both production and environmental goals. The efforts of key breeders have recently focused on the improvement of root system traits through [116][117][118][119]: (1) Accelerating the early rate of root growth of short-season crops, for example, vegetables; (2) Increasing root density in the topsoil to increase uptake of freshly applied fertilizers; (3) Deeper rooting of crops grown in areas vulnerable to nitrate-nitrogen leaching. (4) Stronger root branching to increase exploration of soil zones rich in mineral nitrogen; (5) Increasing the density and length of root hairs for effective acquisition of phosphorus and potassium by a currently grown crop; (6) Improvement in mycorrhiza association for effective acquisition of phosphorus and micronutrients.

Yield Gap Recognition and Diagnosis of Limiting Factors
An efficient N management strategy should be based on three major variables that affect the plant growth of a particular crop. Yield variability within a field is a result of (i) the stage of a crop plant growth, (ii) spatial variability in N uptake by plants, (iii) vertical variability in soil N pools, and a plant's access both to these pools and other nutrients responsible for N use efficiency. It is necessary to assume that the expression of yield components is a result of the growth pattern of a crop plant encoded in its early stages [80,120]. The principal difficulty in determining the right N f dose is the number and strength of plant growth limiting factors with respect to their spatial variability on the field. Yield, in fact, its spatial variability within a field, is the ex-post result of (i) the degree of yield constraints recognized during the growing season, (ii) the efficiency of production measures applied to ameliorate constraints limiting plant growth and yielding. The spatial variability of yield is the main reason for the necessity for dividing the entire field area into zones that differ considerably in productivity [121]. The key challenge is to find a criterion for the particular field division into zones of the same level of productivity.
The target of modern agriculture is to work out and implement a set of highly reliable diagnostic tools which will be capable of defining the methods and approaches to the efficient use of N f , that are in accordance with the concept of sustainable agriculture [21,122]. This concept, taking the field as the key production unit, is based on three main objectives: (1) fulfilling the food production gap, in fact, ameliorating the nitrogen gap; (2) achievement of a satisfactory level of applied production measures; (3) a significant decrease in the negative impact of applied Nf on the environment.

Nitrogen Use Efficiency-Limiting Factors
The most important task for a farmer is to establish a hierarchy of factors affecting the yield gap in a particular field and recognizing their depth. Irrespective of the climatic zone, the plant growth rate and development of yield components depend on the supply of N and its use efficiency [38]. Nitrogen use efficiency (NUE) defines the amount of the main product, for example, seeds, grain, roots, tubers, per unit of supplied N [123]. This N index is composed of two sub-units, i.e., nitrogen uptake efficiency (NUtE) and nitrogen utilization efficiency (NUtE), presented as The simplified interpretation of the first part of this equation, frequently used by crop breeders, mainly focuses on the amount of soil available nitrogen, i.e., to the low-and high-N input growth environment [124]. In fact, the amount of available N is a strong factor discriminating plant crop genotypes [125]. Nitrogen uptake and its subsequent transformation into plant biomass, i.e., NUtE, depends both on the physiological potential of a plant to take up N from its soil resources, and on soil factors limiting the rate of its uptake and subsequent utilization by a plant [113]. The spatial variability of yield clearly indicates that the amount of N taken up by plants of the same cultivar, i.e., having an identical yielding potential, is also spatially variable. Plants suffering from an N shortage due to their lower supply during the cardinal stages of yield formation are not unable to capture the same amount of solar energy compared to those growing in conditions of ample water and N supply. As a result, these plants are not capable of achieving the rate of growth as determined by the supply of light energy [126]. The ex-post formulated questions are: (i) at what stage of crop plant growth does the yield formation become limited by a shortage of N? (ii) what is the reason for the insufficient N uptake? The first question has been thoroughly discussed in Part 4. of this review. The second question should focus the farmer's attention on factors pertaining to the plant potential at a particular stage of its growth to access water and nutrient resources.

Diagnostic Tools for the In-Season and Spatial Yield Gap Control
The recognition of the yield gap and its size requires an implementation of a set of analytical tools with a capacity to make a reliable diagnosis of factors that limit plant growth and yield. A significant improvement in NUE is possible provided there is a reliable quantification of soil characteristics limiting both N uptake and its utilization by the currently grown plant. Hence, a single field is considered a basic unit in any diagnostic procedure.
The key characteristic of the yield of a currently grown crop is its spatial variability (Figure 7). The hierarchy of factors decisive for NUE is well recognized, but not always taken into account by farmers and their advisors. The primary tasks, aimed at an improved strategy for spatial N management, are to polygonize the field based on: (1) the climatic yield potential (CYP), and the water-limited yield (WLY); (2) the total requirement of the crop for nitrogen; (3) primary soil characteristics, assuming a homogenous regime of water and nitrogen supply to the (4) currently grown crop during its critical stages of growth; (5) soil pH and the content of active aluminum, as the key factor limiting the root system growth of crop plants; (6) contents of available nutrients, assuming a homogenous regime of their supply to the currently grown crop.
It is necessary to create a broad set of zonation maps with respect to soil and crop characteristics to get an operational tool to establish homogenous field production units (HFPUs) [127,128]. Therefore, the soil water capacity and its availability to the currently grown crop determine the first criterion of the field zonation. The primary factor affecting the diffusion of ions toward the plant root in the soil is the content of available water [50,58]. This factor shows a strong spatial and vertical variability within the growing season, significantly affecting crop growth at cardinal stages of yield component formation [129]. As discussed in Part 3, the amount of available water is defined by the content of colloids and soil structure. Hence, the main reason for existing variabilities in the available water content is the content of mineral colloids. The primary data can be collected using the classic diagnostic procedures of soil analysis. The working out of zonation maps requires the implementation of geostatic methods [130,131]. The advantage of the classic methods of basic soil characteristic determination is the necessity to analyze water and nutrient resources present both in the topsoil and in the sub-soil which is rooted by a currently grown plant [80,120]. A sequential determination of the N content along the growing season of a plant is a primary tool for its N status assessment. As a rule, during the vegetative growth of the seed crop, the proportion of active metabolic tissues (leaves) decreases stage-by-stage with respect to structural ones (stem). Consequently, the total N concentration in plant tissues decreases during vegetative growth, but its content in the plant progressively increases, reaching the highest value just before the onset of flowering (CK2). The temporal variability in relationships between crop dry matter biomass and N concentration is described by the following set of equations [132]: (1) Nc-the critical N concentration, % or g N kg −1 DM (2) NNI-Nitrogen nutrition index, NNI = Na/Nc (21) where: W-crop biomass, t ha −1 ; a and b-estimated parameters. The Nc curve is expressed as a power function, but its parameters are both crop and growth condition specific. As reported by Song et al. [133], an Nc based only on rice leaf dry matter was expressed as Nc = 1.96LDM −052 . The in-season crop N nutritional status can be evaluated as proposed by Chen et al. [134] for winter wheat based on three classes: (a) N deficient for NNI < 1.00, (b) N optimum for 1.00 ≤ NNI ≤ 1.25; (c) N surplus: NNI ≥ 1.25.
As discussed in Part 4, the yield is the outcome of the efficiency of crop production factors. A major characteristic of a crop plant is its time variability in requirements for water, nitrogen, and nutrients responsible for the efficiency of both key growth factors. The crop plant requirement for N f is the key in-season nutritional factor, responsible for the development and status of yield components. The N f dose can be calculated based on two characteristics of crop canopy: (1) the rate biomass increase during a particular phase, in particular, within the period defined by cardinal knots ( Figure 5); (2) the trend in N concentration in plants during vegetative growth, with special attention to CKs.
A sequential determination of the N content along the growing season of a plant is a primary tool for its N status assessment. As a rule, during the vegetative growth of the seed crop, the proportion of active metabolic tissues (leaves) decreases stage-by-stage with respect to structural ones (stem). Consequently, the total N concentration in plant tissues decreases during vegetative growth, but its content in the plant progressively increases, reaching the highest value just before the onset of flowering (CK2). The temporal variability in relationships between crop dry matter biomass and N concentration is described by the following set of equations [132]: (1) Nc-the critical N concentration, % or g N kg −1 DM N c = aW −b (20) (2) NNI-Nitrogen nutrition index, NNI = N a /N c (21) where: W-crop biomass, t ha −1 ; a and b-estimated parameters. The N c curve is expressed as a power function, but its parameters are both crop and growth condition specific. As reported by Song et al. [133], an N c based only on rice leaf dry matter was expressed as N c = 1.96L DM −052 . The in-season crop N nutritional status can be evaluated as proposed by Chen et al. [134] for winter wheat based on three classes: (a) N deficient for NNI < 1.00, (b) N optimum for 1.00 ≤ NNI ≤ 1.25; (c) N surplus: NNI ≥ 1.25.
As discussed in Part 4, the yield is the outcome of the efficiency of crop production factors. A major characteristic of a crop plant is its time variability in requirements for water, nitrogen, and nutrients responsible for the efficiency of both key growth factors. In-season evaluation of plant nutritional status relies on plant destructive sampling and nutrient concentration determination in the lab. The key disadvantages of classic diagnostic methods are: (1) ex-post data, delaying the diagnosis of plant nutritional status, and in consequence a recommendation of the N f rate; (2) lack of data about spatial heterogeneity in plant nutritional status, resulting in (a) uniform N fertilizer rate application, hardly related to the real, i.e., spatial variability in plant requirements for N, (b) low efficiency of applied nitrogen, A significant increase in NUE requires the implementation of new diagnostic tools, capable of quantifying a crop plant requirement for N in real-time (a defined stage of plant development) and taking into account spatial differences in the productivity of field homogenous units. There has been considerable technological progress in developing different sorts of non-invasive instruments of potential use in plant nutritional status determination [135][136][137]. Remote sensing is a technique that offers a broad set of effective diagnostic tools to meet both production and environmental objectives. All spectral techniques rely on the plant's ability to absorb and simultaneously reflect solar radiation. Remote-sensing techniques can make a rapid assessment of plant biomass, leaf area index, nitrogen content, chlorophyll content, and finally yield [138]. The information capacity of hyper-and multi-spectral imagery is several times larger as compared to any classical diagnostic tool.
Based on the spectral imagery of a field, it is possible to create a zonal map of a temporary crop N status. The selection of the most reliable spectral indices depends on the sensitivity of spectral bands to both the total N course during plant growth (Nitrogen Dilution Curve-N c ) and canopy structure (biomass, density, N concentration). It has been confirmed that the intensity of solar radiation reflectance in visible light (waveband 400 to 720 nm) is negatively correlated with leaf N content, while NIR reflectance is positively correlated with leaf N content and/or crop biomass [139]. The Normalized Difference Vegetation Index (NDVI) is the most frequently used diagnostic tool for the N content and crop biomass determination [140][141][142]. In fact, NDVI allows a reliable diagnosis of N status in cereals only during the early stages of growth. This index reaches saturation status at dense canopies [143,144]. This weakness has been recently overcome by developing a new set of Vegetable Spectral Indices (VIs) worked out on bands lying in other spectral regions, including the red-edge region (700-740 nm). Scientific reports have published data on VIs which are capable of predicting an LAI extending from 0 to 6 [145].

Conclusions
The sustainable intensification of agriculture is a concept which relies on the assumption of realizing two, seemingly contradictory goals, i.e., increasing food production but without a negative impact on local or global ecosystems. This twin objective can be achieved if solutions strictly oriented towards amelioration of the yield gap are put into agricultural practice, which will involve a reliance on increasing efficient nitrogen use. The inefficiency of nitrogen in the soil/crop plant system, i.e., the Nitrogen Gap, requires a three-dimensional diagnosis. The first, as a matter of fact, the major variability refers to the in-season variability in the nitrogen requirements of the currently grown crop. This gap can be filled by a synchronization of the demand of a grown plant for nutrients, in fact, driven by nitrogen applied in the required N f dose and at the right time. The recorded inefficiency of in-season applied nitrogen is due to the spatial variability of its supply. The main reason for the occurrence of the NG is both spatial and vertical variability in soil characteristics responsible for N uptake and its subsequent utilization by plants. The recognition of the size and strength of a plant's growth constraints, the disturbing formation of yield, is the prerequisite for dividing a field into zones of homogenous levels of productivity. Because the capacity of soil to soil water storage depends on the content of colloidal particles, the first goal of soil fertility amelioration is to increase the humus stock. This production objective is especially important for field zones naturally poor in mineral colloids. The subsequent steps in the action oriented to soil fertility amelioration depend on a farmer's ability to recognize the level of the subsoil fertility, including the content of available water and nutrients, and on the access of the currently grown crop to these resources during the growing season. A diagnosis of the soil fertility status is important for the development of application techniques of both nitrogen and nutrients, supporting its use efficiency. Classic and remote sensing techniques should be simultaneously applied to delineate the field into units of the homogenous level of productivity. Remote-sensing is the most efficient tool for the in-season diagnosis of the N plant status, provided it can discriminate the productivity of each field unit.

Conflicts of Interest:
The authors declare no conflict of interest.