Pomegranate Cultivation in Mediterranean Climate: Plant Adaptation and Fruit Quality of ‘Mollar de Elche’ and ‘Wonderful’ Cultivars

: Two pomegranate cultivars, Mollar de Elche, native to Spain, and Wonderful, the most widely cultivated pomegranate variety in the world, were studied in a commercial orchard in Sicily, and their phenological behavior was compared. During the development of the fruit, their dimensional growth was monitored, and after harvest, physicochemical and sensory analyses were carried out to highlight the differences between fruit of the two cultivars. Results showed that Mollar de Elche fruit require less time from ﬂowering to fruit maturity, resulting in an earlier harvest, which was due also to a higher relative growth rate of the fruit’s diameters. On the other hand, physicochemical analyses conﬁrmed that Wonderful is the variety that is most appealing for fresh produce consumers due to its intense coloring. However, Mollar de Elche showed economically desirable characteristics such as a good softness of the seed. Moreover, sensory analysis showed that fruit of the cv. Mollar de Elche was found to have a sweeter taste of both arils and juice.


Introduction
Pomegranate (Punica granatum L.) is an ancient deciduous tree crop that has been domesticated for thousands of years and offers a broad range of varieties and consumption opportunities for the consumers [1][2][3].
It is native to the region extending from Iran to the Himalayas in northern India, and Mediterranean and Middle-East countries are currently the main regions of pomegranate cultivation and production [4,5].
According to Levin [6], there are three large centers (primary, secondary, and tertiary) and five macro centers (Middle Eastern, Mediterranean, Eastern Asia, American, and South African) of origin and genetic diversity of the pomegranate. It is a temperate species that requires high summer temperatures to ripen properly. For this reason, its cultivation is relegated to the Mediterranean basin, southern Asia, and several countries of North and South America. Its adaptation to the Mediterranean climate has led to its wide diffusion and the creation of a multitude of new genetic individuals over time.
As a result of the low levels of respiration and ethylene products, the pomegranate is considered a non-climacteric fruit [7]. Pomegranate fruit maturity status is commonly assessed based on skin color and juice color and acidity [8]. The acceptability of pomegranate by the consumer depends on a combination of several quality attributes that are related to the physicochemical and mechanical properties including skin color, absence of physical defects, sugar content, acidity, and flavor [9]. Sweet and sweet-sour cultivars were liked better than sour cultivars, which can be described as having over 1.85% titratable acidity in citric acid equivalents (TA) at maturity. They also found that sweetness and sourness (Spain) based on the BBCH (Biologische Bundesanstalt, Bundessortenamt and Chemical industry) General Scale developed by Bleiholder, Van den Boom, Langeluddeke, and Stauss [36] but, as of today, no studies were conducted on cv. Wonderful.
The goal of this work was to monitor the phenological evolution of Wonderful pomegranate comparing with Mollar de Elche in the growing environment of South Italy (Sicily), according to the BBCH scale and assess the quality attributes of these pomegranate varieties, which represent an innovation in terms of physiochemical characteristics and sensory profile compared to traditional ones, in order to evaluate their attitude to fresh consumption and fresh-cut produce or juice transformation.

Materials and Methods
The trial was carried out in a commercial orchard located in the territory of Camastra, in the province of Agrigento, Sicily (37°15′04″ N-13°47′39″ E), consisting of 12-year-old trees. The BBCH pomegranate phenological scale, defined by Melgarejo et al. [24], was taken into consideration to evaluate the growth of the fruit of the two cultivars in relation to climatic data. The latter were supplied by SIAS, Sicilian Agro-meteorological Service, taking as reference the telemetering station "Mandrascava" (40 m above sea level) located in the vicinity of the orchard.
According to the data provided by the weather station, the yearly average temperature in this area is 23.6 °C, and the average yearly rainfalls are of 647 mm, with 78 rainy days. Under the bioclimatic aspect, the zone is referred as belonging to the upper infra-Mediterranean lower sub-humid bioclimatic belt [37].
The following parameters were monitored to describe the growth environment of the selected varieties: rainfall (mm), daily minimum and maximum relative humidity (%), daily minimum and maximum temperatures (°C) (Figure 1).
Data provided from SIAS showed that the period considered in this study  October 2014) was characterized by a reduced rainfall (only 4.8 mm in September) compared to the total 491.6 mm of the year, and an average temperature of 28.5 °C.

Plant Material
Fruit were harvested on different dates depending on the ripening times of the two varieties. Fruit of the cv. Mollar de Elche, the earliest variety, were harvested in the first decade of October, whereas fruit of the cv. Wonderful were picked in the third decade of October. The fruit were harvested at commercial maturity using the total soluble solids content (TSS)/TA ratio as harvest index [38]. The plants were trained to a vase shape, and both cultivars receive the same amount of inputs.

Experimental Design
Fifteen trees were selected for each variety, and the phenological stages were monitored for each plant, based on the pomegranate BBCH scale [34]. Ten fruit per plant were labeled, and their growth was evaluated by measuring the transverse diameter TD (mm) Data provided from SIAS showed that the period considered in this study (30 June-28 October 2014) was characterized by a reduced rainfall (only 4.8 mm in September) compared to the total 491.6 mm of the year, and an average temperature of 28.5 • C.

Plant Material
Fruit were harvested on different dates depending on the ripening times of the two varieties. Fruit of the cv. Mollar de Elche, the earliest variety, were harvested in the first decade of October, whereas fruit of the cv. Wonderful were picked in the third decade of October. The fruit were harvested at commercial maturity using the total soluble solids content (TSS)/TA ratio as harvest index [38]. The plants were trained to a vase shape, and both cultivars receive the same amount of inputs.

Experimental Design
Fifteen trees were selected for each variety, and the phenological stages were monitored for each plant, based on the pomegranate BBCH scale [34]. Ten fruit per plant were labeled, and their growth was evaluated by measuring the transverse diameter TD (mm) and the longitudinal diameter LD (mm) at two-week intervals using a digital caliper Agronomy 2021, 11, 156 4 of 16 (TR53307, Turoni, Forlì, Italy), for a total of seven measurements over a three-month long period from July to October.
The relative growth rate (RGR; %) of the two diameters was obtained calculating the percentage variation of each reading compared to the previous one.
The Shape Index (SI; %) of the fruit, which is a measure of their sphericity, was calculated as LD/TD. The volumetric growth of the fruit throughout their development was also estimated, assuming the shape as spherical, calculating their volume as where r was obtained as the half of the mean of the two diameters [39]. When ripening point was reached, the fruit were harvested and immediately submitted to the postharvest laboratory for physicochemical and sensory analysis. The same 10 fruit for each plant of both varieties were harvested for each plant, 100 of them were used for physical-chemical analysis and 50 were used for sensory analysis.

Physicochemical Analyses
For each fruit, length without calyx (longitudinal diameter-LD; mm) and equatorial width (transverse diameter-TD; mm) were measured with a digital caliper (TR53307, Turoni, Forlì, Italy). Fruit weight (FW) was measured with a digital scale with an accuracy to two decimal places (Gibertini EU-C 2002 RS, Novate Milanese, Italy).
For the color evaluation of fruit skin and the arils of each fruit, both were photographed with a DSLR camera (Figures 2 and 3) and then the F.A.S. (Fruit Analysis System) software, based on a MATLAB 6.0 algorithm that converts images from the RGB format to the CIE format (L* = lightness, a* = green (−100) to red (+100), b* = blue (−100) to yellow (+100)) [29], was used on the photos. Then, it was possible to obtain Fruit Color Index (FCI) and Arils Color Index (ICA), which were calculated as the Color Index (CI) (using Equation (1)) [40] for the fruit skin and arils, respectively: Agronomy 2021, 11, × 4 of 16 and the longitudinal diameter LD (mm) at two-week intervals using a digital caliper (TR53307, Turoni, Forlì, Italy), for a total of seven measurements over a three-month long period from July to October. The relative growth rate (RGR; %) of the two diameters was obtained calculating the percentage variation of each reading compared to the previous one.
The Shape Index (SI; %) of the fruit, which is a measure of their sphericity, was calculated as LD/TD. The volumetric growth of the fruit throughout their development was also estimated, assuming the shape as spherical, calculating their volume as 4 3 where r was obtained as the half of the mean of the two diameters [39]. When ripening point was reached, the fruit were harvested and immediately submitted to the postharvest laboratory for physicochemical and sensory analysis. The same 10 fruit for each plant of both varieties were harvested for each plant, 100 of them were used for physical-chemical analysis and 50 were used for sensory analysis.

Physicochemical Analyses
For each fruit, length without calyx (longitudinal diameter-LD; mm) and equatorial width (transverse diameter-TD; mm) were measured with a digital caliper (TR53307, Turoni, Forlì, Italy). Fruit weight (FW) was measured with a digital scale with an accuracy to two decimal places (Gibertini EU-C 2002 RS, Novate Milanese, Italy).
For the color evaluation of fruit skin and the arils of each fruit, both were photographed with a DSLR camera (Figures 2 and 3) and then the F.A.S. (Fruit Analysis System) software, based on a MATLAB 6.0 algorithm that converts images from the RGB format to the CIE format (L* = lightness, a* = green (−100) to red (+100), b* = blue (−100) to yellow (+100)) [29], was used on the photos. Then, it was possible to obtain Fruit Color Index (FCI) and Arils Color Index (ICA), which were calculated as the Color Index (CI) (using Equation (1)) [40] for the fruit skin and arils, respectively: * * * .
(1)   The pixel ratio between the red-colored area and total fruit area was used to quantify the percentage of cover color (CC%) [41].
After the non-destructive analyses, the same fruit were opened with a curved knife, and the following determinations were carried out on each fruit: number of arils (NA);  The pixel ratio between the red-colored area and total fruit area was used to quantify the percentage of cover color (CC%) [41].
After the non-destructive analyses, the same fruit were opened with a curved knife, and the following determinations were carried out on each fruit: number of arils (NA); arils weight (WA; g); skin weight (SW = FW − WA; g); skin thickness (ST; mm); skin index (SkI = SW/FW × 100); skin thickness index (STI = ST/TD × 100); weight of 20 arils (WA 20 ;g); arils height (AH; mm); arils width (AW; mm); arils yield (AY = FW − PW); weight of 20 seeds (SdW 20 ; g); arils colour (ICA) and woody part index (WPI = SdW/WA × 100). Traits of the arils such as AH and AW were obtained as the means of readings on samples of 40 arils A digital caliper (TR53307, Turoni, Forlì, Italy) was used for diameter and thickness measurements, and weight of the fruit and arils was determined using an electronic platform scale (Gibertini EU-C 2002 RS, Novate Milanese, Italy).
The juice of each fruit was obtained with a centrifugal juicer (Centrika Metal, Mod. 0173, Ariete, Italy) to obtain juice on which the following parameters were determined: juice content (JC; mL/100 g) determined with a graduated cylinder (500 mL), total soluble solids content (TSS; • Brix) determined using an Atago Pal-1 pocket refractometer (Atago Co., Ltd., Tokyo, Japan), titratable acidity (TA; g citric acid/L) determined with a Crison Compact titrator pH-meter (Crison Instruments, SA, Barcelona, Spain) with 0.1 N NaOH up to pH 8.1, and juice color index (JCI) by photographing the juice and then using the F.A.S. software (Figures 4 and 5). The pixel ratio between the red-colored area and total fruit area was used to quantify the percentage of cover color (CC%) [41].
The juice of each fruit was obtained with a centrifugal juicer (Centrika Metal, Mod. 0173, Ariete, Italy) to obtain juice on which the following parameters were determined: juice content (JC; mL/100 g) determined with a graduated cylinder (500 mL), total soluble solids content (TSS; °Brix) determined using an Atago Pal-1 pocket refractometer (Atago Co., Ltd., Tokyo, Japan), titratable acidity (TA; g citric acid/L) determined with a Crison Compact titrator pH-meter (Crison Instruments, SA, Barcelona, Spain) with 0.1 N NaOH up to pH 8.1, and juice color index (JCI) by photographing the juice and then using the F.A.S. software (Figures 4 and 5).   The pixel ratio between the red-colored area and total fruit area was used to quantify the percentage of cover color (CC%) [41].
The juice of each fruit was obtained with a centrifugal juicer (Centrika Metal, Mod. 0173, Ariete, Italy) to obtain juice on which the following parameters were determined: juice content (JC; mL/100 g) determined with a graduated cylinder (500 mL), total soluble solids content (TSS; °Brix) determined using an Atago Pal-1 pocket refractometer (Atago Co., Ltd., Tokyo, Japan), titratable acidity (TA; g citric acid/L) determined with a Crison Compact titrator pH-meter (Crison Instruments, SA, Barcelona, Spain) with 0.1 N NaOH up to pH 8.1, and juice color index (JCI) by photographing the juice and then using the F.A.S. software (Figures 4 and 5).

Sensory Analysis
Twenty-five fruit of each variety were used to collect respectively 1500 g of arils, which were later subjected to evaluation by a panel of semi-experienced members. Twenty-five more fruit of each variety were used to collect 1000 mL of arils juice of each variety, on which a parallel sensory analysis was carried out on a single session.
So, each judge was provided with fifteen arils, which were divided in three replicates of five arils (for a total of about 40 g), of each variety and, later, with a sample of 40 mL of arils juice, divided in two replicates of 20 mL, of each variety.
The sensory profile (assessed according to UNI 10957:2003 legislation) was defined by a group of 30 judges, who were previously trained with four training sessions in the recognition of qualitative characteristics; they generated 11 descriptors for arils and 10 descriptors for juice at a preliminary meeting [20,[42][43][44][45]. The following descriptors have been identified on the arils on the basis of the citation frequency (>60%) and listed below: two for the tactile ones (easy peeling and easy separation of the arils), one for the sight (color), two for the odor (odor of pomegranate and herbaceous), four for the taste (acid, sweet, bitter, astringent), and two for the flavor (flavor of pomegranate and herbaceous).
For the juice, the following descriptors were chosen: one for the sight (color), one for the rheology (density), two for the odor (odor of pomegranate and herbaceous), four for the taste (acid, sweet, bitter, astringent), and two for the flavor (flavor of pomegranate and herbaceous) The judges evaluated the intensity of each attribute by assigning a score from 1 (no sensation) to 9 (maximum sensation intensity). The evaluation for arils and the one for juice were carried out at the sensory analysis laboratory, which was built according to UNI ISO 8589 legislation, equipped with a specific software for sensory data acquisition (FIZZ Biosystemes, Couternon, France). The assessments were carried out between 10 am and 12 pm in individual cabins. The order in which samples were presented was randomized between judges and sessions. Water was provided between samples to rinse the mouth. Thus, the obtained profile allowed quantifying the characteristics of the samples individually and in order of perception [46].

Statistical Analysis
Analysis of variance was applied to all the variables studied. The mean values obtained in the different categories were compared by one-way ANOVA, and significant differences among means at p < 0.05 were determined by Tukey's test. All statistical analysis was conducted using SigmaPlot software version 12.0 (Systat Software, Inc., San Jose, CA, USA).

Phenological Stages Evolution
Based on the BBCH scale by Melgarejo et al. [34], it was possible to keep track of the phenological evolution of the cv. Mollar de Elche and cv. Wonderful, cultivated in Sicily.
The prevailing phenological stages of the two cultivars are shown in the Figure 6. Both varieties stay in the same phenological stage, leaves growth stage (D3:11), from January to the first decade of April. Both varieties showed a similar initial growth pattern, and instead, differences in the progress of phenological phases were found from the first decade of July to the first decade of September due to different ripening periods, as was seen in the work of Ikinci et al. [35].
In fact, it can be seen that flowers in Mollar de Elche appear (phenological stage E: appearance of the flower bud and calix) already at the end of April, while Wonderful reaches the same stage only at the middle of May.
Fruit of the cv. Mollar de Elche also require a shorter period to complete their growing: in fact, they enter the phenological stage "L" of fruit ripening on the 10th of September, and harvest begins on the 10th of October.
Instead, cv. Wonderful enters the same stage on the 20th of August and harvest only begins on the 30th of October. These results reflect what has been observed in various other cultivation areas [2], and this implies that fruit of cv. Mollar de Elche can be placed on the fresh fruit market two weeks ahead of those of the cv. Wonderful, giving growers the opportunity to obtain a more rewarding selling price. The phenological stages of fruit set and fruit growth happen during the hot and dry summer season. During this time of the year, temperatures are favorable to the fruit growth [35], but at the same time, the water requirements of the trees are the highest. Therefore, it can be stated that the cultivation of pomegranate in Sicily cannot prescind from the use of irrigation, which also plays a fundamental role in obtaining quality fruit [2,47,48]. Both varieties showed a similar initial growth pattern, and instead, differences in the progress of phenological phases were found from the first decade of July to the first decade of September due to different ripening periods, as was seen in the work of Ikinci et al. [35].

Fruit Dimensional Growth
In fact, it can be seen that flowers in Mollar de Elche appear (phenological stage E: appearance of the flower bud and calix) already at the end of April, while Wonderful reaches the same stage only at the middle of May.
Fruit of the cv. Mollar de Elche also require a shorter period to complete their growing: in fact, they enter the phenological stage "L" of fruit ripening on the 10th of September, and harvest begins on the 10th of October.
Instead, cv. Wonderful enters the same stage on the 20th of August and harvest only begins on the 30th of October. These results reflect what has been observed in various other cultivation areas [2], and this implies that fruit of cv. Mollar de Elche can be placed on the fresh fruit market two weeks ahead of those of the cv. Wonderful, giving growers the opportunity to obtain a more rewarding selling price.
The phenological stages of fruit set and fruit growth happen during the hot and dry summer season. During this time of the year, temperatures are favorable to the fruit growth [35], but at the same time, the water requirements of the trees are the highest. Therefore, it can be stated that the cultivation of pomegranate in Sicily cannot prescind from the use of irrigation, which also plays a fundamental role in obtaining quality fruit [2,47,48].

Fruit Dimensional Growth
Fruit growth, measured by means of the transversal and longitudinal diameters, is very similar between the two cultivars ( Figure 7); both cultivars show the sigmoidal pattern typical of the species, described by Gozlekci and Kaynak [49]. During the first ten days of August, Wonderful grew in width by 4.98 mm and in height by 4.69 mm, and those of Mollar de Elche grew by 8.93 and 7.12 mm, respectively. This difference emphasizes the different phenological phases of the two cultivars: cv. Wonderful is in the second germination phase (K:39) and cv. Mollar de Elche is in the previous phase, which is the growth of the fruit (J:73); for cv. Wonderful in this phase continues until the second decade of August.
In August, there was a slowdown in fruit growth for both cultivars, which is graphically reflected in the inflection of the characteristic curve ( Figure 7)-a trend certainly influenced by high temperatures [50]. The slowdown in growth continued until the first decade of September, although the two varieties were at the stage of ripeness of the fruit (L:81,85); in fact, in the second decade, the growth of cv. Mollar de Elche is 7.36 mm, and it is 10.19 mm in cv. Wonderful. This increase in growth is certainly favored by the increase in irrigation volumes, which are used to facilitate the rapid growth of the fruit.
During the whole period of the study, the relative growth rate (RGR) of the two diameters of the fruit showed a very similar trend in both cultivars. A peak in the percentage of growth rate can be seen in the first period of fruit growth, which is then followed by a strong decrease that can be attributed to the rise in the temperatures, as shown in Figure  1.
Then, following the beginning of irrigation in the second half of August, the RGR values go back to average values between 10 and 15%, until fruit reach their final dimensions. It is interesting to notice how, despite cv. Mollar de Elche being the smallest variety, RGR values reach a higher peak (35%) in this cv., while the peak of relative growth for cv. Wonderful is 26%.
The SI (%) of the two cultivars shows that the young fruit (phenological stage I:71) of both cultivars has a slightly elongated shape. Fruit of the cv. Wonderful later assume an almost perfect spherical shape until the end of its growth (phenological stages L:81, L:85), with an SI value of 0.987%, while fruit of the cv. Mollar de Elche appear to be slightly more swollen at the moment of the harvest with an SI of 0.931% (Figure 8).
The volume (cm 3 ) of the fruit showed a similar increase trend in the two cultivars during the observation period, and the fruit of the cv. Wonderful turned out to be the biggest ones with an average volume of 56.214 cm 3 at the end of their growth, while the fruit of the cv. Mollar de Elche had a lower average volume of 37.991 cm 3 before harvest ( Figure 9).  In the second decade of July, the value for the Wonderful is 66.71 mm width in contrast to 52.19 mm for Mollar de Elche. Notwithstanding, this indicates that the fruit of the two cv. have grown almost identically, respectively 13.72 and 13.59 mm.
During the first ten days of August, Wonderful grew in width by 4.98 mm and in height by 4.69 mm, and those of Mollar de Elche grew by 8.93 and 7.12 mm, respectively. This difference emphasizes the different phenological phases of the two cultivars: cv. Wonderful is in the second germination phase (K:39) and cv. Mollar de Elche is in the previous phase, which is the growth of the fruit (J:73); for cv. Wonderful in this phase continues until the second decade of August.
In August, there was a slowdown in fruit growth for both cultivars, which is graphically reflected in the inflection of the characteristic curve ( Figure 7)-a trend certainly influenced by high temperatures [50]. The slowdown in growth continued until the first decade of September, although the two varieties were at the stage of ripeness of the fruit (L:81,85); in fact, in the second decade, the growth of cv. Mollar de Elche is 7.36 mm, and it is 10.19 mm in cv. Wonderful. This increase in growth is certainly favored by the increase in irrigation volumes, which are used to facilitate the rapid growth of the fruit.
During the whole period of the study, the relative growth rate (RGR) of the two diameters of the fruit showed a very similar trend in both cultivars. A peak in the percentage of growth rate can be seen in the first period of fruit growth, which is then followed by a strong decrease that can be attributed to the rise in the temperatures, as shown in Figure 1.
Then, following the beginning of irrigation in the second half of August, the RGR values go back to average values between 10 and 15%, until fruit reach their final dimensions. It is interesting to notice how, despite cv. Mollar de Elche being the smallest variety, RGR values reach a higher peak (35%) in this cv., while the peak of relative growth for cv. Wonderful is 26%.
The SI (%) of the two cultivars shows that the young fruit (phenological stage I:71) of both cultivars has a slightly elongated shape. Fruit of the cv. Wonderful later assume an almost perfect spherical shape until the end of its growth (phenological stages L:81, L:85), with an SI value of 0.987%, while fruit of the cv. Mollar de Elche appear to be slightly more swollen at the moment of the harvest with an SI of 0.931% (Figure 8).      Table 1 shows the external appearance characteristics of the fruit of the cv. Wonderful and cv. Mollar de Elche. During the development and ripening of the fruit of the Spanish cv. Mollar de Elche, the skin color changes ranging from green to greenish yellow and, finally, to brownish yellow with reddish spots (data not shown) [34]. Several studies on the cv. Wonderful cultivar have shown that the red pigmentation increases significantly during ripening [51].

Physicochemical Analyses
The FCI is higher in cv. Wonderful, which indicates a more intense red color than cv. Mollar de Elche. Another important parameter is the percentage of CC (%). The consumer is more attracted to fruit with a more intense deep red tint and uniform CC [52,53].
The color characteristics of pomegranate cultivars also indicate the possibility of using these values as a ripening index and for the separation of the fruit into commercial categories [54].

Cultivar
FCI CC (%) Wonderful 0.97 ± 0.01 a 100 ± 0.01 a Mollar de Elche 0.94 ± 0.01 a 59 ± 1.10 b Table 2 shows the skin characteristics of the two cv. analyzed. It is immediately noticeable that the fruit of the cv. Wonderful have a greater size and weight and can be classified in the UNECE (United Nations Economic Commission for Europe) category 1/A, while cv. Mollar de Elche fruit fall into category 2/B [52].
ST was 4.44 mm in cv. Wonderful and 4.24 mm in cv. Mollar de Elche. This parameter can be interesting for post-harvest management; in fact, a greater thickness of the skin decreases the transpiration of the fruit during storage [55].  Table 1 shows the external appearance characteristics of the fruit of the cv. Wonderful and cv. Mollar de Elche. During the development and ripening of the fruit of the Spanish cv. Mollar de Elche, the skin color changes ranging from green to greenish yellow and, finally, to brownish yellow with reddish spots (data not shown) [34]. Several studies on the cv. Wonderful cultivar have shown that the red pigmentation increases significantly during ripening [51]. The FCI is higher in cv. Wonderful, which indicates a more intense red color than cv. Mollar de Elche. Another important parameter is the percentage of CC (%). The consumer is more attracted to fruit with a more intense deep red tint and uniform CC [52,53].

Physicochemical Analyses
The color characteristics of pomegranate cultivars also indicate the possibility of using these values as a ripening index and for the separation of the fruit into commercial categories [54]. Table 2 shows the skin characteristics of the two cv. analyzed. It is immediately noticeable that the fruit of the cv. Wonderful have a greater size and weight and can be classified in the UNECE (United Nations Economic Commission for Europe) category 1/A, while cv. Mollar de Elche fruit fall into category 2/B [52]. ST was 4.44 mm in cv. Wonderful and 4.24 mm in cv. Mollar de Elche. This parameter can be interesting for post-harvest management; in fact, a greater thickness of the skin decreases the transpiration of the fruit during storage [55].
SkI indicates the incidence of the skin on the total weight of the fruit and, it can be noticed that the cv. Mollar de Elche has a higher SkI than the cv. Wonderful. This parameter could be useful in order to be able to classify the fruit commercially, with particular importance in the processing sector, since, for the same weight of fruit, the market prefers fruit with the highest edible part yield [46]. In the cv. Mollar de Elche, both ST and STI values are similar to those determined by Hernández et al. [56].
The parameters measured on arils determine their economic value and thus represent important factors for growers, the market, and industry [56]. Tables 3 and 4 show the quality characteristics respectively of the arils and seeds.  As regards arils (Table 3), NA varies considerably between the two cultivars, cv. Wonderful has the highest number of arils and a higher yield of arils (YA). This parameter indicates the incidence of the weight of arils on the fruit fresh weight: a higher yield in arils reflects in a better commercial quality of the fruit [50].
There is no correlation between skin color and aril color within the same fruit [31]; the two varieties reached a very high and similar value of ICA, demonstrating no significant difference for this parameter.
The WA 20 is higher in cv. Mollar de Elche: this value is correlated to a higher HA and LA, which are values that are in agreement with the examinations carried out by Hernández et al. [56].
Seeds softness is a desirable economic trait that enhances the consumptive qualities of fruit [57], so WPI and SdW 20 were measured on the fruit (Table 4). WPI is considered to be of minor importance in relation to the raw fiber content and firmness of the seed, but it gives a good idea of the low palatability of the seed because it refers to the quantity of ligneous material in relation to the total weight of the seed [58].
Both indices turned out to be smaller in the Mollar de Elche variety, and according to Mena et al. [59], this may make this variety more suitable for fresh consumption. Table 5 shows all the physicochemical characteristics determined on the juice. JC (mL per 100 g) does not significantly vary between the two varieties. Both varieties have reported values that satisfy the requirements for the juice extraction industry. This parameter is of great interest for the industry, but also for the consumer, due to the nutraceutical value of the juice [23,60]. Melgarejo et al. [22] classified pomegranate cultivars into three levels of sweetness, depending on their soluble solids content (TSS) and titratable acidity (TA): the varieties were divided into "sweet" (TSS levels above 13 and TA below 0.7%), "sweet and acid" (TSS 12-13% and TA 0.7-1.8%), and "acid" (TSS below 12% and TA above 1.8%). According to Chace et al. [61], in fact, pomegranates are appropriate for the fresh market when their acidity content is less than 1.8%. These values are not stable but vary during maturation and the post-harvest phase [22].
As for the TSS content of the juice, the two cv. do not differ significantly: in cv. Wonderful, this parameter scored 16.74 • Brix, and 16.14 • Brix in the cv. Mollar de Elche, which are values that are in line with the work of Andreu-Sevilla et al. [62]. We can say that based on the physical and chemical characteristics present in the juice, the cultivar Mollar de Elche is more oriented toward the production of juices, and as confirmed by other studies [59].
Another important characteristic to take into account in pomegranate is the ratio of TSS/TA [63], which is a more reliable indicator of taste, quality, and ripeness than TSS alone. In cv. Mollar de Elche, the TSS/TA ratio has a value of 16.14-higher than cv. Wonderful, which has a value of 11.16. This parameter is also used as a destructive index to assess the degree of ripeness of the fruit and, therefore, the optimal time for harvesting.
To date, the following classification has been used for Spanish varieties based on TSS/TA value [12]: sweet varieties: 31-98; sweet-sour varieties: 17-24; acid varieties: 5-7. Both varieties, according to the above classification, fall between the groups of sweet-sour and acid varieties. However, referring to the Melgarejo classification [12], the fruit of both cultivars are identified as sweet-sour. Figure 10a shows the results of the sensory analysis on arils. The descriptors that reported statistically significant differences are color, sweet, acid, and astringent. The judgment on the color of the arils was significantly higher in cv. Wonderful (8.22) than in the cv. Mollar de Elche (4.22), indicating a more intense coloring in the first cultivar. These sensory results are confirmed by colorimetric analyses, where cv. Mollar de Elche reported values lower than cv. Wonderful.

Sensory Analysis
Both the sweet and the acidic descriptor confirmed cv. Wonderful as the less sugary variety; in fact, the judges assigned values of 6.77 for the descriptor sweet and 2.44 for the descriptor acid in the cv. Mollar de Elche, and 3.22 for the descriptor sweet and 5.33 for descriptor acid in cv. Wonderful. This is in line with the TSS and TA values found and their ratio, which would indicate cv. Mollar de Elche as the sweetest.
It should be noticed that sweet varieties are certainly more appreciated for fresh consumption [10,58]. Descriptor astringency was higher in cv. Wonderful (6.00), while it was statistically lower in cv. Mollar de Elche (2.66).
Although the arils in the overall evaluation reached a score of 5.77 for cv. Wonderful and 6.55 for cv. Mollar de Elche, the differences are not statistically significant. Considering the significant descriptors, we were able to identify in cv. Mollar de Elche a higher suitability for fresh consumption, due to the higher value of sweetness, pomegranate odor, but also a lower sensation of acidity and astringency. The cv. Wonderful, with its high color intensity and lower pH value, seems to be more suitable for use as fresh-cut product [10].
Wonderful cv. gets a score of 4.77, while cv. Mollar de Elche gets a score of 7.00. Therefore, from the results obtained, we can conclude that the juice of the cv. Mollar de Elche is much sweeter, less acidic, and astringent, which are properties that allow a greater appreciation by the consumer, who could use this fruit to obtain juices to be consumed in a short time. On the other hand, Wonderful cv. shows less sweetness, greater acidity, astringency, and a much higher intensity than the red color, which suggests a variety that is more suitable for industrial use.  Figure 10b shows the sensory analysis on aril juice. The descriptors that showed statistically significant differences are color, herbaceous odor, sweet, acid and astringent. The juice color was assessed higher for the cv. Wonderful (7.66) than for the cv. Mollar de Elche (5.88). Similar values were found by Andreu-Sevilla et al. [62] who, comparing the pomegranate juice and the wine obtained from it, stated that a positive aspect of the Wonderful variety was its intense red color, which led to an intense color of the wine, while cv. Mollar de Elche had a juice with a color intensity of 3.5 ± 0.3. In addition, the herbaceous odor descriptor, which is usually linked to a more backward stage of maturation, is unfavorable in the cv. Wonderful because it is more perceived by the judges than in the cv. Mollar de Elche.
The descriptor sweetness score was lower in Wonderful (3.11) and higher in Mollar de Elche (7.66). For the acid descriptor, the scores confirm the results from the physicochemical analyses. The juice of the cv. Mollar de Elche, due to its lower acidity and pH and higher TSS content, is more suitable for immediate consumption; in fact, consumers prefer sweeter flavors to acidic ones [10]. The data obtained coincide with the results of Andreu-Sevilla et al. [62], who defines the juice of the cv. Wonderful as acid, astringent, with notes of vinegar and only slightly sweet, while the juice of the cv. Mollar de Elche is described as sweet, acid, and only slightly astringent.
The overall score also differs statistically significantly between the two cultivars, due to the lower acidity and pH value of the cv. Mollar de Elche and the higher TSS content. Wonderful cv. gets a score of 4.77, while cv. Mollar de Elche gets a score of 7.00. Therefore, from the results obtained, we can conclude that the juice of the cv. Mollar de Elche is much sweeter, less acidic, and astringent, which are properties that allow a greater appreciation by the consumer, who could use this fruit to obtain juices to be consumed in a short time. On the other hand, Wonderful cv. shows less sweetness, greater acidity, astringency, and a much higher intensity than the red color, which suggests a variety that is more suitable for industrial use.

Discussion
In this work, we showed that cv. Mollar de Elche can be considered an early variety compared to the market standard cv. Wonderful, as ripening and harvesting took place in the first ten days of October. The growth of the fruit was influenced by the high temperatures of the summer months, which made it essential to use basic irrigation, especially during the fruit's swelling period (from the end of August to the beginning of October) to support the processes of accumulation of bioactive connections and obtain high-quality fruit.
The results of pomological and internal physicochemical composition analyses confirm that it is possible, in Sicily, to obtain quality characteristics equal to those demonstrated by studies carried out on Wonderful and Mollar de Elche varieties in other suitable environments.
The fruit of the cv. Wonderful, with a more intense color and a homogeneous cover color, would seem to capture the consumer's attention more. However, the physicochemical properties of the juice indicate a good propensity to industrial production and to the marketing of the arils as fresh cut product. Mollar de Elche cv., with smaller size and less intense coloring is less attractive to the consumer, although the lower woody part index and the high TSS/TA ratio make it suitable to meet the consumer's needs for fresh fruit.
However, the results on the pomological characteristics and chemical composition of the varieties Wonderful and Mollar de Elche do not exclude the possibility that the two cultivars may be used for both fresh and processed fruit.
Sensory evaluations, on the other hand, while confirming the data from physicochemical analyses, seem to define more precisely the attitudes of both varieties. The juice of the cv. Wonderful has a stronger color, acidity, astringency, and herbaceous odor than the cv. Mollar de Elche, which are properties that make it preferable for juice production. Mollar de Elche's arils scored higher in the descriptors of the sweet, and lower in those of bitter, astringency, which are the properties that most affect the consumer of fresh fruit.
Further research should be carried out in order to better assess the cultivation of pomegranate in Sicily from a vegetative-productive and qualitative point of view. This would enable growers to better understand the influence of climatic factors during fruit development and ripening in order to improve the organoleptic and commercial quality of the fruit.
The results of this study on plant phenology, fruit growth, physicochemical characteristics, and sensory properties, which diversify the two cultivars, allowed us to identify the greatest potential of their cultivation in a Mediterranean climate, their influence on the market, and appreciation by the pomegranate consumer.