Influence of Detasseling Methods on Seed Yield of Some Parent Inbred Lines of Turda Maize Hybrids

During the process of maize seed production, in order to ensure the genetic purity of parental forms of hybrid maize, an important work performed is the removal of male inflorescences from plants on mother rows. Hand detasseling has high precision but is labor-intensive. Mechanical detasseling offers the possibility to cover large acreages in a short period of time, but the number of leaves removed has a varying influence on plant performance and seed yield. The aim of this study was to simulate three types of damages on plants similar to those induced through mechanical detasseling and to assess the effects for five inbred lines during the course of three years. Results show that when tassels alone were removed, the average seed yield decreased an average of 4–21%. When two leaves were removed with the tassel, yield decreased an average of 22–31%, while when plants were cut above the main ear, seed yield decreased an average of 31–66%. Environmental conditions influenced seed yield, especially high temperatures during flowering. Yield response to tassel and leaves removal varied between the inbred lines. Genotype controls maize ear and kernel characters, while environmental factors exercise a strong influence on seed yield, due to the succession of years with contrasting weather conditions in a key phenophase. Within the trend of full mechanization in agriculture, identification of inbred lines that cope better with plant damage can assist in optimizing seed production.


Introduction
Zea mays L. is one of the most cultivated cereal crops in the world. It is used in the human diet, as animal feed, and to the obtaining of many other industrial products [1][2][3][4]. In Romania, maize was brought about 300 years ago and gradually replaced old millet plantations until becoming a staple food [2]. According to the Ministry of Agriculture and Rural Development of Romania, in less than 10 years after joining the European Union (EU), national maize production doubled [5]. Today, Romania is recognized as the largest maize grower within the EU [6,7]. Because domestic maize requirements are only around 5 million tons while production exceeds 10 million tones, there is significant export potential [7]. To capitalize on this opportunity, maize breeders face the complex challenge to increase the cost efficiency of maize seed production, minimize seed losses, and ensure an increasing supply of high-quality seed. Currently, at the national level, breeding efforts and testing of new maize genotypes are conducted by research stations, growers' associations and companies established in different regions of the country [8]. Important maize germplasm collections used in breeding programs can be found at several research stations across Romania, such as the Agricultural Research and Development Station from Turda (ARDS) [9].
In the last six decades, most commercial maize crops use hybrid seed created based on inbred lines which are obtained through successive generations of self-pollination and artificial selection [10][11][12][13][14][15][16]. Given current importance maize has in Romania, optimization of the seed production process is incredibly important. To achieve this, besides specific technical measures meant to ensure the genetic purity of inbred lines [17][18][19], it is also important to study the behavior of inbred lines under different aspects and adapt seed production technology to their needs. During the seed production process based on male-fertile parental forms, the most important work performed is the detasseling of maize lots, which consists of removing tassels-the male maize inflorescences-from all plants on mother rows before shedding pollen. The degree of accuracy of detasseling directly influences the quality of seed obtained [17,20,21]. Through mechanical detasseling, costs can be reduced by about 30%, compared to manual detasseling [10]. However, the mechanical action of the detasseler that pulls tassels also damages the plant and leaves to varying degrees [20,22]. Due to the importance detasseling has in obtaining maize seed, there is a high interest in studying the influence of detasseling on corn performance, with a focus on mature grain yield [23]. This research is meant to bring a contribution to the understanding of factors affecting seed yield of maize inbred lines under the continental climate of Transylvania, with the purpose to identify possibilities to optimize certain stages of seed production.
The aim of this study was to assess the influence of different detasseling methods on the quantity and quality of seed yield, ear, and kernel characters for some inbred lines in pedo-climatic conditions of ARDS Turda, Romania during 2015-2017. Two objectives were defined: • evaluation of genotype response to three different plant damages, (similar to those induced by mechanical detasseling) in relation to several quantitative and qualitative traits; • identification of efficiency and suitability of this practice for tested inbred lines and make recommendations based on the link identified between experimental years, genotype, and detasseling method in local conditions.

Materials and Methods
The experiment was established at the Agricultural Research and Development Station (ARDS) from Turda, Romania, located at 46 • 34 50.5" N 23 • 47 16.4" E and took place between 2015-2017. Climate is temperate continental [24]. The field had clay-loam soil type, with good nitrogen, phosphorus, potassium and micronutrient content, as well as an average humus level. Topsoil had neutral pH [25]. Temperatures and precipitation levels during the experimental period are presented in Figure 1a,b.
Agronomy 2020, 10, x FOR PEER REVIEW 2 of 10 of new maize genotypes are conducted by research stations, growers' associations and companies established in different regions of the country [8]. Important maize germplasm collections used in breeding programs can be found at several research stations across Romania, such as the Agricultural Research and Development Station from Turda (ARDS) [9]. In the last six decades, most commercial maize crops use hybrid seed created based on inbred lines which are obtained through successive generations of self-pollination and artificial selection [10][11][12][13][14][15][16]. Given current importance maize has in Romania, optimization of the seed production process is incredibly important. To achieve this, besides specific technical measures meant to ensure the genetic purity of inbred lines [17][18][19], it is also important to study the behavior of inbred lines under different aspects and adapt seed production technology to their needs. During the seed production process based on male-fertile parental forms, the most important work performed is the detasseling of maize lots, which consists of removing tassels-the male maize inflorescences-from all plants on mother rows before shedding pollen. The degree of accuracy of detasseling directly influences the quality of seed obtained [17,20,21]. Through mechanical detasseling, costs can be reduced by about 30%, compared to manual detasseling [10]. However, the mechanical action of the detasseler that pulls tassels also damages the plant and leaves to varying degrees [20,22]. Due to the importance detasseling has in obtaining maize seed, there is a high interest in studying the influence of detasseling on corn performance, with a focus on mature grain yield [23]. This research is meant to bring a contribution to the understanding of factors affecting seed yield of maize inbred lines under the continental climate of Transylvania, with the purpose to identify possibilities to optimize certain stages of seed production.
The aim of this study was to assess the influence of different detasseling methods on the quantity and quality of seed yield, ear, and kernel characters for some inbred lines in pedo-climatic conditions of ARDS Turda, Romania during 2015-2017. Two objectives were defined: • evaluation of genotype response to three different plant damages, (similar to those induced by mechanical detasseling) in relation to several quantitative and qualitative traits; • identification of efficiency and suitability of this practice for tested inbred lines and make recommendations based on the link identified between experimental years, genotype, and detasseling method in local conditions.

Materials and Methods
The experiment was established at the Agricultural Research and Development Station (ARDS) from Turda, Romania, located at 46°34'50.5″ N 23°47'16.4″ E and took place between 2015-2017. Climate is temperate continental [24]. The field had clay-loam soil type, with good nitrogen, phosphorus, potassium and micronutrient content, as well as an average humus level. Topsoil had neutral pH [25]. Temperatures and precipitation levels during the experimental period are presented in Figure 1a  In the first year of the experiment, during maize growing season: 28 April-24 September 2015, occurred episodes of low precipitation levels during sowing and sprouting as well as during flowering and grain filling phenophases (July) in addition to high temperatures registered over summer, these caused poor pollination resulting in barren ear tips, low filling ability, and fast loss of kernel moisture. The second experimental year: 27 April-29 September 2016, presented optimal conditions for this crop, temperature and precipitation levels during sowing and sprouting ensured uniformity of seedlings. During the third experimental year: 4 May-25 September 2017, weather conditions were favorable for maize development although the lowest monthly average precipitation levels registered during the experiment occurred in June 2017. During the experiment, summer months registered higher monthly average temperatures compared to the average temperature for the last 58 years. Precipitation levels were below the multiannual average for June 2017 and July 2015 (Figure 1a,b).
The experimental trial consisted of the interaction of three factors: [years (3) × treatments (4) × lines (5)] × two replicates, corresponding to 120 experimental plots under conventional cultivation. The cultivated surface had 1172 m 2 . The biologic material was represented by five inbred lines (L): TC 344, TA 426, TC 385A, TA 447, and TA 452. These have been used as the mother parent for the latest hybrids created at ARDS Turda. Treatments consisted of four detasseling methods (D) applied to plants: D1-control/no detasseling, D2-tassel alone removed, D3-tassel plus 2 leaves removed, D4-all leaves above the ear were removed. Maize density was 50,000 plants/ha. Plants were openly pollinated.
For each variant, seed yield was determined at harvest on 7 m 2 × 2 replicates, while for the other characters, were determined on 10 ears each × 2 replicates in three consecutive years (Y): 2015-2017.
Statistical tests applied were analysis of variance (ANOVA) and the Fisher least significance difference test (LSD). Least significance values at p levels 0.05, 0.01, and 0.001 for each character studied are provided in Table S1 from the Supplementary file, in addition, the standard deviation and standard error for key agronomic traits are found in Table S4.

Seed Yield
Experimental factors, as well as their interaction, exercised significant influence on seed yield (Table S2, Supplementary file). But the strongest influence was exercised by experimental years followed by detasseling methods which together explain 60% of the overall variance. The inbred line alone explains only 12.7% of the overall variance for seed yield. Interactions between factors explain 24.9% of overall variance ( Figure 2). Agronomy 2020, 10, x FOR PEER REVIEW 3 of 10 In the first year of the experiment, during maize growing season: 28 April-24 September 2015, occurred episodes of low precipitation levels during sowing and sprouting as well as during flowering and grain filling phenophases (July) in addition to high temperatures registered over summer, these caused poor pollination resulting in barren ear tips, low filling ability, and fast loss of kernel moisture. The second experimental year: 27 April-29 September 2016, presented optimal conditions for this crop, temperature and precipitation levels during sowing and sprouting ensured uniformity of seedlings. During the third experimental year: 4 May-25 September 2017, weather conditions were favorable for maize development although the lowest monthly average precipitation levels registered during the experiment occurred in June 2017. During the experiment, summer months registered higher monthly average temperatures compared to the average temperature for the last 58 years. Precipitation levels were below the multiannual average for June 2017 and July 2015 (Figure 1a,b).
The experimental trial consisted of the interaction of three factors: [years (3) × treatments (4) × lines (5)] × two replicates, corresponding to 120 experimental plots under conventional cultivation. The cultivated surface had 1172 m 2 . The biologic material was represented by five inbred lines (L): TC 344, TA 426, TC 385A, TA 447, and TA 452. These have been used as the mother parent for the latest hybrids created at ARDS Turda. Treatments consisted of four detasseling methods (D) applied to plants: D1-control/no detasseling, D2-tassel alone removed, D3-tassel plus 2 leaves removed, D4all leaves above the ear were removed. Maize density was 50,000 plants/ha. Plants were openly pollinated.
For each variant, seed yield was determined at harvest on 7 m 2 × 2 replicates, while for the other characters, were determined on 10 ears each × 2 replicates in three consecutive years (Y): 2015-2017.
Statistical tests applied were analysis of variance (ANOVA) and the Fisher least significance difference test (LSD). Least significance values at p levels 0.05, 0.01, and 0.001 for each character studied are provided in Table S1 from the Supplementary file, in addition, the standard deviation and standard error for key agronomic traits are found in Table S4.

Seed Yield
Experimental factors, as well as their interaction, exercised significant influence on seed yield (Table S2, Supplementary file). But the strongest influence was exercised by experimental years followed by detasseling methods which together explain 60% of the overall variance. The inbred line alone explains only 12.7% of the overall variance for seed yield. Interactions between factors explain 24.9% of overall variance ( Figure 2).  All inbred lines registered a decrease of seed yield in response to the application of the three detasseling methods (Figure 3). The yield reduction for the five inbred lines averaged 4% for inbred line TA 426 and 21% for inbred line TC 344 when only the tassels were removed. When the tassel was removed together with two leaves, seed yield decreased by 22% for the line TA 452 and 31% for the line TC 344. In the case of removing all the leaves above the main ear, seed yield losses were highly significant compared to the control. Both lines TC 344 and TA 426 recorded a seed yield loss of 48% while the highest loss of seed yield was registered for TC 385A (66%). The lowest seed yield decrease occurred for the lines TA 447 of only 31% followed by TA 452 with a decrease of 34%. These two last lines have on average 4.4 and 4.5 leaves per plant above the main ear, respectively compared to the other three lines which present between 6.0-6.5 leaves per plant above the main ear. Thus, it was interesting to observe the lines that present a higher number of leaves above the main ear present also the highest seed yield losses following the detasseling method which produces major damage to plants by removing all leaves above the main ear (D4). At the same time, a line characterized by a lower number of leaves above the main ear (TA 447) recorded the lowest seed yield loss following removal of all leaves above the main ear. For the lines TC 344 and TC 385A, the seed yield losses were significant both in the case of the removal of tassels with two leaves (21%; 27%) and especially after removal of all leaves above the main year (48%; 66%). Probably leaves left on the plant in the case of lines with a lower number of leaves per plant above the main ear, have a higher photosynthetic capacity. Yield responses to leaf removal varied between inbred lines. When tassels alone were removed (D2), a decrease of the seed yield was insignificant for two inbred lines: TA 426 and TA 452. For the other three inbred lines, the decrease in yield was significant, especially for TC 344 and TC 385A (Figure 3).
Agronomy 2020, 10, x FOR PEER REVIEW 4 of 10 All inbred lines registered a decrease of seed yield in response to the application of the three detasseling methods (Figure 3). The yield reduction for the five inbred lines averaged 4% for inbred line TA 426 and 21% for inbred line TC 344 when only the tassels were removed. When the tassel was removed together with two leaves, seed yield decreased by 22% for the line TA 452 and 31% for the line TC 344. In the case of removing all the leaves above the main ear, seed yield losses were highly significant compared to the control. Both lines TC 344 and TA 426 recorded a seed yield loss of 48% while the highest loss of seed yield was registered for TC 385A (66%). The lowest seed yield decrease occurred for the lines TA 447 of only 31% followed by TA 452 with a decrease of 34%. These two last lines have on average 4.4 and 4.5 leaves per plant above the main ear, respectively compared to the other three lines which present between 6.0-6.5 leaves per plant above the main ear. Thus, it was interesting to observe the lines that present a higher number of leaves above the main ear present also the highest seed yield losses following the detasseling method which produces major damage to plants by removing all leaves above the main ear (D4). At the same time, a line characterized by a lower number of leaves above the main ear (TA 447) recorded the lowest seed yield loss following removal of all leaves above the main ear. For the lines TC 344 and TC 385A, the seed yield losses were significant both in the case of the removal of tassels with two leaves (21%; 27%) and especially after removal of all leaves above the main year (48%; 66%). Probably leaves left on the plant in the case of lines with a lower number of leaves per plant above the main ear, have a higher photosynthetic capacity. Yield responses to leaf removal varied between inbred lines. When tassels alone were removed (D2), a decrease of the seed yield was insignificant for two inbred lines: TA 426 and TA 452. For the other three inbred lines, the decrease in yield was significant, especially for TC 344 and TC 385A ( Figure 3).

Ear, Cob, and Kernel Traits
Maize seed yield decrease was the result of a decrease of ear weight and length, kernels weight/ear, the number of kernels/row and number of rows/ear. All these traits were significantly influenced by detasseling (D), genotype (L), and their interaction (D × L). Regarding the differences between inbred lines for analyzed characters, the highest ear weight and kernel weight were recorded by TA 426 and TA 447, the longest ear and the highest number of kernels/row for line TC 385A, and highest row number/ear for line TA 426 (Figures 4 and 5).
However, genotype had a higher influence on the ear characters except for the ear weight and kernels weight/ear, than detasseling. Additionally, triple interaction of the factors has a higher influence on the ear weight than on the ear length. A decrease in the number of kernels per row and ear length significantly influenced the yield differences between variants (Table S3, Supplementary file).

Ear, Cob, and Kernel Traits
Maize seed yield decrease was the result of a decrease of ear weight and length, kernels weight/ear, the number of kernels/row and number of rows/ear. All these traits were significantly influenced by detasseling (D), genotype (L), and their interaction (D × L). Regarding the differences between inbred lines for analyzed characters, the highest ear weight and kernel weight were recorded by TA 426 and TA 447, the longest ear and the highest number of kernels/row for line TC 385A, and highest row number/ear for line TA 426 (Figures 4 and 5).
However, genotype had a higher influence on the ear characters except for the ear weight and kernels weight/ear, than detasseling. Additionally, triple interaction of the factors has a higher influence on the ear weight than on the ear length. A decrease in the number of kernels per row and ear length significantly influenced the yield differences between variants (Table S3, Supplementary file).
Ear traits varied highly between inbred lines in response to the removal of leaves. Removing all the leaves above the main ear (D4) had a negative effect on most of the ear's characters: ear weight Agronomy 2020, 10, 729 5 of 10 and length, kernels weight/ear, and the number of kernels per row for the five lines (Figures 4 and 5). Removal of the tassel with two leaves resulted in a decrease of ear weight with 10-12% relative to control and among the five genotypes analyzed, the inbred line TC 385A proved to be more sensitive (Figure 4).
Agronomy 2020, 10, x FOR PEER REVIEW 5 of 10 Ear traits varied highly between inbred lines in response to the removal of leaves. Removing all the leaves above the main ear (D4) had a negative effect on most of the ear's characters: ear weight and length, kernels weight/ear, and the number of kernels per row for the five lines (Figures 4 and  5). Removal of the tassel with two leaves resulted in a decrease of ear weight with 10-12% relative to control and among the five genotypes analyzed, the inbred line TC 385A proved to be more sensitive (Figure 4).  The kernels weight/ear decreased with 10% for TA 452, with 21% for TA 447 and with 52% for TC 385A (Figure 4). In the case of the other two detasseling methods, inbred lines behaved differently. All three detasseling methods (D2, D3, D4) resulted in a highly significant decrease in the number of Ear traits varied highly between inbred lines in response to the removal of leaves. Removing all the leaves above the main ear (D4) had a negative effect on most of the ear's characters: ear weight and length, kernels weight/ear, and the number of kernels per row for the five lines (Figures 4 and  5). Removal of the tassel with two leaves resulted in a decrease of ear weight with 10-12% relative to control and among the five genotypes analyzed, the inbred line TC 385A proved to be more sensitive (Figure 4).  The kernels weight/ear decreased with 10% for TA 452, with 21% for TA 447 and with 52% for TC 385A (Figure 4). In the case of the other two detasseling methods, inbred lines behaved differently. All three detasseling methods (D2, D3, D4) resulted in a highly significant decrease in the number of The kernels weight/ear decreased with 10% for TA 452, with 21% for TA 447 and with 52% for TC 385A (Figure 4). In the case of the other two detasseling methods, inbred lines behaved differently. All three detasseling methods (D2, D3, D4) resulted in a highly significant decrease in the number of rows/ear for line TC 344. Lines TA 447 and TA 452 presented no statistically significant response to either detasseling method for the number of rows/ear ( Figure 5).
Inbred lines and detasseling methods significantly influenced all analyzed kernel traits (Table S3, Supplementary file). Detasseling and the interaction between detasseling and inbred line had a stronger influence on TKW (thousand-kernels weight) than on kernels dry matter and on kernel Agronomy 2020, 10, 729 6 of 10 depth. Triple interaction Y × D × L had significant influence only on kernel depth and cob diameter. The experimental years did not influence significantly TKW (thousand-kernels weight) but exercised statistically significant influence on the other kernel traits (Table S3, Supplementary file). Out of the five inbred lines tested, only TA 452 experienced a significant decrease of kernel dry matter following D2 and D3 detasseling methods. The application of the D4 detasseling method caused a highly significant decrease of TKW for the line TC 385A (Figure 6). rows/ear for line TC 344. Lines TA 447 and TA 452 presented no statistically significant response to either detasseling method for the number of rows/ear ( Figure 5).
Inbred lines and detasseling methods significantly influenced all analyzed kernel traits (Table  S3, Supplementary file). Detasseling and the interaction between detasseling and inbred line had a stronger influence on TKW (thousand-kernels weight) than on kernels dry matter and on kernel depth. Triple interaction Y × D × L had significant influence only on kernel depth and cob diameter. The experimental years did not influence significantly TKW (thousand-kernels weight) but exercised statistically significant influence on the other kernel traits (Table S3, Supplementary file). Out of the five inbred lines tested, only TA 452 experienced a significant decrease of kernel dry matter following D2 and D3 detasseling methods. The application of the D4 detasseling method caused a highly significant decrease of TKW for the line TC 385A (Figure 6). A significant decrease of ear diameter following detasseling was recorded by four out of five inbred lines and had no significant effect on one of them: TA 452. Detasseling exercised a significantly negative influence on cob diameter and kernel length for two inbred lines (Figure 7). Inbred lines and detasseling methods significantly influenced all analyzed kernel traits (Table  S3, Supplementary file). Detasseling and the interaction between detasseling and inbred line had a stronger influence on TKW (thousand-kernels weight) than on kernels dry matter and on kernel depth. Triple interaction Y × D × L had significant influence only on kernel depth and cob diameter. The experimental years did not influence significantly TKW (thousand-kernels weight) but exercised statistically significant influence on the other kernel traits (Table S3, Supplementary file). Out of the five inbred lines tested, only TA 452 experienced a significant decrease of kernel dry matter following D2 and D3 detasseling methods. The application of the D4 detasseling method caused a highly significant decrease of TKW for the line TC 385A (Figure 6).

Discussion
Maize yield and grain quality are the results of the interaction between genetic, environmental, and agronomic factors [11,[26][27][28]. Overall, results are in accordance with previous studies which suggest that plant damage can have more or less serious consequences on seed yield, depending on both genotype and environmental conditions [23]. Not all genotypes are well suited for mechanical detasseling while most suitable ones are those that have a high degree of uniformity. Often, mechanical detasseling must be supplemented by hand detasseling to remove missed or late-maturing tassels.
The results of this study demonstrate that detasseling can cause seed yield losses in all inbred lines tested. By removing the tassels alone yield losses were between 4-21%. Removal of all leaves above the main ear caused highly significant loss of seed yield, with a decrease between 31-66%, while two out of five inbred lines (TA 447 and TA 452) produced about 4000 kg/ha even after plants were cut above the main ear. Results suggest that seed quantity and quality can decrease significantly as a result of the reduction of the plant photosynthetic area. Because grain filling occurs mainly from photo-assimilates produced post-anthesis, it is understandable why the reduction of leaf area, particularly the removal of leaves above the main ear, which are considered the most photosynthetically active, can have negative consequences for maize grain yield and other grain traits [29] as reported in previous studies [29][30][31][32].
One study shows that for Pioneer 30F90 simple maize hybrid in conditions of Paraná, Brazil, removal of tassel together with 4-5 leaves caused a significant decrease in the number of grains per row [29]. Another study conducted in southern Brazil, in conditions from Minas Gerais, reports that maize hybrid NB7376 registered 20% seed yield decrease and 8% TKW decrease following removal of all leaves above the main ear [30]. A study that compared the effects of five types of detasseling methods on two lines and one hybrid (CMS 355) concluded that manual detasseling or the use of male-sterile genotypes are better practices since whorl pull-off, as well as mechanical detasseling, negatively affects the seed production of maize plants [31]. Machine detasseling caused a 24.5% seed yield decrease in AG 122 hybrid Monsanto when the machine was programmed to remove 90% of the tassels with tires [32].
Because genotype exhibits different levels of phenotypic expression under the influence of environmental conditions, a large genotype by environment interaction can occur under stressful conditions [33]. This further explains why detasseling damage acting as abiotic stress for the plant causes high seed losses particularly in years less favorable for the development of this plant and why there is a variation of this seed decrease among inbred lines. Because some lines seem less affected compared to others following extensive plant damage, clearly there are genotypes more suitable for mechanical detasseling. Pin-pointing the underlying physiologic and molecular mechanisms and identifying an array of potential markers representative of the plant's ability to cope with the stress induced by damage caused to the plants could prove useful in screening existing maize germplasm collections for this type of tolerance. This becomes even more pressing due to the transition to full mechanization in agriculture and where identification of suitable inbred lines more tolerant to damage induced by mechanized detasseling can ultimately assist in the optimization of seed production. Similarly, other authors remark the lack of maize germplasm designated as suitable for full crop mechanization prompting the need for more research [34].
Besides genotype, climatic parameters such as water availability and temperature levels are recognized as key factors responsible for maize crop productivity [26,27,29] and rainfall between April-August has a higher influence on maize yield than nitrogen fertilizer [8]. Particularly, maize is most sensitive to drought stress during pollination and grain-filling [35,36]. As noted in this study, under the influence of a continental climate, weather can act as a strong abiotic conditioning factor for maize seed yield. To reduce this effect, the precision of detasseling could be decided depending on environmental conditions in order to minimize plant damage and consequently seed losses in unfavorable years. Cost efficiency can be reached both by applying mechanical detasseling for lines that show lesser seed yield loss or by limiting manual detasseling only to valuable inbred lines that prove sensitive to plant damage caused by incorrect or fast mechanical detasseling. From the perspective of future maize breeding programs, results suggest that given the climatic particularities of Romania, maize genotype stability to environmental factors and adaptation to local conditions should become key performance criteria for maize genotypes. Due to the current climate trend at a global level, there is a particular need for enhancing maize tolerance to drought and heatwaves [37], especially since Agronomy 2020, 10, 729 8 of 10 climatic models predict more frequent and severe extreme weather events with serious impact for cereal crops and implicitly for food security [38].

Conclusions
This research explored the relationship between genotype, climatic conditions, and detasseling method on seed yield of five inbred lines during a three-year simulation experiment, with the purpose to identify ways to optimize the maize seed production technology.
Results indicate that seed yield decreases directly proportional to the extensiveness of damage induced to plants. Maize genotype determines the response to detasseling, with inbred lines that present a higher number of leaves above the main ear as the genotype-trait experiencing the highest seed yield loss following extensive plant damage compared to inbred lines which present a lower number of leaves per plant above the main ear.
Continental climate conditions with fluctuations from one year to another can exercise strong influence over quantitative maize characters.