The Prospect of Bentazone-Tolerant Soybean for Conventional Cultivation

: Soybean is one of the most important crops widely used as food, feed, and industrial products. Weeds compete with the crops for light, nutrients, water, space, and other growth requirements, causing an average yield reduction of 37% in soybean. Bentazone is an herbicide that selectively kills broadleaf weeds by inhibiting photosynthesis. It is widespread in soybean-cultivating areas including genetically modiﬁed organism (GMO) and GMO-free regions all around the world. This herbicide carries a double-edged sword since it can also incur damage to crops upon application. The challenge, therefore, lies in the deliberate selection of bentazone-tolerant cultivars. We systematically analyzed several factors that a ﬀ ect the metabolism of bentazone in soybean, such as phenotypic and genotypic di ﬀ erences among soybean accessions, the time and method of application, the absorption and metabolism of bentazone in soybean, and the e ﬀ ects of e ﬀ ective application and cultivar selection on yield and crop injury. Furthermore, we recommend that further studies should be done in aid of discovering more tolerant soybean cultivars. We hope that our review will help farmers and soybean producers, as well as geneticists, in developing bentazone-tolerant soybean cultivars to improve the global yield of soybean crops as a response to the ever-dynamic food demand across the globe.


Introduction
Weeds reduce farm and forest productivity, invade crops, smother pastures, and harm livestock in several cases [1]. Weeds compete with crops for light, nutrients, water, space, and other growth requirements, thereby reducing the crop quality and yield. They also produce harmful chemicals that affect the growth of associated crops (allelopathic effect) and serve as an alternative host to pests and diseases of crops [2]. In soybeans (Glycine max (L.) Merr.), weeds alone can cause an estimated average reduction of 37% in yield, whereas other fungal diseases and agricultural pests account for 22% of losses [3]. On average, soybean yield in organic systems was 19% lower than that in conventional systems, and weed competition alone accounted for this difference [4]. Yield loss for soybean grown with pigweed (Amaranthus palmeri), crabgrass (Digitaria sanguinalis), and common ragweed (Ambrosia artemisiifolia) ranged from 14% to 37%, 0% to 37%, and 40% to 95%, respectively [5]. Weed management is essential for any current agricultural production system, especially for large monoculture areas that are affected by a great extent of environmental pressure. Soybean is one of the largest monocultures in the world, with 122.44 million ha harvested in 2019 [6].
Management of unwanted weeds and other plants should be cost-effective for agricultural and other related industries. Herbicides can provide cheap weed control and reduce workload, because

Bentazone
Herbicides are selected based on several factors for successful weed control, including weed range, lack of crop injury, effect of agro-ecosystem, economic cost, and policy. Bentazone [3-Isopropyl-1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide] was initially registered in 1975. It has been used for selective POST control of broadleaf weeds and sedges in alfalfa, asparagus, cereals, clover, digitalis, dry peas, flax, garlic, grass, green lime beans, mint, onions, potatoes, snap beans for seeds, sorghum, soybeans, and sugarcane [20][21][22][23]. Bentazone is a contact herbicide that interferes with susceptible plants' ability to use sunlight in producing survival energy through photosynthesis. Visible damages on the surface of leaves were usually observed after a few hours to weeks of treatment with bentazone, depending on the species and dosage [24][25][26]. Bentazone is absorbed by leaves upon application as a foliar spray, accompanied by rapid break-down or metabolism into natural plant components and metabolites in resistant plants, such as starch, protein, lignin, amino acid, and cellulose [26]. When bentazone is absorbed by the roots, it is translocated from the roots to other parts of the plants through xylem, with plant nutrients in the transpiration stream [27]. However, the degree of translocation is dependent on plant species after absorption by leaves. In susceptible and resistant soybean genotype, the amount of absorbed bentazone translocated from the treated leaf was 7 to 13 percent of the total absorption [28]. In addition, foliar application of bentazone to soybean resulted in slow acropetally translocation of the herbicide [29]. In tolerant rice, most of the 14 C-bentazone remained in the treated leaf or translocated to older leaves, whereas most of the 14 C-bentazon was translocated from the treated leaves to younger leaves in susceptible rice [26]. Acropetal and basipetal translocation of absorbed 14 C from 14 C-bentazon was equal in corn [30]. Most of the absorbed 14 C-bentazone in capsicum species remained in the treated leaves, with minimal acropetal and basipetal translocation from the site of application [31]. For some susceptible weed species (yellow nutsedge, Canada thistle) the translocation was acropetal transport [29,32].
The adverse effect of bentazone on agro-biodiversity as well as soil fertility is less than that of other selective contact herbicides [33]. Bentazone concentration at the field does not considerably affect the microflora even without microbial degradation [34,35]. In case of 2,4-D, it adversely affects the activities of Rhizobium sp. and glyphosate suppresses the phosphatase activity of purple non-sulfur bacteria in soil, decreasing the growth and activity of azotobacter as well as N-fixation in soybean [36]. Compared with other POST broadleaf herbicides, bentazone is quickly degraded in the soil [37,38]. For soil fertility, bensulfuron methyl and metsulfuron-methyl reduces the operation of N-mineralization. In addition, prometryn, simazine, and terbutryn inhibit not only the N-fixation, but also reduce the total number of nodules and N in soil. Furthermore, glyphosate, sulfonylurea and atrazine also dramatically alter soil structure with the disturbances in soil earthworm ecology and the inhibition of soil N-cycle under alkaline or low organic matter [39].
Since bentazone was originally evaluated by the Joint FAO/WHO meeting on Pesticide Residues (JMPR) in 1991, the re-evaluation was periodically conducted until now. The evaluation reported residue of parent bentazone and its metabolites such as 6-and 8-hydroxy-bentazone atter its application in seed, food, and feed of plant. In USA, the limit of detection by gas liquid chromatography method for compounds of bentazone and its metabolites in soybean was 0.05 ppm according to a rule by the environmental protection agency in 2019 [40]. In Europe, the maximum residue level for soybean was 0.01 ppm based on a report of pesticide residues in food in 2013 [41]. In addition, based on carcinogen assessment by environmental protection agency, bentazone was found not to be mutagenic and was classified as a Group E chemical, which means evidence of non-carcinogenicity for humans [42].

Bentazone Effectivity Modulated by Environmental Conditions
The effective utilization of herbicides is determined by environmental conditions before, during, and after its application [43]. Increasing light intensity at the application of herbicide results in higher photosynthesis and subsequent phloem translocation to increase the movement of foliar-applied herbicides. The stomata remain open at high light intensity, thus increasing tissue penetration of foliar herbicides [43]. Thus, the effectiveness of POST herbicides depends on light intensity during application.
One of the most important environmental factors are air temperature and relative humidity (RH), which alter the performance of herbicides in weed control. Absorption and translocation of bentazone in plant increases with higher temperatures [44]. At high temperatures, herbicide absorption is enhanced through reduced cuticle waxes and increased herbicide diffusion through the cuticle [43]. With high temperatures and high RH levels, the cuticle is highly hydrated, thereby promoting absorption of herbicides [45].
Generally, humidity has a greater effect on herbicide uptake than temperature [46]. Increased humidity increases the uptake of bentazone. For instance, increased uptake of bentazone at 80% RH rather than one at 40% RH was observed in seven plant species [44]. Additionally, high RH levels favor the efficiency of foliar-applied herbicides by affecting herbicide uptake through interactions between the herbicide droplet, leaf cuticle, stomatal opening, and water in or around droplets [47]. The effects of low humidity on bentazone uptake can be overcome by adding adjuvants. For example, enhancement of bentazone uptake by adjuvants was more pronounced at 40% RH than at 80% RH [44]. Bentazone was found to be very mobile in soil at natural RH due to its high-water solubility [47]. Bentazone's herbicidal activity is very low in dry soil; however, its activity increases with increasing soil moisture content and highly active at slightly flooded conditions [47]. Herbicide performance is generally reduced on moisture stressed plants under dry or low soil moisture conditions because plant morphology and physiology are both affected [48]. Understanding of the environmental factors that cause low herbicide efficiency makes it possible to consider the appropriate conditions such as temperature and humidity to spray, thereby maximizing the dose applied and minimizing the processing costs.

Tolerance Mechanism for Bentazone
The WSSA defines herbicide resistance as "the inherited ability of a plant to survive and reproduce following exposure to a dose of herbicide normally lethal to the wild type, and may be naturally occurring or induced by techniques, such as genetic engineering or selection of variants produced by tissue culture or mutagenesis" [49]. Herbicide resistance mechanisms include structural reforms at the target site of the herbicide, metabolic detoxification, and changes in herbicide uptake, translocation, or compartmentalization [50]. Plant species that are not controlled by a herbicide before any selection pressure or genetic manipulation can be considered naturally tolerant, but not herbicide-resistant [51].
Bentazone tolerance requires mechanisms that limit the amount of active herbicide entering the target site, mechanisms that decrease herbicide uptake, and mechanisms that improve herbicide metabolism. Differential absorption and metabolism in treated bentazone-susceptible line leaflets resulted in a higher bentazone concentration than that of tolerant species, revealing that differential metabolism is the key cause of different responses to bentazone [30,52]. Tolerant plants can easily detoxify bentazone through rapid aryl hydroxylation followed by glucose conjugation, whereas susceptible plants metabolize bentazone to a lesser extent, and several species are unable to metabolize it at all. The absorbed bentazone was hydroxylated to either 6-hydroxy-bentazone or 8-hydroxy-bentazone and then glycosylated at positions 6 or 8 of its aromatic ring, which was eventually oxidized to produce natural plant products, such as starch, protein, lignin, amino acid, and cellulose [53]. Base on the species, the positions of its aromatic ring of hydroxylation and glycosylation are different. Bentazone is readily metabolized in rice and other gramineous plants by detoxifying this herbicide primarily by 6-hydroxylation or 6-and 8-hydroxylation and glucose conjugation of its hydroxylated positions [54]. Sorghum and Johnsongrass [55] rapidly metabolizes bentazone by producing 6-hydroxylbentazone, whereas wheat [54], corn [56], and soybean [28] metabolizes it by producing 6-and 8-hydroxyl-bentazone after hydroxylation followed by glycosylation at the 6 and 8 positions of its aromatic ring.
Metabolic resistance of bentazone are generated by endogenous cytochrome P450 mono-oxygenases, glucosyl transferases (GTs), glutathione S-transferases (GSTs), and/or other enzyme systems, such as aryl acylamide [57]. Hydroxylation of aromatic rings or alkyl groups by a family of enzymes known as cytochrome P450 mono-oxygenases and then, glucose conjugation at positions of its aromatic ring by GSTs or GTs is the most common way to detoxify herbicides in plants [58,59].
In previous studies, the cytochrome P450 family has been identified to detoxify bentazone in various plants [23,56,70]. In rice, a Bel-encoded cytochrome P450 mono-oxygenase, CYP81A6, mediated resistance to bentazone. The loss of bentazone resistance in rice resulted from a single base-pair deletion of CYP81A6 [23,71]. Additionally, Fang et al. [72] reported that a 519-kD transcription factor-like protein (B9FDA1) containing two histidine kinase-like ATPase domains and a zinc-finger type C3HC4 RING domain can serve as a transcription factor for the expression of bentazone tolerance genes in rice.
In soybean, the gene expression pattern of certain cytochrome P450 homologs, such as CYP94A1, CYP82A2, CYP71D8, and CYP81E8, occurred when plants were treated with bentazone [28]. Bernard and Wax [73] reported that the inheritance of bentazone sensitivity in soybean was controlled by a single recessive gene. A single recessive gene (bnz-1) was mapped to chromosome 16 for bentazone sensitivity of soybean, where a single base-pair deletion in the coding region of GYP81E22 (Glyma.16G149300) at +1465 bp downstream from the translation start codon led to a premature stop codon and loss of function of cytochrome P450 hydroxylase [74]. Several compounds inhibit both soybean aryl hydroxylation and cytochrome P450 reactions, provided by in vivo evidence of bentazone metabolism facilitated by cytochrome P450 [70]. For GTs, Leah et al. [70] isolated and purified two enzymes from bentazone-tolerant soybean to produce the glycosylated 6-or 8-hydroxybentazone.
POST application of bentazone reported initially the susceptibility of various in-breeds in the study of inheritance of single crosses of corn, which may be attributed to a single recessive gene, named ben [61]. Several studies supported that the ben locus may be responsible for bentazone sensitivity by cytochrome P450 monooxygenase in white-kernel inbred [75] and sweet corn [76]. In addition, another study of an in vitro assay with corn reported that bentazone aryl hydroxylation of maize microsomes is catalyzed by cytochrome P450 mono-oxygenase [56].
Bentazone tolerance of Capsicum annuum was also controlled by a single dominant gene [77]. A dominant Bzt gene responsible for bentazone tolerance in pepper cultivar "Santaka" may be the same gene or may be closely linked to the Bzt locus that confirms bentazone tolerance in "Bohemian Chili" [66].
New technologies involved in genetically modifying herbicide-resistant cultivars help in managing alternatives to glyphosate. Several of them that are still being tested may be available for release on short notice [78]. Bentazone-resistant species of cotton [79,80], tobacco, and Arabidopsis [81] were developed through genetic transformation of the bentazon-resistant gene (Cyp81A6) from rice. Additionally, rat CYP1A1 was transformed for potato bentazone-resistant cultivars [82].

Pre-and Post-Emergence Herbicides for Soybean Production
Soybean is one of the most important crops and is widely used as a staple for human food, animal feed, and industrial products globally. Soybeans are high in protein (40%), and they are a major ingredient in livestock feed. Soybeans are processed for their oil and meal, and a smaller percentage is processed for human consumption and made into soy milk, soy flour, soy protein, tofu, and many other retail food products [83].
Despite having advanced technologies, weeds are considered the number one problem in major soybean-producing countries. Weed management strategies depend on significant biological factors, such as weed and crop intensity, seedbank processes, demographic variability, and weed-crop rivalry and reproductive biology [84]. Before starting weed management in soybean fields, we should think about resources, and how and when to use them. Apparently, due to countless cases of resistance, weed control strategies that integrate traditional, physical, and chemical controls are preferred [85]. Herbicides are important tools in weed control in the wide spectrum of practice, particularly for those with a large number of chemical-resistant crops.
During initial establishment, PRE herbicides can help farmers maintain weed-free fields and also reduce pressure on POST herbicides. PRE herbicides can be applied before or up to 3 d after planting. Tillage and PRE herbicides were the most popular weed control systems in soybeans in the 1970s [85]. Dinitroanilines, fluchloralin, and vernolate were used in pre-planting and incorporated for annual grass and broadleaf weed control [85]; triazines were also used in PRE applications for small seeded broadleaves, often in combination with alachlor to improve annual grass control [86]; and linuron, offering broad spectrum control, was also used in PRE application [87]. There have been many weed issues, such as perennial grasses and the usual widespread weeds of summer crops, due to the limited choices in soybean herbicides. The creation of several new chemicals during the 80s and 90s improved weed control, although their application is still combined with mechanical tools, before glyphosate-resistant soybeans were introduced [85]. Acifluorfen, a chemical that controls large-seeded broadleaf weeds, has become a common treatment rescue method, although it has caused serious crop injury [88]. It was partly replaced by another diphenylether, fomesafen; however, it still lacked the same efficacy or control spectrum [89]. The registration of acetolactate synthase-inhibiting herbicides (sulfonylureas, imidazolinones, and triazolopyrimidines) has sparked a new era of soybean weed control, allowing for PRE/POST combinations and providing effective and persistent control of the most important weeds, with less crop injury than previous chemicals [90]. Several recently developed PRE herbicides with advanced options, such as flexibility across tillage systems, multiple modes of action, longer residual effects, and higher yield potential, are BroadAXE, prefix, boundary [91].
Glyphosate is a non-selective herbicide, and it can be applied from emergence to full flower season in glyphosate-resistant soybean field.
Carfentrazone, sulfentrazone (aryl triazinones), and flumioxazine (an N-phenylphtalimide derivative) offered new options for burndown (carfentrazone) and residual control (sulfentrazone and flumioxazine); however, the introduction of the glyphosate-resistant soybean varieties prevented their utilization, with the launch of glyphosate-resistant varieties in 1996 being the most drastic expansion of soybean output [85].
Soybeans are most sensitive to weed competition at 4-7 weeks after emergence [93]. The window to control broadleaf weeds is very small, and the best results are obtained on weeds with less than four leaves. POST broadleaf weeds may be controlled with Spinnaker ® (Imazethapyr), Broadstrike ® (flumetsulam), Basagran ® (bentazone), Blazer ® (Acifluorfen), and Raptor ® (imazamox). Mixtures of Basagran ® and Blazer ® have been commonly used to increase the activity spectrum. The most current herbicides used for weeding management in corn, soybean, and cotton are selective and typically used in mixtures to control a broad spectrum of weed species [90].
Although glyphosate provided the most effective weed control when applied with other broadleaf herbicides [94], researchers at the University of Wisconsin compiled results from a variety of trials conducted in eight northern states, finding that Roundup Ready soybean varieties yields were 4% lower than conventional soybean varieties yields, on average, in 1998 [95]. Thus, farmers need to act now to diversify their herbicides, methods, crops, and their traditional practices.

Utilization of Bentazone in Soybean Production
Bentazone is a POST broadleaf herbicide that is widely used in soybeans. Annual broadleaf weed species can reduce soybean yield by more than 50%. Ambrosia spp. and Amaranthus spp. are frequently reported in literature and regarded as the most noxious broadleaf weeds in soybean. Bentazone prevents weed growth in grain-type soybean as well as vegetable soybean fields [96]. Soybean yield and harvestable soybean losses were prevented by pre-harvest application of a mixture of bentazone and acifluorfen, as well as bentazone and imazamox. The total weed control output of bentazone in conjunction with imazamox and typhenesulfuron was 95.04% [97]. Application of glyphosate tank mixtures with bentazone was more selective to Roundup Ready soybean when applied at younger stages [98].

Genetic Resources and Genetic Information for Bentazone Tolerance in Soybean
There are a number of reports of differential responses to herbicides by crop cultivars. Differential cultivar response is displayed in a number of ways, including different injury symptoms, growth retardation, and possible effects on yield [110]. Identifying and characterizing these differences may be helpful in preventing substantial crop injury and developing more tolerant lines for the future. A total of 338 soybean germplasms in the collection of the US Department of Agriculture were evaluated under field conditions. "Beeson", "Amsoy", "Amsoy 71", "Calland", "Clark 63", and "Corsoy" were found to be bentazone-tolerant, whereas one US cultivar, "Hurrelbrink", and ten Japanese lines were highly sensitive to bentazone [111].
Other studies reported that different absorption and metabolism rates between bentazone-tolerant and bentazone-sensitive soybean resulted in a greater concentration of bentazone in the treated leaflet of susceptible cultivars than in that of tolerant cultivars after foliar treatment. Different bentazone metabolism rates appear to be the main cause of the different responses of these soybean cultivars to bentazone [52]. An experiment was performed to reconfirm the tolerant cultivars' differential metabolism of bentazone, which indicated that tolerant genotypes are 100-300 times more tolerant to bentazone than susceptible genotypes. Tolerant genotypes ("Hill" and "Clark 63") metabolized 80-90% of ingested bentazone within 24 h, whereas sensitive genotypes ("L78-3263", "Hurrelbrink", and "PI229342") metabolized just 10-15% [28]. A vegetable-type soybean, AVS-4002, which is resistant to bentazone as a POST application of tank mixture of bentazone and imazamox on vegetable soybean, was found to have no harmful effects, such as crop injury, and did not affect yield [112].
Recently, we screened 410 cultivated soybeans, 384 wild soybeans (Glycine soja Sieb. and Zucc.), and 1300 mutant lines derived from 0.3% ethyl methanesulfonate (EMS) to select either bentazone-tolerant or -sensitive lines. The reaction of soybean lines to bentazone was normally distributed and several tolerance sensitive lines were found. Among them ten sensitive lines were compared in terms of sequence variation for Glyma.16G149300, which controls bentazone sensitivity in soybean. Our results showed that there was no deletion at the physical position 30,994,760 among 10 soybean lines. However, we found a haplotype, including four single nucleotide polymorphisms (SNPs) in exon 1 and 2 of Glyma.16G149300 through a sequence analysis of the coding regions of 10 sensitive lines; C-T at the physical position 30,993,331, G-C at 30,993,682, A-G at 30,994,898, and A-C at 30,995,057 ( Figure 1). Since four SNPs were silent mutations, further researches were required to show the expression level of Glyma.16G149300 with 10 sensitive lines. Through sequence analysis, we assume that there may be different gene(s) controlling bentazone sensitivity in these soybean lines. In addition, these selected soybean lines can be used as genetic resources for further genetic studies of bentazone treatment.  [113], PEs were from ethyl methanesulfonate (EMS) mutagenesis of "Pungsannamul", "Suwon98" and "PI97150" are germplasms. YWS689 and YWS566 are wild soybean germplasms. Kato et al. [76] identified that a single-base deletion was observed in the coding region of Glyma.

Future Perspective and Conclusion
Since genetically modified (GM) soybean was developed, growers have switched to adopt the new GM cultivars because of its simplicity and flexibility of weed control that depends on only one herbicide to control a broad spectrum of weeds without crop injury. Globally, GM soybeans accounted for about 82% of the total soybean production area [114]. However, many countries are not deliberately growing genetically modified soybean, because of concern over its adverse impact on human health and environmental problems. The demand for non-GM soybean is relatively high in the European Union (EU), compared with other parts of the world. Of the number of soybean and soybean-derived products consumed by the EU, 95% are imported. The main use of soybean products in the EU is for animal feed [115]. Base on the Food and Agriculture Organization (FAO), bentazone was registered to use as herbicide for soybean seed and forage in France, Germany, Greece, Italy, Spain, the Netherlands, and the USA. In non-GMO (genetically modified organism) areas in EU and Asian countries, application of graminicides and bentazone can control simultaneously both grass and broadleaf weed in bentazone-resistant soybean field, which save time, labor, and application cost. Thus, it is important to develop bentazone-resistant soybeans for non-GM soybean production area.
Soybean resistance or tolerance to POST herbicide is essential. Soybean is a broadleaf plant, and weed control was mainly carried out by the PRE + POST herbicide application system before herbicide-resistant soybeans were produced mainly through genetic modification. However, there is a difference in the responses to bentazone among soybean genotypes; hence, it is necessary to select tolerant soybeans for breeding and for genetic studies. It is also necessary to understand inheritance of resistance to bentazone through population genetic studies such as a genome-wide association study. Several studies have reported on the genes that control bentazone tolerance in several crops; however, few genetic studies have been conducted in the past to clarify the molecular mechanisms of bentazone tolerance in soybean. This is most likely due to the advent of genetically modified herbicide tolerance as a significant selectivity method, as well as the production of strong target-site-specific mutant crops for specific herbicides.
Screening of soybean germplasm collections and mutation breeding to generate new genetic resources are to identify bentazone-resistant and bentazone-sensitive soybean accession. With genetic resources, advances in next-generation technology enable high-density genetic mapping and next-generation sequences (NGS) to identify QTL regions or candidate genes controlling the sensitivity or tolerance of soybean to bentazone. Through a QTL mapping study and NGS analyses, tightly flanked markers or gene-based markers are developed to use the marker-assisted selection for response to bentazone in a breeding program. Introgression of the QTLs or candidate genes responsible for xenobiotic detoxification of bentazone in soybean through conventional and molecular breeding approaches certainly warrants future investigations. Developing bentazone-tolerant soybean varieties can be fairly straightforward, as the source of tolerance is either an elite breeding line or a cultivar. Traditional and modern breeding methods will suffice to transfer resistance to elite breeding materials. When this trait is integrated into the high-yielding background, it will be possible to trade herbicide-tolerant varieties to growers that maintain their yield under herbicide stress conditions. Further increases in tolerance by an additive effect to bentazone may be possible by pyramiding genes controlling hydroxylation and glycosylation of bentazone from several tolerant genotypes in a natural population. In addition, using mutant lines as genetic resources will allow a future increase in bentazone tolerance, since tolerance in each mutant is regulated by a single gene, and the effects of these genes have been cumulative [116]. Since genotypic differences have already led to the development of metribuzin tolerance in lupin, soybean, and wheat [117,118]. stacking genes that control tolerance to different herbicides may be another option to combat resistant weeds. Recently, advances in CRISPR/Cas Genome editing via knockout, knock-in, replacement, and point mutation have enabled efficient modifications of a targeted gene controlling the response to bentazone in soybean, accelerating the development of new cultivars. In conclusion, it is necessary to conduct more research on finding new genes that control tolerance to better understand the tolerance mechanism and to assist in developing tolerant soybean varieties. Even though bentazone controls many broadleaf weeds, it is necessary to test the efficacy of bentazone in controlling various broadleaf weeds in various regions. Bentazone-tolerant soybean cultivars will greatly contribute to weed control in the field for conventional soybean production.

Conflicts of Interest:
The authors declare no conflict of interest.