E ﬀ ect of Some Citrus Essential Oils on Post-Harvest Shelf Life and Physicochemical Quality of Strawberries during Cold Storage

: Utilization of essential oils alone or incorporation with edible ﬁlms is an appropriate technique to conserve the quality attributes and reduce post-harvest deterioration in fresh vegetables and fruits. Strawberries, being perishable fruits have a short shelf life, and using essential oils is considered one of the most suitable methods to prolong their shelf life during storage. The current study assessed the impact of di ﬀ erent essential oils, including lemon oil (L), orange oil (O) and mandarin oil (M) on the physicochemical and microbial load of strawberries ( Fragaria × ananassa cv. Festival) stored at 2 ± 1 ◦ C and 95% relative humidity (RH) for 18 days. The di ﬀ erences in the physicochemical and microbial properties of strawberries were assessed by determining the following parameter changes: weight loss, decay percentage, ﬁrmness, soluble solids content, titratable acidity, color, anthocyanins, vitamin C, total phenol, total antioxidant, catalase activity, polyphenol oxidase activity, sensory evaluation, microbial content, total coliforms, molds, and yeasts. The results of this study indicated that the fruits treated with all essential oils treatments (L, O and M) had higher total antioxidant content and physicochemical properties than untreated fruits, due to protection against the microbial growth of molds, and yeasts. At the end of the storage period, the treated fruits showed a greater acceptance and sensory attributes than the untreated fruits. Furthermore, the correlation study showed a signiﬁcant and negative relationship between the total antioxidant of treated fruits and following quality attributes including, weight loss, decay percentage, respiration rate soluble solids content, polyphenol oxidase activity, molds, and yeasts. It is noteworthy that all the essential oil treatments extended the shelf-life of strawberries and delayed their deterioration up to 18 days.


Introduction
Strawberries (Fragaria × ananassa) are in high demand because of their richness in minerals, vitamins, anthocyanins, flavonoids, and phenolic compounds that play a vital role in the human diet and health [1]. Usually, the fruits of strawberries are consumed fresh or processed in juices. Strawberries are also considered a perishable fruit and extremely sensitive to microbial contamination that can affect their quality traits and diminish nutritional value. Furthermore, the possible existence of pathogens (such as Escherichia coli) and their toxins can even pose a threat to consumer safety and contribute to foodborne illness. Although synthetic fungicides application, mainly benzimidazole, dimethyl compounds possesses great structural variations and are one of the most diverse group of secondary metabolites. The hydroxyl (-OH) groups in phenolic compounds are thought to cause inhibitory action as these groups can interact with the cell membrane of bacteria to disrupt membrane structures and cause leakage of cellular components [14]. In fact, the use of EOs for reducing the development of plant and human pathogenic microorganisms in food, particular fruits and vegetables, requires exhaustive knowledge of their antimicrobial activity and their interactions with product components and with other antimicrobial compounds [14]. Therefore, this research work aimed to study the effectiveness of three different citrus essential oils as emulsions on the post-harvest shelf life, and nutritional quality of strawberries that could be greatly promising. This study could introduce a more effective treatment to prolong the post-harvest shelf-life of strawberries, which would benefit consumers, sectors of the agri-food industry, and the wider producers.

Fruit Samples
Fresh strawberry fruits (Fragaria × ananassa) of the variety Festival were harvested at commercial stage (75% of fruits surface with red color) from a private farm located in Cairo, Egypt (latitude 30.0131 • N, longitude 31.2089 • E, 694 m). Fruits were investigated visually for fungi contamination and physical damages. Uniform berries in appearance (color and shape) were selected for the experiment.

Essential Oils
Essential oils of the lemon, orange and mandarin were acquired from El-Marwa food industries (6th of October City, Egypt) (Juhayna group). The cultivars of citrus used to extract the essential oils were Balady for orange, Adalia for lemon and Description for mandarin. Chemical composition of the essential oils were assessed by gas chromatography-mass spectrometry (GC-MS) at National Research Center, Egypt.

Estimated Antimicrobial Activity of Lemon, Orange and Mandarin Oils
The antimicrobial activity of essential oils was determined using the disk diffusion technique against different types of microorganism such as (Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 9027, Bacillus subtilis ATCC 33221, Salmonella typhimurium ATCC 20231, Aspergillus niger NRRL 2035, Aspergillus flavus NRRL 3357, Saccharomyces cerevisiae NRRL 2034 and Candida lypolitica NRRL 1095).They were obtained from Northern Regional Laboratories, Peoria, IL, USA (NRRL strains), American Type Culture Collection, Manassa, VA, USA (ATCC strains). The strains were activated and obtained the growth in 2.5 × 10 5 cfu (colony-forming units) mL −1 number of cells. The disks of 5 mm in diameter were placed on the nutrient agar surface (Sigma chemical company, St. Louis, MO, USA) and separately fecundated with 5 µL of each essential oil (El-Marwa food industries, Egypt) using four concentrations (50, 100, 150 and 250 µL·m −1 ). The plates of mold and yeast were incubated at 28 • C for 48 h, while, the plates of bacteria were incubated for 35 • C for 48 h. Antimicrobial activity was defined as absenteeism of microbial growth in the area around the disk. The diameter of the inhibition zones was determined with a ruler [15].

Antiradical Scavenging Activity of Essential Oils
Radical scavenging procedure DPPH (radical 2,2 -diphenyl-1-picrylhydrazyl) (Sigma chemical company, St. Louis, MO, USA) was used to test the antioxidant capacity and determined the IC50 of essential oils extracted from differ citrus fruit peels (orange, mandarin and lemon) Different concentrations of essential oils were used to assess the antioxidant activity compared with ascorbic acid (IC50 (30.32 ± 0.16) µg·ml −1 ) according to Kizil et al. [16]. Different concentrations were prepared of each essential oils (50,100,150,200 and 250 µL·ml −1 methanol), then 50 µL of each concentration was diluted with 1 mL of methanol (Qualikems, Vadodara, India) and added to 5 mL of a DPPH Agronomy 2020, 10, 1466 4 of 29 methanol solution (0.004 percent) the recent mixtures were incubated for 30 min at room temperature in the dark. The absorbance of sample and control was measured at 517 nm using spectrophotometer (model UV-2401 PC, Shimadzu, Milano, Italy). Inhibition free antioxidant (%) was calculated as follows: where, A control is the absorbance of the DPPH radical solution (0.004%) + methanol, and A sample is the absorbance of the test oils antioxidant reaction. Inhibition % against concentration was plotted after 30 min, and compared to standard/commercial antioxidants (vitamin C), the inhibitory concentration (IC50) of the essential oil required to inhibit 50% of the DPPH radicals obtained from the standard curve. A lower value of IC50 indicates greater antioxidant activity. Tests were performed in triplicate.

Preparation of Emulsions for Coating
The coating forming solutions were prepared according to the method of Rojas-Graü et al. [17]. The 2% (v/v) of lemon, orange, or mandarin essential oils and Tween 80 as an emulsifier (10% of the volume of the essential oil) were dissolved into distilled water. Emulsion was stirred at 13,000 rpm for 2 min using High-performance disperser (IKA T25-Digital Ultra Turrax, Staufen, Germany) at room temperature.

Application of Emulsions to Strawberry
Sorted strawberries were submerged in the respective solutions of treatments for 4 min, corresponding to L = lemon essential oil emulsion (2%), O = orange essential oil emulsion (2%), M = mandarin essential oil emulsion (2%) and C = control (Untreated fruits) then air-dried for two hours in a sterile room. After the coating process was performed, the coated fruits of each treatment were equally packed into polyethylene terephthalate packages (dimensions of 178 × 127 × 89 mm) and stored at 2 ± 1 • C for 18 days. A total of 144 boxes were used to perform all the experiments (36 boxes/treatment). For determination of the changes in microbial communities and physiochemical properties of treated fruits, six boxes from each treatment were randomly taken after 0, 4, 7, 10, 14, and 18 days of storage and never returned back. Zero day is after 12 h after application of treatments. The whole set of experiment was carried out two times. The experimental design was completely randomized design with six replications.

Weight Loss
At the beginning of the experiment, the strawberry fruits of each treatment and replicates were separately labeled and weighed prior to storage and also every sampling date during the storage period (4, 7, 10, 14, and 18 days) for determining the weight loss. The weight loss percentage was calculated using the following Equation (2).
Weight loss percentage (WL%) = FW − IW IW × 100 (2) where, FW is the final weight and IW is the initial weight.

Decay Percentage
The methodology used to determine the decay was a modification as described by [18]. Strawberries were visually evaluated on days 4, 7, 10, 14, and 18 days, and then the weight of Agronomy 2020, 10, 1466 5 of 29 decayed strawberries was recorded. Mycelia growth, visible lesion, brown spots and softening on fruits were considered as decayed. The decay percentage was calculated using the following Equation (3).
In which, D% is the decay percentage and WIF and WAF are the weight of decayed fruits and total weight of strawberry fruits at sampling date, respectively.

Surface Color
The surface color of the strawberries was determined using a hand-held tristimulus reflectance colorimeter (model CR-400, Minolta Corp., Newburgh, NY, USA). Calibration was performed using a white plate. The measurements were performed on fruit surfaces before storage (after coating process) and also every sampling date during the storage period (0, 4, 7, 10, 14, and 18 days). To avoid the effects of heterogeneity on the fruit surface, measurements were taken in the same previously labeled most expanded shoulder of each fruit. CIE-L* (Lightness), a* (-greenness to + redness), and coordinates were recorded [19]. a* value is the main index for fruit senescence.

Fruit Firmness
The firmness of the strawberries of each treatment during the storage periods was measured using a digital force gauge (model M4-200, MARK, Copiague, NY, USA) equipped with 8 mm diameter cylindrical probe [20]. Three different locations were used to measure firmness on the most expanded region of the fruit, especially around the equatorial area. The firmness was expressed as gram per centimeter square of force (g cm −2 ). The firmness was determined after 0, 4, 7, 10, 14, and 18 days.

Soluble Solid Content (SSC)
The sample was crushed in a mortar and squeezed by hand to acquire juice. A digital refractometer (model PR101, Co. Ltd., Atago, Tokyo, Japan) was used to measure total soluble content (SSC) in fruit juices of different treatments and the SSC value was expressed as a percentage on the Brix scale. Soluble solid content was determined after 0, 4, 7, 10, 14, and 18 days.

Sensory Assessment
Sensory evaluation and general appearance was obtained by submitting samples to a trained panelist from the vegetables department, Faculty of Agriculture, Cairo University, Giza, Egypt to make evaluations. Samples were identified in descending trend with 5 being the best and 1 being the worst. Each sample was rated using score system as follows: 5 = excellent, 4 = very good, 3 = good, 2 = fair and 1 = very poor). This scale describes fresh appearance, fresh calyx, change of color and decay. Data was collected on color, texture, flavor, appearance and overall acceptability on all the sampling days. A general score of 3 was considered as the limit for consumption ability. Sensory evaluation was determined according to [21].

Respiration Rate
Respiration rate was expressed as a concentration of carbon dioxide and oxygen inside the gas jar containing the fruits. Oxygen and carbon dioxide analyzer (Model 902P, Quantek Instruments, Grafton, MA, USA) equipped with oxygen/carbon dioxide sensor PS-2110 was used to measure the respiration rate. This was done in a 175 mL hermetically sealed glass jar using two fruits. After two hours, the respiratory rate of the fruits was recorded on days 0, 4, 7, 10, 14, and 18 for each treatment, using six replications per treatment. To calculate the rate of respiration in a static system, we need to know the volume of container, weight of tissue, initial CO 2 concentration, Length of time and final CO 2 concentration. Titratable acidity (TA) was determined by using 10 g aliquots of strawberry fruits mixed in 90 mL of distilled water, then homogenized, and filtered; 10 mL of the filtrate was titrated with 0.1N NaOH (El-Nasr Company, Giza Governorate, Egypt) to an end-point (pH 8.0-8.4). TA was expressed as a percentage of citric acid and was calculated using the method reported by Association of Official Analytical Chemists (A.O.A.C.) [22]. The ascorbic acid content was assessed using a 2, 6-dichlorophenol indophenol titration method as described in A.O.A.C. [21]. 10 g of the fruit tissue was homogenized with 90 mls of oxalic acid (3%) (Nice chemical LTD, Kerala, India), the sample was then filtered using a filter paper (Whatman paper); 25 mls of the filtrate was titrated by 2, 6-dichlorophenol indophenol (Qualikems, Gujarat, India).

Total Phenolic Compounds (TPC)
The Folin-Ciocalteu method, with minor modification, was used to measure total phenolic compounds (TPC) [23], which based on the colorimetric reaction of phenols. Briefly, 100 g of strawberry fruit were homogenized and 5 g was mixed with 50 mL methanol (50%). The mixture was shaken (for 30 min at 200 ppm) and filtered through filter paper (Whatman paper). The filtrate was collected, then 0.2 mL was taken and mixed with 1 mL Folin reagent (1:10 Folin: water)(Loba Cheme, India), 0.8 mL of sodium carbonate (7.5%) (El-Nasr Company, Egypt) was added then kept in the dark for 1 h at room temperature. The absorbance was measured at 765 nm using a spectrophotometer (Model UV-2401 PC, Shimadzu, Milano, Italy). TPC was calculated as gallic acid equivalents (GAE) in mg· 100 g −1 fresh fruit.

Total Anthocyanin
For this assay, 100 g of strawberry fruit were homogenized, 4 g of the strawberry puree was dissolved in 40 mL of extraction solvent (ethanol:0.1 M HCl-85:15%, v/v) (Merk, Darmstadt, Germany) and sonicated for 10 min. After filtration the supernatant was collected and used for total anthocyanin determination by the pH differential spectroscopic method as described by [24]. Extractions were done in triplicate and the results were calculated according to following Equations (4) and (5).
where, TA: total anthocyanin mg·kg −1 fresh weight fruit; M: molecular weight value was 445; DF: the dilution factor; ź: the molar absorptivity coefficient values 26,900 M −1 cm −1 ; λ: the cuvette optical path length (1 cm) and m: the weight of the sample (g).

Antioxidant Activity
Antioxidant activity was measured by using of the free-radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH, from Sigma Company, St. Louis, MO, USA) test, with minor modification, as described by [25]. For this assay, 0.2 mL of previous extract aliquot were dissolved in 3.8 mL of a methanol DPPH solution (0.0024 g·100 mL −1 ). The mixture was shaken and kept in the dark at room temperature for 30 min. The absorbance was measured at 510 nm. The antioxidant activity was calculated as % of inhibition according to Equation (6): In which, A control and A sample are the absorbance of the control and sample, respectively.

Polyphenol Oxidase Activity
Polyphenol oxidase (PPO) was determined by spectrophotometer (Unico, UV2000, Rochester, NY, USA) using Catechol (0.2 mm L −1 ) (Qualikems, Gujarat 391340, India) as a substrate and the activity was defined as unit mg −1 ·min −1 . One unit equal the change in absorbance at 420 nm of 0.001 per minute at pH 7 and the reaction mixture at 25 • C [26].
Catalase Catalase (CAT) was determined according to the method by Aebi [27] by recording the quinoneimine dye absorbance at 510 nm of a reaction mixture containing 0.50 mL sodium phosphate buffer (pH 7.0, 0.1 M), 0.10 mL diluted H 2 O 2 (10 µL H 2 O 2 :10 mL distilled water) and 0.05 mL of sample (enzyme extract) after one min at 25 • C add 0.20 mL chromogen-inhibitor and 0.50 mL peroxidase then incubated 10 min. at 37 • C. The blank was determined using the same above steps without sample. The catalase kits were purchased from Biodiagnostic Company, Egypt. Enzyme activity was expressed as U g −1 . Unit of CAT activity is calculated using the following equation: 2.8.6. Microbiological Analysis These analyzes were performed in duplicate, for the count of coliform group bacteria, bacteria, molds and yeast on days 0, 4, 7, 10, 14 and 18 on the treatments. Briefly, 50 g of strawberries from each treatment were mixed with sterilized saline solution (450 mL, 0.85% NaCl), and then homogenized using an electric blender in aseptic conditions. Serial 10-fold dilutions were prepared in each treatment. Then 1 mL of each sample was transferred to dishes containing nutrient agar for total count bacteria, potato dextrose agar (PDA) for mold and yeast and eosine methylene blue agar (EMBA) for total coliform group determination. Thereafter, plates for total count bacteria and mold and yeast were incubated at 28 • C for 48 h, while plates for coliform bacteria (coliform group) were incubated at 35 • C for 48 h. The experiment was performed using two replicate counts, and results were presented in Log cfu g −1 [28]. (All the medium of agar were purchased from the Sigma chemical company, St. Louis, MO, USA)

Statistical Analysis
One way analysis of variance (ANOVA) and the least significant difference method (LSD) for mean separation, with a confidence level of 95%, were used in this study to assess the impact of the different treatments at each storage period (0, 4, 7, 10, 14 and 18) on the studied variables of strawberries. The results were represented as means ± standard deviations and ANOVA was performed by using the M.Stat program (Version 2.1, Michigan state university, East Lansing, MI, USA). Pearson correlation was performed by using SPSS program (Inc., Chicago, IL, USA, version, 14) to understand the relationships between the total antioxidant content of tread fruit with essential oils and different physicochemical additionally microbiological properties of fruits.  Figure 1 demonstrates the radical scavenging activity of methanol extracts of different citrus fruit peels as the percent inhibition of DPPH. Methanolic extracts were shown to have antioxidant activity, and essential oils had radical scavenging effects at all concentrations. However this effect was not as prominent as the standard ascorbic acid's radical scavenging effect. The IC50 values for the different extracts were 229.2, 208.9 and 200 µL·ml −1 , compared to ascorbic acid IC50 (30.61 µL·ml −1 ) for the orange, lemon and mandarin oils, respectively. IC50, the median inhibitory concentration, was determined from the graph, which was calculated by plotting inhibition percentages (50%) against concentrations (200 µL·ml −1 ) of mandarin oil as an example. The essential oils studied included orange oil which possessed the weakest radical scavenging activity. Meanwhile, at all concentrations the mandarin essential oil was assayed with the strongest radical-scavenging effect. Citrus essential oils in terms of antioxidant may cause free radical scavenger limonene activity [29]. The major constituents of the essential oils were as follows: mandarin: D-limonene (93-96%); lemon: limonene (59-80%) and orange: limonene (85-96%) were presented in Table 1. Our findings were in line with [30][31][32]. Nonetheless, essential oils' antioxidant activities can differ depending on the variations in chemical composition. An essential oil's antioxidant activity is mainly attributed to its major components, although consideration must be given to the synergistic or antagonistic effect of one compound in a minor percentage of the mixture [33].

Antiradical Scavenging Activity of Essential Oils and their Components
Agronomy 2020, 10, x FOR PEER REVIEW 8 of 29

Chemical and Antimicrobial Characterization of Essential Oils
3.1.1. Antiradical Scavenging Activity of Essential Oils and their Components Figure 1 demonstrates the radical scavenging activity of methanol extracts of different citrus fruit peels as the percent inhibition of DPPH. Methanolic extracts were shown to have antioxidant activity, and essential oils had radical scavenging effects at all concentrations. However this effect was not as prominent as the standard ascorbic acid's radical scavenging effect. The IC50 values for the different extracts were 229.2, 208.9 and 200 μL·ml −1 , compared to ascorbic acid IC50 (30.61 μL·ml −1 ) for the orange, lemon and mandarin oils, respectively. IC50, the median inhibitory concentration, was determined from the graph, which was calculated by plotting inhibition percentages (50%) against concentrations (200 μL·ml −1 ) of mandarin oil as an example. The essential oils studied included orange oil which possessed the weakest radical scavenging activity. Meanwhile, at all concentrations the mandarin essential oil was assayed with the strongest radical-scavenging effect. Citrus essential oils in terms of antioxidant may cause free radical scavenger limonene activity [29]. The major constituents of the essential oils were as follows: mandarin: D-limonene (93-96%); lemon: limonene (59-80%) and orange: limonene (85-96%) were presented in Table 1. Our findings were in line with [30][31][32]. Nonetheless, essential oils' antioxidant activities can differ depending on the variations in chemical composition. An essential oil's antioxidant activity is mainly attributed to its major components, although consideration must be given to the synergistic or antagonistic effect of one compound in a minor percentage of the mixture [33].

Antimicrobial Activity of Essential Oils
The results of estimating the antimicrobial activity of the essential oils of lemon, orange and mandarin were studied on the growth of different microbial groups (bacteria-fungi and yeast) using different concentrations (50-100-150 and 200 µL) of the aforementioned essential oils ( Table 2). The higher antimicrobial activity observed for lemon with the different concentrations was used, where it showed a positive effect on all tested strains, except for the Bacillus subtilis ATCC 33221 strain, followed by lower antimicrobial activity for orange and mandarin EOs. Orange oil was showed an effect on all strains, including Bacillus subtilis ATCC 33221, but the inhibition zone area was less than the lemon oil. Finally, the mandarin oil showed the least effect on the tested strains.

Weight Loss
The weight loss of strawberries is mainly correlated to the respiration rate and evaporation of moisture through the fruit skins. Furthermore, the rapid loss of water from the fruit skin is considered one of the important factors that participate in the perishability of strawberries [34]. This drives the dryness of fruits and eventually shrinkage and deterioration. As shown in Figure 2, insignificant differences were observed among fruit samples covered with essential oil coatings (lemon, orange, and mandarin). However, a highly significant difference was found between treated and untreated strawberry samples at the end of storage periods (p = 0.008 ***). This reduction in weight loss rate in coated fruits, during storages, was due to barrier property and antioxidant activity of essential oils coatings [2,4,34]. This type of coatings might be able to decrease the gas and water exchange between fruit surfaces and the surrounding environment resulting in delaying the respiration rate, water loss and oxidation of metabolic compounds [34][35][36]. Similar findings were reported by Sánchez-González et al. [37] who found that a significant reduction of weight loss was achieved when essential oils were added into edible coatings than using the edible coatings alone.

Decay Percentage
The decay percentage of strawberry samples during the storage period is presented in Figure 3. The signs of fungal decay were observed in untreated fruits at day 7 of the cold storage and increased significantly with increasing the period of storage and recorded the highest value at the end of the storage period (at day18). Signs of fungal decay were observed in treated fruits with orange and lemon oils at day 10 of cold storage and in treated fruit with mandarin oil at day 14 of cold storage. All treated fruits with essential oils, at the end of storage periods showed lower decay percentage compared with untreated strawberry (p = 0.034 **). There was not a significant difference in decay percentage among treated fruits with different essential oils. This finding indicated that the treatment with essential oils was effective to reduce fruit spoilage by inhibition of the fungal growth on the fruit surfaces. This might be attributed to antimicrobial compounds found in applied essential oils and caused damage for cell membranes of fungi [4,15,16]. In addition to their effectiveness in reducing the respiration rate and water loss, which kept the strawberries under high carbon dioxide concentration (CO2) and low-moisture content, thus not supporting the growth of fungi. This result was in accordance with several scientists who reported that the treated fruit samples with limonene, cinnamon, oregano, bitter orange, and mandarin essential oils were lower prone to spoil and deteriorate than untreated fruits [34,[38][39][40].

Decay Percentage
The decay percentage of strawberry samples during the storage period is presented in Figure 3. The signs of fungal decay were observed in untreated fruits at day 7 of the cold storage and increased significantly with increasing the period of storage and recorded the highest value at the end of the storage period (at day18). Signs of fungal decay were observed in treated fruits with orange and lemon oils at day 10 of cold storage and in treated fruit with mandarin oil at day 14 of cold storage. All treated fruits with essential oils, at the end of storage periods showed lower decay percentage compared with untreated strawberry (p = 0.034 **). There was not a significant difference in decay percentage among treated fruits with different essential oils. This finding indicated that the treatment with essential oils was effective to reduce fruit spoilage by inhibition of the fungal growth on the fruit surfaces. This might be attributed to antimicrobial compounds found in applied essential oils and caused damage for cell membranes of fungi [4,15,16]. In addition to their effectiveness in reducing the respiration rate and water loss, which kept the strawberries under high carbon dioxide concentration (CO 2 ) and low-moisture content, thus not supporting the growth of fungi. This result was in accordance with several scientists who reported that the treated fruit samples with limonene, cinnamon, oregano, bitter orange, and mandarin essential oils were lower prone to spoil and deteriorate than untreated fruits [34,[38][39][40].

Firmness
Fruit firmness is one of the most important quality attributes for strawberries. The change in the firmness of strawberry fruits treated with essential oils is shown in Figure 4. During the storage period, the firmness values of all stored samples decreased with increasing storage periods. At the end of storage periods, after 18 days, the lowest value of firmness was recorded in the untreated strawberry fruits than treated fruits with mandarin essential oil (p = 0.192). Several authors reported that the tissue of untreated strawberry fruits becomes soft due to loss of cell wall structure through increasing the enzyme activity, which results in diminishing the hardiness of fruits during the preservation period [4]. On the other hand, the maximum values of firmness were recorded in treated fruits with mandarin oil followed by lemon and orange oils compared to untreated fruits. Similar results were observed by Severino et al. [41] who reported that the firmness and the other quality attributes of treated fruits with mandarin and lemongrass oils were maintained for 15 days of cold storage. This probably was due to selective permeability of coatings material to gas and water transmission, thus reducing respiration rates, enzyme activities, and most of the metabolic changes, and thereby delaying ripening and over softening of strawberry fruits [42].

Firmness
Fruit firmness is one of the most important quality attributes for strawberries. The change in the firmness of strawberry fruits treated with essential oils is shown in Figure 4. During the storage period, the firmness values of all stored samples decreased with increasing storage periods. At the end of storage periods, after 18 days, the lowest value of firmness was recorded in the untreated strawberry fruits than treated fruits with mandarin essential oil (p = 0.192). Several authors reported that the tissue of untreated strawberry fruits becomes soft due to loss of cell wall structure through increasing the enzyme activity, which results in diminishing the hardiness of fruits during the preservation period [4]. On the other hand, the maximum values of firmness were recorded in treated fruits with mandarin oil followed by lemon and orange oils compared to untreated fruits. Similar results were observed by Severino et al. [41] who reported that the firmness and the other quality attributes of treated fruits with mandarin and lemongrass oils were maintained for 15 days of cold storage. This probably was due to selective permeability of coatings material to gas and water transmission, thus reducing respiration rates, enzyme activities, and most of the metabolic changes, and thereby delaying ripening and over softening of strawberry fruits [42].

Soluble Solid Content (SSC)
The change in soluble solids content (SSC) of strawberry during the storage period is shown in Figure 5. Generally, the SSC values of all strawberry samples gradually decreased with increasing storage period. No significant changes were observed in SSC content among all coating treatments. After 18 days of storage, the maximum values of SSC were observed in uncoated fruits while the lowest values were recorded in treated fruits (p = 0.0054 **). The increase in the SSC value of untreated fruits stored at 2 °C might be due to rapid water loss from the fruit surface [26]. Conversely, the reduction in SCC values of coated fruits was probably associated with slow water loss from fruit surface, this was due to the ability of coating material to reduce the migration of water from the fruit surface to the surrounding environment [4].

Soluble Solid Content (SSC)
The change in soluble solids content (SSC) of strawberry during the storage period is shown in Figure 5. Generally, the SSC values of all strawberry samples gradually decreased with increasing storage period. No significant changes were observed in SSC content among all coating treatments. After 18 days of storage, the maximum values of SSC were observed in uncoated fruits while the lowest values were recorded in treated fruits (p = 0.0054 **). The increase in the SSC value of untreated fruits stored at 2 • C might be due to rapid water loss from the fruit surface [26]. Conversely, the reduction in SCC values of coated fruits was probably associated with slow water loss from fruit surface, this was due to the ability of coating material to reduce the migration of water from the fruit surface to the surrounding environment [4].

Surface Color
Color is considered one of the most important quality parameters for strawberry fruits. The red color in strawberry fruits is more associated with the accumulation of anthocyanin in the ripe fruits [34]. This pigment also is largely affected by preservation conditions, in particular, temperature and the respiration rate (the CO2 concentration), as reported by Jin et al. [43]. The surface color of strawberries was determined by recording the lightness value (L*) and redness value (a*), as shown in Figure 6. The value of lightness (L*) and redness (a*) of all strawberry fruits was affected by increasing the storage period. During the storage periods, the strawberry fruit treated with essential oils resulted in a lighter color (high L* values) while untreated fruit had dark color (lower L* values), as shown in Figure 6A. By contrast, the redness values (a*) of all fruits were increased with increasing storage periods for all treatments, but the treated fruits had less redness (Low a* value) than untreated fruits (high a* value) without any significant treatment between then until 14 days, as illustrated in Figure 6B. In general, at the end of the storage (18 days), the colors of the treated fruits were slightly light red and of untreated fruits dark red, thus indicating the effectiveness of essential oil coatings in

Surface Color
Color is considered one of the most important quality parameters for strawberry fruits. The red color in strawberry fruits is more associated with the accumulation of anthocyanin in the ripe fruits [34]. This pigment also is largely affected by preservation conditions, in particular, temperature and the respiration rate (the CO 2 concentration), as reported by Jin et al. [43]. The surface color of strawberries was determined by recording the lightness value (L*) and redness value (a*), as shown in Figure 6. The value of lightness (L*) and redness (a*) of all strawberry fruits was affected by increasing the storage period. During the storage periods, the strawberry fruit treated with essential oils resulted in a lighter color (high L* values) while untreated fruit had dark color (lower L* values), as shown in Figure 6A. By contrast, the redness values (a*) of all fruits were increased with increasing storage periods for all treatments, but the treated fruits had less redness (Low a* value) than untreated fruits (high a* value) without any significant treatment between then until 14 days, as illustrated in Figure 6B. In general, at the end of the storage (18 days), the colors of the treated fruits were slightly light red and of untreated fruits dark red, thus indicating the effectiveness of essential oil coatings in decreasing the anthocyanin accumulation and delaying the senescence of fruits (p = 0.046 * for L* value and 0.0919 for a* value). This could be related to the capability of coatings formulated from essential oils to increase the internal CO 2 concentration surrounding fruits which able to reduce the respiration rate ( Figure 7) and delay the accumulation of the anthocyanin pigments in treated strawberries (Figure 8). These findings were in agreement with those reported by Jin et al. [43], who found that treatment with CO 2 significantly reduced the accumulation of anthocyanin and changed significantly the color of stored fruit.
Agronomy 2020, 10, x FOR PEER REVIEW 14 of 29 decreasing the anthocyanin accumulation and delaying the senescence of fruits (p = 0.046 * for L* value and 0.0919 for a* value). This could be related to the capability of coatings formulated from essential oils to increase the internal CO2 concentration surrounding fruits which able to reduce the respiration rate ( Figure 7) and delay the accumulation of the anthocyanin pigments in treated strawberries (Figure 8). These findings were in agreement with those reported by Jin et al. [43], who found that treatment with CO2 significantly reduced the accumulation of anthocyanin and changed significantly the color of stored fruit.

Respiration Rate
Perishable fruits, such as strawberries, are considered as having a high respiration rate and are severely affected by time and temperature of storage [2,36]. Figure 7 shows the level of the respiration rate, expressed as mg of CO 2 kg −1 ·h −1 , on days 0, 4, 7, 10, 14, and 18. The respiration rate of all samples was increased by increasing the storage period. At the end of the storage period, the lowest rates of the respiration rate were recorded in the treated fruits with different essential oils while the highest rates were observed in the untreated fruits (p = 0.006 **). No significant differences were observed in the respiration rates among the fruits treated with different essential oils (lemon, orange and mandarin). These results indicate that the treatment with different essential oils caused a significant reduction in the respiration rate. The findings suggest that application of essential oils coatings significantly reduced the respiration rate of fruits in a similar method to storage under a controlled atmosphere. This was due to an antioxidant activity and the gas permeability of used essential oils covered the fruit surface that could have changed the endogenous content of CO 2 and O 2 gas, thus reducing the respiration rate of fruits [4]. Similar results were reported by Marín et al. [44], who found that the use of lemon essential oil with chitosan significantly decreased the respiration rate compared to the use of chitosan coating alone after 12 days of storage.
Natural antimicrobial edible coatings are widely used in fresh and minimally processed fruits and vegetable, to decrease respiration, reduce loss of flavor compounds, and extend the self-life of fruits and retard the maturation process [4]. Nevertheless, the change in the internal gas composition due to the application of coating film can produce alcoholic flavors as a result of anaerobic fermentation linked to the accumulation of carbon dioxide and reduction of oxygen content [45]. For that reason, it is required to control the alcoholic flavors in sensorial panels. In our study, there were no off-odors and off-tastes produced in the coated fruits, during the storage periods. The efficiency of edible films in conserving the quality of fresh vegetables is strongly associated with the use of a proper coating material that is able to introduce a desirable internal gas composition to reduce the transpiration rates of fruits without production of the fermentative compounds that contribute to the development of off-odors and off-tastes [4,44].

Total Anthocyanin Content
Anthocyanins belongs to the polyphenol groups and it is responsible for the red color in the ripe strawberry fruits. A regulatory enzyme, the phenylalanine ammonia-lyase is accountable for the biosynthesis of anthocyanin in fruits and vegetables [46]. Generally, the concentrations of total phenols and total anthocyanins in the fruits depend on varieties and the maturity stage of strawberries at harvest in the open field or during storage. The results pointed out that the untreated fruit showed the maximum value of anthocyanin concentration (253.97 mg·kg −1 ) at the end of the storage, compared to 6.82 mg·kg −1 as the storage began ( Figure 8). The significant increase, especially in control, could be explained as a natural process during fruit ripening, in addition to the effect of the high weight loss (Figure 2) that might have contributed to the concentration of pigments. However, the treated fruits with essential oils showed a lower concentration of anthocyanin than the untreated ones. Furthermore, significant differences were found in total anthocyanin among coated fruits with essential oil types. The lowest concentration of total anthocyanin content was recorded in treated fruits with mandarin oil followed by lemon and orange oils compared with untreated fruits (p = 0.0005 ***). This result confirmed what was previously shown in Figure 6, that the color of treated fruits with essential oils were lower darker and redder than untreated fruits. This was due to delaying the synthesis of anthocyanin and the other pigments contributing to the red color in strawberries [47]. Similar results have been observed in different fruits treated with coatings based on chitosan [26]. Moreover, the better findings were achieved when essential oils were added with those edible coatings [48]. The reduction in anthocyanin concentration in treated fruit could be linked to the antioxidant property of the essential oils that reduced diffusion of oxygen and increased the CO 2 accumulation around the strawberry surfaces, and thus consequently reduced the delayed activity of enzymes and biochemical reactions responsible for anthocyanin biosynthesis [49]. This result was observed by Pelayo et al. [50], who found that the level of anthocyanin in the stored strawberries was inhibited due to increasing the concentration of CO 2 . Moreover, the treatment with essential oils significantly reduced the respiration rate ( Figure 7) which consequently reduced the water loss from the fruit surface and this explained the reduction of anthocyanin concentration in coated fruits when compared with uncoated fruits. Therefore, treatments with essential oils can effectively delay the fruit ripening and extend the post-harvest self-life of strawberries.

Total Phenolic Compounds (TPC)
Total phenols are quite important compounds for the quality of strawberry fruits, not only due to their involvement with anthocyanins in the flavor and the color of most fruits and vegetables [51] but also their involvement in the preservation of fruit quality during storage. Polyphenol compounds possess antimicrobial, anti-aging and antioxidant properties, making these compounds an important indicator to evaluate the fruit and vegetable conservation effect [52]. Changes in the total phenolic content of all the stored strawberries are presented in Figure 9. The trends of total phenols content in all samples increased gradually after storage began and peaked on Day 10, then the trend slowed down gradually and peaked on Day 18 (p = 0.0035 **). The fast accumulation of total phenols content in all samples in the first ten days could be linked to an increase in the accumulation of other phenolic compounds [53].
Agronomy 2020, 10, x FOR PEER REVIEW 17 of 29 inhibited due to increasing the concentration of CO2. Moreover, the treatment with essential oils significantly reduced the respiration rate ( Figure 7) which consequently reduced the water loss from the fruit surface and this explained the reduction of anthocyanin concentration in coated fruits when compared with uncoated fruits. Therefore, treatments with essential oils can effectively delay the fruit ripening and extend the post-harvest self-life of strawberries.

Total Phenolic Compounds (TPC)
Total phenols are quite important compounds for the quality of strawberry fruits, not only due to their involvement with anthocyanins in the flavor and the color of most fruits and vegetables [51] but also their involvement in the preservation of fruit quality during storage. Polyphenol compounds possess antimicrobial, anti-aging and antioxidant properties, making these compounds an important indicator to evaluate the fruit and vegetable conservation effect [52]. Changes in the total phenolic content of all the stored strawberries are presented in Figure 9. The trends of total phenols content in all samples increased gradually after storage began and peaked on Day 10, then the trend slowed down gradually and peaked on Day 18 (p = 0.0035 **). The fast accumulation of total phenols content in all samples in the first ten days could be linked to an increase in the accumulation of other phenolic compounds [53]. Shin et al. [54] stated that differences in the total phenolic content of strawberry fruits during storage were affected by the maturity of fruits at the harvest and storage period. At the end of storage periods, the highest content of total phenols content was recorded in coated fruits treated with mandarin essential oil followed by lemon oil and orange oil compared with uncoated fruits. This might be associated with the antioxidant properties of applied essential oils that reduced the oxidation of phenolic compounds [55]. These results were in agreement with those obtained by Gol et al. [49] who found that the content of phenolic compounds was significantly increased in coated fruit treated with chitosan incorporated with essential oils than uncoated fruits. On the other hand, Shin et al. [54] stated that differences in the total phenolic content of strawberry fruits during storage were affected by the maturity of fruits at the harvest and storage period. At the end of storage periods, the highest content of total phenols content was recorded in coated fruits treated with mandarin essential oil followed by lemon oil and orange oil compared with uncoated fruits. This might be associated with the antioxidant properties of applied essential oils that reduced the oxidation of phenolic compounds [55]. These results were in agreement with those obtained by Gol et al. [49] who found that the content of phenolic compounds was significantly increased in coated fruit treated with chitosan incorporated with essential oils than uncoated fruits. On the other hand, the decline of phenolic content, at the end of the storage period, in uncoated strawberries could be due to continuous respiration rate and the breakdown of cell structure [36].

Titratable Acidity
The amount of titratable acidity (TA) in the strawberry is directly correlated to the content of organic acids existing in the fruits. The changes in the amount of TA of strawberry during storage are shown in Figure 10. The amount of TA of strawberries during storage decreased gradually with the extension of the storage periods. At the end of the storage periods, the amount of TA in the coated fruits was greater than that of the uncoated fruits, indicating that edible coatings with essential oils are favorable to maintain the level of TA in the fruits during preservation (p = 0.0466 *). Similar findings were observed by Dhital et al. [34] and Gol et al. [49]. They found that the edible coatings incorporated with essential oils reduced the water loss, respiration rate, and microbial growth which consequently decreased the consumption of organic acids (i.e., titratable acid) in the respiratory metabolic activities of strawberry fruits. This efficiently reduces the loss of titratable acid, extending the post-harvest shelf life of stored fruits.
Agronomy 2020, 10, x FOR PEER REVIEW 18 of 29 the decline of phenolic content, at the end of the storage period, in uncoated strawberries could be due to continuous respiration rate and the breakdown of cell structure [36].

Titratable Acidity
The amount of titratable acidity (TA) in the strawberry is directly correlated to the content of organic acids existing in the fruits. The changes in the amount of TA of strawberry during storage are shown in Figure 10. The amount of TA of strawberries during storage decreased gradually with the extension of the storage periods. At the end of the storage periods, the amount of TA in the coated fruits was greater than that of the uncoated fruits, indicating that edible coatings with essential oils are favorable to maintain the level of TA in the fruits during preservation (p = 0.0466 *). Similar findings were observed by Dhital et al. [34] and Gol et al. [49]. They found that the edible coatings incorporated with essential oils reduced the water loss, respiration rate, and microbial growth which consequently decreased the consumption of organic acids (i.e., titratable acid) in the respiratory metabolic activities of strawberry fruits. This efficiently reduces the loss of titratable acid, extending the post-harvest shelf life of stored fruits.

Ascorbic Acid
Ascorbic acid plays an important role in fruit preservation during storage [36]. Change in ascorbic acid concentration of strawberries during storage is presented in Figure 11. The total ascorbic acids of all coated and uncoated samples of the strawberry fruits significantly decreased with increases in the storage periods. This reduction could be related to its oxidation through superoxide and hydroxyl radicals in the strawberry fruits [2,36]. At the end of the storage period, the concentrations of ascorbic acid were higher in coated fruits with different essential oils than the uncoated fruits (p = 0.057). Significant differences in ascorbic acids were found among the treated fruits with different essential oils. The maximum values of vitamin C were observed in the strawberry fruits treated with mandarin essential oil followed by lemon and orange essential oils compared with untreated fruits (control). The result of this study suggested that the treatment with essential oils can

Ascorbic Acid
Ascorbic acid plays an important role in fruit preservation during storage [36]. Change in ascorbic acid concentration of strawberries during storage is presented in Figure 11. The total ascorbic acids of all coated and uncoated samples of the strawberry fruits significantly decreased with increases in the storage periods. This reduction could be related to its oxidation through superoxide and hydroxyl radicals in the strawberry fruits [2,36]. At the end of the storage period, the concentrations of ascorbic acid were higher in coated fruits with different essential oils than the uncoated fruits (p = 0.057).
Significant differences in ascorbic acids were found among the treated fruits with different essential oils. The maximum values of vitamin C were observed in the strawberry fruits treated with mandarin essential oil followed by lemon and orange essential oils compared with untreated fruits (control). The result of this study suggested that the treatment with essential oils can effectively reduce the loss of vitamin C (ascorbic acid) in coated strawberry fruits during storage. This might be attributed to the antioxidant properties of essential oil coatings (Figure 1) which reduced the diffusion of oxygen, decreased the rate of respiration, as shown above in Figure 7, and consequently reduced the ascorbic acid oxidation [4,44].
Agronomy 2020, 10, x FOR PEER REVIEW 19 of 29 effectively reduce the loss of vitamin C (ascorbic acid) in coated strawberry fruits during storage. This might be attributed to the antioxidant properties of essential oil coatings (Figure 1) which reduced the diffusion of oxygen, decreased the rate of respiration, as shown above in Figure 7, and consequently reduced the ascorbic acid oxidation [4,44].

Antioxidant Capacity
Antioxidant capacity is a very important factor in assessing fruits and vegetables. Several techniques are used to evaluate the total antioxidant ability, including the oxygen radical absorbance capacity (ORAC) technique, the 1-diphenyl-2-picrylhydrazyl (DPPH) technique, the 2,2-azino-bis (3ethylbenzothiazoline-6-sulfonic acid, ABTS) technique, the ferric reduction antioxidant (FRAP) technique and so on. The DPPH technique has been extensively used to determine the antioxidant capacity of foods, especially fruits and vegetables, and their byproducts due to their sensitivity, speediness, reliability, and simple technique [56].
The change in the antioxidant ratio of strawberry samples treated with essential oils during the storage periods is shown in Figure 12. The antioxidant content of the treated fruits with different essential oils significantly increased with an increase in the storage periods until 14 days of storage. After that, the total antioxidant content of the treated and untreated strawberries was decreased. However, the reduction in the antioxidant content in treated fruit was lower than the untreated fruit.
The maximum values of antioxidants were recorded in fruits treated with mandarin oil followed by lemon and orange oils compared with untreated fruits (p = 0.0006 ***). The increase of the total antioxidant capacity of treated fruits with essential oil might be associated with the relative amounts of antioxidant compounds added to strawberries after coating with essential oils, especially polyphenol compounds [2,57]. Moreover, these types of essential oils have the ability to retard the loss of other antioxidant compounds of the coated fruits such as total phenols, ascorbic acid, and anthocyanin contents, as previously mentioned (Figures 8, 9 and 11). Meanwhile, the reduction of

Antioxidant Capacity
Antioxidant capacity is a very important factor in assessing fruits and vegetables. Several techniques are used to evaluate the total antioxidant ability, including the oxygen radical absorbance capacity (ORAC) technique, the 1-diphenyl-2-picrylhydrazyl (DPPH) technique, the 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid, ABTS) technique, the ferric reduction antioxidant (FRAP) technique and so on. The DPPH technique has been extensively used to determine the antioxidant capacity of foods, especially fruits and vegetables, and their byproducts due to their sensitivity, speediness, reliability, and simple technique [56].
The change in the antioxidant ratio of strawberry samples treated with essential oils during the storage periods is shown in Figure 12. The antioxidant content of the treated fruits with different essential oils significantly increased with an increase in the storage periods until 14 days of storage. After that, the total antioxidant content of the treated and untreated strawberries was decreased. However, the reduction in the antioxidant content in treated fruit was lower than the untreated fruit.
The maximum values of antioxidants were recorded in fruits treated with mandarin oil followed by lemon and orange oils compared with untreated fruits (p = 0.0006 ***). The increase of the total antioxidant capacity of treated fruits with essential oil might be associated with the relative amounts of antioxidant compounds added to strawberries after coating with essential oils, especially polyphenol compounds [2,57]. Moreover, these types of essential oils have the ability to retard the loss of other antioxidant compounds of the coated fruits such as total phenols, ascorbic acid, and anthocyanin contents, as previously mentioned (Figures 8, 9 and 11). Meanwhile, the reduction of antioxidant capacity in untreated fruits at the end of the storage period might be due to senescence [58]. The findings of this study were consistent with the results of Martínez et al. [2] who found that edible chitosan coatings incorporated with Thymus capitatus essential oil significantly improved the total antioxidant capacity of coated strawberries over uncoated fruits.
Agronomy 2020, 10, x FOR PEER REVIEW 20 of 29 antioxidant capacity in untreated fruits at the end of the storage period might be due to senescence [58]. The findings of this study were consistent with the results of Martínez et al. [2] who found that edible chitosan coatings incorporated with Thymus capitatus essential oil significantly improved the total antioxidant capacity of coated strawberries over uncoated fruits.

Antioxidant Enzymes (Catalase and Polyphenol Oxidase)
Catalase (CAT) is considered one of the most important antioxidant enzymes in protecting the plant cell from oxidative damage by reactive oxygen species [59]. The activity of this enzyme usually increases during fruit maturation [60]. As shown in Figure 13, the activity of the CAT enzyme in all fruit samples rapidly decreased with increasing storage periods, but the reduction in the coated fruits with different essential oils was lower than uncoated fruits, at the end of the storage period (p = 0.0001 ***).

Antioxidant Enzymes (Catalase and Polyphenol Oxidase)
Catalase (CAT) is considered one of the most important antioxidant enzymes in protecting the plant cell from oxidative damage by reactive oxygen species [59]. The activity of this enzyme usually increases during fruit maturation [60]. As shown in Figure 13, the activity of the CAT enzyme in all fruit samples rapidly decreased with increasing storage periods, but the reduction in the coated fruits with different essential oils was lower than uncoated fruits, at the end of the storage period (p = 0.0001 ***). The highest CAT activity was observed in fruits treated with mandarin essential oil followed by lemon and orange than uncoated fruits. The polyphenol oxidase (PPO) enzyme is responsible for browning reaction, mainly occurs from the oxidation of phenolic substances, and leads to quality loss [61]. PPO activity in all fruits increased with the extension of storage periods, and PPO activity in coated fruits with different essential oils showed a lower level compared to uncoated fruits (p = 0.0004 ***). At the end of storage, the lowest PPO activity was in strawberries treated with mandarin oils followed by lemon and orange oils ( Figure 14). Similar findings were reported by Badawy et al. [26] who observed that the use of essential oils containing thymol (0.02%) or geraniol (0.04%) increased CAT activity and reduced PPO activity. These results suggested that the use of essential oils improves the levels of oxyradical detoxification enzymes including CAT and declines the PPO activity that prevents the oxidation damages, thus promoting prolongation of the shelf-life and preserving the quality of strawberries during the storage. The highest CAT activity was observed in fruits treated with mandarin essential oil followed by lemon and orange than uncoated fruits. The polyphenol oxidase (PPO) enzyme is responsible for browning reaction, mainly occurs from the oxidation of phenolic substances, and leads to quality loss [61]. PPO activity in all fruits increased with the extension of storage periods, and PPO activity in coated fruits with different essential oils showed a lower level compared to uncoated fruits (p = 0.0004 ***). At the end of storage, the lowest PPO activity was in strawberries treated with mandarin oils followed by lemon and orange oils ( Figure 14). Similar findings were reported by Badawy et al. [26] who observed that the use of essential oils containing thymol (0.02%) or geraniol (0.04%) increased CAT activity and reduced PPO activity. These results suggested that the use of essential oils improves the levels of oxyradical detoxification enzymes including CAT and declines the PPO activity that prevents the oxidation damages, thus promoting prolongation of the shelf-life and preserving the quality of strawberries during the storage.

Sensory Assessment
The sensory assessment of the treated strawberries is necessary since it shows the acceptability on the part of the final consumer of the fruits treated with the essential oils [62]. The findings of the hedonic evaluations are presented in Table 3 during the 18 days of storage. Results indicated that the strawberries treated with different essential oils (L, O, and M) had higher acceptance regarding color, texture, flavor, and appearance attributes than untreated strawberries. Furthermore, significant differences were found between treated fruits with different essential oils in sensory attributes, especially in color, appearance, and general acceptance at the end of the experiment.

Sensory Assessment
The sensory assessment of the treated strawberries is necessary since it shows the acceptability on the part of the final consumer of the fruits treated with the essential oils [62]. The findings of the hedonic evaluations are presented in Table 3 during the 18 days of storage. Results indicated that the strawberries treated with different essential oils (L, O, and M) had higher acceptance regarding color, texture, flavor, and appearance attributes than untreated strawberries. Furthermore, significant differences were found between treated fruits with different essential oils in sensory attributes, especially in color, appearance, and general acceptance at the end of the experiment. Table 3. Effect of different essential oil emulsions (L = lemon, O = orange, and M = mandarin essential oil) on color, texture, appearance of strawberry fruits stored at 2 °C for 18 days. Different letters indicates significant difference between treated and control fruits at each storage period (Duncan's of 95%).  Vertical bars indicate the standard deviation (n = 6). Means and different letters indicates significant difference between treated and control fruits at each storage period (Duncan's of 95%). Table 3. Effect of different essential oil emulsions (L = lemon, O = orange, and M = mandarin essential oil) on color, texture, appearance of strawberry fruits stored at 2 • C for 18 days. Different letters indicates significant difference between treated and control fruits at each storage period (Duncan's of 95%).

Treatments
Storage At the end of storage, the analysis of color was higher in strawberry fruits treated with mandarin than those treated with lemon and orange oils. The appearance of fruits treated with lemon essential oil was slightly higher than the fruits treated with mandarin and orange essential oils. There were no significant changes among the treated fruits in texture and flavor. Regarding analysis of general acceptance, all the treated fruits with different essential oils showed a higher acceptability than untreated fruits at the end of storage. This behavior indicates that the treatments with essential oils improve the organoleptic characteristics and physicochemical attributes of the strawberries [4,63]. The fruits treated with orange essential oil (O) had a lower acceptance of the sensory attributes than mandarin and lemon essential oils, but these fruits had higher acceptability of sensory properties than untreated fruits. This result might be due to rapid changes in metabolic compounds of untreated fruits (C) and then treated fruits with essential oil of orange (O) that finally affect organoleptic characteristics of strawberry fruits. Therefore, future studies should be conducted to correct the concentration of lemon essential oil can facilitate its acceptance without affecting negatively on organoleptic properties of strawberry fruits.

Microbial Study
The changes in contamination with molds, total count and coliform group on coated strawberry samples are presented in Figures 15-17. The results of the samples treated with essential oil showed a strong effect on the total count of microbes, which was reflected in the preservation period. The treatments of lemon and mandarin recorded numbers less than log 1 until the end of the storage period, while treatment of orange recorded log 3.38 at the end of storage (p = 0.002 **). The control sample reached the number of log 6.86 at the end of storage. At the end of storage, the analysis of color was higher in strawberry fruits treated with mandarin than those treated with lemon and orange oils. The appearance of fruits treated with lemon essential oil was slightly higher than the fruits treated with mandarin and orange essential oils. There were no significant changes among the treated fruits in texture and flavor. Regarding analysis of general acceptance, all the treated fruits with different essential oils showed a higher acceptability than untreated fruits at the end of storage. This behavior indicates that the treatments with essential oils improve the organoleptic characteristics and physicochemical attributes of the strawberries [4,63]. The fruits treated with orange essential oil (O) had a lower acceptance of the sensory attributes than mandarin and lemon essential oils, but these fruits had higher acceptability of sensory properties than untreated fruits. This result might be due to rapid changes in metabolic compounds of untreated fruits (C) and then treated fruits with essential oil of orange (O) that finally affect organoleptic characteristics of strawberry fruits. Therefore, future studies should be conducted to correct the concentration of lemon essential oil can facilitate its acceptance without affecting negatively on organoleptic properties of strawberry fruits.

Microbial Study
The changes in contamination with molds, total count and coliform group on coated strawberry samples are presented in Figures 15-17. The results of the samples treated with essential oil showed a strong effect on the total count of microbes, which was reflected in the preservation period. The treatments of lemon and mandarin recorded numbers less than log 1 until the end of the storage period, while treatment of orange recorded log 3.38 at the end of storage (p = 0.002 **). The control sample reached the number of log 6.86 at the end of storage. indicate the standard deviation (n = 6). Means and different letters indicate significant difference between treated and control fruits at each storage period (Duncan's of 95%).  indicate the standard deviation (n = 6). Means and different letters indicate significant difference between treated and control fruits at each storage period (Duncan's of 95%).  In the case of estimating the numbers of mold and yeast, the results showed that treatment with mandarin essential oil is the best, as no more than one logarithm was recorded during storage, except for the last sample at storage end, log 2.22, while treatments of lemon or orange essential oil were recorded by the end of storage following log 3.62 and log 3.96, respectively (p = 0.004 **). However, the positive control sample recorded log 4.19 at the end of storage.
When estimating the coliform group, all treatments of essential oils did not record any numbers, which shows the positive effect of all treatments compared to the control treatment which recorded log 4.1 at the end of the storage period (p = 0.007 **). These results might be due to the anti-microbial effect that characterizes essential oils because they contain active compounds such as carvacrol, thymol, limonene, and cinnamaldehyde [64][65][66].

Correlation Study
Pearson analysis in Table 4 shows a significant and positive correlation between total antioxidant content of treated strawberries and vitamin C, TPC, and CAT while a significantly negative relation was observed for weight loss and SSC content. The total antioxidant content of treated fruits significantly correlated negatively with decay percentage, respiration rate, and PPO. A similar relationship was noted for total microbial account and total coliform as well as mold and yeast. This correlation suggested that increasing the level of the antioxidant content in fruits plays an important role in reducing the changes in physicochemical properties and extending the shelf life of strawberries' fruits [4]. Conversely, the lack of a relationship was noted with fruit firmness, thus indicating that the fruit firmness could be correlated strongly with the water loss and fruit respiration, as mentioned by Badawy et al. [26].

Conclusions
Compared with untreated fruits, utilization of essential oils emulsion for orange, lemon and mandarin could effectively reduce the weight loss, respiration rate, human and plant pathogenic microorganisms, and activity of antioxidant enzymes (PPO and CAT) of strawberry fruits. In addition, our tested essential oil delayed the deterioration of fruit firmness, soluble solid content, and titratable acidity. Our treatments maintained ascorbic acid, anthocyanins and total phenols at the end of the storage period. The strawberry fruits had a higher antioxidant capacity at the end of storage, thus extending the storage ability and delaying fruit senescence. Therefore, emulsions of citrus essential oils had better preservation effects, and emulsion of mandarin essential oil was slightly better than orange and lemon essential oils.