Performance Evaluation of Two Commercially Available Portable Spectrometers to Non-Invasively Determine Table Grape and Peach Quality Attributes

: Near-infrared (NIR) spectroscopy has been used to non-destructively and rapidly evaluate the quality of fresh agricultural produce. In this study, two commercially available portable spectrometers (F-750: Felix Instruments, WA, USA; and SCiO: Consumer Physics, Tel Aviv, Israel) were evaluated in the wavelength range between 740 and 1070 nm to non-invasively predict quality attributes, including the dry matter (DM), and total soluble solids (TSS) content of three fresh table grape cultivars (‘Autumn Royal’, ‘Timpson’, and ‘Sweet Scarlet’) and one peach cultivar (‘Cassie’). Prediction models were developed using partial least-square regression (PLSR) to correlate the NIR absorbance spectra with the invasive quality measurements. In regard to grapes, the best DM prediction models yielded an R 2 of 0.83 and 0.81, a ratio of standard error of performance to standard deviation (RPD) of 2.35 and 2.29, and a root mean square error of prediction (RMSEP) of 1.40 and 1.44; and the best TSS prediction models generated an R 2 of 0.97 and 0.95, an RPD of 5.95 and 4.48, and an RMSEP of 0.53 and 0.70 for the F-750 and SCiO spectrometers, respectively. Overall, PLSR prediction models using both spectrometers were promising to predict table grape quality attributes. Regarding peach, the PLSR prediction models did not perform as well as in grapes, as DM prediction models resulted in an R 2 of 0.81 and 0.67, an RPD of 2.24 and 1.74, and an RMSEP of 1.28 and 1.66; and TSS resulted in an R 2 of 0.62 and 0.55, an RPD of 1.55 and 1.48, and an RMSEP of 1.19 and 1.25 for the F-750 and SCiO spectrometers, respectively. Overall, the F-750 spectrometer prediction models performed better than those generated by using the SCiO spectrometer data.


Introduction
Fruits and vegetables produced in California have a paramount impact on the national economy [1]. Among the top twenty agricultural commodities produced in this state, grapes (Vitis vinifera L.) are the number one fresh fruit with a total market value of more than 5.8 billion dollars (2017). California also leads the nation's production of many other commodities-meaning that it generates 99 percent or more-among which, peaches (Prunus persica L.) and grapes are again included [1]. Consequently, fruit producers and farm technicians are interested in devices capable of non-destructively measuring the internal quality of these fruits [2], and frequently ask for scientific evidence to local extension services. quality spectrometers of several brands, such as the F-750 (Felix Instruments, Camas, WA, USA), and SCiO (Consumer Physics, Tel Aviv, Israel). The latter belongs to the recent category of miniaturized spectrometers, with increasing interest nowadays due to their potential application inside mobile phones [25,26]; its capabilities have been applied to a wide range of studies, from cultivar detection on seeds [27] to the characterization of pharmacy tablets [23]. Therefore, potential users in particular agri-food producers, technicians, and extension personnel are interested in scientific studies and information regarding the prediction performance, and practical application of these equipment. These tools should be accurate, robust, and easy to use to allow real-time and in-field measurement conditions. For in-field non-destructive fruit quality attribute prediction, spectrometers should tolerate differences in fruit maturity, color, varieties, and environment. Thus, the goal of this study was to evaluate the potential of two commercially available hand-held portable non-invasive VIS/NIR produce quality spectrometers, including the Felix-750 and SCiO, to predict quality attributes (DM and TSS) in fresh table grapes and peach fruits.

Fruit Samples
Table grapes and peach fruits were selected among other species for this experiment due to two main reasons: First, their economic importance in local and national economy, and, second, their different size, which may affect light diffusion in their internal tissues and, therefore, final quality estimation. Samples were hand harvested from commercial grower fields on July 2017 in Bakersfield, in the case of table grapes, and in Esparto, CA, USA, in the case of peaches. Table grape samples included a total of 450 single berries from three cultivars containing different skin colors, 'Autumn Royal' (purple), 'Timpson Seedless' (green), and 'Sweet Scarlett' (red) (150 berries per cultivar); and 150 peach fruits from one cultivar ('Cassie'), as summarized in Table 1. Immediately after harvest, samples were transported in a cooler to the Postharvest Engineering laboratory at UC Davis and stored in a walk-in cold room (0 • C) before spectral measurements.

Fresh Fruit Spectral Measurements
Two commercially available hand-held produce quality spectrometers, as described in Table 2, were used in this study. The SCiO spectrometer was a brand new piece of equipment, acquired for the purpose of this experiment, while the F-750 spectrometer have been used before for other scientific projects at the Biological and Agricultural Engineering Dept. of University of California-Davis, having all the setup and calibration procedures adequately updated. In order to create temperature-compensated calibration models, reflectance spectra using the F-750 spectrometer were acquired twice on opposite sides on the surface of each sample (each berry in the case of grapes, and each peach fruit), at three different temperatures (0, 10, and 20 • C). Fruit samples were allowed to equilibrate in different cold rooms at the desired temperature for a minimum of 2 h, and spectra acquisition was performed inside each cold room. The same process was repeated using the SCiO spectrometer but, due to its short battery life, this was only performed at two different temperatures (0 and 20 • C). For each sample, a mean spectrum was calculated by averaging the total scan spectra (6 for the F-750 spectrometer, and 4 for the SCiO spectrometer) collected for each berry or fruit at the different temperatures.
In the case of the F-750 spectrometer, recorded spectral data for all of the fruit samples were transferred to a laptop computer (Intel Core i7-7700HQ CPU 2.80 GHz 16.0 GB RAM) using a Secure Digital (SD) card and the 'F-750 Model Builder' software Version 1.3.0.177 (Felix Instruments, Camas, WA, USA), and saved using a Comma-Separated Values (CSV) file format for later spectral processing. For the SCiO spectrometer, measured reflectance spectra were stored in the manufactures cloud service (Consumer Physics; Tel Aviv, Israel), using an iPhone 6s (Apple Inc. Cupertino, CA, USA), and saved in a laptop computer using a CSV file format for later spectral processing. Ultimately, reflectance spectra from the SCiO spectrometer were transformed to absorbance spectra, using Equation (1).
After spectral measurement and transformation were performed, data were imported into Matlab R2018a (version 9.4 Release March 2018, The Mathworks, Natick, Boston, MA, USA). Spectra data wavelength range selection, pre-processing, data analysis and visualization, and partial least-square (PLSR) regression calibration and prediction model development were performed in the Matlab environment and the PLSR Toolbox (version 7, Eigenvector Research, Inc. 2012, Manson, WA, USA).

Spectral Data Wavelength Range Selection, and Pre-Processing
Acquired spectra from a biological sample, such as a fresh fruit, typically contain high-and/or low-frequency interferences and irrelevant information, which might influence prediction performance, and accurate development of calibration/prediction models. Non-relevant information can be present due to intrinsic equipment electronic noise, dark current, shot and readout noise, sample background variations, unwanted incidence light (stray light) and scattering, changes in light intensity, and non-uniform distribution of light over the scanned surface. Defining the usable wavelength range for each spectrometer, and spectral pre-processing of the originally acquired spectra is imperative to remove any irrelevant information within the spectra, and to develop reliable and stable calibration and predictions models [3,28,29]. Therefore, due to differences in color between samples, the VIS range of the F-750 spectrometer was not included in the development of quality attribute prediction models. In addition, the usable wavelength range for both spectrometers (740-1070 nm) was further defined by evaluating their performance (SNR, and signal variation with room temperature) with 25.3 mm diameter Polytetrafluoroethylene (Teflon) spheres as reference standards/phantoms on a separate experiment. These spheres are chemically inert, moisture and high-heat resistant. Spectra of the reference sphere were measured on top of the Teflon sphere at three different room temperatures Agronomy 2020, 10, 148 5 of 16 (0, 10, and 20 • C). For each sphere, the spectrum was measured 5 times in sequence, and a total of 25 spectrums (3 repetitions of 5 measurements) were recorded per temperature. A total of 75 spectrums were analyzed per spectrophotometer in the spectral range 740-1070 nm. The average coefficient of variation (CV) of the measured spectrums wavelength by wavelength for each temperature was calculated, as well as the SNR.
Also, with the aim of improving the calibration models, several well-known mathematical pre-processing techniques were applied to the originally acquired spectra (original), including the Standard Normal Variate (SNV), Orthogonal Signal Correction (OSC), Multiplicative Scattering Correction (MSC), First Derivative (FD), and Second Derivative (SD), and smoothing using the Savitzky-Golay (SG) algorithm [7,13].

Fruit Quality Attribute Measurements (DM, and TSS)
After spectral measurements, each sample fruit was immediately processed to estimate its DM (%) and TSS ( • Brix) content, as reference measurements (quality attributes). The initial weight of the approximate one-quarter of each fruit was recorded and then dehydrated until constant mass was reached in an oven at 110 • C temperature. The DM content of each sample was then calculated by dividing the dry weight by its corresponding initial (wet) weight.
The second half of each peach sample fruit was juiced using a juice extractor (Big Mouth Pro Juice Extractor, Hamilton Beach, Glen Allen, VA, USA) and thoroughly mixed. The second half of each table grape sample fruits were manually squeezed by firmly pressing each fruit, and thoroughly mixed. Then, to measure the TSS content, a juice aliquot containing 0.3 mL of juice was placed onto a digital hand-held refractometer (Atago PAL-1, Tokyo 105-0011, Japan) with an accuracy of ±0.2%, as specified by the manufacturer. DM and TSS reference measurements were performed in duplicate, while fruits were held at a 20 • C constant temperature.

Modeling the Relationship between Spectral Data and Quality Attributes
Partial Least-squares Regression (PLSR) was employed to model the relationship between the spectral and invasive quality attribute data (DM, and TSS) of both fruits (table grapes, and peach). PLSR essentially predict each quality attribute vector (Y) from the spectral matrix (X) by modeling the shared structure between the two, and extracting a group of orthogonal (or statistically independent) latent variables (LV), while simultaneously decomposing X and Y [11]. This translates into finding components along directions of maximal covariance between X and Y. For the regression then the focus is concentrated on these orthogonal factors to model the relationship [7].
Data were categorized into spectra and quality attributes matrices per the PLSR algorithm and randomly divided into a 75% for the calibration set, and 25% for the prediction. Cross-Validation (CV) divided the calibration set into 10-folds with 20 iterations using the Venetian blinds method. When predicting quality attribute values for the prediction set, the PLSR algorithm calculates new LV estimates as linear combinations of the original variables from the spectra matrix and uses these estimates as predictors. Minimum value for the root-mean square error of cross-validation (RMSECV) is used to select the optimum number of latent variables (LV) (max was fixed as 20) used to internally calculate the best model for each quality attribute [30][31][32]. RMSECV is obtained by comparing the predicted trait value with its reference value. In addition to the number of LV, PLSR statistical parameters to estimate performance of the selected model included R 2 cal and R 2 pred (the coefficient of determination for calibration and prediction, respectively), SEC and SEP (standard error of calibration and prediction, respectively), RPD (residual predictive deviation), and Bias (average difference between predicted and actual values). PLSR models with maximum R 2 and RPD, and lowest SEC, SEP and Bias values were selected as the optimum pre-processing method and best regression calibration models [3,[33][34][35][36].

Results and Discussion
Depicted in Figure 1 are the original spectra of the samples, averaged per cultivar (green, purple and red grapes, and peaches), acquired with the SCiO spectrometer. Even though this device records data in the NIR region between 740 and 1070 nm, larger differences were observed between species than between varieties, having peach spectra a broader light absorption range than the grape spectrum. This behavior was also corroborated by NIR+VIS spectra acquired with the Felix device (not shown), where differences also in the VIS region were larger between fruit species due to their respective skin and flesh colors. Consequently, data analyses were done from this point on combining the spectra of all table grapes, with the aim of obtaining robust global models for each fruit species, and not only for single cultivars.
Agronomy 2020, 10, x FOR PEER REVIEW 6 of 17 calibration and prediction, respectively), RPD (residual predictive deviation), and Bias (average difference between predicted and actual values). PLSR models with maximum R 2 and RPD, and lowest SEC, SEP and Bias values were selected as the optimum pre-processing method and best regression calibration models [3,[33][34][35][36].

Results and Discussion
Depicted in Figure 1 are the original spectra of the samples, averaged per cultivar (green, purple and red grapes, and peaches), acquired with the SCiO spectrometer. Even though this device records data in the NIR region between 740 and 1070 nm, larger differences were observed between species than between varieties, having peach spectra a broader light absorption range than the grape spectrum. This behavior was also corroborated by NIR+VIS spectra acquired with the Felix device (not shown), where differences also in the VIS region were larger between fruit species due to their respective skin and flesh colors. Consequently, data analyses were done from this point on combining the spectra of all table grapes, with the aim of obtaining robust global models for each fruit species, and not only for single cultivars.  Figure 2 shows the mean of absorbance spectra of 450 table grapes and 150 peach fruits acquired by the F-750 and SCiO spectrometers at NIR region 740-1070 nm, respectively. Although the absorbance intensity varied, all the spectra had similarities in their overall pattern and shape. It is seen that the spectra showed one broad absorbance peak around 975 nm, which can be associated with water and sugar absorption [37,38]. The raw spectral data also contain undesirable interferences such as overlapped bands, scattering and random noises, and therefore these spectra were subjected to pre-processing methods. The spectra that generated the combination of the minimum RMSE, higher R 2 and RPD, bias near zero, and optimized numbers of LV were considered for the PLSR modelling. As mentioned, for model development and performance evaluation for predicting DM and TSS for table grapes and peach, only the NIR range from 740 to 1070 nm was considered for both spectrometers, discarding the VIS wavelengths of F-750.  Figure 2 shows the mean of absorbance spectra of 450 table grapes and 150 peach fruits acquired by the F-750 and SCiO spectrometers at NIR region 740-1070 nm, respectively. Although the absorbance intensity varied, all the spectra had similarities in their overall pattern and shape. It is seen that the spectra showed one broad absorbance peak around 975 nm, which can be associated with water and sugar absorption [37,38]. The raw spectral data also contain undesirable interferences such as overlapped bands, scattering and random noises, and therefore these spectra were subjected to pre-processing methods. The spectra that generated the combination of the minimum RMSE, higher R 2 and RPD, bias near zero, and optimized numbers of LV were considered for the PLSR modelling. As mentioned, for model development and performance evaluation for predicting DM and TSS for table grapes and peach, only the NIR range from 740 to 1070 nm was considered for both spectrometers, discarding the VIS wavelengths of F-750.

PLSR Models for Predicting the DM and TSS of Table Grape and Peach Fruits
Calibration and prediction models based on PLSR to infer the DM and TSS of table grapes and peach fruits were developed in the range of 740-1070 nm region using the F-750 and SCiO spectrometers. The results obtained for the original and pre-treatment spectral data of both spectrometers in calibration and prediction models are shown in Table 3 for table grapes and peach. Regarding the different pre-processing techniques applied, the PLSR calibration model using OSC performed better for predicting table grape DM using the F-750 and SCiO spectrometers, which had the highest R 2 (0.79 and 0.75), lowest RMSEC (1.55 and 1.66), and highest RPD (2.17 and 2.02), and contained optimal number of LV (nine and 11). Similarly, the best PLSR calibration models were obtained for SNV spectra for predicting TSS in table grapes using both spectrometers, showing a very high value of R 2 (0.99 with F-750, and 0.97 with SCiO), RPD (8.96 and 5.87) and a fairly low value of RMSEC (0.36 and 0.55) with a larger number of LV (19 and 14) than for DM. The overall calibration results obtained in the original and pre-processing spectra were not noticeable predictors for DM and TSS in peach, but slightly better results were obtained in prediction (e.g., larger value of R 2 and RPD, and smaller value of RMSEP) using pre-processing techniques as shown in Table 4. The obtained results using SD pre-processing with eight LV and FD pre-processing with six LVs performed better for the F-750 spectrometer, and SG with nine LV and SNV with eight LV performed better for the SCiO spectrometer for the prediction of peach DM and TSS, respectively.

PLSR Models for Predicting the DM and TSS of Table Grape and Peach Fruits
Calibration and prediction models based on PLSR to infer the DM and TSS of table grapes and peach fruits were developed in the range of 740-1070 nm region using the F-750 and SCiO spectrometers. The results obtained for the original and pre-treatment spectral data of both spectrometers in calibration and prediction models are shown in Table 3 for table grapes and peach. Regarding the different pre-processing techniques applied, the PLSR calibration model using OSC performed better for predicting table grape DM using the F-750 and SCiO spectrometers, which had the highest R 2 (0.79 and 0.75), lowest RMSEC (1.55 and 1.66), and highest RPD (2.17 and 2.02), and contained optimal number of LV (nine and 11). Similarly, the best PLSR calibration models were obtained for SNV spectra for predicting TSS in table grapes using both spectrometers, showing a very high value of R 2 (0.99 with F-750, and 0.97 with SCiO), RPD (8.96 and 5.87) and a fairly low value of RMSEC (0.36 and 0.55) with a larger number of LV (19 and 14) than for DM. The overall calibration results obtained in the original and pre-processing spectra were not noticeable predictors for DM and TSS in peach, but slightly better results were obtained in prediction (e.g., larger value of R 2 and RPD, and smaller value of RMSEP) using pre-processing techniques as shown in Table 4. The obtained results using SD pre-processing with eight LV and FD pre-processing with six LVs performed better for the F-750 spectrometer, and SG with nine LV and SNV with eight LV performed better for the SCiO spectrometer for the prediction of peach DM and TSS, respectively. The calculated RMSECV values for PLSR is plotted as a function of the number of LVs in Figure 3. Overall, the number of LVs considered for model development were in the acceptable range for all of the models, because the number of samples within the calibration data set were ten times larger than the LVs [39]. However, the minimum RMSECV was observed in the 19th and 14th LVs for the prediction of TSS in table grapes using the F-750 and SCiO spectrometers, respectively. The latter indicates that a large number of factors were interpreted to construct the models. From Figure 3, it is seen that the first minimum RMSECV value was observed in the 7th LV (marked by blue dotted circle) to predict the TSS using the F-750, capturing 100% of the variance by the regressor and explaining 97.16% of its variance ( Table 3). The difference of the RMSECV between the 7th and 8th LVs and thereafter were relatively low and insignificant, indicating that LVs above seven might contain irrelevant information and will cause an over fitted model. Hence, 7 were the optimal number of LVs chosen to predict table grape TSS using the F-750 spectrometer. Similarly, to avoid irrelevant information and model over fitting, the optimal number of LVs (marked by blue dot circle) to predict the TSS in table grapes using the SCiO spectrometer was equal to 9.    Table Grapes and Peach Figure 4A-D show the results for the prediction of table grape DM in PLSR regression using the best calibration model developed by OSC spectra using the F-750 and SCiO spectrometers, respectively. These figures demonstrated that good results were obtained for table grape DM prediction using both produce quality spectrometers. These results are aligned with the ones obtained by [24] using also the F-750 and SCiO spectrometers for DM assessment on apples and kiwis, with R 2 values between 0.8 and 0.95. In the case of the F-750 spectrometer, the coefficient of determination, RMSEP, and RPD for the DM prediction set varied from 0.52 to 0.83, 1.40 to 4.11, and 0.80 to 2.35, respectively ( Table 3). As seen in Table 4 the application of pre-processing techniques on the PLSR model had less effect on prediction performance but the calibration model using OSC was slightly improved the model's predictive power with R 2 = 0.83, RMSEP = 1.40, RPD = 2.35, and bias = −0.27 ( Figure 4B). On the other-hand, R 2 , RMSEP, and RPD for the DM prediction set using the SCiO spectrometer varied between 0.58-0.81, 1.45-2.66, and 1.46-2.29, respectively ( Table 3). The PLSR model with OSC and smoothing spectra showed the same coefficient of determination (R 2 = 0.81) but considering other statistical parameters, the PLSR model with OSC ( Figure 4D) was chosen as the best model for the prediction of DA in table grapes using the SCiO spectrometer. There is no previous study that addressed the direct measurement of DM in fresh table grapes using NIR spectroscopy. However, ref. [40] have reported the measurement of DM in homogenized table grape samples using the NIR region from 1100 to 2500 nm. They found better prediction results (R 2 = 0.90, SEP = 1.34, RPD = 2.2, and bias = 0.48) in their NIR-based PLSR model. Figure 4E-H show the results of PLSR models developed for the prediction of peach DM using the F-750 and SCiO spectrometers, respectively. These figures and Table 4 revealed that for both spectrometers, improved results were obtained in the prediction set than in calibration after applying pre-processing. The R 2 and RPD were achieved at 0.81 and 2.24 for the F-750 spectrometer using the best PLSR model in SD spectra and eight LVs ( Figure 4F) and 0.67 and 1.74 for the SCiO spectrometer using the best PLSR model in SG spectra and nine LV ( Figure 4H). One study was found that measured the DM content in peach using NIR technique but obtained unsatisfactory results [19]. Considering the model chemometric indicators, overall, the F-750 spectrometer performed comparatively better than the SCiO spectrometer for measuring the DM in peach fruits; this can be associated with a lower variability of measurements, as explained later in Section 3.4.

Model Prediction Performance to Infer TSS Content in Table Grapes and Peach
The models developed with SNV spectra to predict the TSS in table grapes using the F-750 and SCiO spectrometers achieved an excellent value for the coefficient of determination for both spectrometers across the range of 4.35-21.1 • Brix. Figure 5A-D shows the results of correlation between the measured and predicted values of table grape TSS in calibration and prediction using the PLSR model and SNV pre-processing. The application of SNV pre-processing technique on the PLSR model significantly improved the results of both calibration and prediction. In particular, the value of RPD increased solidly in comparison with original spectra. In prediction, the R 2 , RMSEP, RPD, and bias was obtained at 0.97, 0.53, 5.95, and 0.01, respectively, using seven LV for the F-750 spectrometer, and was obtained at 0.95, 0.70, 4.48, and 0.08, respectively, using nine LV for the SCiO spectrometer, evidently showing excellent model performance [41]. These results also demonstrated that the developed model for direct measurement of TSS in fresh grapes produced better prediction results when compared with the results obtained by [42] (R 2 = 0.95, RMSEP = 1.011), [15] (R 2 = 0.91, RMSEP = 1.42, RPD = 3.36), [43] (R 2 = 0.94, SECV = 1.0, RPD = 4.12), and [18] (R 2 = 0.82, RMSEP = 1.48). RPD is one of the important model performance evaluation chemometric indexes and the higher RPD confirmed the model prediction accuracy and robustness [9]. Considering the value of RPD, it is also seen that the F-750 spectrometer performed best for measuring TSS in table grape fruits than the SCiO spectrometer. Figure 5E-H shows the results of the PLSR models of TSS prediction in peach using the F-750 and SCIO spectrometers, respectively. The R 2 , RMSEP, RPD, and bias of the external validation set were 0.62, 1.19, 1.55, and 0.31, respectively, for F-750 and 0.55, 1.25, 1.48, and 0.15, respectively, for the SCiO spectrometer (Table 4). These model evaluation indexes indicated that the PLSR prediction model for TSS in fresh peach were not satisfactory for either of the spectrometers and had poor predictive performance in comparison to table grapes. Two reasons may explain this result: First, the number of samples (berries) in the grape models was three times higher than in the case of peaches; second, the maturity state of some of the peaches was quite high (too ripe), resulting in a narrower range of TSS (4.35-21.1 Brix for grapes, and 21.1-17.85 Brix for peaches, Table 1).
Agronomy 2020, 10, x FOR PEER REVIEW 11 of 17  With the aim of summarizing these results, it can be stated that the chemometric indexes for DM and TSS models showed acceptable results and demonstrated that the NIR spectra region (740-1100 nm) coupled with PLSR regression could effectively predict the DM and TSS across the range of 6.12-24.61% and 4.35-21.1 • Brix, respectively, in table grapes using the F-750 and SCiO spectrometers. Both quality spectrometers therefore could be used to non-destructively determine DM and TSS quality attributes in fresh table grapes. The PLSR results of fresh peach DM and TSS were poorly predicted by both spectrometers. Based on the chemometric indicators of the DM prediction set, the F-750 spectrometer produced relatively better results than SCiO with R 2 = 0.81, RMSEP = 1.28, and RPD = 2.24 and could potentially be used for determination of DM in peach using PLSR regression. Overall, it can be inferred that the F-750 had a higher performance in comparison to the SCiO for the prediction of DM and TSS; again, this can be due to more stable spectral readings, according to results of Section 3.4.

Performance Evaluation Using Reference Spheres
From the overall comparison of the models obtained using the two produce quality meters, it is suggested that the F-750 DM and TSS prediction performance is higher in comparison to the SCiO and can be suitable to non-destructively measure fruit quality attributes. However, the performance of both meters was further investigated using a solid object (inert reference) to confirm the PLS results.
The spectra acquisition with biological samples is often complicated and contains high-and/or low-frequency interferences and irrelevant information due to non-uniform distribution of light over the surface [29]. Hence, a wavelength by wavelength comparison was made between the spectrum of F-750 and SCiO meters obtained using a standard solid object (white Teflon sphere). The average coefficient of variation (CV) of the measured spectrums for each temperature is shown in Table 5. It is seen that the SCiO spectrometer yielded a higher variation per wavelength within the spectrum range acquired at each temperature, and as well as between the spectrum acquired at different room temperatures with an overall mean variation equal to 18.39%. The percent of variation per wavelength within and between the spectrums observed was almost zero for F-750, indicating that the acquired spectra using this meter apparently showed a similar pattern for all three different room conditions, and the lowest variability between the spectrometers. When inferring the SNR of the acquired absorbance Teflon spectra with both spectrometers (Figure 6), strong differences between the devices can be observed. Highly noisy signals can be observed near the limits of their wavelength ranges, and even though both meters react to sample temperature variations at different levels, both show stable signals in the NIR region used to build prediction PLSR models in this study (740 to 1070 nm). However, the SNR in the case of the F-750 is one hundred times higher than for the SCiO, potentially affecting the performance of the PLRS prediction models. The higher noise in the F-750 spectrometer did not seem to affect its overall prediction performance as it is still better than the SCiO performance. This variation can be potentially due to the intrinsic design of each of the spectrometers, including the incident light intensity and type, and additional electrical components.

Performance Evaluation Using Reference Spheres
From the overall comparison of the models obtained using the two produce quality meters, it is suggested that the F-750 DM and TSS prediction performance is higher in comparison to the SCiO and can be suitable to non-destructively measure fruit quality attributes. However, the performance of both meters was further investigated using a solid object (inert reference) to confirm the PLS results.
The spectra acquisition with biological samples is often complicated and contains high-and/or low-frequency interferences and irrelevant information due to non-uniform distribution of light over the surface [29]. Hence, a wavelength by wavelength comparison was made between the spectrum of F-750 and SCiO meters obtained using a standard solid object (white Teflon sphere). The average coefficient of variation (CV) of the measured spectrums for each temperature is shown in Table 5. It is seen that the SCiO spectrometer yielded a higher variation per wavelength within the spectrum range acquired at each temperature, and as well as between the spectrum acquired at different room temperatures with an overall mean variation equal to 18.39%. The percent of variation per wavelength within and between the spectrums observed was almost zero for F-750, indicating that the acquired spectra using this meter apparently showed a similar pattern for all three different room conditions, and the lowest variability between the spectrometers. When inferring the SNR of the acquired absorbance Teflon spectra with both spectrometers (Figure 6), strong differences between the devices can be observed. Highly noisy signals can be observed near the limits of their wavelength ranges, and even though both meters react to sample temperature variations at different levels, both show stable signals in the NIR region used to build prediction PLSR models in this study (740 to 1070 nm). However, the SNR in the case of the F-750 is one hundred times higher than for the SCiO, potentially affecting the performance of the PLRS prediction models. The higher noise in the F-750 spectrometer did not seem to affect its overall prediction performance as it is still better than the SCiO performance. This variation can be potentially due to the intrinsic design of each of the spectrometers, including the incident light intensity and type, and additional electrical components.
(A) (B) Figure 6. Mean signal-to-noise ratio (SNR) of Teflon absorbance spectra measured at different room temperatures with the F-750 meter (A) and with the SCiO meter (B), across their respective full wavelength range.

Conclusions
Two NIR produce quality spectrometers were investigated in this study to evaluate their performance to non-invasively determine quality attributes for three table grape cultivars ('Autumn Royal', 'Timpson', and 'Sweet Scarlet') and one peach cultivar ('Cassie') using PLSR models in the 740-1070 nm wavelength range and various pre-processing techniques. Results indicated that both spectrometers performed well in predicting DM (R 2 > 80%) and TSS (R 2 > 90%) contents in fresh table grapes. Regarding peach, the F-750 spectrometer performed comparatively better than the SCiO spectrometer and yielded high prediction accuracy DM (R 2 = 0.81 and RPD = 2.24). Overall, both spectrometers were not effective in predicting the TSS of fresh peach. Among the two spectrometers, the F-750 spectrometer seems suitable for practical use and could readily be used for field applications for predicting the quality attributes in fresh table grapes and peach fruits. To improve the modeling performance and to confirm the reliability of both spectrometers to predict quality attributes of fresh peaches, especially the content of TSS, further studies are recommended.