Article The Influence of Synthesis Parameters on Particle Size and Photoluminescence Characteristics of Ligand Capped Tb 3+:LaF3

Organic ligand surface-treated Tb3+:LaF3 was synthesized in water and methanol for subsequent incorporation into polymethyl methacrylate (PMMA) via solution-precipitation chemistry in order to produce optically active polymer nanocomposites. Nanoparticle agglomerate diameters ranged from 388 ± 188 nm when synthesized in water and 37 ± 2 nm when synthesized in methanol. Suspension stability is paramount for producing optically transparent materials. Methanol nanoparticle synthesized at a pH of 3 exhibited the smallest agglomerate size. Optical spectroscopy, dynamic light scattering, transmission electron microscopy, scanning transmission electron microscopy, and zeta potential analysis were used to characterize the particles synthesized.


Optically-Active Polymer Nanocomposites
Optically-active polymer nanocomposites are suitable materials for use in a series of optoelectronic applications due to their low cost and ease of processing [1,2].Amorphous polymers such as polymethyl methacrylate (PMMA) are utilized in numerous optical applications because they are highly transparent in the visible.PMMA has been the most predominate polymer employed to date for optical purposes and has been shown to be a suitable medium for certain rare-earth (RE) ion ligand complexes [3] that generate light.While the higher attenuation of polymers, relative to inorganic glasses (e.g., silica optical fiber), limits their use to distances of less than about 1 km [4], there are still many applications for light emissive polymer fibers, films, and coatings.
Luminescent species such as RE ions have been utilized as active additives to produce emissions in the visible region of the electromagnetic spectrum for various inorganic glasses and crystals.RE ions can produce intense narrow spectral emissions with long excited-state lifetimes, in the range of milliseconds, as a result of their partially-filled and electrostatically-shielded 4f electronic levels [5,6].These advantageous radiative properties of rare-earth ions unfortunately tend to be quenched by the inherently high vibrational energies of polymers [7].Additionally, RE ion salts exhibit limited solubility in polymers and these salts tend to aggregate resulting in enhanced scattering as well as luminescent concentration quenching at low concentrations [8].
Polymer nanocomposites comprised of organic ligand capped inorganic nanocrystals are studied here in order to merge the spectroscopic benefits of RE ions in low vibrational energy inorganic hosts with the processability of polymeric materials.More specifically, the function of a surface organic ligand (acetylsalicylic acid; ASA) is used to absorb and transfer UV light to RE ions that are doped into inorganic nanocrystals dispersed into PMMA.ASA is an aromatic carboxylic acid and aromatic acids have been used as ligands to coordinate, to sensitize and to enhance lanthanide fluorescence of RE ions [9][10][11].The inorganic nanocrystal (lanthanum trifluoride; LaF 3 ) provides a low phonon energy environment (350 cm −1 ) and offers channels for charge transfer excitations from near anionic ligands [12,13].LaF 3 also exhibits a high solubility for RE ions dopants of the same valence within the crystal [14].In this work, the RE ion of choice is trivalent terbium (Tb 3+ ).The most intense emission band of Tb 3+ from direct excitation at 350 nm is located at about 543 nm which emits green colored light that is easily distinguished visually.
Energy transfer from the ligand to the RE-doped nanocrystal begins with the excitation of the ligand, as is illustrated in Figure 1.Organic ligands typically absorb energy in the near ultraviolet (UV) spectral region ranging from 200 nm to about 400 nm [15].Typically, singlet spin states of the ligand are excited followed by intersystem crossing from the singlet state to the lowest triplet state within the ligand through thermal relaxation accompanied by the generation of heat.The occurrence of intersystem crossing is in competition with phonon deactivation of the excited singlet state and, potentially, fluorescence from the ligand [8].Energy transfer may then occur from the triplet state of the ligand to the RE ion when the energy of the triplet state is reasonably equivalent to that of a suitable energy level of the dopant.Light emission from the RE ion results from the radiative transition between 4f states.

Review of Electrical Double Layer Theory
Nanoparticles, such as those employed here, generally refer to materials whose dimensions are within the range of 1-100 nm.At these small sizes, surface interactions strongly influence their properties [17].The electrical double layer is a representation of the ion distribution at the interface between a solid and a solvent in solution/suspension as is illustrated in Figure 2. Helmholtz first introduced and termed the idea of the electrical double layer which was later extended by Gouy-Chapman and Stern [18][19][20][21][22].The Stern layer is the first stratum that consists of a dense layer of ions of the opposite charge (counter ions) that form around the nanoparticle.The second layer is the diffuse layer which is the charged atmosphere of ions of the opposite charge to the nanoparticle that extends away from the Stern layer.The high concentration of counter ions within the diffuse layer gradually decreases with increasing distance from the nanoparticle until equilibrium is reached with the ion concentration in the bulk of the solvent [23].The width of the electrical double layer is characterized by the thickness of the diffuse layer or Debye screening thickness which is represented by κ −1 (Equation 1).The relative thickness of the diffuse layer coincides with the ability of the nanoparticle to repel (colloidally stable) or attract other nanoparticles (agglomeration).Agglomerates form as the electrical double layer is condensed by the nanoparticles attempting to reduce its surface area to minimize interfacial energy [24].The scattering of light will occur as agglomerate sizes increase; consequentially, diminishing the transparency of the polymer nanocomposites; which influences the ability of the RE ion to absorb and emit efficiently.Therefore, it is important that a polymer with additives be optically transparent in the region of the excitation and emission wavelengths of the RE ion [13].
(1) The major contributors to the thickness of the diffuse layer are ionic strength of the electrolyte (I), absolute temperature in Kelvin (T) and dielectric constant of the solvent (ε r ).The ionic strength is inversely proportional to κ −1 .Therefore, as the ionic strength lowers the double layer thickness should increase potentially resulting in less agglomeration; hence, low optical scattering.The Debye screening thickness is directly related to the dielectric constant and temperature.Temperature and the dielectric constant of the solvent are indirectly related to each other which may lead to only a slight change in the Debye screening thickness.

Methods of Nanoparticle Synthesis Review
Reverse micelle synthesis, sol-gel chemistry, solvothermal, hydrothermal and aqueous solution-polymer precipitation have all been utilized to produce inorganic nanoparticles [17,[25][26][27][28]. Reverse micelle synthesis require significant amounts of surfactants to produce limited amounts of nanoparticles [28].Solvothermal and hydrothermal techniques require an autoclave at elevated temperatures and pressures.Sol-gel chemistry and solution-polymer precipitation require a drying step which may increase aggregation.In the present work, aqueous solution-polymer precipitation techniques were chosen as the result of the possibility to control size and size distribution of the nanoparticle [29].We report on the effect of solvent relative dielectric constant, synthesis pH, and temperature on the diameter of ASA:Tb 3+ :LaF 3 nanoparticles in solution and PMMA via solution-polymer precipitation.

Results and Discussion
Elemental composition of the nanoparticles determined by EDX analysis is summarized in Table 1.The resulting composition consisted of Tb 3+ :LaF 3 at a 5:1 and 2:1 (La:Tb; i.e., (Tb 0.16 La 0.84 )F 3 and (Tb 0.33 La 0.67 )F 3 ) molar ratio for the nanoparticles synthesized in water and methanol solvents respectively.The main absorption peak for the ASA ligand is located at about 276 nm when the ligand is dispersed in water and methanol.A representative absorption spectra of the ASA ligand (blue), Tb(NO 3 ) 3 • 6H 2 O, (green), and Tb 3+ :LaF 3 (red) is shown in Figure 3.This excitation wavelength was used to evaluate the energy transfer of the ligand to the RE ion.The peak value for Tb(NO 3 ) 3 • 6H 2 O and Tb 3+ :LaF 3 respectively was measured at 340 nm.Excitation spectra of Tb 3+ and ASA at the 540 nm emission wavelength were measured and are displayed in Figure 4. Direct excitation of Tb 3+ (350 nm) and ligand excitation (276 nm) were evaluated for each solvent as is shown in Figure 5(a,b), respectively.
The emissions spectra for the nanoparticle loaded polymer (PMMA:ASA:Tb 3+ :LaF 3 ) upon direct ion and ligand excitation exhibited characteristic Tb 3+ emissions at 490, 543, 585, and 621 nm corresponding to the 5 D 4 → 7 F 6 , 5 D 4 → 7 F 5 , 5 D 4 → 7 F 4 , and 5 D 4 → 7 F 3 transitions, respectively.Energy transfer from the ligand to the RE ion is presumed by the excitation of the ligand and resulting characteristic emissions from the Tb 3+ .Organic dyes utilized in photonic devices produce broad emission spectra (FWHM = 50-200 nm) where narrow emission spectra (FWHM < 5 nm) are generally preferred [30] for improving color saturation [31] in various light-emission applications such as displays and fashion.The photoluminescence of the polymer nanocomposites synthesized in water and in methanol was measured by normalizing the spectra at 543 nm and applying Lorentzian fits.The FWHM at the 490 nm emission peak was computed as it provides insights into the affects of changes in the environment resulting from the ligand and agglomeration [32].In both cases of direct ion and ligand excitation, the spectral peaks are reasonably narrow (~9 nm for water and ~8 nm for methanol), though not statistically different given the spectrometer slit-width.
The interaction between temperature and dielectric constant in relationship to the thickness of the electric double layer is key factor in agglomerate size.Water has a relative dielectric constant of ~80 at 25 °C and ~64 at 70 °C and methanol has a dielectric constant of ~33 at 25 °C [33,34].The lowering of the dielectric constant of water with the synthesis temperature being elevated to 70 °C still provides water with a relative dielectric constant that is twice that of methanol at 25 °C.The Debye screening thickness of the nanoparticles synthesized in water should enable the particles to repel one another better than the nanoparticles synthesized in methanol based on the water synthesis maintaining a higher dielectric constant and increased fabrication temperature.Also, the nanoparticles synthesized in methanol should have effectively decreased the electrical double layer between the particles resulting in electrostatic attraction and greater agglomeration.Nanoparticle synthesis performed by Ellerbrock using water as the solvent found that Tb 3+ :LaF 3 particles without ligand measured 6 ± 1 nm in diameter [16].The average size of the agglomerate diameters as determined by dynamic light scattering for particles synthesized in water and methanol are listed in Table 2. Nanoparticle synthesized in methanol produced agglomerates that were, on average, an order of magnitude smaller than the agglomerates produced in water, which is contrary to the above statements and is illustrated in Figure 6.The reduction in size of the agglomerates fabricated in methanol could be attributed to methanol having fewer number of hydrogen bonds per molecule where a stronger ionic interaction between the nanocrystal and the ligand would be induced [35,36].Another factor effecting ionic interaction is pH as charges on the surface groups can be varied by changing the pH of the synthesis.Nanoparticles synthesized in water were fabricated at a pH of 8 where as the pH of the methanol reaction was not adjusted and was processed at an acidic pH of 2-3.The methanol synthesis was chosen for further evaluation.
The pH of the methanol nanoparticle suspension was increased by 1 pH unit starting with a pH of 3 to a pH of 8 in Figure 7(a,b), respectively.Figure 7  Particles displayed positive surface charges in this research as determined by zeta potential measurements, which represents the degree of repulsion between adjacent particles having the same charge [37].The positive surface charge can be counteracted by the addition of a Lewis base into the suspension which can promote the strengthening of negative charges leading to colloidal instability.Suspensions with zeta potential values between +30 to −30 mV are considered to be unstable.Zeta potential values below the black dotted line in Figure 8 indicate values below +30 mV; i.e., unstable suspensions.The zeta potential measurements verify the visual analysis of potential agglomerate formation beyond the synthesis pH of 3. Average agglomerate diameters as determined by dynamic light scattering for the particles synthesized at the various pH are listed in Table 3. TEM image illustrated in Figure 9 validate the diameter size difference between nanoparticles synthesis at a pH of 3 as compared to a pH of 8.

Nanoparticle Synthesis
Tb 3+ :LaF 3 nanocrystals were prepared in aqueous solutions with water as the solvent for the water process and methanol as the solvent for the methanol process.The water and methanol synthesis processes are depicted in Figure 10(a,b) respectively.The rare-earth solution of La(NO 3 ) 3 • 6H 2 O (9 mmol) and Tb(NO 3 ) 3 • 6H 2 O (2 mmol) in 16 mL of solvent, water and methanol respectively, was prepared at room temperature.NH 4 OH was added to the rare-earth solution in water to adjust the solution to pH 8.The pH of the methanol solution was not adjusted.A centrifuge was used to separate the precipitate at a setting of 3,000 rpm for 10 min.The nanoparticles produced from the water suspension were washed with 50 vol % of EtOH in water followed by an acetone wash.Synthesized particles were dried over night in a vacuum oven and then added to 5 wt % of PMMA in THF to form the polymer nanoparticle suspension, ASA:Tb 3+ :LaF 3 :PMMA/THF.ASA:Tb 3+ :LaF 3 :PMMA/THF was added drop wise to excess (v/v of suspension to MeOH; 1:400) stirred MeOH at ~3 °C.The product was precipitate nanocomposite polymer powder.
Methanol Synthesis: The rare-earth solution in methanol was added drop-wise into a stirred solution of NH 4 F (12 mmol) and ASA (23 mmol) in methanol at room temperature, ~25 °C.The volume ratio of methanol/ASA was 40:1.The pH was not adjusted.The suspension was stirred for 1 h.A centrifuge was used to separate the precipitate at a setting of 3,000 rpm for 10 min.Methanol synthesized particles were washed by a two-cycle neat methanol wash.Neat MeOH was added to the suspension and the temperature of the stirred suspension was decreased to approximately 3 °C.After 30 min, a solution of 5 wt % of PMMA in THF was added drop wise to the ASA:Tb 3+ :LaF 3 :MeOH suspension at a v/v of 1:400.The product was precipitate nanocomposite polymer powder.
The precipitate nanocomposite polymer powder was vacuum filtered, washed in MeOH and then dried under vacuum for over 12 h.

Characterization
The composition of the nanoparticles were characterized utilizing a scanning transmission electron microscope (STEM, Hitachi HD2000, Pleasanton, CA, USA) equipped with an Oxford INCA Energy 200 Energy Dispersive Spectrometer (EDX).
Optical absorption was performed using a Perkin Elmer Lambda 900 UV-Vis-NIR Spectrometer (Waltham, MA) with UV Winlab Version 3.00.03software.A 1 nm slit size in the UV and visible range of 260-380 nm was used to perform the scans.Samples were prepared by dispersing the ligand into water and methanol where all measurements were done at room temperature.
A Jobin-Yvon Fluorolog Tau 3 Fluorometer (Edison, NJ, USA) with 4 nm emission bandpass was used for emission spectra measurements and 1 nm slit width was used for excitation spectra measurements.1 nm intervals with 50 ms integration time was used to collect the data.Measurements were performed at room temperature.Lorentzian curve fits correcting for a constant background in Igor Pro 6.1 (Wavemetrics, Portland, OR, USA) were superimposed on excitation spectra for full-width at half maximum (FWHM) measurements.
Zetasizer Nano Series Nano ZS-Dynamic Light Scattering (DLS) module from Malvern (Worcestershire, UK) was utilized to measure the size and size distribution of particles at room temperature.Nanoparticles fabricated in water were ball milled using Teflon ® spheres into a fine powder before resuspension in neat water.The liquid suspension of nanoparticles in methanol was also measured.The average of three samples was used to determine the size distributions.
Electron micrographs were obtained using a STEM and a transmission electron microscope (TEM, Hitachi H7600T, Pleasanton, CA, USA).Samples were prepared by drop casting nanoparticle suspensions followed by subsequent solvent evaporation onto a 200 mesh carbon coated copper TEM grids.
A Brookhaven (Holtsville, NY, USA) Zeta Plus-Zeta Potential Analyzer was used to determine surface charge of nanoparticles.The stock solution was prepared by dissolving 0.037 g of potassium chloride (KCl) into 500 mL of ultrapure water to create a concentration 1 mM.200 mL of the stock solution was used and stirred during evaluation.The starting pH of stock solution was adjusted with hydrochloric acid in water (4.0 M) to reach a pH of 3. The sample of 24 mL of nanoparticles synthesized and suspended in methanol at a pH of 3 was added to the stock solution.A solution of sodium hydroxide at 0.1 M concentration was used to adjust the stock solution pH.

Conclusions
Factors that affect the particles ability to agglomerate based on the electrical double layer equation were investigated.In order to verify the inclusion of the optically active nanocrystals into transparent PMMA the molar ratio of La 3+ to Tb 3+ was confirmed by EDX analysis to be on average 5:1 and 2:1 for nanoparticles synthesized with acetylsalicylic acid in water and methanol, respectively.
Energy transfer from the ligand to the RE ion was concluded by measuring the maximum absorption of the ligand using UV-Vis absorption spectroscopy.ASA in water and in methanol produced a maximum absorption peak at 276 nm.Characteristic peaks of Tb 3+ existed in the emission spectra of nanoparticles synthesized in water and methanol upon direct ion and ligand excitation.
Nanoparticle synthesized in methanol produced agglomerate diameters about twelve times smaller than the agglomerates diameters produced in water.The methanol synthesis was chosen for further evaluation of the effect of pH on the agglomeration.
Nanoparticles synthesized in methanol have a positive surface charge and at a pH of 3 the suspension is transparent.A visual change in the suspension becomes apparent at a pH of 4. Sedimentation begins and continues at the pH range of 5 to 8. DLS analysis confirmed the agglomerate size of the nanoparticles synthesized at a pH of 3 to be on average 74 nm.Nanoparticles synthesized at a pH of 4 or higher produced agglomerates greater than the direct ion and ligand excitation wavelengths.

Figure 2 .
Figure 2. Electrical double layer distribution of ions.

Figure 4 .
Figure 4. Excitation spectra for Tb 3+ and ASA measuring the 540 nm peak.

Figure 6 .
Figure 6.(a) STEM image of polymer nanocomposite synthesis in water.Inset: agglomerate greater than 200 nm; (b) STEM image of polymer nanocomposite synthesis in methanol.
(a) shows the suspension under conventional room light and Figure 7(b) displays the luminescence of the nanoparticle suspensions when excited with ultraviolet light at the wavelength of 365 nm.The suspension is clear at the synthesis pH of 3. A change in suspension appearance is visually apparent between the synthesis pH of 3 to 4 and sedimentation occurs where large agglomerates fall out of suspension due to gravity in the suspensions synthesized at pH ≥ 5.

Figure 7 .
Figure 7. (a) Nanoparticle suspensions in methanol synthesized at different pH under conventional room light; (b) Nanoparticle suspensions in methanol synthesized at different pH under UV light at wavelength equal to 365 nm.

Figure 9 .
Figure 9. (a) TEM image of nanoparticles synthesized in methanol at a pH of 3; (b) TEM image of nanoparticles synthesized in methanol at a pH of 8.

Table 1 .
Elemental composition and corresponding atomic percentage of nanoparticles in polymethyl methacrylate (PMMA) synthesized in different solvents.

Table 2 .
Average agglomerate size of nanoparticles synthesized in water and methanol.

Table 3 .
Average particle size of agglomerates formed in methanol synthesis at various pH.