Structural and Thermal Characteristics of Buriti Tree Gum (Mauritia flexuosa)

A polysaccharide was isolated from the exudate of a buriti tree trunk (Mauritia flexuosa). The molecular structure, thermal stability, morphology, crystallinity, and elemental composition of the product were investigated through spectroscopic techniques, such as Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR 1H and 13C), and energy-dispersive X-ray spectroscopy (EDS); thermogravimetric analysis (TG), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), and X-ray diffraction (XRD). In addition to NMR molecular modeling studies, were performed to confirm the 1H and 13C chemical shifts to Gal and Xyl conformers. Buriti tree gum (BG) is an arabinogalactan, containing Rha, Ara, Xyl, and Gal, and degrades almost completely (98.5%) at 550 °C and has a maximum degradation peak at 291.97 °C, with a mass loss of 56.33%. In the temperature range of 255–290 °C, the energy involved in the BG degradation process was approximately 17 J/g. DSC indicated a glass transition temperature of 27.2 °C for BG, which had an irregular and heterogeneous morphology, with smooth or crumbling scaly regions, demonstrating the amorphous nature of BG that was confirmed by the XRD standard. EDS revealed the presence of carbon and oxygen, as well as calcium, magnesium, aluminum, silicon, chlorine, and potassium, in the BG composition.


Introduction
The Brazilian Amazon has one of the largest reservoirs of plant biodiversity on the planet, composed of a large natural collection of fruits, roots, and countless plant species, with great food, pharmaceutical, therapeutic, and chemical potentials [1]. On the other hand, many natural products used by Brazilian industries are still imported from other countries. An example is gum arabic, the main representative of the segment of natural gums used industrially [2]. It is extracted from the trunk of the Senegalese acacia tree [3]. By 2018, the polysaccharides market had raised more than USD 12 billion worldwide, while an annual growth forecast of 4.8% is expected by 2026. The food industry is largely responsible for the consumption of this plant-source material, which is produced by microbiological fermentation [4].
The buriti (Mauritia flexuosa L.), which belongs to the Arecaceae family, is an abundant palm in the mesoregion of Baixo Tocantins, in the State of Pará-Brazil, and is very important for economic extraction and subsistence, due to the use of its fruits and leaves [5]. The buriti trunk produces a gum exudate, which protects the palm from threats and microbial attacks [2,6,7]. Materials such as this contain polysaccharides with complex molecular

Gum Extraction
The exudate found on the surface of the buriti tree trunks was removed using a spatula. Approximately 1 kg of exudate was collected in Cameta (Pará, Brazil) (2 • 09 02.77 S, 49 • 26 15.57 W) and packed in polyethylene bags at room temperature (≈27 • C). BG extraction followed the methodology presented by Silva and Pena [7]. The exudate was suspended in distilled water (1:10, w/v) for 24 h at 25 • C, without stirring, to dissolve the soluble fraction. Then, the suspension was filtered through a 1 mm mesh, and the filtrate was centrifuged at 2778× g for 10 min (Suprafug 22, Heraeus Sepatech, Minden, Germany). The supernatant received a 1:3 absolute ethanol proportion (solution: ethanol, v/v). The precipitate obtained (gum) was dried at 50 • C in an air-circulating oven (Quimis Q317M72, São Paulo, Brazil) for 24 h, crushed in an analytical mill (Quimis Q298A21, São Paulo, Brazil), and packed in amber glass, hermetically sealed. The extracted BG was then stored at 25 • C until the analysis took place.

BG Infrared Spectroscopy
BG samples were kept at 25 • C, 75 • C, 100 • C, and 150 • C for 3 h, in an oven with air circulation, to check the effect of the temperature on the decomposition of the polysaccharide component of BG. After being cooled in a desiccator with silica gel, the samples were placed in Eppendorf tubes. Then, the BG samples underwent a spectroscopy analysis in the infrared region on a spectrometer (IR100 spectrometer, Thermo Electron Corporation, Madison, WI, USA). The absorption spectra were obtained in the wavelength range from 400 to 4000 cm −1 , with a resolution of 2 cm −1 and a 64 cm −1 scan [25].

NMR 1 H and 13 C BG
For NMR 1 H and 13 C, 40 mg of BG were dissolved in 4 mL of D 2 O. TSP (3-(trimethylsilyl)propionic-2,2-3,3-d 4 acid sodium salt) was used as an internal standard and the analyses were performed on a nuclear magnetic resonance spectrometer (Bruker AIII 500MHz, Billerica, MA, USA). Chemical changes were expressed in ppm (δ) [8].

BG Thermogravimetric Analysis
BG mass loss was evaluated through thermogravimetry (TG) and differential thermal analysis (DTA) in a Shimadzu thermobalance (DTG 60H, Shimadzu Analytical, and Measuring Instruments, Kyoto, Japan), with the following conditions: heating ratio of 10 • C/min, temperature range of 25-600 • C, and nitrogen atmosphere with a flow of 10 mL/min. Aluminum crucibles were used as sample holders. For the acquisition and processing of data, the OriginPro 8.0 application (Origin Lab Corporation, Microcal, Northampton, MA, USA) was used [26].

BG Differential Scanning Calorimetry (DSC)
DSC curves were obtained using equipment (DSC Q10, TA instruments, New Castle, USA) operating in the temperature range of −70-200 • C, in three stages (heating-coolingreheating), and the −10-550 • C range, with a single heating stage. In both cases, a dynamic nitrogen atmosphere was used, with a flow of 50 mL/min, and a heating rate of 10 • C/min. Aluminum crucibles were used as sample holders [26].

Scanning Electron Microscopy (SEM) and Energy-Dispersive Spectroscopy (EDS)
SEM analyses were performed using a scanning electron microscope model LEO-1430, with an EDS Sirius-SD detector attached. The analysis conditions were as follows: electron beam current of 90 µA, constant acceleration voltage of 10 kV, and a working distance of 15 mm. To visualize the BG granule morphology with greater accuracy, a TESCAN scanning electron microscope, VEGA3 model, was used [27].

X-ray Diffraction
The X-ray diffraction analysis (XRD) was performed on a diffractometer, X'PERT PRO MPD from PANalytical. Ceramic X-ray tubes and Cu anodes (Kα1 = 1.540598 Å) were used, with a long fine focus (2200 W-60 kV) and Kβ nickel filter. The analyses were performed under the following conditions: scanning from 5 • to 100 • in 2θ; voltage of 40 kV; 40 mA current; step size of 0.02 • in 2θ and 90 s time/step; divergence 1/4 • slit and 1/2 • anti spatter; 10 mm mask [28].

NMR Molecular Modeling Studies
A hypothetical molecular structure containing monomers of β-D-Galp and β-D-Xylp was designed and optimized using the DFT method at the B3LYP/cc-pVDZ level, by adjusting such molecule to a minimum local energy conformation. The minimum energy structures are guaranteed by the absence of any imaginary frequency. With all optimized molecules, the theoretical 13 C-and 1 H-NMR data were obtained by gauge-independent atomic orbital (GIAO) method [29] by applying the B3LYP/cc-pVDZ level to all atoms. To determine the TMS (internal standard) shielding constants, the same protocol used for the hypothetical molecular structure was strictly followed. All calculations were performed using the software Gaussian 16 [30]. This study was important for eliminating conformational doubts when comparing the experimental NMR data with those described in the literature for Gal and Xyl.

Gum Extraction
The buriti tree gum (BG) was isolated by using a conventional extraction method. The exudate was solubilized in distilled water at room temperature (≈25 • C) while the precipitation of the polysaccharide was performed with absolute ethanol, since the BG was not soluble in ethanol and acetone. The BG extraction yield was 5.4% while the recovery of Moringa oleifera Lam. gum was 4% using a conventional extraction process [31]. Although conventional extraction methods present lower yields compared with nonconventional methods, they have the advantage of causing less damage to the structure of polysaccharides, minimizing the loss of bioactivity in the gum [32,33].

BG Infrared Spectroscopy
The FT-IR spectrum obtained for BG is shown in Figure 1. The broad and intense band between 3600 and 3200 cm −1 is attributed to the stretching vibrations of the O-H group [34]. The band in the absorption range of 2935 cm −1 is related to the symmetrical and asymmetrical vibrations of the CH 3 and CH 2 groups [35]. The peaks at 1614 cm −1 and 1426 cm −1 are attributed to the vibrations of asymmetrical and symmetrical stretching of COO-groups, respectively [36], while the peak at 1369 cm −1 is associated with vibrations of -CH 3 and -CH 2 connections. The 1740 cm −1 , 1369 cm −1 , and 1236 cm −1 ranges are attributed to the axial deformation of the ester groups C=O, -C-CH 3 , and -C-O-, respectively [37].
adjusting such molecule to a minimum local energy conformation. The minimum energy structures are guaranteed by the absence of any imaginary frequency. With all optimized molecules, the theoretical 13 C-and 1 H-NMR data were obtained by gauge-independent atomic orbital (GIAO) method [29] by applying the B3LYP/cc-pVDZ level to all atoms. To determine the TMS (internal standard) shielding constants, the same protocol used for the hypothetical molecular structure was strictly followed. All calculations were performed using the software Gaussian 16 [30]. This study was important for eliminating conformational doubts when comparing the experimental NMR data with those described in the literature for Gal and Xyl.

Gum Extraction
The buriti tree gum (BG) was isolated by using a conventional extraction method. The exudate was solubilized in distilled water at room temperature (≈25 °C) while the precipitation of the polysaccharide was performed with absolute ethanol, since the BG was not soluble in ethanol and acetone. The BG extraction yield was 5.4% while the recovery of Moringa oleifera Lam. gum was 4% using a conventional extraction process [31]. Although conventional extraction methods present lower yields compared with nonconventional methods, they have the advantage of causing less damage to the structure of polysaccharides, minimizing the loss of bioactivity in the gum [32,33].

BG Infrared Spectroscopy
The FT-IR spectrum obtained for BG is shown in Figure 1. The broad and intense band between 3600 and 3200 cm −1 is attributed to the stretching vibrations of the O-H group [34]. The band in the absorption range of 2935 cm −1 is related to the symmetrical and asymmetrical vibrations of the CH3 and CH2 groups [35]. The peaks at 1614 cm −1 and 1426 cm −1 are attributed to the vibrations of asymmetrical and symmetrical stretching of COO-groups, respectively [36], while the peak at 1369 cm −1 is associated with vibrations of -CH3 and -CH2 connections. The 1740 cm −1 , 1369 cm −1 , and 1236 cm −1 ranges are attributed to the axial deformation of the ester groups C=O, -C-CH3, and -C-O-, respectively [37].  The range between 1200 cm −1 and 800 cm −1 represents the polysaccharide fingerprint. The absorptions in the 1033 cm −1 and 1236 cm −1 ranges are related to the presence of uronic acids and o-acetyl groups in the molecule [38]. Additionally, the 1033 cm −1 range is attributed to the vibration of C-N groups [39] and may be related to the presence of proteins in BG. Baum et al. [25] registered that the 1033 cm −1 and 1138 cm −1 ranges are attributed to the axial deformation of C-O and may be related to the presence of galacturonan, linked to the main chain of the molecule, while the 1076 cm −1 range indicates the presence of neutral sugars, such as arabinose, galactose, and xylose. The presence of xylose can also be related to the presence of 970 cm −1 and 1138 cm −1 ranges (axial deformation of C-O) [40,41]. The 820 cm −1 band refers to the stretching of the anomeric conformations of the α-D-galactopyranose polysaccharides [42]. The reduction in some ranges with the increase in the temperature was evidenced in the 3440, 2920, 1740, and 1070 cm −1 wavelengths. According to Liu et al. [43], these losses can be associated with the release of oxygen present in the functional groups of the molecule. Thus, these events can be linked to small mass losses at the studied temperatures, together with the release of water.

Hydrogen Magnetic Resonance Experimental Analysis and Molecular Modeling
When comparing the 1 H and 13 C signals from the BG residues in the NMR experimental spectroscopy (Figure 2) with the NMR data in the literature, αor βanomeric configuration information is evidenced, and the binding pattern, composition, and sequence of the monosaccharides present in BG are proposed. In the 1 H NMR experimental spectrum of BG (Figure 2a), signals 4.54, 4.71, and 5.27 ppm are attributed to anomeric protons of β-D-Galp, and residues of β-D-Xylp and α-L-Araf, respectively [44,45]. These values were confirmed in the molecular modeling studies; the chemical shifts obtained to anomeric protons were β-D-Galp (3.88 ppm) and β-D-Xylp (4.69). The values obtained are compatible with those observed in the experimental NMR, where the chemical shifts of β-D-Xylp anomeric hydrogen were greater than those of β-D-Galp. Thus, we can prove that the experimental data, the literature data, and the data obtained by molecular modeling corroborate each other.
In the 13 C NMR spectrum of BG, the C 1 resonance of β-D-Galp residues was observed at 104.3 ppm [45,46]. The 103.6 ppm signal is attributed to the anomeric carbon of β-D-Xylp [45,47]. The presence of anomeric signals 105 and 110 ppm indicates that there are Ara residues, with resonance in the furanose form [45,46]. Delgobo et al. [48]  to α-L-Rhap-(1→ residues; 3.46/75.9 ppm H 3 /C 3 and 3.77/77.1 ppm H 5 /C 5 attributed to β-D-GlcpA-(1→ residues were found in the NMR spectra for BG and compared with the results presented by Molaei and Jahanbin [45]. These results suggest that BG is an arabinogalactan with the presence of Rha, Ara, Xyl, Gal, and galacturonic acid. To certify the comparison previously made between experimental 13 C NMR data and the literature data, the results obtained by molecular modeling for the chemical shifts of the main carbon atoms of β-D-Galp and β-D-Xylp residues were compared with those assigned experimentally (Table 1). Residual analysis showed that differences between chemical shifts ranged from 0.12 to 3.85 ppm with a mean of 2.26 ppm. These values obtained are within the expected range for the use of the molecular modeling tool to confirm the structure of organic compounds, as observed in the literature [50,51]. In this way, we can verify that the structural analysis using NMR for the structure of BG is reliable.
5, x FOR PEER REVIEW 6 of 14 the structure of organic compounds, as observed in the literature [50,51]. In this way, we can verify that the structural analysis using NMR for the structure of BG is reliable.

BG Thermogravimetric Analysis
The BG TGA curve ( Figure 3) presented three stages of degradation, with a profile similar to that observed for other polysaccharides, such as gum arabic [52], guar gum [53], xanthan gum [54], and cashew gum [26]. The maximum mass loss observed was 98.52% at 527 • C. The first mass loss stage, in the 42.22 • C-110.11 • C range, was attributed to water evaporation and involved a 17.51% mass loss, with a maximum degradation temperature of 74.83 • C. The second stage occurred in the 259.75 • C-312.91 • C range and involved the greatest mass loss (56.33%), which was attributed to the degradation of the polysaccharide molecular chain [52]. For this stage, the maximum degradation temperature was 291.97 • C. The third and last stage of degradation occurred in the 402.23 • C-488.34 • C range and was attributed to complete polysaccharide decomposition [26,55]. In this stage, a 24.68% mass loss was observed, with a maximum degradation temperature of 433.69 • C.

BG Thermogravimetric Analysis
The BG TGA curve (Figure 3) presented three stages of degradation, with similar to that observed for other polysaccharides, such as gum arabic [52], guar g xanthan gum [54], and cashew gum [26]. The maximum mass loss observed wa at 527 °C. The first mass loss stage, in the 42.22 °C-110.11 °C range, was attributed evaporation and involved a 17.51% mass loss, with a maximum degradation tem of 74.83 °C. The second stage occurred in the 259.75 °C-312.91 °C range and invo greatest mass loss (56.33%), which was attributed to the degradation of the polysa molecular chain [52]. For this stage, the maximum degradation temperature wa °C. The third and last stage of degradation occurred in the 402.23 °C-488.34 °C ra was attributed to complete polysaccharide decomposition [26,55]. In this stage, a mass loss was observed, with a maximum degradation temperature of 433.69 °C. Arabinogalactans from gum arabic showed a thermogravimetric peak at which was attributed to the maximum degradation temperature of the gum [56]. ing to Al-Maqtari et al. [57], arabinogalactans show moderate temperatures of d tion. This behavior was also reported by Guan and Zhong [58], who observed the degradation of arabinogalactans from gum arabic used as a wall material in the e lation process. These results suggest that BG is more suitable to be used in app that do not involve high temperatures.

BG Differential Scanning Calorimetry
The DSC thermograms obtained for BG with 11% of moisture content on a d (db), for a heating-cooling-reheating cycle, in the temperature range of −70 °C-20 shown in Figure 4a. The thermograms define a thermal history for the polysaccha is part of BG, which did not undergo thermal degradation up to 200 °C. This b corroborates the thermogram in Figure 3, where the thermal degradation of the charide only occurs at a temperature above 250 °C. Figure 4b, in turn, shows thermogram for heating BG in the temperature range from −10 °C to 550 °C. Th peak, and final temperatures, as well as the enthalpies involved in the differen are shown in Table 2.
The first heating stage (Figure 4a) resulted in a large endothermic peak (T which can be attributed to the loss of free water from the polysaccharide [59] cooling, the heat capacity decreased monotonically with evidence of ice formation the presence of a thin peak at 0 °C, which can be attributed to the presence of resid  Arabinogalactans from gum arabic showed a thermogravimetric peak at 315 • C, which was attributed to the maximum degradation temperature of the gum [56]. According to Al-Maqtari et al. [57], arabinogalactans show moderate temperatures of degradation. This behavior was also reported by Guan and Zhong [58], who observed the thermal degradation of arabinogalactans from gum arabic used as a wall material in the encapsulation process. These results suggest that BG is more suitable to be used in applications that do not involve high temperatures.

BG Differential Scanning Calorimetry
The DSC thermograms obtained for BG with 11% of moisture content on a dry basis (db), for a heating-cooling-reheating cycle, in the temperature range of −70-200 • C, are shown in Figure 4a. The thermograms define a thermal history for the polysaccharide that is part of BG, which did not undergo thermal degradation up to 200 • C. This behavior corroborates the thermogram in Figure 3, where the thermal degradation of the polysaccharide only occurs at a temperature above 250 • C. Figure 4b, in turn, shows the DSC thermogram for heating BG in the temperature range from −10 • C to 550 • C. The initial, peak, and final temperatures, as well as the enthalpies involved in the different events, are shown in Table 2.  The first heating stage (Figure 4a) resulted in a large endothermic peak (Table 2), which can be attributed to the loss of free water from the polysaccharide [59]. During cooling, the heat capacity decreased monotonically with evidence of ice formation, due to the presence of a thin peak at 0 • C, which can be attributed to the presence of residual free water in the molecule [60].
Tg is the temperature at which a material undergoes the transformation from the gummy to the vitreous state, which is closely related to the amount of water in the gum, the gum's ability to gain (adsorb) water, and the temperature [65]. Thus, the lower Tg value observed for BG can be attributed to its higher moisture content, when compared with the moisture content of the other gums mentioned [66].
After the Tg event, an endothermic peak was observed at 96.48 • C, with enthalpy (∆H) of −49.16 J/g, in the second heating curve (Figure 4c, Table 2). The value of ∆H for this event was reduced by 88.6% when compared with the value of ∆H observed for the endothermic peak of the first heating stage. This endothermic event can be attributed to the enthalpic relaxation of BG, due to energy gain, by increasing mobility for Tg [67].
In the DSC of Figure 4b, in addition to the endothermic event at 88.02 • C, with a ∆H −417.07 J/g ( Table 2) attributed to the water evaporation of BG, three other events were observed, at temperatures above 200 • C. The second event, exothermic and with a temperature peak (T p ) of 255.4 • C, was followed by an endothermic event, with a T p very close to the previous one (257.58 • C), and, later, by another exothermic event, with a T p at 290.59 • C. The enthalpies involved in these events were approximately the same magnitude (Table 2) and can be attributed to the degradation of the polymer that is part of BG, due to the breaking of bonds between molecules [68]. Mothé and De Freitas [26] observed endothermic events at 245 • C and 360 • C for cashew gum, in an air atmosphere, with ∆H values of 142.2 and 26 J/g, respectively. The authors attributed these events to polysaccharide degradation, as well as other exothermic events related to the oxidation of carbon residues formed during the decomposition of cashew gum.
In the FT-IR spectrum (Figure 1), ranges close to 2400 cm −1 are related to the release of CO 2 [26]. In addition, the decrease in groups such as -OH (3400 cm −1 ), -CH 3 (2935 cm −1 ) and -CH 2 , and ester groups (1740 cm −1 ) with increasing temperature is a sign that these groups are involved in oxidation and rupture reactions, at temperature levels that promote BG degradation. Similar results were observed for xanthan gum and methyl cellulose [54]. Mothé and De Freitas [26] observed that in a nitrogen atmosphere, the degradation of the polysaccharide promoted the release of CO, with the degradation occurring practically in the same ranges observed for BG, which was attributed to the formation of aldehydes and alkanes due to decomposition. Thus, the presence of CO 2 and CO suggests the breakdown of the glycosidic bonds of the polysaccharide [43].

Characterization of the Microstructure and XRD Analysis
There is major variability among the microstructures of gums from different origins, but all have irregularities in their granular structures [69]. SEM images of BG powder (Figure 5a1-a3) also show the presence of very distinct and irregular structures, indicating that the BG granules have a heterogeneous morphology, with scaly and smooth regions (Figure 5a2), or even crumbling and scaly regions (Figure 5a3), similar to gum karaya, a natural gum obtained from the exudate of Sterculia urens [27,70].
During the drying process for BG (50 • C/24 h), a film was formed, which was crushed to obtain BG powder. The micrograph of the film (Figure 5b) showed a flat, homogeneous surface, with small roughness in some points and the presence of some cracks in the structure. These characteristics suggest that BG can be used in film production [71].
The irregularity of the BG microstructure, in turn, indicates the amorphous nature of the product, which is confirmed by the BG XRD standard (Figure 5d), which presented a small peak at 2θ = 21.34 • , followed by a broad plateau without peaks, a characteristic of amorphous materials. This pattern is similar to that observed for almond gum, gum arabic, and arabinogalactan [72,73].
The energy-dispersive spectroscopy (EDS) analysis, shown in Figure 5c, reveals the presence of carbon and oxygen, originating from the polysaccharide carbon chain, as well as calcium, magnesium, aluminum, silicon, chlorine, and potassium. The presence and affinity of these elements, for water or other molecules, could be fundamental for the knowledge of stability and for defining the technological uses for BG [74].
Elucidating the structure of BG can amplify the opportunities for new research aimed at the utilization of this polysaccharide in several industrial fields. One example is the use of arabinogalactans in the production of composites that are used in the development of biodegradable packaging films [13], individually or combined with carboxymethyl cellulose, methylcellulose, polyvinyl alcohol, or even nanocomposites [14][15][16][17]. Additionally, knowledge of the molecular structure of this polysaccharide and its thermal stability can assist in the development of promising materials from the BG. In the medicinal field, arabinogalactans display various health benefits, such as antitussive activity, antibody response to the pneumonia vaccine, acting as the basis for drug delivery systems, the inhibition of liver metastasis, and analgesic and anti-inflammatory properties [18][19][20].
There is major variability among the microstructures of gums from different origi but all have irregularities in their granular structures [69]. SEM images of BG powd (Figure 5a1-a3) also show the presence of very distinct and irregular structures, indicat that the BG granules have a heterogeneous morphology, with scaly and smooth regio (Figure 5a2), or even crumbling and scaly regions (Figure 5a3), similar to gum karaya natural gum obtained from the exudate of Sterculia urens [27,70]. During the drying process for BG (50 °C/24 h), a film was formed, which was crush to obtain BG powder. The micrograph of the film (Figure 5b) showed a flat, homogeneo surface, with small roughness in some points and the presence of some cracks in the str ture. These characteristics suggest that BG can be used in film production [71].

Conclusions
The gum obtained from the exudate of the buriti tree trunk was characterized. The yield extraction of polysaccharides was 5.4%. Infrared spectroscopic data indicated that BG is an arabinogalactan, containing Rha, Ara, Xyl, and Gal as well as galacturonic acid. The BG glass transition temperature was set at 27.20 • C and the maximum temperature of the greatest mass loss was 291.97 • C, attributed to the degradation of the molecule through the oxidation of the carbon residues formed. DSC revealed endo-and exothermic events between 250.5 • C and 305.87 • C, involving energies of 17.30-22.19 J/g, attributed to the loss of CO 2 and CO due to decomposition through the rupture of glycosidic polysaccharide bonds. SEM indicated that BG can be used for the production of biofilms and XRD indicated that BG is a strictly amorphous polysaccharide at room temperature. Buriti tree gum has structural and physical properties similar to gums from other plant sources that are commercially used. Its similarity might encourage further studies on this polysaccharide from this Amazonian palm tree. This is the first report on the structural and thermal characteristics of BG, and the results are important for supporting studies relating to the application of BG in the food, chemical, or pharmaceutical industries.