Removal of Heavy Metal Ions from Wastewater with Poly-ε-Caprolactone-Reinforced Chitosan Composite

Currently, the requirements for adsorbent materials are based on their environmentally friendly production and biodegradability. However, they are also related to the design of materials to sustain many cycles in pursuit of low cost and profitable devices for water treatments. In this regard, a chitosan reinforced with poly-ε-caprolactone thermoplastic composite was prepared and characterized by scanning electron microscopy; Fourier transforms infrared spectroscopy, X-ray diffraction analysis, mechanical properties, as well as erosion and swelling assays. The isotherm and kinetic data were fitted with Freundlich and pseudo-second-order models, respectively. The adsorption equilibrium capacities at pH 6 of Zn(II), Cu(II), Fe(II), and Al(III) were 165.59 ± 3.41 mg/g, 3.91 ± 0.02 mg/g, 10.72 ± 0.11 mg/g, and 1.99 ± 0.22 mg/g, respectively. The adsorbent material lost approximately 6% of the initial mass in the adsorption-desorption processes.


Introduction
Metals or metalloids such as Pb, Cd, Hg, As, Cr, Cu, Se, Ni, Ag, and Zn, as well as other metallic contaminants such as Al, Cs, Co, Mn, Mo, Sr, and U are very stable and difficult to remove by chemical or biological means. The concentration of heavy metals in the environment varies depending on the type of anthropomorphic activity such as mining, electroplating, and smelting industrial processes [1], alongside the management of these wastes to avoid leaching of the metal ions from the soil into surface and groundwater bodies [2].
One of the most commonly present ions in aqueous media is Zn which is released in the environment through the effluents of industrial activities such as galvanization, pigment formation, and the production of stabilizers, thermoplastics, alloys, and batteries [3][4][5]. Although living organisms, humans and animals, require Zn, excessive levels result in health issues and environmental damage [6,7]. In this regard, the World Health Organization (WHO) has recommended a maximum Zn concentration for drinking water of 3 mg L −1 [3,8]. According to the US EPA, the maximum limit concentration of water to preserve aquatic freshwater organisms is 120 µg L −1 , while for human health it is 7400 µg L −1 [9,10]. The European Union has registered Zn in the Dangerous Substances Directive as a List 2 set at 40 µg L −1 for estuarine and marine waters and 500 µg L −1 as drical pellets with a 1.97 ± 0.07 mm mean diameter and 6.4 ± 1.2 mm height. According to adsorption efficiencies (Appendix A), only the formulation 2:1 was used henceforth for characterization, adsorption and desorption process.

Polymers and MCP Characterization
The chemical structures of the materials were analyzed by ATR-FTIR in a Spectrum 100 (Perkin Elmer, Boston, MA, USA). The percentage of crystallinity (C%) was determined by powder X-ray diffraction (XRD) in a D8 Advance Davinci (Bruker, Billerica, MA, USA). The pH-zpc of MCP was determined by potentiometric titrations in a T50 (Mettler Toledo, Columbus, OH, USA). The tensile strength, maximum elongation at break, and Young's modulus were measured in a MTS Sintech 1/S (MTS System Corporation, Prairie, MN, USA). Elemental analysis was performed using energy-dispersive X-ray spectroscopy (EDS) (Oxford Instrument, Abingdon, UK) [20].
Microscopic analyses were carried out by treating a thin layer of the cross-section of MCP (0.5 mm) suspended in 3 mL of phosphate buffer (pH 7.2) with 30 µL of calcofluor white-reagent (CW) and vortexing for 20 s. After 2 h of reaction, samples were separated by decantation and the thin layer was observed under a microscope with fluorescent light in a microscope Axiostar plus (Carl Zeiss, Jena, Germany). Scanning electron micrographs (SEM) for MCP, CS, and PCL were obtained from a microscope JEOL JSM-5900 lv (Tokyo, Japan).
The average pore size was determined from the cross-sections of the MCP samples (six samples) and converted to 8-bit black and white images, in which the white areas represent the pores and black areas represent the solid surface. Binary images were analyzed as particles employing ImageJ 1.53a (National Institutes of Health, Bethesda, MD, USA) to determine surface porosity and average pore size (APS) [25]. The adsorption-desorption isotherm of N 2 to 77 K technique was also used to determine the MCP's specific surface area and pore volume in a Micro 200 (3P Instruments, Leipzig Germany). MCP degasification was performed for 12 h at 313.15 K. The specific surface area was calculated using Equation (1) [26], from the monolayer capacity, V a m , expressed in cm 3 (STP)/g units, a s = V a m Lσ m 2.24 × 10 22 (1) where L is the Avogadro's number and σ m is the molecular area occupied by an adsorbed N 2 molecule in the complete monolayer that commonly has a value of 0.162 nm 2 . The monolayer capacity, V a m , and the energetical parameter, C, were determined by linearization of the Brunauer-Emmett-Teller (BET) Equation (2) [26]: where V is the specific amount of N 2 adsorbed at the relative pressure (p/p 0 ) per gram of adsorbent. The erosion (E%) of MCP (29 ± 2 mg) was determined by placing 10 mL of deionized water in a flask and adding 1 N HCl until reaching a pH of 4. Composites were filtered, dried, and weighed every 24 h to determine the mass loss using Equation (3): where X 0 is the mass of the dry pellet before contact with acidic water, and X E is the mass after being in contact with acidic water. Similarly, the swelling capacity of MCP (S%) was determined by placing the sample (29 ± 2 mg) in screw-top tubes, adding 10 mL of deionized water, and placing them in a controlled bioclimatic chamber HPP260 (Memmert, Schwabach, Germany) at 25 • C with a relative humidity (RH) of 45%. MCP was collected every 24 h, placed on Whatman filter paper to remove the superficial water, and weighted (X S ). All measurements were conducted in triplicate. Equation (4) was used to calculate the amount of water retained in the pellets: Solutions with concentrations of Cu(II) (20-120 mg L −1 ), Fe(II) (15-90 mg L −1 ), and Zn(II) (70-420 mg L −1 ) were prepared at a pH of 6. Cu(NO 3 ) 2 , Pb(NO 3 ) 2 , ZnSO 4 , FeSO 4 , Cd(NO 3 ) 2 , and Al(NO 3 ) 3 were dissolved in distilled water and adjusted to a pH of 6 with NaOH (1.25 N). The calibration curves for Cu(II), Pb(II), Zn(II), Fe(II), Cd(II), and Al(III) were determined by atomic absorption spectroscopy (AAS) in a Varian 200FS (USA). Experiments were carried out in 15 mL glass flasks by mixing 0.01 g of MCP with 10 mL of metal ion solution in an orbital shaker (50 rpm) at 25 • C [27]. Experiments were halted after 120 min to ensure that equilibrium was reached. AAS determined the heavy metal concentrations in the samples, and the adsorption capacity (q t ) (mg g −1 MCP) was estimated by the Equation (5): where C 0 is the initial concentration of each heavy metal, C is the remaining heavy metal concentration in the solution after each time, v is the volume of sample, and m is the mass of MCP used in each experiment. The linearized Freundlich model (Equation (6)), the Langmuir model (Equation (7)), and the Dubinin-Radushkevich model (Equation (8)) were employed to fit the adsorption isotherms [28]: log q eq = log k F + log C eq 1 n where q eq is the equilibrium adsorption capacity (mg g −1 ), k F is the adsorption affinity constant (L mg −1 ), n represents the system's heterogeneity and C eq is the concentration of the metal ions (mg L −1 ) at equilibrium.
C eq q eq = C eq q max + 1 k L q max (7) where q max is the maximum adsorption capacity (mg g −1 ) and k L is the Langmuir constant (L mg −1 ). log q eq = log q max + k DR 2 (8) where k DR is the Dubinin-Radushkevich constant (mol 2 kJ −2 ), and is the adsorption potential and is calculated by the Equation (9): where C s is the solubility of the adsorbates (mg L −1 ), R is the gas constant (8.31 J mol −1 K −1 ) and T is the absolute temperature (298.15 K).

Adsorption Kinetic Studies
Adsorption kinetics were carried out using aqueous synthetic solutions containing heavy metal concentrations above the permissible limits according to the Mexican Official Norm-001 [29] for wastewater discharges into receptive bodies for Cu(II), Pb(II), Zn(II), Fe(II), Cd(II), and Al(III) at 10 mg kg −1 , 5 mg kg −1 , 334 mg kg −1 , 22 mg kg −1 , 1 mg kg −1 , and 4 mg kg −1 , respectively. A total of 0.01 g of MCP was mixed with 10 mL of synthetic solution in an orbital shaker (50 rpm) at 25 • C. Samples for metal ions determination were taken every 5 min for 20 min and thereafter, every 20 min until 100 min. The q t (mg g −1 ) values were calculated using the Equation (5).
Adsorption kinetic parameters were estimated by fitting the experimental data of q t to the pseudo-second order (PSO) model (Equation (10)), the Elovich model (Equation (11)) and the pseudo-first order (PFO) model (Equation (12)) [30]: where q eq and q t are the adsorption capacities at equilibrium and at time t (mg g −1 ), respectively, and k 2 (g mg −1 min −1 ) is the adsorption rate constant.
where α and β are the initial sorption rate (mg g −1 min −1 ) and the desorption coefficient which is related to the extent of surface coverage and the activation energy for chemisorption (g mg −1 ), respectively.
where k 1 is the pseudo-first order rate constant (min −1 ).

Desorption and Reusability
Acids or EDTA solutions were used to carry out heavy metal desorption. The acidic condition assays employed HNO 3 or HCl at concentrations of 0.05, 0.1, or 0.5 N [31][32][33][34]. EDTA solutions (15 g L −1 ) with NaOH 0.25 N (pH 12.8) or 0.5 N (pH 13.6) were also tested [27,35]. MCP samples from the adsorption (Section 2.4.2) were rinsed with deionized water and dried at 30 • C for 24 h. MCP (0.01 g) and 10 mL of desorption solutions were placed in 15 mL glass flasks and mixed in an orbital shaker at 50 rpm for 120 min at 25 • C. The heavy metal concentration in the desorption solutions was determined every 20 min up to 120 min by AAS, and the desorption efficiencies (ε des ) were calculated using Equation (13): where C ads is the concentration of heavy metals retained in the MCP at equilibrium (100 min) and C des is the heavy metals concentration in the desorption solutions. Six cycles were carried out for the reusability of MCP at the conditions mentioned above using for adsorption aqueous synthetic solutions (Section 2.4.2) and, for desorption, the solution with the highest ε des . The adsorption efficiency (ε ads ) at every cycle was calculated using Equation (14):

Composite Characterization
CW staining on the surface and the cross-sections ascertained the distribution of the polymers in the extruded material. Therein, CW bound to the β-1,4 glycosidic bonds of CS and fluoresced, and Figure 1 shows the sheath cluster domains on PCL.  Figure 2c). Similar behavior has been observed by Correlo et al. [36] when CS melted with polyesters showed agglomeration of CS fibers, probably due to insufficient torque during blending. In our work also, during the injection molding, several internal, tiny, and densely distributed pores were formed by aggregation and agglomeration. This influences the bulk density and mechanical properties without a significant contribution to the fluid flow [37]. The low N 2 adsorbed volume observed in the physisorption isotherms is additional evidence (Appendix B), and hysteresis and pore filling and emptying were not evident. The pore volume of vapor adsorbed, Vp, was 0.004 cm 3 g −1 and the specific surface area resulted in 2.09 m 2 g −1 at a relative pressure close to one (e.g., p/p0 = 0.98). The MCP displayed open pores having slit shapes with curved channels with one and two ends, which were larger than other closed ones. An APS of 4.68 ± 3.27 µm and a surface porosity of 5.10 ± 1.12% were estimated by image analysis of cross-section SEM micrographs (Figure 2c  The XRD pattern for CS showed two characteristic peaks at 11 and 20 • assigned to amorphous and crystalline contributions, respectively. The C% was 60.83% for this polysaccharide. The crystalline regions of PCL displayed a characteristic sharp peak at 21 • with a shoulder at 22 • and a lower intensity peak at 23 • (Figure 3). The C% of PCL was 38.7. Accordingly, an intense band at 1293 cm −1 in the FTIR corresponded to C-O-C vibrations for the crystalline PCL phase [38] (Appendix C). The XRD pattern for MCP ( Figure 3) revealed that the crystalline and amorphous domains were still present in both materials after the extrusion process. In this regard, Correlo et al. [36] reported that blends of CS and polyesters prepared by injection molding showed weak molecular interactions between the polymers with no influence on the crystalline domains. Pawar and Srivastava [39] also reported that the intensity of the characteristic peaks of PCL in the CS/PCL sponge did not change since the crystal structure of the polymers remained. Of note is that during the deacetylation process, the amine groups increased with a consequent decrease in crystallinity, which might imply an increase in the adsorption capacity, but it could also lead to mass loss due to CS solubility under acidic conditions [40]. In our work, the degree of acetylation of the CS was 7.24% representing a desirable high percentage of amine groups. However, the biological process for the production of CS [23] preserved the crystalline regions (C% = 60.83), and therefore, it might prevent excessive mass losses in the MCP at an acidic pH. The proton binding curve of MCP (Figure 4) shows that the pHzpc was 6.7, which is close to the pHzpc of CS (pH 6.3). The surface became negatively charged at pH > pHzpc, thus increasing the metal cations removal efficiency [16]. ZnSO 4 , FeSO 4 , Cd(NO 3 ) 2 , and Pb(NO 3 ) 2 dissolved in water provided positively charged metal ions, and the amine groups of MCP were still protonated at a pH of 6, exerting repulsions to a certain extent. Therefore, the electrostatic interactions between the adsorbent and adsorbates might not be relevant in the adsorption process and aid the complex formation of the divalent metal ions with amine, carboxyl, and hydroxyl groups with non-shared electron pairs. It is worth noting that according to Cu speciation at a pH of 6, only a minor fraction remained as a divalent ion, while the major was either in the neutral or solid form, i.e., a small amount adsorbed, and the rest precipitated as salt. Similarly, for Al(NO 3 ) 3 , at this pH, the metal speciation was in the passivation state (Appendix D) [41]. The FTIR spectrum for CS displays the characteristic bands at 3260 cm −1 assigned to the stretching of the amine, which correlates to the stretching movement of the carbonyl group in the amide chitin bond, 1578 cm −1 , that concurs with the vibration flexion of methyl in the acetamide group ( Figure 5). The characteristic stretching vibrators of PCL were asymmetric and symmetric CH 2 , carbonyl, and C-O-C in the crystalline phase at 2944 cm −1 , 2865 cm −1 , 1720 cm −1 , and 1293 cm −1 , respectively (Appendix C) [38]. The bands assigned to the amide and amine groups of CS did not decrease in intensity in the FTIR spectrum for MCP. This concludes that these groups did not interact with PCL during the extrusion process and remain available for the adsorption of metal ions. The FTIR spectra for MCP before and after the adsorption process (MCPA) are shown in Figure 5, which evidence the interactions among the adsorbent and adsorbates. The increase in the intensity band at 580 cm −1 is related to Cu-N and Cu-O vibrations [42]. Additionally, the bending vibrations of the amino group at 1559 cm −1 decreased and displaced 9 cm −1 after the adsorption process, indicating its involvement in the metal complex formation. The secondary OH of C3 (1076 cm −1 ) and the primary OH of C6 (1027 cm −1 ) related to Cu-O in MCPA were overlapped by bands assigned to PCL. Of note is that the two main mechanisms, bridge and pendant, described for CS-Cu complexation involve two and one nitrogen ligands, respectively [31,43]. Rhazi et al. [44] demonstrated that the formation was more for the pendant than the bridge model, meaning a lower dissociation and greater stability. For the CS-Fe complex, a similar mechanism was described by Bhatia & Ravi [45], where two nitrogen ligands and two oxygen ligands from different CS units were involved in complexation. They also proposed that two more oxygen ligands in the media could stabilize this complex. The appearance of a slight shoulder peak at ca. 1650 cm −1 could imply an interaction between the oxygen atom of the PCL carbonyl and the metal ions [46]. According to the literature [41,[47][48][49] and considering that the copper, zinc, and iron elements are in the divalent form, the adsorption mechanism shown in Figure 5 proposes two nitrogen ligands of parallel CS chains and oxygen, the latter either from PCL, or hydroxyl anions at the alkaline media.
The MCP had a Young's modulus of 212.60 ± 73.69 MPa, a tensile strength of 6.75 ± 0.69 MPa, and a maximum elongation at break of 101.95 ± 0.82%. The tensile strength for the extruded blends of CS with polyester presented relatively low values. A plausible explanation is the immiscibility and inherent incompatibility between CS and polyester, as reported by Correlo et al. [36] with chitosan and polyester blends.
The swelling of the MCP reached equilibrium after 24 h with 85.77%, and afterward, the amount of retained water remained constant (p > 0.05) (Appendix E). In a previous report by Wu [22] and Bikiaris et al. [50], high contents of CS in melted blends with polyesters tended to increase water absorption. This was primarily due to the presence of amine and hydroxyl moieties in the CS and their bonding to the water molecules. Nonetheless, a good adsorption capacity could be related to a higher number of available free amine groups, which could also be related to an increased metal ion adsorption capacity [31]. Finally, it is worth mentioning that the erosion of MCP did not change significantly (p > 0.05) after 120 h, even though a pH of 4 favors CS dissolution (Appendix E).

Adsorption Kinetics and Equilibrium
The empirical (Freundlich), chemical (Langmuir), and Polanyi's potential theorybased models (Dubinin-Radushkevich) were fitted to the adsorption data ( Figure 6). These models are extensively used to describe sorption reactions of solutes [28]. The equation of Freundlich had a better fit for Cu and Zn (Figure 6), revealing that the adsorbent surface is heterogeneous in a multi-layer adsorption mechanism [1,51]. While the adsorption data for Fe are well-fitted by the Langmuir model, this implies that chemisorption occurred in a monolayer filling the outer interface of MCP ( Figure 6). Previous studies conducted with chitosan and modified chitosan-based materials as heavy metal adsorbents also reported that data fitted to both Langmuir and Freundlich isotherms indicate that mono-and multi-layer adsorptions may have occurred [30]. There is also a multi-layer physisorption mechanism for Zn into MCP, supported by the high R 2 of the fitting to the Dubinin-Radushkevich model (Figure 6), describing pore filling [52]. The initial and final pH remained without significant changes for Zn, while Cu became slightly acidic from a pH of 6.15 ± 0.05 to 5.67 ± 0.19. For Fe, the pH changed from 6.03 ± 0.09 to 6.65 ± 0.10, close to pHzpc (Appendix F). At a pH of 6, Al(III) precipitated rather than adsorbed as it is in the predicted species distribution curves (Appendix C). The order of MCP affinity was Zn and Cu based on the estimated k F , while comparing q max, the adsorption of MCP was higher for Zn than Fe ( Figure 6).  Figure 7 shows the adsorption kinetics for Zn, Fe, Cu, and Al into MCP. Only, in the beginning, MCP adsorbed Pb(II) and Cd(II), which might be due to the competition between metal ions for active sites in CS [1]. After 60 min of treatment, MCP reached the maximum adsorption capacity with almost all the metal ions in the sample. The PSO model fitted better the adsorption data of Cu(II), Zn(II), Fe(II), and Al(III) than Elovich or PFO (R 2 ≤ 0.9) (Figure 7). The limiting step might be the chemisorption at a certain extent with complexation to the surface, which involves valency forces through the sharing or the exchange of electrons between the adsorbent and adsorbate in an ion-exchange adsorbent/adsorbate mechanism [51,53]. The PSO model estimated q eq values which are close to the q eq values determined experimentally (Figure 7). It is worth mentioning that the estimation of the q eq for each metal ion in a complex mixture solution showed the competition for the functional groups in MCP. The highest q eq was for Zn (159.25 mg g −1 ) and was 38-fold, 14.7-fold, and 82-fold higher than for Cu, Fe, and Al, respectively (Figure 7). Chen et al. [54] reported a decrease in the adsorption capacity of Zn in the presence of other metal ions in a eucalyptus leaf-based magnetic biosorbent. Herein, the Zn adsorption capacity into MCP was higher than those obtained by other CS-based materials previously reported ( Table 1). The affinity and compatibility of CS and PCL with Zn(II) either or both adsorbent or doped materials are well known [21,46,[55][56][57][58]. Another plausible explanation for the high adsorption capacity of Zn(II) into MCP is the ion size. Zn has a small size and can diffuse across the inner MCP layers and pores faster than Cd and Pb with larger sizes, occupying the available groups (Section 3.1).

Desorption and Reusability
The addition of an alkaline EDTA solution favors the formation of highly stable, soluble complexes, thereby desorbing Cu and Fe from MCP. The results suggest that some metal ions precipitated and others might have been chelated by the EDTA molecules ( Figure 8) [61]. For zinc ions, anionic species such as HZnO 2 − are formed that also could be chelated by EDTA [41,[47][48][49]. On the other hand, the complete desorption of copper might be ascribed to the formation of the bridge complex with CS, which resulted in less stability and easier dissociation [44]. However, the Zn and Fe ions were not completely desorbed after 120 min with EDTA/NaOH 0.5 N and corroborated with the Zn and Fe concentrations in the MCP, which were 46.93 ± 0.08 mg kg −1 and 0.50 ± 0.04 mg kg −1 , respectively. According to Krężel & Maret [48] and Pearson [62], based on the classification of Lewis acids and bases, Zn(II) and Fe(II) are borderline acids with a tendency to form stable complexes with moderately polarizable ligands such as the nitrogen donors from CS. It could explain the difficulty of complete desorption. The proposed adsorption mechanism among CS, Fe, Zn was the formation of complex coordination sites with up to six or seven ligands [45]. From the ε des shown in Figure 9, after 120 min of treatment, almost 100% of the Cu(II) desorbed with both EDTA solutions (Section 2.5). The 0.5 N concentration of NaOH allowed for a more efficient desorption process (p < 0.05) than that with 0.25 N, which contrasts with Wang et al. [27], who proved that a low concentration of NaOH was desirable for full desorption. The ε des with HCl (0.5 N) reached the highest values of 53.43 ± 9.72%, 11.96 ± 1.94%, and 41.81 ± 12.05% for Fe, Zn, and Al, respectively. Nonetheless, the E% after the desorption process in acidic conditions (HCl 0.5 N) was also the highest, representing a disadvantage for the MCP reusability. In the case of the desorption process with HNO 3 , 0.05 N and 0.5 N achieved the complete desorption of Fe without MCP mass loss. Nonetheless, MCP lost its original form and destroyed the pellet, confirming that the adsorption mechanism was mainly due to electrostatic interactions [1]. The highest ε des for Zn was 8.6 ± 1.53% and for Al was 3.23 ± 0.2% with 0.1 N. The ε des values with acidic solutions are shown in Appendix G. The contaminated MCP with adsorbed heavy metal ions was exposed to a fresh EDTA/NaOH 0.5 N solution and left in agitation for another 2 h to achieve a full desorption of Zn(II). After four changes of the desorption solution, the ε des achieved was 79.19 ± 0.21% for Zn(II) from the contaminated MCP (Appendix H). The gradient formation resulted in a higher desorption efficiency with the addition of a fresh desorption solution.
The reusability of MCP was proven by adding MCP in synthetic solution for an adsorption cycle, and subsequently, desorption was carried out with an EDTA/NaOH 0.5 solution for a total of six cycles (Figure 10). High εads values were observed in the first cycle for Zn(II), Fe(II), Al(III), and Cu(II), which agree with the kinetic studies previously discussed (59.4 ± 1.2%, 62.9 ± 1.5%, 43.4 ± 4.3%, and 38.9 ± 1.2% respectively). The first desorption cycle achieved the release of 100% of the Cu(II) and Al(III) adsorbed in the MCP. Zn(II) and Fe(II) only desorbed 80% and 68%, respectively. In further reuse cycles, the ε ads values of Zn(II), Fe(II), and Cu(II) decreased considerably possibly due to the incomplete desorption of MCP, owing to MCP's high affinity for these ions. Surprisingly, from the second cycle, the ε ads values of Pb(II) and Cd(II) rose 43.3 ± 6.8% and 62.3 ± 2.1%, respectively, and remained constant until completion of the six cycles. For CS-metal ion complexes studied for the adsorption of other metal ions [63], it was reported that the adsorption efficiency improved because the CS-metal complex acted as a hard base with an affinity for hard acids such as Pb(II) and Cd(II), which explains the adsorption of these ions by Zn(II) and Fe(II) adsorbed in MCP after the first desorption cycle.

Elemental Analysis by EDS
SE micrographs using backscattered electrons signed along with their X-ray diffraction patterns for MCP samples before and after the adsorption treatment showed the main elemental composition of MCP (Appendix I). CS, PCL, and MCP, before the adsorption, were mainly composed of carbon and oxygen. After the adsorption, MCP's composition changed, and Al and Fe were also detected in the CS fraction, which corroborated the adsorption role of CS in the MCP.

Conclusions
The understanding of the adsorption mechanism of a mixture of metal ions in water at a pH near the point of zero charge (pHzpc) of the adsorbent composite for reducing electrostatic interactions and metal competition for active groups for chelation led to the improvement of the adsorption process. MCP presents a high selectivity for Zn (165.59 ± 3.41 mg g −1 ) in comparison with other CS-based materials. In addition, the material is capable of removing metal ions from water. Adsorbed heavy metals were successfully desorbed, and six cycles of adsorption-desorption were achieved. Desorbed MCP remains effective for adsorbing metal ions and improves its performance since Pb and Cd were retained after the first adsorption-desorption cycle. Therefore, this composite was able to remove several metal ions in a continuous system and desorb them. This experimental evidence leads to the conclusion of the successful utilization of MCP in several effluent cycles for the removal of metal contaminants. The research in this field finds common grounds in the design of CS-derived and reusable adsorbent materials as an economically attractive, environmentally friendly, and profitable path.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.

Acknowledgments:
The authors wish to thank Heriberto Alonso Gómez and Ricardo Rosas who are greatly acknowledged for their assistance in Atomic Absorption Spectrometry and XRD analyses, respectively, at the Universidad Autónoma Metropolitana-Iztapalapa. We also thanks Mariana Ramírez Gilly for her technical support with mechanical property measurements at the Universidad Nacional Autónoma de México.

Conflicts of Interest:
The authors declare no conflict of interest.

Appendix A
Appendix A shows the adsorption efficiency of the MCP formulations, CS and PCL for 2 h at an agitation of 5 rpm with 10 mL of synthetic solution ( Figure A1).

Appendix B
Appendix B shows the N 2 physisorption isotherms of MCP ( Figure A2).

Appendix C
Appendix C shows the FTIR spectrum of PCL ( Figure A3).

Appendix H
Appendix H ( Figure A8) shows the desorption efficiency of Zn(II) ions changing the desorption solution for fresh EDTA/NaOH 0.5 N volume. Figure A8. The desorption efficiency of Zn(II) ions changing the desorption solution for fresh EDTA/NaOH 0.5 N volume.