Introduction of Re(CO)3+/99mTc(CO)3+ Organometallic Species into Vinylpyrrolidone-Allyliminodiacetate Copolymers

N-vinylpyrrolidone-co-allylamine copolymers (VP-co-AA) containing iminodiacetic (IDA) chelation units were prepared in the range of molecular masses of the copolymers from 9000 to 30,000 Da depending on polymerization conditions. Non-radioactive organometallic species Re(CO)3+ were introduced into polymeric carriers under mild conditions; the prepared metal–polymeric complexes were characterized by IR, NMR, ESI-MS and HPLC. IR spectra data confirmed the coordination of M(CO)3+ moiety to the polymeric backbone via IDA chelation unit (appearance of characteristic fac-M(CO)3+ vibrations (2005, 1890 cm−1), as well as the appearance of group of signals in 1H NMR spectra, corresponding to those inequivalent to methylene protons CH2COO (dd, 4.2 ppm), coordinated to metal ions. The optimal conditions for labeling the PVP-co-AA-IDA copolymers with radioactive 99mTc(CO)3+ species were determined. The radiochemical yields reached 97%. The obtained radiolabeled polymers were stable in blood serum for 3 h. In vivo distribution experiments in intact animals showed the high primary accumulation of technetium-99m MPC (MM = 15,000 Da) in blood with subsequent excretion via the urinary tract.


Introduction
The chemistry of synthetic metal-polymer complexes (MPCs) is an urgent and vast area of polymer science. MPCs find wide application in various fields of science and engineering: the analysis and fixation of heavy metal ions, the design of biosensors, electro-optical devices, and antimicrobial, antibacterial, antiviral, antifungal, and antitumor activities [1][2][3]. MPCs are macromolecules containing metal ions, which, as a rule, are linked with polymer chains via chelation units [4,5]. In this respect, the use of metal-ligand interactions is versatile in this context, since the interaction strength and dynamic nature of the complexes can, in principle, be tuned by the variation of the metal ions, ligands, and linkers. Systems with various architectures can be formed, and, thus, polymers could be considered as irregulated macromolecular chelation systems [5].
The target delivery of radiolabeled compounds to specific sites of the human body is an important task of modern medicinal chemistry. Technetium-99m ( 99m Tc, t 1/2 = 6 h, E = 141 keV) is the most commonly used radiometal for nuclear medical diagnostics [6][7][8].
In combination with 186 and 188 isotopes of its heavier homolog rhenium (186/188Re), technetium represents one of the first "theranostic pairs" for nuclear medical diagnosis In this work, we present the synthetic method for introducing the IDA chelation unit into copolymer carriers (vinylpyrrolidone-co-allylamine (VP-co-AA)). The synthesis of metal-polymeric complexes (MPCs) of the non-radioactive analog of 99m Tc (Re(CO) 3 ) was carried out; structures of the products were studied, molecular masses and related characteristics were estimated; the technique for the chromatographic analysis of MPCs involving the use of monolithic sorbents was developed, and the procedure for labeling copolymers with radioactive 99m Tc(CO) 3 + organometallic species was suggested.

Instruments and Measurements
1 H and 13 C NMR spectra were recorded using a Bruker Avance II-500 WB spectrometer (Billerica, USA) in deuterated solvent (D 2 O) purchased from Sigma-Aldrich, (St Louis, MO, USA). Chemical shifts were referenced to the signal of residual non-deuterated solvent (water, 4.8 ppm) and the signal of external tetramethylsilane standard.
IR spectra were obtained using a Shimadzu Prestige FTIR spectrometer (Kyoto, Japan) in KBr pellets.
Mass spectra were obtained with a Bruker APEX-Qe ESI FT-ICR instrument (Billerica, MA, USA).
Chromatographic analysis was performed with the use of a Smartline HPLC instrument (Knauer, Geretsried, Germany) equipped with a JetStream column thermostat, refractometric and spectrophotometric detectors. The registration of chromatograms and calculations of molecular masses and other parameters were performed using Clarity-Chrom GPC/SEC V.2.6 xx setup (Geretsried, Germany). An Ultrahydrogel linear SEC column (7.8 × 300 mm) with a pre-column (0.6 × 40 mm, Waters, Milford, MS, USA) was used for the analysis of the copolymers. Chromatographic analyses were carried out in aqueous solution of 0.2 M NaCl at 25 • C. Calibration dependences for the columns were plotted using the data for the previously characterized poly(N-vinylformamide) standards in 0.2 M aqueous solution of NaCl; the values of the Kuhn-Mark-Houwink constants were K = 10.74 × 10 -3 and α = 0.76 ± 0.04 [35]. Ultrashort monolith CIM TM (Convection Interaction Media) columns (CIM disks, 1.2 × 0.5 cm) (Ajdovščina, Slovenia) were used for monitoring the polymer-metal interaction and for the evaluation of radiochemical yields.
Intrinsic viscosity [η] was measured using an Ubbelohde viscometer, (Vekton, St Petersburg, Russia). Relative viscosity (η r ) was calculated as an initial slope of the ln(η r ) = f(c) dependence, i.e., in the region where η r is the relative viscosity of a solution at concentration c. The measurements were performed in 0.1 M solution of sodium acetate at 25 • C.
The 99m Tc eluate was purchased from the Khlopin Radium Institute (St Petersburg, Russia). Radioactivity was measured using a Curie mentor 3/4 dose calibrator and a GabiStar specialized chromatographic flow detector (Raytest, Straubenhardt, Germany). Reactions of free radical copolymerization of VP and AA were carried out in sealed ampoules in argon atmosphere at 60 • C in ethanol or water solutions for 48 h. Concentrations of monomers were 50 wt.%, and the concentration of the initiator (2,2-azobisisobutironitrile, AIBN) was 1 wt.% with respect to monomer mass. The polymers obtained in ethanol solution were precipitated into diethyl ether and dialyzed through a 1000-MWCO (molecular weight cutoff) dialysis tubing (Spectra/Por 7, New Brunswick, NJ, USA) against 2% NaCl water solution for 24 h and against water for 24 h. Then, the copolymers were lyophilized. The copolymers synthesized in water solutions were dialyzed and lyophilized.
Compositions of the copolymers were determined by two methods: (1) using the absorption spectra of complexes of AA units and 2,4,6,-trinitrobenzenesulfonic acid, λ max = 420 nm, (2) by potentiometric titration of AA units with 0.1 N HCl solution. Molecular masses (MMs) of copolymers were estimated viscometrically using the Mark-Kuhn-Houwink parameters found for PVP. The values of intrinsic viscosity (η) were determined in 0.1 M Na 2 SO 4 solution (25 • C).
The reaction between polymer amino groups and monochloroacetic acid was carried out in KOH water solution (pH = 10) at 90 • C [34,36]. The content of IDA-containing groups was estimated by potentiometric titration. The reaction schemes, monomer ratios, intrinsic viscosities, and SEC data are presented in Scheme 1 and in Tables 1 and 2. Reactions of free radical copolymerization of VP and AA were carried out in sealed ampoules in argon atmosphere at 60 °C in ethanol or water solutions for 48 h. Concentrations of monomers were 50 wt.%, and the concentration of the initiator (2,2-azobisisobutironitrile, AIBN) was 1 wt.% with respect to monomer mass. The polymers obtained in ethanol solution were precipitated into diethyl ether and dialyzed through a 1000-MWCO (molecular weight cutoff) dialysis tubing (Spectra/Por 7, New Brunswick, NJ, USA) against 2% NaCl water solution for 24 h and against water for 24 h. Then, the copolymers were lyophilized. The copolymers synthesized in water solutions were dialyzed and lyophilized.
Compositions of the copolymers were determined by two methods: (1) using the absorption spectra of complexes of AA units and 2,4,6,-trinitrobenzenesulfonic acid, λmax = 420 nm, (2) by potentiometric titration of AA units with 0.1 N HCl solution. Molecular masses (MMs) of copolymers were estimated viscometrically using the Mark-Kuhn-Houwink parameters found for PVP. The values of intrinsic viscosity (η) were determined in 0.1 M Na2SO4 solution (25 °C).
The reaction between polymer amino groups and monochloroacetic acid was carried out in KOH water solution (pH = 10) at 90 °C [34,36]. The content of IDA-containing groups was estimated by potentiometric titration. The reaction schemes, monomer ratios, intrinsic viscosities, and SEC data are presented in Scheme 1 and in Tables   The organometallic precursor [ 99m Tc (CO) 3 (H 2 O) 3 ] + was prepared by reducing 99m TcO 4 − eluate (T 1/2 = 6 hrs, E γ = 140 keV) (1 mL, average activity: 1-2 GBq) with Na 2 BH 3 CO 2 in the presence of sodium-potassium tartrate at 90 • C for 10 min according to the procedure described elsewhere [37,38]. Then, the reaction mixture was neutralized to pH = 6.5 with phosphate buffer solution.
The in vitro stability of VP-co-AA-IDA-99m Tc(CO) 3 (MM 30 kDa) was evaluated as follows. An amount of 150 µL of solution of rat blood serum in 0.1 M NaCl was added to 50 µL of solution of 99m Tc-labeled copolymer in phosphate buffer (pH = 7.4). The mixture was incubated at 37 • C for 15-180 min. The stability of the labeled copolymer in blood serum was estimated by HPLC on Ultrahydrogel Linear SEC column (0.78 × 30 cm), elution: 0.2 M NaCl, 0.8 mL/min.

Biodistribution Studies
The procedure was as described previously [34]. The biodistribution of the MPC in organisms of linear intact laboratory Wistar rats (body weight about 200 g) was studied according to the following protocol. A 99m Tc(CO) 3 -VP-AIDA MPC(15 kDa) (1-3 MBq, 0.25 mL), adjusted to pH 7.4 with phosphate buffer system, was injected into the caudal vein. After injection, the animals were sacrificed by decapitation. The target organs, blood (0.5 g), and tissues samples (0.1-0.6 g) and blood samples were placed in tubes with the same geometry. The radioactivity measurements of the 68 Ga were performed using a calibrated gamma meter by direct radiometry. The accumulation of the MPC in organs and tissues was calculated as a percentage of the total introduced activity per 1 g of an organ/tissue. Since the 99m Tc radioisotope is short-lived (the half-life is 6 h, which is comparable with the time of experiment), this factor was taken into account when recalculating the accumulation in organs and tissues. Prior to measuring the radioactivity of a series of organs and tissues, the radioactivity of the reference sample of the same MPC was measured in the tube of the same geometry. The measurement time of the standard sample and selected organs and issue was equal. The measured radioactivity was accepted as the total activity for a specific series, taking into account measurement time.

Results and Discussion
Copolymers of N-vinylpyrrolidone were selected as carriers for metal radionuclides due to their biocompatibility and cell membrane permeation properties [12]. It is known Polymers 2021, 13, 1832 7 of 15 that molecular masses of polymers used in pharmaceutical preparations should not exceed 40-50 kDa, and their hydrodynamic radii should lie in the range of 5-10 nm to permit renal or hepatobiliary excretion [11,12]. Thus, the directional design of copolymers with the desired chemical and structural characteristics (MM, polydispersity, hydrodynamic properties) is important for the target transport of radionuclides.
It should be noted that lactone rings of poly-N-vinylpyrrolidone (PVP) homopolymer contain donor atoms (N, O), which potentially can bind metal ions. Therefore, firstly, we studied the possibility of complexation between Re(CO) 3 + organometallic compound and PVP (MM = 30 kDa) by HPLC on ultrashort monolith columns (carboxymethyl modification). The use of monolithic sorbents, which are able to separate both homopolymers in a gradient mode and metal ions [33,34], may be a possible solution of a complex problem of separation of flexible-chain BASP and metal ions.
Monitoring the reaction at various pH values and temperatures demonstrated the absence of any noticeable coordination activity of PVP.
In order to impart complexation ability to PVP, its copolymers with directionally attached iminodiacetic (IDA) chelating units were synthesized. The synthetic pathway is presented in Scheme 1.
Copolymers of VP with AA were obtained by radical copolymerization in ethanol solution (where the total concentration of monomers was equal to 50 wt.%) using azobisisobutyronitrile (AIBN, 1 wt.%) as an initiator. It is known that AA shows relatively low reactivity in copolymerization with VP [36], and an increase in the AA content in the monomer mixture leads to a decrease in MM of the resulting copolymer. Thus, we synthesized VP-AA copolymers with contents of AA units varying from 6 to 12 mol.%; their Mη ranged from 9000 to 30,000 Da (Table 2), which meets the requirements for polymer radiolabeling and target drug transport.
IDA chelation groups were introduced into the polymeric backbone by the alkylation of amino groups of the VP-AA copolymer with monochloracetic acid (Scheme 1).
Contents of amino groups in the resulting VP-AA samples were determined by UV spectroscopy at the wavelength of 420 nm (which is characteristic for complexes between primary amines and 2,4,6-trinitrobenzenesulfonic acid). Contents of carboxylic groups in VP-co-AIDA copolymers were evaluated by potentiometric titration of a sample with 0.1 N solution of HCl. MM of copolymers were calculated using the SEC data (Table 1) (eluent: 0.1 M aqueous solution of NaCl, 25 • C). The calibration dependence was plotted using Mark-Kuhn-Houwink constants for poly-N-vinylformamide [35].
As can be seen, no significant changes in MWD between starting co-polymers and resulting MPCs were observed. The same tendency was observed previously [33,34] using more precise methods, e.g., molecular hydrodynamics and optics in combination with SEC. This fact suggests the hypothesis, that the introduction of metals into the macromolecular matrix will not significantly disturb its hydrodynamical and biological properties and, thus, should not drastically disturb its biological behavior.
Rhenium was chosen for the experiments, since it is a closest chemical analogue of technetium. In order to develop the technique for the radiochemical preparation of MPC at the non-carrier added level (where metal ion concentration is equal to 10 −10 -10 −13 M), it is necessary to optimize synthetic conditions for the procedures involving noticeable quantities of the polymer carrier and metal ions, to determine the composition and structure of the target product and to optimize chromatographic techniques. The latter is especially important, since chromatography is the main method for the determination of radiochemical yields of radiolabeled compounds. The reaction between organometallic [Re(CO) 3 (H 2 O) 3 ] + precursor and VP-co-AA-IDA was carried out at room temperature for 48 h or at 70 • C for 4 h.
Reaction kinetics was monitored by HPLC (the yield of the metal-polymer complex was estimated). In order to reduce the time of analysis, monolith ultra-short columns were used. Due to unusual the pore structure and small length (0.3 cm) of monolith columns, they make it possible to perform fast and effective separations of high and low molecular weight compounds (5-10 min). The latter advantage is critical for radiochemical synthesis, since diagnostic radionuclides have short half-lives. However, CIM monolith columns cannot separate polymers according to their molecular masses, because the sorption mechanism is realized in the column during the chromatographic experiment (i.e., a polymer is adsorbed on the matrix).
Various derivatives of the basic material (glycidyl methacrylate/ethylenediamine dimethacrylate, GMA-EDMA), including strong cation and anion exchangers (DEAE, quaternary amine, SO 3 , carboxymethyl) were tested. Strange as it may seem, the carboxymethyl (CM) stationary phase was found to be the most effective in separation of a macromolecular component from an organometallic moiety in organic-aquatic media.
A typical HPLC profile of the reaction mixture [Re(CO 3 )] + + VP-co-AIDA (MM ≈ 15 kDa) is presented in Figure 1. It should be noted that the UV spectrum of the starting copolymer includes the characteristic peak at a wavelength of 210 nm. An additional absorption maximum at 360 nm is present in the UV spectra of the resulting MPC, as well as in the spectra of aqueous [Re(CO) 3  they make it possible to perform fast and effective separations of high and low molecular weight compounds (5-10 min). The latter advantage is critical for radiochemical synthesis, since diagnostic radionuclides have short half-lives. However, CIM monolith columns cannot separate polymers according to their molecular masses, because the sorption mechanism is realized in the column during the chromatographic experiment (i.e., a polymer is adsorbed on the matrix).
Various derivatives of the basic material (glycidyl methacrylate/ethylenediamine dimethacrylate, GMA-EDMA), including strong cation and anion exchangers (DEAE, quaternary amine, SO3, carboxymethyl) were tested. Strange as it may seem, the carboxymethyl (CM) stationary phase was found to be the most effective in separation of a macromolecular component from an organometallic moiety in organic-aquatic media.
A typical HPLC profile of the reaction mixture [Re(CO3)] + + VP-co-AIDA (MM ≈ 15 kDa) is presented in Figure 1. It should be noted that the UV spectrum of the starting copolymer includes the characteristic peak at a wavelength of 210 nm. An additional absorption maximum at 360 nm is present in the UV spectra of the resulting MPC, as well as in the spectra of aqueous [Re(CO)3(H2O)3] + . The appearance of absorbance at this wavelength in the HPLC profile of the macromolecular component clearly indicates the formation of MPC.
The retention time (RT) of macromolecular components is close to the free volume of the column, while the peak of [Re(CO)3(H2O)3] + complex has a higher RT. The shape of wide peak (2) can be explained by the rapid exchange of labile water molecules in the coordination spheres of [Re(CO)3(H2O)3] + particles and the sorption of these particles on the surface of the sorbent.  The retention time (RT) of macromolecular components is close to the free volume of the column, while the peak of [Re(CO) 3 (H 2 O) 3 ] + complex has a higher RT. The shape of wide peak (2) can be explained by the rapid exchange of labile water molecules in the coordination spheres of [Re(CO) 3 (H 2 O) 3 ] + particles and the sorption of these particles on the surface of the sorbent.
The reaction mixture was purified by dialysis against water, and the target MPC was freeze-dried. Coordination between [Re(CO 3 (Figure 2)).
The reaction mixture was purified by dialysis against water, and the target MPC was freeze-dried. Coordination between [Re(CO3)] + and VP-co-AIDA was confirmed by IR spectroscopy (strong characteristic bands of fac-[Re(CO3)] + moiety (2017, 1880 cm −1 ) was appeared and significantly shifted in contrast to reference [Re(CO)3Br3][N(Et)4]2 (2000, 1863 cm −1 ) (Figure 2)). 1 H NMR spectra of VP-AIDA copolymers is practically the same as PVP, because in general, the most intensive signals are attributed to the PVP backbone. Signals of AIDA protons are underplayed with more intense peaks of the PVP backbone. The same effect was observed earlier in the case of analogous polymers VP-VIDA [27].
However, in the 1 H NMR spectra of metal-polymer complexes, downfield shifts of the signals attributed to AIDA methylene protons (2.89 ppm, (m)) linked to the polymeric backbone were observed. In addition, the fine structure of the signals of methylene N-CH2-CO2 protons (dd, 3. 4 ppm)) becomes inequivalent upon coordination to metal core. The same effect occurring in 1 H NMR spectra of similar metal-polymer complexes Ga-VP-VIDA has been previously described [27]. The coupling of signals of methylene protons was also observed in 1 H NMR spectra of low molecular weight complex [Re(CO)3(IDA)][N(Et)4] [34].
The MM of polymers and MPC were determined by electrospray mass-spectrometry (ESI MS). The unique ability of ESI to produce multiply charged ions extends this extremely soft technique to a higher mass range, even for analyzers with a limited mass range. Unfortunately, even polymers with low polydispersities and high masses give very complex mass spectra due to multiple charging [39,40]. Although mass spectrometry gives the exact molecular weights of individual molecules, accurate molecular weight distributions (MWD) of synthetic polymers can only be obtained in some instances. This is because 1 H NMR spectra of VP-AIDA copolymers is practically the same as PVP, because in general, the most intensive signals are attributed to the PVP backbone. Signals of AIDA protons are underplayed with more intense peaks of the PVP backbone. The same effect was observed earlier in the case of analogous polymers VP-VIDA [27].
However, in the 1 H NMR spectra of metal-polymer complexes, downfield shifts of the signals attributed to AIDA methylene protons (2.89 ppm, (m)) linked to the polymeric backbone were observed. In addition, the fine structure of the signals of methylene N-CH 2 -CO 2 protons (dd, 3. 4 ppm)) becomes inequivalent upon coordination to metal core. The same effect occurring in 1 H NMR spectra of similar metal-polymer complexes Ga-VP-VIDA has been previously described [27]. The coupling of signals of methylene protons was also observed in 1 H NMR spectra of low molecular weight complex [Re(CO) 3

(IDA)][N(Et) 4 ] [34].
The MM of polymers and MPC were determined by electrospray mass-spectrometry (ESI MS). The unique ability of ESI to produce multiply charged ions extends this extremely soft technique to a higher mass range, even for analyzers with a limited mass range. Unfortunately, even polymers with low polydispersities and high masses give very complex mass spectra due to multiple charging [39,40]. Although mass spectrometry gives the exact molecular weights of individual molecules, accurate molecular weight distributions (MWD) of synthetic polymers can only be obtained in some instances. This is because samples of synthetic polymers do not have uniform chain lengths, but demonstrate a distribution of molecular weights. A representative ESI mass-spectrum of VP-co-AIDA-[Re(CO 3 )] metal-polymer complex is presented in Figure 3. It should be noted that the shape of ESI MS mass distribution generally resembles the SEC profile of a sample. The overall charge of macromolecular fractions is 10 + , and this value does not depend on MM; besides, the introduction of Re(CO 3 ) does not have any significant influence on mass distribution. samples of synthetic polymers do not have uniform chain lengths, but demonstrate a distribution of molecular weights. A representative ESI mass-spectrum of VP-co-AIDA-[Re(CO3)] metal-polymer complex is presented in Figure 3. It should be noted that the shape of ESI MS mass distribution generally resembles the SEC profile of a sample. The overall charge of macromolecular fractions is 10 + , and this value does not depend on MM; besides, the introduction of Re(CO3) does not have any significant influence on mass distribution.   samples of synthetic polymers do not have uniform chain lengths, but demonstrate a distribution of molecular weights. A representative ESI mass-spectrum of VP-co-AIDA-[Re(CO3)] metal-polymer complex is presented in Figure 3. It should be noted that the shape of ESI MS mass distribution generally resembles the SEC profile of a sample. The overall charge of macromolecular fractions is 10 + , and this value does not depend on MM; besides, the introduction of Re(CO3) does not have any significant influence on mass distribution.   The main goals of the present work were to develop an effective procedure for the radiolabeling of VP-co-AA-IDA with radioactive 99m Tc(CO) 3 + moiety, and to achieve high radiochemical yields and high degrees of purity necessary for biodistridution studies. It is known that the reactivity of Tc carbonyls is higher than that of Re analogs [24]. The following optimal conditions for the radiolabeling reaction were found: 80 • C, 20 min, and the copolymer concentration equal to 10 −3 M.
The reaction yields were evaluated by HPLC on CIM columns with weak ion exchanging carboxymethyl phase in the isocratic mode. It was shown that the maximum yield is achieved at pH 8; then, a decrease in the reaction yield was observed ( Figure 5).
The small yield of the reaction at low pH can be explained by the protonation of the IDA carboxylic groups, which reduces their reactivity. Meanwhile, strongly alkaline media facilitate the substitution of one water molecule in 99m Tc(CO) 3 (H 2 O) 3 + with hydroxyl ion and the formation of a weakly reactive complex 99m Tc(CO) 3

(H 2 O) 2 (OH).
is known that the reactivity of Tc carbonyls is higher than that of Re analogs [24]. The following optimal conditions for the radiolabeling reaction were found: 80 °C, 20 min, and the copolymer concentration equal to 10 −3 M.
The reaction yields were evaluated by HPLC on CIM columns with weak ion exchanging carboxymethyl phase in the isocratic mode. It was shown that the maximum yield is achieved at pH 8; then, a decrease in the reaction yield was observed ( Figure 5). The small yield of the reaction at low pH can be explained by the protonation of the IDA carboxylic groups, which reduces their reactivity. Meanwhile, strongly alkaline media facilitate the substitution of one water molecule in 99m Tc(CO)3(H2O)3 + with hydroxyl ion and the formation of a weakly reactive complex 99m Tc(CO)3(H2O)2(OH). Similar dependences were observed for samples with molecular masses equal to 9000 Da and 30,000 Da; thus, it can be concluded that the radiolabeling yield does not depend on molecular mass of the copolymer.
A typical HPLC profile of the reaction mixture is shown at Figure 6 (pH 5.5). Resolved peaks of radiolabeled polymer and starting 99m Tc(CO)3 species could be observed. The highest radiochemical yield was equal to 95-97% (pH 8) ( Figure 4); this product can be used in biological studies without additional purification.
According to the HPLC data, the target 99m Tc(CO)3-VP-co-AIDA metal-polymer complexes were stable in aqueous solutions at room temperature at pH values ranging from 2 to 9 for at least 24 h.
The stability of the 99m Tc(CO)3-VP-co-AIDA complex in vitro was assessed by the incubation of the complex in the blood serum of rats for 15-180 min at 37 °C (Figure 7). The mixture was analyzed by HPLC, which made it possible to separate serum proteins (RT varying from 6.0 to 9.4 min) and the metal-polymer complex (RT 9.8 to 9.9 min). It was shown that the 99m Tc(CO)3-VP-co-AIDA metal-polymer complex is sufficiently stable for 3 h. Similar dependences were observed for samples with molecular masses equal to 9000 Da and 30,000 Da; thus, it can be concluded that the radiolabeling yield does not depend on molecular mass of the copolymer.
A typical HPLC profile of the reaction mixture is shown at Figure 6 (pH 5.5). Resolved peaks of radiolabeled polymer and starting 99m Tc(CO) 3 species could be observed. The highest radiochemical yield was equal to 95-97% (pH 8) ( Figure 4); this product can be used in biological studies without additional purification.  According to the HPLC data, the target 99m Tc(CO) 3 -VP-co-AIDA metal-polymer complexes were stable in aqueous solutions at room temperature at pH values ranging from 2 to 9 for at least 24 h.
The stability of the 99m Tc(CO) 3 -VP-co-AIDA complex in vitro was assessed by the incubation of the complex in the blood serum of rats for 15-180 min at 37 • C (Figure 7). The mixture was analyzed by HPLC, which made it possible to separate serum proteins (RT varying from 6.0 to 9.4 min) and the metal-polymer complex (RT 9.8 to 9.9 min). It was shown that the 99m Tc(CO) 3 -VP-co-AIDA metal-polymer complex is sufficiently stable for 3 h. Figure 6. HPLC profile of the reaction mixture containing VP-co-AIDA copolymer (15,000 Da) and 99m Tc(CO)3 species (reaction mixture at pH 5.5) (CIM CM column 0.5 cm × 1 cm); 0.1% TFA/acetonitrile, 0.3 mL/min (red-UV detection at 210 nm, yellow-γ-radioactivity detection).

Biodistribution Studies
Biodistribution of MPC 99m Tc(CO) 3 -VP-AIDA (MM = 15,000 Da) in intact laboratory animals (Wistar rats) was investigated (Figure 8. It could be seen from the presented data that in the case of intact animals, the radiolabeled MPC is primarily accumulated in the bloodstream (30 min) (3.1% of the injected dose per 1 g of a tissue (ID/g (%)) and in the urinary tract (kidney (0.86%) and bladder (0.9%)). It is noteworthy that the active substance is rather rapidly (in 60 min after injection) excreted through the urinary tract (kidney (3.2%) and bladder (5.2%)). The noticeable accumulation of the radioactive substance in liver was also observed ((0.83% (30 min), (0.61% (60 min)). At the same time, the very slow accumulation of activity in muscle tissues was observed (0.21% (30 min), 1,2% (60 min). bloodstream (30 min) (3.1% of the injected dose per 1 g of a tissue (ID/g (%)) and in the urinary tract (kidney (0.86%) and bladder (0.9%)). It is noteworthy that the active substance is rather rapidly (in 60 min after injection) excreted through the urinary tract (kidney (3.2%) and bladder (5.2%)). The noticeable accumulation of the radioactive substance in liver was also observed ((0.83% (30 min), (0.61% (60 min)). At the same time, the very slow accumulation of activity in muscle tissues was observed (0.21% (30 min), 1,2% (60 min).

Conclusions
Novel complexes of rhenium tricarbonyl with copolymers of N-vinylpyrrolidone and allyliminodiacetate with molecular masses suitable for the realization of the EPR effect were synthesized under mild conditions and characterized by several physico-chemical methods. The coordination of Re(CO)3 moiety to polymer matrix was confirmed by spectral studies. The combination of IR and 1 H NMR spectroscopy allowed to confirm the introduction of M(CO)3 + moieties into the VP-AIDA polymeric backbone. A fast and efficient procedure for chromatographic separation of macromolecular and organometallic components using ultrashort monolith columns was proposed; the technique is suitable for fast and effective radiochemical analysis. The achieved radiochemical yields of the target MPC were sufficiently high for biological studies; their specific activity was revealed. The prepared MPCs were stable in rat blood serum for 3 h. The biodistribution of the sample of 99m Tc(CO)3 -VP-AIDA with MM 15,000 Da in laboratory animals (Wistar rats) demonstrated relatively fast clearance via urinary traction, but the growth of activity accumulation in muscle tissues makes it a promising object for the further development of novel synthetic polymeric carriers for target chemistry.

Conclusions
Novel complexes of rhenium tricarbonyl with copolymers of N-vinylpyrrolidone and allyliminodiacetate with molecular masses suitable for the realization of the EPR effect were synthesized under mild conditions and characterized by several physico-chemical methods. The coordination of Re(CO) 3 moiety to polymer matrix was confirmed by spectral studies. The combination of IR and 1 H NMR spectroscopy allowed to confirm the introduction of M(CO) 3 + moieties into the VP-AIDA polymeric backbone. A fast and efficient procedure for chromatographic separation of macromolecular and organometallic components using ultrashort monolith columns was proposed; the technique is suitable for fast and effective radiochemical analysis. The achieved radiochemical yields of the target MPC were sufficiently high for biological studies; their specific activity was revealed. The prepared MPCs were stable in rat blood serum for 3 h. The biodistribution of the sample of 99m Tc(CO) 3 -VP-AIDA with MM 15,000 Da in laboratory animals (Wistar rats) demonstrated relatively fast clearance via urinary traction, but the growth of activity accumulation in muscle tissues makes it a promising object for the further development of novel synthetic polymeric carriers for target chemistry.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.