Selenonium Polyelectrolyte Synthesis through Post-Polymerization Modifications of Poly (Glycidyl Methacrylate) Scaffolds

Atom transfer radical polymerization of glycidyl methacrylate monomer with poly(ethylene glycol)-based macroinitiators leads to the formation of reactive block copolymers. The epoxide side-chains of these polymers can be subjected to a regiospecific base-catalyzed nucleophilic ring-opening reaction with benzeneselenol under ambient conditions. The ß-hydroxy selenide linkages thus formed can be alkylated to access polyselenonium salts. 77Se-NMR indicates the formation of diastereomers upon alkylation. In such a manner, sequential post-polymerization modifications of poly(glycidyl methacrylate) scaffolds via selenium-epoxy and selenoether alkylation reactions furnish practical access to poly(ethylene glycol)-based cationic organoselenium copolymers.


Introduction
Cationic polyelectrolytes find various bio-relevant applications due to their ability to interact with oppositely charged biomolecules. Typically, ammonium, sulfonium, and phosphonium groups are used to endow the polymer chain with a positive charge. We have recently shown that selenium atoms appended to a polymer chain can be transformed into a cation to afford the first known family of selenonium polyelectrolytes [1]. The synthesis was carried out through a selenium-epoxy reaction [2]. In this reaction, diselenide precursors (R-Se-Se-R) were reduced in-situ with the help of sodium borohydride to generate selonolate (R-Se -) nucleophiles. Such nucleophiles can open the epoxide rings to produce ß-hydroxy selenide linkages [3][4][5][6][7][8][9][10][11][12]. A subsequent alkylation of the produced seleno-ethers then affords the side-chain selenonium cations. The reaction conditions, however, can be considered as relatively harsh since sodium borohydride is a strong reducing agent. Therefore, in this work we investigate milder conditions to achieve the synthesis of organoselenonium polyelectrolytes. In addition, new polymer compositions (for instance, copolymer architectures containing poly(ethylene glycol)s) are sought to establish the generality of the concept and to enhance the repertoire of the newly introduced polycation family. The described polymers provide an alternative to the known families of polycations (e.g., ammonium, phosphonium, and sulfonium polymers) and are anticipated to be useful in a range of biological studies [13].
In this post-polymerization functionalization [17][18][19][20][21][22] study, the proton resonances from the epoxide group (labeled with filled circles in Figure 1, Figures S1-S8) are followed as a function of reaction time, reaction medium, and the nature and amount of a base to fathom the extent of the ring-opening reaction [23][24][25]. Initially, the reactions were carried out for 12 h with 3.6 mol% of catalyst. In addition, since oxidative dimerization of benzeneselenol to diphenyldiselenide (Ph-Se-Se-Ph) under ambient conditions is a known side-reaction [2], an excess of 2 (1.25 eq./epoxide) was used. Under these conditions, in tetrahydrofuran (THF) and chloroform, the extent of ring-opening reaction remained unsatisfactory (65-84%, Table 1). Only in aqueous THF, LiOH led to a quantitative ring-opening reaction. Therefore, the amount of the catalyst was increased to 5.9 mol%. In these cases, DBU and LiOH both produced a quantitative ring-opening reaction in THF. In chloroform, however, DBU and TEA only resulted in a 91% ring-opening reaction. LiOH worked equally well in both solvent systems, resulting in the quantitative transformation of the side-chain epoxides to the seleno-ethers. Finally, the reaction time was reduced to 3 and 1 h. These reactions indicated that 1 h of reaction time was insufficient for all systems and provided only a 55-86% ring-opening reaction. The reaction time of 3 h, however, was sufficient for LiOH to provide a quantitative reaction in both solvent systems. Therefore, LiOH can be considered as the best catalyst that can be used in chloroform or aqueous THF in a loading of 5.9 mol% to furnish 3 (M w(GPC) = 52,400, M n(GPC) = 42,500, M w /M n = 1.2) in 3 h. DBU can be used as an alternative in 5.9 mol% loadings to furnish 3 in THF in 12 h of reaction time. Gel permeation chromatography (GPC) indicated that the functionalization reactions did not alter the polymer dispersity in a significant manner, and that the hydrodynamic volume increased due to the addition of the phenyl ring to the polymer side-chain ( Figure 2). Based on these results, 5.9 mol% of LiOH in aqueous THF was chosen along with the reaction time of 3 h for future ring-opening reactions on the planned block copolymer scaffolds.
The regiochemistry of the ring-opening reaction could be verified by acetylation of the hydroxyl group ( Figure 3) [1]. Upon acetylation, resonance from one proton shifted downfield to approximately 5 ppm. This indicated that only one proton was located adjacent to the hydroxyl group. It meant that the base-generated selenolate nucleophile attacked the least substituted carbon atom and produced a secondary hydroxyl group in the system. A lack of any other signals suggested that this isomer formed exclusively and the base-catalyzed ring-opening reaction was regiospecific.
Encouraged by the optimization study, the synthesis of block copolymers was targeted [26,27]. 2) block copolymers [28]. The methylene groups from the poly(ethylene glycol) backbone could be observed at 3.5 ppm in 1 H-NMR spectra of the block copolymers (Figure 4 and Figure S9). An area integration analysis suggested that polymers 6 and 7 contained about 185 and 210 average number of glycidyl units, respectively. These groups could be functionalized with benzeneselenol to furnish organoselenium polymers 8 (M w(GPC) = 36,100, M n(GPC) = 28,000, M w /M n = 1.2) and 9 (M w(GPC) = 50600, M n(GPC) = 38,300, M w /M n = 1.3) under the optimized conditions. Once again, the functionalization reactions did not affect the polymer dispersity in an adverse manner, and a change in retention time to lower values is observed after the polymer functionalization process (Figures S10 and S11).    The bottom shows a model compound of known regiochemistry prepared through selenium-halide reaction between bromopropane diol and benzeneselenol followed by acetylation [2]. The residual solvent signals from DMSO and water are shown with the help of an asterisk.
Next, alkylation of the side-chain selenium atoms was considered. For this, methyl iodide and pentyl iodide were chosen as the alkylating agents (Scheme 3). As can be seen in Figure 5, the transformation of selenium to an electron-deficient selenium cation results in a downfield shift of the aromatic signals and adjacent methylene protons. In the case of 10, the newly introduced methyl group can be seen at 3 ppm, while in the case of 11, the aliphatic chain could be observed in the range of 0.5-2 ppm. The area integration analyses in both cases indicate a complete ionization of the structures.    Finally, the synthesized polymers were studied with the help of 77 Se-NMR spectroscopy. In this analysis, diphenyldiselenide was used as a standard with a chemical shift of 463 ppm [29]. This study indicated that neutral selenium atoms in polymers 3, 8, and 9 resonate at 267 ppm ( Figure 6). However, once the polymers become cationic, a large downfield shift (> 110 ppm) is observed due to the electron-poor nature of the cationic selenium species in polymers 10 and 11. Furthermore, in the case of ionic structures, two signals could be observed due to the formation of diastereomers in the system (Figure 7).

Conclusions
In summary, base-catalyzed selenium-epoxy reaction is an efficient method to prepare organoselenium polymers. An optimization study on poly(glycidyl methacrylate) indicated that LiOH is the best catalyst as it can be used in chloroform as well as aqueous THF in 5.9% loadings to quantitatively open the side-chain epoxides in 3 h of reaction time. These conditions can be successfully applied to AB and ABA-type block copolymers containing a poly(ethylene glycol) segment. Finally, the selenium atoms can be alkylated to afford cationic polymers. In essence, this study establishes the use of selenol nucleophiles in the ring-opening reaction of polymeric glycidyl scaffolds and shows the generality of the selenonium polyelectrolyte structures by preparing (polyethylene glycol)-containing AB and ABA-types of block copolymers.