Removal of Linear and Branched Alkylphenols with the Combined Use of Polyphenol Oxidase and Chitosan

Removal of linear and branched alkylphenols with different alkyl chain lengths or different branchings (normal, secondary, and tertiary), some of which are suspected as endocrine disrupting chemicals, from an aqueous medium were investigated through quinone oxidation by polyphenol oxidase (PPO) and subsequent quinone adsorption on chitosan beads or powders at pH 7.0 and 40 °C. PPO-catalyzed quinone oxidation increased with an increase in alkyl chain length of the alkylphenols used. Although a higher PPO dose was required for quinone oxidation of branched alkylphenols, they were completely or mostly removed by quinone adsorption on chitosan beads or powders. The apparent activity of PPO increased by a decrease in quinone concentration. On the other hand, in the homogeneous systems with solutions of chitosan and PPO at pH 6.0, longer reaction times were required to generate insoluble aggregates, and a small amount of quinone derivatives were left in the solution even under optimum conditions. These results support that the two-step reaction, that is, PPO-catalyzed quinone oxidation and subsequent quinone adsorption on chitosan beads or powders, in the heterogeneous system is a good procedure for removing linear and branched alkylphenols from aqueous medium.


Introduction
Interference exerted by an exogenous substance in the hormonal system is referred to as endocrine disruption, and the substances themselves are known as endocrine-disrupting chemicals (EDCs). EDCs and potential EDCs are found in various materials such as pesticides, additives, or contaminants in food and personal care products. EDCs are also called as xenoestrogens. They mean "foreign" estrogens that have estrogenic effects on a living organism, even though they differ chemically from estrogenic substances internally produced by the endocrine system of an organism. Bisphenol A (BPA) and its derivatives-phthalates, alkylphenols, phytoestrogens, dichloro-diphenyltrichloroethane (DDT), polychlorinated biphenyls (PCBs), and dioxins-are representative xenoestrogens with similar structures to 17β-estradiol in EDCs [1,2].
Alkylphenols have been used as raw materials of alkylphenol polyethoxylate-type nonionic surfactants, antioxidants for plastics, and heat stabilizers for polyvinyl chloride, and they frequently exist in surface waters [3,4]. Alkylphenol ethoxylates undergo degradation by the stepwise loss of ethoxy groups to form shorter ethoxylate homologs, ethoxycarboxylates, and ultimately alkylphenols. Alkylphenols with an alkyl side chain on the para position exhibit weak estrogen-like actions, and para-alkylphenols with longer alkyl chains show higher estrogen receptor binding affinities [5,6].  3 4TOP 0.10 7.5 vol% -(CH 2 ) 8 CH 3 4NNP 0.10 31.5 vol% (1) The DMSO concentration in the reaction solution.

Removal of Alkylphenols in the Heterogeneous Reaction
Stock solutions of alkylphenols and PPO were prepared in a pH 7.0 phosphate buffer (ionic strength = 0.01 M) at prescribed concentrations [33,34]. Chitosan beads were washed with buffer several times and then preserved in buffer before adsorption experiments. In a 50 cm 3 Erlenmeyer flask, a PPO solution was added to each alkylphenol solution of 30 cm 3 , and then the solutions were diluted with buffer to a final volume of 40 cm 3 to achieve the initial alkylphenol concentrations shown in Table 1. The initial concentration was adjusted to 0.30 mM for alkylphenols with an alkyl chain length of 1 to 6 [17,20,21,23]. The initial concentration of 4-n-hexylphenol (4NHexP) was adjusted to 0.20 mM because the solubility of 4NHexP in a pH 7.0 buffer was low. For 4-n-heptylphenol (4NHepP), 4-n-octylphenol (4-NOP), 4-tert-octylphenol (4TOP), and 4-n-nonylphenol (4NNP), the initial concentration was adjusted to 0.10 mM by using a pH 7.0 buffer containing DMSO. DMSO concentration in these reaction solutions is shown in Table 1.
When the UV-visible spectra of the reaction solutions were measured over the reaction time, the peak appeared at 400 nm. Therefore, absorbance at 400 nm was used as a measure of quinone generation [17,20,21,23]. For removal experiments, 4.0 cm 3 of chitosan beads were added to each alkylphenol solution with a bullet, and then the enzymatic reaction was initiated by adding PPO to the solutions [20][21][22][23]. Three kinds of chitosan powders were used in place of chitosan beads for quinone adsorption experiments. Since chitosan beads of 4.0 cm 3 in the swollen state consisted of 0.30 g chitosan and 3.70 g water, according to the water content value, 0.30 g of chitosan powders was dispersed in alkylphenol solutions containing PPO.

Quantitative Assay of Alkylphenols
The concentration of alkylphenols remaining in the solutions was determined by Hitachi L-7000 high-performance liquid chromatography (HPLC) combined with a UV-visible spectrophotometer and an integrator (Hitachi L-7420) [17,20,21,23]. A reverse phase column, Inertsil ODS-2 (particle size: 5 µm, 4.6 mm i.d. × 15 cm), was used, and the composition of the aqueous acetonitrile solution as a mobile phase was adjusted so as to set the retention time of each alkylphenol between 7 and 9 min. A solution of precisely 20 mm 3 was injected into the HPLC system with a microsyringe, and the mobile phase ran at 1.0 cm 3 /min. The conversion percent value was calculated from the peak areas before the enzymatic reaction and at time t according to Equation (1).

Removal of Alkylphenols in the Homogeneous System
Homogeneous removal experiments were performed as a control. Chitosan solutions of 1.0-1.5 w/v% were prepared by dispersing chitosan powders in distilled water and intermittently adding 2 M HCl solution dropwise with constant stirring for at least 24 h to maintain a pH value of 3-4. Then, insoluble parts were removed by vacuum filtration with a G3 glass filter. The obtained chitosan solutions were diluted with distilled water to an amino group concentration of 0.15-30 mM based on the weight concentration of the chitosan solution and the degree of deacetylation of the used chitosan powder [22]. The stock solutions of alkylphenols and PPO were prepared in a pH 6.0 phosphate buffer (ionic strength = 0.01 M) at prescribed concentrations.
To the solutions of butylphenols and pentylphenols, the solutions of PPO and chitosan were added so as to reach an alkylphenol concentration of 0.30 mM. The molar ratio of chitosan's amino group to alkylphenol ranged from 0.05 to 10.0, which corresponded to an amino group concentration of 0.015-3.0 mM. The solutions were stirred at 40 • C, and the absorbance of the solutions was measured at predetermined time intervals. In the case where insoluble aggregates were generated in the solution, the absorbance of the filtrates was measured after removal with a 5C filter paper.

Polyphenol Oxidase (PPO) Concentration Dependence of Quinone Oxidation
Effects of enzyme concentration on PPO-catalyzed quinone oxidation of 14 kinds of linear and branched alkylphenols were investigated at pH 7.0 and 40 • C (Table 1). These values were determined as the optimum conditions of PPO used in this study for BPA and its derivatives in our previous articles [33,34]. Therefore, these optimum conditions were used for PPO-catalyzed quinone oxidation in this study. Figure 1 shows changes in the conversion percent value at a reaction time of 60 min as well as the initial velocity of conversion into the corresponding catechols with the PPO concentration for PPO-catalyzed quinone oxidation of linear alkylphenols with alkyl chain lengths of 1 to 6. Quinone oxidation of these linear alkylphenols increased with an increase in PPO concentration irrespective of the alkyl chain length. The initial velocity of conversion into the corresponding catechols, or the cresolase activity, was proportional to the PPO concentration. The decrease in alkylphenol concentration meant that each alkylphenol was converted into the corresponding catechols by PPO in the presence of molecular oxygen [27,28,35]. In addition, the initial velocity linearly increased against the PPO concentration. M HCl solution dropwise with constant stirring for at least 24 h to maintain a pH value of 3-4. Then, insoluble parts were removed by vacuum filtration with a G3 glass filter. The obtained chitosan solutions were diluted with distilled water to an amino group concentration of 0.15-30 mM based on the weight concentration of the chitosan solution and the degree of deacetylation of the used chitosan powder [22]. The stock solutions of alkylphenols and PPO were prepared in a pH 6.0 phosphate buffer (ionic strength = 0.01 M) at prescribed concentrations.
To the solutions of butylphenols and pentylphenols, the solutions of PPO and chitosan were added so as to reach an alkylphenol concentration of 0.30 mM. The molar ratio of chitosan's amino group to alkylphenol ranged from 0.05 to 10.0, which corresponded to an amino group concentration of 0.015-3.0 mM. The solutions were stirred at 40 °C, and the absorbance of the solutions was measured at predetermined time intervals. In the case where insoluble aggregates were generated in the solution, the absorbance of the filtrates was measured after removal with a 5C filter paper.

Polyphenol Oxidase (PPO) Concentration Dependence of Quinone Oxidation
Effects of enzyme concentration on PPO-catalyzed quinone oxidation of 14 kinds of linear and branched alkylphenols were investigated at pH 7.0 and 40 °C (Table 1). These values were determined as the optimum conditions of PPO used in this study for BPA and its derivatives in our previous articles [33,34]. Therefore, these optimum conditions were used for PPO-catalyzed quinone oxidation in this study. Figure 1 shows changes in the conversion percent value at a reaction time of 60 min as well as the initial velocity of conversion into the corresponding catechols with the PPO concentration for PPO-catalyzed quinone oxidation of linear alkylphenols with alkyl chain lengths of 1 to 6. Quinone oxidation of these linear alkylphenols increased with an increase in PPO concentration irrespective of the alkyl chain length. The initial velocity of conversion into the corresponding catechols, or the cresolase activity, was proportional to the PPO concentration. The decrease in alkylphenol concentration meant that each alkylphenol was converted into the corresponding catechols by PPO in the presence of molecular oxygen [27,28,35]. In addition, the initial velocity linearly increased against the PPO concentration. The appearance of the absorbance at 400 nm showed that the alkylphenols used underwent PPOcatalyzed quinone oxidation in the same manner as the case when mushroom and Aspergillus tyrosinases were used [20][21][22][23]33,34]. This meant that the catechol intermediates were converted into the corresponding quinone derivatives by the catecholase activity of PPO. In addition, the absorbance more slowly increased against the reaction time than the conversion percent value did against the reaction time. Quinone oxidation of alkylphenols by PPO occurred by a two-step enzymatic reaction: cresolase activity to oxidize a phenol compound into the corresponding catechol compound and catecholase activity to oxidize the catechol compound into the corresponding quinone derivative [36,37]. Since catechols are colorless, a decrease in alkylphenol concentration against the reaction time is referred to as the velocity of conversion of alkylphenols into the corresponding catechols. Thus, it was found from the above results that the velocity of conversion into catechols by cresolase activity was higher than that of conversion into the corresponding quinone derivatives by catecholase activity.
The use of enzymes with higher reaction specificities is desirable for the treatment of phenol compounds by an oxidoreductase. We reported that an increase in alkyl chain length of alkylphenols, or between two phenol groups of BPA derivatives, resulted in an increase in enzyme dose for the treatment of alkylphenols [17,20,21,23] or BPA and its derivatives [18,19,22] by mushroom tyrosinase [20,22], Aspergillus oryzae tyrosinase [23], horseradish peroxidase [17,18], and soybean peroxidase [19]. The specific initial velocity of PPO used in this study increased from 1.14 × 10 −3 μmol/min･g for p-cresol to 4.29 × 10 −3 μmol/min･g for 4NHexP. Here, it should be noted that n-alkyl phenols with alkyl chain lengths of 1 to 6 underwent quinone oxidation at lower concentrations than mushroom tyrosinase and Aspergillus oryzae tyrosinase [17,20,23].
Next, quinone oxidation of branched alkylphenols was investigated at pH 7.0 and 40 °C. Figure  2 shows changes in the conversion percent value at 60 min and the initial velocity with PPO concentrations for 4-iso-propylphenol (4IProP), 4-sec-buthylphenol (4SBP), 4TBP, and 4TPenP. Quinone oxidation of these branched alkylphenols also increased with PPO concentration in the same manner as the corresponding linear alkylphenols. In addition, comparison of Figures 1 and 2 showed that a higher dose of PPO was required for quinone oxidation of branched alkylphenols than for that of corresponding linear alkylphenols. The initial velocities for linear alkylphenols were lower than that for branched alkylphenols, for example, 4-n-propylphenol (4NProP) > 4IProP and 4-npentylphenol (4NPenP) > 4TPenP. For butylphenols, the initial velocity for 4-n-buthylphenol (4NBP) had the highest value and was followed by 4SBP and 4TBP (4NBP > 4SBP > 4TBP). These results revealed that PPO catalyzed quinone oxidations of these branched alkylphenols at lower concentrations than mushroom tyrosinase [20,23]. The appearance of the absorbance at 400 nm showed that the alkylphenols used underwent PPO-catalyzed quinone oxidation in the same manner as the case when mushroom and Aspergillus tyrosinases were used [20][21][22][23]33,34]. This meant that the catechol intermediates were converted into the corresponding quinone derivatives by the catecholase activity of PPO. In addition, the absorbance more slowly increased against the reaction time than the conversion percent value did against the reaction time. Quinone oxidation of alkylphenols by PPO occurred by a two-step enzymatic reaction: cresolase activity to oxidize a phenol compound into the corresponding catechol compound and catecholase activity to oxidize the catechol compound into the corresponding quinone derivative [36,37]. Since catechols are colorless, a decrease in alkylphenol concentration against the reaction time is referred to as the velocity of conversion of alkylphenols into the corresponding catechols. Thus, it was found from the above results that the velocity of conversion into catechols by cresolase activity was higher than that of conversion into the corresponding quinone derivatives by catecholase activity.
The use of enzymes with higher reaction specificities is desirable for the treatment of phenol compounds by an oxidoreductase. We reported that an increase in alkyl chain length of alkylphenols, or between two phenol groups of BPA derivatives, resulted in an increase in enzyme dose for the treatment of alkylphenols [17,20,21,23] or BPA and its derivatives [18,19,22] by mushroom tyrosinase [20,22], Aspergillus oryzae tyrosinase [23], horseradish peroxidase [17,18], and soybean peroxidase [19]. The specific initial velocity of PPO used in this study increased from 1.14 × 10 −3 µmol/min·g for p-cresol to 4.29 × 10 −3 µmol/min·g for 4NHexP. Here, it should be noted that n-alkyl phenols with alkyl chain lengths of 1 to 6 underwent quinone oxidation at lower concentrations than mushroom tyrosinase and Aspergillus oryzae tyrosinase [17,20,23].
Next, quinone oxidation of branched alkylphenols was investigated at pH 7.0 and 40 • C. Figure 2 shows changes in the conversion percent value at 60 min and the initial velocity with PPO concentrations for 4-iso-propylphenol (4IProP), 4-sec-buthylphenol (4SBP), 4TBP, and 4TPenP. Quinone oxidation of these branched alkylphenols also increased with PPO concentration in the same manner as the corresponding linear alkylphenols. In addition, comparison of Figures 1 and 2 showed that a higher dose of PPO was required for quinone oxidation of branched alkylphenols than for that of corresponding linear alkylphenols. The initial velocities for linear alkylphenols were lower than that for branched alkylphenols, for example, 4-n-propylphenol (4NProP) > 4IProP and 4-n-pentylphenol (4NPenP) > 4TPenP. For butylphenols, the initial velocity for 4-n-buthylphenol (4NBP) had the highest value and was followed by 4SBP and 4TBP (4NBP > 4SBP > 4TBP). These results revealed that PPO catalyzed quinone oxidations of these branched alkylphenols at lower concentrations than mushroom tyrosinase [20,23]. In addition, H2O2 is required for quinone oxidation of 4TBP by mushroom tyrosinase [27], whereas PPO-catalyzed quinone oxidation occurs without H2O2. Figure 3 shows changes in the conversion percent value at 60 min and the initial velocity with PPO concentrations for 4NHepP, 4NOP, 4TOP, and 4NNP at pH 7.0 and 40 °C. These alkylphenols also underwent quinone oxidation by PPO without H2O2. In particular, it was found that quinone oxidation of 4TOP was much slower than that of 4NOP.  In addition, H 2 O 2 is required for quinone oxidation of 4TBP by mushroom tyrosinase [27], whereas PPO-catalyzed quinone oxidation occurs without H 2 O 2 . Figure 3 shows changes in the conversion percent value at 60 min and the initial velocity with PPO concentrations for 4NHepP, 4NOP, 4TOP, and 4NNP at pH 7.0 and 40 • C. These alkylphenols also underwent quinone oxidation by PPO without H 2 O 2 . In particular, it was found that quinone oxidation of 4TOP was much slower than that of 4NOP. In addition, H2O2 is required for quinone oxidation of 4TBP by mushroom tyrosinase [27], whereas PPO-catalyzed quinone oxidation occurs without H2O2. Figure 3 shows changes in the conversion percent value at 60 min and the initial velocity with PPO concentrations for 4NHepP, 4NOP, 4TOP, and 4NNP at pH 7.0 and 40 °C. These alkylphenols also underwent quinone oxidation by PPO without H2O2. In particular, it was found that quinone oxidation of 4TOP was much slower than that of 4NOP.

PPO-Catalyzed Quinone Oxidation and Subsequent Quinone Adsorption on Chitosan Beads
Since it was found in the above section that the linear and branched alkylphenols used were successfully quinone-oxidized by PPO without H 2 O 2 , quinone oxidation by PPO was performed in the presence of chitosan beads for their adsorptive removal. Figures 1-3 also showed changes in the conversion percent value at 60 min and the initial velocity with the PPO concentration in the presence of chitosan beads at 0.10 cm 3 /cm 3 (shaded plots). Solutions of these alkylphenols turned brown by enzymatic quinone generation in the absence of chitosan beads. On the other hand, in the presence of chitosan beads, an increase in the absorbance was highly suppressed by adsorption of enzymatically generated quinone derivatives on the chitosan beads [20,22,23]. This indicated that these alkylphenols were removed by quinone adsorption on chitosan beads. Quinone adsorption occurs through a chemical reaction of enzymatically generated quinone derivatives with chitosan's amino groups. This mechanism was explained in detail in ours and Payne's articles [20,22,23,26]. Results of removing the 14 kinds of alkylphenols used are summarized in Table 2. Quinone oxidation in the presence of chitosan beads was faster than in the absence of chitosan beads, and the alkylphenol concentration decreased at shorter reaction times. Also, these alkylphenols were completely or mostly quinone-oxidized in the presence of chitosan beads. Absorbance slightly increased in the beginning of the reaction and was followed by a decrease to zero or nearly zero by adsorption on the chitosan beads. As a result, the alkylphenols used were highly removed at a reaction time of 3 to 5 h. Many articles described that when quinone derivatives enzymatically generated from various phenol compounds reacted with amino groups of chitosan, a peak appeared at the wavelength of about 420 nm. Chitosan films immersed in the solutions containing a phenol compound and PPO turn dark-brownish. On the other hand, a cellulose film did not stain at all [20,23,26,27,33,34]. This indicated that the enzymatically generated quinone derivatives successfully reacted with chitosan's amino group but did not react with the hydroxyl group. Removal of various alkylphenols performed in this study was also based on the same reaction mechanism.
In addition, it can be seen from Figures 1-3 that higher conversion percent values were obtained in the presence of chitosan beads than in the absence of chitosan beads. The ratio of the conversion percent value in the presence of chitosan beads to that in the absence of chitosan beads was calculated and defined as the initial velocity ratio. Figure 4 shows changes in the initial velocity ratio with the PPO concentration for the linear alkylphenols used. The initial velocity ratio decreased with an increase in PPO concentration. Inhibition of enzymatic activity was found to be suppressed through a decrease  Next, Figure 5 shows changes in the initial velocity ratio with the PPO concentration for the branched alkylphenols used. The initial velocity ratio for branched alkylphenols gradually decreased with an increase in enzyme concentration. As shown in Figure 4, the initial velocity ratio for the linear alkylphenols ranged from 1.1 to 2.6, whereas those for the branched alkylphenols ranged from 2.4 to 4.4. This indicated that the enzymatic activity of PPO apparently increased by dispersing chitosan beads in the alkylphenol solutions, and in particular, this behavior was more prominently observed for the branched alkylphenols. Inhibition of enzyme activity was found to be more highly suppressed by quinone adsorption on the chitosan beads for the branched alkylphenols. Chitosan beads were dispersed in alkylphenol solutions without PPO at pH 7.0 and 40 °C, and then the solutions were constantly stirred. Figure 6 shows the amount of alkylphenols physically or electrostatically adsorbed on the chitosan beads after stirring for 6 and 24 h. The adsorbed amount increased with an increase in alkyl chain length of the alkylphenols used and/or the stirring time. However, a considerable amount of alkylphenols were left in the solutions. This result emphasized that although PPO dose was required to be adjusted, depending on the length and branching of alkyl chains, the above-mentioned two-step procedure was quite effective in removing linear and branched alkylphenols from the aqueous medium. Next, Figure 5 shows changes in the initial velocity ratio with the PPO concentration for the branched alkylphenols used. The initial velocity ratio for branched alkylphenols gradually decreased with an increase in enzyme concentration. As shown in Figure 4, the initial velocity ratio for the linear alkylphenols ranged from 1.1 to 2.6, whereas those for the branched alkylphenols ranged from 2.4 to 4.4. This indicated that the enzymatic activity of PPO apparently increased by dispersing chitosan beads in the alkylphenol solutions, and in particular, this behavior was more prominently observed for the branched alkylphenols. Inhibition of enzyme activity was found to be more highly suppressed by quinone adsorption on the chitosan beads for the branched alkylphenols.  Next, Figure 5 shows changes in the initial velocity ratio with the PPO concentration for the branched alkylphenols used. The initial velocity ratio for branched alkylphenols gradually decreased with an increase in enzyme concentration. As shown in Figure 4, the initial velocity ratio for the linear alkylphenols ranged from 1.1 to 2.6, whereas those for the branched alkylphenols ranged from 2.4 to 4.4. This indicated that the enzymatic activity of PPO apparently increased by dispersing chitosan beads in the alkylphenol solutions, and in particular, this behavior was more prominently observed for the branched alkylphenols. Inhibition of enzyme activity was found to be more highly suppressed by quinone adsorption on the chitosan beads for the branched alkylphenols. Chitosan beads were dispersed in alkylphenol solutions without PPO at pH 7.0 and 40 °C, and then the solutions were constantly stirred. Figure 6 shows the amount of alkylphenols physically or electrostatically adsorbed on the chitosan beads after stirring for 6 and 24 h. The adsorbed amount increased with an increase in alkyl chain length of the alkylphenols used and/or the stirring time. However, a considerable amount of alkylphenols were left in the solutions. This result emphasized that although PPO dose was required to be adjusted, depending on the length and branching of alkyl chains, the above-mentioned two-step procedure was quite effective in removing linear and branched alkylphenols from the aqueous medium. Chitosan beads were dispersed in alkylphenol solutions without PPO at pH 7.0 and 40 • C, and then the solutions were constantly stirred. Figure 6 shows the amount of alkylphenols physically or electrostatically adsorbed on the chitosan beads after stirring for 6 and 24 h. The adsorbed amount increased with an increase in alkyl chain length of the alkylphenols used and/or the stirring time. However, a considerable amount of alkylphenols were left in the solutions. This result emphasized that although PPO dose was required to be adjusted, depending on the length and branching of alkyl chains, the above-mentioned two-step procedure was quite effective in removing linear and branched alkylphenols from the aqueous medium.

PPO-Catalyzed Quinone Oxidation and Subsequent Quinone Adsorption on Chitosan Powder
Since the alkylphenols used were removed through quinone adsorption on chitosan beads, the removal of 4NBP, 4SBP, 4TBP, 4NPenP, and 4TPenP by the above-mentioned two-step procedure was investigated with three kinds of chitosan powders in place of chitosan beads. Figure 7 shows the time course in the absorbance and conversion percent value for 4NPenP solutions containing PPO, in which the three kinds of chitosan powders or chitosan beads were dispersed. 4TPenP was also successfully removed through quinone adsorption on chitosan powders. As the diameter of the chitosan powders used was lower, the conversion percent value increased at shorter reaction times. Such behavior was also observed for 4NBP, 4SBP, 4TBP, and 4TPenP (not shown). The minimum absorbances and conversion percent values were summarized in Table 3 in the case where PPO was added to these five kinds of alkylphenol solutions in the presence of chitosan powders or chitosan beads at pH 7.0 and 40 °C. On the supposition that the chitosan powders used were perfect spheres with an average diameter of 26, 58, and 87 μm, and chitosan had a density of 1.0 g/cm 3 in the dry state, the specific surface areas of the chitosan powders with the diameters of 10-42, 42-74, and 74-100 μm were calculated to be 0.231, 0.103, and 0.069 m 2 /g, respectively [22]. These values were much smaller than the nominal specific surface area value (70-100 m 2 /g) of the chitosan beads used. These five kinds of alkylphenols were highly removed by quinone adsorption on chitosan powders with diameters of 10-42 and 42-74 μm. It was found from these results that if the chitosan powders were used in the heterogeneous system at pH 7.0, these alkylphenols were more effectively removed than the chitosan beads. In other words, it was especially important that enzymatically generated quinone derivatives reacted with chitosan in the heterogeneous system. However, it was quite difficult to determine the amount of amino groups present on the surfaces of the chitosan powders, because chitosan was insoluble at pH 7.0.

PPO-Catalyzed Quinone Oxidation and Subsequent Quinone Adsorption on Chitosan Powder
Since the alkylphenols used were removed through quinone adsorption on chitosan beads, the removal of 4NBP, 4SBP, 4TBP, 4NPenP, and 4TPenP by the above-mentioned two-step procedure was investigated with three kinds of chitosan powders in place of chitosan beads. Figure 7 shows the time course in the absorbance and conversion percent value for 4NPenP solutions containing PPO, in which the three kinds of chitosan powders or chitosan beads were dispersed. 4TPenP was also successfully removed through quinone adsorption on chitosan powders. As the diameter of the chitosan powders used was lower, the conversion percent value increased at shorter reaction times. Such behavior was also observed for 4NBP, 4SBP, 4TBP, and 4TPenP (not shown). The minimum absorbances and conversion percent values were summarized in Table 3 in the case where PPO was added to these five kinds of alkylphenol solutions in the presence of chitosan powders or chitosan beads at pH 7.0 and 40 • C. On the supposition that the chitosan powders used were perfect spheres with an average diameter of 26, 58, and 87 µm, and chitosan had a density of 1.0 g/cm 3 in the dry state, the specific surface areas of the chitosan powders with the diameters of 10-42, 42-74, and 74-100 µm were calculated to be 0.231, 0.103, and 0.069 m 2 /g, respectively [22]. These values were much smaller than the nominal specific surface area value (70-100 m 2 /g) of the chitosan beads used. These five kinds of alkylphenols were highly removed by quinone adsorption on chitosan powders with diameters of 10-42 and 42-74 µm. It was found from these results that if the chitosan powders were used in the heterogeneous system at pH 7.0, these alkylphenols were more effectively removed than the chitosan beads. In other words, it was especially important that enzymatically generated quinone derivatives reacted with chitosan in the heterogeneous system. However, it was quite difficult to determine the amount of amino groups present on the surfaces of the chitosan powders, because chitosan was insoluble at pH 7.0.

PPO-Catalyzed Quinone Oxidation and Subsequent Quinone Adsorption on Chitosan Powder
Since the alkylphenols used were removed through quinone adsorption on chitosan beads, the removal of 4NBP, 4SBP, 4TBP, 4NPenP, and 4TPenP by the above-mentioned two-step procedure was investigated with three kinds of chitosan powders in place of chitosan beads. Figure 7 shows the time course in the absorbance and conversion percent value for 4NPenP solutions containing PPO, in which the three kinds of chitosan powders or chitosan beads were dispersed. 4TPenP was also successfully removed through quinone adsorption on chitosan powders. As the diameter of the chitosan powders used was lower, the conversion percent value increased at shorter reaction times. Such behavior was also observed for 4NBP, 4SBP, 4TBP, and 4TPenP (not shown). The minimum absorbances and conversion percent values were summarized in Table 3 in the case where PPO was added to these five kinds of alkylphenol solutions in the presence of chitosan powders or chitosan beads at pH 7.0 and 40 °C. On the supposition that the chitosan powders used were perfect spheres with an average diameter of 26, 58, and 87 μm, and chitosan had a density of 1.0 g/cm 3 in the dry state, the specific surface areas of the chitosan powders with the diameters of 10-42, 42-74, and 74-100 μm were calculated to be 0.231, 0.103, and 0.069 m 2 /g, respectively [22]. These values were much smaller than the nominal specific surface area value (70-100 m 2 /g) of the chitosan beads used. These five kinds of alkylphenols were highly removed by quinone adsorption on chitosan powders with diameters of 10-42 and 42-74 μm. It was found from these results that if the chitosan powders were used in the heterogeneous system at pH 7.0, these alkylphenols were more effectively removed than the chitosan beads. In other words, it was especially important that enzymatically generated quinone derivatives reacted with chitosan in the heterogeneous system. However, it was quite difficult to determine the amount of amino groups present on the surfaces of the chitosan powders, because chitosan was insoluble at pH 7.0.

Removal with PPO and Chitosan in the Homogeneous System
PPO was added to the solutions of 4NBP, 4SBP, 4TBP, 4NPenP, and 4TPenP containing chitosan at different amino group concentrations in a pH 6.0 buffer at 40 • C. The reason for the use of a pH 6.0 buffer was simply because chitosan was insoluble at pH values higher than 6.0, and the degree of protonation was minimized. Figure 8 shows changes in the absorbance with the amino group concentration or the molar ratio of chitosan amino groups to alkylphenols. When enzymatically generated quinone derivatives reacted with amino groups of chitosan [20,22,25,26], water-insoluble dark-brownish aggregates were generated. Absorbance decreased with an increase in amino group concentration to reach the minimum values. However, when the amino group concentration was further increased, the absorbance sharply increased for 4NBP, 4SBP, and 4NPenP and gradually increased for 4TBP and 4TPenP.
At low amino group concentrations, free quinone derivatives were left in the solution, and aggregate scarcely formed because chitosan was in short supply. On the other hand, since many free amino groups were left on a chitosan chain, and some chitosan chains remained soluble in the solution at higher amino group concentrations, a high absorbance was empirically observed for 4NBP, 4SBP, and 4NPenP. The amino group concentrations at which minimum absorbance was observed were determined as the optimum value, and at these amino group concentrations, the time course of absorbance was measured. Figure 9 shows the time course of absorbance at amino group concentrations for 4NBP, 4SBP, 4TBP, 4NPenP, and 4TPenP. The used amino group concentration was 0.225 mM for 4NBP, 4SBP, and 4NPenP and 0.45 mM for 4TBP and 4TPenP. Absorbance increased by the generation of quinone derivatives at a short reaction time depending on the length and branching of the alkyl chain. After minimum absorbance, the absorbance sharply decreased for 4NBP and 4NPenP and gradually decreased for 4SBP, 4TBP, and 4TPenP against the reaction time by the generation of aggregates. The sharp decrease in the absorbance suggested that the quinone derivatives enzymatically generated from 4NBP and 4NPenP successfully reacted with chitosan's amino groups. On the other hand, the reactions of quinone derivatives generated from 4SBP, 4TBP, and 4TPenP with chitosan's amino groups were considered to be considerably slow. In addition, another reason was that quinone derivatives generated from 4TBP and 4TPenP were less stable in the aqueous medium [27]. As shown in Table 3, these five kinds of alkylphenols were almost completely removed for 3 to 5 h by quinone oxidation and subsequent quinone adsorption on chitosan beads, whereas in the homogeneous reaction, formation of aggregates was not observed at theses reaction times (Figure 9). Consequently, this comparison emphasizes that alkylphenols can be removed in much shorter reaction times for the heterogeneous two-step reaction with PPO and chitosan beads than for the homogeneous reaction.

Removal with PPO and Chitosan in the Homogeneous System
PPO was added to the solutions of 4NBP, 4SBP, 4TBP, 4NPenP, and 4TPenP containing chitosan at different amino group concentrations in a pH 6.0 buffer at 40 °C. The reason for the use of a pH 6.0 buffer was simply because chitosan was insoluble at pH values higher than 6.0, and the degree of protonation was minimized. Figure 8 shows changes in the absorbance with the amino group concentration or the molar ratio of chitosan amino groups to alkylphenols. When enzymatically generated quinone derivatives reacted with amino groups of chitosan [20,22,25,26], water-insoluble dark-brownish aggregates were generated. Absorbance decreased with an increase in amino group concentration to reach the minimum values. However, when the amino group concentration was further increased, the absorbance sharply increased for 4NBP, 4SBP, and 4NPenP and gradually increased for 4TBP and 4TPenP.  At low amino group concentrations, free quinone derivatives were left in the solution, and aggregate scarcely formed because chitosan was in short supply. On the other hand, since many free amino groups were left on a chitosan chain, and some chitosan chains remained soluble in the solution at higher amino group concentrations, a high absorbance was empirically observed for 4NBP, 4SBP, and 4NPenP. The amino group concentrations at which minimum absorbance was observed were determined as the optimum value, and at these amino group concentrations, the time course of absorbance was measured. Figure 9 shows the time course of absorbance at amino group concentrations for 4NBP, 4SBP, 4TBP, 4NPenP, and 4TPenP. The used amino group concentration was 0.225 mM for 4NBP, 4SBP, and 4NPenP and 0.45 mM for 4TBP and 4TPenP. Absorbance increased by the generation of quinone derivatives at a short reaction time depending on the length and branching of the alkyl chain. After minimum absorbance, the absorbance sharply decreased for 4NBP and 4NPenP and gradually decreased for 4SBP, 4TBP, and 4TPenP against the reaction time by the generation of aggregates. The sharp decrease in the absorbance suggested that the quinone derivatives enzymatically generated from 4NBP and 4NPenP successfully reacted with chitosan's amino groups. On the other hand, the reactions of quinone derivatives generated from 4SBP, 4TBP, and 4TPenP with chitosan's amino groups were considered to be considerably slow. In addition, another reason was that quinone derivatives generated from 4TBP and 4TPenP were less stable in the aqueous medium [27]. As shown in Table 3, these five kinds of alkylphenols were almost completely removed for 3 to 5 h by quinone oxidation and subsequent quinone adsorption on chitosan beads, whereas in the homogeneous reaction, formation of aggregates was not observed at theses reaction times ( Figure 9). Consequently, this comparison emphasizes that alkylphenols can be removed in much shorter reaction times for the heterogeneous two-step reaction with PPO and chitosan beads than for the homogeneous reaction.

Conclusion
In this study, removal of linear and branched alkylphenols was investigated with the combined use of PPO-catalyzed quinone oxidation and subsequent reaction of enzymatically generated quinone derivatives with chitosan in different forms of porous beads, powders, and solutions. At pH 7.0 and 40 °C, the alkylphenols used were successfully converted into the corresponding quinone derivatives by PPO. Quinone oxidation occurred at lower PPO concentrations for alkylphenols with shorter alkyl chains.

Conclusions
In this study, removal of linear and branched alkylphenols was investigated with the combined use of PPO-catalyzed quinone oxidation and subsequent reaction of enzymatically generated quinone derivatives with chitosan in different forms of porous beads, powders, and solutions. At pH 7.0 and 40 • C, the alkylphenols used were successfully converted into the corresponding quinone derivatives by PPO. Quinone oxidation occurred at lower PPO concentrations for alkylphenols with shorter alkyl chains.
When PPO was added to alkylphenol solutions in the presence of chitosan beads, the alkylphenols were removed by chemical adsorption, or chemisorption, of enzymatically generated quinone derivatives on chitosan beads, and the removal time was shortened by increasing PPO concentration. Here, the activity of PPO apparently increased as a result of the decrease in quinone concentration by adsorption on chitosan beads. This behavior is quite favorable for this removal system. PPO catalyzed quinone oxidation of alkylphenols without H 2 O 2 at lower concentrations compared to mushroom tyrosinase, although a higher PPO dose was required for quinone oxidation of branched alkylphenols than for that of the corresponding linear ones. Consequently, it should be noted that 4SBP, 4TBP, 4TPenP, and 4TOP, suspected as endocrine-disrupting chemicals, were also successfully removed for 1.5-4 h. Even if chitosan powders were dispersed in place of chitosan beads in the alkylphenol solution containing PPO at pH 7.0, alkylphenols were removed by quinone adsorption, and removal time was shortened as the diameter of the chitosan powder decreased.
On the other hand, in the homogeneous system with solutions of PPO and chitosan at pH 6.0, the alkylphenols used were removed through the formation of aggregates by the chemical reaction of enzymatically generated quinone oxidation with chitosan's amino groups. However, much longer removal times were required compared to heterogeneous systems with chitosan beads or powders. These results emphasize the fact that quinone adsorption in the heterogeneous system is quite effective in removing alkylphenols from the aqueous medium. In addition, we can safely say that an alternative use of chitosan, which is produced in large amounts by deacetylation of chitin present in the shells of crustaceans such as crabs and prawns, can be found out in this study. From now on, we will perform immobilization of PPO on an insoluble solid carrier to investigate removal of alkylphenols by immobilized PPO and repetitive use of immobilized PPO.