New Alginate/PNIPAAm Matrices for Drug Delivery

This paper deals with a comparative study on the interpolymeric complexes of alginate poly(N-isopropyl acryl amide (PNIPAAm) and corresponding graft copolymers with various compositions in respect to their toxicity, biocompatibility and in vitro and in vivo release of theophylline (THP). Loading of the various matrices with theophylline and characterization of loaded matrices was studied by near infrared spectroscopy–chemical imaging (NIR–CI) analysis, scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). It was appreciated that THP loading is higher than 40% and the drug is relatively homogeneous distributed within all matrices because of some specific interactions between components of the system. All samples have been found to be non-toxic and biocompatible. It was established that graft copolymers having a good stability show a better drug carrier ability, a higher THP loading, a prolonged release (longer release duration for graft copolymers of 235.4–302.3 min than that for IPC 72/28 of 77.6 min, which means approximately four times slower release from the graft copolymer-based matrices than from the interpolymeric complex) and a good bioavailability. The highest values for THP loading (45%), prolonged release (302.3 min) and bioavailability (175%) were obtained for graft copolymer AgA-g-PNIPAAm 68. The drug release mechanism varies with composition and architecture of the matrix.


Introduction
In the past decade the development of industry and industrialized activities have generated not only concerns about environmental pollution but also about people's health [1]. This was reflected in the increase of the cases of people from all age categories showing health problems due to environmental contamination, especially oral mucosal and respiratory diseases. Generally, for alleviation and treatment of these diseases, oral, parenteral or pulmonary delivery methods are the most frequently applied. Generally, when a route administration is selected, for a very efficient therapeutic effect a lower administered dose and reduced side effects should be accomplished [1][2][3].
A great challenge among the currently used drugs for these purposes is the burst release of the therapeutical agent from the formulations which could lead to side effects concerning an inefficient therapy with further effects at the tissue level and in blood concentrations. To obtain a controlled, prolonged and targeting drug release, various kinds of matrices have been proposed as: electrospun polymer matrices, electrospun polymer patches, and nanofibres [4,5], hydro (gel) microspheres and nanogels [6][7][8], drug-conjugated thermogels [9], etc.
The development of delivery systems has accelerated in the past decade, as new and modern synthesis and characterization methods were implemented. Polymers were reported as being 1.
The content in PNIPAAm found varied from 30% to 68% and average viscosity molecular weight (M v ) of the PNIPAAm side chains were about 19-35 kDa.
Both the interpolymeric complex AgA/PNIPAAm IPC (IPC 72/28) and the graft copolymers, mainly AgA-g-PNIPAAm 43 (C43) and AgA-g-PNIPAAm 68 (C68), were selected for biocompatibility study and in vitro/in vivo release behavior of theophylline (THP) (the numbers represent the PNIPAAm content in graft copolymers). Both groups of studied samples are dual-responsive materials showing sudden changes at a certain range of both pH and temperature variation, and therefore their application as matrices for drug delivery is interesting for obtaining prolonged and targeted release.
The final end-products were processed up for the applied tests: i. Suspensions in water and carboxymethyl cellulose (5 wt %) for the in vivo drug release. ii. Lyophilized form for the physical chemical characterization.
Anhydrous crystalline theophylline supplied by BASF (Copenhagen, Denmark) was chosen as model drug for the release experiments, purity 97 to 100 wt %.

Preparation of Theophylline-Loaded Samples
The loading of theophylline within the polymeric matrices (IPC and graft copolymers) was done by mixing the drug with the lyophilized powdered matrices and using as solvent distilled water obtaining a semi-solid formulation. The concentration of theophylline in the polymeric matrix was 5 wt %. The suspensions obtained were freeze-dried using a Labconco FreeZone device (Kansas City, MO, USA), in order to obtain lyophilized sponge-like materials or powders to be further used for the in vitro measurements.
For the in vivo experiments the drug loading was performed in a similar manner by forming a semi-solid formulation in 1/1 ethanol/water mixture which swells better the polymer network and the solubility of the drug was higher.
The loading of formed hydrogels was done by adsorption of theophylline as a concentrated solution of 4 wt % (1/1, v/v, ethyl alcohol/ water). The mixture was mildly stirred for 24 h, to favor the polymeric system to reach the equilibrium swelling degree. The efficiency of the polymeric matrices to load theophylline was tested by means of near infrared (NIR) spectroscopy and high-performance liquid chromatography (HPLC) studies.

Investigation Methods
The polymeric matrices (IPC and graft copolymers) based on alginate and PNIPAAm and theophylline-loaded matrices were tested for their toxicity and biocompatibility. Additionally, the matrices were characterized on their particle size, thermal behavior, distribution of the drug within the polymeric matrix, morphology, and in vitro and in vivo drug-delivery profiles.

Near Infrared Spectroscopy-Chemical Imaging (NIR-CI)
Near infrared spectroscopy-chemical imaging (NIR-CI) was used to determine the distribution of the theophylline into polymeric matrices and the chemical homogeneity of the unloaded and drug loaded matrices. Samples as powder were measured by means of NIR spectra on a SPECIM'S Ltd. Sisu CHEMA instrument (Spectral Imaging Ltd. Oulu, Finland) controlled with the Evince software package for processing the original image data. The system used was equipped with a Chemical Imaging Workstation for 1000-2500 nm NIR domains. The original image for each sample was taken with a NIR model spectral camera, respectively an imaging spectrograph type ImSpector N17E (Specim, Spectral Imaging Ltd. Oulu, Finland (with 320 and 640 pixel spatial resolution at a rate of 60-350 Hz.

Scanning Electron Microscopy (SEM)
Microscopic investigations of the unloaded and loaded hydrogels as dried sponges with theophylline were performed. Analyzed samples were firstly frozen by direct immersion in liquid nitrogen and then fractured. After metallization with gold, the sample examination was performed on a Quanta 3D scanning electron microscope (SEM, Thermo Fisher Scientific, Waltham, MA, USA). Magnification is given on the images.

Thermogravimetric Analysis (TGA)
The thermo-oxidative behavior of the IPC and grafted copolymers of AgA/PNIPAAm loaded with theophylline was evaluated by thermogravimetric analysis (TGA) using a Q 500 (TA Instruments, New Castel, DE, USA) thermal analyzer. For each recording, 5 mg of sample was placed in Al 2 O 3 crucibles. The crucibles were placed in a small electrically heated oven with a thermocouple to accurately measure the temperature ensuring high precision in weight, temperature, and temperature change. Temperature calibration was done with standard indium, zinc, tin, bismuth, and aluminum of 99.99% purity. The thermoravimetry was performed in an air/autogenerated atmosphere, at a heating rate of 20 • C/min, in the 15-700 • C temperature range. The characteristic temperatures determination from derivative TGA (DTGA) curves has a reproducibility of ±2 • C.

Ethics Statement for Experiments with Animals
The experiments on animals were performed according to the institutional guidelines for care and use of laboratory animals and the experimental research protocol was approved by the Animal Research Ethics Committee of "Grigore T. Popa" University of Medicine and Pharmacy of Iasi, Romania (official paper no. 15559/21.09.2010) in rigorous accordance with international ethical regulations on laboratory animal work.
The laboratory animals were anesthetized by exposure to diethyl ether vapors via the respiratory route by exposing them to ether overdose for approximately 2 min in transparent acrylic closed containers. The experiments were performed in compliance with safety regulations [29,30]. Groups of six animals were used for each kind of experiment.

Toxicity and Biocompatibility Studies
Biocompatibility of these systems was tested within the Laboratories of "Grigore T. Popa" Medicine and Pharmacy University, Iasi, Romania. The toxicity and specific biocompatibility tests were performed on white Swiss strain male mice (25-30 g) by determining the rate of living of white Swiss mice after intra-peritoneal injection of the suspensions of polymeric matrices loaded with THP in carboxymethyl cellulose 0.5 wt %. Specific parameters like haemogram, the phagocytic capacity of neutrophils (NBT test), the opsonic capacity of serum, the phagocytic and bactericidal capacities of peritoneal macrophages and the hepatotoxic effects by determining the enzymatic levels of aspartate aminotransferase (AST), serum alanine aminotransferase (ALT) and lactate dehydrogenase (LD) were determined. Details on the methodology used for performing these tests were reported in previous similar studies applied on different matrices [23,31,32].

Statistical Analysis
The triplicate tests were performed and the obtained results are expressed as means ± SD (standard deviation) and significance was analyzed using the T-student test in Microsoft Excel for Windows.
The Stat View statistical software package (Apple Macintosh, Brain Power Inc., Cary, CA, USA) was used for data analysis. Analysis of variance (ANOVA) and Fisher's post hoc test consisting of 3 (groups), 3 (time sample points) repeated measures of experimental results were analyzed. The criterion for significance was p < 0.05.

In Vitro Theophylline Release
The in vitro release studies for theophylline have been performed by a standard dissolution test carried out in conditions which mimic the gastrointestinal environment, using acidic buffer solution of pH 2.2 as dissolution medium. During the experiment, the temperature was maintained at 37 ± 0.5 • C. Aliquots of the medium of 1 mL were withdrawn periodically at predetermined time intervals and analyzed using a HP 8450A ultraviolet (UV)-Visible spectrophotometer (Hewlett Packard, Palo Alto, CA, USA). Recordings were made at λ = 271 nm the wavelength characteristic to theophylline. In order to maintain constant the measured drug concentration, the sample was carefully reintroduced in the circuit after analysis.
The concentrations of the drugs were calculated based on calibration curves predetermined for the drug at specific maximum absorption wavelength mentioned above.
The drug release kinetics, Korsmeyer-Peppas semi-empirical Equation (1) was applied for the initial release stages (~60% fractional release) [33]: where M t /M ∞ is the fractional drug released, M t and M ∞ are the cumulative drug released amounts at time t and at equilibrium, respectively (or experimental maximum released amount taken at the plateau of the release curves), k r is rate constant dependent on the characteristics of the drug loaded system, and n r is the diffusional exponent which defines the type of the release mechanism. For example, a value of n r~0 .5 is characteristic of the Fickian diffusion mechanism of the drug from the cryogel; the values in interval 0.5 < n r < 1 are specific to an anomalous or non-Fickian behavior. A case II of the transport mechanism occurs when n r = 1, which means zero-order kinetics, while a special case II of the transport mechanism is indicated by values n r > 1 [34] The THP release profiles are plotted as the cumulative percentage of drug released versus time.

In Vivo Theophylline Release
The in vivo release of raw theophylline and theophylline loaded within the two types of matrices with alginate (IPC 72/28 and C68) were studied. Selection of loaded matrices for in vivo testing was firstly done based on the outcomes obtained from previous tests, more particularly in vitro tests and loading capacity determined by NIR analysis. In vitro release profiles of the three graft copolymers of alginate with PNIPAAm were almost similar. From the three graft copolymers, C25, C43 and C68, the C68 one with higher amount of PNIPAAm showed better capacity to load THP, and therefore it was selected to be in vivo tested.
In vivo release behavior of THP was investigated on adult Wistar rats and drug serum concentration was determined by HPLC assay. An HPLC (Shimadzu Model-CTO-20A HPLC system, Osaka, Japan) was used. The separation was performed on a 5 µm ZORBAX SB-C18 column (150 mm × 4.6 mm i.d.) in the following conditions: mobile phase composed of acetonitrile10 mM aqueous sodium acetate (7:93 v/v) under a flow rate of 1 mL/min at room temperature, injection volume of 100 µL; THP was detected by UV detector at 270 nm. A calibration curve with standard solutions of known THP concentrations (1-20 µg/mL) in ethyl alcohol/water (1:1 v/v) was drawn. The software LC Solution Version 1.22 SP1 was used for integration and automatic determination of drug concentration in blood samples. Other details on experiments and on the pharmacokinetics and in vivo release studies were performed similarly as previously reported for other polymeric matrices [23,31,32].
Oral delivery was chosen as it is a common method of drug administration. The animals were maintained in identical laboratory conditions as before, with free access to food and water. The raw theophylline and theophylline-loaded matrices were delivered by gastro-gavage as suspensions, in a dose of 15 mg/kg body weight for each rat [35]. The in vivo release protocol and methodology were detailed as well in our previous studies [23,31,32]. The pharmacokinetic (PK) parameters such as maximum plasma concentration (C max ), time of maximum concentration (t max ) and plasma elimination half-life (t 1/2 ) were obtained directly from the plasma concentration-time plots. The area under the plasma concentration-time curve up to 72 h (AUC 0-72 ) was calculated using the linear trapezoidal rule. The relative bioavailability (test/reference ratios) of the chitosan based hydrogel formulations, compared to raw theophylline was calculated as the ratio (AUC sample /AUC theophylline ) × 100. Each experiment was repeated four times and the results were represented by means of standard deviation.

Naer Infrared (NIR) Results
Theophylline distribution into AgA/PNIPAAm systems (IPC and graft copolymers) was evaluated by the NIR-CI technique. The chemical imaging provides a simple method for evaluating the spatial drug distribution. The drug-loading degree was evaluated based the near infrared chemical imaging maps. The non-destructive character and the facile way to prepare the samples promoted this technique as very feasible to investigate materials for pharmaceutical applications. The homogeneity and prediction of the two constituents of the system (i.e., drug and the polymeric matrix) were assessed by applying two mathematical models, particularly, PLS-DA (partial least squares-discriminate analysis) and PCA (principal component analysis). The PLS-DA model based on the multivariate inverse least squares discrimination method is used to classify the components by Evince software. To each constituent a color was assigned, and applying the aforementioned model the assessment of the degree of homogeneity of the components was undertaken. A value between 0 and 1 of the same original component was assigned and facilitated to obtain quantitative information. For the first component, value 1 represents available percentage of 100% and the value tends to zero when there is an unknown structure. It is appreciated the correct information available upon the cube of information can be extracted. The final images have for every pixel a complete spectrum that includes contributions from all the chemical components present in system.
The images in Figure 1 correspond to the PLS-DA model for IPC 78/28 and two copolymers AgA-g-PNIPAAm C43 and C68. A visibly uniform distribution, corresponding to a high homogeneity degree of the drug in the matrices was observed. Based on the PLS-DA prediction, a drug loading up to 60% into the IPC and 45% into AgA-g-PNIPAAm copolymers was found from the theophylline-loaded amount- Table 1. The non-destructive character and the facile way to prepare the samples promoted this technique as very feasible to investigate materials for pharmaceutical applications. The homogeneity and prediction of the two constituents of the system (i.e. drug and the polymeric matrix) were assessed by applying two mathematical models, particularly, PLS-DA (partial least squares-discriminate analysis) and PCA (principal component analysis). The PLS-DA model based on the multivariate inverse least squares discrimination method is used to classify the components by Evince software. To each constituent a color was assigned, and applying the aforementioned model the assessment of the degree of homogeneity of the components was undertaken. A value between 0 and 1 of the same original component was assigned and facilitated to obtain quantitative information. For the first component, value 1 represents available percentage of 100% and the value tends to zero when there is an unknown structure. It is appreciated the correct information available upon the cube of information can be extracted. The final images have for every pixel a complete spectrum that includes contributions from all the chemical components present in system.
The images in Figure 1 correspond to the PLS-DA model for IPC 78/28 and two copolymers AgA-g-PNIPAAm C43 and C68. A visibly uniform distribution, corresponding to a high homogeneity degree of the drug in the matrices was observed. Based on the PLS-DA prediction, a drug loading up to 60% into the IPC and 45% into AgA-g-PNIPAAm copolymers was found from the theophylline-loaded amount- Table 1.    According to the data obtained, a quantity of 1.53-1.8% unknown compound is detected which indicate some interactions between components of the systems. In Figure 2a   According to the data obtained, a quantity of 1.53-1.8% unknown compound is detected which indicate some interactions between components of the systems. In Figures 2a,b are shown the NIR spectra of theophylline, for IPC 72/28 ( Figure 2a) and AgA-g-PNIPAAm, C43 and C68 (Figure 2b) unloaded and loaded with theophylline in the full range of the near infrared region.  The most visible differences between the loaded and unloaded matrices were observed in the 2095 and 2140 cm −1 spectral region which were assigned to the O-H, N-H combinations' stretching vibrations, confirming the presence of the drug within the matrices and its interaction with the polymeric moieties via H-bonds. Additional information regarding the assignments of the bands is included in Table 2. The most characteristic bands of THP are distinctly identified in loaded matrices or they are shifted to the lower wavenumber indicated as already mentioned, the interactions between components in all three cases- Table 2. In particular, theophylline showed more visible specific bands at about 2475 nm corresponding to the C-N-C bond.
The bands corresponding to the functional groups of IPC and the copolymers are mostly overlapped with those ones of theophylline. C-H and C-C combinations C-N-C stretch overtone

Scanning Electron Microscope (SEM) Images
Polymeric matrices, both IPC and graft copolymers had a porous structure due to the lyophilization. The SEM images (Figure 3a-d) confirmed that theophylline as a crystalline drug was dispersed within the polymeric matrices as stick-shaped like microparticles. The various dimensions of the theophylline particles dispersed between the polymeric matrices were determined and they were in the range of 10 to 50 µm.
SEM images confirmed the NIR-CI results regarding the uniform distribution of theophylline within the polymer matrices.   SEM images confirmed the NIR-CI results regarding the uniform distribution of theophylline within the polymer matrices.

TGA Results
The thermal characteristics were studied by means of thermogravimetry. The thermogravimetric analysis was undertaken in order to evidence presence of drug in matrices and also the interactions taking place between the polymeric matrices and loaded drug. This information is obtained by studying of any physical and chemical processes occurring by the application of heat or during storage. Figure 4a-f shows the TGA and their derivative (DTGA) curves obtained for various alginate/PNIPAAm matrices unloaded and loaded with theophylline. Additionally, the thermal behavior on the degradation of pure alginate and THP were studied for a better understanding of the degradation of the more complex systems (IPC and graft copolymers).

TGA Results
The thermal characteristics were studied by means of thermogravimetry. The thermogravimetric analysis was undertaken in order to evidence presence of drug in matrices and also the interactions taking place between the polymeric matrices and loaded drug. This information is obtained by studying of any physical and chemical processes occurring by the application of heat or during storage. Figure 4a-f shows the TGA and their derivative (DTGA) curves obtained for various alginate/PNIPAAm matrices unloaded and loaded with theophylline. Additionally, the thermal behavior on the degradation of pure alginate and THP were studied for a better understanding of the degradation of the more complex systems (IPC and graft copolymers).
By comparative analysis of the TG/DTGA curves of theophylline loaded samples and pure polymer and drug, it was observed that weight loss of the loaded polymeric matrices occurred up to 700 • C in six overlapping steps which corresponded to the individually degradation of the components of the systems. Table 3 summarizes the main thermal characteristics determined from these TG/DTGA curves. Table 3. Maximum temperature from DTGA for THP-loaded IPC, graft copolymers and their components and THP.

Sample
Maximum Temperature ( • C)-from DTGA Step 1 Step 2 Step 3 Step 4 Step 5 Step  By comparative analysis of the TG/DTGA curves of theophylline loaded samples and pure polymer and drug, it was observed that weight loss of the loaded polymeric matrices occurred up to 700 °C in six overlapping steps which corresponded to the individually degradation of the components of the systems. Table 3 summarizes the main thermal characteristics determined from these TG/DTGA curves. The first step, occurring within the range 37-60 • C, could be assigned to the moisture loss which counts for about 8 to 11 wt %. The dehydration process is followed by several successive degradation steps, cumulating a mass loss of 65% to 80% corresponding to the main decomposition processes of alginate between 250 to 270 • C, drug entrapped which decomposes at about 290-340 • C, PNIPAAm decomposition at about 400 • C. As observed, the DTGA curves of the loaded samples showed a complex decomposition process between 250 and 540 • C, due to the drug-polymeric matrix interactions and polymer-polymer interactions. With the addition of THP within the polymeric matrices (IPC and graft copolymers), the thermal stability was increased in respect of that of alginate. Similar results for degradation of semi-IPN alginate/PNIPAAm were obtained, the outcome of improved thermal behavior of the drug loaded polymeric matrices being confirmed [23].
Because the peaks in DTG overlap, the weight loss corresponding to each step is not possible to evaluate correctly.

Toxicity Tests
The toxicity test supposed the intraperitoneal (i.p.) administration of a single dose of 2000 mg polymer suspension per kg body at a mouse, of each type of formulation [35]. Because the mice survived up to 2 weeks after administration, four other mice were injected for each composition, and their survival rate was assessed. It was found that all mice survived 14 days after i.p. administration of polymeric suspensions. No behavioral or physical changes such as abdominal swelling were observed in treated mice following injection or on subsequent days. Throughout the study period, animals showed no signs of peritonitis, lethargy, muscle loss, dehydration or anorexia, symptoms which are associated with animal toxicity [36]. The acute toxicity of a 5000 mg/kg [35] body dose was tested according to Organization for Economic Cooperation and Development (OECD) guidelines [37]. Due to technical problems occurred at administration of the solutions of such a high concentration, one single dose of 3200 mg kg body was tested. It was found that after a single dose of 3200 mg /kg body of polymeric suspension, intra-peritoneal administered to mice, for IPC 72/28 and AgA-g-PNIPAAm C43 and C68; they survived 14 days after administration.
The LD50 for IPC 72/28-THP and AgA-g-PNIPAAm C43 and C68-THP, after intraperitoneal administration of suspensions, was found to be higher than 3200 mg/kg. The obtained LD50 values demonstrated that the compositions have low toxicity.

Biocompatibility Studies
The in vivo biocompatibility of IPC 72/28 and C43 and C68 graft copolymers was examined during 14 consecutive days after i.p. injection of polymeric suspensions at mice. The effects on hematological and immune system parameters were followed comparatively with a control group of mice, which received simple physiological serum. The determined values of the hematological and immune systems parameters are listed within Table 4. Clinical chemistry and hematological data are of great importance to determine the effects induced on the body by the tested hydrogel matrices. The values of hematological parameters such as white blood cells (WBC), red blood cells (RBC), platelets (PLT), hemoglobin level (HGB) concentration, hematocrit level showed no significant variations between mice groups treated with IPC 72/28-THP and C-THP hydrogels compared with control mice group, being in the range of normal limits reported for healthy mice [38]. Additionally, the values obtained for these systems were comparable with similar matrices from alginate and PNIPAAm as semi-interpenetrated networks [23]. Statistical analysis revealed no significant influence of the studied compounds on the neutrophils phagocytic capacity, on the phagocytosis activity of immune cells, serum opsonic capacity, phagocytosis and bactericidal capacities of peritoneal macrophages, splenic lymphocytes with rosetting capacity of tested mice compared to the control group after 14 days of testing.
Based on the toxicity and biocompatibility results it can be considered that the IPC 72/28 and C68 graft copolymer based formulations are nontoxic and did not produce any significant changes in the hematology of tested rats and had no hepatic-toxicity effect. All presented results indicated a good biocompatibility of IPC 72/28 and C68 graft copolymer with living tissues, and so they could be considered as potential carriers for controlled drug delivery and other biomedical applications.

In Vitro Release of Theophylline
The release profiles of theophylline, as the dependence of the theophylline released as percent vs time, from the matrices of alginate and PNIPAAm with different architectures (IPC and graft copolymers) are shown in Figure 5. Based on the toxicity and biocompatibility results it can be considered that the IPC 72/28 and C68 graft copolymer based formulations are nontoxic and did not produce any significant changes in the hematology of tested rats and had no hepatic-toxicity effect. All presented results indicated a good biocompatibility of IPC 72/28 and C68 graft copolymer with living tissues, and so they could be considered as potential carriers for controlled drug delivery and other biomedical applications.

In Vitro Release of Theophylline
The release profiles of theophylline, as the dependence of the theophylline released as percent vs time, from the matrices of alginate and PNIPAAm with different architectures (IPC and graft copolymers) are shown in Figure 5. The THP released amount varied between 52.4% and 56.3% with small differences between samples, but they significantly differed in respect of the time to reach equilibrium (teq) which was much longer for graft copolymers of 235.4-302.3 min than that for IPC72/28 of 77.6 min. A burst The THP released amount varied between 52.4% and 56.3% with small differences between samples, but they significantly differed in respect of the time to reach equilibrium (t eq ) which was much longer for graft copolymers of 235.4-302.3 min than that for IPC72/28 of 77.6 min. A burst effect was characteristic of THP release from IPC, a large amount of drug being released in the first 25 min. The prolonged released in the case of graft copolymers was observed when amount of PNIPAAm graft copolymer composition was increased. A similar amount of theophylline was released almost four times slower than the graft copolymer-based matrices.
Drug release kinetics was assessed by applying the Korsmeyer-Peppas semi-empirical equation (Equation (1)) for the initial release stages (up to 60% fractional release). Values of release kinetic parameters were summarized within Table 5. The diffusional exponent n r takes value of 1.4 in the case of release of theophylline from IPC matrix and it varied from 0.7 to 1.8 in the case of graft copolymers and it increases with PNIPAAm content of copolymer. Gradual increase of diffusional exponent with the increase of the PNIPAAm content could indicate a different release mechanism. A special case II of transport mechanism is indicated by values n r > 1 which is characteristic of THP release from IPC72/28 and C68. Particularly, THP release from C25 occurs by an anomalous diffusion transport n r = 0.7 while a zero order kinetics (n = 1) is specific for the THP release from C43 copolymer which is known to be the most favorable method for drug delivery. Moreover, the increase of PNIPAAm content could lead to the enhanced sol-gel transition of the copolymer solution at 37 • C due to the temperature responsiveness induced by the PNIPAAm grafted chains. As a consequence, the diffusion of drug is limited by the gelation of the polymer matrix and, thus, has better resistance to erosion and less drug is released.
It can be concluded that it is possible to control the mechanism of THP release by changes in chemical composition of copolymers. The obtained results are supported also by those obtained in our previous studies where the influence of the physical chemical characteristics of the matrices was studied in much more detail [20][21][22].
As concerns the variation of the rate constant k r , which also takes characteristic values for each drug-loaded system, it can be observed that it took much lower values for the graft copolymers, which is 10 times slower from C68 than those for other copolymers and IPC 72/28. This could support the prolonged delivery of theophylline from the graft copolymers by comparison with the H-bonded interpolymeric complex based matrix, IPC 72/28.

In Vivo Theophylline Release
The in vivo release of raw theophylline and theophylline loaded within the two types of matrices with alginate (IPC 72/28 and C68) were studied. The mean plasma theophylline concentration versus time curves after a single oral dose are represented in Figure 6 and mean values of pharmacokinetic parameters (C max , t 1/2 , and AUC 0-72 ) are summarized in Table 6.
Pure theophylline was detected in plasma within the first hours after its administration in rats. The mean plasma level of raw theophylline was found of C max value of 7.1 µg/mL within a time period of t max of 1.5 h and the elimination half-life (t 1/2 ) of about 2.5 h, which indicated a fast absorption of pure theophylline, data that are consistent with previous studies [23]. Polymers 2019, 11, x FOR PEER REVIEW 16 of 19 Figure 6. In vivo release profiles of theophylline from IPC 72/28-THP and C68-THP.
As observed in Figure 6, there was a marked level when theophylline can have a toxic effect, particularly for a concentration of 20 g/mL [39][40][41][42]. Additionally, it was mentioned that the therapeutic drug level for theophylline is within the range 10-20 g/mL. A lower amount than 10 g/ml of delivered theophylline cannot ensure a complete therapeutic effect of the drug and a higher amount in blood plasma may induce a toxic effect. In the case of administration of free drug suspension, it could be observed that the detected concentration in sanguine plasma was up to 10 g/mL. When theophylline was carried by the polymeric vehicles (IPC and graft copolymer) the changes in the delivery profile were observed. In the case of IPC 72/28-THP, the delivery was prolonged by comparison with free theophylline delivery profile but with a rather low therapeutic level, and thus did not have enough of a therapeutic effect as would be needed. In the case of graft copolymer, theophylline was prolonged released and its concentration was ranged within the therapeutic level reported for theophylline without reaching a toxic level. The evaluated pharmacokinetic parameters are given in Table 6. The theophylline release profile showed higher values for the pharmacokinetic parameters in the case of the graft copolymers. This is translated by a retarded release of the drug, as the matrices were more complex and played their role in the drug protection against fast release. Higher bioavailability was also found, especially in the case of graft copolymer-based formulation C68. As observed in Figure 6, there was a marked level when theophylline can have a toxic effect, particularly for a concentration of 20 µg/mL [39][40][41][42]. Additionally, it was mentioned that the therapeutic drug level for theophylline is within the range 10-20 µg/mL. A lower amount than 10 µg/mL of delivered theophylline cannot ensure a complete therapeutic effect of the drug and a higher amount in blood plasma may induce a toxic effect. In the case of administration of free drug suspension, it could be observed that the detected concentration in sanguine plasma was up to 10 µg/mL. When theophylline was carried by the polymeric vehicles (IPC and graft copolymer) the changes in the delivery profile were observed. In the case of IPC 72/28-THP, the delivery was prolonged by comparison with free theophylline delivery profile but with a rather low therapeutic level, and thus did not have enough of a therapeutic effect as would be needed. In the case of graft copolymer, theophylline was prolonged released and its concentration was ranged within the therapeutic level reported for theophylline without reaching a toxic level. The evaluated pharmacokinetic parameters are given in Table 6. The theophylline release profile showed higher values for the pharmacokinetic parameters in the case of the graft copolymers. This is translated by a retarded release of the drug, as the matrices were more complex and played their role in the drug protection against fast release. Higher bioavailability was also found, especially in the case of graft copolymer-based formulation C68.

Conclusions
A comparative study on the interpolymeric complex and graft copolymers of AgA and PNIPAAm with various compositions in respect of their toxicity, biocompatibility and in vitro and in vivo release of theophylline was presented. THP-loaded and -unloaded polymeric carriers have been investigated in respect with their structure, morphology, thermal properties, toxicity and biocompatibility. All of them have been found to be non-toxic and biocompatible. It was established that graft copolymers of AgA-g-PNIPAAm exhibited better stability due to their structure and also showed a better drug carrier ability, a higher THP-loading ability, a prolonged release, and good bioavailability. The release mechanism varied with the composition and architecture of the matrix. Theophylline was released in a controlled manner and its concentration in sanguine plasma ranged within the therapeutic level reported for theophylline without reaching a toxic level.

Conflicts of Interest:
The authors declare no conflict of interest.