Structural Characterization of Lignin and Lignin-Carbohydrate Complex (LCC) from Ginkgo Shells (Ginkgo biloba L.) by Comprehensive NMR Spectroscopy

Lignin and lignin-carbohydrate complexes are important polymers for lignocellulosic biorefinery and functional materials, but those in ginkgo shells are not effectively analyzed and exploited. Based on this background, milled wood lignins (MWLML and MWLFZ) and lignin-carbohydrate complexes (LCCML and LCCFZ) were isolated from the shells of Ginkgo biloba L. cv. Damaling (ML) and Ginkgo biloba L. cv. Dafozhi (FZ) correspondingly, and were structurally characterized by comprehensive NMR spectroscopy. The results showed that ginkgo shells exhibited higher lignin (42%) and xylan (20%) content than general softwood species. Isolated MWLs were rich in guaiacyl units with the presence of ferulates and p-coumarates, and the molecular formula was C9H7.93O2.73(OCH3)0.81 and C9H7.87O2.76(OCH3)0.88 for MWLML and MWLFZ, respectively. Phenolic hydroxyl of MWLML (1.38 mmol/g) and MWLFZ (1.23 mmol/g) in ginkgo shells was much less than that in general softwoods, suggesting a higher etherification and condensation degree of ginkgo shells lignin, and β-5′, α-O-4′, and 4-O-5′ bonds were the main condensed structures. O-acetylated β-d-xylopyranoside and β-d-mannopyranoside were the main polysaccharides associated with lignin, and the acetyl groups frequently acylate the C2 and C3 positions. LCCML had more phenyl glycoside (0.035/Ar) and less γ-ester (0.026/Ar) linkages than LCCFZ.


Introduction
Lignocellulosic biomass is one of the economically viable and environmentally sustainable feedstocks for replacing fossil resources. It is mainly composed of three biopolymers: cellulose (40-60%), hemicelluloses (10-40%), and lignin (15-30%) [1]. Lignin is a complex macromolecule mainly synthesized from three p-hydroxycinnamyl alcohols (p-coumary, conifery, and sinaply) which give rise to p-hydroxyphenyl, guaiacyl, and syringyl units with a variety of interunit linkages (β-O-4 , α-O-4 , β-5 , β-1 , 5-5 , 4-O-5 , β-β , and so on). These structural units and linkages of lignin give plant cell walls many physicochemical properties and biological activities such as their rigidity and pathogen defense [2], and it is an important biopolymer for valorization [3,4]. For example, the phenolic groups in the lignin structure can be used to produce phenol formaldehyde resins [5]. Lignin with a low molecular weight and high quantity of phenolic hydroxyl groups positively contributes to the mechanical properties of a polyethylene-lignin composite [6]. Lignosulfonate, a water-soluble derivative of lignin, can utilize the redox chemistry of quinone to store energy [7].
use. The chemicals used in this work were all analytical or reagent grade and used as received without further purification.

Isolation of MWLs and LCCs
Milled wood lignin (MWL) was isolated according to the method described by Björkman [21], and the isolation procedure of lignin and LCC preparations from ginkgo shells is illustrated in Figure 1. The air-dried ginkgo shells were ground in a Wiley mill. Particles between 40 mesh (0.425 mm) and 80 mesh (0.180 mm) were extracted with ethanol/benzene (1:2, v/v) in a Soxhlet extractor for 12 h to obtain extractive-free samples. The extractive-free shell meals were milled in a planetary ball mill (Fritsch GMBH, Pulverisette 7 premium line, Idar-Oberstein, Germany) at a frequency of 10 Hz for 2 h without solvent. Two 80 mL silicon nitride bowls, with 4 g ginkgo shell meals in each bowl, were filled with 25 zirconium dioxide balls (1 cm diameter). The milling was conducted at room temperature, and 15 min intervals were provided between every 3 min of milling to prevent overheating.
The ball-milled samples were suspended in 1,4-dioxane (96%, v/v) with a liquid-to-solid ratio of 15 (mL/g) at room temperature for 24 h. The extraction procedure was conducted in the dark and under a nitrogen atmosphere. The mixture was centrifuged and washed using 96% 1,4-dioxane until the filtrate was clear. Such operations were repeated thrice. The extractive supernatants were combined and the solvent was recycled by vacuum evaporation. The crude lignin was dissolved in acetic acid (90%, w/w), and the soluble fraction was slowly introduced into deionized water. The supernatants were vacuum evaporated and washed to obtain LCCs (LCCML and LCCFZ). The precipitates were washed with deionized water to obtain lignin preparations (MWLML and MWLFZ). No further purification was performed for the preservation of the structural features of the lignin preparations.

Analytical Methods
Chemical components of ginkgo shells, MWLs, and LCCs were analyzed according to our previous work [22]. An elemental analyzer (2400 II, Waltham, MA, USA) was used to measure the content of carbon (C), hydrogen (H), nitrogen (N), and sulfur (S) elements of MWLs, and the amount of oxygen (O) element was calculated by the difference. The 13 C NMR of MWLs and 2D HSQC NMR of MWLs and LCCs were determined according to the method described by Huang et al. [23]. Acetylated MWLs were used for the determination of 1 H NMR spectra and molecular weight distribution [24]. Quantitative 31 P NMR was used for the determination of hydroxyl content in MWLs. A total of 40 mg of samples was dissolved in 500 µL of an anhydrous pyridine/CDCl3 mixture (1.6/1, v/v). Then,

Analytical Methods
Chemical components of ginkgo shells, MWLs, and LCCs were analyzed according to our previous work [22]. An elemental analyzer (2400 II, Waltham, MA, USA) was used to measure the content of carbon (C), hydrogen (H), nitrogen (N), and sulfur (S) elements of MWLs, and the amount of oxygen (O) element was calculated by the difference. The 13 C NMR of MWLs and 2D HSQC NMR of MWLs and LCCs were determined according to the method described by Huang et al. [23]. Acetylated MWLs were used for the determination of 1 H NMR spectra and molecular weight distribution [24]. Quantitative 31 P NMR was used for the determination of hydroxyl content in MWLs. A total of 40 mg of samples was dissolved in 500 µL of an anhydrous pyridine/CDCl 3 mixture (1.6/1, v/v). Then, 200 µL of an endo-N-hydroxy-5-norbornene-2,3-dicarboximide (9.23 mg/mL, internal Polymers 2018, 10, 736 4 of 17 standard) and 50 µL of chromium (III) acetylacetonate (5.6 mg/mL, relaxation reagent), prepared using the anhydrous pyridine/CDCl 3 , were added. The lignin solution was reacted with 100 µL of phosphitylating reagent (2-chloro-4,4,5,5-tetramethyl-1,2,3-dioxaphosphplane) for 15 min and then transferred into an NMR tube for 31 P NMR analysis. All NMR spectra of MWLs and LCCs were acquired on a Bruker AVANCE III 600 MHz spectrometer (Bruker, Biospin, Switzerland) equipped with a 5 mm BBO probe using an inverse gated proton decoupling sequence.
For comparison, the wet chemistry, alkaline nitrobenzene oxidation, was applied to the extractive-free ginkgo shells (40-80 mesh) and LCCs according to the procedure reported by Chen [8]. Briefly, 10 mg of each sample was reacted with 4 mL 2 mol/L sodium hydroxide and 0.25 mL nitrobenzene in a stainless steel bomb at 170 • C for 2 h. Then, the bomb was cooled in cold water immediately and 1 mL of 0.1 mol/L sodium hydroxide solution containing 3-ethoxy-4-hydroxybenzaldehyde (0.3 g/L) was used as the internal standard. The mixture was extracted three times with dichloromethane. The aqueous phase was acidified with 4 mol/L HCl to pH 1 and extracted twice with dichloromethane and once with ethyl ether. The combined organic phase was extracted with 20 mL deionized water and the organic phase was dried by anhydrous sodium sulfate overnight. After removing the insoluble inorganic materials by filtration, the solution was evaporated to dryness and silylated using N,O-bis(trimethylsilyl) acetamide at 100 • C for 10 min. The silylated samples were analyzed by gas chromatography (Plus 2010) equipped with a flame ionization detector and SH-Rtx-5 column (Shimazu Co., Kyoto, Japan).

Chemical and Elemental Composition
The main chemical components of ginkgo shells (ML and FZ), MWLs (MWL ML and MWL FL ), and LCCs (LCC ML and LCC FZ ) are given in Table 1. The amount of lignin (42%) and xylan (20%) in ginkgo shells was much higher than that of lignin (25-30%) and xylan (5-10%) in general softwood species [25], so it is reasonable to deduce that the lignin-xylan complex is one of the main covalent forms of LCC in ginkgo shells. The high lignin and xylan content may be one of the reasons that the rigidity of ginkgo shells is very high. The content of glucan in ginkgo shells is only about 20%, which means that the amount of cellulose in ginkgo shells is much less than that in general softwoods. Research has pointed out that Ginkgo biloba woods contain about 41% cellulose, 33% lignin, and 26% hemicelluloses, and the predominant polysaccharides in hemicelluloses are glucomannan and methylglucuronoxylan [26]. The main chemical composition of Ginkgo biloba leaves is terpene trilactones, flavonoids, and other trace constitutes [27]. These results suggested that the chemical composition of ginkgo shells is rather different from that of ginkgo woods and leaves. The yield of isolated MWL ML and MWL FZ , on the basis of total lignin in ginkgo shells, was only 17.5% and 15.3%, respectively. Due to the high rigidity of ginkgo shells and the fact that the lignin is mainly deposited in secondary cell walls [2], the ball milling degree in this work (2 h) may not completely destroy the cell walls, resulting in low-level lignin being extracted out. However, the purity of both MWLs was over 90%, meeting the need of structural analysis. The main polysaccharides in LCCs were glucan and xylan, and expectedly, the LCCs had a higher acid soluble lignin content than raw materials and MWLs owing to the low molecular weight of lignin in LCCs.
MWL ML and MWL FZ exhibited a similar elemental composition, as shown in Table 2. The N and S elements were present in MWLs besides the predominant C, H, and O elements. During lignification, the bio-enzymes such as phenylalanine ammonia-lyase, cinnamate 4-hydroxylase, 4-coumarate: CoA ligase, and p-coumarate 3-hydroxylase play an important role in the formation of lignin units and interunit linkages [28]. Ginkgo shells used in this work were extracted by benzene-ethanol solvent and no specific step was carried out to remove protein. Therefore, the N and S elements in MWLs could be mainly derived from protein.

Molecular Weight Distribution
Technically, the molecular weight distributions are dependent on the isolation and detection methods. The values of the weight-average (M w ), number-average molecular weights (M n ), and the polydispersity (M w /M n ) of MWL ML and MWL FZ are given in Table 2. MWL ML and MWL FZ exhibited similar M w , with a relative value of 12,130 Da and 11,550 Da, respectively. Both MWLs show a broad molecular weight distribution with the polydispersity data of 3.19 and 2.94 for MWL ML and MWL FZ , respectively.

1 H NMR Spectra of MWLs
The 1 H NMR spectra of acetylated MWL ML and MWL FZ are presented in Figure 2. Only small differences were found between MWL ML and MWL FZ . The broad signal around 7.3-7.6 ppm confirmed the presence of the p-coumarate-type structure, C α = O groups, and p-hydroxyphenyl (H) units in both MWLs [29]. Signals of H α from β-O-4 and β-5 interunit linkages could be found at 5.9 and 5.5 ppm, respectively, and the signals' intensity implied that β-O-4 were the prominent linkages. The proton signals from β-β linkages were not evident compared with that from β-5 , indicating that β-5 bonds may be the main condensed structure. -OCH 3 is an important functional group of lignin, which accounted for about 13.3% and 14.3% of MWL ML and MWL FZ , respectively, according to the calculation methods described by Mousavioun and Doherty [14]. The analysis of elements, molecular weight, and -OCH 3 groups gives the MWL ML and MWL FZ molecular formula C 9 H 7.93 O 2.73 (OCH 3 ) 0.81 and C 9 H 7.87 O 2.76 (OCH 3 ) 0.88 , respectively, which is important information on lignin structure for biorefinery and chemical modification.

2D HSQC NMR Spectra of MWLs
HSQC NMR is an effective and powerful tool used to probe the structure of lignin and its derivatives, and it can determine specific carbon functionalities that are unable to be identified in 13 C and 1 H spectra. The side-chain (δC/δH 90-50/6.0-2.5) and aromatic (δC/δH 160-90/8.0-6.0) regions of the HSQC NMR spectra of MWLML and MWLFZ are shown in Figure 3, and the structure of the main lignin substructures are depicted in Figure 4. The cross-signals related to the structural units and linkages in the spectra were assigned according to the published papers [30][31][32][33]. The side-chain regions of MWLML and MWLFZ in the HSQC NMR spectra were similar. Correlation peaks from methoxyl and β-O-4′ substructures (A, A′) were the most prominent in the HSQC spectra of MWLML and MWLFZ. The signals for the Cβ-Hβ correlations of γ-acylated β-O-4′ substructures (A′) linked to guaiacyl (G) units (A′β(G)) were clearly observed at δC/δH 81.6/4.67, indicating a high acylation extent of G-lignin units in ginkgo shells. Correlation signals from α,β-diaryl ether linkages were not detected in both spectra of MWLML and MWLFZ, indicating that α,β-diaryl ether was undetectable or present at a very low level in ginkgo shells, although they are easily detected in some herbaceous lignin preparations [34,35]. Other substructures were also clearly visible in the HSQC NMR spectra of the MWLs, including signals for phenylcoumarans (B), resinols (C), and dibenzodioxocins (D), and the small contour revealed that the amount of these substructures is low. Additionally, the minor signal from the Cα-Hα correlation of spirodienones (F) was observed in the HSQC NMR spectrum of MWLML, but was absent in that of MWLFZ. It indicated that MWLML has a higher content of β-1′ and α-O-α′ linkages than MWLFZ. Polysaccharide signals, dominated by hemicellulose correlations, were observed in the side-chain regions of the 2D HSQC spectra of MWLML and MWLFZ. The cross-peak signals of X2 (δC/δH 72.6/3.05), X3 (δC/δH 73.8/3.25), and X4 (δC/δH 75.4/3.53) were assigned to the C2-H2, C3-H3, and C4-H4 correlations of β-D-xylopyranoside. These signals indicated that xylan was hardly removed from the lignin structure and the covalent bonds were present between them. It was in line with the chemical components analysis (Table 1) which demonstrated that the content of xylan was higher than that of other polysaccharides in MWLs.

2D HSQC NMR Spectra of MWLs
HSQC NMR is an effective and powerful tool used to probe the structure of lignin and its derivatives, and it can determine specific carbon functionalities that are unable to be identified in 13 C and 1 H spectra. The side-chain (δ C /δ H 90-50/6.0-2.5) and aromatic (δ C /δ H 160-90/8.0-6.0) regions of the HSQC NMR spectra of MWL ML and MWL FZ are shown in Figure 3, and the structure of the main lignin substructures are depicted in Figure 4. The cross-signals related to the structural units and linkages in the spectra were assigned according to the published papers [30][31][32][33]. The side-chain regions of MWL ML and MWL FZ in the HSQC NMR spectra were similar. Correlation peaks from methoxyl and β-O-4 substructures (A, A ) were the most prominent in the HSQC spectra of MWL ML and MWL FZ . The signals for the C β -H β correlations of γ-acylated β-O-4 substructures (A ) linked to guaiacyl (G) units (A β(G) ) were clearly observed at δ C /δ H 81.6/4.67, indicating a high acylation extent of G-lignin units in ginkgo shells. Correlation signals from α,β-diaryl ether linkages were not detected in both spectra of MWL ML and MWL FZ , indicating that α,β-diaryl ether was undetectable or present at a very low level in ginkgo shells, although they are easily detected in some herbaceous lignin preparations [34,35]. Other substructures were also clearly visible in the HSQC NMR spectra of the MWLs, including signals for phenylcoumarans (B), resinols (C), and dibenzodioxocins (D), and the small contour revealed that the amount of these substructures is low. Additionally, the minor signal from the C α -H α correlation of spirodienones (F) was observed in the HSQC NMR spectrum of MWL ML , but was absent in that of MWL FZ . It indicated that MWL ML has a higher content of β-1 and α-O-α linkages than MWL FZ . Polysaccharide signals, dominated by hemicellulose correlations, were observed in the side-chain regions of the 2D HSQC spectra of MWL ML and MWL FZ . The cross-peak signals of X 2 (δ C /δ H 72.6/3.05), X 3 (δ C /δ H 73.8/3.25), and X 4 (δ C /δ H 75.4/3.53) were assigned to the C 2 -H 2 , C 3 -H 3 , and C 4 -H 4 correlations of β-D-xylopyranoside. These signals indicated that xylan was hardly removed from the lignin structure and the covalent bonds were present between them. It was in line with the chemical components analysis (Table 1) which demonstrated that the content of xylan was higher than that of other polysaccharides in MWLs. The main signals in the aromatic regions of the 2D HSQC NMR spectra of MWLs corresponded to the G and H units. Prominent signals assigned to p-coumarate (PCA) and ferulate (FA) structures were also observed, although they are hardly detected in general softwood lignin. The C2-H2 and C6-H6 correlations of FA were observed at δC/δH 111.1/7.34 and 123.3/7.20, respectively. Only the C3,5-H3,5 correlations from PCA were detected at δC/δH 115.5/6.76, and the C2,6-H2,6, Cα-Hα, and Cβ-Hβ correlations were hardly detected in the HSQC spectra. The results revealed that the degree of reduction and the biosynthesis pathway of FA in ginkgo shells may be different from that of PCA. During lignification, the first step in the reduction of FA is the activation of the carboxyl group via the formation of a CoA ester. FA is converted to feruloyl adenylate, which is subsequently converted to feruloyl-caffeate by CoA. Similarly, hydroxycinnamoyl-CoA is reduced to the corresponding aldehydes by p-hydroxycinnamoyl-CoA reductase. The reactions are catalyzed by cinnamate:CoA ligase, which is distributed in various higher plants [36]. Although PCA and FA are effective substrates in the reactions, FA provides the initiation sites from which the lignification event in the cell wall begins and PCA is present via lignification using monolignol conjugation [37]. Therefore, the biosynthesis pathway of FA is different from that of PCA, resulting in different C-H correlations in the 2D HSQC spectra. Other signals in the aromatic regions of the HSQC NMR spectra of MWLs are from the unsaturated side-chains of cinnamyl alcohol end-groups and cinnamaldehyde endgroups. The signal of Cα-Hα and Cβ-Hβ correlations from cinnamyl alcohol end-groups was detected at δC/δH 128.3/6.44 and 128.3/6.23, respectively, and that from cinnamaldehyde end-groups was detected at 153.3/7.59 and 126.1/6.76, respectively. The main signals in the aromatic regions of the 2D HSQC NMR spectra of MWLs corresponded to the G and H units. Prominent signals assigned to p-coumarate (PCA) and ferulate (FA) structures were also observed, although they are hardly detected in general softwood lignin. The C 2 -H 2 and C 6 -H 6 correlations of FA were observed at δ C /δ H 111.1/7.34 and 123.3/7.20, respectively. Only the C 3,5 -H 3,5 correlations from PCA were detected at δ C /δ H 115.5/6.76, and the C 2,6 -H 2,6 , C α -H α , and C β -H β correlations were hardly detected in the HSQC spectra. The results revealed that the degree of reduction and the biosynthesis pathway of FA in ginkgo shells may be different from that of PCA. During lignification, the first step in the reduction of FA is the activation of the carboxyl group via the formation of a CoA ester. FA is converted to feruloyl adenylate, which is subsequently converted to feruloyl-caffeate by CoA. Similarly, hydroxycinnamoyl-CoA is reduced to the corresponding aldehydes by p-hydroxycinnamoyl-CoA reductase. The reactions are catalyzed by cinnamate:CoA ligase, which is distributed in various higher plants [36]. Although PCA and FA are effective substrates in the reactions, FA provides the initiation sites from which the lignification event in the cell wall begins and PCA is present via lignification using monolignol conjugation [37]. Therefore, the biosynthesis pathway of FA is different from that of PCA, resulting in different C-H correlations in the 2D HSQC spectra. Other signals in the aromatic regions of the HSQC NMR spectra of MWLs are from the unsaturated side-chains of cinnamyl alcohol end-groups and cinnamaldehyde end-groups. The signal of C α -H α and C β -H β correlations from cinnamyl alcohol end-groups was detected at δ C /δ H 128.3/6.44 and 128.3/6.23, respectively, and that from cinnamaldehyde end-groups was detected at 153.3/7.59 and 126.1/6.76, respectively.

2D HSQC NMR Spectra of LCCs
Almost all wood lignin is associated with polysaccharides, although less LCC exists in the plant. LCC limits the separation of lignin and carbohydrates and the bioconversion in biorefining. Therefore, in view of theory and practice, it is vitally important to understand the native LCC structure in the lignocellulosic biomass. As shown in Figure 5, various signals from the associated carbohydrates could be found in the HSQC spectra of LCCs. Assignment of these polysaccharide signals is listed in Table 3

2D HSQC NMR Spectra of LCCs
Almost all wood lignin is associated with polysaccharides, although less LCC exists in the plant. LCC limits the separation of lignin and carbohydrates and the bioconversion in biorefining. Therefore, in view of theory and practice, it is vitally important to understand the native LCC structure in the lignocellulosic biomass. As shown in Figure 5, various signals from the associated carbohydrates could be found in the HSQC spectra of LCCs. Assignment of these polysaccharide signals is listed in Table 3. The signals of β-D-xylopyranoside units (X 2 , X 3 , and X 4 ) detected in MWLs were also detected in LCCs. The respectively. These results suggested that the O-acetyl-β-D-xylopyranoside and β-D-mannopyranoside were the main polysaccharides associated with lignin, and the acetyl groups frequently acylate the C 2 and C 3 positions. You et al. [38] pointed out that O-acetyl-arabino-4-O-methylglucuronoxylan was the main associated polysaccharide in Björkman LCC from gramineous Arundo donax Linn. Yuan et al. [39] reported that the acetylated 4-O-methylgluconoxylan was the main associated carbohydrate in poplar LCC. These results indicated that the structural properties of LCC are dependent on the plant species, resulting in different biorefinery processes, such as those influencing the efficiency of enzymatic saccharification and the interaction of lignin with cellulase [40]. 101.3/4.28, and 98.3/4.72, respectively. In addition, the C2-H2 correlations from 2-O-acetyl-β-Dmannopyranoside (M22) and 3-O-acetyl-β-D-mannopyranoside (M23) were detected at δC/δH 70.3/5.27 and 73.6/4.93, respectively. These results suggested that the O-acetyl-β-D-xylopyranoside and β-Dmannopyranoside were the main polysaccharides associated with lignin, and the acetyl groups frequently acylate the C2 and C3 positions. You et al. [38] pointed out that O-acetyl-arabino-4-Omethylglucuronoxylan was the main associated polysaccharide in Björkman LCC from gramineous Arundo donax Linn. Yuan et al. [39] reported that the acetylated 4-O-methylgluconoxylan was the main associated carbohydrate in poplar LCC. These results indicated that the structural properties of LCC are dependent on the plant species, resulting in different biorefinery processes, such as those influencing the efficiency of enzymatic saccharification and the interaction of lignin with cellulase [40].

in β-D-xylopyranoside/β-D-glucopyranoside
It is believed that phenyl glycoside (PhGlc) bonds, ester (Est), and benzyl ether (BE) are the main types of LCC linkages [16,41]. As shown in Figure 6, the cross-peaks of PhGlc linkages could be observed in the region of δ C /δ H 104.0-98.0/5.30-4.50. The C 1 -H 1 and C 3 -H 3 correlations for PhGlc linkages in the HSQC spectrum of LCC ML were clearly observed at δ C /δ H 100.8/5.15 and 101.5/4.90, respectively. However, only the C 3 -H 3 correlations in the spectrum of LCC FZ were detected. In addition, the C 2 -H 2 correlations for PhGlc linkages were not detected in both HSQC spectra of LCC ML and LCC FZ . It was reported that classic Björkman LCC preparation was preferable for the analysis of PhGlc linkages by comparing the HSQC spectra of Björkman LCC, LCC-AcOH, and enzymatic hydrolysis of Björkman LCC [38]. Therefore, the PhGlc linkages may be mainly present in Björkman LCC of ginkgo shells.
BE linkages could be detected in the region of δ C /δ H 82.5-80.0/5. 30-4.30. Researchers have pointed out that BE structure can be divided into two types: the linkages between the α-position of lignin and primary OH groups of carbohydrates (at C 6 of Glucan, Galactan and Mannan; C 5 of Arabinan) and linkages between the α-position of lignin and secondary OH groups of carbohydrates, mainly of the lignin-xylan type [42]. In the present study, the C α -H α correlations in the BE structure were found at δ C /δ H 81.6/4.64 (BE 1 ) and 81.4/5.04 (BE 2 ). Cross-peaks from benzyl ester linkages in the region of 77.0-75.0/6.20-6.00 were not detected in the LCCs.
The correlations for Est linkages cross-linked at C γ were clearly observed at δ C /δ H 66.0-62.0/4.50-4.00. However, the signals are easily overlapped with signals of lignin substructures in the HSQC spectra such as the FA and PCA [43]. In addition, the signals of γ-esters are also overlapped with signals of γ-acetyl lignin moieties (A ), especially in woody lignins. The 600 MHz spectrometer does not allow distinguishing between the γ-signals of LCC and acetyl γ-esters of lignin due to broad and overlapping signals in this area. A previous report has pointed out that a 950 MHz spectrometer with CryProbe™ can overcome this problem [41]. It is believed that phenyl glycoside (PhGlc) bonds, ester (Est), and benzyl ether (BE) are the main types of LCC linkages [16,41]. As shown in Figure 6, the cross-peaks of PhGlc linkages could be observed in the region of δC/δH 104.0-98.0/5.30-4.50. The C1-H1 and C3-H3 correlations for PhGlc linkages in the HSQC spectrum of LCCML were clearly observed at δC/δH 100.8/5.15 and 101.5/4.90, respectively. However, only the C3-H3 correlations in the spectrum of LCCFZ were detected. In addition, the C2-H2 correlations for PhGlc linkages were not detected in both HSQC spectra of LCCML and LCCFZ. It was reported that classic Björkman LCC preparation was preferable for the analysis of PhGlc linkages by comparing the HSQC spectra of Björkman LCC, LCC-AcOH, and enzymatic hydrolysis of Björkman LCC [38]. Therefore, the PhGlc linkages may be mainly present in Björkman LCC of ginkgo shells.
BE linkages could be detected in the region of δC/δH 82.5-80.0/5. 30-4.30. Researchers have pointed out that BE structure can be divided into two types: the linkages between the α-position of lignin and primary OH groups of carbohydrates (at C6 of Glucan, Galactan and Mannan; C5 of Arabinan) and linkages between the α-position of lignin and secondary OH groups of carbohydrates, mainly of the lignin-xylan type [42]. In the present study, the Cα-Hα correlations in the BE structure were found at δC/δH 81.6/4.64 (BE1) and 81.4/5.04 (BE2). Cross-peaks from benzyl ester linkages in the region of 77.0-75.0/6.20-6.00 were not detected in the LCCs.
The correlations for Est linkages cross-linked at Cγ were clearly observed at δC/δH 66.0-62.0/4.50-4.00. However, the signals are easily overlapped with signals of lignin substructures in the HSQC spectra such as the FA and PCA [43]. In addition, the signals of γ-esters are also overlapped with signals of γ-acetyl lignin moieties (A′), especially in woody lignins. The 600 MHz spectrometer does not allow distinguishing between the γ-signals of LCC and acetyl γ-esters of lignin due to broad and overlapping signals in this area. A previous report has pointed out that a 950 MHz spectrometer with CryProbe™ can overcome this problem [41].

Quantification of Lignin Structure and LCC Linkages
Quantification of the lignin structure and LCC linkages is very important for providing comprehensive information about lignin architecture and reactivity, as well as for the biorefining process of lignocellulosic biomass. It will give guidance for the functional and chemical modification of lignin. 13 C NMR spectroscopy is a reliable tool for lignin characterization. However, for the quantification of LCC linkages, appropriate internal references are needed to translate the relative values obtained from the integration of the HSQC spectra to absolute values. Due to the high stability of lignin aromatic rings, the number of specific structures per aromatic ring (/Ar), which is equivalent to one lignin monomeric unit, was often selected as the internal standard reference [19]. The values in the 13 C NMR spectra (Figure 7) were related to the resonance of the aromatic carbons (163-103 ppm), which was set as 6.0. To quantify the LCC linkages, the values obtained from the integration of three clusters at 103-96 ppm, 90-78 ppm, and 65-58 ppm in the corresponding 13 C NMR spectra were used. These clusters contain the signals of PhGlc, BE, and Est linkages, correspondingly. The amounts of LCC linkages were calculated per Ar as follows [19]: where Est, BE, and PhGlc are the amounts of γ-ester, benzyl ether, and phenyl glycoside linkages (per Ar); 2D Est , 2D BE , and 2D PhGlc are the resonance of the signals of γ-ester, benzyl ether, and phenyl glycoside linkages in the 2D NMR spectra; 2D 65-58/5.0-2.5 , 2D 90-78/5.7-3.0 , and 2D 103-96/5.5-3.8 are the total resonance of the corresponding clusters in the 2D NMR spectra; 13 C 65-58 , 13 C 90-78 , 13 C 103-96 , and 13 C 163-103 are the resonance of the corresponding cluster in the 13 C NMR spectra; and 6.0 is the amount of aromatic carbons per Ar. The results on the quantification of the lignin structure and LCC linkages are summarized in Table 4.

Quantification of Lignin Structure and LCC Linkages
Quantification of the lignin structure and LCC linkages is very important for providing comprehensive information about lignin architecture and reactivity, as well as for the biorefining process of lignocellulosic biomass. It will give guidance for the functional and chemical modification of lignin. 13 C NMR spectroscopy is a reliable tool for lignin characterization. However, for the quantification of LCC linkages, appropriate internal references are needed to translate the relative values obtained from the integration of the HSQC spectra to absolute values. Due to the high stability of lignin aromatic rings, the number of specific structures per aromatic ring (/Ar), which is equivalent to one lignin monomeric unit, was often selected as the internal standard reference [19]. The values in the 13 C NMR spectra (Figure 7) were related to the resonance of the aromatic carbons (163-103 ppm), which was set as 6.0. To quantify the LCC linkages, the values obtained from the integration of three clusters at 103-96 ppm, 90-78 ppm, and 65-58 ppm in the corresponding 13 C NMR spectra were used. These clusters contain the signals of PhGlc, BE, and Est linkages, correspondingly. The amounts of LCC linkages were calculated per Ar as follows [19]:    The amount of condensed structures (β-β , β-1 and β-5 ) in MWL ML (0.41/Ar) was lower than that in MWL FZ (0.44/Ar), indicating that MWL FZ had a higher condensation degree. Integration values of MWLs via 2D HSQC NMR also showed that the predominant aryl ether and condensed structure were β-O-4 and β-5 bonds, respectively. The condensed phenolic structure containing β-5 , 4-O-5 and 5-5 linkages is fairly resistant to oxidative degradation, even at higher temperatures [44]. Therefore, the high condensation degree may lead to ginkgo shells lignin having high recalcitrance in the process of biorefinery. However, the high condensation degree gives ginkgo shells lignin higher antioxidant activity than general softwoods lignin, which will play an important role in functional materials production. The amount of aliphatic and conjugated COOR groups was estimated to be 0. The amount of BE linkages was 0.008/Ar in ginkgo shells, which was similar to that in Birch [41] but was lower than that in Arundo donax Linn [38], poplar MWL, and mild acidolysis lignin [39]. The amount of phenyl glycoside linkages in LCC ML (0.035/Ar) was higher than that in LCC FZ (0.027/Ar). However, the amount of γ-ester linkages in LCC ML (0.026/Ar) was lower than that in LCC FZ (0.039/Ar). These results suggest that the properties of LCCs depend on the plant species and the degree of lignification.

31 P NMR Spectra of MWLs
The content of hydroxyl groups can be determined by 31 P NMR spectroscopy (see Figure 8), which offers important chemical reactivity and biological information. The results obtained from 31 P NMR are included in Table 5. The phenolic hydroxyl was mainly derived from G units, with the amount of 0.78 mmol/g and 0.69 mmol/g for MWL ML and MWL FZ , respectively, which is much lower than that in spruce (1.51 mmol/g), eucalyptus (2.09 mmol/g), and wheat straw (2.06 mmol/g) [15]. The results indicated that lignin in ginkgo shells has a high etherification and condensation degree. In consideration of the amount of β-O-4 linkages (40%) in MWL ML and MWL FZ , it is reasonable to deduce that the amount of α-O-4 and 4-O-5 bonds are the main condensed etherified structure. Although the low-level phenolic hydroxyl content in ginkgo shells lignin is beneficial to protect the fruit in ginkgo shells from pathogene and corruption, it reduces the application beyond its current roles, especially in biorefinery. The lower content of phenolic hydroxyl in MWL FZ compared to that in MWL ML implied the MWL FZ had a higher condensation degree, which is line with the analysis of the 1 H, 13 C, and 2D HSQC NMR. However, the MWL FZ had more carboxyl groups than MWL ML , suggesting that MWL FZ may have a higher oxidation degree than MWL ML . However, the amount of γ-ester linkages in LCCML (0.026/Ar) was lower than that in LCCFZ (0.039/Ar). These results suggest that the properties of LCCs depend on the plant species and the degree of lignification.

31 P NMR Spectra of MWLs
The content of hydroxyl groups can be determined by 31 P NMR spectroscopy (see Figure 8), which offers important chemical reactivity and biological information. The results obtained from 31 P NMR are included in Table 5. The phenolic hydroxyl was mainly derived from G units, with the amount of 0.78 mmol/g and 0.69 mmol/g for MWLML and MWLFZ, respectively, which is much lower than that in spruce (1.51 mmol/g), eucalyptus (2.09 mmol/g), and wheat straw (2.06 mmol/g) [15]. The results indicated that lignin in ginkgo shells has a high etherification and condensation degree. In consideration of the amount of β-O-4′ linkages (40%) in MWLML and MWLFZ, it is reasonable to deduce that the amount of α-O-4′ and 4-O-5′ bonds are the main condensed etherified structure. Although the low-level phenolic hydroxyl content in ginkgo shells lignin is beneficial to protect the fruit in ginkgo shells from pathogene and corruption, it reduces the application beyond its current roles, especially in biorefinery. The lower content of phenolic hydroxyl in MWLFZ compared to that in MWLML implied the MWLFZ had a higher condensation degree, which is line with the analysis of the 1 H, 13 C, and 2D HSQC NMR. However, the MWLFZ had more carboxyl groups than MWLML, suggesting that MWLFZ may have a higher oxidation degree than MWLML.

Nitrobenzene Oxidation
Nitrobenzene oxidation is a crucial supplement of NMR spectroscopy, which provides information on the aromatic rings and condensation degree of lignin. The yield and ratio of nitrobenzene oxidation products of ginkgo shells and LCCs are listed in Table 6. The lower products yield of FZ (1.79 mmol/g-lignin) compared to that of ML (1.64 mmol/g-lignin) suggested that lignin in FZ had a higher condensation degree than that in ML, being in line with the NMR analysis. As

Nitrobenzene Oxidation
Nitrobenzene oxidation is a crucial supplement of NMR spectroscopy, which provides information on the aromatic rings and condensation degree of lignin. The yield and ratio of nitrobenzene oxidation products of ginkgo shells and LCCs are listed in Table 6. The lower products yield of FZ (1.79 mmol/g-lignin) compared to that of ML (1.64 mmol/g-lignin) suggested that lignin in FZ had a higher condensation degree than that in ML, being in line with the NMR analysis. As shown in Figure 2 and Table 4, the amount of β-5 linkages was obviously higher than that of other condensed structures (β-1 , β-β ). Analysis of 31 P NMR of MWLs also exhibited a high amount of α-O-4 and 4-O-5 structures in ginkgo shells lignin, which resulted in the nitrobenzene oxidation products yield of both ginkgo shells being much lower than that in general softwoods [45,46]. Tamai et al. [45] pointed out that the total yield of vanillin and vanillic acid of cedar (Cryptomeria japonica) under nitrobenzene oxidation was 1.95-1.99 mmol/g-lignin, which is about 17% and 26% higher than that in ML and FZ, respectively. The high condensation degree of ginkgo shells lignin may also explain why the MWL yield (17.5% and 15.3% for MWL ML and MWL FZ , respectively) from ginkgo shells is low.
As shown in Table 6, the yield of H units in ginkgo shells was higher than that in Pine [46]. The structure of PCA in ginkgo shells is contributed to H units. Furthermore, p-coumaroyl-CoA 3-hydroxylase (C3H) is a monooxygenase that catalyzes the 3-hydroxylation of p-coumaroyl shikimate and p-coumaroyl quinate. C3H easily becomes a rate-limiting step in lignin biosynthesis and it is not surprising that an extreme reduction in C3H activity results in an increase of p-hydroxyphenyl monomers [47]. The inactivation of C3H further supports the involvement of hydroxycinnamic acid shikimate esters in the lignin biosynthetic pathway, causing the high content of H units in nitrobenzene oxidation products. The higher amount of acid soluble lignin in LCCs than that in raw materials (Table 1) implies that the lignin in LCCs has a lower molecular weight. Therefore, the condensation degree of lignin in LCCs was much lower than that in raw materials, causing the LCCs to have a higher nitrobenzene oxidation products yield than ginkgo shells. 2.14 ± 0.00 0.02 ± 0.00 0.10 ± 0.00 3.00 ± 0.00 95/1/4

Conclusions
The chemical composition, structural properties of lignin, and lignin-carbohydrate complex in ginkgo shells are rather different from those in general softwoods. Ginkgo shells analyzed in this work have a higher lignin and xylan content but less cellulose when compared with general softwoods. Isolated milled wood lignins are rich in guaiacyl units and have a high condensation and etherification degree. Although hardly detected in general softwoods lignin, Ferulates and p-coumarates are detected in ginkgo shells lignin, and the biosynthesis pathway of ferulates is rather different from that of p-coumarates.

Conflicts of Interest:
The authors declare no conflict of interest.