Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst

: Value-added utilization of biomass-derived 5-hydroxymethylfurfural (HMF) to produce useful derivatives is of great interest. In this work, extremely radiation resistant Deinococcus wulumuqiensis R12 was explored for the ﬁrst time as a new robust biocatalyst for selective oxidation of HMF to 5-hydroxymethylfuroic acid (HMFCA). Its resting cells exhibited excellent catalytic performance in a broad range ofpH and temperature values, and extremely high tolerance to HMF and the HMFCA product. An excellent yield of HMFCA (up to 90%) was achieved when the substrate concentration was set to 300 mM under the optimized reaction conditions. In addition, 511 mM of product was obtained within 20 h by employing a fed-batch strategy, a ﬀ ording a productivity of 44 g / L per day. Of signiﬁcant synthetic interest was the ﬁnding that the D. wulumuqiensis R12 cells were able to catalyze the selective oxidation of other structurally diverse aldehydes to their corresponding acids with good yield and high selectivity, indicating broad substrate scope and potential widespread applications in biotechnology and organic chemistry.


Introduction
The production of bio-fuels and chemicals from carbon-neutral and renewable biomass is attracting increasing interest [1][2][3][4][5]. Biomass is regarded as a sustainable resource from which some platform chemicals can be manufactured [6,7]. 5-hydroxymethylfurfural (HMF), derived from lignocellulosic materials via dehydration of carbohydrates, is one of the most important platform chemicals [8][9][10]. It has been listed as one of "Top 10+4" bio-based chemicals by the U.S. Department of Energy (DOE) [11], being applied in the synthesis of a variety of value-added pharmaceutical and biomaterial intermediates [12]. Due to its high reactivity, HMF is a versatile molecule that can be converted into various useful furan derivatives [12][13][14]. Its structure comprises a furan ring, an aldehyde group and a hydroxymethyl group which can be subjected to upgrading processes by selective redox reactions, leading to 5-hydroxymethylfuroic acid (HMFCA), 2,5-diformylfuran (DFF), In order to form HMFCA, selective oxidation of the aldehyde group in HMF is required, while the alcohol group is left intact. Chemoselective oxidation methods are mainly used in the synthesis of HMFCA from HMF, in which noble metal catalysts are generally used [12,[20][21][22]. Recently, HMF was selectively oxidized to HMFCA by an immobilized molybdenum complex in toluene within 3 h, with a yield of approximately 87% [23]. Han et al. reported a selective and mild photocatalytic method for HMFCA synthesis from HMF under ultraviolet and visible light conditions with a yield of 90-95% [24]. In addition, the conversion of HMF to HMFCA via the Cannizzaro reaction is of great value [25,26]. However, the maximal selectivity of HMFCA was 50% due to the formation of an equimolar by-product.
Biocatalytic oxidation of HMF to HMFCA represents a promising alternative to chemical methods [14,27]. Biocatalysis offers many advantages, such as mild, environmentally friendly reaction conditions and often excellent selectivity, as well as high efficiency. However, compared to chemical methods, there are only a few reports on biotransformation of HMF to selectively form HMFCA in the literature [28][29][30][31][32]. In seminal work, Sheldon et al. reported the chloroperoxidase-catalyzed oxidation of HMF affording HMFCA with a selectivity of 25-40% [31]. Krystof et al. reported lipase-mediated and peracid-assisted oxidation of the HMF process to produce HMFCA [32]. Recently, Li and co-workers made use of a molybdenum-dependent enzyme-xanthine oxidase from Escherichia coli-for the biocatalytic oxidation of HMF to form HMFCA, with 94% yield and 99% selectivity [29].
Relative to the use of isolated enzymes, we believe that, in HMF oxidation, whole-cell biocatalysts have advantages. They are not only inexpensive and relatively stable, but they also do not require cofactor regeneration [27,33]. Biocatalysis is more efficient when recombinant whole cells that overexpress the enzyme(s) important for catalysis are used [34]. However, employing whole-cell biocatalysts for HMF oxidation is still challenging due to the well-known toxicity of HMF to microbial cells [30]. In addition, due to the variety of enzymes in microbial cells many side reactions are likely to occur during the process of HMF oxidation with formation of HMFCA [28]. Hence, exploring highly tolerant and selective microbial strains is crucial for the biotransformation of HMF into value-added derivative. To our knowledge, there are only a few studies on whole-cell-catalyzed selective oxidation of HMF to form HMFCA in the literature [28,30]; processes that are accompanied by a certain amount of HMF derivatives as byproducts. It was reported that some Pseudomonas strains have an HMF degradation pathway, in which HMF is converted to Scheme 1. Catalytic biotransformation of 5-hydroxymethylfurfural (HMF) into high value derivatives.
In order to form HMFCA, selective oxidation of the aldehyde group in HMF is required, while the alcohol group is left intact. Chemoselective oxidation methods are mainly used in the synthesis of HMFCA from HMF, in which noble metal catalysts are generally used [12,[20][21][22]. Recently, HMF was selectively oxidized to HMFCA by an immobilized molybdenum complex in toluene within 3 h, with a yield of approximately 87% [23]. Han et al. reported a selective and mild photocatalytic method for HMFCA synthesis from HMF under ultraviolet and visible light conditions with a yield of 90-95% [24]. In addition, the conversion of HMF to HMFCA via the Cannizzaro reaction is of great value [25,26]. However, the maximal selectivity of HMFCA was 50% due to the formation of an equimolar by-product.
Biocatalytic oxidation of HMF to HMFCA represents a promising alternative to chemical methods [14,27]. Biocatalysis offers many advantages, such as mild, environmentally friendly reaction conditions and often excellent selectivity, as well as high efficiency. However, compared to chemical methods, there are only a few reports on biotransformation of HMF to selectively form HMFCA in the literature [28][29][30][31][32]. In seminal work, Sheldon et al. reported the chloroperoxidase-catalyzed oxidation of HMF affording HMFCA with a selectivity of 25-40% [31]. Krystof et al. reported lipase-mediated and peracid-assisted oxidation of the HMF process to produce HMFCA [32]. Recently, Li and co-workers made use of a molybdenum-dependent enzyme-xanthine oxidase from Escherichia coli-for the biocatalytic oxidation of HMF to form HMFCA, with 94% yield and 99% selectivity [29].
Relative to the use of isolated enzymes, we believe that, in HMF oxidation, whole-cell biocatalysts have advantages. They are not only inexpensive and relatively stable, but they also do not require cofactor regeneration [27,33]. Biocatalysis is more efficient when recombinant whole cells that overexpress the enzyme(s) important for catalysis are used [34]. However, employing whole-cell biocatalysts for HMF oxidation is still challenging due to the well-known toxicity of HMF to microbial cells [30]. In addition, due to the variety of enzymes in microbial cells many side reactions are likely to occur during the process of HMF oxidation with formation of HMFCA [28]. Hence, exploring highly tolerant and selective microbial strains is crucial for the biotransformation of HMF into value-added derivative. To our knowledge, there are only a few studies on whole-cell-catalyzed selective oxidation of HMF to form HMFCA in the literature [28,30]; processes that are accompanied by a certain amount of HMF derivatives as byproducts. It was reported that some Pseudomonas strains have an HMF degradation pathway, in which HMF is converted to HMFCA as an intermediate [35][36][37]. A careful literature search did not reveal any studies describing the use of this system for the production of HMFCA. In 2010, Koopman et al. reported the production of 2,5-furandicarboxylic acid (FDCA) from HMF by using recombinant P. putida S12_hmfH. As part of this biotransformation, HMFCA hardly accumulated, leading to a mixture of other metabolites [38,39]. Therefore, in the challenging quest to obtain large amounts of pure HMFCA, the use of the Pseudomonas strain metabolic pathway is not feasible. Moreover, long standing issues still exist, such as low substrate loading, substrate toxicity and insufficient selectivity, etc. Therefore, searching for new and robust biocatalytic systems with high selectivity is a demanding task.
Extremophiles are organisms that have evolved to thrive under one or more extreme adverse environmental conditions where other organisms cannot survive [40,41]. They are regarded as an ideal and valuable source of biocatalysts, allowing biotransformation under relatively harsh industrial conditions [42][43][44]. Nevertheless, employing whole-cells or isolated enzymes derived from extremophiles for biocatalysis in a general manner is just beginning to be implemented experimentally. Recently, a Deinococcus sp, designated as Deinococcus wulumuqiensis R12, was isolated from radiation-polluted soil [45,46]. Previous studies showed that it is phylogenetically more closely related to a prototype strain of the Deinococcus genus, namely Deinococcus radiodurans R1 [47]. It was found that this strain was capable of producing carotenoids with good yield, and related biosynthesis genes were subsequently cloned and heterogeneously expressed in E. coli. by Xu et al. [48]. Furthermore, its whole genome was sequenced by Huang et al. [49]. Recently, genes encoding heat shock proteins from D. wulumuqiensis R12 were introduced into Clostridium acetobutylicum ATCC824 in order to improve the robustness and butanol titers of host cells [50]. Considering the robustness of D. wulumuqiensis R12, it would be of great interest to explore the catalytic properties of its whole cells in biotransformation or bioconversion.
In this study, we report that the radiation resistant strain D. wulumuqiensis R12 that can indeed be used as a whole-cell biocatalyst in HMFCA synthesis by selective oxidation of HMF (Scheme 2). The catalytic properties of this strain were evaluated in the transformation of HMF, and the reaction conditions were optimized. In addition, the substrate scope of this new whole-cell biocatalyst was also investigated. HMFCA as an intermediate [35][36][37]. A careful literature search did not reveal any studies describing the use of this system for the production of HMFCA. In 2010, Koopman et al. reported the production of 2,5-furandicarboxylic acid (FDCA) from HMF by using recombinant P. putida S12_hmfH. As part of this biotransformation, HMFCA hardly accumulated, leading to a mixture of other metabolites [38,39]. Therefore, in the challenging quest to obtain large amounts of pure HMFCA, the use of the Pseudomonas strain metabolic pathway is not feasible. Moreover, long standing issues still exist, such as low substrate loading, substrate toxicity and insufficient selectivity, etc. Therefore, searching for new and robust biocatalytic systems with high selectivity is a demanding task.
Extremophiles are organisms that have evolved to thrive under one or more extreme adverse environmental conditions where other organisms cannot survive [40,41]. They are regarded as an ideal and valuable source of biocatalysts, allowing biotransformation under relatively harsh industrial conditions [42][43][44]. Nevertheless, employing whole-cells or isolated enzymes derived from extremophiles for biocatalysis in a general manner is just beginning to be implemented experimentally. Recently, a Deinococcus sp, designated as Deinococcus wulumuqiensis R12, was isolated from radiation-polluted soil [45,46]. Previous studies showed that it is phylogenetically more closely related to a prototype strain of the Deinococcus genus, namely Deinococcus radiodurans R1 [47]. It was found that this strain was capable of producing carotenoids with good yield, and related biosynthesis genes were subsequently cloned and heterogeneously expressed in E. coli. by Xu et al. [48]. Furthermore, its whole genome was sequenced by Huang et al. [49]. Recently, genes encoding heat shock proteins from D. wulumuqiensis R12 were introduced into Clostridium acetobutylicum ATCC824 in order to improve the robustness and butanol titers of host cells [50]. Considering the robustness of D. wulumuqiensis R12, it would be of great interest to explore the catalytic properties of its whole cells in biotransformation or bioconversion.
In this study, we report that the radiation resistant strain D. wulumuqiensis R12 that can indeed be used as a whole-cell biocatalyst in HMFCA synthesis by selective oxidation of HMF (Scheme 2). The catalytic properties of this strain were evaluated in the transformation of HMF, and the reaction conditions were optimized. In addition, the substrate scope of this new whole-cell biocatalyst was also investigated. Scheme 2. Chemoselective oxidation of MF to 5-hydroxymethylfuroic acid (HMFCA) with whole D. wulumuqiensis R12 cells.

Growing and Resting Deinococcus Cells as Catalysts in HMF Oxidation with Selective Formation of HMFCA
Similar to the prototype strain of the Deinococcus genus, D. radiodurans R1, D. wulumuqiensis R12 is also well known for its excellent ability to resist extremely high doses of gamma and UV radiation [45]. In order to explore its potential applications in biocatalysis, growing and resting cells of this strain were applied as biocatalysts in the conversion of HMF to form HMFCA. As shown in Figure  1a, 100 mM of the HMF substrate were converted almost completely within 12 h using resting cells, whereas growing cells gave only a 32% yield at a prolonged reaction time of 36 h. Resting cells enabled a much higher yield with more than 98% of HMFCA and a trace amount of 2,5-bis (hydroxymethyl) furan (BHMF) as sole byproduct, indicating excellent chemoselectivity in this biocatalytic process. Increasing substrate concentration further did not affect the selectivity of the resting cells ( Figure S2). Considering the reported degradation mechanism of HMF in microbial cells [51], it is reasonable to speculate that the intermediate HMF alcohol (from HMF reduction) was almost completely oxidized in a very short time to form the final HMFCA, or the HMF substrate was Scheme 2. Chemoselective oxidation of MF to 5-hydroxymethylfuroic acid (HMFCA) with whole D. wulumuqiensis R12 cells.

Growing and Resting Deinococcus Cells as Catalysts in HMF Oxidation with Selective Formation of HMFCA
Similar to the prototype strain of the Deinococcus genus, D. radiodurans R1, D. wulumuqiensis R12 is also well known for its excellent ability to resist extremely high doses of gamma and UV radiation [45]. In order to explore its potential applications in biocatalysis, growing and resting cells of this strain were applied as biocatalysts in the conversion of HMF to form HMFCA. As shown in Figure 1a, 100 mM of the HMF substrate were converted almost completely within 12 h using resting cells, whereas growing cells gave only a 32% yield at a prolonged reaction time of 36 h. Resting cells enabled a much higher yield with more than 98% of HMFCA and a trace amount of 2,5-bis (hydroxymethyl) furan (BHMF) as sole byproduct, indicating excellent chemoselectivity in this biocatalytic process. Increasing substrate concentration further did not affect the selectivity of the resting cells ( Figure S2). Considering the reported degradation mechanism of HMF in microbial cells [51], it is reasonable to speculate that the intermediate HMF alcohol (from HMF reduction) was almost completely oxidized in a very short time to form the final HMFCA, or the HMF substrate was oxidized directly-which constitutes a different mechanistic hypothesis. However, to validate this inference, more efforts need to be invested. In addition, we were pleased to discover the performance of two other Deinococcus strains stored in our lab, D. radiodurans R1 and Deinococcus xibeiensis R13, which were also used in the biocatalytic oxidation of HMF to HMFCA. It was found that both radiation resistant strains selectively oxidized HMF with formation of HMFCA. The conversions achieved by D. radiodurans R1 and D. xibeiensis R13 were slightly lower than that of D. wulumuqiensis R12 under the same reaction conditions ( Figure S3). These results suggest that the D. wulumuqiensis R12 cells act as a catalytic system with high activity and excellent chemoselectivity in the oxidation of HMF to HMFCA. Its catalytic properties were subsequently investigated in greater detail ( Figure 1). Figure 1b shows the influence of microbial cell dosage on HMFCA synthesis based on selective oxidation of HMF. The yield of HMFCA increased steadily from 18% to 99% with increasing cell dosage in the presence of 100 mM of HMF substrate. The maximal yield of 99% was achieved when the cell dosage reached 0.12 g/mL, and further increasing did not improve the HMFCA yield, indicating that the biocatalyst was potentially saturated by substrate under the given reaction conditions. Our results imply that the conversion of HMF to HMFCA correlates with the cell dosage employed  Figure S4). In addition, cell dosage had no significant effect on the selectivity of the reactions (>98%). A higher cell dosage may result in higher viscosity, however, which could impact mass transfer of the reaction mixture. Thus, the optimal cell dosage of 0.12 g/mL wet cells was used in subsequent experiments.

Effect of Temperature and pH on HMFCA Synthesis
The influence of temperature and pH on HMFCA synthesis in the whole-cell catalyzed oxidation of HMF was also studied. As shown in Figure 1c, the effect of reaction temperature on HMF selective oxidation was determined by performing the transformation at different temperatures. Remarkably, the microbial cell biocatalyst showed considerable activity at a broad temperature range, from 25 to 60 • C. The maximal substrate conversion of 79% was obtained at 35 • C after 4 h in the presence of 100 mM HMF substrate. In addition, even at 50 • C, 67% of the HMF substrate was converted to HMFCA, which is in accord with an early report that D. wulumuqiensis R12 has a broad growth temperature range [45]. Slightly decreased conversion is possibly due to the inactivation of the enzymes in the microbial cells at 60 • C. It should be mentioned that HMFCA was obtained as essentially the only oxidative product-with a yield of 99%-in the reaction within the temperature range of 25 • C to 60 • C, indicating excellent catalytic selectivity of the whole-cell biocatalyst. Considering the thermostability of cells and energy efficiency, a temperature of 35 • C was set for subsequent experiments.
In addition, we further studied the pH profile of the whole-cell catalyst in HMFCA synthesis via selective oxidation of HMF (Figure 1d). It was found that the microbial cells had a broadpH activity profile and exhibited a particularly good catalytic performance in the pH range of 5.0 to 10.0. The best yield of 81% was achieved in 100 mM phosphate buffer at a pH 7.0 after 4 h. Interestingly, a conversion percentage of 60% and 58% was obtained in phosphate buffer ofpH 5.0 and Gly-NaOH buffer ofpH 10.0, respectively, after a reaction time of 4 h. In addition, it appeared that the buffer types had a moderate influence on the conversion of HMF, as a yield of 54% was obtained in Tris-HCl buffer (pH 9.0), compared to 63% in Gly-NaOH buffer at the samepH . One should not be surprised that D. wulumuqiensis R12 cells are able to resist such harsh reaction conditions with extremepH values. In their studies, Wang et al. reported that the D. wulumuqiensis R12 strain is able to grow in a widepH range from 5.0 to 12.0 [45]. Compared to Comamonas testosterone SC1588, which has been applied in HMFCA synthesis from HMF [28], D. wulumuqiensis R12 cells showed higher tolerance to extremepH values. Therefore, the optimalpH value of 7.0 was selected for all subsequent experiments.

Inhibitory and Toxic Effect of Substrate
HMF is a well-known toxic inhibitor of microbial cells, inhibiting their growth and hindering their upgrading of HMF by whole-cell biocatalysis [52]. The catalytic performance of D. wulumuqiensis R12 cells towards HMF under varying concentrations was therefore tested. As shown in Figure 2a, HMFCA was synthesized in 99% yield within 12 h when the substrate concentration was 150 mM. However, the yield decreased slightly to 81% at the substrate concentration of 200 mM. The yield decreased gradually in the substrate concentration range of 250-1000 mM. Remarkably, 41% yield of HMFCA was obtained when the substrate concentration reached 500 mM, and a yield of 23% was observed at the substrate concentration of 1000 mM. The essentially complete selectivity remained almost constant at these varying substrate concentrations. It is well known that the cytotoxicity of HMF to microbial cells is a key parameter in whole-cell biocatalytic conversion of HMF. As shown in Figure 2b, the effect of substrate concentration on cell viability was investigated by using a cell viability assay. The microbial cells were incubated with varying concentrations of HMF under the usual reaction conditions, and the cell viability was subsequently measured using an Annexin V-FITC/PI Apoptosis Detection Kit. Microbial cells incubated in buffer without substrate under the same reaction condition were used as a control. Good cell viability (94%) was unexpectedly obtained in the presence of 400 mM substrate, which was not significantly different than that of the control. This result suggests that the microbial cells can tolerate as much as 400 mM of HMF substrate without losing viability. Further increasing HMF concentration to 500 mM led only to a slight decrease in cell viability to 83%. When 1000 mM of HMF substrate was used, 73% cell viability was still observed, indicating that D. wulumuqiensis R12 is extremely tolerant to HMF substrate. However, the conversion of HMF in the oxidation reaction decreased dramatically with increased substrate concentration. Since detailed toxic mechanisms of HMF to microbial cells are not completely understood, nor the reason for the extreme resistance of D. wulumuqiensis R12 cells to harsh environmental factors, the present results are not considered surprising.

Inhibitory and Toxic Effect of the HMFCA Product
In the oxidation of HMF to HMFCA, the product is an acidic compound harboring a carboxylic and hydroxyl group. In our work, the pH of the reaction system decreased over the reaction time due to the accumulation of the HMFCA product. This situation could conceivably become critical when high concentrations of substrate are produced. Thus, it would be of great interest and importance to investigate the possible inhibition and toxicity of the product towards microbial cells. Microbial cells incubated in phosphate buffer without the product was used as the control for such investigations. Based on the high cell viability (>93%), which was comparable to that of the control, there appeared to be no significant toxicity towards the microbial cells at HMFCA product concentrations less than 400 mM, as shown in Figure 2c. However, a product amount of 500 mM resulted in slight toxicity, as the cell viability value decreased to 87%. A further increased product concentration of 1000 mM led to significant toxicity of the microbial cells, with a viability of 84%. To our surprise, the data showed that the product toxicity to the viability of microbial cells was not as high as expected, even at extremely high concentrations. Considering that D. wulumuqiensis R12 is a robust strain, able to grow in a broad range ofpH values, one should not be surprised that the microbial cells are highly resistant to HMFCA production, with excellent cell viability at extremely high product concentrations. Further product toxicity tests with still higher concentrations were not performed, as the conversion of HMF was already very low at the substrate concentration of 1000 mM.

Manufacture of HMFCA Under Optimized Conditions
Obtaining large amounts of HFMCA is highly desired in biocatalytic oxidation of HMF by whole-cell biocatalysis, with great potential applications in industrial production. Therefore, further enhancement of the catalytic performance of D. wulumuqiensis R12 was investigated by optimizing the biocatalytic parameters of the conversion process. It was found that the HMFCA product yields were affected significantly by increasing the substrate concentration. For example, due to the known negative effect of HMF, the yield of HMFCA decreased significantly when HMF concentrations were higher than 300 mM (Figure 2a). Based on the catalytic properties of this strain, increasing the dosage of microbial cells in the reaction may further enhance the HMFCA yield. Recently, Zhang et al. reported that improved synthesis of HFMCA from HMF was obtained by tuning the pH of the reaction mixture using NaOH solution during the catalytic process [28]. Thus, we decided to employ the same strategy. In addition, it has been reported that adding furfural and furfural alcohol as inducers during cultivation of microbial cells for biocatalysis can trigger the expression of the enzymes responsible for HMF oxidation, which can facilitate HMFCA production. Therefore, three strategies (increasing microbial cell dosage, using inducing cells and tuningpH of reaction mixture) were applied in subsequent studies.
Increasing the dosage of microbial cells proved to be effective for enhancing the yield of HMFCA ( Figure S4a). For example, in the presence of 300 mM HMF, the yield of HMFCA increased considerably from 59% to 71% when the concentration of cells increased from 0.12 g/mL to 0.2 g/mL. A further increase in cells dosage was not performed considering the negative effect of higher viscosity in the reaction mixture. On the other hand, influencing the expression of cells by the use of furfural and furfural alcohol for enhancing the yield of HMFCA proved not to be effective ( Figure S4b). The reason for this might be that enzymes in D. wulumuqiensis R12 responsible for HMF oxidation are expressed constitutively. Finally,pH tuning was found to be an effective method for improving the yield of HMFCA ( Figure S4c). Thus, the pH of the reaction mixture was tuned to approximately 7.0 using a NaOH solution. Compared with the control withoutpH tuning, the HMFCA yield improved from 66% to 83% in the presence of 300 mM HMF substrate, and from 48% to 65% at an HMF concentration of 500 mM.
Therefore, both increasing the dosage of microbial cells and pH tuning was applied together for enhancing the production of HMFCA. As shown in Figure 3, at a high HMFCA concentration, a yield of 90% was achieved after 36 h when the substrate concentration was 300 mM. This demonstrates the considerable effectiveness of the combined strategy. In addition, the oxidative conversion of HMF to HMFCA reached 80% in the presence of 350 mM HMF after 48 h. When the concentration of HMF was set to 500 mM, 66% of the substrate was still converted after 48 h, but further prolonged reaction times did not lead to an increase in HMFCA yield.  Therefore, both increasing the dosage of microbial cells and pH tuning was applied together for enhancing the production of HMFCA. As shown in Figure 3, at a high HMFCA concentration, a yield of 90% was achieved after 36 h when the substrate concentration was 300 mM. This demonstrates the considerable effectiveness of the combined strategy. In addition, the oxidative conversion of HMF to HMFCA reached 80% in the presence of 350 mM HMF after 48 h. When the concentration of HMF was set to 500 mM, 66% of the substrate was still converted after 48 h, but further prolonged reaction times did not lead to an increase in HMFCA yield.
Compared with previous reported biocatalysis results, the data obtained in this work proved to be more efficient and selective because of a higher substrate concentration and simpler catalytic process. As shown in Table 1, the substrate concentrations used in the reported biocatalytic routes were still very low and co-enzymes were usually required when isolated enzymes were applied. Although C. testosterone SC1588 cells also display good selectivity and high HMF tolerance, its catalytic performance is highly sensitive to pH [28]. Thus, a considerable amount of histidine co-substrate is required for efficient selective oxidation of HMF. The extreme environment-derived D. wulumuqiensis R12 strain used in this work exhibited excellent resistance to high pH and temperatures, and proved to be a robust biocatalyst for HMFCA synthesis by way of selective oxidation of HMF.  Compared with previous reported biocatalysis results, the data obtained in this work proved to be more efficient and selective because of a higher substrate concentration and simpler catalytic process. As shown in Table 1, the substrate concentrations used in the reported biocatalytic routes were still very low and co-enzymes were usually required when isolated enzymes were applied. Although C. testosterone SC1588 cells also display good selectivity and high HMF tolerance, its catalytic performance is highly sensitive topH [28]. Thus, a considerable amount of histidine co-substrate is required for efficient selective oxidation of HMF. The extreme environment-derived D. wulumuqiensis R12 strain used in this work exhibited excellent resistance to highpH and temperatures, and proved to be a robust biocatalyst for HMFCA synthesis by way of selective oxidation of HMF.

Efficient Synthesis of HMFCA by a Fed-Batch Strategy
As mentioned above, excellent yields of HMFCA from selective oxidation of HMF were obtained under optimized conditions. It is highly desirable to manufacture HMFCA on a large scale in an effort to create a practical biocatalytic process. Thus, by applying a fed-batch strategy, in which HMF substrate was added continuously, the accumulation of high concentrations of product was achieved.  Figure 4 shows the results of biocatalytic synthesis of high concentrations of HMFCA. It was found that 511 mM of product was produced within 20 h after three-batch feeding of HMF, affording a total yield of 85% and a productivity of approximately 44 g/L per day. Only 4 mM of BHMF was observed as the sole byproduct (<1%). Chemoselectivity towards the target product reached more than 99%. In addition, a decrease in yield of HMFCA in each batch feeding was observed, indicating possible substrate and/or product inhibition in the whole-cell biocatalyst. An attempt to improve the yield of HMFCA further in this fed-batch process was performed by prolonging the reaction time, but no significant improvement was observed (Data not shown).

Efficient Synthesis of HMFCA by a Fed-Batch Strategy
As mentioned above, excellent yields of HMFCA from selective oxidation of HMF were obtained under optimized conditions. It is highly desirable to manufacture HMFCA on a large scale in an effort to create a practical biocatalytic process. Thus, by applying a fed-batch strategy, in which HMF substrate was added continuously, the accumulation of high concentrations of product was achieved. Figure 4 shows the results of biocatalytic synthesis of high concentrations of HMFCA. It was found that 511 mM of product was produced within 20 h after three-batch feeding of HMF, affording a total yield of 85% and a productivity of approximately 44 g/L per day. Only 4 mM of BHMF was observed as the sole byproduct (<1%). Chemoselectivity towards the target product reached more than 99%. In addition, a decrease in yield of HMFCA in each batch feeding was observed, indicating possible substrate and/or product inhibition in the whole-cell biocatalyst. An attempt to improve the yield of HMFCA further in this fed-batch process was performed by prolonging the reaction time, but no significant improvement was observed (Data not shown).

Exploring the Substrate Scope of D. wulumuqiensis R12
In order to examine the substrate spectrum of this novel whole-cell biocatalyst, a set of structurally unique aldehyde compounds was applied in the oxidation reactions catalyzed by D. wulumuqiensis R12 cells (Scheme 3). Considering possible solubility and toxicity effects, proper concentrations of these aldehyde compounds were applied. The results showed that the whole cells of the strain readily accept furfural as a substrate, thereby enabling an efficient synthesis of furoic acid (>99% yield). Furoic acid can be used in the pharmaceutical, agrochemical, and flavor industries [9]. In the case of aldehydes containing an additional hydroxyl group, the microbial cells proved to be strictly chemoselective for the aldehyde group, affording the corresponding carboxylic acids with good to excellent yields ( Table 2). For example, oxidation of DFF and FFCA to FDCA was achieved

Exploring the Substrate Scope of D. wulumuqiensis R12
In order to examine the substrate spectrum of this novel whole-cell biocatalyst, a set of structurally unique aldehyde compounds was applied in the oxidation reactions catalyzed by D. wulumuqiensis R12 cells (Scheme 3). Considering possible solubility and toxicity effects, proper concentrations of these aldehyde compounds were applied. The results showed that the whole cells of the strain readily accept furfural as a substrate, thereby enabling an efficient synthesis of furoic acid (>99% yield). Furoic acid can be used in the pharmaceutical, agrochemical, and flavor industries [9]. In the case of aldehydes containing an additional hydroxyl group, the microbial cells proved to be strictly chemoselective for the aldehyde group, affording the corresponding carboxylic acids with good to excellent yields ( Table 2). For example, oxidation of DFF and FFCA to FDCA was achieved with 100% and 63% yields, respectively. Due to solubility issues, higher DFF substrate concentrations were not applied. We also discovered that the aldehyde group of vanillin could be selectively oxidized by the microbial cells to vanillic acid. Vanillic acid has important applications in the pharmaceutical industry, and also as a monomer in polyester synthesis [54]. In addition, terephthalic acid and p-hydroxybenzoic acid were also prepared by selective oxidation of the corresponding aldehydes. In short, the results showed that D. wulumuqiensis R12 as a biocatalyst has an amazingly broad substrate spectrum.
concentrations were not applied. We also discovered that the aldehyde group of vanillin could be selectively oxidized by the microbial cells to vanillic acid. Vanillic acid has important applications in the pharmaceutical industry, and also as a monomer in polyester synthesis [54]. In addition, terephthalic acid and p-hydroxybenzoic acid were also prepared by selective oxidation of the corresponding aldehydes. In short, the results showed that D. wulumuqiensis R12 as a biocatalyst has an amazingly broad substrate spectrum. Scheme 3. Aldehyde substrates used and products obtained in biocatalytic oxidation by D. wulumuqiensis R12 whole cells.

Chemicals and Strains
Extremely radiation resistant strains D. wulumuqiensis R12 (DSM 28115T ), D. radiodurans R1 (ATCC NO.13939), and D. xibeiensis R13 (NRBC 105666T ) were acquired from Zhi-Dong Zhang at the Institute of Microbiology, Xinjiang Academy of Agricultural Sciences in China and stored in our Aldehyde substrates used and products obtained in biocatalytic oxidation by D. wulumuqiensis R12 whole cells.

Chemicals and Strains
Extremely radiation resistant strains D. wulumuqiensis R12 (DSM 28115T), D. radiodurans R1 (ATCC NO.13939), and D. xibeiensis R13 (NRBC 105666T) were acquired from Zhi-Dong Zhang at the Institute of Microbiology, Xinjiang Academy of Agricultural Sciences in China and stored in our laboratory. The GenBank accession number for the 16S rDNA sequence was KJ784486, while the whole genome sequence was APCS01000000.

Cultivation of D. wulumuqiensis R12 Cells
The glycerol stock of D. wulumuqiensis R12 was pre-cultivated at 30 • C, 200 rpm for 24 h in tryptone glucose yeast extract (TGY) medium containing 0.5% tryptone, 0.1% glucose, and 0.3% yeast extract. Then, 1% of the overnight preculture was transferred to fresh TGY medium. The culture was incubated at 30 • C, 200 rpm for 48 h and was centrifugated under 5000 rpm for 10 min to harvest cells. The cell pellet was washed twice with 100 mM phosphate buffer (pH 7.4) and resuspended in phosphate buffer with a final cell concentration of 0.12 g/mL (cell wet weight).

General Procedure for the Biocatalytic Oxidation of Aldehyde Substrates
Five milliliters of phosphate buffer (0.1 M,pH 7.0) containing the designated amounts of microbial cells (cell wet weight) and substrates was incubated at 35 • C and 850 rpm for a given reaction time. Aliquots of the reaction mixture were withdrawn at specified reaction times and diluted with the phosphate buffer prior to high-performance liquid chromatography (HPLC) assays. The conversion of HMF and other aldehydes by biocatalytic oxidation was defined as the percentage of the consumed substrate amount in the initial amount. The selectivity of the reaction was defined as the ratio of HMFCA product amount to the sum of all the products. The yield was defined as the percentage of the measured product amount in the theoretical product amount based on the initial amount of HMF.

Analytical Method
The reaction products were analyzed by HPLC following a previously reported method with slight modifications [28]. Briefly, a reverse-phase HPLC (Thermo Fisher ultimate 3000), equipped with Sepax GP-C18 column (4.6 mm × 250 mm, 5 µm), was used at 25 • C. The mobile phase was the gradient of acetonitrile in 20 mM KH 2 PO 4 (pH 6.0) at a flow of 1.0 mL min −1 , increasing from 10% to 24% within 7 min and from 24% to 10% within 3 min. The HPLC retention time of the HMFCA product and HMF were 2.90 min and 6.20 min, respectively. All experiments were performed in triplicate and mean values are presented. Data are expressed as the mean ± standard deviation. Duncan's multiple range test (using SPSS software 16.0, Chicago, IL, USA) was used to analyze the statistical significance of differences between the groups. A significance difference was judged to exist at a level of p < 0.05. HPLC runs are shown in Figure S1.

Cell Viability Assay
Cell viability assay experiments were performed using an Annexin V-FITC/PI Apoptosis Detection Kit and flow cytometry following the manufacturers' instructions. Cell viability was determined using ACEA NovoCyte Flow Cytometer (ACEA Biosciences, Inc., San Diego, CA, USA) with the excitation light and emission light wavelengths set at 488 nm and 530 nm, respectively. Data were collected and analyzed using NovoExpress software. The cell viability of D. wulumuqiensis R12 when using HMF as the substrate is presented as the percentage of living cells to the total amount of cells.

Synthesis of HMFCA by the Substrate Fed-Batch Feeding Process
Five milliliters of 100 mM phosphate buffer,pH 7.0, which contained 150 mM HMF substrate and 0.2 g/mL of microbial cells, was incubated at 35 • C and 850 rpm. After 5 h, 0.75 mmol of HMF was repeatedly added to the reaction mixture. During the whole biocatalytic process, the pH of the reaction mixture was adjusted to the range of 7.0-8.0 with NaOH solution and the concentration of substrate and products was analyzed by HPLC.

Conclusions
Herein, we successfully explored for the first time the use of a radiation resistant D. wulumuqiensis R12 strain as a whole-cell biocatalyst for the efficient synthesis of HMFCA from HMF. The whole cells of this strain proved to be highly tolerant to HMF and the product, HMFCA. The whole-cell system is an excellent biocatalyst for the selective oxidation of HMF. An excellent yield of HMFCA of up to 90% was achieved within 36 h in the presence of 300 mM HMF substrate under optimized conditions. A yield of 80% to 66% was obtained when the substrate concentration increased from 350 mM to 500 mM, while the selectivity towards HMFCA remained at approximately 98%. In addition, up to 511 mM of HMFCA was synthesized in 20 h via a fed-batch method, resulting in a productivity of 44 g/L per day. Thus, D. wulumuqiensis R12 cells are a promising catalyst in the biocatalytic process of HMF upgrading. Moreover, the cells were able to transform a set of structurally different aldehyde compounds into their corresponding carboxylic acids with good to excellent selectivity. Since the genome sequence of this strain has been sequenced, exploring the genes that encode the enzymes responsible for HMFCA synthesis from HMF has become feasible in future work. The catalytic properties of these microbial cells can also be further engineered by introduction of other oxidases to form a cell factory for HMF biotransformation. Furthermore, this strain may also have potential applications for the biodetoxification of lignocellulosic hydrolysates in the process of biofuel production. Discovery of D. wulumuqiensis R12 as an efficient biocatalyst broadens the toolbox of biocatalysts for the biotransformation of HMF into value-added derivatives and will further facilitate the utilization of biomass for the production of useful chemicals and biofuels.