Industrial Application of 2-Oxoglutarate-Dependent Oxygenases

: C–H functionalization is a chemically challenging but highly desirable transformation. 2-oxoglutarate-dependent oxygenases (2OGXs) are remarkably versatile biocatalysts for the activation of C–H bonds. In nature, they have been shown to accept both small and large molecules carrying out a plethora of reactions, including hydroxylations, demethylations, ring formations, rearrangements, desaturations, and halogenations, making them promising candidates for industrial manufacture. In this review, we describe the current status of 2OGX use in biocatalytic applications concentrating on 2OGX-catalyzed oxyfunctionalization of amino acids and synthesis of antibiotics. Looking forward, continued bioinformatic sourcing will help identify additional, practical useful members of this intriguing enzyme family, while enzyme engineering will pave the way to enhance 2OGX reactivity for non-native substrates.


Introduction
Alkanes are important, easily obtainable building blocks for the chemical industry. They stem from resources, such as gas and crude oil, but are also found in algae, bacteria, and plants and serve as precursors for a wide range of agrochemicals and pharmaceuticals. However, the activation of alkanes remains challenging in an industrial context due to the fact that carbon-hydrogen bonds are relatively inert. Additionally, when employing substrates with multiple C-H bonds, obtaining satisfactory chemo-, regio-and stereoselectivity remains problematic, while, at the same time, the oxidation state of the final product can be difficult to control using conventional chemical catalysis [1].
Nature, in contrast, has perfected oxidation reactions, which are an integral part of the biosynthesis of a diverse set of compounds, especially of secondary metabolites [2,3]. Nature's repertoire of oxidative biocatalysts can be mechanistically classified into two categories: dehydrogenation and oxyfunctionalization catalysts [4]. Dehydrogenation catalysts abstract a hydrogen atom via an acceptor molecule, such as a nicotinamide or flavin cofactor, but do not involve an active oxygen intermediate. Oxyfunctionalization catalysts, on the other hand, reductively activate molecular oxygen or rely on hydrogen peroxide for subsequent electrophilic substitution into the starting material. Synthetically, oxyfunctionalization catalysts are more intriguing, as they can introduce new functional groups by catalyzing the selective insertion of oxygen into C-H, C-C, and C=C bonds [4]. Harnessing the power of precisely placed cofactors in the enzyme active site, C-H activating biocatalysts derivatize their substrates regio-and stereospecifically at ambient temperature and pressure in aqueous solutions, thus eliminating the challenges typically associated with chemical approaches.  [29]; B) Halogenation of 12-epi-Fischerindole U by WelO5 to yield 12-epi-Fischerindole G [30]; C) Epoxidation of 4'-methoxyldehydroxy cyclopeptin by AsqJ to form 4'methoxylcyclopenin [31,32]; D) Oxidation of a tryptophan derivative by dioxygenase for auxinoxidation (DAO) to form the plant auxin indole-3-acetic acid [33][34][35]; E) O-demethylation of thebaine in morphinan alkaloid biosynthesis by codeine O-demethylase (CODM) [36]; F) Epimerization reaction in the carbapenem core structure maturation as catalyzed by CarC [37,38]; G) Ring expansion reaction of the penicillin N amide thiazolidine ring catalyzed by deacetoxycephalosporin C synthase (DAOCS) [39]; H, I) Ring formation and desaturation reaction in the biosynthesis of clavulanic acid catalyzed by trifunctional 2-oxoglutarate-dependent clavaminate synthase (CAS) [40]; J) Formation of an endoperoxide by FtmOx1 in verruculogen biosynthesis [41,42].
Structurally, 2OGX enzymes share distinctive features. Crystallographic studies show that the enzymes contain a double-stranded -helix core fold (DSBH, also known as a jelly roll, cupin, or jumonji C fold) composed of eight antiparallel -strands forming a -sandwich structure, which supports two His residues and (with exception of the halogenases) a carboxylate Glu or Asp residue [43]. This motif, also called the 2-His-1-carboxylate facial triad, is responsible for binding the Fe(II) center, whereas the other face of the metal coordinates three water molecules [44]. Aik et al. proposed that beyond housing the active site, the -strands also assist in providing selectivity for binding the primary substrates [45]. In the consensus view of the 2OGX reaction mechanism for hydroxylation activity (Figure 2), the most well-studied reactivity of 2OGX enzymes, two water molecules are displaced upon 2-oxoglutarate (2OG) chelation. 2OG binds to the Fe center in a bidentate configuration, in which the keto group is oriented opposite the Asp/Glu and its carboxylate opposite one of the His residues [46]. The binding of the primary substrate proximal to the metal center leads to the displacement of the remaining water molecule triggering the binding of dioxygen. In this way, the formation of the Fe(III)-superoxo species takes place, which initiates oxidative decarboxylation of 2OG to form succinate, CO2, and an Fe(IV) oxo  [29]; (B) Halogenation of 12-epi-Fischerindole U by WelO5 to yield 12-epi-Fischerindole G [30]; (C) Epoxidation of 4 -methoxyldehydroxy cyclopeptin by AsqJ to form 4 -methoxylcyclopenin [31,32]; (D) Oxidation of a tryptophan derivative by dioxygenase for auxinoxidation (DAO) to form the plant auxin indole-3-acetic acid [33][34][35]; (E) O-demethylation of thebaine in morphinan alkaloid biosynthesis by codeine O-demethylase (CODM) [36]; (F) Epimerization reaction in the carbapenem core structure maturation as catalyzed by CarC [37,38]; (G) Ring expansion reaction of the penicillin N amide thiazolidine ring catalyzed by deacetoxycephalosporin C synthase (DAOCS) [39]; (H,I) Ring formation and desaturation reaction in the biosynthesis of clavulanic acid catalyzed by trifunctional 2-oxoglutarate-dependent clavaminate synthase (CAS) [40]; (J) Formation of an endoperoxide by FtmOx1 in verruculogen biosynthesis [41,42].
Structurally, 2OGX enzymes share distinctive features. Crystallographic studies show that the enzymes contain a double-stranded β-helix core fold (DSBH, also known as a jelly roll, cupin, or jumonji C fold) composed of eight antiparallel β-strands forming a β-sandwich structure, which supports two His residues and (with exception of the halogenases) a carboxylate Glu or Asp residue [43]. This motif, also called the 2-His-1-carboxylate facial triad, is responsible for binding the Fe(II) center, whereas the other face of the metal coordinates three water molecules [44]. Aik et al. proposed that beyond housing the active site, the β-strands also assist in providing selectivity for binding the primary substrates [45]. In the consensus view of the 2OGX reaction mechanism for hydroxylation activity (Figure 2), the most well-studied reactivity of 2OGX enzymes, two water molecules are displaced upon 2-oxoglutarate (2OG) chelation. 2OG binds to the Fe center in a bidentate configuration, in which the keto group is oriented opposite the Asp/Glu and its carboxylate opposite one of the His residues [46]. The binding of the primary substrate proximal to the metal center leads to the displacement of the remaining water molecule triggering the binding of dioxygen. In this way, the formation of the Fe(III)-superoxo species takes place, which initiates oxidative decarboxylation of 2OG to form succinate, CO 2 , and an Fe(IV) oxo species, also known as the ferryl intermediate. This ferryl intermediate abstracts a hydrogen from an unactivated C(sp 3 )-H bond in the primary substrate yielding Fe(III)-OH and the substrate radical. Hydroxyl radical rebound yields the hydroxylated product, which dissociates to complete the catalytic cycle [24,47,48]. In cases where the carbon undergoing hydroxylation is bound to a heteroatom, the initially formed product may spontaneously react further [24]. species, also known as the ferryl intermediate. This ferryl intermediate abstracts a hydrogen from an unactivated C(sp 3 )-H bond in the primary substrate yielding Fe(III)-OH and the substrate radical. Hydroxyl radical rebound yields the hydroxylated product, which dissociates to complete the catalytic cycle [24,47,48]. In cases where the carbon undergoing hydroxylation is bound to a heteroatom, the initially formed product may spontaneously react further [24]. Notably, in 2OGX catalysis the electrons required for oxyfunctionalization of the unactivated C(sp 3 )-H bond stem from the cheap co-factor 2-oxoglutarate. In biocatalytic reactions, 2-oxoglutarate can be added from external sources; however, microbial metabolism may also provide this redox cofactor. The central carbon metabolism fuels 2OGX enzymes with 2-oxoglutarate and in turn assimilates and recycles the coproduct of the enzymatic reaction, namely succinate, through the tricarboxylic acid (TCA) cycle. Thus, metabolic engineering of microbial hosts to increase 2-oxoglutarate levels and force flux through the 2OGX biocatalyst is an attractive strategy to generate optimized cell factories. Using this approach, Theodosiou et al. and Smirnov et al. engineered the TCA cycle in Escherichia coli by deleting succinate-producing enzymes, thus optimizing 2OG flux for the production of trans-4-hydroxy-L-proline and trans-4-hydroxy-L-isoleucine, respectively [49,50]. Beyond their use as chassis strains for amino acid hydroxylation, these constructs also principally enable the selection of active 2OGX variants as the sole producers of succinate [49].
In this review, we will highlight the biocatalytic utility of 2OGX enzymes by outlining the applications of 2OGXs in the hydroxylation of amino acid derivatives and of antibiotics (for a comprehensive overview of halogenation reactions please refer to [51,52]). Future implications of the rapidly increasing number of available genomes, as well as the increasing sophistication of enzyme engineering approaches, will also be discussed. Notably, in 2OGX catalysis the electrons required for oxyfunctionalization of the unactivated C(sp 3 )-H bond stem from the cheap co-factor 2-oxoglutarate. In biocatalytic reactions, 2-oxoglutarate can be added from external sources; however, microbial metabolism may also provide this redox cofactor. The central carbon metabolism fuels 2OGX enzymes with 2-oxoglutarate and in turn assimilates and recycles the coproduct of the enzymatic reaction, namely succinate, through the tricarboxylic acid (TCA) cycle. Thus, metabolic engineering of microbial hosts to increase 2-oxoglutarate levels and force flux through the 2OGX biocatalyst is an attractive strategy to generate optimized cell factories. Using this approach, Theodosiou et al. and Smirnov et al. engineered the TCA cycle in Escherichia coli by deleting succinate-producing enzymes, thus optimizing 2OG flux for the production of trans-4-hydroxy-L-proline and trans-4-hydroxy-L-isoleucine, respectively [49,50]. Beyond their use as chassis strains for amino acid hydroxylation, these constructs also principally enable the selection of active 2OGX variants as the sole producers of succinate [49].
In this review, we will highlight the biocatalytic utility of 2OGX enzymes by outlining the applications of 2OGXs in the hydroxylation of amino acid derivatives and of antibiotics (for a comprehensive overview of halogenation reactions please refer to [51,52]). Future implications of the rapidly increasing number of available genomes, as well as the increasing sophistication of enzyme engineering approaches, will also be discussed.

Biocatalytic Applications of 2-oxoglutarate-Dependent Oxygenases
Amino acid hydroxylation is the most common industrial application of 2-oxoglutarate-dependent oxygenases to date ( Figure 3). Several filed patents attest to the enzyme family's potential for the biocatalytic production of derivatized amino acids, which serve as building blocks for the chemical industry [25,[53][54][55][56][57][58][59]. Beyond amino acid hydroxylation, 2-oxoglutarate-dependent oxygenases are explored for the synthesis of antibiotics, in the chemo-enzymatic total synthesis of complex natural products, and as part of enzymatic cascade reactions for the production of valuable building blocks.

Biocatalytic applications of 2-oxoglutarate-dependent oxygenases
Amino acid hydroxylation is the most common industrial application of 2-oxoglutarate-dependent oxygenases to date ( Figure 3). Several filed patents attest to the enzyme family's potential for the biocatalytic production of derivatized amino acids, which serve as building blocks for the chemical industry [25,[53][54][55][56][57][58][59]. Beyond amino acid hydroxylation, 2-oxoglutarate-dependent oxygenases are explored for the synthesis of antibiotics, in the chemo-enzymatic total synthesis of complex natural products, and as part of enzymatic cascade reactions for the production of valuable building blocks. Overview of selected amino acid hydroxylations by 2-oxoglutarate-dependent enzymes. The enzymes typically accept free-standing amino acids as a substrate. In cases where the substrate is part of a more complex molecule, the enzyme name is marked with an asterisk.

Aliphatic amino acid oxygenases
L-Isoleucine 4-hydroxylase from Bacillus thuringiensis converts L-isoleucine stereoselectively into (2S,3R,4S)-4-hydroxyisoleucine (4-HIL) [60], which was isolated for the first time from seeds of fenugreek (Trigonella foenum-graceum) [61]. The IDO-catalyzed conversion of L-isoleucine into 4-HIL ( Figure 4) is patented by the Japanese company Ajinomoto Co. Inc. [58] and the Chinese company Henan Julong Bio-Engineering Co. Ltd. [62]. Studies on the properties of the hydroxylation product associate 4-HIL with anti-diabetic effects [63] and show that it is an effective drug for body weight control, glycemia, and insulinemia [64]. In Bacillus thuringiensis, 4-HIL is further converted into (2S,3R)-2-amino-3-methyl-4-ketopentanoic acid, a vitamin B12 antimetabolite with antibiotic properties [65]. To boost 4-HIL production titers, an engineered E. coli strain lacking the isocitrate lyase and isocitrate dehydrogenase kinase/phosphatase was designed channeling endogenously produced co-substrate 2oxoglutarate toward the 2OGX [50]. Using this strain, 4-HIL yields were found to be 82%, employing 100 mmol L-isoleucine as a substrate [50]. Recently, Zhang et al. enhanced the activity of IDO by introducing four mutations, which were identified by error-prone PCR [66]. Using this approach, the The enzymes typically accept free-standing amino acids as a substrate. In cases where the substrate is part of a more complex molecule, the enzyme name is marked with an asterisk.

Aliphatic Amino Acid Oxygenases
L-isoleucine 4-hydroxylase from Bacillus thuringiensis converts L-isoleucine stereoselectively into (2S,3R,4S)-4-hydroxyisoleucine (4-HIL) [60], which was isolated for the first time from seeds of fenugreek (Trigonella foenum-graceum) [61]. The IDO-catalyzed conversion of L-isoleucine into 4-HIL ( Figure 4) is patented by the Japanese company Ajinomoto Co. Inc. [58] and the Chinese company Henan Julong Bio-Engineering Co. Ltd. [62]. Studies on the properties of the hydroxylation product associate 4-HIL with anti-diabetic effects [63] and show that it is an effective drug for body weight control, glycemia, and insulinemia [64]. In Bacillus thuringiensis, 4-HIL is further converted into (2S,3R)-2-amino-3-methyl-4-ketopentanoic acid, a vitamin B12 antimetabolite with antibiotic properties [65]. To boost 4-HIL production titers, an engineered E. coli strain lacking the isocitrate lyase and isocitrate dehydrogenase kinase/phosphatase was designed channeling endogenously produced co-substrate 2-oxoglutarate toward the 2OGX [50]. Using this strain, 4-HIL yields were found to be 82%, employing 100 mmol L-isoleucine as a substrate [50]. Recently, Zhang et al. enhanced the activity of IDO by introducing four mutations, which were identified by error-prone PCR [66]. Using this approach, the k cat /K m value of IDO could be increased 1.5-fold to reach approximately 33 L/mmol/min, and the 4-HIL production doubled in a small-scale biocatalysis reaction [66]. In a study to investigate IDO's substrate acceptance beyond L-isoleucine, recombinantly expressed IDO was successfully tested for the hydroxylation of the aliphatic amino acids L-leucin, L-norleucine, and L-norvaline and for the (S)-sulfoxidation of L-methionine and L-ethionine [67]. Exploiting this substrate promiscuity, the Kourist group established a stereoselective three-enzyme cascade for the synthesis of diasteromerically pure γ-oxyfunctionalized α-amino acids by combining IDO with an N-acylamino acid racemase (NAAAR) and an L-selective aminoacylase from Geobacillus thermoglucosidasius. In a one-pot strategy, 5 mM racemic N-acetylmethionine was quantitatively converted into L-methionine-(S)-sulfoxide with a 97% yield and 95% diastereomeric excess [68].
kcat/Km value of IDO could be increased 1.5-fold to reach approximately 33 L/mmol/min, and the 4-HIL production doubled in a small-scale biocatalysis reaction [66]. In a study to investigate IDO's substrate acceptance beyond L-isoleucine, recombinantly expressed IDO was successfully tested for the hydroxylation of the aliphatic amino acids L-leucin, L-norleucine, and L-norvaline and for the (S)sulfoxidation of L-methionine and L-ethionine [67]. Exploiting this substrate promiscuity, the Kourist group established a stereoselective three-enzyme cascade for the synthesis of diasteromerically pure γoxyfunctionalized α-amino acids by combining IDO with an N-acylamino acid racemase (NAAAR) and an L-selective aminoacylase from Geobacillus thermoglucosidasius. In a one-pot strategy, 5 mM racemic N-acetylmethionine was quantitatively converted into L-methionine-(S)-sulfoxide with a 97% yield and 95% diastereomeric excess [68].

L-Leucine 5-hydroxylase from Streptomyces
Manzacidins A-C, a family of bromopyrrole alkaloids with suspected biological activities, were first isolated from the Okinawan sponge Hymeniacidon sp. by Kobayashi et al. in 1991 [74,75]. Manzacidins possess a unique structure consisting of an ester-linked bromopyrrolecarboxylic acid and a 3,4,5,6-tetrahydropyrimidine ring in which one of the amino groups is attached to the C6 quaternary carbon center [75]. Several strategies for the total synthesis of manzacidin A and C were developed in the early years of the 21 st century, generating mg amounts of the final product [75]. In contrast to the purely chemical approach, Renata and co-workers investigated a chemo-enzymatic route to produce manzacidin C, harnessing the 2-oxoglutarate-dependent leucine 5-hydroxylase GriE from Streptomyces sp. DSM40835 [76]. GriE has been implicated in the biosynthesis of the anti-tuberculosis molecules To date, three L-leucine dioxygenases from various cyanobacteria have been investigated. The first enzyme, LdoA, was isolated from Nostoc punctiforme PCC73102 [71]. The characterization of its substrate acceptance highlighted the capability of this enzyme to hydroxylate L-leucine into (2S,4S)-5-hydroxyleucine and L-norleucine into (2S)-5-hydroxynorleucine and to carry out sulfoxidation of L-methionine and L-ethionine [71]. Recently, oxygenase NpLDO from Nostoc piscinale possessing 72% sequence identity to LdoA [72] and AvLDO from Anabaena variabilis [73] were analyzed. While the studies highlighted that both enzymes exhibit a similar substrate spectrum to LdoA, e.g., hydroxylation of L-isoleucine into (2S,4S)-5-hydroxyleucine and sulfoxidation of L-methionine, AvLDO shows a differing enantiopreference and forms L-methionine-(R)-sulfoxide in contrast to the (S)-enantiomer formed with L-isoleucine 4-hydroxylase (IDO).

L-leucine 5-hydroxylase from Streptomyces
Manzacidins A-C, a family of bromopyrrole alkaloids with suspected biological activities, were first isolated from the Okinawan sponge Hymeniacidon sp. by Kobayashi et al. in 1991 [74,75]. Manzacidins possess a unique structure consisting of an ester-linked bromopyrrolecarboxylic acid and a 3,4,5,6-tetrahydropyrimidine ring in which one of the amino groups is attached to the C6 quaternary carbon center [75]. Several strategies for the total synthesis of manzacidin A and C were developed in the early years of the 21 st century, generating mg amounts of the final product [75]. In contrast to the purely chemical approach, Renata and co-workers investigated a chemo-enzymatic route to produce manzacidin C, harnessing the 2-oxoglutarate-dependent leucine 5-hydroxylase GriE from Streptomyces sp. DSM40835 [76]. GriE has been implicated in the biosynthesis of the anti-tuberculosis molecules griselimycins found in Streptomyces sp. [77] by converting L-leucine into (2S,4R)-5-hydroxyleucine [78]. Investigations of GriE's substrate scope demonstrated the enzyme's ability to hydroxylate at least 13 amino acid derivatives with useful turnovers. While substitutions at the γ position of the amino acid analogs were generally accepted, the enzyme proved to be sensitive to substitution at the β position [76]. For the total chemo-enzymatic synthesis of manzacidin C ( Figure 5), photocatalytically produced azidoleucine (134 mg) was selectively δ-hydroxylated with 95% conversion using purified enzyme. Next, three additional chemical steps yielded the final product manzacidin C with complete stereocontrol shortening the known chemical routes substantially [76]. In an alternative set-up, the same group highlighted the possibility of generating manzacidin C via the gram scale hydroxylation of L-leucine by whole cell biocatalysis. The total synthesis consisting of nine steps led to the desired product in 11% total yield from L-leucine [79].
griselimycins found in Streptomyces sp. [77] by converting L-leucine into (2S,4R)-5-hydroxyleucine [78]. Investigations of GriE's substrate scope demonstrated the enzyme's ability to hydroxylate at least 13 amino acid derivatives with useful turnovers. While substitutions at the γ position of the amino acid analogs were generally accepted, the enzyme proved to be sensitive to substitution at the β position [76]. For the total chemo-enzymatic synthesis of manzacidin C ( Figure 5), photocatalytically produced azidoleucine (134 mg) was selectively -hydroxylated with 95% conversion using purified enzyme. Next, three additional chemical steps yielded the final product manzacidin C with complete stereocontrol shortening the known chemical routes substantially [76]. In an alternative set-up, the same group highlighted the possibility of generating manzacidin C via the gram scale hydroxylation of Lleucine by whole cell biocatalysis. The total synthesis consisting of nine steps led to the desired product in 11% total yield from L-leucine [79].
In elevated concentrations, GriE can convert L-leucine into the corresponding imine by double oxidation at C5. The resulting imine can be reduced to (2S,4R)-4-methylproline [76], an important building block for the synthesis of depsipeptide antibiotics or the antifungal agent cavinafungin B [80]. Cavinafungin B, an aldehyde lipopeptide isolated from Colispora cavincola in 2015 [81], is a close analogue to cavinafungin A, which was recently shown to possess antiviral properties against the Dengue virus and the Zika virus [82]. The total synthesis of cavinafungin B (37% final yield) was carried out via a 10-step Fmoc-based solid-phase peptide synthesis (SPPS) using chemo-enzymatically generated (2S,4R)-4-methylproline as a key building block ( Figure 5) [80].

Dioxygenase for Auxinoxidation and Fumitremorgin Oxygenases
Tryptophan or indole-derived natural products are common in nature, and the literature provides evidence that 2-oxoglutarate-dependent enzymes are involved in their biosynthesis. In plants, for example, the dioxygenase for auxinoxidation (DAO) from Arabidopsis hydroxylates the natural product auxin indole-3-acetic acid into the inactive 2-oxoindole-3-acetic acid [33][34][35], while the microbial FtmOx1 from Aspergillus fumigatus catalyzes the conversion of fumitremorgin B into the mycotoxin verruculogen. FtmOx1 operates by inserting an endoperoxide bond between two prenyl moieties [41,42]. This enzymatic activity is dependent on a specific residue, Tyr 244, which has been identified by mechanistic and structural studies [88,89]. However, despite their interesting activities, these enzymes have not yet featured in biocatalytic applications.

L-proline Hydroxylases
In nature, hydroxyprolines are the building blocks of small molecules, such as actinomycin 1 or etamycin, but also occur as part of proteins. A class of 2-oxoglutarate-dependent oxygenases named proline hydroxylases (PHs) can catalyze the conversion of L-proline either into 3-hydroxy-L-proline or 4-hydroxy-L-proline [51]. The first described PH was isolated from Streptomyces griseoviridus P8648 (SgP4H) in 1994. It selectively epoxidizes 3,4-dehydro-L-proline, yielding trans-3,4-epoxy-L-proline [90]. Since then, a number of additional PHs have been discovered and characterized, and today, all four monohydroxy-isomers of L-proline can be produced enzymatically (Table 1). However, it should be noted that trans-3-hydroxy-L-proline is only formed as a side product by the enzymes HtyE and GloF, two enzymes, which are known to preferentially produce trans-4-hydroxyproline [91].  The company Kyowa Hakko Kogyo has patented several PH-based processes. In an initial patent, the company protects the use of a 2-oxoglutarate-dependent oxygenase, namely cis-P3H1, for the production of cis-3-hydroxy-L-proline [55]. They describe the use of the purified enzyme to convert L-proline (20 mM) into cis-3-hydroxy-L-proline (18mM) in 30 min. Additional patents of the same company cover the production of cis-4-hydroxy-L-proline using SmP4H [56] and the manufacture of trans-4-hydroxy-L-proline with DaP4H [54]. In the latter case, strain optimization took place, and by expressing DaP4H with a tryptophan tandem promotor, the desired product trans-4-hydroxy-L-proline could be obtained in titers of up to 41 g/L (87% yield) in 100 h [101]. Beyond Kyowa Hakko, the Japanese company API Corp holds a patent for the production of cis-5-hydroxypipecolic acid using a proline hydroxylase from Xenorhabdus sp. [53].
Other proline hydroxylases also accept the industrially important structural homolog pipecolic acid. Klein et al. used Cis-P3H1, SmP4H, and DaP4H for the synthesis of hydroxypipecolic acid isomers [105]. To improve SmP4H's regioselectivity in the conversion of pipecolic acid, Codexis Inc. optimized the enzyme by protein engineering, yielding a variant capable of a more selective conversion of L-pipecolic acid into cis-5-hydroxypipecolic acid [25,57]. Interestingly, the fungal enzymes GetF and PiFa preferentially accept pipecolic acid, whereas L-proline is converted only to a very small extent. Using site-directed mutagenesis, the group of Hüttel attempted to shift enzyme activity towards L-proline, albeit with little success [95].
In some cases, the biocatalytic use of proline hydroxylases can be hampered by their low temperature stability. In a recent study, Liu et al. addressed this problem for enzyme UbP4H by loop grafting and site-directed mutagenesis improving T m from 43 • C to 46 • C [99].

L-Hydroxyasparagine Oxygenases
Calcium-dependent antibiotics (CDAs) are structurally related acidic lipopeptide antibiotics, which possess a decapeptide lactone or a lactam motif containing non-proteinogenic amino acids and feature N-terminal fatty acid side chains [106]. The most prominent representative of this antibiotic class is daptomycin [107]. L-3-hydroxy-asparagine is an important building block for the biosynthesis of CDAs in Streptomyces coelicolor [106]. The hydroxylation of L-asparagine is catalyzed by asparagine oxygenase (AsnO) [108]. By solving the crystal structure of AsnO with a resolution of 1.45 Å, Striecker and coworkers demonstrated that only free L-asparagine can be converted by the enzyme, whereas the late-stage functionalization of CDA or the acceptance of an asparagine bound to a peptidyl carrier protein was excluded [109]. Using protein engineering, the group succeeded in switching substrate acceptance to L-aspartic acid. Upon the introduction of only one mutation (D241N), the enzyme produced L-3-hydroxy-aspartic acid exclusively, albeit with a loss of catalytic efficiency (the wildtype has a 300-time higher k cat ) [110]. The preparative synthesis of L-3-hydroxy-aspartic acid was also demonstrated on the scale of 18 mg with a purified protein [110].
In a biocatalytic context, VioC has been used together with an arginase and an ornithine cyclodeaminase to convert L-arginine into trans-3-hydroxy-L-proline, which is difficult to selectively produce using proline hydroxylases [117]. Despite successfully setting up the enzymatic cascade, Hara et al. concluded that long reaction times (72 h) and low ornithine cyclodeaminase activity make industrial applications unlikely at this time [117].
The protected building block tambroline can be synthesized by selective C3 hydroxylation of L-lysine, employing the 2-OXG lysine hydroxylase KDO1 from Catenulispora acidiphila (DSM 44928) [120,121] followed by an additional four chemical steps [122]. The biocatalytic transformation was established on a 4 g scale (35 mM) using a crude cell lysate of KDO1 (>99% conversion) [122]. The chemo-enzymatic total synthesis of tambromycin consisted of 10 steps and led to the desired product with an overall yield of 2.4%. By comparison, in a recently published purely chemical synthesis consisting of 13 steps, tambromycin was obtained with an overall yield of 1.3% [123], positively underscoring the potential of chemo-enzymatic setups.

L-Glutamate Hydroxylase
Two 2-oxoglutarate-dependent enzymes, namely KtzO and KtzP, are involved in the natural synthesis of the antifungal and antimicrobial kutznerides [125]. In this specific case, the substrate L-glutamate is bound to a peptidyl carrier to enable conversion to threo-L-hydroxyglutamic acid by KtzO or erythro-L-hydroxyglutamic acid by KtzP [125]. Both enzymes cannot convert free-standing L-glutamate.

Deacetoxycephalosporin C synthase (DAOCS) and deacetylcephalosporin C synthase (DACS)
Cephalosporins are a class of beta-lactam antibiotics originally derived from the fungus Acremonium [134]. The ring expansion of the penicillin N amide thiazolidine ring to form the dihydrothiazine caphem ring, a key step in the biosynthesis of cephalosporins, is catalyzed by the 2oxoglutarate-dependent eacetoxycephalosporin C synthase (DAOCS) before the second 2-oxoglutaratedependent deacetylcephalosporin C synthase (DACS) hydroxylates at C-3' position to form deacetylcephalosporin C (Figure 7) [39]. In eukaryotic microorganisms, a single bifunctional enzyme called DAOCS/DACS catalyzes both reactions [135], whereas in prokaryotes two distinct enzymes are needed [136,137]. Industrially, penicillin G is a more abundantly available starting material than penicillin N, which is the natural substrate of DAOCS. However, wildtype DAOCS accepts penicillin G poorly, a fact that triggered a number of enzyme engineering studies to improve the activity of DAOCS for this nonnatural substrate [28,[138][139][140]. Single mutations have already had a profound effect on DAOCS activity; for example, mutation N304K increases the kcat /Km value for the conversion of penicillin G from 18 M −1 s −1 to 256 M −1 s −1 [141]. In a different study, error-prone PCR yielded a quadruple DAOCS mutant (C155Y/Y184H/V275I/C281Y) with a 40-fold improvement in kcat /Km [142], while further engineering generated a variant with a 118-fold improved kcat /Km value compared with the wildtype [143].

Deacetoxycephalosporin C Synthase (DAOCS) and Deacetylcephalosporin C Synthase (DACS)
Cephalosporins are a class of beta-lactam antibiotics originally derived from the fungus Acremonium [134]. The ring expansion of the penicillin N amide thiazolidine ring to form the dihydrothiazine caphem ring, a key step in the biosynthesis of cephalosporins, is catalyzed by the 2-oxoglutarate-dependent eacetoxycephalosporin C synthase (DAOCS) before the second 2-oxoglutarate-dependent deacetylcephalosporin C synthase (DACS) hydroxylates at C-3 position to form deacetylcephalosporin C (Figure 7) [39]. In eukaryotic microorganisms, a single bifunctional enzyme called DAOCS/DACS catalyzes both reactions [135], whereas in prokaryotes two distinct enzymes are needed [136,137]. Figure 6. Biosynthesis of clavulanic acid. The tri-functional oxygenase CAS sequentially carries out a hydroxylation, ring formation, and desaturation step.

Deacetoxycephalosporin C synthase (DAOCS) and deacetylcephalosporin C synthase (DACS)
Cephalosporins are a class of beta-lactam antibiotics originally derived from the fungus Acremonium [134]. The ring expansion of the penicillin N amide thiazolidine ring to form the dihydrothiazine caphem ring, a key step in the biosynthesis of cephalosporins, is catalyzed by the 2oxoglutarate-dependent eacetoxycephalosporin C synthase (DAOCS) before the second 2-oxoglutaratedependent deacetylcephalosporin C synthase (DACS) hydroxylates at C-3' position to form deacetylcephalosporin C (Figure 7) [39]. In eukaryotic microorganisms, a single bifunctional enzyme called DAOCS/DACS catalyzes both reactions [135], whereas in prokaryotes two distinct enzymes are needed [136,137]. Industrially, penicillin G is a more abundantly available starting material than penicillin N, which is the natural substrate of DAOCS. However, wildtype DAOCS accepts penicillin G poorly, a fact that triggered a number of enzyme engineering studies to improve the activity of DAOCS for this nonnatural substrate [28,[138][139][140]. Single mutations have already had a profound effect on DAOCS activity; for example, mutation N304K increases the kcat /Km value for the conversion of penicillin G from 18 M −1 s −1 to 256 M −1 s −1 [141]. In a different study, error-prone PCR yielded a quadruple DAOCS mutant (C155Y/Y184H/V275I/C281Y) with a 40-fold improvement in kcat /Km [142], while further engineering generated a variant with a 118-fold improved kcat /Km value compared with the wildtype [143]. Industrially, penicillin G is a more abundantly available starting material than penicillin N, which is the natural substrate of DAOCS. However, wildtype DAOCS accepts penicillin G poorly, a fact that triggered a number of enzyme engineering studies to improve the activity of DAOCS for this non-natural substrate [28,[138][139][140]. Single mutations have already had a profound effect on DAOCS activity; for example, mutation N304K increases the k cat /K m value for the conversion of penicillin G from 18 M −1 s −1 to 256 M −1 s −1 [141]. In a different study, error-prone PCR yielded a quadruple DAOCS mutant (C155Y/Y184H/V275I/C281Y) with a 40-fold improvement in k cat /K m [142], while further engineering generated a variant with a 118-fold improved k cat /K m value compared with the wildtype [143].

Conclusions
Due to the explosion of available genome information, bioinformatic studies continuously identify additional members of the 2OGX superfamily in bacteria, metazoa, and plants [144][145][146]. This wealth of available enzyme sequences will lead to the characterization of additional, industrially promising 2OGX variants with desirable substrate spectra. Already today, 2-oxoglutarate-dependent oxygenases are employed for a variety of industrially and academically relevant transformations, ranging from the production of amino acid derivatives to the chemo-enzymatic total synthesis of complex natural products (vide supra). As attested by recent enzyme engineering studies on 2-oxoglutarate-dependent oxygenases [25,99,140], the progress made in the field of protein design and evolution over the last few decades [147,148] facilitates the development of robust and active C-H functionalization biocatalysts [149]. As the integration of automation and the use of advanced computer algorithms continue to facilitate enzyme engineering, the industrial implementation of 2OGX-driven processes will be further accelerated.