Industrial Applications of Enzymes : Recent Advances , Techniques , and Outlooks

Enzymes as industrial biocatalysts offer numerous advantages over traditional chemical processes with respect to sustainability and process efficiency. Enzyme catalysis has been scaled up for commercial processes in the pharmaceutical, food and beverage industries, although further enhancements in stability and biocatalyst functionality are required for optimal biocatalytic processes in the energy sector for biofuel production and in natural gas conversion. The technical barriers associated with the implementation of immobilized enzymes suggest that a multidisciplinary approach is necessary for the development of immobilized biocatalysts applicable in such industrial-scale processes. Specifically, the overlap of technical expertise in enzyme immobilization, protein and process engineering will define the next generation of immobilized biocatalysts and the successful scale-up of their induced processes. This review discusses how biocatalysis has been successfully deployed, how enzyme immobilization can improve industrial processes, as well as focuses on the analysis tools critical for the multi-scale implementation of enzyme immobilization for increased product yield at maximum market profitability and minimum logistical burden on the environment and user.

This review focuses on how enzyme catalysis has been advantageously used in chemical processes and which industries can further exploit enzyme catalysis for improved outcomes.
Further, this review contains an in-depth discussion of the latest enzyme immobilization techniques, how enzyme immobilization can aid in the realization of fully optimized biocatalysts, and the combination of technical expertise that will drive the scale-up of these economically competitive immobilized-biocatalytic processes for industrial applications.

Enzyme Implementation: A Societal Need
The pharmaceutical, food and beverage, detergent, and biofuel industries have reaped the advantages of enzyme catalysis in commercial-scale applications, while other industries, such as natural gas conversion and fine chemical production, are only recently considering their use [1][2][3][4].In industrial-scale chemical production, the benefits of biocatalysis are often multifaceted, and as such, enzymes are attractive catalysts owing to mild reaction conditions, high product selectivity, and low environmental impact, and thus have been employed for both simplified chemical synthesis routes and improved chemical process economics [1,3,5]; Table 1 illustrates the broad applications of enzyme catalysis throughout various industries.

Enzyme Immobilization for Expanded Scope of Implementation
Studies in enzyme immobilization, i.e., the attachment of the biocatalyst to a material with desired physical, chemical, electrical, or mechanical properties, have shown that immobilizing biocatalysts can improve their activity and stability across a broader range of operating conditions, with the additional functionality being imparted depending upon both the method of immobilization as well as the inherent properties of the materials used in such immobilization [49,[62][63][64][65][66].It was further demonstrated that the immobilized biocatalysts are novel in that their application simultaneously also allows for a reduced number of processing steps due to the facile separation of the biocatalyst itself from its reaction mixture, their retention of catalytic activity, and the resulting appreciable degree of reusability [5,[20][21][22][23][24][25][26][27].Table 2 lists both the common advantages and disadvantages associated with the use of an immobilized biocatalyst, as highlighted in previous research.
Three main immobilization techniques (Figure 2) have been largely reported in the literature, namely carrier-bound attachment, encapsulation or entrapment, and the formation of crosslinked enzyme aggregates [20][21][22][23][24][25][26][27].Two kinetic parameters are often calculated for an immobilized enzyme to assess the effects of immobilization on the enzyme's catalytic efficiency when compared to the non-immobilized enzyme counterpart, i.e., the Michaelis constant K m and maximal reaction velocity V max .K m compares the rates of substrate-enzyme binding and dissociation with smaller values of K m suggesting that binding dominates and indicating higher enzyme-substrate affinity [1].V max measures the rate at which an enzyme converts the substrate to product, and when controlled for catalytic mass, the value of V max is an appropriate measure of catalytic activity [1].
Figure 2. Schematic of different enzyme immobilization techniques.The crystal structure of Glucose Oxidase (GOx) isolated from Aspergillus Niger was used as a model enzyme (PDB ID: 3QVP) [67].Physical and covalent immobilization techniques are discussed relative to flat nanosupport graphene and curved nanosupport, carbon nanotube respectively.Encapsulation is discussed relative to a pore geometry higher than the diameter of the enzyme, while cross-linking is illustrated relative to the distance between two individual GOx.

Carrier-Bound Enzyme Immobilization through Both Physical and Chemical Binding
Carrier-bound enzyme immobilization is characterized by the attachment of the biocatalyst onto a prefabricated solid material with the appropriate immobilization methods being selected to allow for optimization of the catalytic performance [5,68].The two common methods of carrier-bound enzyme immobilization are physisorption and chemisorption, with physical adsorption offering the benefit of a generally universal, facile immobilization method, since the binding mechanism is not dependent on a site-specific chemical reaction between the enzyme and the support [49], while the covalent bonding requires site-specific chemical interactions between the enzyme and the support or the use of a cross-linking reagent [17].
Even though a wide range of both organic and inorganic supports, including ceramics and metal oxides [69,70], nanomaterials [68,[71][72][73][74][75], and polymers [76][77][78][79][80][81][82][83][84] have been investigated as supports for enzyme immobilization [24], the application of such physically adsorbed enzyme-support conjugates is limited by enzyme leaching as well as a decrease in the enzyme's catalytic efficiency [24].Falus et al., for instance, reported the immobilization of subtilisin A onto various silica gels for the continuous production of racemic N-Boc-phenylalanine ethyl thioester, an important pharmaceutical intermediate [83].Subtilisin A was physisorbed to surface-grafted silica gel and used as packing in three reactors in series for the dynamic kinetic resolution of racemic N-Boc-phenylalanine ethyl thioester.At optimal conditions, the continuous flow process yielded a 97% conversion of the substrate at an enantiomeric excess of 99.5%, with the immobilized subtilisin A retaining catalytic activity after 120 h in continuous flow operation.The improved activity retention and shelf life was reported to be up to 1 year and was attributed to the increased thermostability of the enzyme upon physisorption [83].
Burkholderia sp.lipase, an enzyme widely studied for the production of biodiesel, was immobilized onto magnetic nanoparticles and evaluated for its catalytic activity.Tran et al. found that methyl-grafted Fe 3 O 4 -SiO 2 nanocomposites had a high affinity for lipase (29.5 mg lipase g −1 nanocomposite being adsorbed) most likely due to the porous structure of the silica coating.A higher K m value and lower V max value were however reported for the immobilized enzyme, thus indicating that the immobilization process decreased both the catalytic activity and its efficiency, likely due to the non-specific attachment and deformation of the enzyme active site and its increased mass transfer resistance.The immobilization also allowed for the improved reusability and separation of lipase in the transesterification of olive oil with methanol to produce fatty acid methyl esters (FAMEs).Physisorption of lipase onto magnetic methyl-grafted Fe 3 O 4 -SiO 2 nanoparticles was shown to retain significant activity for up to 10 reaction cycles owing to its increased stability from multi-point hydrophobic interactions with grafted methyl groups [70].
Zhang et al. reported on the immobilization of catalase onto carbon nanotubes for application in nanoelectronics, biosensing, and high-resolution imaging.Carbon nanotubes have been extensively studied as supports for enzymes due to their high surface area-to-volume ratio and biocompatibility [72].An optimal enzyme loading (1.88 mg m −2 ) was found for the physisorption of catalase onto oxidized single wall nanotubes (O-SWNT).A K m value for O-SWNT-catalase conjugates, relative to that of the free enzyme, was reported to be 27.0%,indicating that the adsorptive interactions induced conformational changes in the secondary structure of the enzyme, as confirmed by Fourier transformation infrared spectroscopy and circular dichroism (CD) analyses.V max for O-SWNT-catalase conjugates was reported to be 6.3 times lower than that of free enzyme.An analysis of CD spectra for immobilized catalase suggested that hydrogen bonding between enzyme and O-SWNT caused increased enzyme rigidity and therefore increased activity retention [72].Lastly, Nidetzky's group has shown that chimeras of target enzymes can be combined with silica binding modules (SBM) through noncovalent interaction and become very tightly attached to such underivatized glass, even at physiological pH conditions.Moreover, the research has shown that the immobilized enzymes displayed full biological activity, suggesting that their binding to such a glass surface could be controlled through their specific orientation at the SBM interface [85,86].
Immobilization via covalent attachment was shown to offer strong chemical bonding that prevents significant enzyme leaching and further mitigates the loss of enzyme active sites [1].Covalent binding methods are however more intensive and chemically harsher than physical adsorption, often requiring activation steps capable of inducing enzyme denaturation [87].Further, the selection of an enzyme to be covalently immobilized must be carefully evaluated to ensure optimal catalytic efficiency, and as such, the enzyme-support covalent bond, for instance, should not affect the amino acids associated with the enzyme active site, or the immobilization method may cause loss of catalytic activity [87].
Zhu and Sun successfully immobilized lipase from Candida rugosa onto poly(vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibrous membranes via glutaraldehyde activation for hydrolysis of p-nitrophenyl palmitate [75].It was determined that covalent bonding caused an increase in K m and a decrease in V max due to slower substrate diffusion and decreased enzyme mobility at the interface.Immobilized lipase was also found to retain nearly 90% of its activity after incubation in a phosphate buffer system at 55 • C for 75 min, while free enzymes were found to retain only approximately 20% of their initial activity.Significantly more activity than free lipase was also retained after 30 days of storage at 4 • C most likely due to a decrease in denaturation [75].
Kuo et al. reported on the immobilization of the same enzyme for the synthesis of 2-phenylethyl acetate, the major aromatic ester of rose fragrance.In this study, lipase was covalently bonded to polyvinylidene fluoride (PVDF) membrane, activated via 1,4-diaminobutane and glutaraldehyde, resulting in an enzyme loading of 1.71 mg enzyme g −1 PVDF.The immobilization technique also led to improved catalytic activity with only slightly hindered catalytic efficiency in n-hexane, likely due to the preservation of the tertiary structure in the organic medium resulting from covalent immobilization [77].Complementarily, a study by Mendes et al. demonstrated that the optimal immobilization protocol among carrier-binding methods for lipase from Penicillium camembertii is covalent attachment to an epoxy-silica-polyvinyl alcohol composite.The covalently bound lipase was found to have a lower, less variable enzyme loading capacity than the physically adsorbed lipase.Moreover, the optimal case for covalently bound lipase yielded a hydrolytic activity nearly double that of physical adsorption as well as a greater activity retention.Covalent attachment of lipase to epoxy-silica-polyvinyl alcohol also resulted in improved thermostability compared to that of free lipase [88].
Epoxide hydrolase (EH) has also been studied for its potential application in the synthesis of high-value, enantiomerically pure pharmaceutical intermediates and other bioactive molecules.Petri et al., for instance, proposed the covalent attachment of EH from Aspergillus niger to epoxide-activated silica gel for the enantioselective hydrolysis of p-nitrostyrene oxide.Immobilization onto the silica gel resulted in a relatively high immobilization yield of nearly 70%, and the immobilized EH was found to retain about 90% of its activity relative to free EH, as well as good storage stability over the span of few months.The covalent immobilization of EH caused no decrease in the enantiomeric selectivity of p-nitrostyrene oxide hydrolysis and markedly improved the stability of EH in organic solvent of 20% DMSO [89].
Nanomaterials have been studied as supports for biocatalysts due to minimal mass transport limitations and high specific surface area for volume-efficient catalysis [22,68].Li et al., for instance, used an electrospun polyacrylonitrile-glycopolymer nanofibrous membrane as a support for covalent binding of catalase from bovine liver.Immobilized catalase activity was about 50% of that of the free catalase, but was found to be stable across broader ranges of temperatures and pHs.It was also found that covalently immobilized catalase retained approximately 80% relative activity after storage at 4 • C for 30 days, whereas free catalase retained no relative activity when in the same conditions [68].Alptekin et al. optimized a protocol of the chemical attachment of catalase onto Eupergit C, a macroporous derivative of methacrylamide reported to be chemically and mechanically stable as a catalyst for operation in batch and plug flow reactors.The ratio of K cat to K m was calculated to assess the catalytic efficiency of free and immobilized catalase and was found to be nearly 2 orders of magnitude, thus suggesting that the immobilized enzyme was less efficient in converting the substrate to product.However, immobilization was shown to improve enzyme shelf life and operational stability as a biocatalyst in batch and plug flow reactors.Studies showed that immobilized catalase retained nearly 78% of initial activity when measured 28 days after immobilization, whereas free catalase was inactive after only 11 days of storage.Furthermore, immobilized catalase retained 50% activity at 82 min in a plug flow reactor [90].

Enzyme Entrapment
Enzyme entrapment is the immobilization of a biocatalyst into carriers of varying degrees of porosity and permeability [27].Enzymes immobilized via entrapment exhibit improved stability due to intensified control of their microenvironment and were also shown to be more catalytically active at higher temperatures in organic solvents, as well as easily separated from substrate-product reaction mixture [87].
Immobilization via entrapment in a variety of carriers, e.g., sol gels, hydrogels, polymers, nanomaterials, has been researched for the employment of biocatalysts in the synthesis of organic compounds and for novel biosensing systems [64,66].Complementarily, the immobilization of lipase has been proposed for application in the production of flavor and fragrance chemicals, as well.Ferraz et al., for instance, investigated the viability of geranyl propionate synthesis using lipase from Penicillium crustosum as biocatalyst.Lipase was entrapped in beads nearly 0.5 cm in diameter via a crosslinking reaction between calcium chloride and sodium alginate.Calcium-alginate beads containing lipase were optimized further for geraniol and propionate conversion, as well as tested for reusability.Results show that the activity retention of immobilized lipase decreased linearly with respect to the number of cycles of use, suggesting that activity loss was due to enzyme leaching during each cycle [91].Risso et al. studied the same entrapment method for the immobilization of inulinase from Kluyveromyces marxianus, an important biocatalyst in the production of high fructose syrups.Inulinase, entrapped in calcium-alginate beads, was characterized by the determination of its kinetic parameters, as well as its thermostability and pH stability in varying degrees of organic solvents.The K m value of immobilized inulinase was found to be significantly less than that of free inulinase at optimal mass fractions of organic solvent, while the V max value of immobilized inulinase was comparable to that of free inulinase in the same conditions.However, mass transfer resistances, which would likely be the rate-limiting process, were not considered in the kinetic analysis of the immobilized biocatalyst [92].
Arica et al. proposed the entrapment of catalase from bovine liver in thermally reversible cylinders of poly(isopropylacrylamide-co-hydroxyethylmethacrylate) for reactor system applications.Immobilized catalase exhibited a decrease in catalytic activity and enzyme-substrate affinity, and retained less activity at higher temperatures than free catalase.It was also found that an increase in temperature caused for a decrease in hydrogel swelling and higher mass transfer resistance.The apparent kinetic parameters of the immobilized catalase were largely attributed to the temperature-dependent behavior of the hydrogel carrier itself.The entrapment technique allowed for enzyme reusability and increased storage stability.Immobilized catalase also showed 78% activity retention after storage at 4 • C for 20 days, while free catalase retained none of its activity in the same storage conditions.Furthermore, hydrogel-entrapped catalase was found to retain approximately 95% activity for 6 cycles in the batch reactor system [93].
Singh et al. studied the apparent kinetic and stabilizing effects of the encapsulation of bovine liver catalase in hollow silica nanoparticles (HSNPs).No absorption peaks were observed for catalase or hydrogen peroxide in the supernatant liquid isolated from the immobilization procedure, thus indicating an immobilization yield of nearly 100%.It was further determined that the encapsulation technique decreased both enzyme's activity and enzyme-substrate affinity.However, immobilized catalase showed significantly improved stability throughout a broad range of pHs and temperature conditions.Free catalase was completely denatured when tested for activity at 70 • C, while encapsulated catalase was found to have optimal catalytic activity at 80 • C. The encapsulation of catalase within HSNPs-rather than the physical adsorption of catalase onto HSNPs-was demonstrated by the thermostability results for immobilized enzyme.It is expected that physically adsorbed enzyme would show a loss of catalytic activity near the denaturation temperature of free enzyme [94].
Yan et al. reported the successful nanogel encapsulation of bovine carbonic anhydrase (BCA), a metalloenzyme that is studied for applications in carbon capture and biocatalytic enrichment of natural gas where industrial application is limited by the almost total loss of enzyme catalytic activity at 63 • C due to the irreversible aggregation of BCA.Acryloylation and subsequent in-situ polymerization of BCA to form single BCA nanogels were performed, thus imparting molecular structural stability to the enzyme while mitigating mass transfer limitations.BCA nanogels exhibited similar catalytic activity as free BCA and showed significant retention of activity at temperatures greater than 63 • C. It was also determined that nanogel encapsulation preserved the secondary structure of BCA, therefore inhibiting irreversible aggregation and allowing for catalytic activity even at 81 • C [95].
Enzyme entrapment in biocompatible nanoparticles and solid supports has also been reported as a novel approach for the improvement of enzyme activity as a result of biocatalyst-carrier interactions.Studies of enzyme entrapment in solid carriers have shown that for optimal immobilization conditions it is possible to "lock" immobilized enzymes into more catalytically active conformations [96].Prakasham et al., for instance, investigated the kinetic parameters and stability of amylase entrapped in matrices comprised of nickel-impregnated silica paramagnetic particles.It was observed that the entrapped amylase had more rapid starch hydrolysis than the free amylase, for all the tested pHs and temperature conditions.A lower K m value was however recorded for the immobilized amylase, most probably indicating that the entrapment technique yielded a more efficient, robust biocatalyst [96].
Wu et al. reported on the facile co-immobilization of enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP) into a metal-organic framework.The entrapment was performed by mixing solutions of zinc nitrate, GOx, and HRP, and 2-methylimidazole at ambient conditions for 0.5 h resulted in the enzyme-embedded zeolitic imidazolate framework (GOx&HRP/ZIF-8).The catalytic activity of such conjugate was compared to that of a mixture of GOx/ZIF-8 and HRP/ZIF-8 to determine any changes in efficiency as resulted from the co-immobilization technique.Analysis showed that GOx&HRP/ZIF-8 exhibited a 2 times higher activity than the mixture of single-immobilized conjugates due to a significant decrease in mass transfer resistance.Furthermore, GOx&HRP/ZIF-8 were found to retain significantly more activity than free enzyme in organic solvent and when stored at room temperature [97].Currently however, the successful scale-up of entrapped enzymes for biocatalysis is prevented by mass transfer limitations of substrate through carrier material, enzyme leaching, and low total catalytic mass of enzyme-carrier conjugate [24].Lastly, Lin et al. reported on the entrapment of HRP in inorganic interfaces made with cooper phosphate supports and in aqueous solution.Results showed that the hierarchical flower-like spherical structures considerably enhanced enzyme's activity relative to that of the free enzyme in solution.In addition, the hybrid interfaces also exhibited excellent reusability and reproducibility even when several cycles for evaluating the active hydrogen peroxide (H 2 O 2 ) release were performed [98].
CLEAs were shown to offer the benefits of enhanced shelf life and operational stability, reusability, and exceptional resistance to the leaching of immobilized biocatalyst in aqueous media, while not suffering from substrate diffusion limitations that could potentially reduce catalytic activity [100].In certain instances, CLEAs were shown to possess higher catalytic activities than the corresponding free enzymes, and this phenomenon, known as hyperactivation, was attributed to the aggregation of enzyme in a pre-organized tertiary structure that rendered it permanently insoluble upon cross-linking [24].Thus, CLEAs showed a large potential for application in industrial-scale processes owing to high catalytic productivity and inexpensive immobilization methods [24].However, the successful scale-up of applications were dependent on improving CLEA's mechanical properties while better defining separation criteria for continuous processes [24].Specifically, Lai et al. reported the direct formation (from fermentation broth) and stability analysis of CLEAs with lipase from Penicillium expansum (PEL) in various solvents, for the production of biodiesel from corn and microalgal oil, respectively.In this study, PEL-CLEAs were found to be less catalytically active than free PEL, likely due to mass transfer limitations of the large substrate molecules.PEL-CLEAs also showed an improved stability over free PEL at increased temperatures and in various conditions of pH.The clumping of PEL-CLEAs and loss of enzyme active sites was however determined to cause a decrease in the yield of biodiesel.PEL-CLEAs also exhibited substantial activity retention in nonaqueous solutions, suggesting that the immobilization method can be geared toward industrial production of biodiesel [106].
Nguyen and Yang produced combined cross-linked enzyme aggregates (combi-CLEAs) of GOx and HRP for the catalysis of a cascade chemical reaction applicable to glucose detection biosystems [105] and pharmaceutical wastewater treatment [109].The combi-CLEAs were optimized for the cross-linking density and mass ratio of GOx to HRP for ensuring maximal catalytic activity and enzyme stability.Upon optimization, combi-CLEAs showed similar catalytic activity relative to free enzymes, but lower values of K m were most likely a result of two factors: the distance of mass transfer for hydrogen peroxide intermediate, which was substantially reduced by the co-immobilization technique; and the cross-linking of GOx, resulting in decreased inhibition in the presence of H 2 O 2 [105].
Vafiadi et al. reported similarly promising results for the use of combi-CLEAs on three commercial enzyme mixtures exhibiting feruloyl esterase activity.The authors reported on the immobilization via aggregate cross-linking and assessed its kinetic activity relative to free enzyme in ternary mixtures of n-hexane, 1-butanol, and water.Combi-CLEAs were designed to retain maximal catalytic activity by the evaluation of 10 aggregating agents, while the efficiency was optimized by varying the concentration of the cross-linking agent.A product yield of 97% was reported upon the enzyme's precipitation via ammonium sulfate and cross-linking at a glutaraldehyde concentration of 100 mM.The use of ammonium sulfate with this precipitating agent was found to be advantageous because its solvation is an endothermic reaction.Notably, the activity of enzyme aggregates prior to cross-linking were found to be higher than that of the free enzyme, supporting evidence that suggests suitable immobilization techniques can lock enzymes in highly active conformations.Furthermore, combi-CLEAs were easily separated from the reaction mixture containing unreacted methyl ferulic esters, synthesized 1-butyl ferulate by centrifugation and later reused for furuloyl esterase activity, though the immobilized enzymes showed poor activity retention and stability [102].
Martins et al. formed magnetic cross-linked enzyme aggregates (mCLEAs) from rhamnopyranosidase (Rhmnase), a hydrolytic enzyme applicable in the production of valuable pharmaceutical compounds such as lipoprotein associated phospholipase A2 inhibitors, which are administered in the treatment of atherosclerosis [110].Such magnetic aggregates were evaluated for their catalytic activity with different cross-linking and precipitating agents and subsequently compared to CLEAs@Rhmnase for reusability in a batch reactor system.CLEAs@Rhmnase were found to retain nearly 100% activity after 5 reutilization cycles of 24 h each, however, the CLEAs@Rhmnase showed a significant loss in activity after 7 reutilization cycles.Conversely, mCLEAs@Rhmnase showed an initial loss in activity of approximately 40% after one reutilization cycle and near constant activity thereafter, likely due to the higher physical stability of the magnetic aggregates.Additionally, mCLEAs@Rhmnase were shown to be more catalytically active and efficient than CLEAs@Rhmnase, suggesting that the selection of immobilization materials should be critically assessed for ensuring high biocatalytic turnover.It was determined that magnetic enzyme aggregates are more suitable biocatalysts for a scaled up process owing to improved reusability and stability [104].
Zhao et al. reported the use of CLEAs of Pseudomonas sp.lipase (CLEA-PSL) as a biocatalyst for the enantioselective resolution of (S)-N-(2-ethyl-6-methylphenyl) alanine, a chemical precursor in the production of widely used herbicides [111].Precipitation and cross-linking conditions were optimized for the formation of CLEA-PSL, and kinetic parameters were determined for free and immobilized lipase, respectively.CLEA-PSL were found to be more active than the free lipase, and it was also noted that 48 h were required for free lipase to reach a substrate conversion of 50%, while only 12 h were needed for the immobilized lipase to achieve the same conversion and enantiomeric excess.The time difference was presumably due to an induced change to a more catalytically active enzyme conformation upon immobilization.The evaluation of kinetic parameters determined that CLEA-PSL also showed an improved affinity for substrate, most likely due to the changes in the enzyme's secondary structure caused by immobilization.Lastly, the immobilized lipase was found to be more thermostable than the free lipase and retained nearly 80% of its initial activity after ten reutilization cycles in a batch reactor and with no reported loss of enantioselectivity [103].
Enzyme aggregate cross-linking has also been proposed as a method to improve biocatalysts that are currently employed on an industrial scale.Illanes et al., for instance, implemented CLEAs from recombinant penicillin acylase for the production of cephalexin with increased enzyme stability and global productivity (g cephalexin g −1 biocatalyst).Free penicillin acylase was found to require a lesser reaction time, but CLEAs were advantageous for preservation of enzyme activity.Free enzyme retained 50% residual activity after 30 h, while CLEAs retained an equal amount of activity after 78 h.Furthermore, CLEAs were found to have an increased total specific productivity (135.5 g cephalexin g −1 biocatalyst) than that of free penicillin amylase (of only 40.1 g cephalexin g −1 biocatalyst) with such an increase in the reusability, justifying a slight loss of catalytic activity due to an overall increase in the production potential of cephalexin [101].
Lastly, the intramolecular cross-linking of non-aggregated enzymes has been investigated as a method of inducing increased rigidity to and preventing non-specific protein-protein associations of multimeric enzymes, thus preserving catalytic activity when coupled with another immobilization method [112][113][114].Dinu et al. demonstrated that cross-linking of perhydrolase S54V (AcT), i.e., an enzyme that catalyzes the perhydrolysis of propylene glycol diacetate to decontaminant agent peracetic acid, allowed for the novel integration of nanobiocatalytic conjugates with latex-based paint that led to the formation of a bioactive decontaminating composite.The study also found that AcT, cross-linked with polyfunctional aldehyde dextran, retained a greater degree of catalytic activity when covalently bound to single walled carbon nanotubes (SWNTs) as compared to covalent bonding of AcT to SWNTs.The superior activity retention of AcT was attributed to cross-linking with aldehyde dextran that conferred increased rigidity to the enzyme and led to the preservation of its secondary structure upon covalent immobilization onto SWNTs [112].

Intensified Approach for Designing Improved Biocatalysts
The combination of different technical expertizes has allowed for the improved design of immobilized biocatalytic processes, but profitability seems to remain the determining factor for further enzyme-induced process development and implementation [5,115,116].For instance, significant progress was made in protein design via directed evolutionary approaches, with such progress allowing for improved activity, stability, and substrate affinity, as well as reduced costs to isolate enzymes [1,3,23,28,29].Directed evolution requires the administration of random mutations to the amino acids constituting an enzyme, which could be employed through chemical mutagenesis or DNA shuffling, followed by screening for the desired phenotype and the isolation of genes coding for any identified improved genetic variant [3].As a result of such an advance, research teams have been successful not only in developing biocatalysts that may be deployed at high temperatures and extreme pH conditions, but also biocatalysts that have catalytic activities of several orders of magnitudes greater than those of naturally occurring ones [1,3,29].For instance, researchers at Codexis and Merck successfully used such multiple iterations to increase enzyme-substrate affinity and to design an economically competitive enzyme-catalyzed process for subtilisin, a pharmaceutical used for diabetes treatment [2].However, while progress in protein engineering has helped drive increased applications in industrial enzyme catalysis, it has not addressed all limitations, like the poor mechanical stability and limited reusability of the biocatalysts, the costs associated with their in vitro production, or further adoption in commercial-scale processes [5,[20][21][22][23][24][25][26][27].
Molecular dynamics simulations (MDS) provide atomic level understanding of phenomena that determine the physical and catalytic characteristics of an immobilized biocatalyst [117][118][119][120][121]. Studies of molecular dynamics simulations, which carry out numerical integration of Newton's laws of motion at an atomistic scale, have been used to predict the structures and catalytic properties of enzymes at the molecular level, which have been extended to research in enzyme immobilization where accurate characterization of enzyme-carrier interactions provides insight into binding mechanisms [117][118][119][120].An understanding of atomistic-level interactions has led to the development of efficient and optimized biodevices.For instance, Franca et al. were able to determine through MDS that the active site of acetyl co-enzyme A carboxylase (ACC) had a positive surface potential.This insight into ACC was used to devise an optimal electrostatic adsorption of ACC onto an AFM tip for improved biodevice functionality [119].
Basso et al. performed molecular simulations on endo-and exoinulinase to explain differences in regioselectivity between the two structures.Analyses of the three-dimensional structures were subsequently used to formulate an optimal immobilized biocatalyst that showed hyperactivity when compared to its native structure [118].Qu et al. employed molecular simulations of a hydrolase MfphA adsorbed onto single walled carbon nanotubes (SWNTs) for verification of and insight into analytical results.Molecular modeling results illustrated preferred binding of two particular amino acids Trp201 and Met81 to carbon nanotubes, thus resulting in a loss of hydrolase activity due to blocking of the active site [117].Studies using molecular simulations have illustrated the utility of computational modeling in optimization of immobilization techniques to reduce lab material costs and create insight into molecular phenomena for the development of optimal immobilized biocatalysts [117][118][119][120].
These analyses show that the adoption of critical evaluation criteria for immobilized enzyme processes on multiple scales-including molecular-level modeling and analysis, life cycle assessments, and techno-economic analyses-is paramount for economical scale-up [5,44,[50][51][52][117][118][119][120].Ultimately, the appropriateness of immobilized biocatalyst for industrial processes boils down to the fruition of additional profitability with the immobilized form of an enzyme often having to hold multiple benefits over free enzyme for process economics that could overcome the additional costs and constraints associated with the immobilization to a reduced loss of catalytic activity, while balancing the cost used for covering the possible materials to be used as supports [5,116].

Glucose Isomerase: A Model for Enzyme Immobilization
The immobilization of glucose isomerase (GI) is considered an excellent model for commercial application of an immobilized enzyme where GI efficiently catalyzes the conversion of d-glucose to d-fructose in the production of high-fructose syrup (HFCS) [122].The enzymatic production of HFCS was previously determined to be more economically competitive than conventional chemical methods requiring alkaline catalysis due to improved product quality, simplified production route, and reduction of undesired byproducts such as mannose and psicose [122].
Much research has been done on immobilization of GI since its development and first industrial use in 1967.A broad range of immobilized GI products have been sold by producers like Genencor, DuPont, Novozymes SA, and Solvay, and continued progress in GI immobilization has yielded iterative improvements in the production of HFCS using immobilized GI as biocatalyst [5].In most contemporary processes, HFCS is produced in continuous fixed bed reactors containing immobilized GI as catalytic packing, which results in a mixture of nearly 42% d-fructose, 50% d-glucose, and small amounts of other sugars, and a 55% mixture of d-fructose-required for commercial application as sweetener-is attained via chromatographic enrichment [5].The success of immobilized GI is rooted in the biochemical properties of the enzyme as well as the technological developments that allowed for the commercialization of the immobilized enzyme-catalyzed process.Currently, the production of HFCS is the largest industrial process employing an immobilized biocatalyst, with a nearly 10 million tons produced per year [5].Studies showed that the temperature-dependent position of isomerization equilibrium, along with the relatively high K m value of GI, were two of the biochemical factors that drove the development of an immobilized enzyme process to improve upon a free enzyme process.At higher temperatures the equilibrium of the isomerization is shifted to favor higher yields of fructose, so the use of thermostable immobilized enzyme allowed for improved yields of HFCS.Immobilized GI allowed for implementation in continuous processes, which proved to be advantageous, as the high-concentration throughput of substrate helped to overcome the low efficiency of enzyme-substrate binding indicated by the low K m of GI.Furthermore, the production cost of GI was significant at the time of development, as the immobilization of GI decreased the total amount required for HFCS production by allowing for enzyme reuse [5].The GI-catalyzed process is being further researched for the employment of thermostable enzyme with good activity retention at 90 • C, at which point the equilibrium conditions shift such that a 55% mixture of fructose can be obtained, obviating the need for chromatographic enrichment in the process [5].

Environmental Impact Assessment and Economic Approaches for Enzyme Implementation in Industrial Catalysis
While the development of highly efficient immobilized biocatalyst is the short-term goal of lab-scale research, critical economic and environmental evaluations of immobilized enzyme-catalyzed processes are required to determine the suitability of an immobilized enzyme for scale-up [50][51][52].In such a context, life-cycle assessments (LCAs) and techno-economic analyses (TEAs) are increasingly important tools as a growing number of immobilized enzymes are assessed for commercial-scale biocatalytic processes [5].
LCAs are used to identify process energy and material requirements, as well as waste and emissions, which are subsequently used to analyze the sustainability and environmental impact of a process.The use of enzymes in industrial processes is often associated with reduced consumption of energy, chemical inputs, and waste streams.For example, using phospholipase to degum vegetable oil led to a decrease of 44 tons of equivalent CO 2 generation per 1000 tons of oil produced, due to improvement in oil yield and a subsequent decrease in feedstock requirements [5].In another study, the enzymatic production of biodiesel reduced the amount of steam needed to preheat feedstock due to milder reaction conditions, and also improved each measure of environmental impact, including human toxicity, ozone depletion, and global warming potential [50].Immobilized enzyme-catalyzed processes have been found to further reduce the environmental burden of free enzyme-catalyzed processes [51].Raman et al. performed LCA on production of biofuel from alkali catalyst, free lipase, and immobilized lipase to determine an optimally sustainable process.Both free lipase and immobilized lipase reduced process energy consumption when scaled to 1000 kg per year production due to milder reaction conditions.Furthermore, the immobilized lipase was reported to improve the free-enzyme catalyzed process because its reuse reduced consumption of carbohydrates and the minerals required for its free form production [51].The general decrease in material and energy consumption exhibited by enzymatic processes, as a result of reduction of energy consumption, indicates that biocatalytic processes are potentially both more environmentally benign and economically lucrative.However, LCA does not account for productivity or process economics, and thus is insufficient as a standalone metric for process implementation.
TEAs study the economic viability of a process based on technology readiness and process economics such as utilities, feedstocks, labor, and capital investments [52].Olafsson et al. reported a TEA for a comparison of integrated and off-site cellulase catalysis in fermentation of lignocellulosic material for ethanol production.The authors found that off-site production of ethanol using similar technologies was a more economically competitive option due to the production of more marketable byproducts [52].Analysis showed that while profitability remains the critical driving force of process development, TEAs and LCAs in combination are invaluable tools for the full diagnosis of both benefits and drawbacks associated with scale-up of biocatalytic processes.

Pharmaceuticals Industry
Enzyme catalysis has been successfully used for the production of pharmaceutically active chemicals at the industrial scale.The most significant advantages enzyme catalysis holds over conventional catalysis are the high regio-, chemo-, and stereoselectivities at which enzymes convert substrate to product [2,123].A high degree of product specificity is largely desirable in such pharmaceutical processes due to the streamlining of product synthesis routes and subsequent improvement in process economics [8,30].For example, the production of many pharmaceuticals requires the introduction and subsequent removal of protecting groups from pharmaceutically active ingredient intermediates to ensure adequate product selectivity.The use of appropriate enzymes not only obviates such steps, but has also been shown to yield higher enantiomeric excesses of desired stereoisomers [8].Furthermore, enzyme-catalyzed synthesis routes often reduced or eliminated the need for chemically harsh substances or high-temperature conditions that can otherwise require intense process safety considerations [2].
The interdisciplinary approach that allowed for the economically advantageous implementation of biocatalysis on the industrial scale is highlighted by the development of the enzyme-catalyzed synthesis of sitagliptin, a drug marketed by Merck for type II diabetes treatment [31].Sitagliptin is a dipeptidyl peptidase-4 inhibitor that prevents an increase in the blood-retinal barrier and inhibits diabetes-induced tight junction disassembly [32].Conventional synthesis of sitagliptin requires a high-pressure hydrogenation of enamine via a rhodium-based catalyst and subsequent carbon treatment to remove trace amounts of rhodium, yielding sitagliptin in 97% enantiomeric excess (e.e.) [100].Research teams at Codexis and Merck conducted extensive protein engineering to produce an R-selective transaminase (R-ATA) from Arthrobacter sp.capable of converting 200 g L −1 of prositagliptin ketone to sitagliptin in dimethyl sulfoxide (DMSO) in greater than 99.95% e.e. [33].In addition to a yield of higher enantiomeric purity, the enzyme-catalyzed route had 10% increased yield and a 53% increase in productivity (kg sitagliptin L −1 day −1 ), and also eliminated the need for a rare heavy metal-based catalyst that necessitated purification and special equipment necessary for high-pressure operation [31].
Codexis and Merck have also invested heavily in research for scale-up of a monoamine oxidase (MAO)-catalyzed process for enantiomerically pure desymmetrization of a bicyclic proline intermediate, an important precursor in the synthesis of boceprevir, a NS3 protease inhibitor used for treatment of chronic hepatitis C infections [2,34].Conventional synthesis of bicyclic proline is an intensive process requiring an excess of metal-based oxidant and reductant through 8 reaction steps; the enantioselective, MAO-catalyzed synthesis of the intermediate is an attractive alternative with the potential to greatly reduce operation time and waste generation [30].Although significant improvements in MAO activity, solubility, and thermostability were achieved through protein engineering via 4 rounds of evolution involving the introduction of random mutations and subsequent screening for desired phenotypes, the addition of bisulfate to the MAO-catalyzed process for the capture of imine compounds was necessary to mitigate its irreversible inhibition [30].
The combination of biocatalysts genetically, engineered for robust catalytic capabilities and topological process optimizations, illustrates the overlap of technical expertise needed for successful scale-up of enzyme catalysis [2,5].As such, the enzyme-catalyzed process showed several marked improvements over the conventional synthesis of the intermediate when compared at the same scale, namely decreases of 59.8% in raw materials, 32.8% in water, and 63.1% in process waste per unit of product synthesized [30].Though further work is needed for economically feasible industrial-scale implementation, the comparison between the conventional synthesis and the MAO-catalyzed synthesis route suggests that enzyme catalysis could greatly improve over current industry standards and outcomes.
In a study by Hayes et al., authors reported an improved commercial-scale synthesis route for (S,S)-reboxetine succinate, a noradrenergic anti-depressant for the treatment of fibromyalgia in the latter stages of development at Pfizer [35,36].The production of reboxetine requires an acetylation of diol intermediate.However, conventional synthesis routes rely on classical chemical acetylation that suffers from di-acetylation and poor enantioselectivity, therefore generating considerable amounts of unwanted byproducts [2].The proposed generation synthesis route successfully employed Candida antartica lipase B, an active, commercially available enzyme, for the highly enantioselective acetylation of diol intermediate [36].The lipase-catalyzed process resulted in the selective mono-acetylation of diol intermediate with 98% regioselectivity and greater than 99% yield.Furthermore, it was possible for the enzyme to be removed from the reaction mixture via simple filtration, and it thus maintained high regioselectivity at the lab scale upon reuse, all at low costs [36].As such, the new generation synthesis route resulted in a 58% improvement in the commercial product yield of (S,S)-reboxetine succinate and a nearly 1300 MT year −1 reduction in process waste at peak process throughput [36].
Because lipases can hydrolyze a broad spectrum of substrates, they have been researched as biocatalysts for many other pharmaceutical syntheses [7,36,37].Martinez et al., for instance, proposed a new-generation synthesis route for the industrial-scale production of pregabalin, a neuroactive drug exhibiting anticonvulsant, pain killing, and anti-anxiety activity used for the treatment of epilepsy, anxiety, and social phobia [37,38].The proposed route utilized lipolase, a commercially available lipase, for the selective hydrolysis and subsequent separation of the S-enantiomer intermediate from the R-enantiomer for conversion to pregabalin via decarboxylation [37].While screening for industrial enzyme increased selectivity and reduced waste generation, several process optimizations allowed for the improved process economics of such a new-generation route.The addition of Ca 2+ and Zn 2+ mitigated lipolase inactivation by forming complexes with chemical species that acted as enzyme inhibitors.In addition, the rapid phase splitting of S-enantiomer from R-enantiomer enabled racemization and thus efficient reuse of starting material [37].The new-generation synthesis route resulted in a 40-45% increase in yield of pregabalin at 99.5% purity and 99.75% e.e.Furthermore, the amount of waste generated per kilogram of product yielded was calculated to be just 20% that of the classical route [37].

The Food-Water-Fuel Nexus
The large-scale production of biofuels, i.e., fuels derived from biomass, animal fats, waste oils, and other renewable resources that encompass chemical products such as bioalcohols, biodiesel, biosynthetic oils, and biogas, has been recognized for its potential to supplement or replace fossil fuels, particularly as oil reserves are depleted to meet global energy demands [18,49].The benefits of economically feasible biofuel production are two-fold: biofuel offers improved sustainability over traditional fuel sources as well as significantly reduces the environmental impact owing to its lower emission of carbon monoxide, nitrogen oxides, sulfur oxides, and particulate matter [18].According to British Petroleum (BP), global production of biofuels rose by an average of 14.1% from 2006 to 2016, illustrating the growing impact that biofuels have on the world energy landscape [124].While government incentives have helped to drive industry-scale biofuel production, the economic viability of biofuel production will be determined by the development of processes that efficiently use waste from agriculture and industry as feedstock, thus side-stepping the ethical dilemma of using fresh water and land resources for fuel production [125].Numerous technologies exist for the conversion of raw biological materials to usable, high-energy bioproducts with most production routes requiring either the transesterification of oils or the esterification of fatty acids [5,18].The traditional chemical process uses sodium methoxide for conversion of plant oil triglycerides to fatty acid methyl esters (FAMEs), which subsequently results in a product contaminated with high alkali salt content requiring costly purification [5].
The use of lipase as a biocatalyst for esterification was researched for its efficiency at mild reaction conditions and high-purity product yields and was proposed to eliminate the need for purification.However, for biofuel production, economical implementation of such an enzymatic process requires efficient recovery and reuse of lipase due to the required scale of production, therefore necessitating an immobilized enzyme [5,49].
A prominent trend in the production of biofuels is the design of processes based on inexpensive, largely abundant starting materials because cheap feedstock is one of the biggest driving forces in the profitability of biofuels processes [19].The biggest such feedstock is lignocellulosic biomass-made up of lignin, cellulose, and hemicellulose-due to its massive abundance and wide range of sources including crop residues, softwood and hardwood, herbaceous biomass, and municipal solid waste [19].The most difficult technical barrier required to unlock lignocellulosic biomass is however the extensive mechanical or chemical pretreatment required to further enable the processing of cellulose in lignocellulosic biomass via hydrolysis to glucose and subsequent conversion to bioethanol via whole-cell fermentation [126][127][128].Current research suggests that ionic liquids, i.e., salts that exist in molten states at temperatures below 100 • C with strong chemical and thermal stabilities and extremely low vapor pressures, will play a key role in the development of processes that viably release cellulose from lignocellulosic material [126][127][128].
Chemical conversion of cellulose to glucose requires the use of diluted acids and high temperatures, which implies high energy inputs to result in the generation of a significant amount of unwanted byproducts [19].A more ideal approach is the use of cellulase, i.e., a mixture of hydrolytic enzymes that act synergistically in the conversion of cellulosic material, for the selective enzymatic hydrolysis of cellulose to glucose, which requires longer reaction time but leads to improved yield from the subsequent fermentation due to a low generation of unwanted byproduct [19,[53][54][55].The economically viable scale-up of cellulase-catalyzed cellulose conversion to glucose for bioethanol production is limited by poor biocatalyst recovery, slow enzyme-catalyzed reaction rates, and low biocatalyst stability under industrial operation conditions [54,55].Therefore, immobilized cellulase is a requirement for industrial catalysis, particularly considering the acid pretreatment required for cellulose [55].
Reported lab-scale work on the immobilization of cellulase illustrates the potential for the employment of cellulase for biodiesel production.Khorshidi et al., for instance, showed that immobilized cellulase was significantly more active than free cellulase at lower pH conditions and at higher temperatures, showing that the immobilization technique can functionalize biocatalyst at industrial conditions [54].Lima et al. found that immobilized cellulase had increased thermostability when compared to free cellulase and retained nearly 70% of its initial activity after eight cycles of converting cellulosic biomass to glucose.The significant activity retention of immobilized cellulose suggests that immobilization techniques can improve process economics by allowing for efficient reuse of biocatalyst [56].
In 2006, Hainabaichuan Co. Ltd. (Guangzhou, China) began processing waste palm oils and waste edible oils for lipase-catalyzed production of 20,000 tons of biodiesel per year and subsequent scale-up to 40,000 tons per year in 2008 [49].Lvming Environmental Technology Co. Ltd.(Shanghai, China) implemented a commercially available immobilized lipase as a catalyst in a FAME production line with an annual capacity of 10,000 tons in 2007 [57].The enzymatic reaction, designed for a feedstock of high acid value (AVN160 mg KOH g −1 ) waste cooking oil, was carried out in a stirred tank reactor at an enzyme loading of 0.4% relative to charged substrate, which led to a FAME yield of 90% at optimal conditions [57].Piedmont Biofuels announced in 2012 the successful scale-up of a continuous enzymatic transesterification of free fatty acids (FFAs) via immobilized Candida antarctica lipase B for biodiesel production; the enzymatic reaction eliminated the need for caustic stripping of the chemical intermediate due to its high product selectivity [5].

Natural Gas Conversion
Recent advances in the extraction and recovery of natural gas resources have made accessible vast reserves of natural gas in North America [129].A review of world energy production and energy markets by BP reported a proven reserve of nearly 8.7 trillion m 3 of natural gas in the US alone [124].Additionally, production of natural gas in the US comprises over 20% of global natural gas production at 750 billion m 3 in 2016 [124].
The composition of natural gas is 80-95% methane with varying degrees of heavier hydrocarbons, but methane has a relatively low market value due to difficulties in storage and transportation as well as limited use as fuel [129,130].The low market value and high greenhouse gas potential of methane have initiated a surge in research and development of technologies that can be employed to convert methane to high-quality, value-added chemicals.
Much current research on the economically viable use of methane as feedstock is focused on conversion to methanol, which can be more readily converted to olefins and other valuable hydrocarbons [130,131].Current processes for a traditional chemical conversion of methane to methanol, such as steam reformation and the Fischer-Tropsch process, are limited by several significant drawbacks.The chemical conversion route requires the use of high-temperature, high-pressure unit operations as well as noble metal catalysts, resulting in a poor selectivity of methanol [130].The low yield of methanol necessitates a large process throughput in order to overcome large capital cost investments, thus the process is only profitable at a massive scale, thus placing further constrictions on process employment due to the difficulty of transporting methane from an extraction site to a production plant [129,130].
The use of the biocatalyst methane monooxygenase (MMO), for the conversion of methane to methanol, has recently gained interest in the wave of expanding natural gas extraction.MMO has been shown to convert methane to methanol at ambient conditions with selectivity approaching 100% and thus has been researched for the scale-up of methane conversion, considering its multiple advantages over the chemical conversion route [14,[129][130][131].The high selectivity of MMO-catalyzed methanol production eases the intensity of product separation and could significantly reduce the number of steps required in the conversion process.Furthermore, the enzymatic reaction occurs at mild reaction conditions, and could thus cut back on costs associated with heating, pressurization, and other feedstock conditioning steps [129,130].
Much lab-scale research remains to assess the MMO-catalyzed conversion of methane for industrial applications because isolation of MMO is an intensive process, suggesting it may be beneficial to immobilize the enzyme for reuse.Blanchette et al. reported the use of a 3D printed microbioreactor with immobilized MMO as packing for continuous methane conversion to methanol.Although immobilized MMO retained good activity through 20 consecutive reuses, the overall product yield was significantly less than the biocatalytic mass required for methane conversion [129].Ultimately, enzymatic conversion of methane to methanol is a developing technology with several major hurdles to overcome before successful economic scale-up.Due to the inexpensive costs of methane and methanol, enzyme-catalyzed process must be efficient to ensure economic viability.Currently, low catalytic activity of MMO is a significant limiting factor [14,15,[129][130][131]. Furthermore, a more intensive examination of process configurations is required to mitigate the mass transfer limitations that arise from the low solubility of oxygen and methane in aqueous media [130].

Food and Beverage Industry
In many instances, traditional chemical synthesis routes are not viable for food products due to reagent toxicity and complex reaction chemistries that result in unfavorable process economics [11].Biocatalysts, on the other hand, present an opportunity for simplified, efficient production routes that mitigate the need for harsh substances, and thus are more economically competitive [9][10][11]132].As such, the use of biocatalysts in food and beverage processes dates back thousands of years to the advents of culinary practices like wine and cheese making [10].In modern times, the widespread use of enzymes in food and beverage industries for food quality preservation or modification is one of the earliest successful industrial applications of biocatalysis, observed in beer fermentation, juice debittering, and bread baking [133].The replacement of conventional chemical treatment with enzyme-catalyzed pathways for conversion of starch to glucose and fructose first took place several decades ago [12,122].
The conventional production route requires temperatures up to 175 • C and considerable pressurization, whereas biocatalytic processes can be carried out at temperatures near 100 • C and at ambient pressure via sequential α-amylase-catalyzed reactions encompassing both liquefaction and saccharification steps [122].In addition to milder reaction conditions, the multi-enzymatic process resulted in higher product selectivity and therefore allowed for better defined production routes for varying sugar products like maltose, fructose syrup, and crystalline sugar, as dictated by biocatalyst selection [122].
An emerging trend is the use of enzyme catalysis for commercial-scale production of probiotics, artificial sweeteners, and rare sugars [2].Probiotics, such as oligosaccharides, lactulose, lactilol hydrolysates, and inulin, are non-digestible food additives that stimulate growth of gut bacteria and can reportedly improve human health [2,9,133].Dietary supplement producers have become particularly interested in simple, efficient enzyme-catalyzed synthesis routes of probiotics due to above-average projected market growth and accompanying increase in demand [2,9].Yakult Honsha Co. Ltd. of Japan and Friesland Food Domo of The Netherlands, among others, have carried out commercial-scale, enzyme-catalyzed production of galacto-oligosaccharides (GOS), a lucrative probiotic with digestive health benefits and use as low-calorie sweeteners [9].GOS are produced by transgalactosylation simultaneous to hydrolysis of lactose via β-galactosidase; lab-scale results have shown GOS yields near 40% for free enzyme, while immobilized enzymes show the potential for larger yields of up to 50% through implementation in a continuous system resulting in decreased product inhibition [9].
The enzymatic production of protein hydrosylates for use as nutritional supplements and flavor enhancers has been developed due to milder reaction conditions and increased control over product formation relative to traditional chemical routes [133].When hydrolyzed, a parent protein forms biofunctional peptides exhibiting antioxidant, antimicrobial, and antihypertensive properties, among other therapeutic effects [132,133].The production of fish protein hydrolysates from seafood processing waste via papain, a proteolytic enzyme derived from papaya that has found widespread industrial application, has garnered attention recently because the process is a potential solution for minimizing pollution from fishing industries [39].Additionally, papain has been researched as a biocatalyst for production of protein hydrosylates from Chinese walnuts; lab-scale work on papain catalysis has shown moderate yields and purities of hydrosylates, and peptides obtained from produced hydrosylates showed good antioxidant properties [40].

Flavors and Aromas Industry
Biocatalytic processes economically and environmentally advantageous to conventional chemical processes are in development for commercial-scale production of fragrance compounds, flavor compounds, and aromatics.The chemical structures of such substances are often characterized by regio-, chemo-, or stereoselective positioning of functional groups like alcohols, aldehydes, ketones, and esters; thus, scale-up of efficient enzyme-catalyzed processes for production of aromatic compounds is a potentially lucrative endeavor, particularly in light of promising global market projections for flavors and fragrances [42].
Lipase enzymes play an integral role in the development of biocatalytic fragrance and flavor production due to its capability to transfer acyl groups from esters to other nucleophiles [42].Though most progress has been in bench-scale synthesis of aroma esters, the results suggest that a combination of protein engineering and process engineering can facilitate scale-up to profitable industrial processes.For instance, Vosmann et al. achieved a 94% conversion of oleic acid selectively to benzyl oleate with benzyl alcohol as an acyl acceptor in 1 h using a commercially available lipase, which illustrates strong product specificity that could make enzymatic conversion advantageous [41].Badgujar et al. reached 99% conversion of vinyl propionate to p-cresyl propionate in 1.5 h via immobilized lipase in heptane, highlighting that certain immobilization techniques could functionalize lipase for implementation in industrial environments [43].However, further work to achieve more efficient, robust biocatalytic reactions is required before enzymatic production of aromatic esters can be scaled to larger process throughputs [41][42][43].

Detergents Industry
Successful employment of biocatalysts is cited as the driving force of production of cost effective, environmentally benign detergents [44].In the instance of the detergents industry it should be noted that enzymes are a product rather than a chemical process-specific catalyst.Nonetheless, favorable market trends in the detergents' industry reinforce the underlying view that biocatalytic products are inherently safer and more sustainable than traditional chemical products that pose health and safety risks [44][45][46].Alkaline proteases-which are effective in the removal of protein stains and the cleaving of damaged cotton fibers-isolated from microbial sources comprise significant portions of multiple detergents produced and sold at commercial scale by manufacturers like Novozymes SA, Kao Corporation, and Genecor International [45].The high reaction specificity of enzymatic reactions further mitigates damage to fabrics and surfaces that is characteristic of chemically harsh detergent agents [47].Furthermore, the ratio of catalytically active enzymes in detergent mixtures are optimized for specific detergent applications; for instance, dishwashing detergents often contain varying degrees of amylase and lipase intended for the removal of starch food deposits and fats and oils, respectively [47,48].

Projections of Economic Growth and Implementation Potential
The market for industrially relevant enzymes-including those applied in food, animal feed, detergent, and technical industries-is expected to grow globally through 2021 at an annual growth rate of 4.7%.Development of novel enzyme technologies, increased demand for naturally made food products, and policy requiring larger shares of renewable energy sources like biofuels [44] are not only becoming an alternative but an implementation reality.Of these categories, enzymes used in technical industries such as papers, textiles, leathers, and biofuels comprise the greatest potential for use of immobilized biocatalysts owing to process-oriented application [44].Thus, market projections for technical enzymes are a reasonable indicator of process development for biocatalytic processes designed for commercial-scale production lines.
The most significant driving force in projected market growth for technical enzymes is renewed interest in enzyme-catalyzed biofuel production using lignocellulosic materials, which is a direct result of stricter environmental regulations [44].In this regard, enzymes used in biofuels processes represent the largest portion of the technical enzyme market, which is dominated by two major players, Novozymes and Danisco/DuPont [44].In 2015, DuPont and Quad County Corn Processors signed a multiyear contract for the production of bioethanol from cellulosic material in corn kernel fiber; the same year, DuPont acquired enzyme production technology assets from Dyadic, which is expected to further drive process development in the technical enzyme sector [44].The same technologies are being investigated for further integration into paper and pulp industries, where xylanase, catalase, and lipase are frequently used for biomechanical pulping, de-inking of recycled fibers, and modification of fiber properties [58][59][60][61].
The bulk of the global technical enzyme market is reported for markets in Europe, the Middle East, and Africa (EMEA) at nearly 35% of the global share in 2016 [44].However, strong market growth is projected for North America and Asia-Pacific, with annual growth rates of 6.8% for North America and 7.9% for the Asia-Pacific region through 2021 (Figure 3) [44].Furthermore, the technical enzyme market is expected to overtake the EMEA market as the largest global segment of the market by 2021, suggesting progress in process development will largely take place in the Asia-Pacific region and in North America through 2021 [44].
If further progress is to be achieved, optimizing the structure-function relationships of enzyme-based conjugates while minimizing the production costs of the individual components (i.e., support and biocatalysts), as well as the implementation costs of the biocatalytic process is imperious (Figure 4).The modification of conventional schemes for enzyme immobilization and integration will not only need to facilitate product yield but, furthermore, optimize the process' metrics to thus limit logistical burden to both the environment and the individual [134][135][136].Coupling or transient enzyme-driven reactions will also aim to reduce any public pressure associated with implementation of fossil fuels and thus lead a safer and eco-friendly solution for industrial revolution.
The challenges and opportunities associated with enzyme catalysis implementation will not only have to consider profit and marketability, but it will further have to consider integration of biomimetic approaches or "one pot" processes that could allow for efficiency, profitability, reusability and stability, all under the complexity of multi-chain driven specific reactions.  .Schematic representation of a multidisciplinary approach aimed to define optimal biocatalytic processes.Implementation of biocatalysts in industrial technologies will have to not only consider optimization of the enzyme functionality but further, lead to increase in enzyme operational stability at the interface with supports used for immobilization.

Figure 1 .
Figure 1.Global enzyme market in 2016 (top) and projected global enzyme market in 2021 (bottom).Figure adapted from data included in Ref. [44].
Figure 1.Global enzyme market in 2016 (top) and projected global enzyme market in 2021 (bottom).Figure adapted from data included in Ref. [44].

Figure 3 .
Figure 3. Global market growth projections for technical enzymes by geographical regions.

Figure 4
Figure 4. Schematic representation of a multidisciplinary approach aimed to define optimal biocatalytic processes.Implementation of biocatalysts in industrial technologies will have to not only consider optimization of the enzyme functionality but further, lead to increase in enzyme operational stability at the interface with supports used for immobilization.

Table 1 .
Industrial applications of enzyme catalysis.