Biocatalysis and Biotransformations

This editorial work was possible because the funding received from the European Union’s 
Horizon 2020 research and innovation program (Blue Growth: Unlocking the potential of Seas and Oceans) 
through the Project ‘INMARE’ under grant agreement No. 634486, the grants PCIN-2014-107 (within ERA 
NET IB2 grant nr. ERA-IB-14-030—MetaCat), PCIN-2017-078 (within the ERA-MarineBiotech grant ProBone), 
BIO2014-54494-R and BIO2017-85522-R from the Spanish Ministry of Economy and Competitiveness, and the 
European Regional Development Fund (ERDF).

With the discovery of the mechanisms by which proteins fold and the development of protein engineering and computational tools, the engineering and re-design of natural enzymes or even the design of enzymes from scratch became possible [9][10][11]. Whatever the capacity to identify or engineer new and better performing enzymes, it is increasingly recognized that biocatalysts are delicate materials that need to be stabilized to survive a range of challenging conditions typically used in industrial processes and any conversion in general. Thus, in order to construct efficient enzymes, they need to be stabilized. Distinct combinatorial and rational methods to generate such stabilized biocatalysts are required, regardless of the apparent relative improvements at the end of the process [12].
Whatever the utilization of free or immobilized enzymes (in the form of native or engineered proteins or whole cells), the production of chemicals by enzyme catalysts offers a promising opportunity to improve overall economics and sustainability. Indeed, the overall bio-based products currently displace about 300 million gallons of petroleum per year, which is equivalent to taking 200,000 cars off the road [1]. Also, a reduction in greenhouse gas emissions for bio-derived products relative to their petroleum counterparts was demonstrated [1]. The market for bio-based chemicals reached more than $20 billion in 2018 and is expected to continue growing. Identifying enzymatic catalysts capable of transforming an ample set of chemicals, including those present in different feed-stocks, may be of interest to broaden enzyme applicability and process developments. This will contribute to reach the ambitious objectives of biotechnology programs such as the Bio-based Industry (BBI) program (http://biconsortium.eu), whose objective is to promote the production of new bio-based products.

The Present Issue
I am honored to be the Guest Editor of a thematic issue published in the Catalysts journal for the first time. I would especially like to thank Jiuyu Guo, Senior Assistant Editor, and the entire staff of the Catalysts Editorial Office, who productively collaborated with me. Furthermore, I would like to thank all of the authors contributing their papers to this issue for their excellent work. It is comprised of twenty-five research articles. As pointed out in the background, the meaning of this issue is to underline new trends in Biocatalysis and Biotransformations. With this aim, special attention has been paid to identifying, engineering, and immobilizing known or new enzymes so as to increase the progress and diversity of enzyme conversions, as well as describing recent advances in the applications of enzymes (free or immobilized) and whole cell transformations for the synthesis of valuable chemicals.
Among the research articles presented in this issue, an ample set of enzymes have been investigated for biocatalytic processes. They include hydrolases (esterases, lipases, hydantoinase, and beta-galactosidases), transferases (2 -deoxyribosyltransferases, hypoxanthine-guanine-xanthine phosphoribosyltransferases, and methionine adenosyltransferases), and oxidoreductases (CYP153A monooxygenase, carboxylic acid reductase, aldehyde reductases, 3β-hydroxysteroid dehydrogenases, Baeyer-Villiger monooxygenases, polycyclic ketone monooxygenases, nicotine hydroxylase, pyrroloquinoline quinone-dependent alcohol dehydrogenase, glutathione peroxidase, laccase, amine dehydrogenases, and NADH oxidases), as well as racemases and 3β-hydroxysteroid isomerases. Most studies reported the use of free or immobilized enzyme preparations, but whole cells transformations using cells expressing desired enzymes or new strains containing enzyme activities of interest have been also presented. Note that oxidoreductases, transferases, and hydrolases are among the three types of enzymes most used at an industrial level [1,4].
The biotransformations covered in this issue include those involved in the synthesis of many value-added chemicals, such as nucleoside, purine, pyridine, steroid, α,ω-diols (C 8 -C 16 ) and furan derivatives, 2-ethylhexyl salicylate (which is an organic compound used as an ingredient in sunscreens and cosmetics to absorb UVB (ultraviolet) rays from the sun), optically active sulfoxides and amines, L-amino acids, molecules with probiotic properties (such as lactulose esters and oligosaccharides), and other molecules with a wide application in the field of medicine (such as S-adenosylmethionine). In all cases, the enzymatic syntheses were shown to represent an attractive alternative to chemical synthesis because of their high specificity, high efficiency, and low production of pollutants.
In more detail, Fresco-Taboada and coworkers investigated the synthesis of modified nucleosides by using novel 2 -deoxyribosyltransferases [13]. Acosta and coworkers reported the synthesis of purine derivatives by the sequential action of 2 -deoxyribosyltransferases and hypoxanthine-guanine-xanthine phosphoribosyltransferases [14]. The utilization of renewable free fatty acids as a source of α,ω-diols (C 8 -C 16 ) by the action of CYP153A monooxygenase, carboxylic acid reductase, and aldehyde reductases was demonstrated by Ahsan and coworkers [15]. Huang and coworkers investigated the synthesis of 2-ethylhexyl salicylate using lipases [16]. García-Fernández and coworkers investigated the synthesis of 4-ene-3-ketosteroids by using 3β-hydroxysteroid dehydrogenases/isomerases [17]. De Gonzalo and coworkers successfully reported the synthesis of optically active sulfoxides using Baeyer-Villiger monooxygenases and polycyclic ketone monooxygenases [18]. The conversion of 6-hydroxy-3-succinoylpyridine into 2,5-dihydroxypyridine by nicotine hydroxylases was investigated by Wei and coworkers [19]. The synthesis of S-adenosylmethionine was investigated by Niu and coworkers using methionine adenosyltransferases [20]. L-amino acid production was investigated by Rodríguez-Alonso and coworkers who used double enzymatic systems based on the application of racemases and hydantoinases to produce norvaline, norleucine, and homophenylalanine [21]. The conversion of furans, including 5-(hydroxymethyl) furfural (HMF) and furfural, by Bayer-Villiger monooxygenases was investigated by Kumar and Fraaije [22]. Chávez-Flores investigated the application of lipases for the synthesis of lactulose esters [23]. Whole cells biocatalysts containing amine dehydrogenases and NADH oxidases were also employed for kinetic resolutions of racemic amines by Jeon and coworkers [24]. Whole cells of new isolates were also employed by Yañez-Ñeco and coworkers, and they reported the production of galactooligosaccharides [25].
Besides the above conversions, those of complex molecules such as sugar maple bark, cellulose-based materials, and wastewater were also covered in this issue, as well as the decolorization of dyes and energy generation. In particular, the conversion of complex molecules such as sugar maple bark and cellulose-based materials into several chemical backbones was investigated by Koumba-Yoya and coworkers [26] and Haske-Cornelius and coworkers [27], exemplifying the capacity of bioocatalysis as source of chemicals from complex biomasses and feedstocks. Conversion of other complex molecule-containing systems, such as wastewater, was investigated by Li and coworkers using new Mn-containing catalases [28]. Site-directed mutagenesis as a tool to improve catalytic performance was investigated by Wang and coworkers; authors introduced mutations into a bacterial laccase to improve indigo decolorization [29].
Several investigations in this issue focused on investigating the effect of different immobilization methods on biocatalyst performance. Thus, Tu and coworkers focused on improving the catalytic performance of immobilized lipase on clay/chitosan composites [30]. Sakurada and coworkers increased the long-term stability and catalytic current density of pyrroloquinoline quinone-dependent alcohol dehydrogenase when immobilized on a redox polymer, aminoferrocene, which was attached on an electrode [31]. Finally, several aminated-agarose beads were employed to increase the catalytic efficiency of beta-galactosidases via glutaraldehyde chemistry using different strategies by Zaak and coworkers [32].
Another important aspect which, in my opinion, additionally deserved research, concerns the application of methods other than protein engineering and immobilization, which could lead to more efficient biocatalysis. Two important examples are herein reported by Wang and coworkers, who designed and synthesized cyclodextrin derivatives which could serve as stable glutathione peroxidase mimics for the removal of H 2 O 2 and other hydroperoxides [33]. Similarly, Huang and coworkers design selenium-containing trypsin mimics that increase the substrate binding capacity and stability of glutathione peroxidases [34]. Maslova and coworkers investigated how lactonase activity of organophosphorus hydrolases can be increased by producing enzyme-polyelectrolyte complexes with poly-L-glutamic acid or poly-L-aspartic acid and adding small concentrations of antibiotics [35].
Finally, in the present issue, Coscolín and coworkers [36] and Nguyen and coworkers [37] investigated the properties and catalytic performance of biocatalysts newly isolated from genomes and metagenomes. In particular, Coscolín and coworkers investigated an ample set of metagenomicsderived esterases and lipases to reveal the extent by which promiscuity and selectivity co-exist in these catalytically-important biocatalysts, so as to identify chiral selective and promiscuous esterases and lipases. Nguyen and coworkers presented the complete genome sequence of Methylomonas sp. DH-1 to investigate the application of this strain for the production of succinate from methane. This is because of the presence in its genome of a set of required enzymes.
In conclusion, the present issue, "Biocatalysis and Biotransformations", is of great interest and relevance as it covers all aspects, from enzyme and microbe discovery and the investigation of their enzymatic properties, to enzyme immobilization and engineering, and to applications of important chemical scaffolds and applications related to health and energy.