Well-Shaped Sulfonic Organosilica Nanotubes with High Activity for Hydrolysis of Cellobiose

Sulfonic organosilica nanotubes with different acidity densities could be synthesized through the co-condensation of ethenylor phenylene-bridged organosilane and 3-mercaptopropyltrimethoxysilane followed by sulfhydryl (–SH) oxidation. Transmission electron microscopy (TEM) analysis and nitrogen adsorption-desorption experiment clearly exhibit the hollow nanotube structures with the diameters of about 5 nm. The compositions of the nanotube frameworks are confirmed by solid state 13C nuclear magnetic resonance (NMR) while X-ray photoelectron spectroscopy (XPS) shows that about 60–80% of SH groups were oxidized to sulfonic acid (SO3H). The acid contents were measured by both elemental analysis (CHNS mode) and acid-base titration experiment, which revealed that the acid density was in the range of 0.74 to 4.37 μmol·m−2 on the solid. These nanotube-based acid catalysts exhibited excellent performances in the hydrolysis of cellobiose with the highest conversion of 92% and glucose selectivity of 96%. In addition, the catalysts could maintain high activity (65% conversion with 92% selectivity) even after six recycles.


Introduction
The preparation of fuels and chemical products derived from biomass is one of the most attractive approaches of the sustainable development for our society [1].Cellulose, most widely existing in nature as one of the renewable biomass resources, has drawn much attention regarding its efficient utilization [2].Among the strategies for cellulose utilization, hydrolysis reaction is one of the most important steps.Due to the strong intermolecular and intramolecular hydrogen bonds, traditional acid catalysts including concentrate and diluted sulfuric acids are usually used to realize the cleavage of cellulose [3].However, such liquid acids are difficult to recycle and reuse, which may cause serious environmental pollution and high production costs [4].Therefore, considerable efforts have been dedicated to the design and synthesis of effective solid acid catalysts as alternatives.
Organic-inorganic hybrid materials are considered as a kind of novel promising catalyst for the transformation of cellulose, owing to their unique characters such as simple synthetic process, highly stable structures and tunable hydrophilic/hydrophobic properties [5][6][7].More recently, periodic mesoporous organosilicas (PMOs) incorporated with different organic moieties have emerged as useful solid catalysts in acid-catalyzed hydrolysis reactions.For example, Van Der Voort et al. synthesized sulfonic acid-functionalized PMOs through a "one-step" method, which can effectively catalyze the esterification of acetic acid and benzyl alcohol [8].Guo  2-(4-chlorosulfonylphenyl)ethyl trimethoxysilane into PMO frameworks formed by two bifunctional organosilanes and the catalysts were evaluated by the esterification of palmitic acid and transesterification of yellow horn seed oil [9].Such results clearly indicate the big potentials of PMOs incorporated with acid sites in the hydrolysis reactions.PMOs with nanotube structures have been prepared from bridged organosilane precursors through a simple micelle-templating approach [10].These nanotubes have distinct advantages such as more active sites, easy access to active sites in the tubes, and confinement effects inside the cavity.Up to now, several kinds of organic groups have been incorporated into the nanotube frameworks, such as ethenyl, phenylene and bipyridine [9,11,12].Unique properties of these organosilica nanotubes make them be widely used in heterogeneous catalysis, which could reduce the diffusion limitation and facilitate the transport of reactants and products due to the uniform nanotube structures and the large pore diameters [13,14].
Herein, through the co-condensation of organosilica precursors and 3-mercaptopropyltrimethoxysilane under acid conditions in the presence of P123 [(EO) 20 (PO) 70 (EO) 20 ] as a template agent, we have constructed sulfonic organosilica nanotubes bridged with ethenyl or phenylene in the frameworks, respectively, followed by the sulfhydryl (-SH) oxidation using hydrogen peroxide.These solid nanotube-based acid catalysts showed excellent performances in the hydrolysis of cellobiose (Scheme 1).In addition, in order to investigate the effect of surface acid density, solid catalysts composed of different amount of acidic sites were synthesized.The hydrolysis of cellobiose indicates that improved activity could be achieved with the increasing of acid density.
Catalysts 2017, 7, 127 2 of 11 synthesized sulfonic acid-functionalized PMOs through a "one-step" method, which can effectively catalyze the esterification of acetic acid and benzyl alcohol [8].Guo and coworkers inserted 2-(4chlorosulfonylphenyl)ethyl trimethoxysilane into PMO frameworks formed by two bifunctional organosilanes and the catalysts were evaluated by the esterification of palmitic acid and transesterification of yellow horn seed oil [9].Such results clearly indicate the big potentials of PMOs incorporated with acid sites in the hydrolysis reactions.PMOs with nanotube structures have been prepared from bridged organosilane precursors through a simple micelle-templating approach [10].These nanotubes have distinct advantages such as more active sites, easy access to active sites in the tubes, and confinement effects inside the cavity.Up to now, several kinds of organic groups have been incorporated into the nanotube frameworks, such as ethenyl, phenylene and bipyridine [9,11,12].Unique properties of these organosilica nanotubes make them be widely used in heterogeneous catalysis, which could reduce the diffusion limitation and facilitate the transport of reactants and products due to the uniform nanotube structures and the large pore diameters [13,14].
Herein, through the co-condensation of organosilica precursors and 3mercaptopropyltrimethoxysilane under acid conditions in the presence of P123 [(EO)20(PO)70(EO)20] as a template agent, we have constructed sulfonic organosilica nanotubes bridged with ethenyl or phenylene in the frameworks, respectively, followed by the sulfhydryl (-SH) oxidation using hydrogen peroxide.These solid nanotube-based acid catalysts showed excellent performances in the hydrolysis of cellobiose (Scheme 1).In addition, in order to investigate the effect of surface acid density, solid catalysts composed of different amount of acidic sites were synthesized.The hydrolysis of cellobiose indicates that improved activity could be achieved with the increasing of acid density.Scheme 1. Illustration of sulfonic organosilica nanotubes and hydrolysis of cellobiose.

Characterization of Sulfonic Organosilica Nanotubes
Sulfhydryl-functionalized organosilica nanotubes with ethenyl or phenylene groups in the frameworks were synthesized through the co-condensation of ethenyl-or phenylene-bridged organosilane and 3-mercaptopropyltrimethoxysilane (MPTMS) using self-assembly methods, which were denoted as SHX-Et-SNT or SHX-Ph-SNT (x means the ratio of MPTMS amounts in the total precursors), respectively.After the treatment with hydrogen peroxide, sulfonic organosilica nanotubes (SO3HX-Et-SNT or SO3HX-Ph-SNT) could be obtained.Figure 1 shows the TEM images of SH0.1-Et-SNT and SO3H0.1-Et-SNT,confirming that functionalized nanotubes before and after SH oxidation possess clear nanotube structure with several hundred nanometers in length.However, SO3H0.1-Et-SNT exhibits some aggregation, which might be ascribed to the interactions among different SO3H groups.In addition, the inner diameter from TEM images was about 5 nm and it was large enough for the free diffusion of molecules in the channels.TEM images of other mercaptopropyl organosilica nanotubes (SH0.2-Et-SNT,SH0.3-Et-SNT, SH0.1-Ph-SNT, SH0.2-Ph-SNT, and SH0.3-Ph-SNT) were shown in Figure S1.Except for some breakage of tubes observed when x = 0.3, all composite materials maintained general tubular structures.In contrast, further increasing MPTMS to x = 0.5 Scheme 1. Illustration of sulfonic organosilica nanotubes and hydrolysis of cellobiose.

Characterization of Sulfonic Organosilica Nanotubes
Sulfhydryl-functionalized organosilica nanotubes with ethenyl or phenylene groups in the frameworks were synthesized through the co-condensation of ethenyl-or phenylene-bridged organosilane and 3-mercaptopropyltrimethoxysilane (MPTMS) using self-assembly methods, which were denoted as SH x -Et-SNT or SH x -Ph-SNT (x means the ratio of MPTMS amounts in the total precursors), respectively.After the treatment with hydrogen peroxide, sulfonic organosilica nanotubes (SO 3 H x -Et-SNT or SO 3 H x -Ph-SNT) could be obtained.Figure 1 shows the TEM images of SH 0.1 -Et-SNT and SO 3 H 0.1 -Et-SNT, confirming that functionalized nanotubes before and after SH oxidation possess clear nanotube structure with several hundred nanometers in length.However, SO 3 H 0.1 -Et-SNT exhibits some aggregation, which might be ascribed to the interactions among different SO 3 H groups.In addition, the inner diameter from TEM images was about 5 nm and it was large enough for the free diffusion of molecules in the channels.TEM images of other mercaptopropyl organosilica nanotubes (SH 0.2 -Et-SNT, SH 0.3 -Et-SNT, SH 0.1 -Ph-SNT, SH 0.2 -Ph-SNT, and SH 0.3 -Ph-SNT) were shown in Figure S1.Except for some breakage of tubes observed when x = 0.3, all composite materials maintained general tubular structures.In contrast, further increasing MPTMS to x = 0.5 would lead to the collapse of nanotubes, which reveals the larger amount of MPTMS in the precursors could not form nanotubes (Figure S2a).This may be due to the unmatchable hydrolysis and condensation rate between MPTMS with a large amount and phenylene-bridged organosilane, which therefore destroying the self-assembly process.As a comparison, sulfonic pure silica nanotubes without ethenyl or phenylene in the frameworks were also synthesized, which exhibit tube structures in spite of some agglomeration shown in Figure S2b.This also indicated that organic groups in the frameworks could help nanotubes disperse from each other to obtain uniform nanotube structures.
Catalysts 2017, 7, 127 3 of 11 would lead to the collapse of nanotubes, which reveals the larger amount of MPTMS in the precursors could not form nanotubes (Figure S2a).This may be due to the unmatchable hydrolysis and condensation rate between MPTMS with a large amount and phenylene-bridged organosilane, which therefore destroying the self-assembly process.As a comparison, sulfonic pure silica nanotubes without ethenyl or phenylene in the frameworks were also synthesized, which exhibit tube structures in spite of some agglomeration shown in Figure S2b.This also indicated that organic groups in the frameworks could help nanotubes disperse from each other to obtain uniform nanotube structures.The nitrogen adsorption-desorption isotherms of all samples before and after SH oxidation were shown in Figures 2, S3 and S4.Typical type IV isotherms with two hysteresis loops, which represent the interior pore and stacking pores among nanotubes except for SO3H0.5-Ph-SNT,could be observed [15,16].It was found that the Brunauer-Emmett-Teller (BET) surface areas decreased gradually with the sulfur content increasing possibly because of the introduction of mercaptopropyl [17].Using SHx-Ph-SNT as examples, when x changed from 0.1, 0.2 to 0.3, the BET surface areas decreased from 834 m 2 •g −1 , 560 m 2 •g −1 to 530 m 2 •g −1 , respectively.Likewise, pore volume and pore size also declined from 2.1 cm 2 •g −1 and 6.5 nm to 1.2 cm 2 •g −1 and 5.4 nm, respectively (Table S1).After the SH oxidation (seen in Table 1), a similar trend was found and surface areas declined from 537 m 2 •g −1 , 485 m 2 •g −1 to 421 m 2 •g −1 corresponding to x = 0.1, 0.2 and 0.3, while pore sizes stayed the same.Nevertheless, when MPTMS was taken up to 50% in the silica precursors, the hysteresis loop in middle relative pressure can hardly be found (Figure S4), indicating that nanotubes were not formed, which was also confirmed by TEM (Figure S2a).In addition, the BET surface areas were 365 and 231 m 2 •g −1 for SH0.5-Ph-SNT and SO3H0.5-Ph-SNT,respectively.The nitrogen adsorption-desorption isotherms of all samples before and after SH oxidation were shown in Figures 2, S3 and S4.Typical type IV isotherms with two hysteresis loops, which represent the interior pore and stacking pores among nanotubes except for SO 3 H 0.5 -Ph-SNT, could be observed [15,16].It was found that the Brunauer-Emmett-Teller (BET) surface areas decreased gradually with the sulfur content increasing possibly because of the introduction of mercaptopropyl [17].Using SH x -Ph-SNT as examples, when x changed from 0.1, 0.2 to 0.3, the BET surface areas decreased from 834 m 2 •g −1 , 560 m 2 •g −1 to 530 m 2 •g −1 , respectively.Likewise, pore volume and pore size also declined from 2.1 cm 2 •g −1 and 6.5 nm to 1.2 cm 2 •g −1 and 5.4 nm, respectively (Table S1).After the SH oxidation (seen in Table 1), a similar trend was found and surface areas declined from 537 m 2 •g −1 , 485 m 2 •g −1 to 421 m 2 •g −1 corresponding to x = 0.1, 0.2 and 0.3, while pore sizes stayed the same.Nevertheless, when MPTMS was taken up to 50% in the silica precursors, the hysteresis loop in middle relative pressure can hardly be found (Figure S4), indicating that nanotubes were not formed, which was also confirmed by TEM (Figure S2a).In addition, the BET surface areas were 365 and 231 m 2 •g −1 for SH 0.5 -Ph-SNT and SO 3 H 0.5 -Ph-SNT, respectively.
Catalysts 2017, 7, 127 3 of 11 would lead to the collapse of nanotubes, which reveals the larger amount of MPTMS in the precursors could not form nanotubes (Figure S2a).This may be due to the unmatchable hydrolysis and condensation rate between MPTMS with a large amount and phenylene-bridged organosilane, which therefore destroying the self-assembly process.As a comparison, sulfonic pure silica nanotubes without ethenyl or phenylene in the frameworks were also synthesized, which exhibit tube structures in spite of some agglomeration shown in Figure S2b.This also indicated that organic groups in the frameworks could help nanotubes disperse from each other to obtain uniform nanotube structures.The nitrogen adsorption-desorption isotherms of all samples before and after SH oxidation were shown in Figures 2, S3 and S4.Typical type IV isotherms with two hysteresis loops, which represent the interior pore and stacking pores among nanotubes except for SO3H0.5-Ph-SNT,could be observed [15,16].It was found that the Brunauer-Emmett-Teller (BET) surface areas decreased gradually with the sulfur content increasing possibly because of the introduction of mercaptopropyl [17].Using SHx-Ph-SNT as examples, when x changed from 0.1, 0.2 to 0.3, the BET surface areas decreased from 834 m 2 •g −1 , 560 m 2 •g −1 to 530 m 2 •g −1 , respectively.Likewise, pore volume and pore size also declined from 2.1 cm 2 •g −1 and 6.5 nm to 1.2 cm 2 •g −1 and 5.4 nm, respectively (Table S1).After the SH oxidation (seen in Table 1), a similar trend was found and surface areas declined from 537 m 2 •g −1 , 485 m 2 •g −1 to 421 m 2 •g −1 corresponding to x = 0.1, 0.2 and 0.3, while pore sizes stayed the same.Nevertheless, when MPTMS was taken up to 50% in the silica precursors, the hysteresis loop in middle relative pressure can hardly be found (Figure S4), indicating that nanotubes were not formed, which was also confirmed by TEM (Figure S2a).In addition, the BET surface areas were 365 and 231 m 2 •g −1 for SH0.5-Ph-SNT and SO3H0.5-Ph-SNT,respectively.The contents of acidic active sites are measured through the acid-base titration method.After ion exchange with 2 mol/L KCl solution, H + was transferred from solid to the mixture and titrated by 0.01 mol/L sodium hydroxide [18,19].On the basis of titration, acid contents on surface of SO 3 H 0.1 -Et-SNT, SO 3 H 0.2 -Et-SNT, SO 3 H 0.3 -Et-SNT, SO 3 H 0.1 -Ph-SNT, SO 3 H 0.2 -Ph-SNT and SO 3 H 0.3 -Ph-SNT were 0.48, 0.94, 1.96, 0.42, 0.89 and 1.41 mmol•g −1 , respectively.The acid densities on the surface could be calculated to 0.74, 1.67 4.37, 0.78, 1.84, 3.35 µmol•m −2 according to the surface areas of each sample.In order to know if all the -SH groups were oxidized, the X-ray photoelectron spectroscopy (XPS) was used to investigate the oxidation degrees in the solid catalysts.Compared to mercaptopropyl organosilica in which peak at 164 eV assigned to SH groups (Figure S5), a new peak with higher intensity at 169 eV could be detected (Figure 3), corresponding to SO 3 H groups after oxidation [20].However, partial SH also existed after H 2 O 2 treatment due to the unexposed SH groups buried in the tube walls [21].According to the relative ratio of peak areas for SH and SO 3 H, the oxidation degree could be computed and the results ranged from 63% to 81% (Table 1).
SNT, SO3H0.1-Ph-SNT,SO3H0.2-Ph-SNT,SO3H0.3-Ph-SNT.The contents of acidic active sites are measured through the acid-base titration method.After ion exchange with 2 mol/L KCl solution, H + was transferred from solid to the mixture and titrated by 0.01 mol/L sodium hydroxide [18,19].On the basis of titration, acid contents on surface of SO3H0.1-Et-SNT,SO3H0.2-Et-SNT,SO3H0.3-Et-SNT,SO3H0.1-Ph-SNT,SO3H0.2-Ph-SNT and SO3H0.3-Ph-SNT were 0.48, 0.94, 1.96, 0.42, 0.89 and 1.41 mmol•g −1 , respectively.The acid densities on the surface could be calculated to 0.74, 1.67 4.37, 0.78, 1.84, 3.35 μmol•m −2 according to the surface areas of each sample.In order to know if all the -SH groups were oxidized, the X-ray photoelectron spectroscopy (XPS) was used to investigate the oxidation degrees in the solid catalysts.Compared to mercaptopropyl organosilica in which peak at 164 eV assigned to SH groups (Figure S5), a new peak with higher intensity at 169 eV could be detected (Figure 3), corresponding to SO3H groups after oxidation [20].However, partial SH also existed after H2O2 treatment due to the unexposed SH groups buried in the tube walls [21].According to the relative ratio of peak areas for SH and SO3H, the oxidation degree could be computed and the results ranged from 63% to 81% (Table 1).In order to further verify the framework compositions of the organic-inorganic hybrid solid acids, the solid-state 13 C cross polarization magic-angle spinning (CP MAS) and 29 Si magic-angle spinning (MAS) nuclear magnetic resonances (NMR) were conducted using SO3H0.1-Et-SNTand SO3H0.1-Ph-SNT as the samples (Figure 4).In the 13 C NMR spectrum of SO3H0.1-Et-SNT(Figure 4a), a remarkable peak at 5 ppm is attributed to the overlap of bridging ethenyl units and C2 carbon species in propyl group.The other two extremely weak peaks at 18 ppm and 55 ppm, also existing in SO3H0.1-Ph-SNT,could be recognized as C1 and C3 carbon species, respectively.The highest resonance signal at around 135 ppm in Figure 4c belongs to the phenylene units while another obvious resonance at In order to further verify the framework compositions of the organic-inorganic hybrid solid acids, the solid-state 13 C cross polarization magic-angle spinning (CP MAS) and 29 Si magic-angle spinning (MAS) nuclear magnetic resonances (NMR) were conducted using SO 3 H 0.1 -Et-SNT and SO 3 H 0.1 -Ph-SNT as the samples (Figure 4).In the 13 C NMR spectrum of SO 3 H 0.1 -Et-SNT (Figure 4a), a remarkable peak at 5 ppm is attributed to the overlap of bridging ethenyl units and C2 carbon species in propyl group.The other two extremely weak peaks at 18 ppm and 55 ppm, also existing in SO 3 H 0.1 -Ph-SNT, could be recognized as C1 and C3 carbon species, respectively.The highest resonance signal at around 135 ppm in Figure 4c belongs to the phenylene units while another obvious resonance at about 70 ppm was assigned to the remaining template [19,22,23].Regarding the 29

Hydrolysis of Cellobiose
Organic-inorganic composite catalysts with nanotubes structure, SO3Hx-Et-SNT and SO3Hx-Ph-SNT, were expected as efficient catalysts applying in the hydrolysis of cellobiose due to their strong Brønsted acid sites, high acid densities, large specific areas and hollow structures.In all reactions, the mol ratio of acid active sites to substrates was set to 1:14 under the nitrogen pressure of 2.5 MPa at 150 °C.
Figure 5 illustrates time-dependent kinetic curves of SO3Hx-Et-SNT and SO3Hx-Ph-SNT as catalysts, respectively, under the same reaction conditions.Although the amount of acids used in the reaction was kept the same, the reaction activities varied from the different acid densities in the frameworks.The conversion of cellobiose for SO3H0.1-Et-SNT and SO3H0.1-Ph-SNT was 65% and 70% within 2 h, respectively (Table 2).With the increasing of MPTMS to 20%, the conversion rose to 73% and 79% with respect to SO3H0.2-Et-SNT and SO3H0.2-Ph-SNT.As to SO3H0.3-Et-SNT and SO3H0.3-Ph-SNT,conversions reached to 87% and 92%, which were the highest among all the catalysts, suggesting that higher acid density is beneficial to the hydrolysis reaction.Apart from high conversions, SO3Hx-Et-SNT and SO3Hx-Ph-SNT can give at least 89% glucose selectivity within two hours, which is generally higher than other reported results [26,27].Figure S6 shows the liquid chromatography spectra of the reactant and product at the different reaction time using SO3H0.3-Ph-SNTas catalyst, indicating that almost no by-products were formed besides glucose.

Hydrolysis of Cellobiose
Organic-inorganic composite catalysts with nanotubes structure, SO 3 H x -Et-SNT and SO 3 H x -Ph-SNT, were expected as efficient catalysts applying in the hydrolysis of cellobiose due to their strong Brønsted acid sites, high acid densities, large specific areas and hollow structures.In all reactions, the mol ratio of acid active sites to substrates was set to 1:14 under the nitrogen pressure of 2.5 MPa at 150 • C.
Figure 5 illustrates time-dependent kinetic curves of SO 3 H x -Et-SNT and SO 3 H x -Ph-SNT as catalysts, respectively, under the same reaction conditions.Although the amount of acids used in the reaction was kept the same, the reaction activities varied from the different acid densities in the frameworks.The conversion of cellobiose for SO 3 H 0.1 -Et-SNT and SO 3 H 0.1 -Ph-SNT was 65% and 70% within 2 h, respectively (Table 2).With the increasing of MPTMS to 20%, the conversion rose to 73% and 79% with respect to SO 3 H 0.2 -Et-SNT and SO 3 H 0.2 -Ph-SNT.As to SO 3 H 0.3 -Et-SNT and SO 3 H 0.3 -Ph-SNT, conversions reached to 87% and 92%, which were the highest among all the catalysts, suggesting that higher acid density is beneficial to the hydrolysis reaction.Apart from high conversions, SO 3 H x -Et-SNT and SO 3 H x -Ph-SNT can give at least 89% glucose selectivity within two hours, which is generally higher than other reported results [26,27].Figure S6 shows the liquid chromatography spectra of the reactant and product at the different reaction time using SO 3 H 0.3 -Ph-SNT as catalyst, indicating that almost no by-products were formed besides glucose.
Nevertheless, when SO 3 H 0.5 -Ph-SNT synthesized at the proportion of MTPMS in silane precursors of 50% was used, the conversion of cellobiose could only reach 78% within 2 h (Figure 6).The lower activity of SO 3 H 0.5 -Ph-SNT was due to the non-nanotube structure, which was not beneficial to the efficient transport of the reactant or product.In addition, SO 3 H x -Ph-SNT nanotubes always possess a higher conversion compared to SO 3 H x -Et-SNT even with the same acid density.Since phenylene is more hydrophobic than ethenyl as we know, we suppose that the more hydrophobic organic groups in the frameworks may be more suitable for the conversion of cellobiose to glucose.In order to verify the hypothesis, we further synthesized SO 3 H-SNT without any organic groups in the frameworks.
Lower conversion (52%) and yield (46%) were obtained when using the same amount of acid under the same reaction conditions, demonstrating that organic components indeed have positive effects on this reaction (Figure 6).
reaction was kept the same, the reaction activities varied from the different acid densities in the frameworks.The conversion of cellobiose for SO3H0.1-Et-SNT and SO3H0.1-Ph-SNT was 65% and 70% within 2 h, respectively (Table 2).With the increasing of MPTMS to 20%, the conversion rose to 73% and 79% with respect to SO3H0.2-Et-SNT and SO3H0.2-Ph-SNT.As to SO3H0.3-Et-SNT and SO3H0.3-Ph-SNT,conversions reached to 87% and 92%, which were the highest among all the catalysts, suggesting that higher acid density is beneficial to the hydrolysis reaction.Apart from high conversions, SO3Hx-Et-SNT and SO3Hx-Ph-SNT can give at least 89% glucose selectivity within two hours, which is generally higher than other reported results [26,27].Figure S6 shows the liquid chromatography spectra of the reactant and product at the different reaction time using SO3H0.3-Ph-SNTas catalyst, indicating that almost no by-products were formed besides glucose.a Reaction conditions: 50 mL autoclave, 20 mL deionized water, 0.2 g cellobiose, substrates: acid active sites = 14, 150 °C, 2.5 MPa Nitrogen, 800 rpm; b The conversion was obtained within 2 h; c TON = the conversion × the selectivity/the amount of catalyst; d TOF was calculated from the initial reaction rate by using the conversion of cellobiose at 0.5 h.
Nevertheless, when SO3H0.5-Ph-SNT synthesized at the proportion of MTPMS in silane precursors of 50% was used, the conversion of cellobiose could only reach 78% within 2 h (Figure 6).The lower activity of SO3H0.5-Ph-SNT was due to the non-nanotube structure, which was not beneficial to the efficient transport of the reactant or product.In addition, SO3Hx-Ph-SNT nanotubes always possess a higher conversion compared to SO3Hx-Et-SNT even with the same acid density.Since phenylene is more hydrophobic than ethenyl as we know, we suppose that the more hydrophobic organic groups in the frameworks may be more suitable for the conversion of cellobiose to glucose.In order to verify the hypothesis, we further synthesized SO3H-SNT without any organic groups in the frameworks.Lower conversion (52%) and yield (46%) were obtained when using the same amount of acid under the same reaction conditions, demonstrating that organic components indeed have positive effects on this reaction (Figure 6).More interestingly, a relationship between turnover frequency (TOF) and acid density could be found in Figure 7.Under the same reaction conditions, TOF increased to 12.1 h −1 from 8.1 h −1 when the acid density was changed to 3.35 from 0.78 μmol•m −2 for SO3Hx-Ph-SNT, which suggests that the synergetic effect among acid sites might existed.Yang's group reported the synergetic effect among SO3H groups and they speculated that formation of H-bond interactions derived from closer acid sites would enhance acid strength, therefore increasing the cooperation effect [28].
The reusability of the acid catalysts was investigated using SO3H0.3-Ph-SNTas catalysts and the results are showed in Figure 8.Although the conversion of cellobiose and the yield of glucose More interestingly, a relationship between turnover frequency (TOF) and acid density could be found in Figure 7.Under the same reaction conditions, TOF increased to 12.1 h −1 from 8.1 h −1 when the acid density was changed to 3.35 from 0.78 µmol•m −2 for SO 3 H x -Ph-SNT, which suggests that the synergetic effect among acid sites might existed.Yang's group reported the synergetic effect among SO 3 H groups and they speculated that formation of H-bond interactions derived from closer acid sites would enhance acid strength, therefore increasing the cooperation effect [28].
Catalysts 2017, 7, 127 7 of 11 decreased to 83% and 75% for the second run, it still exhibited good performance for the sixth reuse with the conversion of 66% and the yield of 59%.After the reaction, we investigated the sulfur leaching in the solution.According to the data from ICP-MS, around 30% sulfur leached during the reaction, which might lead to the decreased activity during the recycling.However, the leaching amount was somewhat lower than those of other catalysts systems reported [29].The reason for this might be the existence of organic groups in the frameworks, which could bear the harsh conditions during the hydrolysis reaction.In order to investigate reaction temperature effect on this system, reactions using SO3H0.1-Ph-SNTas catalysts at different temperatures were carried out and the results were shown in Figure S7.At 130 °C, both conversion and yield were lower than 40%, even though the selectivity was close to 100%.The gap between the conversion and the yield was widened gradually with the increasing of the temperature, indicating that higher reaction temperature has an adverse impact on glucose selectivity.At 160 °C, the conversion reached nearly 100% and the selectivity of glucose was 88% within 2 h.

Chemicals and Regents
1,2-bis(trimethoxysilyl)ethane (BTME), 1,4-bis(triethoxysilyl)benzene (BTEB), 3mercaptopropyltrimethoxysilane (MPTMS), tetraethyl orthosilicate (TEOS) and potassium chloride were purchased from J&K Scientific Ltd., Beijing, China.Hydrochloric acid (37%), sulfuric acid (98%) and ethanol were obtained from Real&Lead Chemical Co. Ltd., Tianjin, China.Hydrogen peroxide (40%), cellobiose and Pluronic P123 were received from Sigma-Aldrich, St. Louis, MO, USA.All reagents were used without further purification.Chromatogram analysis was carried out on N2000 chromatographic work station, Version 3.20, Zhejiang University, Hangzhou, China.The reusability of the acid catalysts was investigated using SO 3 H 0.3 -Ph-SNT as catalysts and the results are showed in Figure 8.Although the conversion of cellobiose and the yield of glucose decreased to 83% and 75% for the second run, it still exhibited good performance for the sixth reuse with the conversion of 66% and the yield of 59%.After the reaction, we investigated the sulfur leaching in the solution.According to the data from ICP-MS, around 30% sulfur leached during the reaction, which might lead to the decreased activity during the recycling.However, the leaching amount was somewhat lower than those of other catalysts systems reported [29].The reason for this might be the existence of organic groups in the frameworks, which could bear the harsh conditions during the hydrolysis reaction.decreased to 83% and 75% for the second run, it still exhibited good performance for the sixth reuse with the conversion of 66% and the yield of 59%.After the reaction, we investigated the sulfur leaching in the solution.According to the data from ICP-MS, around 30% sulfur leached during the reaction, which might lead to the decreased activity during the recycling.However, the leaching amount was somewhat lower than those of other catalysts systems reported [29].The reason for this might be the existence of organic groups in the frameworks, which could bear the harsh conditions during the hydrolysis reaction.In order to investigate reaction temperature effect on this system, reactions using SO3H0.1-Ph-SNTas catalysts at different temperatures were carried out and the results were shown in Figure S7.At 130 °C, both conversion and yield were lower than 40%, even though the selectivity was close to 100%.The gap between the conversion and the yield was widened gradually with the increasing of the temperature, indicating that higher reaction temperature has an adverse impact on glucose selectivity.At 160 °C, the conversion reached nearly 100% and the selectivity of glucose was 88% within 2 h.

Chemicals and Regents
1,2-bis(trimethoxysilyl)ethane (BTME), 1,4-bis(triethoxysilyl)benzene (BTEB), 3mercaptopropyltrimethoxysilane (MPTMS), tetraethyl orthosilicate (TEOS) and potassium chloride were purchased from J&K Scientific Ltd., Beijing, China.Hydrochloric acid (37%), sulfuric acid (98%) and ethanol were obtained from Real&Lead Chemical Co. Ltd., Tianjin, China.Hydrogen peroxide (40%), cellobiose and Pluronic P123 were received from Sigma-Aldrich, St. Louis, MO, USA.All reagents were used without further purification.Chromatogram analysis was carried out on N2000 chromatographic work station, Version 3.20, Zhejiang University, Hangzhou, China.In order to investigate reaction temperature effect on this system, reactions using SO 3 H 0.1 -Ph-SNT as catalysts at different temperatures were carried out and the results were shown in Figure S7.At 130 • C, both conversion and yield were lower than 40%, even though the selectivity was close to 100%.The gap between the conversion and the yield was widened gradually with the increasing of the temperature, indicating that higher reaction temperature has an adverse impact on glucose selectivity.At 160 • C, the conversion reached nearly 100% and the selectivity of glucose was 88% within 2 h.

Synthesis of Sulfonic Ethyl-Bridged Organosilica Nanotubes
According to our previous work [10] and a method reported by Wang [18], sulfonic organosilica nanotubes bridged with ethenyl in the framework were synthesized as follows: Typically, a certain amount of BTME was added dropwise into 135 mL of hydrochloric acid (2 mol/L) containing 1.75 g of potassium chloride and 0.55 g of P123 with vigorous stirring at 38 • C. The mixture was kept static for 12 h before the addition of MPTMS.The total precursor amounts based on Si were 7 mmol and MPTMS was adjusted to 10%, 20%, 30% and 50%, respectively.After being stirred for 24 h, the mixture was subsequently transferred to a BTFE bottle and aged at 100 • C for 24 h under static conditions.The product was collected by filtration, washed by deionized water and dried at room temperature.Surfactants were removed by extraction in ethanol and HCl under refluxing.The obtained samples were named as SH x -Et-SNT (x means the ratio of MPTMS amounts in the total precursors, Et stands for ethenyl groups in the frameworks and SNT is the abbreviation of silica nanotubes).
In order to oxidize SH to SO 3 H, 0.5 g of SH x -Et-SNT was mixed with 20 mL of H 2 O 2 (30%) under stirring for 12 h at room temperature, following the treatment of 200 mL H 2 SO 4 (0.1 M) for another 12 h.Finally, the suspension was filtered and continually washed by deionized water until the filtrate was neutral.The solids were denoted as SO 3 H x -Et-SNT.

Synthesis of Sulfonic Phenyl-Bridged Organosilica Nanotubes
The synthesis process of SH-Ph-SNT and SO 3 H x -Ph-SNT (Ph stands for phenylene groups in the frameworks) was similar with that of SH-Et-SNT and SO 3 H x -Et-SNT with minor changes.After complete dissolution of 0.275 g of P123 and 0.875 g of KCl in 90 mL of hydrochloric acid (2 mol/L), BTEB was added into the solution drop by drop.Then the solution was continually stirred for 12 h followed by addition of MPTMS.The precursor amounts and the procedures including hydrothermal treatment, extraction of surfactants and SH oxidation were the same as those of SO 3 H x -Et-SNT.

Synthesis of Sulfonic Silica Nanotubes
The synthesis of sulfonic silica nanotubes followed the procedure reported by Yang with a minor revision [30].Typically, 0.4 g of P123 and 12 g of boric acid were completely dissolved in 160 mL of deionized water with stirring at 40 • C.After that, 1.8 mL of TEOS was dropped into the solution and kept stirring for 12 h followed by addition of 0.2 mL of MPTMS.Then, the mixture was continually stirred at 40 • C for 80 h with gentle stirring and subsequently transferred to a PTFE bottle to be aged at 100 • C for 24 h.Then the mixture was undertaken the same progress with SO 3 H x -Et-SNT, including the template extraction and SH oxidation.The obtained material with pure silica frameworks was named as SH-SNT and SO 3 H-SNT, respectively, before and after SH oxidation.

Characterization
The transmission electron microscopy (TEM) images were taken on a FEI Tecnai G2 F20 electron microscope operated at an acceleration voltage of 200 kV.The nitrogen adsorption-desorption isotherms were measured on Tristar 3000 (Micrometrics) after the samples were outgassed at 120 • C for 3 h.The Brunauer-Emmett-Teller (BET) specific surface areas were calculated using adsorption data at the relative pressure range of P/P 0 = 0.05-0.25.Pore size distributions were calculated from adsorption branch using the Barrett-Joyner-Halenda (BJH) method.Sulfur content in hybrid catalysts was performed on an Elementar Vario Micro cube elemental analyzer (CHNS mode), Lyon, France. 13

Catalytic Test
Hydrolysis of cellobiose was carried out in a steal autoclave equipped with a magnetic stirrer.Typically, 0.20 g of cellobiose, a certain amount of catalysts (substrates: active sites = 14) and 20 mL deionized water were added into an autoclave in order.Then, the sealed system was flushed with nitrogen at least 3 times to remove air and finally pressurized to 2.5 MPa.The reactions were carried out at 150 • C for 30 min, 90 min and 120 min, respectively, with magnetic stirring at 800 r/min.To stop the reaction, an icy cold atmosphere was required and after that, the solid catalysts were separated by centrifugation.Liquid phase mixture was analyzed by high performance liquid chromatography (HPLC) equipped with an ICSep ICE-Coregel 87H3 column and a RID detector.The HPLC column was retained at 38 • C, using H 2 SO 4 solution (5 mmol/L, 0.6 mL/min) as the mobile phase.Conversions and yields were calculated through calibration curves obtained from external standards method and the equations are showed as blow:

Conclusions
Ethenyl-or phenylene-bridged sulfonic organosilica nanotubes with different acid contents were synthesized through the -SH oxidation of sulfhydryl organosilica nanotubes, which were prepared through an easy soft-template method.The samples exhibited obvious hollow tube structures with pore diameters of about 5 nm evidenced by TEM images and nitrogen adsorption-desorption isotherms.XPS showed about 63% to 81% of -SH groups in the solids were oxidized to -SO 3 H and acid contents were ascertained by acid-base titration method.The novel solid acid catalysts exhibited excellent activities in the hydrolysis of cellobiose with the highest conversion of 92% and glucose selectivity of 96% for SO 3 H 0.3 -Ph-SNT.Both surface acid densities and organic groups in the nanotube frameworks play important roles on this reaction.Furthermore, the catalysts could be reused at least six times and still showed high activities with the conversion of 66% and the glucose selectivity of 92%.This study provides a new series of sulfonic organosilica nanotubes with different acid densities for the hydrolysis of cellobiose, which have not been reported before.S6: Liquid chromatography spectra of mixture including cellubiose (green square) and glucose (orange circle) after reaction using SO 3 H 0.3 -Ph-SNT as catalyst, Figure S7: Hydrolysis of cellubiose by using SO 3 H 0.1 -Ph-SNT as catalysts at 130 • C, 140 • C, 150 • C and 160 • C, respectively.Reaction conditions: 50 mL autoclave, 20 mL deionized water, substrates: acid active sites = 14, 0.2 g cellubiose, 2.5 MPa Nitrogen, 800 rpm, 2 h.Table S1: Physicochemical properties of SH x -Et-SNT and SH x -Ph-SNT.

Figure 7 .
Figure 7. Relationship between turnover frequency (TOF) and acid density for SO 3 H x -Ph-SNT and SO 3 H x -Et-SNT.

Table 1 .
Physicochemical properties of various catalysts with different sulfur contents and bridged groups.Surface area was determined using the Brunauer-Emmett-Teller (BET) model; b Pore size was estimated Barrett-Joyner-Halenda (BJH) method with adsorption branch; c Acid content was verified by acid-base titration; d Acid density was calculated from acid contents (acid-base titration) divided by BET surface areas; e Oxidation degree was determined by XPS characterization. a

Table 1 .
Physicochemical properties of various catalysts with different sulfur contents and bridged groups.Surface area was determined using the Brunauer-Emmett-Teller (BET) model; b Pore size was estimated Barrett-Joyner-Halenda (BJH) method with adsorption branch; c Acid content was verified by acid-base titration; d Acid density was calculated from acid contents (acid-base titration) divided by BET surface areas; e Oxidation degree was determined by XPS characterization. a

Table 2 .
Catalytic performance of several sulfonic organosilica nanotubes towards hydrolysis of cellobiose a .

Table 2 .
Catalytic performance of several sulfonic organosilica nanotubes towards hydrolysis of cellobiose a .